AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001806.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001806.01.T01 MTR_1g043410 90.966 321 27 2 1 319 1 321 0.0 592
MsG0180001806.01.T01 MTR_1g074190 45.251 358 152 14 1 325 1 347 8.98e-78 241
MsG0180001806.01.T01 MTR_1g070140 39.198 324 164 12 18 322 29 338 3.93e-63 204
MsG0180001806.01.T01 MTR_7g096690 35.144 313 167 9 33 312 13 322 9.34e-52 174
MsG0180001806.01.T01 MTR_5g030010 30.627 271 133 10 26 272 50 289 1.55e-28 113
MsG0180001806.01.T01 MTR_8g066780 37.725 167 83 6 32 190 57 210 5.96e-28 111
MsG0180001806.01.T01 MTR_5g064890 33.014 209 111 9 19 214 44 236 2.81e-23 99.0
MsG0180001806.01.T01 MTR_4g093830 34.969 163 83 5 19 166 43 197 1.15e-21 94.4
MsG0180001806.01.T01 MTR_8g073120 28.221 163 97 4 9 170 4 147 1.87e-21 90.9
MsG0180001806.01.T01 MTR_3g099650 35.821 134 66 4 33 163 62 178 6.19e-21 92.8
MsG0180001806.01.T01 MTR_4g120270 51.316 76 32 1 91 166 79 149 1.40e-20 90.1
MsG0180001806.01.T01 MTR_1g082550 33.582 134 76 2 32 163 47 169 1.79e-20 91.3
MsG0180001806.01.T01 MTR_5g064990 50.000 76 38 0 91 166 113 188 2.09e-20 91.3
MsG0180001806.01.T01 MTR_7g113800 33.577 137 70 3 32 163 42 162 5.39e-20 89.7
MsG0180001806.01.T01 MTR_6g087150 33.121 157 89 6 17 168 21 166 9.21e-20 89.4
MsG0180001806.01.T01 MTR_8g089880 54.667 75 31 2 92 166 127 198 1.03e-19 87.8
MsG0180001806.01.T01 MTR_4g106680 32.168 143 84 4 32 167 42 178 1.08e-19 88.2
MsG0180001806.01.T01 MTR_7g116220 47.945 73 38 0 91 163 69 141 1.12e-19 85.5
MsG0180001806.01.T01 MTR_2g096350 47.297 74 38 1 93 166 108 180 8.56e-19 84.0
MsG0180001806.01.T01 MTR_5g034790 30.573 157 96 3 20 166 34 187 1.08e-18 86.3
MsG0180001806.01.T01 MTR_2g009310 50.667 75 34 1 92 166 135 206 1.10e-18 86.3
MsG0180001806.01.T01 MTR_7g117540 33.566 143 81 5 27 169 21 149 2.54e-18 84.0
MsG0180001806.01.T01 MTR_8g063660 50.000 72 33 1 92 163 68 136 3.66e-18 84.3
MsG0180001806.01.T01 MTR_3g086700 42.857 77 44 0 91 167 45 121 4.55e-18 81.3
MsG0180001806.01.T01 MTR_3g094000 25.161 155 103 4 12 166 23 164 6.56e-18 80.5
MsG0180001806.01.T01 MTR_3g110190 34.783 138 72 5 33 166 14 137 7.42e-18 83.6
MsG0180001806.01.T01 MTR_8g066740 31.757 148 88 4 19 163 11 148 8.37e-18 82.8
MsG0180001806.01.T01 MTR_7g076250 30.814 172 94 8 27 192 4 156 9.05e-18 80.5
MsG0180001806.01.T01 MTR_2g055790 35.514 107 57 2 66 166 45 145 1.01e-17 80.5
MsG0180001806.01.T01 MTR_8g093010 29.771 131 76 3 34 164 63 177 1.04e-17 80.9
MsG0180001806.01.T01 MTR_2g436100 42.045 88 48 2 79 166 80 164 1.06e-17 82.4
MsG0180001806.01.T01 MTR_5g064980 32.353 136 84 4 34 167 54 183 1.25e-17 83.2
MsG0180001806.01.T01 MTR_8g092930 26.289 194 122 6 11 192 30 214 1.29e-17 80.9
MsG0180001806.01.T01 MTR_3g086730 42.857 77 44 0 91 167 45 121 1.30e-17 80.9
MsG0180001806.01.T01 MTR_1g492830 32.593 135 83 3 29 163 4 130 1.43e-17 80.5
MsG0180001806.01.T01 MTR_8g092840 27.778 162 101 4 11 164 30 183 1.63e-17 80.5
MsG0180001806.01.T01 MTR_3g464780 42.169 83 46 2 86 166 95 177 1.98e-17 80.9
MsG0180001806.01.T01 MTR_1g070425 41.250 80 47 0 92 171 68 147 3.54e-17 78.6
MsG0180001806.01.T01 MTR_3g078470 29.412 204 128 8 41 232 20 219 3.83e-17 79.3
MsG0180001806.01.T01 MTR_8g071815 30.556 144 83 5 25 167 9 136 4.42e-17 78.2
MsG0180001806.01.T01 MTR_7g116180 41.250 80 47 0 92 171 67 146 4.50e-17 78.2
MsG0180001806.01.T01 MTR_5g024730 27.206 136 84 3 35 170 34 154 6.04e-17 78.6
MsG0180001806.01.T01 MTR_3g091620 33.846 130 65 4 92 203 101 227 8.83e-17 80.5
MsG0180001806.01.T01 MTR_6g007737 33.824 136 83 2 32 163 69 201 9.67e-17 80.5
MsG0180001806.01.T01 MTR_8g023400 45.455 77 38 2 92 168 92 164 1.28e-16 79.7
MsG0180001806.01.T01 MTR_5g076990 26.794 209 134 5 20 221 14 210 1.34e-16 79.3
MsG0180001806.01.T01 MTR_8g092985 28.105 153 95 5 34 182 62 203 1.58e-16 77.4
MsG0180001806.01.T01 MTR_2g090905 38.961 77 44 1 93 166 40 116 1.93e-16 75.1
MsG0180001806.01.T01 MTR_3g100400 42.667 75 40 2 92 166 130 201 2.46e-16 79.7
MsG0180001806.01.T01 MTR_6g055030 27.778 162 101 4 9 167 24 172 2.60e-16 78.6
MsG0180001806.01.T01 MTR_8g028465 45.833 72 34 1 92 163 90 156 6.40e-16 75.5
MsG0180001806.01.T01 MTR_5g082940 34.821 112 66 3 66 172 45 154 6.59e-16 74.7
MsG0180001806.01.T01 MTR_3g095560 25.000 168 106 4 7 164 22 179 7.11e-16 76.3
MsG0180001806.01.T01 MTR_2g090950 34.694 98 55 2 92 186 91 182 7.42e-16 76.3
MsG0180001806.01.T01 MTR_3g078450 44.444 72 39 1 92 163 76 146 8.30e-16 75.5
MsG0180001806.01.T01 MTR_5g066250 42.308 78 36 2 92 166 134 205 1.63e-15 77.4
MsG0180001806.01.T01 MTR_7g116200 43.836 73 37 1 91 163 67 135 1.83e-15 73.6
MsG0180001806.01.T01 MTR_5g026730 29.252 147 92 3 23 167 4 140 2.09e-15 73.6
MsG0180001806.01.T01 MTR_3g012550 31.250 144 80 4 28 163 18 150 2.09e-15 73.6
MsG0180001806.01.T01 MTR_7g079280 41.667 72 41 1 92 163 80 150 2.64e-15 73.2
MsG0180001806.01.T01 MTR_4g063070 35.789 95 55 2 74 163 285 378 4.29e-15 75.9
MsG0180001806.01.T01 MTR_3g071300 39.175 97 49 3 76 163 53 148 4.45e-15 72.4
MsG0180001806.01.T01 MTR_3g116000 33.333 114 65 2 64 170 44 153 4.88e-15 72.8
MsG0180001806.01.T01 MTR_1g026070 32.500 120 74 2 58 176 13 126 5.57e-15 71.2
MsG0180001806.01.T01 MTR_8g092960 37.333 75 45 1 90 164 101 173 5.80e-15 73.2
MsG0180001806.01.T01 MTR_8g009190 39.437 71 43 0 96 166 80 150 6.01e-15 74.7
MsG0180001806.01.T01 MTR_8g099495 41.935 93 46 3 76 163 300 389 6.67e-15 75.5
MsG0180001806.01.T01 MTR_2g044570 40.909 66 39 0 101 166 63 128 6.94e-15 74.3
MsG0180001806.01.T01 MTR_4g022410 35.354 99 62 1 72 170 66 162 7.27e-15 72.4
MsG0180001806.01.T01 MTR_5g009210 50.000 72 35 1 92 163 287 357 8.00e-15 74.7
MsG0180001806.01.T01 MTR_8g092970 27.222 180 104 5 23 192 44 206 8.37e-15 72.8
MsG0180001806.01.T01 MTR_5g026720 31.333 150 80 6 33 176 16 148 8.75e-15 71.2
MsG0180001806.01.T01 MTR_7g073200 31.933 119 75 3 46 163 36 149 1.04e-14 71.6
MsG0180001806.01.T01 MTR_5g009190 50.000 74 32 2 92 163 266 336 1.12e-14 74.3
MsG0180001806.01.T01 MTR_4g129420 41.333 75 36 2 92 163 117 186 2.08e-14 74.3
MsG0180001806.01.T01 MTR_7g116210 42.466 73 38 1 91 163 9 77 2.17e-14 68.9
MsG0180001806.01.T01 MTR_5g009200 50.000 74 32 2 92 163 322 392 2.89e-14 73.2
MsG0180001806.01.T01 MTR_1g090480 38.356 73 44 1 92 163 72 144 4.93e-14 69.3
MsG0180001806.01.T01 MTR_7g079290 41.026 78 35 2 92 163 125 197 6.31e-14 70.1
MsG0180001806.01.T01 MTR_5g092790 36.709 79 43 2 91 166 156 230 6.86e-14 70.5
MsG0180001806.01.T01 MTR_8g092990 36.842 95 54 3 102 192 124 216 7.02e-14 70.5
MsG0180001806.01.T01 MTR_5g071930 41.096 73 37 2 91 163 65 131 7.99e-14 69.7
MsG0180001806.01.T01 MTR_1g056350 37.500 72 45 0 92 163 74 145 1.01e-13 68.6
MsG0180001806.01.T01 MTR_8g032690 53.488 43 20 0 121 163 19 61 1.83e-13 66.2
MsG0180001806.01.T01 MTR_4g030070 34.615 104 59 4 60 163 58 152 1.93e-13 68.9
MsG0180001806.01.T01 MTR_4g056590 34.694 98 51 3 66 163 81 165 2.47e-13 68.2
MsG0180001806.01.T01 MTR_7g108855 36.264 91 57 1 76 166 50 139 4.66e-13 67.4
MsG0180001806.01.T01 MTR_3g114500 30.909 110 69 2 58 166 13 116 6.62e-13 65.1
MsG0180001806.01.T01 MTR_5g092750 36.709 79 43 2 91 166 156 230 6.72e-13 67.8
MsG0180001806.01.T01 MTR_5g022920 36.842 76 47 1 91 166 160 234 1.30e-12 67.0
MsG0180001806.01.T01 MTR_8g074900 35.632 87 48 2 78 164 145 223 2.20e-12 65.9
MsG0180001806.01.T01 MTR_8g019710 38.667 75 38 1 109 183 42 108 3.71e-12 63.5
MsG0180001806.01.T01 MTR_3g081030 40.244 82 43 2 84 163 84 161 4.74e-12 65.1
MsG0180001806.01.T01 MTR_7g117350 37.805 82 48 1 85 163 283 364 6.32e-12 66.2
MsG0180001806.01.T01 MTR_7g117350 37.805 82 48 1 85 163 275 356 6.84e-12 66.2
MsG0180001806.01.T01 MTR_1g090460 39.394 66 39 1 99 163 72 137 7.17e-12 63.2
MsG0180001806.01.T01 MTR_1g100737 32.051 78 51 1 91 166 91 168 8.52e-12 63.9
MsG0180001806.01.T01 MTR_2g041200 26.207 145 94 2 26 167 21 155 9.33e-12 63.2
MsG0180001806.01.T01 MTR_8g099515 58.140 43 18 0 121 163 337 379 1.02e-11 65.9
MsG0180001806.01.T01 MTR_3g078520 31.915 94 58 2 92 181 76 167 1.02e-11 62.8
MsG0180001806.01.T01 MTR_7g059360 41.935 62 34 1 106 167 145 204 1.36e-11 63.9
MsG0180001806.01.T01 MTR_8g027980 37.838 74 42 2 95 166 93 164 1.64e-11 63.9
MsG0180001806.01.T01 MTR_2g035800 22.917 144 97 2 35 178 30 159 1.72e-11 62.0
MsG0180001806.01.T01 MTR_1g114240 35.366 82 50 2 88 169 257 335 2.83e-11 63.9
MsG0180001806.01.T01 MTR_1g114240 35.366 82 50 2 88 169 251 329 2.85e-11 63.9
MsG0180001806.01.T01 MTR_2g079320 51.111 45 22 0 119 163 157 201 2.95e-11 62.4
MsG0180001806.01.T01 MTR_3g078540 29.630 81 57 0 92 172 50 130 4.36e-11 60.8
MsG0180001806.01.T01 MTR_3g078120 34.783 69 44 1 98 166 63 130 5.75e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001806.01.T01 AT1G04360 41.547 349 151 13 27 326 37 381 1.41e-65 211
MsG0180001806.01.T01 AT5G43420 40.789 304 113 12 27 280 30 316 9.54e-61 199
MsG0180001806.01.T01 AT3G03550 28.017 232 124 5 32 238 58 271 2.89e-28 112
MsG0180001806.01.T01 AT4G17905 34.254 181 106 4 32 205 60 234 3.78e-28 111
MsG0180001806.01.T01 AT4G17905 34.254 181 106 4 32 205 86 260 8.51e-28 111
MsG0180001806.01.T01 AT5G17600 35.338 133 83 1 35 167 59 188 1.12e-24 102
MsG0180001806.01.T01 AT4G28890 32.961 179 94 6 9 174 11 176 3.22e-22 97.1
MsG0180001806.01.T01 AT1G23980 35.971 139 79 2 32 163 51 186 1.02e-21 94.7
MsG0180001806.01.T01 AT1G72220 47.826 92 39 2 92 183 151 233 4.71e-21 93.6
MsG0180001806.01.T01 AT3G05200 28.931 159 96 4 14 167 28 174 1.47e-20 91.7
MsG0180001806.01.T01 AT2G20030 33.136 169 98 6 12 174 18 177 2.05e-20 91.3
MsG0180001806.01.T01 AT2G34990 36.000 125 77 3 40 163 16 138 2.41e-20 90.1
MsG0180001806.01.T01 AT2G34990 36.000 125 77 3 40 163 38 160 3.27e-20 90.1
MsG0180001806.01.T01 AT3G16720 32.895 152 78 3 91 233 93 229 3.58e-20 89.4
MsG0180001806.01.T01 AT3G18773 30.061 163 102 4 2 163 21 172 5.22e-20 87.4
MsG0180001806.01.T01 AT4G09100 45.455 88 46 2 80 166 44 130 5.54e-20 84.7
MsG0180001806.01.T01 AT1G35330 34.161 161 91 5 9 163 20 171 6.44e-20 89.4
MsG0180001806.01.T01 AT4G40070 32.500 160 98 3 9 167 20 170 2.11e-19 87.8
MsG0180001806.01.T01 AT2G35000 32.639 144 89 3 30 171 48 185 2.65e-19 88.2
MsG0180001806.01.T01 AT4G09110 26.538 260 136 10 29 275 44 261 7.36e-19 85.9
MsG0180001806.01.T01 AT4G09120 48.750 80 37 2 91 168 93 170 7.55e-19 86.3
MsG0180001806.01.T01 AT5G10380 50.000 82 34 3 92 171 109 185 8.93e-19 85.5
MsG0180001806.01.T01 AT1G72200 30.597 134 83 3 32 163 61 186 1.27e-18 86.3
MsG0180001806.01.T01 AT2G27940 34.043 141 85 3 26 163 47 182 4.04e-18 82.4
MsG0180001806.01.T01 AT5G40250 32.414 145 83 4 32 163 43 185 4.23e-18 84.7
MsG0180001806.01.T01 AT1G76410 30.952 126 84 2 46 170 30 153 5.04e-18 80.9
MsG0180001806.01.T01 AT3G48030 31.902 163 81 6 22 169 30 177 6.02e-18 82.8
MsG0180001806.01.T01 AT4G17920 28.889 135 96 0 32 166 21 155 6.06e-18 83.2
MsG0180001806.01.T01 AT3G48030 31.902 163 81 6 22 169 108 255 8.79e-18 83.2
MsG0180001806.01.T01 AT1G49220 34.426 122 74 3 45 163 59 177 1.03e-17 81.6
MsG0180001806.01.T01 AT5G27420 31.206 141 89 2 27 167 38 170 1.20e-17 83.2
MsG0180001806.01.T01 AT2G17450 26.946 167 101 4 17 179 11 160 1.42e-17 79.7
MsG0180001806.01.T01 AT5G57750 32.353 136 83 5 32 163 34 164 2.16e-17 79.7
MsG0180001806.01.T01 AT5G05810 32.143 140 88 4 31 166 55 191 3.25e-17 82.0
MsG0180001806.01.T01 AT5G46650 31.081 148 92 3 25 166 17 160 3.64e-17 80.9
MsG0180001806.01.T01 AT1G20823 27.815 151 102 4 20 170 17 160 5.79e-17 78.6
MsG0180001806.01.T01 AT4G15975 30.323 155 72 5 92 243 51 172 7.53e-17 79.0
MsG0180001806.01.T01 AT2G46160 42.857 77 39 1 92 163 104 180 7.88e-17 78.6
MsG0180001806.01.T01 AT2G46160 42.857 77 39 1 92 163 104 180 7.88e-17 78.6
MsG0180001806.01.T01 AT4G30400 45.833 72 37 1 92 163 107 176 7.92e-17 81.3
MsG0180001806.01.T01 AT2G42360 29.412 170 91 4 34 203 36 176 8.71e-17 79.0
MsG0180001806.01.T01 AT4G09130 43.210 81 42 2 90 168 89 167 1.20e-16 80.1
MsG0180001806.01.T01 AT1G49210 30.769 130 80 3 34 163 58 177 1.40e-16 77.8
MsG0180001806.01.T01 AT3G14320 29.286 140 88 5 33 170 7 137 1.62e-16 77.4
MsG0180001806.01.T01 AT4G33565 45.333 75 38 1 92 166 191 262 1.65e-16 79.7
MsG0180001806.01.T01 AT2G47560 39.286 84 50 1 83 166 71 153 3.24e-16 77.0
MsG0180001806.01.T01 AT1G22500 45.833 72 39 0 92 163 89 160 3.30e-16 79.0
MsG0180001806.01.T01 AT2G35910 44.444 72 37 1 92 163 121 189 4.59e-16 76.3
MsG0180001806.01.T01 AT2G18650 45.205 73 36 2 92 163 99 168 5.01e-16 79.0
MsG0180001806.01.T01 AT3G61550 41.975 81 37 2 92 163 99 178 5.22e-16 76.3
MsG0180001806.01.T01 AT3G62690 40.000 80 45 1 93 172 88 164 6.88e-16 76.6
MsG0180001806.01.T01 AT5G01880 37.931 87 49 2 81 166 68 150 8.67e-16 74.3
MsG0180001806.01.T01 AT1G49200 33.058 121 77 2 45 163 60 178 8.88e-16 75.9
MsG0180001806.01.T01 AT1G53820 37.374 99 52 2 68 166 77 165 2.47e-15 75.9
MsG0180001806.01.T01 AT3G18930 37.778 90 48 2 84 166 113 201 3.39e-15 76.3
MsG0180001806.01.T01 AT3G18930 37.778 90 48 2 84 166 113 201 3.39e-15 76.3
MsG0180001806.01.T01 AT3G18930 37.778 90 48 2 84 166 113 201 3.39e-15 76.3
MsG0180001806.01.T01 AT5G05280 27.068 133 82 3 34 166 41 158 3.83e-15 72.8
MsG0180001806.01.T01 AT2G42350 35.345 116 56 4 91 197 74 179 4.03e-15 73.6
MsG0180001806.01.T01 AT5G06490 36.082 97 54 3 71 163 79 171 5.56e-15 72.8
MsG0180001806.01.T01 AT4G35840 35.227 88 57 0 79 166 149 236 5.99e-15 73.6
MsG0180001806.01.T01 AT2G34000 34.314 102 66 1 64 164 33 134 7.01e-15 71.6
MsG0180001806.01.T01 AT2G46493 44.000 75 39 2 90 163 108 180 8.58e-15 72.0
MsG0180001806.01.T01 AT3G10910 24.118 170 96 5 34 195 33 177 1.08e-14 71.6
MsG0180001806.01.T01 AT5G07040 29.801 151 79 4 28 170 12 143 1.32e-14 70.9
MsG0180001806.01.T01 AT4G10160 29.907 107 59 2 86 192 66 156 1.55e-14 72.4
MsG0180001806.01.T01 AT1G53010 31.818 154 80 7 33 164 23 173 1.80e-14 70.9
MsG0180001806.01.T01 AT3G60220 42.029 69 40 0 95 163 91 159 2.11e-14 73.6
MsG0180001806.01.T01 AT1G32361 36.905 84 48 2 92 175 130 208 2.14e-14 73.2
MsG0180001806.01.T01 AT2G35420 27.465 142 79 5 30 163 20 145 2.39e-14 72.0
MsG0180001806.01.T01 AT4G10150 29.412 136 66 5 60 192 62 170 2.44e-14 72.0
MsG0180001806.01.T01 AT3G19140 30.769 143 81 6 43 184 13 138 4.14e-14 68.9
MsG0180001806.01.T01 AT5G53110 45.833 72 38 1 92 163 302 372 4.75e-14 72.8
MsG0180001806.01.T01 AT1G49230 29.545 132 86 2 40 171 57 181 5.28e-14 70.5
MsG0180001806.01.T01 AT1G72310 32.990 97 54 2 70 166 87 172 5.63e-14 72.0
MsG0180001806.01.T01 AT1G33480 33.708 89 55 1 85 173 68 152 1.59e-13 70.1
MsG0180001806.01.T01 AT1G80400 37.000 100 41 3 88 166 301 399 2.98e-13 70.5
MsG0180001806.01.T01 AT5G66070 38.462 78 43 2 87 164 147 219 4.21e-13 68.2
MsG0180001806.01.T01 AT5G66070 38.462 78 43 2 87 164 147 219 4.21e-13 68.2
MsG0180001806.01.T01 AT4G35480 51.064 47 23 0 118 164 110 156 5.60e-13 67.4
MsG0180001806.01.T01 AT3G20395 30.435 115 67 3 53 167 116 217 6.08e-13 67.8
MsG0180001806.01.T01 AT5G66070 35.227 88 52 2 77 164 161 243 7.06e-13 67.8
MsG0180001806.01.T01 AT5G58580 39.024 82 48 1 85 166 104 183 8.89e-13 68.2
MsG0180001806.01.T01 AT2G17730 32.500 80 54 0 87 166 162 241 1.58e-12 66.6
MsG0180001806.01.T01 AT2G46494 44.595 74 38 2 91 163 287 358 2.11e-12 67.8
MsG0180001806.01.T01 AT2G46495 38.750 80 49 0 92 171 263 342 2.20e-12 67.4
MsG0180001806.01.T01 AT2G46495 38.750 80 49 0 92 171 291 370 2.80e-12 67.4
MsG0180001806.01.T01 AT2G18670 32.500 80 52 1 92 171 83 160 5.22e-12 64.3
MsG0180001806.01.T01 AT1G28040 29.167 168 93 6 3 163 147 295 5.37e-12 65.9
MsG0180001806.01.T01 AT5G47610 34.177 79 51 1 89 166 74 152 8.62e-12 63.2
MsG0180001806.01.T01 AT2G25410 35.000 80 52 0 92 171 298 377 2.06e-11 64.7
MsG0180001806.01.T01 AT2G37580 36.765 68 43 0 107 174 128 195 6.43e-11 62.0
MsG0180001806.01.T01 AT4G24015 47.368 57 29 1 114 169 97 153 7.16e-11 60.8
MsG0180001806.01.T01 AT5G42200 35.065 77 47 1 91 167 77 150 8.14e-11 60.5

Find 43 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCAGAATCAGGATGATTT+TGG 0.187559 1:-27334289 None:intergenic
GAGAAAGACTATGGTGAATT+AGG 0.240210 1:+27334551 MsG0180001806.01.T01:CDS
TGAAATAAATGGTTGCAATT+TGG 0.290797 1:-27334638 None:intergenic
GGAAAATTTGTACCAGAATC+AGG 0.300460 1:-27334299 None:intergenic
CACAATCAATGTGAAATAAA+TGG 0.300719 1:-27334649 None:intergenic
ATTGAGAAAGACCTTCTAAT+TGG 0.305044 1:-27335016 None:intergenic
GGCAACATCTTTCTTGCTAA+TGG 0.314698 1:+27334349 MsG0180001806.01.T01:CDS
CCTACTACAATGATTACTAT+TGG 0.329488 1:-27334320 None:intergenic
TTCTAGTTCAACCAATTAGA+AGG 0.338589 1:+27335005 MsG0180001806.01.T01:CDS
ACATTGATTGTGTTGATGTT+TGG 0.348549 1:+27334660 MsG0180001806.01.T01:CDS
ATCCAAAATCATCCTGATTC+TGG 0.350355 1:+27334287 MsG0180001806.01.T01:CDS
CCAATAGTAATCATTGTAGT+AGG 0.351049 1:+27334320 MsG0180001806.01.T01:CDS
CAACCTAGTTTGCATGTTCA+TGG 0.351504 1:+27335067 MsG0180001806.01.T01:CDS
AACAATATTGATCTTGTTAG+AGG 0.364558 1:+27334410 MsG0180001806.01.T01:CDS
AGATGAAAGAGGTAAAGAAT+TGG 0.372927 1:+27334892 MsG0180001806.01.T01:CDS
TTTCAAGGGTCCAAATGAAT+TGG 0.374461 1:-27335187 None:intergenic
TGATCAAATGCTGCTTCAAT+TGG 0.375168 1:+27334387 MsG0180001806.01.T01:CDS
AAGGAAAAGAATGATCTCTT+AGG 0.393134 1:-27335130 None:intergenic
TACAAAGAAGAGAAAGACTA+TGG 0.419059 1:+27334542 MsG0180001806.01.T01:CDS
TGCAAATAAGGTGAAGGTCT+TGG 0.431537 1:-27334764 None:intergenic
TCTTCTTTGTAATGAATCAC+TGG 0.437626 1:-27334530 None:intergenic
CAAAATGTTGAAAACATCAT+TGG 0.439598 1:+27334791 MsG0180001806.01.T01:CDS
AATTAGAAGGTCTTTCTCAA+TGG 0.453667 1:+27335018 MsG0180001806.01.T01:CDS
GTTAGTTCCATTGAAGTTTG+TGG 0.456288 1:+27335091 MsG0180001806.01.T01:CDS
ACTATCACCACAAACTTCAA+TGG 0.458585 1:-27335098 None:intergenic
AACCTAGTTTGCATGTTCAT+GGG 0.461073 1:+27335068 MsG0180001806.01.T01:CDS
GATTTCTTGTGTGAATCTCT+TGG 0.478795 1:-27334920 None:intergenic
TGAAAGAGGTAAAGAATTGG+AGG 0.511082 1:+27334895 MsG0180001806.01.T01:CDS
GAGTGACAACATGCTCAACT+TGG 0.515758 1:-27334742 None:intergenic
AATCATTGTAGTAGGAATGA+TGG 0.520403 1:+27334328 MsG0180001806.01.T01:CDS
TCATAGAGGATTAGAACAAT+CGG 0.536092 1:+27334493 MsG0180001806.01.T01:CDS
ACCCCATGAACATGCAAACT+AGG 0.537002 1:-27335070 None:intergenic
TGTATTAAAGACAAAGATGA+TGG 0.537948 1:+27334980 MsG0180001806.01.T01:CDS
TGGCTTTGCAAATAAGGTGA+AGG 0.542682 1:-27334770 None:intergenic
AATAATGAAAATGACCATGA+AGG 0.566631 1:+27334848 MsG0180001806.01.T01:CDS
ACCTAGTTTGCATGTTCATG+GGG 0.568859 1:+27335069 MsG0180001806.01.T01:CDS
CATGAAGGAAGAGATGAAAG+AGG 0.578510 1:+27334881 MsG0180001806.01.T01:CDS
AATGTTGAAAACATCATTGG+TGG 0.585029 1:+27334794 MsG0180001806.01.T01:CDS
TGATCTACTTCCATGTCCAA+AGG 0.586042 1:-27335149 None:intergenic
GAAGGTCAAAACTTGCATGA+AGG 0.612954 1:+27334866 MsG0180001806.01.T01:CDS
TCGATGACATCGGATCATAG+AGG 0.645344 1:+27334479 MsG0180001806.01.T01:CDS
ATCTACATACTCGATGACAT+CGG 0.664687 1:+27334469 MsG0180001806.01.T01:CDS
GAAGCAGCATTTGATCACAA+AGG 0.695774 1:-27334381 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGATTTTGTTGTAATTGATT+TGG + Chr1:27334823-27334842 MsG0180001806.01.T01:CDS 20.0%
!!! TATTGTTTTACCAATTCATT+TGG + Chr1:27335177-27335196 MsG0180001806.01.T01:CDS 20.0%
! AACAATATTGATCTTGTTAG+AGG + Chr1:27334410-27334429 MsG0180001806.01.T01:CDS 25.0%
! AATAATGAAAATGACCATGA+AGG + Chr1:27334848-27334867 MsG0180001806.01.T01:CDS 25.0%
! CAAAATGTTGAAAACATCAT+TGG + Chr1:27334791-27334810 MsG0180001806.01.T01:CDS 25.0%
! CACAATCAATGTGAAATAAA+TGG - Chr1:27334652-27334671 None:intergenic 25.0%
! TGAAATAAATGGTTGCAATT+TGG - Chr1:27334641-27334660 None:intergenic 25.0%
! TGTATTAAAGACAAAGATGA+TGG + Chr1:27334980-27334999 MsG0180001806.01.T01:CDS 25.0%
!! CAATGATGTTTTCAACATTT+TGG - Chr1:27334793-27334812 None:intergenic 25.0%
AAGGAAAAGAATGATCTCTT+AGG - Chr1:27335133-27335152 None:intergenic 30.0%
AATCATTGTAGTAGGAATGA+TGG + Chr1:27334328-27334347 MsG0180001806.01.T01:CDS 30.0%
AATGTTGAAAACATCATTGG+TGG + Chr1:27334794-27334813 MsG0180001806.01.T01:CDS 30.0%
AATTAGAAGGTCTTTCTCAA+TGG + Chr1:27335018-27335037 MsG0180001806.01.T01:CDS 30.0%
AGATGAAAGAGGTAAAGAAT+TGG + Chr1:27334892-27334911 MsG0180001806.01.T01:CDS 30.0%
ATTGAGAAAGACCTTCTAAT+TGG - Chr1:27335019-27335038 None:intergenic 30.0%
CCAATAGTAATCATTGTAGT+AGG + Chr1:27334320-27334339 MsG0180001806.01.T01:CDS 30.0%
CCTACTACAATGATTACTAT+TGG - Chr1:27334323-27334342 None:intergenic 30.0%
TACAAAGAAGAGAAAGACTA+TGG + Chr1:27334542-27334561 MsG0180001806.01.T01:CDS 30.0%
TCATAGAGGATTAGAACAAT+CGG + Chr1:27334493-27334512 MsG0180001806.01.T01:CDS 30.0%
TCTTCTTTGTAATGAATCAC+TGG - Chr1:27334533-27334552 None:intergenic 30.0%
! AAGAGATCATTCTTTTCCTT+TGG + Chr1:27335133-27335152 MsG0180001806.01.T01:CDS 30.0%
! ACATTGATTGTGTTGATGTT+TGG + Chr1:27334660-27334679 MsG0180001806.01.T01:CDS 30.0%
! GAAACTTTTCTTCTACAAAG+TGG - Chr1:27334707-27334726 None:intergenic 30.0%
!! TTCTAGTTCAACCAATTAGA+AGG + Chr1:27335005-27335024 MsG0180001806.01.T01:CDS 30.0%
!!! ACATTTTGGCTTTGCAAATA+AGG - Chr1:27334779-27334798 None:intergenic 30.0%
ACTATCACCACAAACTTCAA+TGG - Chr1:27335101-27335120 None:intergenic 35.0%
ATCCAAAATCATCCTGATTC+TGG + Chr1:27334287-27334306 MsG0180001806.01.T01:CDS 35.0%
ATCTACATACTCGATGACAT+CGG + Chr1:27334469-27334488 MsG0180001806.01.T01:CDS 35.0%
GAGAAAGACTATGGTGAATT+AGG + Chr1:27334551-27334570 MsG0180001806.01.T01:CDS 35.0%
GATTTCTTGTGTGAATCTCT+TGG - Chr1:27334923-27334942 None:intergenic 35.0%
GGAAAATTTGTACCAGAATC+AGG - Chr1:27334302-27334321 None:intergenic 35.0%
GTTAGTTCCATTGAAGTTTG+TGG + Chr1:27335091-27335110 MsG0180001806.01.T01:CDS 35.0%
TGAAAGAGGTAAAGAATTGG+AGG + Chr1:27334895-27334914 MsG0180001806.01.T01:CDS 35.0%
! AACCTAGTTTGCATGTTCAT+GGG + Chr1:27335068-27335087 MsG0180001806.01.T01:CDS 35.0%
! GAATCAGGATGATTTTGGAT+AGG - Chr1:27334287-27334306 None:intergenic 35.0%
! TACCAGAATCAGGATGATTT+TGG - Chr1:27334292-27334311 None:intergenic 35.0%
! TCATTCTTTTCCTTTGGACA+TGG + Chr1:27335139-27335158 MsG0180001806.01.T01:CDS 35.0%
! TGATCAAATGCTGCTTCAAT+TGG + Chr1:27334387-27334406 MsG0180001806.01.T01:CDS 35.0%
!! CTTCAAAAAGTGTCTAGCAT+GGG + Chr1:27334950-27334969 MsG0180001806.01.T01:CDS 35.0%
!! TTCAAAAAGTGTCTAGCATG+GGG + Chr1:27334951-27334970 MsG0180001806.01.T01:CDS 35.0%
CATGAAGGAAGAGATGAAAG+AGG + Chr1:27334881-27334900 MsG0180001806.01.T01:CDS 40.0%
GAAGCAGCATTTGATCACAA+AGG - Chr1:27334384-27334403 None:intergenic 40.0%
GAAGGTCAAAACTTGCATGA+AGG + Chr1:27334866-27334885 MsG0180001806.01.T01:CDS 40.0%
GGCAACATCTTTCTTGCTAA+TGG + Chr1:27334349-27334368 MsG0180001806.01.T01:CDS 40.0%
TGATCTACTTCCATGTCCAA+AGG - Chr1:27335152-27335171 None:intergenic 40.0%
TGCAAATAAGGTGAAGGTCT+TGG - Chr1:27334767-27334786 None:intergenic 40.0%
! ACCTAGTTTGCATGTTCATG+GGG + Chr1:27335069-27335088 MsG0180001806.01.T01:CDS 40.0%
! CAACCTAGTTTGCATGTTCA+TGG + Chr1:27335067-27335086 MsG0180001806.01.T01:CDS 40.0%
! TCAGGATGATTTTGGATAGG+TGG - Chr1:27334284-27334303 None:intergenic 40.0%
! TGGCTTTGCAAATAAGGTGA+AGG - Chr1:27334773-27334792 None:intergenic 40.0%
!! CATGCAAGTTTTGACCTTCA+TGG - Chr1:27334865-27334884 None:intergenic 40.0%
!! GCTTCAAAAAGTGTCTAGCA+TGG + Chr1:27334949-27334968 MsG0180001806.01.T01:CDS 40.0%
!! TCAAAAAGTGTCTAGCATGG+GGG + Chr1:27334952-27334971 MsG0180001806.01.T01:CDS 40.0%
ACCCCATGAACATGCAAACT+AGG - Chr1:27335073-27335092 None:intergenic 45.0%
GAGTGACAACATGCTCAACT+TGG - Chr1:27334745-27334764 None:intergenic 45.0%
TCGATGACATCGGATCATAG+AGG + Chr1:27334479-27334498 MsG0180001806.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr1 gene 27334227 27335207 27334227 ID=MsG0180001806.01;Name=MsG0180001806.01
Chr1 mRNA 27334227 27335207 27334227 ID=MsG0180001806.01.T01;Parent=MsG0180001806.01;Name=MsG0180001806.01.T01;_AED=0.09;_eAED=0.09;_QI=0|-1|0|1|-1|1|1|0|326
Chr1 exon 27334227 27335207 27334227 ID=MsG0180001806.01.T01:exon:34823;Parent=MsG0180001806.01.T01
Chr1 CDS 27334227 27335207 27334227 ID=MsG0180001806.01.T01:cds;Parent=MsG0180001806.01.T01
Gene Sequence

>MsG0180001806.01.T01

ATGAATTTTGTTAACAAACAACACTTGATCATCAATCATGTGTCAGAAGCTCATCCACCTATCCAAAATCATCCTGATTCTGGTACAAATTTTCCAATAGTAATCATTGTAGTAGGAATGATGGCAACATCTTTCTTGCTAATGGCATACTACACCTTTGTGATCAAATGCTGCTTCAATTGGAACAATATTGATCTTGTTAGAGGAAGACGTTTTTCTTTTTCAAGACAACATGAAGAACAATCTACATACTCGATGACATCGGATCATAGAGGATTAGAACAATCGGTGATTAATTCAATTCCAGTGATTCATTACAAAGAAGAGAAAGACTATGGTGAATTAGGAATCTCTAGTGAATGTGCTTTTTGTTTGAGTGAGTTTCAAGAAGATGAGATACTAAGAGTTATACCAAATTGCAACCATTTATTTCACATTGATTGTGTTGATGTTTGGCTTCAAAACAATGCAAATTGTCCACTTTGTAGAAGAAAAGTTTCAATGACAAGAGAAATCCAAGTTGAGCATGTTGTCACTCCAAGACCTTCACCTTATTTGCAAAGCCAAAATGTTGAAAACATCATTGGTGGTTGTGAAGATTTTGTTGTAATTGATTTGGATAATAATGAAAATGACCATGAAGGTCAAAACTTGCATGAAGGAAGAGATGAAAGAGGTAAAGAATTGGAGGTACCAAGAGATTCACACAAGAAATCAATTAAGCTTCAAAAAGTGTCTAGCATGGGGGATGAGTGTATTAAAGACAAAGATGATGGCTTTCTAGTTCAACCAATTAGAAGGTCTTTCTCAATGGACTTAGCAATACATAAATCTTTATATCAACCTAGTTTGCATGTTCATGGGGTTAGTTCCATTGAAGTTTGTGGTGATAGTAGTAATAGACCTAAGAGATCATTCTTTTCCTTTGGACATGGAAGTAGATCAAGAAGTATTGTTTTACCAATTCATTTGGACCCTTGA
Protein sequence

>MsG0180001806.01.T01

MNFVNKQHLIINHVSEAHPPIQNHPDSGTNFPIVIIVVGMMATSFLLMAYYTFVIKCCFNWNNIDLVRGRRFSFSRQHEEQSTYSMTSDHRGLEQSVINSIPVIHYKEEKDYGELGISSECAFCLSEFQEDEILRVIPNCNHLFHIDCVDVWLQNNANCPLCRRKVSMTREIQVEHVVTPRPSPYLQSQNVENIIGGCEDFVVIDLDNNENDHEGQNLHEGRDERGKELEVPRDSHKKSIKLQKVSSMGDECIKDKDDGFLVQPIRRSFSMDLAIHKSLYQPSLHVHGVSSIEVCGDSSNRPKRSFFSFGHGSRSRSIVLPIHLDP*