Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002362.01.T01 | XP_024641592.1 | 63.918 | 97 | 23 | 1 | 1 | 97 | 39 | 123 | 1.59E-29 | 112 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002362.01.T01 | A0A2Z6MMY9 | 42.683 | 82 | 40 | 1 | 23 | 97 | 2 | 83 | 1.41e-11 | 69.7 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000866.01 | MsG0180002362.01 | 0.889386 | 2.538534e-73 | 2.043656e-69 |
| MsG0180002362.01 | MsG0180005019.01 | 0.876045 | 1.906734e-68 | 9.109086e-65 |
| MsG0180002362.01 | MsG0480020367.01 | 0.856601 | 2.882258e-62 | 6.916844e-59 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTGTTCTAGCAACTTGAAT+TGG | 0.324721 | 1:-37378797 | None:intergenic |
| TGCACTAACTCAGCATGTTC+AGG | 0.335036 | 1:-37378857 | None:intergenic |
| GCAACTTGAATTGGAATTTG+AGG | 0.378140 | 1:-37378788 | None:intergenic |
| TGTTTAAGCATGTTGTTAGT+TGG | 0.419688 | 1:-37379004 | None:intergenic |
| CTAGACATCCTTCAATGGCT+TGG | 0.481979 | 1:+37378903 | MsG0180002362.01.T01:CDS |
| AAATGTCTCCAAGCCATTGA+AGG | 0.489069 | 1:-37378911 | None:intergenic |
| AGTGAAACAGAAGTTACAAA+AGG | 0.500447 | 1:-37378954 | None:intergenic |
| AGATGCTAGACATCCTTCAA+TGG | 0.529205 | 1:+37378898 | MsG0180002362.01.T01:CDS |
| TAAGCATGTTGTTAGTTGGA+TGG | 0.563365 | 1:-37379000 | None:intergenic |
| TGCTAGAACACAAGAAGTTG+AGG | 0.602315 | 1:+37378808 | MsG0180002362.01.T01:CDS |
| GAGGCTGCTGAGTTAGTACA+TGG | 0.612193 | 1:+37378827 | MsG0180002362.01.T01:CDS |
| AGGAAAATGATTCACACTAG+TGG | 0.616118 | 1:-37378768 | None:intergenic |
| GCTGAGTTAGTACATGGACA+AGG | 0.720148 | 1:+37378833 | MsG0180002362.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTCACTTCTTTTATGTCAAA+TGG | + | Chr1:37378971-37378990 | MsG0180002362.01.T01:CDS | 25.0% |
| AGTGAAACAGAAGTTACAAA+AGG | - | Chr1:37378957-37378976 | None:intergenic | 30.0% | |
| TGTTTAAGCATGTTGTTAGT+TGG | - | Chr1:37379007-37379026 | None:intergenic | 30.0% | |
| AGGAAAATGATTCACACTAG+TGG | - | Chr1:37378771-37378790 | None:intergenic | 35.0% | |
| GCAACTTGAATTGGAATTTG+AGG | - | Chr1:37378791-37378810 | None:intergenic | 35.0% | |
| TAAGCATGTTGTTAGTTGGA+TGG | - | Chr1:37379003-37379022 | None:intergenic | 35.0% | |
| TGTGTTCTAGCAACTTGAAT+TGG | - | Chr1:37378800-37378819 | None:intergenic | 35.0% | |
| AAATGTCTCCAAGCCATTGA+AGG | - | Chr1:37378914-37378933 | None:intergenic | 40.0% | |
| AGATGCTAGACATCCTTCAA+TGG | + | Chr1:37378898-37378917 | MsG0180002362.01.T01:CDS | 40.0% | |
| TGCTAGAACACAAGAAGTTG+AGG | + | Chr1:37378808-37378827 | MsG0180002362.01.T01:CDS | 40.0% | |
| CTAGACATCCTTCAATGGCT+TGG | + | Chr1:37378903-37378922 | MsG0180002362.01.T01:CDS | 45.0% | |
| GCTGAGTTAGTACATGGACA+AGG | + | Chr1:37378833-37378852 | MsG0180002362.01.T01:CDS | 45.0% | |
| TGCACTAACTCAGCATGTTC+AGG | - | Chr1:37378860-37378879 | None:intergenic | 45.0% | |
| GAGGCTGCTGAGTTAGTACA+TGG | + | Chr1:37378827-37378846 | MsG0180002362.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 37378755 | 37379051 | 37378755 | ID=MsG0180002362.01;Name=MsG0180002362.01 |
| Chr1 | mRNA | 37378755 | 37379051 | 37378755 | ID=MsG0180002362.01.T01;Parent=MsG0180002362.01;Name=MsG0180002362.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|98 |
| Chr1 | exon | 37378755 | 37379051 | 37378755 | ID=MsG0180002362.01.T01:exon:43;Parent=MsG0180002362.01.T01 |
| Chr1 | CDS | 37378755 | 37379051 | 37378755 | ID=MsG0180002362.01.T01:cds;Parent=MsG0180002362.01.T01 |
| Gene Sequence |
| Protein sequence |
