Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000866.01 | MsG0180005019.01 | 0.875767 | 2.376879e-68 | 1.123542e-64 |
| MsG0180002362.01 | MsG0180005019.01 | 0.876045 | 1.906734e-68 | 9.109086e-65 |
| MsG0180005019.01 | MsG0480020367.01 | 0.817596 | 3.037845e-52 | 2.221891e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 6 sgRNAs with CRISPR-Local
Find 8 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTTAAGCATGTTGTTAGT+TGG | 0.422483 | 1:+85727624 | None:intergenic |
| CTAGACATCCTTCAATGGCT+TGG | 0.481979 | 1:-85727725 | MsG0180005019.01.T01:CDS |
| AAATGTCTCCAAGCCATTGA+AGG | 0.489069 | 1:+85727717 | None:intergenic |
| AGTGAAACAGAAGTTACAAA+AGG | 0.500447 | 1:+85727674 | None:intergenic |
| AGATGCTAGACATCCTTCAA+TGG | 0.529205 | 1:-85727730 | MsG0180005019.01.T01:CDS |
| TAAGCATGTTGTTAGTTGGA+TGG | 0.563365 | 1:+85727628 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAAACTACCTTTATCTTTT+TGG | + | Chr1:85727701-85727720 | None:intergenic | 20.0% |
| ! | TGATATGTCCAAAAAGATAA+AGG | - | Chr1:85727690-85727709 | MsG0180005019.01.T01:CDS | 25.0% |
| !! | TTCACTTCTTTTATGTCAAA+TGG | - | Chr1:85727630-85727649 | MsG0180005019.01.T01:CDS | 25.0% |
| AGTGAAACAGAAGTTACAAA+AGG | + | Chr1:85727616-85727635 | None:intergenic | 30.0% | |
| TGTTTAAGCATGTTGTTAGT+TGG | + | Chr1:85727666-85727685 | None:intergenic | 30.0% | |
| TAAGCATGTTGTTAGTTGGA+TGG | + | Chr1:85727662-85727681 | None:intergenic | 35.0% | |
| AAATGTCTCCAAGCCATTGA+AGG | + | Chr1:85727573-85727592 | None:intergenic | 40.0% | |
| CTAGACATCCTTCAATGGCT+TGG | - | Chr1:85727562-85727581 | MsG0180005019.01.T01:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 85727559 | 85727750 | 85727559 | ID=MsG0180005019.01;Name=MsG0180005019.01 |
| Chr1 | mRNA | 85727559 | 85727750 | 85727559 | ID=MsG0180005019.01.T01;Parent=MsG0180005019.01;Name=MsG0180005019.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|0|1|0|63 |
| Chr1 | exon | 85727559 | 85727750 | 85727559 | ID=MsG0180005019.01.T01:exon:9736;Parent=MsG0180005019.01.T01 |
| Chr1 | CDS | 85727559 | 85727750 | 85727559 | ID=MsG0180005019.01.T01:cds;Parent=MsG0180005019.01.T01 |
| Gene Sequence |
| Protein sequence |
