Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001614.01.T01 | XP_013466929.1 | 83.908 | 174 | 13 | 3 | 1 | 170 | 1 | 163 | 2.24E-96 | 286 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047831.01 | MsG0180001614.01 | -0.843819 | 1.108435e-58 | 1.755073e-55 |
| MsG0080047910.01 | MsG0180001614.01 | 0.827250 | 1.735340e-54 | 1.666236e-51 |
| MsG0080047945.01 | MsG0180001614.01 | 0.812060 | 5.126784e-51 | 3.226382e-48 |
| MsG0080047967.01 | MsG0180001614.01 | 0.822752 | 2.002653e-53 | 1.691352e-50 |
| MsG0080048036.01 | MsG0180001614.01 | -0.820988 | 5.126813e-53 | 4.119515e-50 |
| MsG0080048059.01 | MsG0180001614.01 | 0.873760 | 1.145714e-67 | 5.029819e-64 |
| MsG0080048202.01 | MsG0180001614.01 | -0.810464 | 1.137665e-50 | 6.862426e-48 |
| MsG0080048256.01 | MsG0180001614.01 | -0.831493 | 1.617576e-55 | 1.758239e-52 |
| MsG0080048486.01 | MsG0180001614.01 | -0.802194 | 6.298413e-49 | 3.066035e-46 |
| MsG0080048487.01 | MsG0180001614.01 | -0.802534 | 5.361134e-49 | 2.632521e-46 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001614.01.T01 | MTR_1g040145 | 83.908 | 174 | 13 | 3 | 1 | 170 | 1 | 163 | 2.71e-100 | 286 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001614.01.T01 | AT5G17560 | 74.194 | 93 | 24 | 0 | 73 | 165 | 84 | 176 | 2.09e-48 | 155 |
| MsG0180001614.01.T01 | AT5G17560 | 61.224 | 49 | 19 | 0 | 73 | 121 | 84 | 132 | 9.34e-16 | 70.5 |
| MsG0180001614.01.T01 | AT5G17560 | 63.830 | 47 | 17 | 0 | 73 | 119 | 84 | 130 | 1.14e-15 | 70.1 |
Find 59 sgRNAs with CRISPR-Local
Find 328 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACATAATGGAGAAGTAAA+TGG | 0.273966 | 1:-24123076 | None:intergenic |
| AAATTGTCAATTGTGTTGTT+TGG | 0.421046 | 1:-24122995 | None:intergenic |
| AACAAACCGCAGAACATTGC+TGG | 0.379497 | 1:-24123038 | None:intergenic |
| AACATAATGGAGAAGTAAAT+GGG | 0.420499 | 1:-24123075 | None:intergenic |
| AAGAGCTTTGCGGAGTAAAG+TGG | 0.561290 | 1:-24118375 | None:intergenic |
| AATAATTTCTCATCATGTTT+AGG | 0.269314 | 1:-24118506 | None:intergenic |
| AATCAGCCGTGAATAGGCAA+AGG | 0.635801 | 1:+24122695 | MsG0180001614.01.T01:CDS |
| ACGCTGTGTTGCTGCTGGGA+GGG | 0.493070 | 1:-24118304 | None:intergenic |
| ACTCCTGCTGAAGCAGCCGC+CGG | 0.495922 | 1:+24122787 | MsG0180001614.01.T01:CDS |
| ACTCTGAAGCTCCTCCCATA+TGG | 0.480068 | 1:-24122731 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAACAAAATCCCCCTAATT+TGG | + | Chr1:24119781-24119800 | MsG0180001614.01.T01:intron | 30.0% | |
| AAACACAGTTACAACTGTAT+CGG | + | Chr1:24118963-24118982 | MsG0180001614.01.T01:intron | 30.0% | |
| AAATAACATGACAATAAGGC+TGG | + | Chr1:24122396-24122415 | MsG0180001614.01.T01:intron | 30.0% | |
| AACCAACTAGATAATAAGCT+CGG | + | Chr1:24122359-24122378 | MsG0180001614.01.T01:intron | 30.0% | |
| AATCAAATCGTTTGTGAGTA+CGG | - | Chr1:24121967-24121986 | None:intergenic | 30.0% | |
| ACAAAACTTCAGACATTCAT+AGG | - | Chr1:24120907-24120926 | None:intergenic | 30.0% | |
| ACAGTCAACATAAAACTTTG+TGG | - | Chr1:24119951-24119970 | None:intergenic | 30.0% | |
| AGATCAATACATACTTGCTT+TGG | - | Chr1:24121898-24121917 | None:intergenic | 30.0% | |
| AGTATTCCAATTCACTTTCT+GGG | + | Chr1:24122950-24122969 | MsG0180001614.01.T01:three_prime_UTR | 30.0% | |
| ATAGTTCTCTCTCATAAAAG+TGG | + | Chr1:24121843-24121862 | MsG0180001614.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 24118185 | 24123135 | 24118185 | ID=MsG0180001614.01;Name=MsG0180001614.01 |
| Chr1 | mRNA | 24118185 | 24123135 | 24118185 | ID=MsG0180001614.01.T01;Parent=MsG0180001614.01;Name=MsG0180001614.01.T01;_AED=0.40;_eAED=0.40;_QI=156|1|1|1|1|1|3|319|170 |
| Chr1 | exon | 24118185 | 24118631 | 24118185 | ID=MsG0180001614.01.T01:exon:40656;Parent=MsG0180001614.01.T01 |
| Chr1 | exon | 24120592 | 24120659 | 24120592 | ID=MsG0180001614.01.T01:exon:40657;Parent=MsG0180001614.01.T01 |
| Chr1 | exon | 24122663 | 24123135 | 24122663 | ID=MsG0180001614.01.T01:exon:40658;Parent=MsG0180001614.01.T01 |
| Chr1 | five_prime_UTR | 24118185 | 24118340 | 24118185 | ID=MsG0180001614.01.T01:five_prime_utr;Parent=MsG0180001614.01.T01 |
| Chr1 | CDS | 24118341 | 24118631 | 24118341 | ID=MsG0180001614.01.T01:cds;Parent=MsG0180001614.01.T01 |
| Chr1 | CDS | 24120592 | 24120659 | 24120592 | ID=MsG0180001614.01.T01:cds;Parent=MsG0180001614.01.T01 |
| Chr1 | CDS | 24122663 | 24122816 | 24122663 | ID=MsG0180001614.01.T01:cds;Parent=MsG0180001614.01.T01 |
| Chr1 | three_prime_UTR | 24122817 | 24123135 | 24122817 | ID=MsG0180001614.01.T01:three_prime_utr;Parent=MsG0180001614.01.T01 |
| Gene Sequence |
>MsG0180001614.01.T01 ATGCGGGCACACGTTTTCTCACTTTCAGCACCCACCACTTTACTCCGCAAAGCTCTTCCCGTTTTGGTTCGACCTTACAGTAACAACAAATCCACCGTTCTCTTTCGTAATCATATCTCCTTCCTCAGATCCACTTCACAAACTACTACTCTCTTTCGCGATATTCCTAAACATGATGAGAAATTATTGTTCACCTCTGTTGCTTCTATTCGCAAGTTTACTTCACGCGCTACTAATGTAAACGATGCTGGTTCTATTGATTTGCCTCTCATTCAATCCATGCAGCAAAAGATAAAGGAACAACTCAATGCAGAAGCAGTCTCTGTAAAGGATGCTTATGGTGATGGACGGCATGTTAGCATTGATGTTATATCGACAGCCTTTGAGGGACAATCAGCCGTGAATAGGCAAAGGATGGTCTATAAAGCCATATGGGAGGAGCTTCAGAGTGCAGTTCATGCAGTTGATCAGATGACGACCAAAACTCCTGCTGAAGCAGCCGCCGGGAAATGA |
| Protein sequence |
>MsG0180001614.01.T01 MRAHVFSLSAPTTLLRKALPVLVRPYSNNKSTVLFRNHISFLRSTSQTTTLFRDIPKHDEKLLFTSVASIRKFTSRATNVNDAGSIDLPLIQSMQQKIKEQLNAEAVSVKDAYGDGRHVSIDVISTAFEGQSAVNRQRMVYKAIWEELQSAVHAVDQMTTKTPAEAAAGK* |
