AlfalfaGEDB | MsG0180000971.01

M. sativa cultivar ZhongmuNo.1 / MsG0180000971.01

KEGG: -
GO: GO:0004672 GO:0005524 GO:0006468
PFAM: PF00069

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000971.01.T01	XP_024631253.2	83.821	581	86	5	1	574	1	580	0	992

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000971.01.T01	P93604	48.219	365	180	5	198	556	266	627	2.13E-117	365

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000971.01.T01	A0A396JKR8	83.821	581	86	5	1	574	1	580	0.0	992

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000931.01	MsG0180000971.01	0.880074	7.404662e-70	4.114944e-66
MsG0180000949.01	MsG0180000971.01	0.850583	1.546212e-60	3.043084e-57
MsG0180000961.01	MsG0180000971.01	0.814774	1.298449e-51	8.791378e-49
MsG0180000971.01	MsG0180001281.01	0.810195	1.300619e-50	7.789633e-48
MsG0180000971.01	MsG0480022065.01	0.800158	1.643539e-48	7.598179e-46
MsG0180000971.01	MsG0680034782.01	0.807180	5.733041e-50	3.171875e-47
MsG0180000971.01	MsG0780039438.01	0.804383	2.217450e-49	1.141729e-46

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MsG0180000971.01.T01	MTR_1g027960	84.536	582	81	6	1	574	1	581	981
MsG0180000971.01.T01	MTR_6g445600	83.275	574	88	3	1	574	1	566	976
MsG0180000971.01.T01	MTR_0690s0010	80.662	574	77	4	1	574	1	540	934
MsG0180000971.01.T01	MTR_1g028220	70.775	568	150	7	22	584	18	574	786
MsG0180000971.01.T01	MTR_1g027820	68.143	587	160	13	8	584	2	571	781
MsG0180000971.01.T01	MTR_1g027150	63.972	569	189	8	20	584	16	572	736
MsG0180000971.01.T01	MTR_1g027890	84.000	425	46	3	152	574	1	405	734
MsG0180000971.01.T01	MTR_3g028650	67.018	570	163	12	24	584	20	573	721
MsG0180000971.01.T01	MTR_1g027040	63.540	565	183	8	28	584	26	575	716
MsG0180000971.01.T01	MTR_1g027060	60.457	569	156	8	20	584	16	519	668

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000971.01.T01	AT1G66910	45.480	354	177	7	212	555	284	631	5.72e-99	315
MsG0180000971.01.T01	AT1G66910	45.480	354	177	7	212	555	287	634	7.38e-99	315
MsG0180000971.01.T01	AT1G70250	41.176	425	227	12	141	556	338	748	1.23e-97	315
MsG0180000971.01.T01	AT1G66920	49.498	299	145	5	261	555	296	592	4.89e-97	308
MsG0180000971.01.T01	AT1G66920	49.498	299	145	5	261	555	288	584	8.92e-97	308
MsG0180000971.01.T01	AT5G38260	45.402	348	175	7	214	555	269	607	5.48e-95	304
MsG0180000971.01.T01	AT1G66980	49.834	301	141	4	262	555	778	1075	2.66e-94	312
MsG0180000971.01.T01	AT1G66980	49.834	301	141	4	262	555	795	1092	3.88e-94	312
MsG0180000971.01.T01	AT1G66980	49.834	301	141	4	262	555	824	1121	6.17e-94	312
MsG0180000971.01.T01	AT1G66980	49.834	301	141	4	262	555	822	1119	7.77e-94	311

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Find 118 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTCAATCTCATTATCA+TGG	0.519234	1:+13889354	MsG0180000971.01.T01:CDS
AAAAGATGATTTCCTTGGAT+GGG	0.505661	1:+13890014	MsG0180000971.01.T01:CDS
AAAGTACCAACTAATCCTTT+CGG	0.527532	1:+13890579	MsG0180000971.01.T01:CDS
AAATCTCCCTCTTCATCAAT+AGG	0.321382	1:-13890441	None:intergenic
AAGATAGGCGAAGGGACTCA+TGG	0.559971	1:+13889760	MsG0180000971.01.T01:CDS
AAGATTAGTGTAGCCTCTGC+AGG	0.491106	1:+13889431	MsG0180000971.01.T01:CDS
AAGGAATTTATAAATGAAGT+GGG	0.542338	1:+13889859	MsG0180000971.01.T01:CDS
AAGTATTGTATCCATGACTG+CGG	0.704791	1:+13890209	MsG0180000971.01.T01:CDS
AAGTCCGATATATACAGTTA+CGG	0.539938	1:+13890297	MsG0180000971.01.T01:CDS
AAGTTGCAACAAATTGCTCT+AGG	0.589174	1:+13890039	MsG0180000971.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAGATGATTTCCTTGGA+TGG	+	Chr1:13890013-13890032	MsG0180000971.01.T01:CDS	30.0%
AAAAGATGATTTCCTTGGAT+GGG	+	Chr1:13890014-13890033	MsG0180000971.01.T01:CDS	30.0%
AAGTCCGATATATACAGTTA+CGG	+	Chr1:13890297-13890316	MsG0180000971.01.T01:CDS	30.0%
AGAAAGAATACAAAAACGAC+CGG	+	Chr1:13890348-13890367	MsG0180000971.01.T01:CDS	30.0%
ATTCACTTCTCTACTTCATT+TGG	+	Chr1:13889462-13889481	MsG0180000971.01.T01:intron	30.0%
ATTGTGCTTCTTCTTCTATT+TGG	+	Chr1:13889592-13889611	MsG0180000971.01.T01:CDS	30.0%
CACATTCATGTGAAAGAATA+AGG	-	Chr1:13889319-13889338	None:intergenic	30.0%
CATACCGTAACTGTATATAT+CGG	-	Chr1:13890304-13890323	None:intergenic	30.0%
CATTCATGTGAAAGAATAAG+GGG	-	Chr1:13889317-13889336	None:intergenic	30.0%
CTTAATAATACAGAGGAAGA+TGG	+	Chr1:13889835-13889854	MsG0180000971.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	13888783	13890840	13888783	ID=MsG0180000971.01;Name=MsG0180000971.01
Chr1	mRNA	13888783	13890840	13888783	ID=MsG0180000971.01.T01;Parent=MsG0180000971.01;Name=MsG0180000971.01.T01;_AED=0.03;_eAED=0.03;_QI=27\|1\|1\|1\|1\|1\|2\|154\|584
Chr1	exon	13888783	13889452	13888783	ID=MsG0180000971.01.T01:exon:9507;Parent=MsG0180000971.01.T01
Chr1	exon	13889575	13890840	13889575	ID=MsG0180000971.01.T01:exon:9508;Parent=MsG0180000971.01.T01
Chr1	five_prime_UTR	13888783	13888809	13888783	ID=MsG0180000971.01.T01:five_prime_utr;Parent=MsG0180000971.01.T01
Chr1	CDS	13888810	13889452	13888810	ID=MsG0180000971.01.T01:cds;Parent=MsG0180000971.01.T01
Chr1	CDS	13889575	13890686	13889575	ID=MsG0180000971.01.T01:cds;Parent=MsG0180000971.01.T01
Chr1	three_prime_UTR	13890687	13890840	13890687	ID=MsG0180000971.01.T01:three_prime_utr;Parent=MsG0180000971.01.T01

Gene Sequence

>MsG0180000971.01.T01

ATGATTTGCAGTCACAGTTCTTCCTTCATAATAACAATATCATTGCTAATACCTTTGCTTGGTAATGGCCAGAATATCCCATCCATTGGATGTCCTTTCAATCTATCTTGTACACATGATAACGAGAAGATTCTTGAAATAGCTGCTTATCCAGTCCCTATAAGACTCCTCATAAGTAACATTAACTACACATCGCAAGTATTACAAGCATCTGATCAGGAAGGCTGTCTTCCACGACTCTTACTTCAGTCCAATTTCTCTTCATCAATTTTTCCCTTTCGGATTATTGATGGTTCTGCGTTGCATCAGCAATTCAATGAATTGACCAATATTAGTTTCTTTGATTGTTCTTCACTAGGACAACGCCATCTGAGAAAGTACTACCAAATGTATGGAGTTGAGCAGCAGGACATGATTTCTTGTCCAATTTATATGGCAGCATTTGACGAAGATATGGTCGATATGAACCTTGTATCCTGTACCAAATTGTCTCAACAAGTTTCTCCCCTTATTCTTTCACATGAATGTGTATCTGGGATACAACAAAACTCAATCTCATTATCATGGTCGGAAACAAATCTCGACAAAGGATGCTTAGAATGTAAGCACAAATTAAAGAAGAAGATTAGTGTAGCCTCTGCAGGTGCAATCATTGGCTCAATTGTGCTTCTTCTTCTATTTGGTTTTATTTTTCAAATATATCGCTATTTTAAGATGAAAAGCGAAGATCATACAAGAATTGAAAACTTTTTGAAGGATTACAGAGATTTAAAGCCAACTAGATTCTCTTATGCAAACTTAAAGAGAATTACAAATAAATTTAAAGATAAGATAGGCGAAGGGACTCATGGAGCCGTTTATAAAGGTAAATTATCGAGCCAAATTCTAGTAGCCGTGAAAATTCTTAATAATACAGAGGAAGATGGGAAGGAATTTATAAATGAAGTGGGAACCATGGGCAAAATTCACCATCTAAATGTAGTTCGTTTGCTTGGCTTCTGCGCAGATGGTTTTCGTCGTGCTTTAGTCTATGACTTTTTTCCAAATGGTTCGTTGCAGAAATTCATTTCTCCCTCGTACAAAAAAGATGATTTCCTTGGATGGGATAAGTTGCAACAAATTGCTCTAGGCATAGCAAATGGCATTGAGTATCTCCACCAAGGTTGTGATCAAAGAATTCTACACTTTGACATCAATCCTAATAATGTCTTATTAGATGACAATTTCATTCCCAAAATTACAGATTTTGGTTTGGCTAAGATGTGTTCGAAAAATCAAAGTATTGTATCCATGACTGCGGCTAAGGGAACAATAGGCTACATGGCGCCTGAAGTTTTTTCTAGAAATTTTGGAAATGTCTCTTATAAGTCCGATATATACAGTTACGGTATGTTATTGCTGGAGATGGTTGGAGGAAGAAAGAATACAAAAACGACCGGTGGTGAAGAAAATATACAAGTGGAGTATCCAGACTGGATCCACAATTTGCTTGAAGGAGATATACAAATCCCTATTGATGAAGAGGGAGATTTTAGAATTCCAAAAAAATTGGCAACAGTGGGACTATGGTGTATCCAATGGCACCCTATGCATCGTCCGGCGATGAAATCTGTGATACAAATGCTTCAAGGAGAGGGGGGCAAGTTAAAAGTACCAACTAATCCTTTCGGACCAACAACTACAACTAATACAAATGCTAATATTGTTGCAGAACGCATGAATTTGGAGCTGGAGGTGATTGAAGAATTAGAATAA

Protein sequence

>MsG0180000971.01.T01

MICSHSSSFIITISLLIPLLGNGQNIPSIGCPFNLSCTHDNEKILEIAAYPVPIRLLISNINYTSQVLQASDQEGCLPRLLLQSNFSSSIFPFRIIDGSALHQQFNELTNISFFDCSSLGQRHLRKYYQMYGVEQQDMISCPIYMAAFDEDMVDMNLVSCTKLSQQVSPLILSHECVSGIQQNSISLSWSETNLDKGCLECKHKLKKKISVASAGAIIGSIVLLLLFGFIFQIYRYFKMKSEDHTRIENFLKDYRDLKPTRFSYANLKRITNKFKDKIGEGTHGAVYKGKLSSQILVAVKILNNTEEDGKEFINEVGTMGKIHHLNVVRLLGFCADGFRRALVYDFFPNGSLQKFISPSYKKDDFLGWDKLQQIALGIANGIEYLHQGCDQRILHFDINPNNVLLDDNFIPKITDFGLAKMCSKNQSIVSMTAAKGTIGYMAPEVFSRNFGNVSYKSDIYSYGMLLLEMVGGRKNTKTTGGEENIQVEYPDWIHNLLEGDIQIPIDEEGDFRIPKKLATVGLWCIQWHPMHRPAMKSVIQMLQGEGGKLKVPTNPFGPTTTTNTNANIVAERMNLELEVIEELE*