AlfalfaGEDB | MsG0180001867.01

M. sativa cultivar ZhongmuNo.1 / MsG0180001867.01

KEGG: K03320
GO: GO:0008519 GO:0015696 GO:0016020
PFAM: PF00909

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001867.01.T01	XP_003590177.1	98.462	65	1	0	1	65	428	492	1.39E-37	141
MsG0180001867.01.T02	XP_003590177.1	100	55	0	0	1	55	438	492	7.43E-31	122

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001867.01.T01	P58905	68.254	63	18	1	1	63	423	483	6.97E-24	96.3
MsG0180001867.01.T02	P54144	62.264	53	20	0	1	53	433	485	3.90E-18	79.3

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001867.01.T01	G7I6P4	98.462	65	1	0	1	65	428	492	6.66e-38	141
MsG0180001867.01.T02	G7I6P4	100.000	55	0	0	1	55	438	492	3.55e-31	122

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000585.01	MsG0180001867.01	0.820602	6.290535e-53	4.999543e-50
MsG0180001867.01	MsG0180001868.01	0.833210	6.078075e-56	6.952314e-53
MsG0180001867.01	MsG0180004005.01	0.830784	2.415783e-55	2.571321e-52
MsG0180001867.01	MsG0880042744.01	0.803566	3.279667e-49	1.653641e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001867.01.T01	MTR_1g045550	98.462	65	1	0	1	65	428	492	1.69e-41	141
MsG0180001867.01.T01	MTR_1g079760	76.000	50	12	0	1	50	430	479	1.74e-21	86.7
MsG0180001867.01.T01	MTR_7g098930	57.407	54	23	0	1	54	428	481	4.35e-16	71.2
MsG0180001867.01.T01	MTR_3g463730	53.125	64	29	1	1	63	394	457	2.05e-15	69.3
MsG0180001867.01.T01	MTR_7g113340	56.863	51	22	0	1	51	428	478	2.81e-15	68.9
MsG0180001867.01.T02	MTR_1g045550	100.000	55	0	0	1	55	438	492	8.99e-35	122
MsG0180001867.01.T02	MTR_1g079760	80.000	40	8	0	1	40	440	479	1.11e-16	72.4
MsG0180001867.01.T02	MTR_3g463730	53.704	54	24	1	1	53	404	457	4.40e-13	62.4
MsG0180001867.01.T02	MTR_7g098930	59.091	44	18	0	1	44	438	481	2.19e-12	60.5
MsG0180001867.01.T02	MTR_7g113340	58.537	41	17	0	1	41	438	478	3.59e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001867.01.T01	AT4G13510	65.079	63	22	0	1	63	423	485	1.40e-24	95.5
MsG0180001867.01.T01	AT1G64780	74.000	50	13	0	1	50	435	484	1.72e-22	89.4
MsG0180001867.01.T01	AT3G24300	66.071	56	17	1	1	54	427	482	3.55e-20	82.8
MsG0180001867.01.T01	AT4G28700	63.492	63	20	2	1	62	434	494	1.16e-19	81.6
MsG0180001867.01.T01	AT3G24290	69.388	49	15	0	1	49	426	474	3.89e-18	77.0
MsG0180001867.01.T02	AT4G13510	62.264	53	20	0	1	53	433	485	3.98e-19	79.3
MsG0180001867.01.T02	AT1G64780	77.500	40	9	0	1	40	445	484	2.40e-17	74.3
MsG0180001867.01.T02	AT4G28700	62.264	53	17	2	1	52	444	494	1.66e-14	66.2
MsG0180001867.01.T02	AT3G24300	63.043	46	15	1	1	44	437	482	4.40e-14	65.1
MsG0180001867.01.T02	AT3G24290	66.667	39	13	0	1	39	436	474	3.85e-12	59.7

Find 23 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATACAAATATGGTTATTAT+TGG	0.292735	1:-28412607	None:intergenic
GATGAAGGATCACATAGAAA+TGG	0.373243	1:-28412454	MsG0180001867.01.T01:CDS
TGGGTGGGTAAGTGCAACTA+TGG	0.381238	1:-28412587	MsG0180001867.01.T01:CDS
TGAAGATGAACTTGCGGGTA+TGG	0.421273	1:-28412515	MsG0180001867.01.T01:CDS
TCAACTGAAGATGAACTTGC+GGG	0.428559	1:-28412520	MsG0180001867.01.T01:CDS
ATACAAATATGGTTATTATT+GGG	0.465000	1:-28412606	None:intergenic
TTATTCAAAACAAAGAATAG+TGG	0.471377	1:+28412562	None:intergenic
ATGAAGGATCACATAGAAAT+GGG	0.473794	1:-28412453	MsG0180001867.01.T01:CDS
AACAATCACAAAATCGTAGT+TGG	0.478148	1:+28412386	None:intergenic
ATAAGCAAACCCACCATGTC+GGG	0.481927	1:+28412486	None:intergenic
GCTTATGCTTATGAAGATGA+AGG	0.489963	1:-28412469	MsG0180001867.01.T01:CDS
TGAATAAATTGAAGTTGTTG+AGG	0.497576	1:-28412546	MsG0180001867.01.T01:CDS
CAAATATGGTTATTATTGGG+TGG	0.504516	1:-28412603	None:intergenic
AAATATGGTTATTATTGGGT+GGG	0.521820	1:-28412602	None:intergenic
ATAGAAATGGGACTCAATTG+AGG	0.554439	1:-28412441	MsG0180001867.01.T01:CDS
CACAAAATCGTAGTTGGAGT+AGG	0.564558	1:+28412392	None:intergenic
TTCAACTGAAGATGAACTTG+CGG	0.585243	1:-28412521	MsG0180001867.01.T01:CDS
AATCGTAGTTGGAGTAGGAG+TGG	0.589761	1:+28412397	None:intergenic
GGGTGGGTAAGTGCAACTAT+GGG	0.598468	1:-28412586	MsG0180001867.01.T01:CDS
TGGATTTGACCCGACATGGT+GGG	0.598703	1:-28412495	MsG0180001867.01.T01:CDS
CATAAGCAAACCCACCATGT+CGG	0.601038	1:+28412485	None:intergenic
GGTATGGATTTGACCCGACA+TGG	0.625832	1:-28412499	MsG0180001867.01.T01:CDS
ATGGATTTGACCCGACATGG+TGG	0.636723	1:-28412496	MsG0180001867.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTATTCAAAACAAAGAATAG+TGG	+	Chr1:28412423-28412442	None:intergenic	20.0%
!	TGAATAAATTGAAGTTGTTG+AGG	-	Chr1:28412436-28412455	MsG0180001867.01.T01:CDS	25.0%
	AACAATCACAAAATCGTAGT+TGG	+	Chr1:28412599-28412618	None:intergenic	30.0%
	ATGAAGGATCACATAGAAAT+GGG	-	Chr1:28412529-28412548	MsG0180001867.01.T01:CDS	30.0%
	ATAGAAATGGGACTCAATTG+AGG	-	Chr1:28412541-28412560	MsG0180001867.01.T01:CDS	35.0%
	GATGAAGGATCACATAGAAA+TGG	-	Chr1:28412528-28412547	MsG0180001867.01.T01:CDS	35.0%
	GCTTATGCTTATGAAGATGA+AGG	-	Chr1:28412513-28412532	MsG0180001867.01.T01:CDS	35.0%
	TTCAACTGAAGATGAACTTG+CGG	-	Chr1:28412461-28412480	MsG0180001867.01.T01:CDS	35.0%
	CACAAAATCGTAGTTGGAGT+AGG	+	Chr1:28412593-28412612	None:intergenic	40.0%
	TCAACTGAAGATGAACTTGC+GGG	-	Chr1:28412462-28412481	MsG0180001867.01.T01:CDS	40.0%
	AATCGTAGTTGGAGTAGGAG+TGG	+	Chr1:28412588-28412607	None:intergenic	45.0%
	ATAAGCAAACCCACCATGTC+GGG	+	Chr1:28412499-28412518	None:intergenic	45.0%
	CATAAGCAAACCCACCATGT+CGG	+	Chr1:28412500-28412519	None:intergenic	45.0%
	TGAAGATGAACTTGCGGGTA+TGG	-	Chr1:28412467-28412486	MsG0180001867.01.T01:CDS	45.0%
	ATGGATTTGACCCGACATGG+TGG	-	Chr1:28412486-28412505	MsG0180001867.01.T01:CDS	50.0%
	GGGTGGGTAAGTGCAACTAT+GGG	-	Chr1:28412396-28412415	MsG0180001867.01.T01:CDS	50.0%
	GGTATGGATTTGACCCGACA+TGG	-	Chr1:28412483-28412502	MsG0180001867.01.T01:CDS	50.0%
	TGGATTTGACCCGACATGGT+GGG	-	Chr1:28412487-28412506	MsG0180001867.01.T01:CDS	50.0%
	TGGGTGGGTAAGTGCAACTA+TGG	-	Chr1:28412395-28412414	MsG0180001867.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	28412384	28412620	28412384	ID=MsG0180001867.01;Name=MsG0180001867.01
Chr1	mRNA	28412384	28412620	28412384	ID=MsG0180001867.01.T01;Parent=MsG0180001867.01;Name=MsG0180001867.01.T01;_AED=0.48;_eAED=0.48;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|78
Chr1	exon	28412384	28412620	28412384	ID=MsG0180001867.01.T01:exon:18848;Parent=MsG0180001867.01.T01
Chr1	CDS	28412384	28412620	28412384	ID=MsG0180001867.01.T01:cds;Parent=MsG0180001867.01.T01
Chr1	mRNA	28412384	28412590	28412384	ID=MsG0180001867.01.T02;Parent=MsG0180001867.01;Name=MsG0180001867.01.T02;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|68
Chr1	exon	28412384	28412590	28412384	ID=MsG0180001867.01.T02:exon:18849;Parent=MsG0180001867.01.T02
Chr1	CDS	28412384	28412590	28412384	ID=MsG0180001867.01.T02:cds;Parent=MsG0180001867.01.T02

Gene Sequence

>MsG0180001867.01.T01

ATGGTTATTATTGGGTGGGTAAGTGCAACTATGGGTCCACTATTCTTTGTTTTGAATAAATTGAAGTTGTTGAGGATTTCAACTGAAGATGAACTTGCGGGTATGGATTTGACCCGACATGGTGGGTTTGCTTATGCTTATGAAGATGAAGGATCACATAGAAATGGGACTCAATTGAGGAAAATTGATCATAATCTTCTTCCACTCCTACTCCAACTACGATTTTGTGATTGTTAA

>MsG0180001867.01.T02

ATGGGTCCACTATTCTTTGTTTTGAATAAATTGAAGTTGTTGAGGATTTCAACTGAAGATGAACTTGCGGGTATGGATTTGACCCGACATGGTGGGTTTGCTTATGCTTATGAAGATGAAGGATCACATAGAAATGGGACTCAATTGAGGAAAATTGATCATAATCTTCTTCCACTCCTACTCCAACTACGATTTTGTGATTGTTAA

Protein sequence

>MsG0180001867.01.T01

MVIIGWVSATMGPLFFVLNKLKLLRISTEDELAGMDLTRHGGFAYAYEDEGSHRNGTQLRKIDHNLLPLLLQLRFCDC*

>MsG0180001867.01.T02

MGPLFFVLNKLKLLRISTEDELAGMDLTRHGGFAYAYEDEGSHRNGTQLRKIDHNLLPLLLQLRFCDC*