Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001420.01.T01 | XP_013466817.1 | 89.583 | 144 | 14 | 1 | 1 | 143 | 1 | 144 | 1.40E-82 | 257 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001420.01.T01 | P93324 | 46.575 | 146 | 65 | 4 | 1 | 143 | 13 | 148 | 1.11E-29 | 113 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001420.01.T01 | A0A072VHJ4 | 89.583 | 144 | 14 | 1 | 1 | 143 | 1 | 144 | 6.69e-83 | 257 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001393.01 | MsG0180001420.01 | 0.833678 | 4.647126e-56 | 5.390051e-53 |
| MsG0180001400.01 | MsG0180001420.01 | 0.802957 | 4.384839e-49 | 2.176436e-46 |
| MsG0180001403.01 | MsG0180001420.01 | 0.812884 | 3.387009e-51 | 2.178903e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001420.01.T01 | MTR_1g036490 | 89.583 | 144 | 14 | 1 | 1 | 143 | 1 | 144 | 1.70e-86 | 257 |
| MsG0180001420.01.T01 | MTR_1g036510 | 92.308 | 143 | 11 | 0 | 1 | 143 | 16 | 158 | 7.08e-86 | 252 |
| MsG0180001420.01.T01 | MTR_1g036510 | 92.308 | 143 | 11 | 0 | 1 | 143 | 16 | 158 | 2.02e-85 | 252 |
| MsG0180001420.01.T01 | MTR_1g036510 | 92.308 | 143 | 11 | 0 | 1 | 143 | 16 | 158 | 4.45e-84 | 251 |
| MsG0180001420.01.T01 | MTR_1g036460 | 84.722 | 144 | 21 | 1 | 1 | 143 | 1 | 144 | 1.18e-82 | 247 |
| MsG0180001420.01.T01 | MTR_1g050220 | 93.496 | 123 | 8 | 0 | 21 | 143 | 5 | 127 | 2.37e-79 | 236 |
| MsG0180001420.01.T01 | MTR_7g011900 | 48.299 | 147 | 65 | 6 | 3 | 143 | 5 | 146 | 1.59e-33 | 120 |
| MsG0180001420.01.T01 | MTR_7g012040 | 48.120 | 133 | 63 | 3 | 12 | 143 | 17 | 144 | 6.88e-32 | 116 |
| MsG0180001420.01.T01 | MTR_3g021440 | 50.000 | 126 | 59 | 2 | 11 | 135 | 16 | 138 | 3.63e-31 | 114 |
| MsG0180001420.01.T01 | MTR_2g055940 | 50.806 | 124 | 55 | 3 | 22 | 143 | 20 | 139 | 5.01e-31 | 114 |
| MsG0180001420.01.T01 | MTR_7g012020 | 47.761 | 134 | 63 | 4 | 12 | 143 | 17 | 145 | 6.17e-31 | 111 |
| MsG0180001420.01.T01 | MTR_1g096310 | 45.890 | 146 | 66 | 4 | 1 | 143 | 13 | 148 | 1.81e-30 | 112 |
| MsG0180001420.01.T01 | MTR_7g012020 | 47.761 | 134 | 63 | 4 | 12 | 143 | 17 | 145 | 2.13e-30 | 111 |
| MsG0180001420.01.T01 | MTR_7g012020 | 47.761 | 134 | 63 | 4 | 12 | 143 | 17 | 145 | 6.72e-30 | 111 |
| MsG0180001420.01.T01 | MTR_7g011990 | 44.444 | 135 | 63 | 4 | 12 | 143 | 18 | 143 | 1.18e-26 | 102 |
| MsG0180001420.01.T01 | MTR_3g021430 | 47.619 | 126 | 59 | 4 | 15 | 137 | 7 | 128 | 1.27e-24 | 97.1 |
| MsG0180001420.01.T01 | MTR_3g092900 | 37.879 | 132 | 79 | 1 | 3 | 134 | 2 | 130 | 1.28e-24 | 97.1 |
| MsG0180001420.01.T01 | MTR_7g012070 | 42.647 | 136 | 72 | 4 | 12 | 143 | 18 | 151 | 2.99e-23 | 93.6 |
| MsG0180001420.01.T01 | MTR_7g012090 | 43.609 | 133 | 70 | 4 | 16 | 143 | 19 | 151 | 6.11e-23 | 92.8 |
| MsG0180001420.01.T01 | MTR_8g068590 | 33.333 | 126 | 79 | 2 | 13 | 136 | 3 | 125 | 1.03e-18 | 77.4 |
| MsG0180001420.01.T01 | MTR_6g060290 | 38.806 | 134 | 61 | 4 | 12 | 143 | 18 | 132 | 1.83e-18 | 77.4 |
| MsG0180001420.01.T01 | MTR_8g068760 | 35.043 | 117 | 71 | 2 | 20 | 134 | 18 | 131 | 2.52e-17 | 75.9 |
| MsG0180001420.01.T01 | MTR_8g068740 | 33.088 | 136 | 85 | 3 | 1 | 134 | 13 | 144 | 1.11e-16 | 74.7 |
| MsG0180001420.01.T01 | MTR_0591s0010 | 34.167 | 120 | 74 | 2 | 18 | 135 | 29 | 145 | 1.70e-16 | 75.1 |
| MsG0180001420.01.T01 | MTR_8g068690 | 30.882 | 136 | 88 | 3 | 1 | 134 | 12 | 143 | 1.91e-16 | 73.9 |
| MsG0180001420.01.T01 | MTR_8g068730 | 35.294 | 119 | 72 | 2 | 18 | 134 | 29 | 144 | 2.56e-16 | 74.3 |
| MsG0180001420.01.T01 | MTR_7g118320 | 34.375 | 128 | 77 | 3 | 11 | 134 | 5 | 129 | 3.22e-16 | 74.3 |
| MsG0180001420.01.T01 | MTR_8g068690 | 30.882 | 136 | 88 | 3 | 1 | 134 | 12 | 143 | 3.59e-16 | 73.9 |
| MsG0180001420.01.T01 | MTR_8g068870 | 32.800 | 125 | 79 | 2 | 13 | 135 | 10 | 131 | 4.62e-16 | 73.6 |
| MsG0180001420.01.T01 | MTR_8g068750 | 34.454 | 119 | 73 | 2 | 18 | 134 | 29 | 144 | 1.07e-15 | 72.8 |
| MsG0180001420.01.T01 | MTR_7g118300 | 37.168 | 113 | 66 | 3 | 32 | 143 | 44 | 152 | 2.09e-15 | 72.0 |
| MsG0180001420.01.T01 | MTR_5g098170 | 36.975 | 119 | 69 | 3 | 13 | 128 | 9 | 124 | 2.38e-15 | 71.6 |
| MsG0180001420.01.T01 | MTR_8g068570 | 34.286 | 105 | 64 | 2 | 32 | 134 | 43 | 144 | 8.60e-15 | 70.1 |
| MsG0180001420.01.T01 | MTR_8g068600 | 36.842 | 95 | 55 | 2 | 43 | 135 | 28 | 119 | 1.79e-13 | 65.9 |
| MsG0180001420.01.T01 | MTR_6g078850 | 35.537 | 121 | 71 | 3 | 18 | 134 | 33 | 150 | 4.80e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001420.01.T01 | AT5G54160 | 36.364 | 132 | 79 | 1 | 3 | 134 | 2 | 128 | 4.49e-19 | 82.0 |
| MsG0180001420.01.T01 | AT1G33030 | 38.793 | 116 | 61 | 4 | 24 | 136 | 10 | 118 | 3.70e-15 | 70.5 |
| MsG0180001420.01.T01 | AT1G33030 | 38.793 | 116 | 61 | 4 | 24 | 136 | 10 | 118 | 4.89e-15 | 70.1 |
| MsG0180001420.01.T01 | AT1G33030 | 38.793 | 116 | 61 | 4 | 24 | 136 | 10 | 118 | 9.65e-15 | 70.1 |
| MsG0180001420.01.T01 | AT1G21130 | 39.394 | 132 | 70 | 5 | 1 | 128 | 9 | 134 | 1.08e-14 | 69.3 |
| MsG0180001420.01.T01 | AT1G21130 | 39.394 | 132 | 70 | 5 | 1 | 128 | 9 | 134 | 2.19e-14 | 68.9 |
| MsG0180001420.01.T01 | AT1G51990 | 30.973 | 113 | 76 | 1 | 18 | 130 | 13 | 123 | 1.10e-13 | 67.0 |
| MsG0180001420.01.T01 | AT1G51990 | 30.973 | 113 | 76 | 1 | 18 | 130 | 13 | 123 | 1.19e-13 | 67.0 |
| MsG0180001420.01.T01 | AT1G77530 | 44.086 | 93 | 43 | 4 | 33 | 119 | 42 | 131 | 5.94e-13 | 64.3 |
| MsG0180001420.01.T01 | AT1G77530 | 44.086 | 93 | 43 | 4 | 33 | 119 | 42 | 131 | 7.84e-13 | 64.7 |
Find 20 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATAAAGATGGAGGCTCTTT+AGG | 0.258602 | 1:-20843918 | MsG0180001420.01.T01:CDS |
| TCAAGCCTCTCAGCCAATTC+AGG | 0.269845 | 1:+20844059 | None:intergenic |
| GCCAATTCAGGGTGTTGATT+TGG | 0.300679 | 1:+20844071 | None:intergenic |
| TTCTTTCTTACCATCTTCAT+TGG | 0.332460 | 1:+20843983 | None:intergenic |
| ATAGCAGCATTTAATATTGC+AGG | 0.360567 | 1:+20844182 | None:intergenic |
| TCCAAATCAACACCCTGAAT+TGG | 0.385842 | 1:-20844072 | MsG0180001420.01.T01:CDS |
| ATCGTGGATATCATAATGTT+TGG | 0.434868 | 1:-20843875 | MsG0180001420.01.T01:CDS |
| GTTTATGCTCTCTCATCAAT+TGG | 0.442550 | 1:-20843957 | MsG0180001420.01.T01:CDS |
| TACTTTGCATTAGATAAAGA+TGG | 0.457536 | 1:-20843930 | MsG0180001420.01.T01:CDS |
| ACCATCTTCATTGGTACGAA+TGG | 0.502294 | 1:+20843992 | None:intergenic |
| AAACTGAGTTTGTTTGGTGT+TGG | 0.509463 | 1:+20844274 | None:intergenic |
| CAAGCCTCTCAGCCAATTCA+GGG | 0.537180 | 1:+20844060 | None:intergenic |
| TCCATTCGTACCAATGAAGA+TGG | 0.540221 | 1:-20843993 | MsG0180001420.01.T01:CDS |
| TGAACGTATGTTGACTGTGT+TGG | 0.557787 | 1:-20844039 | MsG0180001420.01.T01:CDS |
| AACATAGTAACAATGCAGAA+AGG | 0.583185 | 1:+20844219 | None:intergenic |
| CGATGGATCAATGTTGAGAG+TGG | 0.595896 | 1:+20843894 | None:intergenic |
| AACACCCTGAATTGGCTGAG+AGG | 0.629156 | 1:-20844064 | MsG0180001420.01.T01:CDS |
| TTTGCATTAGATAAAGATGG+AGG | 0.632452 | 1:-20843927 | MsG0180001420.01.T01:CDS |
| CTCTCAACATTGATCCATCG+TGG | 0.646255 | 1:-20843891 | MsG0180001420.01.T01:CDS |
| AAACATTATGATATCCACGA+TGG | 0.657170 | 1:+20843877 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CATTGTTACTATGTTTTATT+AGG | - | Chr1:20843942-20843961 | MsG0180001420.01.T01:CDS | 20.0% |
| ! | TACTTTGCATTAGATAAAGA+TGG | - | Chr1:20844223-20844242 | MsG0180001420.01.T01:CDS | 25.0% |
| AAACATTATGATATCCACGA+TGG | + | Chr1:20844279-20844298 | None:intergenic | 30.0% | |
| AACATAGTAACAATGCAGAA+AGG | + | Chr1:20843937-20843956 | None:intergenic | 30.0% | |
| ATAGCAGCATTTAATATTGC+AGG | + | Chr1:20843974-20843993 | None:intergenic | 30.0% | |
| ATCGTGGATATCATAATGTT+TGG | - | Chr1:20844278-20844297 | MsG0180001420.01.T01:CDS | 30.0% | |
| TTCTTTCTTACCATCTTCAT+TGG | + | Chr1:20844173-20844192 | None:intergenic | 30.0% | |
| TTTGCATTAGATAAAGATGG+AGG | - | Chr1:20844226-20844245 | MsG0180001420.01.T01:CDS | 30.0% | |
| GTTTATGCTCTCTCATCAAT+TGG | - | Chr1:20844196-20844215 | MsG0180001420.01.T01:CDS | 35.0% | |
| !! | AAACTGAGTTTGTTTGGTGT+TGG | + | Chr1:20843882-20843901 | None:intergenic | 35.0% |
| !!! | ATCAACTTTTTCAACCTCAG+GGG | + | Chr1:20843906-20843925 | None:intergenic | 35.0% |
| !!! | CATCAACTTTTTCAACCTCA+GGG | + | Chr1:20843907-20843926 | None:intergenic | 35.0% |
| !!! | TCATCAACTTTTTCAACCTC+AGG | + | Chr1:20843908-20843927 | None:intergenic | 35.0% |
| ACCATCTTCATTGGTACGAA+TGG | + | Chr1:20844164-20844183 | None:intergenic | 40.0% | |
| GATAAAGATGGAGGCTCTTT+AGG | - | Chr1:20844235-20844254 | MsG0180001420.01.T01:CDS | 40.0% | |
| TCCAAATCAACACCCTGAAT+TGG | - | Chr1:20844081-20844100 | MsG0180001420.01.T01:CDS | 40.0% | |
| TCCATTCGTACCAATGAAGA+TGG | - | Chr1:20844160-20844179 | MsG0180001420.01.T01:CDS | 40.0% | |
| ! | TGAACGTATGTTGACTGTGT+TGG | - | Chr1:20844114-20844133 | MsG0180001420.01.T01:CDS | 40.0% |
| !!! | TCAACTTTTTCAACCTCAGG+GGG | + | Chr1:20843905-20843924 | None:intergenic | 40.0% |
| CGATGGATCAATGTTGAGAG+TGG | + | Chr1:20844262-20844281 | None:intergenic | 45.0% | |
| CTCTCAACATTGATCCATCG+TGG | - | Chr1:20844262-20844281 | MsG0180001420.01.T01:CDS | 45.0% | |
| ! | AGGGGGAAACTGAGTTTGTT+TGG | + | Chr1:20843888-20843907 | None:intergenic | 45.0% |
| !! | GCCAATTCAGGGTGTTGATT+TGG | + | Chr1:20844085-20844104 | None:intergenic | 45.0% |
| AACACCCTGAATTGGCTGAG+AGG | - | Chr1:20844089-20844108 | MsG0180001420.01.T01:CDS | 50.0% | |
| ACAAACTCAGTTTCCCCCTG+AGG | - | Chr1:20843889-20843908 | MsG0180001420.01.T01:CDS | 50.0% | |
| CAAGCCTCTCAGCCAATTCA+GGG | + | Chr1:20844096-20844115 | None:intergenic | 50.0% | |
| TCAAGCCTCTCAGCCAATTC+AGG | + | Chr1:20844097-20844116 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 20843872 | 20844303 | 20843872 | ID=MsG0180001420.01;Name=MsG0180001420.01 |
| Chr1 | mRNA | 20843872 | 20844303 | 20843872 | ID=MsG0180001420.01.T01;Parent=MsG0180001420.01;Name=MsG0180001420.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|143 |
| Chr1 | exon | 20843872 | 20844303 | 20843872 | ID=MsG0180001420.01.T01:exon:26277;Parent=MsG0180001420.01.T01 |
| Chr1 | CDS | 20843872 | 20844303 | 20843872 | ID=MsG0180001420.01.T01:cds;Parent=MsG0180001420.01.T01 |
| Gene Sequence |
| Protein sequence |
