AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006553.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006553.01.T01 MTR_2g010600 85.802 162 20 2 1 159 1 162 1.20e-98 281
MsG0280006553.01.T01 MTR_2g010590 78.616 159 34 0 1 159 1 159 2.29e-96 275
MsG0280006553.01.T01 MTR_4g050762 84.568 162 22 2 1 159 1 162 3.88e-90 260
MsG0280006553.01.T01 MTR_2g012370 84.568 162 22 2 1 159 1 162 3.88e-90 260
MsG0280006553.01.T01 MTR_2g010610 81.250 160 7 3 1 159 1 138 1.47e-78 229
MsG0280006553.01.T01 MTR_2g010650 68.263 167 43 6 1 159 1 165 1.15e-76 226
MsG0280006553.01.T01 MTR_2g010630 70.588 153 38 3 13 159 14 165 2.17e-75 222
MsG0280006553.01.T01 MTR_2g010700 60.355 169 56 5 1 159 1 168 4.77e-65 196
MsG0280006553.01.T01 MTR_2g010640 61.212 165 56 5 1 159 1 163 1.50e-63 192
MsG0280006553.01.T01 MTR_2g435490 54.487 156 69 2 6 159 9 164 1.42e-60 186
MsG0280006553.01.T01 MTR_2g010670 59.036 166 59 5 1 159 1 164 1.29e-58 180
MsG0280006553.01.T01 MTR_2g010690 56.627 166 63 5 1 159 1 164 4.27e-57 176
MsG0280006553.01.T01 MTR_2g010580 47.170 159 83 1 1 159 7 164 4.42e-54 168
MsG0280006553.01.T01 MTR_4g092540 49.375 160 70 3 1 159 10 159 2.32e-52 164
MsG0280006553.01.T01 MTR_4g128750 51.128 133 63 2 29 159 41 173 1.53e-42 139
MsG0280006553.01.T01 MTR_4g128770 44.526 137 71 2 28 159 70 206 3.36e-40 134
MsG0280006553.01.T01 MTR_1g067290 42.963 135 73 3 28 158 73 207 3.89e-34 119
MsG0280006553.01.T01 MTR_8g078770 35.329 167 94 5 7 159 6 172 7.84e-27 99.8
MsG0280006553.01.T01 MTR_4g092515 51.961 102 46 2 1 101 7 106 1.84e-26 97.4
MsG0280006553.01.T01 MTR_5g018770 34.270 178 95 6 3 159 9 185 2.25e-25 96.3
MsG0280006553.01.T01 MTR_5g018755 35.789 95 55 4 71 159 9 103 4.47e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006553.01.T01 AT2G14580 64.968 157 50 2 3 159 10 161 2.35e-70 209
MsG0280006553.01.T01 AT4G33720 61.146 157 58 2 3 159 10 163 3.12e-67 202
MsG0280006553.01.T01 AT2G14610 60.390 154 59 1 6 159 10 161 8.17e-64 193
MsG0280006553.01.T01 AT3G19690 55.828 163 66 3 1 159 1 161 2.27e-62 189
MsG0280006553.01.T01 AT5G26130 56.024 166 66 2 1 159 1 166 3.31e-61 186
MsG0280006553.01.T01 AT4G33710 60.000 140 54 1 22 159 27 166 5.02e-58 178
MsG0280006553.01.T01 AT1G50060 50.318 157 74 2 4 159 8 161 1.61e-50 159
MsG0280006553.01.T01 AT4G33730 54.135 133 59 2 28 159 41 172 2.65e-47 151
MsG0280006553.01.T01 AT2G19990 53.906 128 58 1 33 159 49 176 3.19e-47 151
MsG0280006553.01.T01 AT4G25790 51.471 136 64 2 26 159 75 210 1.55e-46 151
MsG0280006553.01.T01 AT5G57625 51.880 133 62 2 29 159 75 207 1.27e-45 148
MsG0280006553.01.T01 AT1G50050 50.000 158 74 3 4 159 8 162 6.91e-45 145
MsG0280006553.01.T01 AT3G09590 47.407 135 68 2 28 159 52 186 1.99e-43 142
MsG0280006553.01.T01 AT4G30320 44.966 149 79 3 13 159 14 161 2.37e-43 141
MsG0280006553.01.T01 AT4G25780 48.905 137 65 3 28 159 54 190 1.18e-42 140
MsG0280006553.01.T01 AT1G50050 48.718 156 75 3 4 157 8 160 7.65e-42 139
MsG0280006553.01.T01 AT4G07820 46.763 139 72 2 21 159 24 160 5.63e-41 135
MsG0280006553.01.T01 AT1G01310 47.015 134 68 2 28 159 87 219 2.14e-40 136
MsG0280006553.01.T01 AT5G02730 44.853 136 72 2 27 159 58 193 9.38e-40 133
MsG0280006553.01.T01 AT4G31470 43.704 135 75 1 26 159 51 185 7.64e-38 128
MsG0280006553.01.T01 AT5G66590 42.336 137 73 4 29 159 49 185 3.34e-31 111
MsG0280006553.01.T01 AT2G19970 43.262 141 67 7 30 159 39 177 1.09e-27 102
MsG0280006553.01.T01 AT2G19980 36.905 168 84 9 4 159 8 165 5.10e-23 89.7

Find 40 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCACTCCGGTGGCCGTTAC+GGG 0.273758 2:+3386273 MsG0280006553.01.T01:CDS
TTGGGCTTATTACTCATTAT+TGG 0.280398 2:+3386095 MsG0280006553.01.T01:CDS
ATCCACTCCGGTGGCCGTTA+CGG 0.326170 2:+3386272 MsG0280006553.01.T01:CDS
GCCATTATACTCAGGTTGTT+TGG 0.328715 2:+3386414 MsG0280006553.01.T01:CDS
CATAGTCTGCTTGTGAGTCT+TGG 0.335837 2:-3386133 None:intergenic
TTGGTGTTCAAAATATTGTT+TGG 0.341386 2:+3386186 MsG0280006553.01.T01:CDS
AATAGTAACACATGTGCTTC+TGG 0.347660 2:+3386380 MsG0280006553.01.T01:CDS
TATTAAGCAAGCACTTAGTA+TGG 0.369831 2:-3386539 None:intergenic
ATCTGAGCCACTCAAGTCTC+CGG 0.382702 2:-3386315 None:intergenic
TCCAAACAACCTGAGTATAA+TGG 0.390459 2:-3386415 None:intergenic
TGGTGTTCAAAATATTGTTT+GGG 0.400203 2:+3386187 MsG0280006553.01.T01:CDS
ATAGTCTGCTTGTGAGTCTT+GGG 0.401577 2:-3386132 None:intergenic
TCTGAGCCACTCAAGTCTCC+GGG 0.459067 2:-3386314 None:intergenic
CGTTATTATAGTTGCCTGGT+GGG 0.465282 2:-3386511 None:intergenic
GCCAAAGTGAGATGTGATAA+TGG 0.484296 2:+3386461 MsG0280006553.01.T01:CDS
TCCATTATCACATCTCACTT+TGG 0.498337 2:-3386462 None:intergenic
CCCCGTAACGGCCACCGGAG+TGG 0.509997 2:-3386274 None:intergenic
GAGAATCTCGCGGGTAGCCC+CGG 0.516219 2:+3386296 MsG0280006553.01.T01:CDS
TGGAGAAACTCACAACGTCT+TGG 0.520363 2:+3386434 MsG0280006553.01.T01:CDS
ATTCTCCCCGTAACGGCCAC+CGG 0.528380 2:-3386279 None:intergenic
GCTCAGATGCAGTGAAGTTG+TGG 0.543630 2:+3386330 MsG0280006553.01.T01:CDS
CTCAGATGCAGTGAAGTTGT+GGG 0.551141 2:+3386331 MsG0280006553.01.T01:CDS
TCGTTATTATAGTTGCCTGG+TGG 0.573306 2:-3386512 None:intergenic
ACTTGCAATTACGACCCACC+AGG 0.586857 2:+3386497 MsG0280006553.01.T01:CDS
GGCCACCGGAGTGGATCAGT+TGG 0.592030 2:-3386265 None:intergenic
CCACTCCGGTGGCCGTTACG+GGG 0.597515 2:+3386274 MsG0280006553.01.T01:CDS
CCGCGAGATTCTCCCCGTAA+CGG 0.609234 2:-3386286 None:intergenic
CCGTTACGGGGAGAATCTCG+CGG 0.614640 2:+3386286 MsG0280006553.01.T01:CDS
TGCCAACTGATCCACTCCGG+TGG 0.630736 2:+3386263 MsG0280006553.01.T01:CDS
CGTTACGGGGAGAATCTCGC+GGG 0.631764 2:+3386287 MsG0280006553.01.T01:CDS
AACGAAGCAAGATCTGAGGT+TGG 0.637008 2:+3386167 MsG0280006553.01.T01:CDS
AGACTATGCTAATCAACACA+AGG 0.643418 2:+3386235 MsG0280006553.01.T01:CDS
AGTGTGTGGCCATTATACTC+AGG 0.651003 2:+3386406 MsG0280006553.01.T01:CDS
GGTAGCCCCGGAGACTTGAG+TGG 0.652738 2:+3386308 MsG0280006553.01.T01:CDS
GATTGCCAACTGATCCACTC+CGG 0.665515 2:+3386260 MsG0280006553.01.T01:CDS
GACTATGCTAATCAACACAA+GGG 0.683970 2:+3386236 MsG0280006553.01.T01:CDS
TGTGCTTCTGGTAAAGTGTG+TGG 0.690422 2:+3386392 MsG0280006553.01.T01:CDS
TCACAACGAAGCAAGATCTG+AGG 0.699112 2:+3386163 MsG0280006553.01.T01:CDS
CTGAGCCACTCAAGTCTCCG+GGG 0.708120 2:-3386313 None:intergenic
AAAGTGAGATGTGATAATGG+AGG 0.800107 2:+3386464 MsG0280006553.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGGTGTTCAAAATATTGTTT+GGG + Chr2:3386187-3386206 MsG0280006553.01.T01:CDS 25.0%
!! ATTTTCTCTATTGTGTTTCT+TGG + Chr2:3386076-3386095 MsG0280006553.01.T01:CDS 25.0%
!! TTGGTGTTCAAAATATTGTT+TGG + Chr2:3386186-3386205 MsG0280006553.01.T01:CDS 25.0%
!! TTTTCTCTATTGTGTTTCTT+GGG + Chr2:3386077-3386096 MsG0280006553.01.T01:CDS 25.0%
TTGGGCTTATTACTCATTAT+TGG + Chr2:3386095-3386114 MsG0280006553.01.T01:CDS 30.0%
! TTTTCGTTATTATAGTTGCC+TGG - Chr2:3386518-3386537 None:intergenic 30.0%
AATAGTAACACATGTGCTTC+TGG + Chr2:3386380-3386399 MsG0280006553.01.T01:CDS 35.0%
AGACTATGCTAATCAACACA+AGG + Chr2:3386235-3386254 MsG0280006553.01.T01:CDS 35.0%
GACTATGCTAATCAACACAA+GGG + Chr2:3386236-3386255 MsG0280006553.01.T01:CDS 35.0%
TCCAAACAACCTGAGTATAA+TGG - Chr2:3386418-3386437 None:intergenic 35.0%
TCCATTATCACATCTCACTT+TGG - Chr2:3386465-3386484 None:intergenic 35.0%
!! AAAGTGAGATGTGATAATGG+AGG + Chr2:3386464-3386483 MsG0280006553.01.T01:CDS 35.0%
ATAGTCTGCTTGTGAGTCTT+GGG - Chr2:3386135-3386154 None:intergenic 40.0%
CGTTATTATAGTTGCCTGGT+GGG - Chr2:3386514-3386533 None:intergenic 40.0%
GCCAAAGTGAGATGTGATAA+TGG + Chr2:3386461-3386480 MsG0280006553.01.T01:CDS 40.0%
TCGTTATTATAGTTGCCTGG+TGG - Chr2:3386515-3386534 None:intergenic 40.0%
! GCCATTATACTCAGGTTGTT+TGG + Chr2:3386414-3386433 MsG0280006553.01.T01:CDS 40.0%
AGTGTGTGGCCATTATACTC+AGG + Chr2:3386406-3386425 MsG0280006553.01.T01:CDS 45.0%
CATAGTCTGCTTGTGAGTCT+TGG - Chr2:3386136-3386155 None:intergenic 45.0%
CTCAGATGCAGTGAAGTTGT+GGG + Chr2:3386331-3386350 MsG0280006553.01.T01:CDS 45.0%
TCACAACGAAGCAAGATCTG+AGG + Chr2:3386163-3386182 MsG0280006553.01.T01:CDS 45.0%
TGGAGAAACTCACAACGTCT+TGG + Chr2:3386434-3386453 MsG0280006553.01.T01:CDS 45.0%
TGTGCTTCTGGTAAAGTGTG+TGG + Chr2:3386392-3386411 MsG0280006553.01.T01:CDS 45.0%
! AACGAAGCAAGATCTGAGGT+TGG + Chr2:3386167-3386186 MsG0280006553.01.T01:CDS 45.0%
ACTTGCAATTACGACCCACC+AGG + Chr2:3386497-3386516 MsG0280006553.01.T01:CDS 50.0%
ATCTGAGCCACTCAAGTCTC+CGG - Chr2:3386318-3386337 None:intergenic 50.0%
GATTGCCAACTGATCCACTC+CGG + Chr2:3386260-3386279 MsG0280006553.01.T01:CDS 50.0%
GCTCAGATGCAGTGAAGTTG+TGG + Chr2:3386330-3386349 MsG0280006553.01.T01:CDS 50.0%
TCTGAGCCACTCAAGTCTCC+GGG - Chr2:3386317-3386336 None:intergenic 55.0%
ATCCACTCCGGTGGCCGTTA+CGG + Chr2:3386272-3386291 MsG0280006553.01.T01:CDS 60.0%
ATTCTCCCCGTAACGGCCAC+CGG - Chr2:3386282-3386301 None:intergenic 60.0%
CCGCGAGATTCTCCCCGTAA+CGG - Chr2:3386289-3386308 None:intergenic 60.0%
CCGTTACGGGGAGAATCTCG+CGG + Chr2:3386286-3386305 MsG0280006553.01.T01:CDS 60.0%
CGTTACGGGGAGAATCTCGC+GGG + Chr2:3386287-3386306 MsG0280006553.01.T01:CDS 60.0%
CTGAGCCACTCAAGTCTCCG+GGG - Chr2:3386316-3386335 None:intergenic 60.0%
TGCCAACTGATCCACTCCGG+TGG + Chr2:3386263-3386282 MsG0280006553.01.T01:CDS 60.0%
GAGAATCTCGCGGGTAGCCC+CGG + Chr2:3386296-3386315 MsG0280006553.01.T01:CDS 65.0%
GGCCACCGGAGTGGATCAGT+TGG - Chr2:3386268-3386287 None:intergenic 65.0%
GGTAGCCCCGGAGACTTGAG+TGG + Chr2:3386308-3386327 MsG0280006553.01.T01:CDS 65.0%
TCCACTCCGGTGGCCGTTAC+GGG + Chr2:3386273-3386292 MsG0280006553.01.T01:CDS 65.0%
CCACTCCGGTGGCCGTTACG+GGG + Chr2:3386274-3386293 MsG0280006553.01.T01:CDS 70.0%
CCCCGTAACGGCCACCGGAG+TGG - Chr2:3386277-3386296 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr2 gene 3386068 3386547 3386068 ID=MsG0280006553.01;Name=MsG0280006553.01
Chr2 mRNA 3386068 3386547 3386068 ID=MsG0280006553.01.T01;Parent=MsG0280006553.01;Name=MsG0280006553.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|159
Chr2 exon 3386068 3386547 3386068 ID=MsG0280006553.01.T01:exon:1661;Parent=MsG0280006553.01.T01
Chr2 CDS 3386068 3386547 3386068 ID=MsG0280006553.01.T01:cds;Parent=MsG0280006553.01.T01
Gene Sequence

>MsG0280006553.01.T01

ATGAGTTTATTTTCTCTATTGTGTTTCTTGGGCTTATTACTCATTATTGGTCATGTTGCACATGCCCAAGACTCACAAGCAGACTATGTGAACGCTCACAACGAAGCAAGATCTGAGGTTGGTGTTCAAAATATTGTTTGGGACAATACTGTTGCTAGTTTTGCACAAGACTATGCTAATCAACACAAGGGTGATTGCCAACTGATCCACTCCGGTGGCCGTTACGGGGAGAATCTCGCGGGTAGCCCCGGAGACTTGAGTGGCTCAGATGCAGTGAAGTTGTGGGTGAATGAGAAAGTTGACTATGATTATAATAGTAACACATGTGCTTCTGGTAAAGTGTGTGGCCATTATACTCAGGTTGTTTGGAGAAACTCACAACGTCTTGGATGTGCCAAAGTGAGATGTGATAATGGAGGCACATTCATTACTTGCAATTACGACCCACCAGGCAACTATAATAACGAAAAGCCATACTAA

Protein sequence

>MsG0280006553.01.T01

MSLFSLLCFLGLLLIIGHVAHAQDSQADYVNAHNEARSEVGVQNIVWDNTVASFAQDYANQHKGDCQLIHSGGRYGENLAGSPGDLSGSDAVKLWVNEKVDYDYNSNTCASGKVCGHYTQVVWRNSQRLGCAKVRCDNGGTFITCNYDPPGNYNNEKPY*