AlfalfaGEDB | MsG0180002322.01

M. sativa cultivar ZhongmuNo.1 / MsG0180002322.01

KEGG: -
GO: GO:0006486 GO:0016757
PFAM: PF03016

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002322.01.T01	XP_003588379.2	91.176	612	29	3	1	611	1	588	0	1144

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002322.01.T01	Q9FFN2	44.557	395	205	6	222	606	121	511	1.43E-107	336

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002322.01.T01	G7IB43	91.176	612	29	3	1	611	1	588	0.0	1144

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000869.01	MsG0180002322.01	0.918660	1.184488e-86	3.648442e-82
MsG0180002322.01	MsG0280010942.01	0.818122	2.311467e-52	1.715186e-49
MsG0180002322.01	MsG0580029897.01	0.801061	1.075694e-48	5.088726e-46
MsG0180002322.01	MsG0680031334.01	0.809339	1.986845e-50	1.163482e-47
MsG0180002322.01	MsG0680031692.01	0.804540	2.056905e-49	1.063312e-46
MsG0180002322.01	MsG0880046471.01	0.806819	6.835239e-50	3.746509e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002322.01.T01	MTR_1g006600	91.176	612	29	3	1	611	1	588	0.0	1144
MsG0180002322.01.T01	MTR_3g110198	63.520	625	184	9	1	611	1	595	0.0	774
MsG0180002322.01.T01	MTR_7g103150	75.176	427	105	1	185	611	61	486	0.0	692
MsG0180002322.01.T01	MTR_8g102840	49.918	613	287	8	5	607	5	607	0.0	586
MsG0180002322.01.T01	MTR_3g110200	47.444	626	267	10	3	607	4	588	0.0	579
MsG0180002322.01.T01	MTR_1g090733	64.402	368	125	2	240	606	182	544	3.94e-177	513
MsG0180002322.01.T01	MTR_4g100730	60.215	372	142	4	241	606	161	532	2.71e-161	473
MsG0180002322.01.T01	MTR_8g102840	45.731	527	266	8	5	521	5	521	1.90e-148	439
MsG0180002322.01.T01	MTR_7g103260	45.573	384	195	5	233	606	14	393	7.53e-115	348
MsG0180002322.01.T01	MTR_3g097310	44.063	379	197	4	239	606	113	487	9.71e-113	346

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002322.01.T01	AT5G25820	51.156	649	262	11	8	607	8	650	0.0	651
MsG0180002322.01.T01	AT4G32790	52.101	595	250	10	15	607	30	591	0.0	605
MsG0180002322.01.T01	AT4G32790	52.101	595	250	10	15	607	30	591	0.0	605
MsG0180002322.01.T01	AT4G32790	52.101	595	250	10	15	607	30	591	0.0	605
MsG0180002322.01.T01	AT5G19670	66.337	404	135	1	207	609	204	607	0.0	593
MsG0180002322.01.T01	AT5G19670	66.337	404	135	1	207	609	204	607	0.0	593
MsG0180002322.01.T01	AT5G11610	55.063	474	197	8	148	609	76	545	0.0	530
MsG0180002322.01.T01	AT5G37000	57.506	433	178	3	175	606	121	548	4.36e-180	521
MsG0180002322.01.T01	AT4G16745	59.189	370	145	4	241	604	157	526	1.07e-158	466
MsG0180002322.01.T01	AT4G16745	57.778	360	146	4	241	594	157	516	4.88e-147	438

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Find 121 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAGATGAGCCTTCTTT+GGG	0.402028	1:+36711786	None:intergenic
AAAAGATAAAGAACAAATCT+TGG	0.423927	1:-36713746	MsG0180002322.01.T01:five_prime_UTR
AAAATCGCTTCCACGACTCT+TGG	0.467339	1:+36710498	None:intergenic
AAAATTGGAAGTTCCTTCAA+CGG	0.575938	1:+36713853	None:intergenic
AAACGCTCAAATTCCAACAA+GGG	0.666050	1:-36710742	MsG0180002322.01.T01:CDS
AAAGGTGGACTTAAATTTCT+TGG	0.262697	1:+36712737	None:intergenic
AAAGTGTATGTATACAGAGA+AGG	0.623220	1:-36711911	MsG0180002322.01.T01:CDS
AAAGTGTTTCACAATCAAAG+TGG	0.614307	1:+36713975	None:intergenic
AAATATATTACTTTCTATTC+CGG	0.268971	1:-36710361	MsG0180002322.01.T01:CDS
AAATTATCAGATATAATAAC+CGG	0.469771	1:+36710462	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATTGGAAGTTCCTTCAA+CGG	+	Chr1:36710092-36710111	None:intergenic	30.0%
AAAGTGTATGTATACAGAGA+AGG	-	Chr1:36712031-36712050	MsG0180002322.01.T01:intron	30.0%
AACACTGCAGTTGTAATATT+CGG	+	Chr1:36711250-36711269	None:intergenic	30.0%
ACTGCCTTAAACTGTTAAAA+CGG	-	Chr1:36711859-36711878	MsG0180002322.01.T01:CDS	30.0%
AGAGAAAGTTAATGCAACTA+TGG	-	Chr1:36711027-36711046	MsG0180002322.01.T01:intron	30.0%
AGTACTGTATTTGTGCAAAA+GGG	-	Chr1:36713406-36713425	MsG0180002322.01.T01:intron	30.0%
AGTGATTGTATGTAATGTGT+CGG	+	Chr1:36713997-36714016	None:intergenic	30.0%
AGTTGCATTAACTTTCTCTA+TGG	+	Chr1:36711026-36711045	None:intergenic	30.0%
ATAATGTTTCCATGTTCAAG+AGG	-	Chr1:36711670-36711689	MsG0180002322.01.T01:CDS	30.0%
ATATGATCCTTCACTCTATT+TGG	-	Chr1:36713694-36713713	MsG0180002322.01.T01:five_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	36709870	36714094	36709870	ID=MsG0180002322.01;Name=MsG0180002322.01
Chr1	mRNA	36709870	36714094	36709870	ID=MsG0180002322.01.T01;Parent=MsG0180002322.01;Name=MsG0180002322.01.T01;_AED=0.47;_eAED=0.47;_QI=467\|1\|1\|1\|0.5\|0.6\|5\|348\|611
Chr1	exon	36713682	36714094	36713682	ID=MsG0180002322.01.T01:exon:420;Parent=MsG0180002322.01.T01
Chr1	exon	36712484	36713230	36712484	ID=MsG0180002322.01.T01:exon:421;Parent=MsG0180002322.01.T01
Chr1	exon	36712273	36712403	36712273	ID=MsG0180002322.01.T01:exon:422;Parent=MsG0180002322.01.T01
Chr1	exon	36711613	36711961	36711613	ID=MsG0180002322.01.T01:exon:423;Parent=MsG0180002322.01.T01
Chr1	exon	36709870	36710880	36709870	ID=MsG0180002322.01.T01:exon:424;Parent=MsG0180002322.01.T01
Chr1	five_prime_UTR	36713682	36714094	36713682	ID=MsG0180002322.01.T01:five_prime_utr;Parent=MsG0180002322.01.T01
Chr1	five_prime_UTR	36713177	36713230	36713177	ID=MsG0180002322.01.T01:five_prime_utr;Parent=MsG0180002322.01.T01
Chr1	CDS	36712484	36713176	36712484	ID=MsG0180002322.01.T01:cds;Parent=MsG0180002322.01.T01
Chr1	CDS	36712273	36712403	36712273	ID=MsG0180002322.01.T01:cds;Parent=MsG0180002322.01.T01
Chr1	CDS	36711613	36711961	36711613	ID=MsG0180002322.01.T01:cds;Parent=MsG0180002322.01.T01
Chr1	CDS	36710218	36710880	36710218	ID=MsG0180002322.01.T01:cds;Parent=MsG0180002322.01.T01
Chr1	three_prime_UTR	36709870	36710217	36709870	ID=MsG0180002322.01.T01:three_prime_utr;Parent=MsG0180002322.01.T01

Gene Sequence

>MsG0180002322.01.T01

ATGGGTGGAGATTTTTTGTCAATGTTTCAGCTAGAAACAAAGAGATTAATATGGCTTATTGGCATTACTTTTTCAATAATCTTTGCTTTTCAGTATGTTGAACCAAATTATGGCAATGTTCTATTCTCTCTATTCTCTGCTGATAAAATACCAATATCATCAGAAAGTACAATATCTCAAGCTACAGATACCAATGTCACAATTTTGAATCATGCAAACTTTACAGATGAACTTTCCATAGAGAAAGTTAATGCAACTATGGATACTGTTTCAATAACTGGTTTTGTCTTAGAACCAGAATTGTCACAAAACAAATCTCAAGGATTTGATAAGTCTCAAGAAAATAGTGTATCGATATCAAGACATGAACATAAATCTGAAAGACTAGATTCTCATTCTCCTTCTCCTGAGAATGCACCAAGAAATTTAAGTCCACCTTTTGGTTCTACAAGTGTCGGTCCGAATATTACAACTGCAGTGTTTTCAAAGGATGATAACATAACAAATTCGATAAAGAAAGAGAGTTTTAGTCCATCGTCACAGAATGGTGGCAATATACAAGACAAGAGTTCTTCTATCAGCAGCACTGTGCCTAAGGAGCGTCAAGAAATTCATGCACCGATTAAAGAAGTCACGACAGTGTCTGAAATGAACAAGTTATTGCTTGAAAGCCATGCTTCGTATCGATCAATGAAGCCAAGGTGGTCTTCAAATGTTGATCAAGAGTTACTAGAGGCAAGATCAGAGATTGAAAATGCACCAATTGTAAAGAATGATCCAAATCTTTATGGTCCAATTTATCATAATGTTTCCATGTTCAAGAGGAGCTATGAATTAATGGAAGAGAGACTCAAAGTGTATGTATACAGAGAAGGAGCTAGACCTATACTGCATTCGCCGTTTCTCACCGGAATCTATGCTTCTGAAGGGTGGTTTATGAAGCTAATGGAAGCTAATAAACATTTTGTTACCAAAAACCCAAAGAAGGCTCATCTGTTTTACTTACCTTTCAGTTCTAGGATGCTAGAGGAAGCCTTGTATGTGAAGAATTCGCATAGCCACAAGAACTTGATTCAATATCTACATCATTATGTAGACCTGATTGCAGCGAGACATTCTTTCTGGAACAGAACTGGAGGTGCTGATCATTTTCTAGTTGGTTGTCATGATTGGGCTCCATCGGAAACAAAATTACGCTTGGCAAACTGCATAAGGTCCCTCTGCAACGCCGATGTAAAAGAAGGATTTGTCTTCGGCAAAGACGCGTCTCTTCCTGAAACATACGTCCGAAACGCTCAAATTCCAACAAGGGATCTTGGTGGCAATTCATTTTCAAAGAAGACAACATTAGCCTTTTTCGCAGGGAGCATGCATGGTTATGTAAGGCCAATACTATTGAAACATTGGGAGAACAAAGATCCTGACATGAAAATCTTTGGAAAATTGCCAAAGGTTAAAGGAAATAGTAACTACATTCATTACATGAAGAGTAGCAAGTACTGTATTTGTGCAAAAGGGTATGAAGTGAATAGTCCAAGAGTCGTGGAAGCGATTTTCTACGAATGTGTTCCGGTTATTATATCTGATAATTTTGTGCCACCCTTTTTCGAGGTTTTGGATTGGGAATCCTTTTCTGTCATTGTTCTGGAGAAGGATATTCCAAATTTGAAAAATATATTACTTTCTATTCCGGAAAAGAGGTATCTTAGCTTGCTAATGAGGGTAAAAAAGGTACAGAAGCATTTCCTTTGGAACAAGAATCCTGTCAAGTATGATATATTTCATATGATCCTTCACTCTATTTGGTACAATAGAGTCTTCTCTGCCACTAGCTAG

Protein sequence

>MsG0180002322.01.T01

MGGDFLSMFQLETKRLIWLIGITFSIIFAFQYVEPNYGNVLFSLFSADKIPISSESTISQATDTNVTILNHANFTDELSIEKVNATMDTVSITGFVLEPELSQNKSQGFDKSQENSVSISRHEHKSERLDSHSPSPENAPRNLSPPFGSTSVGPNITTAVFSKDDNITNSIKKESFSPSSQNGGNIQDKSSSISSTVPKERQEIHAPIKEVTTVSEMNKLLLESHASYRSMKPRWSSNVDQELLEARSEIENAPIVKNDPNLYGPIYHNVSMFKRSYELMEERLKVYVYREGARPILHSPFLTGIYASEGWFMKLMEANKHFVTKNPKKAHLFYLPFSSRMLEEALYVKNSHSHKNLIQYLHHYVDLIAARHSFWNRTGGADHFLVGCHDWAPSETKLRLANCIRSLCNADVKEGFVFGKDASLPETYVRNAQIPTRDLGGNSFSKKTTLAFFAGSMHGYVRPILLKHWENKDPDMKIFGKLPKVKGNSNYIHYMKSSKYCICAKGYEVNSPRVVEAIFYECVPVIISDNFVPPFFEVLDWESFSVIVLEKDIPNLKNILLSIPEKRYLSLLMRVKKVQKHFLWNKNPVKYDIFHMILHSIWYNRVFSATS*