Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000967.01 | MsG0780039829.01 | 0.958307 | 3.255030e-116 | 1.194848e-110 |
| MsG0180001410.01 | MsG0780039829.01 | 0.811171 | 8.001840e-51 | 4.918242e-48 |
| MsG0180002089.01 | MsG0780039829.01 | 0.803754 | 2.997597e-49 | 1.518666e-46 |
| MsG0180002282.01 | MsG0780039829.01 | 0.802042 | 6.769260e-49 | 3.282562e-46 |
| MsG0180004264.01 | MsG0780039829.01 | 0.806967 | 6.359953e-50 | 3.499310e-47 |
| MsG0380012702.01 | MsG0780039829.01 | 0.805621 | 1.221963e-49 | 6.495280e-47 |
| MsG0680032801.01 | MsG0780039829.01 | 0.805255 | 1.458206e-49 | 7.678115e-47 |
| MsG0480022259.01 | MsG0780039829.01 | 0.808470 | 3.048780e-50 | 1.744750e-47 |
| MsG0480022262.01 | MsG0780039829.01 | 0.800240 | 1.581331e-48 | 7.326134e-46 |
| MsG0380016475.01 | MsG0780039829.01 | 0.809438 | 1.892228e-50 | 1.110987e-47 |
| MsG0780039829.01 | MsG0880045028.01 | 0.842011 | 3.354858e-58 | 5.018493e-55 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTCAGGTCCCATCCTTTC+AGG | 0.164802 | 7:-69534009 | MsG0780039829.01.T01:CDS |
| TTTATTTCATTCACTTGTTT+TGG | 0.234633 | 7:-69534037 | MsG0780039829.01.T01:CDS |
| ATATAGATGATGGGTTTGAT+TGG | 0.393563 | 7:-69533913 | MsG0780039829.01.T01:CDS |
| GATGATGGGTTTGATTGGTT+TGG | 0.407984 | 7:-69533908 | MsG0780039829.01.T01:CDS |
| GGTTTGGTGATGAAGTTTGC+TGG | 0.429867 | 7:-69533892 | MsG0780039829.01.T01:CDS |
| TTGACAAATTCCTGAAAGGA+TGG | 0.507451 | 7:+69533999 | None:intergenic |
| TTTGGTTACATATAGATGAT+GGG | 0.522179 | 7:-69533922 | MsG0780039829.01.T01:CDS |
| AGACGCATTGAGAAAATCAA+AGG | 0.589980 | 7:-69534106 | MsG0780039829.01.T01:CDS |
| AGGACGACGTTGATTGTGAA+AGG | 0.596660 | 7:-69533827 | MsG0780039829.01.T01:CDS |
| GAATTCAAACACAAAATCAT+CGG | 0.605670 | 7:+69533967 | None:intergenic |
| ACAGTTGACAAATTCCTGAA+AGG | 0.607677 | 7:+69533995 | None:intergenic |
| TGACAAATTCCTGAAAGGAT+GGG | 0.614992 | 7:+69534000 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTATTTCATTCACTTGTTT+TGG | - | Chr7:69533943-69533962 | MsG0780039829.01.T01:CDS | 20.0% |
| ! | GAATTCAAACACAAAATCAT+CGG | + | Chr7:69534016-69534035 | None:intergenic | 25.0% |
| ! | TTTGGTTACATATAGATGAT+GGG | - | Chr7:69534058-69534077 | MsG0780039829.01.T01:CDS | 25.0% |
| !!! | CTTGCTATGATTTTCAATTT+TGG | - | Chr7:69534040-69534059 | MsG0780039829.01.T01:CDS | 25.0% |
| !!! | TCTTGATTTTTGGAAGAAAA+AGG | - | Chr7:69534133-69534152 | MsG0780039829.01.T01:CDS | 25.0% |
| !!! | TTTTGGTTACATATAGATGA+TGG | - | Chr7:69534057-69534076 | MsG0780039829.01.T01:CDS | 25.0% |
| TCCAAAAATCAAGAATGCAA+AGG | + | Chr7:69534127-69534146 | None:intergenic | 30.0% | |
| ! | TCCTTTGCATTCTTGATTTT+TGG | - | Chr7:69534123-69534142 | MsG0780039829.01.T01:CDS | 30.0% |
| !! | ATATAGATGATGGGTTTGAT+TGG | - | Chr7:69534067-69534086 | MsG0780039829.01.T01:CDS | 30.0% |
| ACAGTTGACAAATTCCTGAA+AGG | + | Chr7:69533988-69534007 | None:intergenic | 35.0% | |
| AGACGCATTGAGAAAATCAA+AGG | - | Chr7:69533874-69533893 | MsG0780039829.01.T01:CDS | 35.0% | |
| TGACAAATTCCTGAAAGGAT+GGG | + | Chr7:69533983-69534002 | None:intergenic | 35.0% | |
| TTGACAAATTCCTGAAAGGA+TGG | + | Chr7:69533984-69534003 | None:intergenic | 35.0% | |
| !!! | CTTGTTTTGGTTCAATTCTC+AGG | - | Chr7:69533956-69533975 | MsG0780039829.01.T01:CDS | 35.0% |
| !! | GATGATGGGTTTGATTGGTT+TGG | - | Chr7:69534072-69534091 | MsG0780039829.01.T01:CDS | 40.0% |
| ! | AGGACGACGTTGATTGTGAA+AGG | - | Chr7:69534153-69534172 | MsG0780039829.01.T01:CDS | 45.0% |
| !! | GGTTTGGTGATGAAGTTTGC+TGG | - | Chr7:69534088-69534107 | MsG0780039829.01.T01:CDS | 45.0% |
| TTCTCAGGTCCCATCCTTTC+AGG | - | Chr7:69533971-69533990 | MsG0780039829.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 69533817 | 69534185 | 69533817 | ID=MsG0780039829.01;Name=MsG0780039829.01 |
| Chr7 | mRNA | 69533817 | 69534185 | 69533817 | ID=MsG0780039829.01.T01;Parent=MsG0780039829.01;Name=MsG0780039829.01.T01;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|122 |
| Chr7 | exon | 69533817 | 69534185 | 69533817 | ID=MsG0780039829.01.T01:exon:15124;Parent=MsG0780039829.01.T01 |
| Chr7 | CDS | 69533817 | 69534185 | 69533817 | ID=MsG0780039829.01.T01:cds;Parent=MsG0780039829.01.T01 |
| Gene Sequence |
| Protein sequence |
