Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002385.01.T01 | KEH17594.1 | 82.682 | 179 | 31 | 0 | 52 | 230 | 88 | 266 | 6.60E-91 | 297 |
| MsG0180002385.01.T01 | KEH17594.1 | 31.383 | 188 | 114 | 6 | 55 | 232 | 592 | 774 | 6.03E-12 | 77 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002385.01.T01 | A0A072TJ50 | 82.682 | 179 | 31 | 0 | 52 | 230 | 88 | 266 | 3.15e-91 | 297 |
| MsG0180002385.01.T01 | A0A396JL51 | 84.270 | 178 | 28 | 0 | 53 | 230 | 1017 | 1194 | 1.87e-90 | 301 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002385.01 | MsG0180002602.01 | 0.906062 | 2.215876e-80 | 3.690290e-76 |
| MsG0180002385.01 | MsG0280007331.01 | 0.812540 | 4.027696e-51 | 2.567885e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002385.01.T01 | MTR_0006s0160 | 82.682 | 179 | 31 | 0 | 52 | 230 | 88 | 266 | 7.99e-95 | 297 |
| MsG0180002385.01.T01 | MTR_0006s0160 | 31.383 | 188 | 114 | 6 | 55 | 232 | 592 | 774 | 7.30e-16 | 77.0 |
| MsG0180002385.01.T01 | MTR_0069s0040 | 84.659 | 176 | 27 | 0 | 55 | 230 | 1018 | 1193 | 7.96e-94 | 301 |
| MsG0180002385.01.T01 | MTR_1g037130 | 83.240 | 179 | 30 | 0 | 52 | 230 | 1718 | 1896 | 3.56e-93 | 299 |
| MsG0180002385.01.T01 | MTR_1g037130 | 80.447 | 179 | 35 | 0 | 52 | 230 | 1014 | 1192 | 6.58e-90 | 290 |
| MsG0180002385.01.T01 | MTR_0006s0230 | 83.240 | 179 | 30 | 0 | 52 | 230 | 1689 | 1867 | 4.81e-93 | 299 |
| MsG0180002385.01.T01 | MTR_0006s0230 | 79.888 | 179 | 36 | 0 | 52 | 230 | 985 | 1163 | 1.37e-88 | 286 |
| MsG0180002385.01.T01 | MTR_0068s0080 | 82.022 | 178 | 32 | 0 | 53 | 230 | 1022 | 1199 | 2.46e-92 | 296 |
| MsG0180002385.01.T01 | MTR_0006s0300 | 81.006 | 179 | 34 | 0 | 52 | 230 | 54 | 232 | 3.34e-92 | 283 |
| MsG0180002385.01.T01 | MTR_1g051585 | 83.523 | 176 | 29 | 0 | 55 | 230 | 1018 | 1193 | 2.88e-91 | 294 |
| MsG0180002385.01.T01 | MTR_1g051585 | 31.383 | 188 | 114 | 6 | 55 | 232 | 1514 | 1696 | 3.08e-17 | 80.9 |
| MsG0180002385.01.T01 | MTR_0068s0080 | 81.564 | 179 | 33 | 0 | 52 | 230 | 1012 | 1190 | 3.78e-91 | 293 |
| MsG0180002385.01.T01 | MTR_1g036800 | 81.006 | 179 | 34 | 0 | 52 | 230 | 1013 | 1191 | 6.33e-91 | 293 |
| MsG0180002385.01.T01 | MTR_1g037150 | 82.955 | 176 | 30 | 0 | 55 | 230 | 1006 | 1181 | 9.62e-91 | 292 |
| MsG0180002385.01.T01 | MTR_1g037150 | 31.720 | 186 | 116 | 5 | 55 | 232 | 1517 | 1699 | 1.05e-15 | 76.6 |
| MsG0180002385.01.T01 | MTR_0006s0240 | 82.955 | 176 | 30 | 0 | 55 | 230 | 985 | 1160 | 1.26e-90 | 292 |
| MsG0180002385.01.T01 | MTR_0006s0240 | 35.326 | 184 | 108 | 5 | 55 | 230 | 1939 | 2119 | 3.20e-22 | 95.5 |
| MsG0180002385.01.T01 | MTR_0006s0240 | 31.551 | 187 | 117 | 5 | 55 | 233 | 1496 | 1679 | 8.95e-16 | 76.6 |
| MsG0180002385.01.T01 | MTR_0006s0080 | 79.558 | 181 | 37 | 0 | 52 | 232 | 838 | 1018 | 2.30e-89 | 288 |
| MsG0180002385.01.T01 | MTR_0006s0080 | 37.234 | 188 | 103 | 6 | 55 | 232 | 1352 | 1534 | 7.89e-22 | 94.4 |
| MsG0180002385.01.T01 | MTR_8g059345 | 81.818 | 176 | 32 | 0 | 55 | 230 | 1016 | 1191 | 2.58e-89 | 288 |
| MsG0180002385.01.T01 | MTR_2g089975 | 81.143 | 175 | 33 | 0 | 56 | 230 | 1019 | 1193 | 2.89e-89 | 288 |
| MsG0180002385.01.T01 | MTR_2g089975 | 36.702 | 188 | 104 | 6 | 55 | 232 | 2568 | 2750 | 2.93e-22 | 95.9 |
| MsG0180002385.01.T01 | MTR_2g089975 | 32.258 | 186 | 115 | 5 | 55 | 232 | 1529 | 1711 | 4.78e-16 | 77.4 |
| MsG0180002385.01.T01 | MTR_2g089975 | 32.821 | 195 | 114 | 8 | 55 | 239 | 2049 | 2236 | 4.81e-15 | 74.7 |
| MsG0180002385.01.T01 | MTR_1g037250 | 81.818 | 176 | 32 | 0 | 55 | 230 | 940 | 1115 | 4.70e-89 | 287 |
| MsG0180002385.01.T01 | MTR_1g037250 | 37.766 | 188 | 102 | 6 | 55 | 232 | 1372 | 1554 | 3.55e-22 | 95.5 |
| MsG0180002385.01.T01 | MTR_1g036750 | 79.888 | 179 | 36 | 0 | 52 | 230 | 985 | 1163 | 2.18e-88 | 285 |
| MsG0180002385.01.T01 | MTR_1g036750 | 37.234 | 188 | 103 | 6 | 55 | 232 | 2019 | 2201 | 6.37e-22 | 94.7 |
| MsG0180002385.01.T01 | MTR_1g036750 | 31.720 | 186 | 112 | 6 | 55 | 230 | 1499 | 1679 | 1.08e-15 | 76.6 |
| MsG0180002385.01.T01 | MTR_1g037010 | 78.771 | 179 | 38 | 0 | 52 | 230 | 940 | 1118 | 3.92e-88 | 285 |
| MsG0180002385.01.T01 | MTR_1g037010 | 38.624 | 189 | 98 | 7 | 55 | 232 | 1454 | 1635 | 3.95e-24 | 101 |
| MsG0180002385.01.T01 | MTR_6g464050 | 78.075 | 187 | 40 | 1 | 52 | 237 | 1631 | 1817 | 7.90e-88 | 284 |
| MsG0180002385.01.T01 | MTR_0012s0150 | 80.899 | 178 | 34 | 0 | 55 | 232 | 767 | 944 | 9.63e-87 | 280 |
| MsG0180002385.01.T01 | MTR_0012s0150 | 34.091 | 176 | 106 | 5 | 55 | 224 | 1278 | 1449 | 9.26e-16 | 76.6 |
| MsG0180002385.01.T01 | MTR_6g463150 | 77.401 | 177 | 40 | 0 | 54 | 230 | 1013 | 1189 | 5.81e-84 | 273 |
| MsG0180002385.01.T01 | MTR_6g463150 | 33.871 | 186 | 108 | 6 | 57 | 232 | 1523 | 1703 | 9.65e-18 | 82.4 |
| MsG0180002385.01.T01 | MTR_1g041550 | 77.841 | 176 | 25 | 1 | 55 | 230 | 1019 | 1180 | 5.78e-80 | 261 |
| MsG0180002385.01.T01 | MTR_1g041550 | 37.368 | 190 | 104 | 6 | 55 | 234 | 2036 | 2220 | 2.88e-23 | 98.6 |
| MsG0180002385.01.T01 | MTR_1g041550 | 32.308 | 195 | 115 | 7 | 55 | 239 | 1516 | 1703 | 5.80e-17 | 80.1 |
| MsG0180002385.01.T01 | MTR_1g044020 | 67.403 | 181 | 56 | 1 | 56 | 233 | 767 | 947 | 2.59e-72 | 239 |
| MsG0180002385.01.T01 | MTR_1g044020 | 34.211 | 190 | 109 | 5 | 55 | 234 | 2306 | 2489 | 1.90e-18 | 84.7 |
| MsG0180002385.01.T01 | MTR_1g044020 | 31.915 | 188 | 113 | 6 | 55 | 232 | 1279 | 1461 | 9.63e-17 | 79.7 |
| MsG0180002385.01.T01 | MTR_1g044020 | 31.383 | 188 | 114 | 6 | 55 | 232 | 1804 | 1986 | 1.00e-16 | 79.7 |
| MsG0180002385.01.T01 | MTR_1g044095 | 68.944 | 161 | 50 | 0 | 56 | 216 | 92 | 252 | 2.02e-69 | 230 |
| MsG0180002385.01.T01 | MTR_1g036520 | 67.614 | 176 | 25 | 1 | 55 | 230 | 596 | 739 | 2.57e-68 | 227 |
| MsG0180002385.01.T01 | MTR_1g442750 | 65.341 | 176 | 30 | 1 | 55 | 230 | 983 | 1127 | 8.35e-65 | 218 |
| MsG0180002385.01.T01 | MTR_1g442750 | 37.791 | 172 | 94 | 5 | 55 | 216 | 1869 | 2037 | 5.56e-21 | 92.0 |
| MsG0180002385.01.T01 | MTR_1g442750 | 32.275 | 189 | 115 | 6 | 53 | 232 | 1461 | 1645 | 1.56e-15 | 76.3 |
| MsG0180002385.01.T01 | MTR_0406s0030 | 65.000 | 160 | 27 | 1 | 52 | 211 | 941 | 1071 | 1.23e-54 | 189 |
| MsG0180002385.01.T01 | MTR_1g050402 | 58.824 | 170 | 68 | 2 | 55 | 223 | 1037 | 1205 | 2.93e-54 | 187 |
| MsG0180002385.01.T01 | MTR_1g050402 | 34.503 | 171 | 104 | 3 | 55 | 217 | 1549 | 1719 | 4.64e-15 | 74.7 |
| MsG0180002385.01.T01 | MTR_4g025470 | 47.399 | 173 | 87 | 2 | 61 | 232 | 999 | 1168 | 1.87e-40 | 148 |
| MsG0180002385.01.T01 | MTR_4g025430 | 46.154 | 182 | 94 | 2 | 50 | 230 | 955 | 1133 | 2.99e-38 | 141 |
| MsG0180002385.01.T01 | MTR_8g028500 | 34.591 | 159 | 98 | 2 | 57 | 209 | 1002 | 1160 | 2.11e-24 | 102 |
| MsG0180002385.01.T01 | MTR_8g028500 | 28.934 | 197 | 128 | 4 | 55 | 239 | 1505 | 1701 | 5.04e-17 | 80.5 |
| MsG0180002385.01.T01 | MTR_8g028500 | 30.675 | 163 | 98 | 2 | 63 | 210 | 2024 | 2186 | 2.52e-13 | 69.7 |
| MsG0180002385.01.T01 | MTR_2g071890 | 36.813 | 182 | 104 | 4 | 55 | 228 | 1006 | 1184 | 3.24e-24 | 101 |
| MsG0180002385.01.T01 | MTR_2g072820 | 34.946 | 186 | 110 | 4 | 55 | 232 | 1001 | 1183 | 1.04e-23 | 99.8 |
| MsG0180002385.01.T01 | MTR_1g051625 | 37.368 | 190 | 104 | 6 | 55 | 234 | 531 | 715 | 2.72e-23 | 98.6 |
| MsG0180002385.01.T01 | MTR_1g051625 | 33.511 | 188 | 112 | 6 | 53 | 232 | 9 | 191 | 4.86e-17 | 80.5 |
| MsG0180002385.01.T01 | MTR_6g464030 | 38.298 | 188 | 101 | 6 | 55 | 232 | 378 | 560 | 3.54e-23 | 98.2 |
| MsG0180002385.01.T01 | MTR_3g013790 | 38.509 | 161 | 91 | 3 | 57 | 212 | 1022 | 1179 | 5.07e-23 | 97.8 |
| MsG0180002385.01.T01 | MTR_2g072030 | 33.871 | 186 | 112 | 4 | 55 | 232 | 1038 | 1220 | 2.28e-22 | 95.9 |
| MsG0180002385.01.T01 | MTR_2g071860 | 32.692 | 208 | 122 | 6 | 37 | 232 | 541 | 742 | 4.93e-22 | 95.1 |
| MsG0180002385.01.T01 | MTR_8g059275 | 36.316 | 190 | 106 | 6 | 55 | 234 | 1458 | 1642 | 1.56e-21 | 93.6 |
| MsG0180002385.01.T01 | MTR_8g059275 | 32.979 | 188 | 111 | 6 | 55 | 232 | 546 | 728 | 1.93e-17 | 81.6 |
| MsG0180002385.01.T01 | MTR_8g059275 | 31.746 | 189 | 114 | 6 | 54 | 232 | 25 | 208 | 1.16e-15 | 76.3 |
| MsG0180002385.01.T01 | MTR_8g059275 | 28.384 | 229 | 148 | 7 | 14 | 232 | 898 | 1120 | 3.94e-15 | 74.7 |
| MsG0180002385.01.T01 | MTR_8g059275 | 36.316 | 190 | 106 | 6 | 55 | 234 | 1586 | 1770 | 1.71e-21 | 93.6 |
| MsG0180002385.01.T01 | MTR_8g059275 | 32.979 | 188 | 111 | 6 | 55 | 232 | 546 | 728 | 2.12e-17 | 81.6 |
| MsG0180002385.01.T01 | MTR_8g059275 | 32.979 | 188 | 111 | 6 | 55 | 232 | 1066 | 1248 | 2.12e-17 | 81.6 |
| MsG0180002385.01.T01 | MTR_8g059275 | 31.746 | 189 | 114 | 6 | 54 | 232 | 25 | 208 | 1.27e-15 | 76.3 |
| MsG0180002385.01.T01 | MTR_8g059275 | 36.316 | 190 | 106 | 6 | 55 | 234 | 1590 | 1774 | 1.72e-21 | 93.6 |
| MsG0180002385.01.T01 | MTR_8g059275 | 32.979 | 188 | 111 | 6 | 55 | 232 | 546 | 728 | 2.18e-17 | 81.6 |
| MsG0180002385.01.T01 | MTR_8g059275 | 32.979 | 188 | 111 | 6 | 55 | 232 | 1066 | 1248 | 2.18e-17 | 81.6 |
| MsG0180002385.01.T01 | MTR_8g059275 | 31.746 | 189 | 114 | 6 | 54 | 232 | 25 | 208 | 1.28e-15 | 76.3 |
| MsG0180002385.01.T01 | MTR_8g059275 | 36.316 | 190 | 106 | 6 | 55 | 234 | 1978 | 2162 | 1.75e-21 | 93.6 |
| MsG0180002385.01.T01 | MTR_8g059275 | 32.979 | 188 | 111 | 6 | 55 | 232 | 546 | 728 | 2.03e-17 | 81.6 |
| MsG0180002385.01.T01 | MTR_8g059275 | 32.979 | 188 | 111 | 6 | 55 | 232 | 1458 | 1640 | 2.03e-17 | 81.6 |
| MsG0180002385.01.T01 | MTR_8g059275 | 31.746 | 189 | 114 | 6 | 54 | 232 | 25 | 208 | 1.28e-15 | 76.3 |
| MsG0180002385.01.T01 | MTR_8g059275 | 28.384 | 229 | 148 | 7 | 14 | 232 | 898 | 1120 | 4.40e-15 | 74.7 |
| MsG0180002385.01.T01 | MTR_2g072020 | 34.091 | 176 | 107 | 4 | 55 | 222 | 991 | 1165 | 2.38e-21 | 93.2 |
| MsG0180002385.01.T01 | MTR_2g072110 | 31.868 | 182 | 113 | 4 | 55 | 228 | 1031 | 1209 | 9.25e-21 | 91.3 |
| MsG0180002385.01.T01 | MTR_2g072110 | 31.868 | 182 | 113 | 4 | 55 | 228 | 1031 | 1209 | 1.24e-20 | 90.9 |
| MsG0180002385.01.T01 | MTR_1g050230 | 36.747 | 166 | 88 | 5 | 55 | 205 | 251 | 414 | 4.02e-20 | 88.6 |
| MsG0180002385.01.T01 | MTR_0069s0080 | 34.409 | 186 | 111 | 5 | 55 | 232 | 539 | 721 | 8.26e-20 | 88.6 |
| MsG0180002385.01.T01 | MTR_0069s0080 | 33.166 | 199 | 116 | 7 | 51 | 239 | 13 | 204 | 6.42e-19 | 85.9 |
| MsG0180002385.01.T01 | MTR_0069s0080 | 31.746 | 189 | 114 | 6 | 54 | 232 | 1029 | 1212 | 1.20e-16 | 79.3 |
| MsG0180002385.01.T01 | MTR_2g072150 | 33.516 | 182 | 110 | 5 | 55 | 228 | 1023 | 1201 | 2.21e-19 | 87.4 |
| MsG0180002385.01.T01 | MTR_2g071960 | 32.203 | 177 | 109 | 5 | 60 | 228 | 878 | 1051 | 2.33e-19 | 87.0 |
| MsG0180002385.01.T01 | MTR_2g071980 | 32.203 | 177 | 109 | 5 | 60 | 228 | 988 | 1161 | 2.69e-19 | 87.0 |
| MsG0180002385.01.T01 | MTR_2g071850 | 32.203 | 177 | 109 | 5 | 60 | 228 | 1008 | 1181 | 2.82e-19 | 87.0 |
| MsG0180002385.01.T01 | MTR_2g072090 | 32.203 | 177 | 109 | 5 | 60 | 228 | 1008 | 1181 | 8.49e-19 | 85.5 |
| MsG0180002385.01.T01 | MTR_2g071870 | 32.203 | 177 | 109 | 5 | 60 | 228 | 1008 | 1181 | 8.50e-19 | 85.5 |
| MsG0180002385.01.T01 | MTR_8g059325 | 34.536 | 194 | 112 | 7 | 55 | 239 | 1 | 188 | 1.32e-18 | 81.3 |
| MsG0180002385.01.T01 | MTR_0006s0290 | 33.163 | 196 | 106 | 7 | 39 | 217 | 1 | 188 | 3.85e-18 | 82.8 |
| MsG0180002385.01.T01 | MTR_1g036880 | 32.487 | 197 | 116 | 7 | 53 | 239 | 36 | 225 | 8.90e-18 | 82.0 |
| MsG0180002385.01.T01 | MTR_1g036820 | 32.828 | 198 | 116 | 7 | 52 | 239 | 23 | 213 | 1.78e-17 | 81.6 |
| MsG0180002385.01.T01 | MTR_0460s0010 | 31.383 | 188 | 114 | 6 | 55 | 232 | 1 | 183 | 2.02e-17 | 81.3 |
| MsG0180002385.01.T01 | MTR_0460s0010 | 31.383 | 188 | 114 | 6 | 55 | 232 | 518 | 700 | 2.64e-17 | 80.9 |
| MsG0180002385.01.T01 | MTR_1g050395 | 33.871 | 186 | 115 | 3 | 55 | 232 | 226 | 411 | 2.66e-16 | 78.2 |
| MsG0180002385.01.T01 | MTR_0006s0250 | 31.915 | 188 | 117 | 5 | 53 | 232 | 12 | 196 | 4.33e-16 | 77.4 |
| MsG0180002385.01.T01 | MTR_8g059385 | 30.000 | 190 | 118 | 6 | 53 | 232 | 8 | 192 | 1.71e-15 | 75.1 |
| MsG0180002385.01.T01 | MTR_8g028520 | 31.579 | 171 | 108 | 2 | 69 | 232 | 1516 | 1684 | 2.14e-15 | 75.5 |
| MsG0180002385.01.T01 | MTR_8g028520 | 28.125 | 160 | 75 | 2 | 57 | 211 | 1034 | 1158 | 1.26e-12 | 67.4 |
| MsG0180002385.01.T01 | MTR_2g072080 | 26.842 | 190 | 105 | 5 | 73 | 232 | 950 | 1135 | 6.64e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 40 sgRNAs with CRISPR-Local
Find 99 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAATTGGCTGGAACTCTATT+TGG | 0.132513 | 1:-37801677 | MsG0180002385.01.T01:CDS |
| CAACTAAGTGTTGTAAATTT+AGG | 0.199268 | 1:-37800259 | MsG0180002385.01.T01:CDS |
| TGTGAAAAGTCAAATCTAAT+TGG | 0.254810 | 1:-37801693 | MsG0180002385.01.T01:CDS |
| ACATCTTCAATGTTTCAAAT+TGG | 0.268608 | 1:+37800704 | None:intergenic |
| TAGTTGATTAAACTCAAATA+TGG | 0.269606 | 1:+37800276 | None:intergenic |
| GGAACCACTTCGGACAATTC+TGG | 0.310264 | 1:-37801655 | MsG0180002385.01.T01:CDS |
| GAAAATCATACTGAAGGATA+TGG | 0.337563 | 1:-37800752 | MsG0180002385.01.T01:CDS |
| AATTGGCTGGAACTCTATTT+GGG | 0.374779 | 1:-37801676 | MsG0180002385.01.T01:CDS |
| TATTCACGGAAACGAAAATT+CGG | 0.389255 | 1:+37801777 | None:intergenic |
| TCTAATAAATGTACAATTGA+AGG | 0.407603 | 1:-37800437 | MsG0180002385.01.T01:CDS |
| TGAAAACTAAGAATTCCTTC+AGG | 0.410761 | 1:+37800463 | None:intergenic |
| TGGACAGCTTGAAGTCAATA+TGG | 0.416437 | 1:-37800732 | MsG0180002385.01.T01:CDS |
| TCTTCAATGTTTCAAATTGG+TGG | 0.439131 | 1:+37800707 | None:intergenic |
| ATTGAAGGGGTGTGCAAGCT+TGG | 0.439192 | 1:-37800422 | MsG0180002385.01.T01:CDS |
| CAACGAGTGGCTACGGAGAA+TGG | 0.442406 | 1:+37800388 | None:intergenic |
| AGTTGATTAAACTCAAATAT+GGG | 0.463014 | 1:+37800277 | None:intergenic |
| AAAAGTCAAATCTAATTGGC+TGG | 0.466289 | 1:-37801689 | MsG0180002385.01.T01:CDS |
| ATTCACGGAAACGAAAATTC+GGG | 0.471374 | 1:+37801778 | None:intergenic |
| TGCTCACATCTGAAGGAACT+TGG | 0.479511 | 1:-37800367 | MsG0180002385.01.T01:CDS |
| TGCAACAATTTCCTTCATGT+TGG | 0.484700 | 1:+37800330 | None:intergenic |
| ATTTGAAACATTGAAGATGT+TGG | 0.489790 | 1:-37800701 | MsG0180002385.01.T01:CDS |
| GGTTACAACACATTTGAAAG+AGG | 0.511350 | 1:-37800533 | MsG0180002385.01.T01:CDS |
| TATAGCCATCGTGATATTCA+CGG | 0.513517 | 1:+37801763 | None:intergenic |
| ATATAATAGTCTAGAGATGT+TGG | 0.516330 | 1:-37800623 | MsG0180002385.01.T01:CDS |
| CTAATAAATGTACAATTGAA+GGG | 0.516552 | 1:-37800436 | MsG0180002385.01.T01:CDS |
| AAAATATTGTTCCAACATGA+AGG | 0.518315 | 1:-37800341 | MsG0180002385.01.T01:CDS |
| ATGTGAGCAACGAGTGGCTA+CGG | 0.519078 | 1:+37800381 | None:intergenic |
| CACTCGTTGCTCACATCTGA+AGG | 0.540017 | 1:-37800374 | MsG0180002385.01.T01:CDS |
| ACTCTATTTGGGAACCACTT+CGG | 0.542629 | 1:-37801665 | MsG0180002385.01.T01:CDS |
| ATTAGAGAAAATCATACTGA+AGG | 0.563175 | 1:-37800758 | MsG0180002385.01.T01:CDS |
| CATGAAGGAAATTGTTGCAG+AGG | 0.592458 | 1:-37800326 | MsG0180002385.01.T01:CDS |
| TAATAAATGTACAATTGAAG+GGG | 0.606153 | 1:-37800435 | MsG0180002385.01.T01:CDS |
| GAACAATTGTAAGAAAATTG+TGG | 0.608664 | 1:-37800674 | MsG0180002385.01.T01:CDS |
| AAATGAAAATAACAGCGAAG+AGG | 0.621408 | 1:-37800554 | MsG0180002385.01.T01:CDS |
| ACTTCGGACAATTCTGGTCG+CGG | 0.630888 | 1:-37801649 | MsG0180002385.01.T01:intron |
| GCGACCAGAATTGTCCGAAG+TGG | 0.651180 | 1:+37801651 | None:intergenic |
| CAATTGTAAGAAAATTGTGG+TGG | 0.654075 | 1:-37800671 | MsG0180002385.01.T01:CDS |
| CGTTTCCGTGAATATCACGA+TGG | 0.654485 | 1:-37801768 | MsG0180002385.01.T01:CDS |
| CTTCAGATGTGAGCAACGAG+TGG | 0.656593 | 1:+37800375 | None:intergenic |
| ATATGGAGTGGAGATCCTGA+AGG | 0.666425 | 1:-37800478 | MsG0180002385.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAAAAAACACAAAA+GGG | - | Chr1:37800736-37800755 | MsG0180002385.01.T01:CDS | 10.0% |
| !! | AAAAATATACAACATAAATA+GGG | - | Chr1:37800649-37800668 | MsG0180002385.01.T01:CDS | 10.0% |
| !! | AATATACATTGAAATTTAAA+AGG | - | Chr1:37800695-37800714 | MsG0180002385.01.T01:CDS | 10.0% |
| !! | ATATACATTGAAATTTAAAA+GGG | - | Chr1:37800696-37800715 | MsG0180002385.01.T01:CDS | 10.0% |
| !!! | ATGTTAATTTGTTTTTATAT+AGG | - | Chr1:37800888-37800907 | MsG0180002385.01.T01:intron | 10.0% |
| !! | CAAAAAAAAAAAAACACAAA+AGG | - | Chr1:37800735-37800754 | MsG0180002385.01.T01:CDS | 15.0% |
| !! | GAAAAATATACAACATAAAT+AGG | - | Chr1:37800648-37800667 | MsG0180002385.01.T01:CDS | 15.0% |
| !! | TATACATTGAAATTTAAAAG+GGG | - | Chr1:37800697-37800716 | MsG0180002385.01.T01:CDS | 15.0% |
| !!! | ATTGGAAGTTTAAAATTAAT+TGG | + | Chr1:37801189-37801208 | None:intergenic | 15.0% |
| !! | AGTTGATTAAACTCAAATAT+GGG | + | Chr1:37801752-37801771 | None:intergenic | 20.0% |
| !! | CTAATAAATGTACAATTGAA+GGG | - | Chr1:37801590-37801609 | MsG0180002385.01.T01:intron | 20.0% |
| !! | GTCTAAATGAAGATTATATT+AGG | - | Chr1:37800418-37800437 | MsG0180002385.01.T01:CDS | 20.0% |
| !! | TAAGCAAAATTTAACTACTT+TGG | + | Chr1:37800464-37800483 | None:intergenic | 20.0% |
| !! | TAATAAATGTACAATTGAAG+GGG | - | Chr1:37801591-37801610 | MsG0180002385.01.T01:intron | 20.0% |
| !! | TAGTTGATTAAACTCAAATA+TGG | + | Chr1:37801753-37801772 | None:intergenic | 20.0% |
| !! | TCTAAATTTGAATCAAGTTT+GGG | - | Chr1:37800950-37800969 | MsG0180002385.01.T01:intron | 20.0% |
| !! | TCTAATAAATGTACAATTGA+AGG | - | Chr1:37801589-37801608 | MsG0180002385.01.T01:intron | 20.0% |
| !! | TTCTAAATTTGAATCAAGTT+TGG | - | Chr1:37800949-37800968 | MsG0180002385.01.T01:intron | 20.0% |
| !!! | ACAAAAGGGTATTATAATTA+GGG | - | Chr1:37800750-37800769 | MsG0180002385.01.T01:CDS | 20.0% |
| ! | AAAATATTGTTCCAACATGA+AGG | - | Chr1:37801685-37801704 | MsG0180002385.01.T01:CDS | 25.0% |
| ! | ACAATCAACTTTCTTAATTC+CGG | + | Chr1:37800530-37800549 | None:intergenic | 25.0% |
| ! | ACATCTTCAATGTTTCAAAT+TGG | + | Chr1:37801325-37801344 | None:intergenic | 25.0% |
| ! | ATATAATAGTCTAGAGATGT+TGG | - | Chr1:37801403-37801422 | MsG0180002385.01.T01:intron | 25.0% |
| ! | ATTAGAGAAAATCATACTGA+AGG | - | Chr1:37801268-37801287 | MsG0180002385.01.T01:intron | 25.0% |
| ! | CACTAATTCTCTTTCATAAA+GGG | + | Chr1:37800595-37800614 | None:intergenic | 25.0% |
| ! | GAACAATTGTAAGAAAATTG+TGG | - | Chr1:37801352-37801371 | MsG0180002385.01.T01:intron | 25.0% |
| ! | TAGGACAATTACTTATTTCA+AGG | + | Chr1:37801089-37801108 | None:intergenic | 25.0% |
| ! | TGTGAAAAGTCAAATCTAAT+TGG | - | Chr1:37800333-37800352 | MsG0180002385.01.T01:CDS | 25.0% |
| !! | AGGCATGGTAATTTTATTAT+TGG | - | Chr1:37801157-37801176 | MsG0180002385.01.T01:intron | 25.0% |
| !! | ATTTGAAACATTGAAGATGT+TGG | - | Chr1:37801325-37801344 | MsG0180002385.01.T01:intron | 25.0% |
| !! | CAACTAAGTGTTGTAAATTT+AGG | - | Chr1:37801767-37801786 | MsG0180002385.01.T01:CDS | 25.0% |
| !! | GCATGGTAATTTTATTATTG+GGG | - | Chr1:37801159-37801178 | MsG0180002385.01.T01:intron | 25.0% |
| !! | GGCATGGTAATTTTATTATT+GGG | - | Chr1:37801158-37801177 | MsG0180002385.01.T01:intron | 25.0% |
| !! | TGACTTTTCACATAAAACAT+GGG | + | Chr1:37800325-37800344 | None:intergenic | 25.0% |
| !! | TTGACTTTTCACATAAAACA+TGG | + | Chr1:37800326-37800345 | None:intergenic | 25.0% |
| !!! | AATTAGTGGTCGTTTTTATT+GGG | - | Chr1:37800607-37800626 | MsG0180002385.01.T01:CDS | 25.0% |
| !!! | CACAAAAGGGTATTATAATT+AGG | - | Chr1:37800749-37800768 | MsG0180002385.01.T01:CDS | 25.0% |
| !!! | TTGAAAGAGGTTATTTTAGA+TGG | - | Chr1:37801506-37801525 | MsG0180002385.01.T01:intron | 25.0% |
| AAAAGTCAAATCTAATTGGC+TGG | - | Chr1:37800337-37800356 | MsG0180002385.01.T01:CDS | 30.0% | |
| AAATGAAAATAACAGCGAAG+AGG | - | Chr1:37801472-37801491 | MsG0180002385.01.T01:intron | 30.0% | |
| AAGTAATTGTCCTATGATGT+AGG | - | Chr1:37801095-37801114 | MsG0180002385.01.T01:intron | 30.0% | |
| ATTATATGTGTGTGTGTGTA+TGG | - | Chr1:37800833-37800852 | MsG0180002385.01.T01:intron | 30.0% | |
| CAATTGTAAGAAAATTGTGG+TGG | - | Chr1:37801355-37801374 | MsG0180002385.01.T01:intron | 30.0% | |
| CCACTAATTCTCTTTCATAA+AGG | + | Chr1:37800596-37800615 | None:intergenic | 30.0% | |
| CCTTTATGAAAGAGAATTAG+TGG | - | Chr1:37800593-37800612 | MsG0180002385.01.T01:CDS | 30.0% | |
| GAAAATCATACTGAAGGATA+TGG | - | Chr1:37801274-37801293 | MsG0180002385.01.T01:intron | 30.0% | |
| GTATTTATTCTCGTCTACTT+TGG | - | Chr1:37801037-37801056 | MsG0180002385.01.T01:intron | 30.0% | |
| GTTGTTGTGTATGAATTCAT+AGG | - | Chr1:37801232-37801251 | MsG0180002385.01.T01:intron | 30.0% | |
| TAGAAGTGAGCTCAATTTAA+GGG | + | Chr1:37800934-37800953 | None:intergenic | 30.0% | |
| TATTCACGGAAACGAAAATT+CGG | + | Chr1:37800252-37800271 | None:intergenic | 30.0% | |
| TCTTCAATGTTTCAAATTGG+TGG | + | Chr1:37801322-37801341 | None:intergenic | 30.0% | |
| TCTTTCATAAAGGGTAAACA+TGG | + | Chr1:37800586-37800605 | None:intergenic | 30.0% | |
| TGAAAACTAAGAATTCCTTC+AGG | + | Chr1:37801566-37801585 | None:intergenic | 30.0% | |
| TTAGAAGTGAGCTCAATTTA+AGG | + | Chr1:37800935-37800954 | None:intergenic | 30.0% | |
| ! | TACCTCCTTCCTTATTTATA+GGG | - | Chr1:37800487-37800506 | MsG0180002385.01.T01:CDS | 30.0% |
| !! | AAGGGTATTATAATTAGGGA+TGG | - | Chr1:37800754-37800773 | MsG0180002385.01.T01:CDS | 30.0% |
| !!! | ATTCCGGTGTTTTTTGTAAA+AGG | + | Chr1:37800514-37800533 | None:intergenic | 30.0% |
| !!! | GAATTAGTGGTCGTTTTTAT+TGG | - | Chr1:37800606-37800625 | MsG0180002385.01.T01:CDS | 30.0% |
| !!! | TTCCGGTGTTTTTTGTAAAA+GGG | + | Chr1:37800513-37800532 | None:intergenic | 30.0% |
| AAGCTGAAAAAGATATGGAG+TGG | - | Chr1:37801536-37801555 | MsG0180002385.01.T01:intron | 35.0% | |
| AATTGGCTGGAACTCTATTT+GGG | - | Chr1:37800350-37800369 | MsG0180002385.01.T01:CDS | 35.0% | |
| ATTCACGGAAACGAAAATTC+GGG | + | Chr1:37800251-37800270 | None:intergenic | 35.0% | |
| GCTATTATCTCCTACATCAT+AGG | + | Chr1:37801108-37801127 | None:intergenic | 35.0% | |
| GGTATTATAATTAGGGATGG+AGG | - | Chr1:37800757-37800776 | MsG0180002385.01.T01:CDS | 35.0% | |
| GGTTACAACACATTTGAAAG+AGG | - | Chr1:37801493-37801512 | MsG0180002385.01.T01:intron | 35.0% | |
| GTAAAAGGGCCCTATAAATA+AGG | + | Chr1:37800499-37800518 | None:intergenic | 35.0% | |
| TAATTGGCTGGAACTCTATT+TGG | - | Chr1:37800349-37800368 | MsG0180002385.01.T01:CDS | 35.0% | |
| TATAGCCATCGTGATATTCA+CGG | + | Chr1:37800266-37800285 | None:intergenic | 35.0% | |
| TATAGGTTGCATTTCTCAGT+TGG | - | Chr1:37800905-37800924 | MsG0180002385.01.T01:intron | 35.0% | |
| TGCAACAATTTCCTTCATGT+TGG | + | Chr1:37801699-37801718 | None:intergenic | 35.0% | |
| TGTCGAAGCTGAAAAAGATA+TGG | - | Chr1:37801531-37801550 | MsG0180002385.01.T01:intron | 35.0% | |
| ! | ATATGTGTTTTGCACTGAAG+AGG | + | Chr1:37801387-37801406 | None:intergenic | 35.0% |
| ! | CTACCTCCTTCCTTATTTAT+AGG | - | Chr1:37800486-37800505 | MsG0180002385.01.T01:CDS | 35.0% |
| ! | CTTTTCACATAAAACATGGG+TGG | + | Chr1:37800322-37800341 | None:intergenic | 35.0% |
| ! | TATGTGTTTTGCACTGAAGA+GGG | + | Chr1:37801386-37801405 | None:intergenic | 35.0% |
| AAGGGCCCTATAAATAAGGA+AGG | + | Chr1:37800495-37800514 | None:intergenic | 40.0% | |
| ACTCTATTTGGGAACCACTT+CGG | - | Chr1:37800361-37800380 | MsG0180002385.01.T01:CDS | 40.0% | |
| AGCTTGATTGTGGATAGTTG+AGG | - | Chr1:37801005-37801024 | MsG0180002385.01.T01:intron | 40.0% | |
| CATGAAGGAAATTGTTGCAG+AGG | - | Chr1:37801700-37801719 | MsG0180002385.01.T01:CDS | 40.0% | |
| TGGACAGCTTGAAGTCAATA+TGG | - | Chr1:37801294-37801313 | MsG0180002385.01.T01:intron | 40.0% | |
| TTGATTGTGGATAGTTGAGG+TGG | - | Chr1:37801008-37801027 | MsG0180002385.01.T01:intron | 40.0% | |
| ! | GGCCCTTTTACAAAAAACAC+CGG | - | Chr1:37800508-37800527 | MsG0180002385.01.T01:CDS | 40.0% |
| !! | CAACTTGACTAGCTTGATTG+TGG | - | Chr1:37800995-37801014 | MsG0180002385.01.T01:intron | 40.0% |
| ATATGGAGTGGAGATCCTGA+AGG | - | Chr1:37801548-37801567 | MsG0180002385.01.T01:intron | 45.0% | |
| CGTTTCCGTGAATATCACGA+TGG | - | Chr1:37800258-37800277 | MsG0180002385.01.T01:CDS | 45.0% | |
| GGCCCTATAAATAAGGAAGG+AGG | + | Chr1:37800492-37800511 | None:intergenic | 45.0% | |
| TGCTCACATCTGAAGGAACT+TGG | - | Chr1:37801659-37801678 | MsG0180002385.01.T01:CDS | 45.0% | |
| !! | GTATAAATAATTATTTAAAT+TGG | + | Chr1:37801207-37801226 | None:intergenic | 5.0% |
| ACTTCGGACAATTCTGGTCG+CGG | - | Chr1:37800377-37800396 | MsG0180002385.01.T01:CDS | 50.0% | |
| ATGTGAGCAACGAGTGGCTA+CGG | + | Chr1:37801648-37801667 | None:intergenic | 50.0% | |
| CACTCGTTGCTCACATCTGA+AGG | - | Chr1:37801652-37801671 | MsG0180002385.01.T01:CDS | 50.0% | |
| CAGAAACCATGCGCTGAAAC+AGG | - | Chr1:37801137-37801156 | MsG0180002385.01.T01:intron | 50.0% | |
| CTTCAGATGTGAGCAACGAG+TGG | + | Chr1:37801654-37801673 | None:intergenic | 50.0% | |
| GGAACCACTTCGGACAATTC+TGG | - | Chr1:37800371-37800390 | MsG0180002385.01.T01:CDS | 50.0% | |
| ! | ATTGAAGGGGTGTGCAAGCT+TGG | - | Chr1:37801604-37801623 | MsG0180002385.01.T01:intron | 50.0% |
| ACCATGCCTGTTTCAGCGCA+TGG | + | Chr1:37801146-37801165 | None:intergenic | 55.0% | |
| ACCATGCGCTGAAACAGGCA+TGG | - | Chr1:37801142-37801161 | MsG0180002385.01.T01:intron | 55.0% | |
| CAACGAGTGGCTACGGAGAA+TGG | + | Chr1:37801641-37801660 | None:intergenic | 55.0% | |
| GCGACCAGAATTGTCCGAAG+TGG | + | Chr1:37800378-37800397 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 37800237 | 37801811 | 37800237 | ID=MsG0180002385.01;Name=MsG0180002385.01 |
| Chr1 | mRNA | 37800237 | 37801811 | 37800237 | ID=MsG0180002385.01.T01;Parent=MsG0180002385.01;Name=MsG0180002385.01.T01;_AED=0.43;_eAED=0.43;_QI=0|0|0|0.5|1|1|2|0|239 |
| Chr1 | exon | 37801650 | 37801811 | 37801650 | ID=MsG0180002385.01.T01:exon:13277;Parent=MsG0180002385.01.T01 |
| Chr1 | exon | 37800237 | 37800794 | 37800237 | ID=MsG0180002385.01.T01:exon:13278;Parent=MsG0180002385.01.T01 |
| Chr1 | CDS | 37801650 | 37801811 | 37801650 | ID=MsG0180002385.01.T01:cds;Parent=MsG0180002385.01.T01 |
| Chr1 | CDS | 37800237 | 37800794 | 37800237 | ID=MsG0180002385.01.T01:cds;Parent=MsG0180002385.01.T01 |
| Gene Sequence |
| Protein sequence |
