Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020367.01.T01 | XP_024641592.1 | 60.825 | 97 | 26 | 1 | 1 | 97 | 39 | 123 | 2.60E-26 | 104 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002362.01 | MsG0480020367.01 | 0.856601 | 2.882258e-62 | 6.916844e-59 |
| MsG0180005019.01 | MsG0480020367.01 | 0.817596 | 3.037845e-52 | 2.221891e-49 |
| MsG0280006954.01 | MsG0480020367.01 | 0.882512 | 9.792205e-71 | 5.981517e-67 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTGTTCTAGCAACTTGAAT+TGG | 0.324721 | 4:+39873673 | None:intergenic |
| TGCACTAACTCAGCATGTTC+AGG | 0.335036 | 4:+39873613 | None:intergenic |
| GCAACTTGAATTGGAATTTG+AGG | 0.378140 | 4:+39873682 | None:intergenic |
| ATATGTTTAAGCATGTTGTT+AGG | 0.426589 | 4:+39873463 | None:intergenic |
| ACTCAGCATGTTCAGGTCCT+TGG | 0.469300 | 4:+39873620 | None:intergenic |
| CTAGACATCCTTCAATGGCT+TGG | 0.481979 | 4:-39873567 | MsG0480020367.01.T01:CDS |
| AAATGTCTCCAAGCCATTGA+AGG | 0.489069 | 4:+39873559 | None:intergenic |
| AGTGAAACAGAAGTTACAAA+AGG | 0.500447 | 4:+39873516 | None:intergenic |
| AGATGCTAGACATCCTTCAA+TGG | 0.529205 | 4:-39873572 | MsG0480020367.01.T01:CDS |
| TAAGCATGTTGTTAGGTGGA+TGG | 0.572076 | 4:+39873470 | None:intergenic |
| TGTTTAAGCATGTTGTTAGG+TGG | 0.597759 | 4:+39873466 | None:intergenic |
| TGCTAGAACACAAGAAGTTG+AGG | 0.602315 | 4:-39873662 | MsG0480020367.01.T01:CDS |
| AGGAAAATGATTCACACTAG+TGG | 0.616118 | 4:+39873702 | None:intergenic |
| GCTGAGTTAGTACATAGCCA+AGG | 0.681772 | 4:-39873637 | MsG0480020367.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCTTTTATGTCAAATAGTTT+TGG | - | Chr4:39873663-39873682 | MsG0480020367.01.T01:CDS | 20.0% |
| ! | ATATGTTTAAGCATGTTGTT+AGG | + | Chr4:39873696-39873715 | None:intergenic | 25.0% |
| AGTGAAACAGAAGTTACAAA+AGG | + | Chr4:39873643-39873662 | None:intergenic | 30.0% | |
| AGGAAAATGATTCACACTAG+TGG | + | Chr4:39873457-39873476 | None:intergenic | 35.0% | |
| GCAACTTGAATTGGAATTTG+AGG | + | Chr4:39873477-39873496 | None:intergenic | 35.0% | |
| TGTGTTCTAGCAACTTGAAT+TGG | + | Chr4:39873486-39873505 | None:intergenic | 35.0% | |
| TGTTTAAGCATGTTGTTAGG+TGG | + | Chr4:39873693-39873712 | None:intergenic | 35.0% | |
| AAATGTCTCCAAGCCATTGA+AGG | + | Chr4:39873600-39873619 | None:intergenic | 40.0% | |
| AGATGCTAGACATCCTTCAA+TGG | - | Chr4:39873584-39873603 | MsG0480020367.01.T01:CDS | 40.0% | |
| TAAGCATGTTGTTAGGTGGA+TGG | + | Chr4:39873689-39873708 | None:intergenic | 40.0% | |
| TGCTAGAACACAAGAAGTTG+AGG | - | Chr4:39873494-39873513 | MsG0480020367.01.T01:CDS | 40.0% | |
| CTAGACATCCTTCAATGGCT+TGG | - | Chr4:39873589-39873608 | MsG0480020367.01.T01:CDS | 45.0% | |
| GCTGAGTTAGTACATAGCCA+AGG | - | Chr4:39873519-39873538 | MsG0480020367.01.T01:CDS | 45.0% | |
| TGCACTAACTCAGCATGTTC+AGG | + | Chr4:39873546-39873565 | None:intergenic | 45.0% | |
| ACTCAGCATGTTCAGGTCCT+TGG | + | Chr4:39873539-39873558 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 39873441 | 39873737 | 39873441 | ID=MsG0480020367.01;Name=MsG0480020367.01 |
| Chr4 | mRNA | 39873441 | 39873737 | 39873441 | ID=MsG0480020367.01.T01;Parent=MsG0480020367.01;Name=MsG0480020367.01.T01;_AED=0.24;_eAED=0.24;_QI=0|-1|0|1|-1|1|1|0|98 |
| Chr4 | exon | 39873441 | 39873737 | 39873441 | ID=MsG0480020367.01.T01:exon:16778;Parent=MsG0480020367.01.T01 |
| Chr4 | CDS | 39873441 | 39873737 | 39873441 | ID=MsG0480020367.01.T01:cds;Parent=MsG0480020367.01.T01 |
| Gene Sequence |
| Protein sequence |
