Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002303.01.T01 | KEH39709.1 | 87.713 | 293 | 9 | 1 | 4 | 269 | 155 | 447 | 0 | 520 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002303.01.T01 | Q9FN03 | 31.78 | 236 | 123 | 8 | 21 | 240 | 161 | 374 | 5.71E-20 | 92 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002303.01.T01 | A0A072VCE3 | 87.713 | 293 | 9 | 1 | 4 | 269 | 155 | 447 | 0.0 | 520 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002298.01 | MsG0180002303.01 | 0.873473 | 1.431086e-67 | 6.219183e-64 |
| MsG0180002303.01 | MsG0180002315.01 | 0.822981 | 1.770999e-53 | 1.505392e-50 |
| MsG0180002303.01 | MsG0480021360.01 | 0.800058 | 1.722302e-48 | 7.942494e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002303.01.T01 | MTR_1g006695 | 87.713 | 293 | 9 | 1 | 4 | 269 | 155 | 447 | 0.0 | 520 |
| MsG0180002303.01.T01 | MTR_3g109650 | 59.386 | 293 | 87 | 5 | 4 | 268 | 71 | 359 | 3.70e-111 | 329 |
| MsG0180002303.01.T01 | MTR_3g109650 | 59.386 | 293 | 87 | 5 | 4 | 268 | 169 | 457 | 1.19e-109 | 328 |
| MsG0180002303.01.T01 | MTR_8g094410 | 31.174 | 247 | 125 | 9 | 13 | 240 | 17 | 237 | 1.09e-22 | 95.5 |
| MsG0180002303.01.T01 | MTR_8g094410 | 30.739 | 257 | 130 | 10 | 6 | 240 | 92 | 322 | 3.59e-22 | 95.1 |
| MsG0180002303.01.T01 | MTR_8g094410 | 29.130 | 230 | 114 | 9 | 34 | 240 | 15 | 218 | 3.21e-18 | 84.0 |
| MsG0180002303.01.T01 | MTR_8g094410 | 30.739 | 257 | 130 | 10 | 6 | 240 | 92 | 322 | 5.76e-22 | 94.0 |
| MsG0180002303.01.T01 | MTR_8g094410 | 29.130 | 230 | 114 | 9 | 34 | 240 | 15 | 218 | 4.40e-18 | 83.2 |
| MsG0180002303.01.T01 | MTR_8g094410 | 31.174 | 247 | 125 | 9 | 13 | 240 | 151 | 371 | 6.00e-22 | 94.7 |
| MsG0180002303.01.T01 | MTR_8g094410 | 27.731 | 238 | 132 | 8 | 20 | 240 | 53 | 267 | 3.98e-17 | 80.9 |
| MsG0180002303.01.T01 | MTR_8g094410 | 30.000 | 230 | 110 | 9 | 34 | 240 | 15 | 216 | 4.86e-17 | 80.9 |
| MsG0180002303.01.T01 | MTR_8g094410 | 34.483 | 145 | 80 | 6 | 99 | 241 | 34 | 165 | 1.68e-11 | 64.3 |
| MsG0180002303.01.T01 | MTR_8g094410 | 31.933 | 238 | 117 | 9 | 22 | 240 | 160 | 371 | 1.15e-21 | 93.6 |
| MsG0180002303.01.T01 | MTR_8g094410 | 27.731 | 238 | 132 | 8 | 20 | 240 | 53 | 267 | 7.02e-17 | 80.1 |
| MsG0180002303.01.T01 | MTR_8g094410 | 30.000 | 230 | 110 | 9 | 34 | 240 | 15 | 216 | 8.50e-17 | 79.7 |
| MsG0180002303.01.T01 | MTR_8g094410 | 35.374 | 147 | 76 | 7 | 99 | 241 | 34 | 165 | 2.97e-11 | 63.5 |
| MsG0180002303.01.T01 | MTR_8g094410 | 32.735 | 223 | 105 | 9 | 37 | 240 | 2 | 198 | 6.42e-21 | 89.7 |
| MsG0180002303.01.T01 | MTR_3g096780 | 29.740 | 269 | 150 | 8 | 13 | 266 | 153 | 397 | 1.31e-20 | 90.9 |
| MsG0180002303.01.T01 | MTR_3g096780 | 29.258 | 229 | 117 | 7 | 32 | 240 | 15 | 218 | 4.01e-17 | 80.9 |
| MsG0180002303.01.T01 | MTR_3g096780 | 28.085 | 235 | 129 | 8 | 20 | 237 | 55 | 266 | 8.02e-16 | 77.0 |
| MsG0180002303.01.T01 | MTR_0095s0070 | 28.151 | 238 | 131 | 9 | 20 | 240 | 49 | 263 | 1.25e-17 | 80.9 |
| MsG0180002303.01.T01 | MTR_0095s0070 | 30.000 | 220 | 112 | 8 | 44 | 240 | 12 | 212 | 4.16e-16 | 76.6 |
| MsG0180002303.01.T01 | MTR_0095s0070 | 35.374 | 147 | 76 | 7 | 99 | 241 | 30 | 161 | 1.31e-11 | 63.9 |
| MsG0180002303.01.T01 | MTR_7g086050 | 28.708 | 209 | 116 | 8 | 21 | 212 | 233 | 425 | 2.80e-14 | 73.2 |
| MsG0180002303.01.T01 | MTR_3g069030 | 28.713 | 202 | 98 | 8 | 31 | 211 | 286 | 462 | 1.28e-12 | 67.8 |
| MsG0180002303.01.T01 | MTR_3g069030 | 27.016 | 248 | 121 | 8 | 31 | 240 | 214 | 439 | 6.08e-11 | 62.8 |
| MsG0180002303.01.T01 | MTR_1g042800 | 24.506 | 253 | 137 | 9 | 19 | 240 | 352 | 581 | 2.15e-12 | 67.4 |
| MsG0180002303.01.T01 | MTR_4g029410 | 27.969 | 261 | 136 | 11 | 19 | 252 | 159 | 394 | 2.41e-12 | 67.4 |
| MsG0180002303.01.T01 | MTR_0024s0070 | 33.784 | 148 | 81 | 6 | 97 | 240 | 213 | 347 | 4.77e-12 | 65.9 |
| MsG0180002303.01.T01 | MTR_7g016640 | 25.000 | 220 | 123 | 9 | 35 | 231 | 65 | 265 | 2.25e-11 | 63.9 |
| MsG0180002303.01.T01 | MTR_7g016640 | 24.528 | 212 | 134 | 5 | 35 | 231 | 65 | 265 | 2.65e-11 | 63.5 |
| MsG0180002303.01.T01 | MTR_3g097060 | 23.288 | 219 | 135 | 7 | 52 | 249 | 26 | 232 | 4.55e-11 | 62.8 |
| MsG0180002303.01.T01 | MTR_3g086300 | 29.536 | 237 | 119 | 10 | 21 | 233 | 241 | 453 | 4.61e-11 | 63.2 |
| MsG0180002303.01.T01 | MTR_3g097060 | 23.288 | 219 | 135 | 7 | 52 | 249 | 26 | 232 | 4.77e-11 | 62.8 |
| MsG0180002303.01.T01 | MTR_1g099220 | 24.904 | 261 | 145 | 9 | 19 | 252 | 372 | 608 | 5.14e-11 | 63.2 |
| MsG0180002303.01.T01 | MTR_3g064560 | 31.852 | 135 | 80 | 6 | 97 | 230 | 324 | 447 | 8.10e-11 | 62.8 |
| MsG0180002303.01.T01 | MTR_5g089900 | 24.809 | 262 | 144 | 9 | 19 | 252 | 418 | 654 | 9.70e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002303.01.T01 | AT5G11580 | 51.493 | 268 | 101 | 4 | 4 | 243 | 16 | 282 | 1.20e-86 | 264 |
| MsG0180002303.01.T01 | AT5G11580 | 51.493 | 268 | 101 | 4 | 4 | 243 | 167 | 433 | 4.25e-85 | 265 |
| MsG0180002303.01.T01 | AT5G63860 | 31.780 | 236 | 123 | 8 | 21 | 240 | 161 | 374 | 5.82e-21 | 92.0 |
| MsG0180002303.01.T01 | AT5G63860 | 30.901 | 233 | 122 | 8 | 22 | 237 | 57 | 267 | 6.06e-20 | 89.0 |
| MsG0180002303.01.T01 | AT5G63860 | 28.879 | 232 | 114 | 8 | 32 | 240 | 15 | 218 | 5.45e-17 | 80.5 |
| MsG0180002303.01.T01 | AT5G12350 | 28.326 | 233 | 122 | 6 | 34 | 240 | 310 | 523 | 1.02e-15 | 77.4 |
| MsG0180002303.01.T01 | AT5G12350 | 26.000 | 250 | 136 | 8 | 19 | 240 | 399 | 627 | 1.38e-12 | 68.2 |
| MsG0180002303.01.T01 | AT5G19420 | 27.468 | 233 | 124 | 6 | 34 | 240 | 314 | 527 | 1.92e-14 | 73.6 |
| MsG0180002303.01.T01 | AT5G19420 | 25.200 | 250 | 138 | 8 | 19 | 240 | 403 | 631 | 8.24e-11 | 62.8 |
| MsG0180002303.01.T01 | AT5G19420 | 27.468 | 233 | 124 | 6 | 34 | 240 | 348 | 561 | 1.96e-14 | 73.6 |
| MsG0180002303.01.T01 | AT5G19420 | 25.200 | 250 | 138 | 8 | 19 | 240 | 437 | 665 | 9.74e-11 | 62.4 |
| MsG0180002303.01.T01 | AT5G19420 | 27.468 | 233 | 124 | 6 | 34 | 240 | 314 | 527 | 2.05e-14 | 73.6 |
| MsG0180002303.01.T01 | AT5G19420 | 25.200 | 250 | 138 | 8 | 19 | 240 | 403 | 631 | 8.83e-11 | 62.8 |
| MsG0180002303.01.T01 | AT3G23270 | 37.589 | 141 | 78 | 5 | 101 | 240 | 273 | 404 | 5.28e-14 | 72.4 |
| MsG0180002303.01.T01 | AT3G23270 | 25.769 | 260 | 143 | 9 | 19 | 252 | 344 | 579 | 7.60e-12 | 65.9 |
| MsG0180002303.01.T01 | AT3G23270 | 37.589 | 141 | 78 | 5 | 101 | 240 | 277 | 408 | 5.38e-14 | 72.4 |
| MsG0180002303.01.T01 | AT3G23270 | 25.769 | 260 | 143 | 9 | 19 | 252 | 348 | 583 | 7.97e-12 | 65.9 |
| MsG0180002303.01.T01 | AT3G55580 | 29.000 | 200 | 98 | 8 | 31 | 210 | 310 | 485 | 1.29e-13 | 70.9 |
| MsG0180002303.01.T01 | AT3G26100 | 30.180 | 222 | 115 | 9 | 34 | 228 | 204 | 412 | 1.27e-12 | 67.8 |
| MsG0180002303.01.T01 | AT3G26100 | 30.180 | 222 | 115 | 9 | 34 | 228 | 204 | 412 | 1.27e-12 | 67.8 |
| MsG0180002303.01.T01 | AT3G26100 | 30.180 | 222 | 115 | 9 | 34 | 228 | 204 | 412 | 1.27e-12 | 67.8 |
| MsG0180002303.01.T01 | AT3G03790 | 25.962 | 208 | 123 | 7 | 21 | 212 | 233 | 425 | 1.49e-12 | 67.8 |
| MsG0180002303.01.T01 | AT3G26100 | 30.180 | 222 | 115 | 9 | 34 | 228 | 304 | 512 | 1.56e-12 | 67.4 |
| MsG0180002303.01.T01 | AT3G26100 | 30.180 | 222 | 115 | 9 | 34 | 228 | 304 | 512 | 1.56e-12 | 67.4 |
| MsG0180002303.01.T01 | AT3G26100 | 30.180 | 222 | 115 | 9 | 34 | 228 | 304 | 512 | 1.56e-12 | 67.4 |
| MsG0180002303.01.T01 | AT3G03790 | 26.794 | 209 | 120 | 8 | 21 | 212 | 230 | 422 | 1.57e-12 | 67.8 |
| MsG0180002303.01.T01 | AT3G03790 | 25.962 | 208 | 123 | 7 | 21 | 212 | 233 | 425 | 1.75e-12 | 67.8 |
| MsG0180002303.01.T01 | AT5G08710 | 25.391 | 256 | 150 | 7 | 23 | 251 | 179 | 420 | 2.01e-12 | 67.0 |
| MsG0180002303.01.T01 | AT3G15430 | 27.419 | 248 | 132 | 9 | 21 | 240 | 205 | 432 | 8.34e-12 | 65.5 |
| MsG0180002303.01.T01 | AT3G15430 | 27.419 | 248 | 132 | 9 | 21 | 240 | 205 | 432 | 8.34e-12 | 65.5 |
| MsG0180002303.01.T01 | AT3G15430 | 27.419 | 248 | 132 | 9 | 21 | 240 | 205 | 432 | 8.34e-12 | 65.5 |
| MsG0180002303.01.T01 | AT3G15430 | 27.419 | 248 | 132 | 9 | 21 | 240 | 205 | 432 | 8.34e-12 | 65.5 |
| MsG0180002303.01.T01 | AT3G15430 | 27.419 | 248 | 132 | 9 | 21 | 240 | 205 | 432 | 8.34e-12 | 65.5 |
| MsG0180002303.01.T01 | AT3G15430 | 27.419 | 248 | 132 | 9 | 21 | 240 | 205 | 432 | 8.34e-12 | 65.5 |
| MsG0180002303.01.T01 | AT1G27060 | 28.910 | 211 | 108 | 7 | 33 | 224 | 49 | 236 | 9.85e-12 | 65.1 |
| MsG0180002303.01.T01 | AT3G02300 | 27.311 | 238 | 133 | 7 | 33 | 242 | 178 | 403 | 1.36e-11 | 64.7 |
| MsG0180002303.01.T01 | AT3G02300 | 27.311 | 238 | 133 | 7 | 33 | 242 | 167 | 392 | 1.49e-11 | 64.7 |
| MsG0180002303.01.T01 | AT3G53830 | 28.364 | 275 | 132 | 9 | 12 | 240 | 192 | 447 | 1.86e-11 | 64.3 |
| MsG0180002303.01.T01 | AT5G16040 | 31.973 | 147 | 83 | 6 | 95 | 237 | 212 | 345 | 2.15e-11 | 63.9 |
| MsG0180002303.01.T01 | AT5G16040 | 28.358 | 201 | 104 | 6 | 45 | 213 | 198 | 390 | 2.91e-11 | 63.5 |
| MsG0180002303.01.T01 | AT3G53830 | 27.636 | 275 | 134 | 8 | 12 | 240 | 220 | 475 | 2.49e-11 | 63.9 |
| MsG0180002303.01.T01 | AT4G14368 | 25.403 | 248 | 138 | 8 | 19 | 240 | 363 | 589 | 4.43e-11 | 63.5 |
| MsG0180002303.01.T01 | AT4G14368 | 25.403 | 248 | 138 | 8 | 19 | 240 | 356 | 582 | 4.54e-11 | 63.5 |
| MsG0180002303.01.T01 | AT4G14368 | 25.403 | 248 | 138 | 8 | 19 | 240 | 361 | 587 | 5.46e-11 | 63.2 |
| MsG0180002303.01.T01 | AT1G69710 | 31.056 | 161 | 84 | 6 | 23 | 162 | 450 | 604 | 5.46e-11 | 63.2 |
| MsG0180002303.01.T01 | AT1G69710 | 24.896 | 241 | 132 | 6 | 28 | 240 | 403 | 622 | 5.93e-11 | 63.2 |
| MsG0180002303.01.T01 | AT4G14368 | 25.403 | 248 | 138 | 8 | 19 | 240 | 363 | 589 | 5.51e-11 | 63.2 |
| MsG0180002303.01.T01 | AT1G69710 | 31.056 | 161 | 84 | 6 | 23 | 162 | 437 | 591 | 6.26e-11 | 63.2 |
| MsG0180002303.01.T01 | AT1G69710 | 24.896 | 241 | 132 | 6 | 28 | 240 | 390 | 609 | 6.73e-11 | 62.8 |
| MsG0180002303.01.T01 | AT3G47660 | 31.690 | 142 | 85 | 6 | 101 | 240 | 176 | 307 | 6.92e-11 | 62.8 |
| MsG0180002303.01.T01 | AT3G47660 | 33.099 | 142 | 83 | 7 | 101 | 240 | 321 | 452 | 6.94e-11 | 62.8 |
| MsG0180002303.01.T01 | AT3G47660 | 29.801 | 151 | 76 | 6 | 101 | 240 | 318 | 449 | 7.07e-11 | 62.8 |
| MsG0180002303.01.T01 | AT3G47660 | 29.801 | 151 | 76 | 6 | 101 | 240 | 318 | 449 | 7.07e-11 | 62.8 |
| MsG0180002303.01.T01 | AT3G47660 | 29.801 | 151 | 76 | 6 | 101 | 240 | 321 | 452 | 7.61e-11 | 62.8 |
| MsG0180002303.01.T01 | AT3G02510 | 33.962 | 159 | 89 | 7 | 102 | 255 | 161 | 308 | 8.99e-11 | 62.0 |
| MsG0180002303.01.T01 | AT3G47660 | 29.801 | 151 | 76 | 6 | 101 | 240 | 357 | 488 | 9.39e-11 | 62.4 |
Find 76 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACATTTGGTCTTGGTGAAAA+TGG | 0.266531 | 1:-36479467 | MsG0180002303.01.T01:CDS |
| GGCAATTCTTGAACAAGTTC+AGG | 0.304428 | 1:+36479407 | None:intergenic |
| ACATTCCCACTTTAGTGTTA+TGG | 0.311232 | 1:-36479067 | MsG0180002303.01.T01:CDS |
| ATGATCTCATTTGTTACTCT+TGG | 0.325653 | 1:-36479675 | MsG0180002303.01.T01:CDS |
| TGATCTCATTTGTTACTCTT+GGG | 0.348009 | 1:-36479674 | MsG0180002303.01.T01:CDS |
| GGATATAAAGTTTGGTCTTT+CGG | 0.350572 | 1:-36479137 | MsG0180002303.01.T01:CDS |
| AACAACAATCATGGTCAATT+AGG | 0.358789 | 1:-36479649 | MsG0180002303.01.T01:CDS |
| ATTTCACTCCAACACCAGTA+TGG | 0.359909 | 1:-36479792 | MsG0180002303.01.T01:CDS |
| CCCAAGAGGGATATAAAGTT+TGG | 0.374797 | 1:-36479145 | MsG0180002303.01.T01:CDS |
| TGGGGAATGGAGAAGGGTCT+TGG | 0.396465 | 1:-36479305 | MsG0180002303.01.T01:CDS |
| CTTGTGTTGTTTCGTCGGAC+GGG | 0.396749 | 1:-36479340 | MsG0180002303.01.T01:CDS |
| AGAGGAAGAAGTGGTGTTCT+TGG | 0.401196 | 1:-36479113 | MsG0180002303.01.T01:CDS |
| AAAGAAGGTATATTCTCAAT+AGG | 0.401673 | 1:-36479820 | MsG0180002303.01.T01:CDS |
| ACCAAACTTTATATCCCTCT+TGG | 0.406767 | 1:+36479144 | None:intergenic |
| TATCCGGATGCGCGAGTTGT+TGG | 0.412533 | 1:-36479278 | MsG0180002303.01.T01:CDS |
| GAAAGTGTGTGTTGGACATT+TGG | 0.413664 | 1:-36479482 | MsG0180002303.01.T01:CDS |
| AATCATGGTCAATTAGGCTT+AGG | 0.416968 | 1:-36479643 | MsG0180002303.01.T01:CDS |
| TATTGTAAGCAATCCTTATG+AGG | 0.417836 | 1:-36479225 | MsG0180002303.01.T01:CDS |
| TCACCAACAACTCGCGCATC+CGG | 0.421376 | 1:+36479275 | None:intergenic |
| GGACGGGGATGTTTGGTCGT+GGG | 0.423753 | 1:-36479324 | MsG0180002303.01.T01:CDS |
| TTTCGTCGGACGGGGATGTT+TGG | 0.425452 | 1:-36479331 | MsG0180002303.01.T01:CDS |
| CAATCTTGGTGCCCTATGGA+TGG | 0.435071 | 1:-36479865 | None:intergenic |
| GGATGTTTGGTCGTGGGGAA+TGG | 0.440538 | 1:-36479318 | MsG0180002303.01.T01:CDS |
| AGAAGTGGTGTTCTTGGAAA+TGG | 0.441734 | 1:-36479107 | MsG0180002303.01.T01:CDS |
| ATCATGGTCAATTAGGCTTA+GGG | 0.441736 | 1:-36479642 | MsG0180002303.01.T01:CDS |
| CCAAACTTTATATCCCTCTT+GGG | 0.449742 | 1:+36479145 | None:intergenic |
| GAAACAAGATTTACAAATTG+TGG | 0.452095 | 1:+36479386 | None:intergenic |
| GCGCCACATGCAACTTGAAC+CGG | 0.459245 | 1:+36479188 | None:intergenic |
| GAAGGGTCTTGGTTTGTATC+CGG | 0.461196 | 1:-36479294 | MsG0180002303.01.T01:CDS |
| CGATAGACCAAATCCAGAAA+TGG | 0.467053 | 1:-36479611 | MsG0180002303.01.T01:CDS |
| GTGTGTTGGACATTTGGTCT+TGG | 0.480962 | 1:-36479476 | MsG0180002303.01.T01:CDS |
| TTGTGTTGTTTCGTCGGACG+GGG | 0.482207 | 1:-36479339 | MsG0180002303.01.T01:CDS |
| TTTAGTGTTATGGCCTCCAC+TGG | 0.484787 | 1:-36479057 | MsG0180002303.01.T01:CDS |
| AGTCTTCGCAAGCAAGATAA+GGG | 0.486128 | 1:-36478999 | MsG0180002303.01.T01:CDS |
| AAAGTTTGGTCTTTCGGAAG+AGG | 0.488876 | 1:-36479131 | MsG0180002303.01.T01:CDS |
| TCATACTTGTGTTGTTTCGT+CGG | 0.493278 | 1:-36479345 | MsG0180002303.01.T01:CDS |
| ACTTGTGTTGTTTCGTCGGA+CGG | 0.501899 | 1:-36479341 | MsG0180002303.01.T01:CDS |
| ATCTTGGTGCCCTATGGATG+GGG | 0.505271 | 1:-36479863 | None:intergenic |
| GAGTCTTCGCAAGCAAGATA+AGG | 0.506877 | 1:-36479000 | MsG0180002303.01.T01:CDS |
| TTGGTCGTGGGGAATGGAGA+AGG | 0.511138 | 1:-36479312 | MsG0180002303.01.T01:CDS |
| GATCTCATTTGTTACTCTTG+GGG | 0.511727 | 1:-36479673 | MsG0180002303.01.T01:CDS |
| ATTATAGCACTAGTTAGTGT+TGG | 0.512070 | 1:-36479721 | MsG0180002303.01.T01:CDS |
| ATACCTTCTTTGCTTTGATG+TGG | 0.514646 | 1:+36479832 | None:intergenic |
| CGGACGGGGATGTTTGGTCG+TGG | 0.518856 | 1:-36479325 | MsG0180002303.01.T01:CDS |
| AGTGTTATGGCCTCCACTGG+TGG | 0.519776 | 1:-36479054 | MsG0180002303.01.T01:CDS |
| TGGAAATCCCATACTGGTGT+TGG | 0.534551 | 1:+36479784 | None:intergenic |
| TTTCACTCCAACACCAGTAT+GGG | 0.536712 | 1:-36479791 | MsG0180002303.01.T01:CDS |
| TGTCCACATCAAAGCAAAGA+AGG | 0.542595 | 1:-36479835 | MsG0180002303.01.T01:CDS |
| AATCTTGGTGCCCTATGGAT+GGG | 0.548417 | 1:-36479864 | None:intergenic |
| ACATTTGATCAAGATTCTCA+TGG | 0.551383 | 1:-36479583 | MsG0180002303.01.T01:intron |
| TCTTGGGGTAACAACAATCA+TGG | 0.553129 | 1:-36479658 | MsG0180002303.01.T01:CDS |
| TCTTGCTTGCGAAGACTCGA+TGG | 0.556926 | 1:+36479005 | None:intergenic |
| TGGAGGCCATAACACTAAAG+TGG | 0.557548 | 1:+36479061 | None:intergenic |
| GACGGGGATGTTTGGTCGTG+GGG | 0.558285 | 1:-36479323 | MsG0180002303.01.T01:CDS |
| CTTGGTGAAAATGGACAACT+TGG | 0.566883 | 1:-36479458 | MsG0180002303.01.T01:CDS |
| ATACTGGTGTTGGAGTGAAA+TGG | 0.571831 | 1:+36479794 | None:intergenic |
| TGACCATGGAAATCCCATAC+TGG | 0.572116 | 1:+36479778 | None:intergenic |
| ACTATTATTGTTGCCCAAGA+GGG | 0.572627 | 1:-36479158 | MsG0180002303.01.T01:CDS |
| TGGTCGTGGGGAATGGAGAA+GGG | 0.577448 | 1:-36479311 | MsG0180002303.01.T01:CDS |
| TAAGGATTGCTTACAATAAG+CGG | 0.586907 | 1:+36479230 | None:intergenic |
| AATCTTGTTTCTGTTGACTG+TGG | 0.587618 | 1:-36479374 | MsG0180002303.01.T01:CDS |
| TACTATTATTGTTGCCCAAG+AGG | 0.588315 | 1:-36479159 | MsG0180002303.01.T01:CDS |
| TGAAAATCTTCCACCAGTGG+AGG | 0.601227 | 1:+36479044 | None:intergenic |
| TCAATTAGGCTTAGGGGACA+CGG | 0.606430 | 1:-36479635 | MsG0180002303.01.T01:CDS |
| GGAGGCCATAACACTAAAGT+GGG | 0.606579 | 1:+36479062 | None:intergenic |
| GAAAATGGACAACTTGGTCA+TGG | 0.608046 | 1:-36479452 | MsG0180002303.01.T01:CDS |
| AGATGAAAATCTTCCACCAG+TGG | 0.610842 | 1:+36479041 | None:intergenic |
| GAACCGGTTCAAGTTGCATG+TGG | 0.613809 | 1:-36479191 | MsG0180002303.01.T01:CDS |
| ATACATTAGAAAGTGTGTGT+TGG | 0.618605 | 1:-36479490 | MsG0180002303.01.T01:intron |
| TCTTTCGGAAGAGGAAGAAG+TGG | 0.632514 | 1:-36479122 | MsG0180002303.01.T01:CDS |
| GGTTCAAGTTGCATGTGGCG+CGG | 0.633426 | 1:-36479186 | MsG0180002303.01.T01:CDS |
| ACACCAGTATGGGATTTCCA+TGG | 0.640967 | 1:-36479781 | MsG0180002303.01.T01:CDS |
| TCATGGTCAATTAGGCTTAG+GGG | 0.643451 | 1:-36479641 | MsG0180002303.01.T01:CDS |
| ACAGTAGTAAAAGTTGCATG+TGG | 0.647702 | 1:-36479754 | MsG0180002303.01.T01:CDS |
| TCTTGGTGCCCTATGGATGG+GGG | 0.657298 | 1:-36479862 | None:intergenic |
| AGTGTTGGAGAATCATACAA+TGG | 0.686513 | 1:-36479706 | MsG0180002303.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCAATTTATGAAATATCATG+TGG | - | Chr1:36479273-36479292 | MsG0180002303.01.T01:CDS | 20.0% |
| ! | CTTTCTAATGTATCATCATT+AGG | + | Chr1:36479336-36479355 | None:intergenic | 25.0% |
| ! | CTTTGTTAACTCATAGAAAA+AGG | - | Chr1:36479310-36479329 | MsG0180002303.01.T01:CDS | 25.0% |
| ! | GAAACAAGATTTACAAATTG+TGG | + | Chr1:36479450-36479469 | None:intergenic | 25.0% |
| !! | AAAGAAGGTATATTCTCAAT+AGG | - | Chr1:36479013-36479032 | MsG0180002303.01.T01:CDS | 25.0% |
| !!! | TCAAATGTTTTTACCATTTC+TGG | + | Chr1:36479238-36479257 | None:intergenic | 25.0% |
| AACAACAATCATGGTCAATT+AGG | - | Chr1:36479184-36479203 | MsG0180002303.01.T01:CDS | 30.0% | |
| ACATTTGATCAAGATTCTCA+TGG | - | Chr1:36479250-36479269 | MsG0180002303.01.T01:CDS | 30.0% | |
| ATTATAGCACTAGTTAGTGT+TGG | - | Chr1:36479112-36479131 | MsG0180002303.01.T01:CDS | 30.0% | |
| TAAGGATTGCTTACAATAAG+CGG | + | Chr1:36479606-36479625 | None:intergenic | 30.0% | |
| TATTGTAAGCAATCCTTATG+AGG | - | Chr1:36479608-36479627 | MsG0180002303.01.T01:CDS | 30.0% | |
| TCTAAAAACTTAGCCTCATA+AGG | + | Chr1:36479624-36479643 | None:intergenic | 30.0% | |
| ! | AGATTTTCATCTCAAACAAG+AGG | - | Chr1:36479803-36479822 | MsG0180002303.01.T01:CDS | 30.0% |
| ! | ATACATTAGAAAGTGTGTGT+TGG | - | Chr1:36479343-36479362 | MsG0180002303.01.T01:CDS | 30.0% |
| ! | ATGATCTCATTTGTTACTCT+TGG | - | Chr1:36479158-36479177 | MsG0180002303.01.T01:CDS | 30.0% |
| ! | GGATATAAAGTTTGGTCTTT+CGG | - | Chr1:36479696-36479715 | MsG0180002303.01.T01:CDS | 30.0% |
| ! | TGATCTCATTTGTTACTCTT+GGG | - | Chr1:36479159-36479178 | MsG0180002303.01.T01:CDS | 30.0% |
| !! | GTTTTTACCATTTCTGGATT+TGG | + | Chr1:36479232-36479251 | None:intergenic | 30.0% |
| AATCATGGTCAATTAGGCTT+AGG | - | Chr1:36479190-36479209 | MsG0180002303.01.T01:CDS | 35.0% | |
| ACAGTAGTAAAAGTTGCATG+TGG | - | Chr1:36479079-36479098 | MsG0180002303.01.T01:CDS | 35.0% | |
| ACATTCCCACTTTAGTGTTA+TGG | - | Chr1:36479766-36479785 | MsG0180002303.01.T01:CDS | 35.0% | |
| ACCAAACTTTATATCCCTCT+TGG | + | Chr1:36479692-36479711 | None:intergenic | 35.0% | |
| ACTATTATTGTTGCCCAAGA+GGG | - | Chr1:36479675-36479694 | MsG0180002303.01.T01:CDS | 35.0% | |
| ATACCTTCTTTGCTTTGATG+TGG | + | Chr1:36479004-36479023 | None:intergenic | 35.0% | |
| ATCATGGTCAATTAGGCTTA+GGG | - | Chr1:36479191-36479210 | MsG0180002303.01.T01:CDS | 35.0% | |
| CCAAACTTTATATCCCTCTT+GGG | + | Chr1:36479691-36479710 | None:intergenic | 35.0% | |
| TACTATTATTGTTGCCCAAG+AGG | - | Chr1:36479674-36479693 | MsG0180002303.01.T01:CDS | 35.0% | |
| ! | AGTGTTGGAGAATCATACAA+TGG | - | Chr1:36479127-36479146 | MsG0180002303.01.T01:CDS | 35.0% |
| ! | GATCTCATTTGTTACTCTTG+GGG | - | Chr1:36479160-36479179 | MsG0180002303.01.T01:CDS | 35.0% |
| !! | AATCTTGTTTCTGTTGACTG+TGG | - | Chr1:36479459-36479478 | MsG0180002303.01.T01:CDS | 35.0% |
| !! | ACATTTGGTCTTGGTGAAAA+TGG | - | Chr1:36479366-36479385 | MsG0180002303.01.T01:CDS | 35.0% |
| !! | TCATACTTGTGTTGTTTCGT+CGG | - | Chr1:36479488-36479507 | MsG0180002303.01.T01:intron | 35.0% |
| !!! | TGAGGCTAAGTTTTTAGAAC+CGG | - | Chr1:36479626-36479645 | MsG0180002303.01.T01:CDS | 35.0% |
| AGAAGTGGTGTTCTTGGAAA+TGG | - | Chr1:36479726-36479745 | MsG0180002303.01.T01:CDS | 40.0% | |
| AGATGAAAATCTTCCACCAG+TGG | + | Chr1:36479795-36479814 | None:intergenic | 40.0% | |
| AGTCTTCGCAAGCAAGATAA+GGG | - | Chr1:36479834-36479853 | MsG0180002303.01.T01:CDS | 40.0% | |
| ATTTCACTCCAACACCAGTA+TGG | - | Chr1:36479041-36479060 | MsG0180002303.01.T01:CDS | 40.0% | |
| CCCAAGAGGGATATAAAGTT+TGG | - | Chr1:36479688-36479707 | MsG0180002303.01.T01:CDS | 40.0% | |
| CGATAGACCAAATCCAGAAA+TGG | - | Chr1:36479222-36479241 | MsG0180002303.01.T01:CDS | 40.0% | |
| CTTGGTGAAAATGGACAACT+TGG | - | Chr1:36479375-36479394 | MsG0180002303.01.T01:CDS | 40.0% | |
| GAAAATGGACAACTTGGTCA+TGG | - | Chr1:36479381-36479400 | MsG0180002303.01.T01:CDS | 40.0% | |
| GGCAATTCTTGAACAAGTTC+AGG | + | Chr1:36479429-36479448 | None:intergenic | 40.0% | |
| TCATGGTCAATTAGGCTTAG+GGG | - | Chr1:36479192-36479211 | MsG0180002303.01.T01:CDS | 40.0% | |
| TCTTGGGGTAACAACAATCA+TGG | - | Chr1:36479175-36479194 | MsG0180002303.01.T01:CDS | 40.0% | |
| TGTCCACATCAAAGCAAAGA+AGG | - | Chr1:36478998-36479017 | MsG0180002303.01.T01:CDS | 40.0% | |
| TTTCACTCCAACACCAGTAT+GGG | - | Chr1:36479042-36479061 | MsG0180002303.01.T01:CDS | 40.0% | |
| ! | AAAGTTTGGTCTTTCGGAAG+AGG | - | Chr1:36479702-36479721 | MsG0180002303.01.T01:CDS | 40.0% |
| ! | ATACTGGTGTTGGAGTGAAA+TGG | + | Chr1:36479042-36479061 | None:intergenic | 40.0% |
| ! | CTTTTACTACTGTGTGACCA+TGG | + | Chr1:36479072-36479091 | None:intergenic | 40.0% |
| ! | GAAAGTGTGTGTTGGACATT+TGG | - | Chr1:36479351-36479370 | MsG0180002303.01.T01:CDS | 40.0% |
| ACACCAGTATGGGATTTCCA+TGG | - | Chr1:36479052-36479071 | MsG0180002303.01.T01:CDS | 45.0% | |
| AGAGGAAGAAGTGGTGTTCT+TGG | - | Chr1:36479720-36479739 | MsG0180002303.01.T01:CDS | 45.0% | |
| GAGTCTTCGCAAGCAAGATA+AGG | - | Chr1:36479833-36479852 | MsG0180002303.01.T01:CDS | 45.0% | |
| GGAGGCCATAACACTAAAGT+GGG | + | Chr1:36479774-36479793 | None:intergenic | 45.0% | |
| GTGTGTTGGACATTTGGTCT+TGG | - | Chr1:36479357-36479376 | MsG0180002303.01.T01:CDS | 45.0% | |
| TCAATTAGGCTTAGGGGACA+CGG | - | Chr1:36479198-36479217 | MsG0180002303.01.T01:CDS | 45.0% | |
| TCTTTCGGAAGAGGAAGAAG+TGG | - | Chr1:36479711-36479730 | MsG0180002303.01.T01:CDS | 45.0% | |
| TGAAAATCTTCCACCAGTGG+AGG | + | Chr1:36479792-36479811 | None:intergenic | 45.0% | |
| TGACCATGGAAATCCCATAC+TGG | + | Chr1:36479058-36479077 | None:intergenic | 45.0% | |
| TGGAAATCCCATACTGGTGT+TGG | + | Chr1:36479052-36479071 | None:intergenic | 45.0% | |
| TGGAGGCCATAACACTAAAG+TGG | + | Chr1:36479775-36479794 | None:intergenic | 45.0% | |
| TTTAGTGTTATGGCCTCCAC+TGG | - | Chr1:36479776-36479795 | MsG0180002303.01.T01:CDS | 45.0% | |
| !! | ACTTGTGTTGTTTCGTCGGA+CGG | - | Chr1:36479492-36479511 | MsG0180002303.01.T01:intron | 45.0% |
| !! | GAAGGGTCTTGGTTTGTATC+CGG | - | Chr1:36479539-36479558 | MsG0180002303.01.T01:intron | 45.0% |
| TCTTGCTTGCGAAGACTCGA+TGG | + | Chr1:36479831-36479850 | None:intergenic | 50.0% | |
| !! | CTTGTGTTGTTTCGTCGGAC+GGG | - | Chr1:36479493-36479512 | MsG0180002303.01.T01:intron | 50.0% |
| !! | GAACCGGTTCAAGTTGCATG+TGG | - | Chr1:36479642-36479661 | MsG0180002303.01.T01:CDS | 50.0% |
| !! | TTGTGTTGTTTCGTCGGACG+GGG | - | Chr1:36479494-36479513 | MsG0180002303.01.T01:intron | 50.0% |
| AGTGTTATGGCCTCCACTGG+TGG | - | Chr1:36479779-36479798 | MsG0180002303.01.T01:CDS | 55.0% | |
| GCGCCACATGCAACTTGAAC+CGG | + | Chr1:36479648-36479667 | None:intergenic | 55.0% | |
| GGATGTTTGGTCGTGGGGAA+TGG | - | Chr1:36479515-36479534 | MsG0180002303.01.T01:intron | 55.0% | |
| TATCCGGATGCGCGAGTTGT+TGG | - | Chr1:36479555-36479574 | MsG0180002303.01.T01:intron | 55.0% | |
| TCACCAACAACTCGCGCATC+CGG | + | Chr1:36479561-36479580 | None:intergenic | 55.0% | |
| TGGGGAATGGAGAAGGGTCT+TGG | - | Chr1:36479528-36479547 | MsG0180002303.01.T01:intron | 55.0% | |
| TGGTCGTGGGGAATGGAGAA+GGG | - | Chr1:36479522-36479541 | MsG0180002303.01.T01:intron | 55.0% | |
| TTGGTCGTGGGGAATGGAGA+AGG | - | Chr1:36479521-36479540 | MsG0180002303.01.T01:intron | 55.0% | |
| TTTCGTCGGACGGGGATGTT+TGG | - | Chr1:36479502-36479521 | MsG0180002303.01.T01:intron | 55.0% | |
| !! | GGTTCAAGTTGCATGTGGCG+CGG | - | Chr1:36479647-36479666 | MsG0180002303.01.T01:CDS | 55.0% |
| GACGGGGATGTTTGGTCGTG+GGG | - | Chr1:36479510-36479529 | MsG0180002303.01.T01:intron | 60.0% | |
| GGACGGGGATGTTTGGTCGT+GGG | - | Chr1:36479509-36479528 | MsG0180002303.01.T01:intron | 60.0% | |
| CGGACGGGGATGTTTGGTCG+TGG | - | Chr1:36479508-36479527 | MsG0180002303.01.T01:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 36478983 | 36479872 | 36478983 | ID=MsG0180002303.01;Name=MsG0180002303.01 |
| Chr1 | mRNA | 36478983 | 36479872 | 36478983 | ID=MsG0180002303.01.T01;Parent=MsG0180002303.01;Name=MsG0180002303.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|269 |
| Chr1 | exon | 36479584 | 36479872 | 36479584 | ID=MsG0180002303.01.T01:exon:153;Parent=MsG0180002303.01.T01 |
| Chr1 | exon | 36478983 | 36479503 | 36478983 | ID=MsG0180002303.01.T01:exon:154;Parent=MsG0180002303.01.T01 |
| Chr1 | CDS | 36479584 | 36479872 | 36479584 | ID=MsG0180002303.01.T01:cds;Parent=MsG0180002303.01.T01 |
| Chr1 | CDS | 36478983 | 36479503 | 36478983 | ID=MsG0180002303.01.T01:cds;Parent=MsG0180002303.01.T01 |
| Gene Sequence |
| Protein sequence |
