Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000967.01 | MsG0780039829.01 | 0.958307 | 3.255030e-116 | 1.194848e-110 |
| MsG0180000967.01 | MsG0880045028.01 | 0.853486 | 2.315029e-61 | 5.010045e-58 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTCAGGTCCCATCCTTTC+AGG | 0.164802 | 1:-13861572 | MsG0180000967.01.T01:CDS |
| TTTATTTCATTCACTTGTTT+TGG | 0.234633 | 1:-13861600 | MsG0180000967.01.T01:CDS |
| ATATAGATGATGGGTTTGAT+TGG | 0.393563 | 1:-13861476 | MsG0180000967.01.T01:CDS |
| GATGATGGGTTTGATTGGTT+TGG | 0.407984 | 1:-13861471 | MsG0180000967.01.T01:CDS |
| GGTTTGGTGATGAAGTTTGC+TGG | 0.429867 | 1:-13861455 | MsG0180000967.01.T01:CDS |
| TTGACAAATTCCTGAAAGGA+TGG | 0.507451 | 1:+13861562 | None:intergenic |
| TTTGGTTACATATAGATGAT+GGG | 0.522179 | 1:-13861485 | MsG0180000967.01.T01:CDS |
| AGACGCATTGAGAAAATCAA+AGG | 0.589980 | 1:-13861669 | MsG0180000967.01.T01:CDS |
| AGGACGACGTTGATTGTGAA+AGG | 0.596660 | 1:-13861390 | MsG0180000967.01.T01:CDS |
| GAATTCAAACACAAAATCAT+CGG | 0.605670 | 1:+13861530 | None:intergenic |
| ACAGTTGACAAATTCCTGAA+AGG | 0.607677 | 1:+13861558 | None:intergenic |
| TGACAAATTCCTGAAAGGAT+GGG | 0.614992 | 1:+13861563 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTATTTCATTCACTTGTTT+TGG | - | Chr1:13861506-13861525 | MsG0180000967.01.T01:CDS | 20.0% |
| ! | GAATTCAAACACAAAATCAT+CGG | + | Chr1:13861579-13861598 | None:intergenic | 25.0% |
| ! | TTTGGTTACATATAGATGAT+GGG | - | Chr1:13861621-13861640 | MsG0180000967.01.T01:CDS | 25.0% |
| !!! | CTTGCTATGATTTTCAATTT+TGG | - | Chr1:13861603-13861622 | MsG0180000967.01.T01:CDS | 25.0% |
| !!! | TCTTGATTTTTGGAAGAAAA+AGG | - | Chr1:13861696-13861715 | MsG0180000967.01.T01:CDS | 25.0% |
| !!! | TTTTGGTTACATATAGATGA+TGG | - | Chr1:13861620-13861639 | MsG0180000967.01.T01:CDS | 25.0% |
| TCCAAAAATCAAGAATGCAA+AGG | + | Chr1:13861690-13861709 | None:intergenic | 30.0% | |
| ! | TCCTTTGCATTCTTGATTTT+TGG | - | Chr1:13861686-13861705 | MsG0180000967.01.T01:CDS | 30.0% |
| !! | ATATAGATGATGGGTTTGAT+TGG | - | Chr1:13861630-13861649 | MsG0180000967.01.T01:CDS | 30.0% |
| ACAGTTGACAAATTCCTGAA+AGG | + | Chr1:13861551-13861570 | None:intergenic | 35.0% | |
| AGACGCATTGAGAAAATCAA+AGG | - | Chr1:13861437-13861456 | MsG0180000967.01.T01:CDS | 35.0% | |
| TGACAAATTCCTGAAAGGAT+GGG | + | Chr1:13861546-13861565 | None:intergenic | 35.0% | |
| TTGACAAATTCCTGAAAGGA+TGG | + | Chr1:13861547-13861566 | None:intergenic | 35.0% | |
| !!! | CTTGTTTTGGTTCAATTCTC+AGG | - | Chr1:13861519-13861538 | MsG0180000967.01.T01:CDS | 35.0% |
| !! | GATGATGGGTTTGATTGGTT+TGG | - | Chr1:13861635-13861654 | MsG0180000967.01.T01:CDS | 40.0% |
| ! | AGGACGACGTTGATTGTGAA+AGG | - | Chr1:13861716-13861735 | MsG0180000967.01.T01:CDS | 45.0% |
| !! | GGTTTGGTGATGAAGTTTGC+TGG | - | Chr1:13861651-13861670 | MsG0180000967.01.T01:CDS | 45.0% |
| TTCTCAGGTCCCATCCTTTC+AGG | - | Chr1:13861534-13861553 | MsG0180000967.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 13861380 | 13861748 | 13861380 | ID=MsG0180000967.01;Name=MsG0180000967.01 |
| Chr1 | mRNA | 13861380 | 13861748 | 13861380 | ID=MsG0180000967.01.T01;Parent=MsG0180000967.01;Name=MsG0180000967.01.T01;_AED=0.89;_eAED=0.89;_QI=0|-1|0|1|-1|0|1|0|122 |
| Chr1 | exon | 13861380 | 13861748 | 13861380 | ID=MsG0180000967.01.T01:exon:7690;Parent=MsG0180000967.01.T01 |
| Chr1 | CDS | 13861380 | 13861748 | 13861380 | ID=MsG0180000967.01.T01:cds;Parent=MsG0180000967.01.T01 |
| Gene Sequence |
| Protein sequence |
