Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000967.01 | MsG0880045028.01 | 0.853486 | 2.315029e-61 | 5.010045e-58 |
| MsG0680030906.01 | MsG0880045028.01 | 0.807399 | 5.151511e-50 | 2.866484e-47 |
| MsG0780039829.01 | MsG0880045028.01 | 0.842011 | 3.354858e-58 | 5.018493e-55 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTCAGGTCCCATCCTTTC+AGG | 0.164802 | 8:-52917199 | MsG0880045028.01.T01:CDS |
| TTTATTTCATTCACTTGTTT+TGG | 0.234633 | 8:-52917227 | MsG0880045028.01.T01:CDS |
| ATATAGATGATGGGTTTGAT+TGG | 0.393563 | 8:-52917103 | MsG0880045028.01.T01:CDS |
| GATGATGGGTTTGATTGGTT+TGG | 0.407984 | 8:-52917098 | MsG0880045028.01.T01:CDS |
| GGTTTGGTGATGAAGTTTGC+TGG | 0.429867 | 8:-52917082 | MsG0880045028.01.T01:CDS |
| TTGACAAATTCCTGAAAGGA+TGG | 0.507451 | 8:+52917189 | None:intergenic |
| TTTGGTTACATATAGATGAT+GGG | 0.522179 | 8:-52917112 | MsG0880045028.01.T01:CDS |
| AGGACGAAGTTGATTGTGAA+AGG | 0.570910 | 8:-52917017 | MsG0880045028.01.T01:CDS |
| AGACGCATTGAGAAAATCAA+AGG | 0.589980 | 8:-52917296 | MsG0880045028.01.T01:CDS |
| GAATTCAAACACAAAATCAT+CGG | 0.605670 | 8:+52917157 | None:intergenic |
| ACAGTTGACAAATTCCTGAA+AGG | 0.607677 | 8:+52917185 | None:intergenic |
| TGACAAATTCCTGAAAGGAT+GGG | 0.614992 | 8:+52917190 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTATTTCATTCACTTGTTT+TGG | - | Chr8:52917133-52917152 | MsG0880045028.01.T01:CDS | 20.0% |
| ! | GAATTCAAACACAAAATCAT+CGG | + | Chr8:52917206-52917225 | None:intergenic | 25.0% |
| ! | TTTGGTTACATATAGATGAT+GGG | - | Chr8:52917248-52917267 | MsG0880045028.01.T01:CDS | 25.0% |
| !!! | CTTGCTATGATTTTCAATTT+TGG | - | Chr8:52917230-52917249 | MsG0880045028.01.T01:CDS | 25.0% |
| !!! | TCTTGATTTTTGGAAGAAAA+AGG | - | Chr8:52917323-52917342 | MsG0880045028.01.T01:CDS | 25.0% |
| !!! | TTTTGGTTACATATAGATGA+TGG | - | Chr8:52917247-52917266 | MsG0880045028.01.T01:CDS | 25.0% |
| TCCAAAAATCAAGAATGCAA+AGG | + | Chr8:52917317-52917336 | None:intergenic | 30.0% | |
| ! | TCCTTTGCATTCTTGATTTT+TGG | - | Chr8:52917313-52917332 | MsG0880045028.01.T01:CDS | 30.0% |
| !! | ATATAGATGATGGGTTTGAT+TGG | - | Chr8:52917257-52917276 | MsG0880045028.01.T01:CDS | 30.0% |
| ACAGTTGACAAATTCCTGAA+AGG | + | Chr8:52917178-52917197 | None:intergenic | 35.0% | |
| AGACGCATTGAGAAAATCAA+AGG | - | Chr8:52917064-52917083 | MsG0880045028.01.T01:CDS | 35.0% | |
| TGACAAATTCCTGAAAGGAT+GGG | + | Chr8:52917173-52917192 | None:intergenic | 35.0% | |
| TTGACAAATTCCTGAAAGGA+TGG | + | Chr8:52917174-52917193 | None:intergenic | 35.0% | |
| !!! | CTTGTTTTGGTTCAATTCTC+AGG | - | Chr8:52917146-52917165 | MsG0880045028.01.T01:CDS | 35.0% |
| ! | AGGACGAAGTTGATTGTGAA+AGG | - | Chr8:52917343-52917362 | MsG0880045028.01.T01:CDS | 40.0% |
| !! | GATGATGGGTTTGATTGGTT+TGG | - | Chr8:52917262-52917281 | MsG0880045028.01.T01:CDS | 40.0% |
| !! | GGTTTGGTGATGAAGTTTGC+TGG | - | Chr8:52917278-52917297 | MsG0880045028.01.T01:CDS | 45.0% |
| TTCTCAGGTCCCATCCTTTC+AGG | - | Chr8:52917161-52917180 | MsG0880045028.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 52917007 | 52917375 | 52917007 | ID=MsG0880045028.01;Name=MsG0880045028.01 |
| Chr8 | mRNA | 52917007 | 52917375 | 52917007 | ID=MsG0880045028.01.T01;Parent=MsG0880045028.01;Name=MsG0880045028.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|122 |
| Chr8 | exon | 52917007 | 52917375 | 52917007 | ID=MsG0880045028.01.T01:exon:7042;Parent=MsG0880045028.01.T01 |
| Chr8 | CDS | 52917007 | 52917375 | 52917007 | ID=MsG0880045028.01.T01:cds;Parent=MsG0880045028.01.T01 |
| Gene Sequence |
| Protein sequence |
