Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002499.01.T01 | XP_013467414.1 | 95.42 | 262 | 4 | 1 | 2 | 263 | 3 | 256 | 0 | 510 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002499.01.T01 | O22197 | 44.512 | 328 | 116 | 9 | 1 | 263 | 1 | 327 | 5.47E-80 | 247 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002499.01.T01 | B7FGT7 | 95.420 | 262 | 4 | 1 | 2 | 263 | 3 | 256 | 0.0 | 510 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0080048848.01 | MsG0180002499.01 | PPI |
| MsG0480023815.01 | MsG0180002499.01 | PPI |
| MsG0380015056.01 | MsG0180002499.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002499.01.T01 | MTR_1g051105 | 95.420 | 262 | 4 | 1 | 2 | 263 | 3 | 256 | 0.0 | 510 |
| MsG0180002499.01.T01 | MTR_5g088720 | 40.719 | 334 | 118 | 8 | 1 | 263 | 5 | 329 | 7.03e-71 | 221 |
| MsG0180002499.01.T01 | MTR_5g088720 | 40.964 | 332 | 116 | 8 | 3 | 263 | 2 | 324 | 8.68e-71 | 221 |
| MsG0180002499.01.T01 | MTR_5g088720 | 40.719 | 334 | 118 | 8 | 1 | 263 | 46 | 370 | 1.20e-70 | 221 |
| MsG0180002499.01.T01 | MTR_0067s0100 | 40.339 | 295 | 100 | 9 | 1 | 235 | 1 | 279 | 1.37e-56 | 182 |
| MsG0180002499.01.T01 | MTR_3g072630 | 58.416 | 101 | 42 | 0 | 114 | 214 | 169 | 269 | 3.33e-40 | 142 |
| MsG0180002499.01.T01 | MTR_3g072630 | 58.416 | 101 | 42 | 0 | 114 | 214 | 169 | 269 | 4.91e-40 | 141 |
| MsG0180002499.01.T01 | MTR_3g068005 | 33.878 | 245 | 130 | 7 | 1 | 214 | 1 | 244 | 2.73e-36 | 133 |
| MsG0180002499.01.T01 | MTR_6g079660 | 47.015 | 134 | 56 | 4 | 81 | 211 | 159 | 280 | 4.39e-35 | 129 |
| MsG0180002499.01.T01 | MTR_4g064570 | 33.333 | 225 | 121 | 9 | 7 | 211 | 11 | 226 | 2.31e-30 | 117 |
| MsG0180002499.01.T01 | MTR_5g080350 | 30.337 | 267 | 143 | 9 | 7 | 239 | 3 | 260 | 3.13e-29 | 114 |
| MsG0180002499.01.T01 | MTR_1g114320 | 46.789 | 109 | 55 | 2 | 114 | 219 | 139 | 247 | 3.89e-29 | 112 |
| MsG0180002499.01.T01 | MTR_3g083480 | 50.495 | 101 | 43 | 2 | 112 | 210 | 186 | 281 | 8.32e-28 | 109 |
| MsG0180002499.01.T01 | MTR_2g007340 | 48.624 | 109 | 55 | 1 | 116 | 223 | 150 | 258 | 2.55e-26 | 105 |
| MsG0180002499.01.T01 | MTR_1g114620 | 45.631 | 103 | 51 | 2 | 112 | 211 | 209 | 309 | 2.69e-24 | 99.8 |
| MsG0180002499.01.T01 | MTR_7g074170 | 46.067 | 89 | 45 | 2 | 126 | 211 | 302 | 390 | 8.11e-22 | 94.7 |
| MsG0180002499.01.T01 | MTR_5g041310 | 37.624 | 101 | 59 | 2 | 112 | 211 | 63 | 160 | 2.82e-18 | 81.3 |
| MsG0180002499.01.T01 | MTR_5g041330 | 38.462 | 104 | 53 | 2 | 116 | 211 | 50 | 150 | 3.82e-18 | 80.9 |
| MsG0180002499.01.T01 | MTR_6g032960 | 37.615 | 109 | 59 | 3 | 107 | 211 | 178 | 281 | 2.83e-17 | 80.5 |
| MsG0180002499.01.T01 | MTR_4g110100 | 32.759 | 116 | 72 | 3 | 112 | 221 | 303 | 418 | 2.49e-16 | 78.6 |
| MsG0180002499.01.T01 | MTR_1g014710 | 30.328 | 122 | 73 | 3 | 101 | 210 | 156 | 277 | 5.07e-16 | 76.6 |
| MsG0180002499.01.T01 | MTR_5g058530 | 48.649 | 74 | 36 | 2 | 152 | 224 | 209 | 281 | 1.20e-15 | 75.9 |
| MsG0180002499.01.T01 | MTR_6g016350 | 38.614 | 101 | 53 | 3 | 127 | 218 | 269 | 369 | 1.03e-14 | 73.6 |
| MsG0180002499.01.T01 | MTR_3g088080 | 37.333 | 75 | 45 | 1 | 141 | 215 | 15 | 87 | 1.46e-14 | 67.8 |
| MsG0180002499.01.T01 | MTR_5g022920 | 38.750 | 80 | 45 | 2 | 135 | 210 | 155 | 234 | 1.25e-12 | 65.9 |
| MsG0180002499.01.T01 | MTR_8g074900 | 35.577 | 104 | 63 | 2 | 108 | 210 | 125 | 225 | 3.34e-12 | 64.7 |
| MsG0180002499.01.T01 | MTR_3g031000 | 39.437 | 71 | 35 | 2 | 141 | 211 | 279 | 341 | 3.14e-11 | 63.2 |
| MsG0180002499.01.T01 | MTR_3g025510 | 44.000 | 50 | 28 | 0 | 166 | 215 | 105 | 154 | 5.88e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002499.01.T01 | AT2G40830 | 44.512 | 328 | 116 | 9 | 1 | 263 | 1 | 327 | 5.58e-81 | 247 |
| MsG0180002499.01.T01 | AT2G40830 | 44.512 | 328 | 116 | 9 | 1 | 263 | 1 | 327 | 5.58e-81 | 247 |
| MsG0180002499.01.T01 | AT2G40830 | 44.512 | 328 | 116 | 9 | 1 | 263 | 1 | 327 | 5.58e-81 | 247 |
| MsG0180002499.01.T01 | AT2G40830 | 44.512 | 328 | 116 | 9 | 1 | 263 | 1 | 327 | 5.58e-81 | 247 |
| MsG0180002499.01.T01 | AT3G56580 | 46.038 | 265 | 105 | 10 | 1 | 227 | 1 | 265 | 8.15e-70 | 218 |
| MsG0180002499.01.T01 | AT3G56580 | 46.038 | 265 | 105 | 10 | 1 | 227 | 1 | 265 | 8.15e-70 | 218 |
| MsG0180002499.01.T01 | AT3G56580 | 46.038 | 265 | 105 | 10 | 1 | 227 | 1 | 265 | 8.15e-70 | 218 |
| MsG0180002499.01.T01 | AT3G10815 | 38.235 | 238 | 93 | 6 | 6 | 239 | 8 | 195 | 1.02e-47 | 157 |
| MsG0180002499.01.T01 | AT3G19950 | 54.545 | 110 | 47 | 2 | 113 | 219 | 160 | 269 | 3.99e-36 | 131 |
| MsG0180002499.01.T01 | AT3G19950 | 54.545 | 110 | 47 | 2 | 113 | 219 | 218 | 327 | 4.33e-36 | 132 |
| MsG0180002499.01.T01 | AT3G19950 | 54.545 | 110 | 47 | 2 | 113 | 219 | 218 | 327 | 4.33e-36 | 132 |
| MsG0180002499.01.T01 | AT5G59550 | 33.898 | 236 | 133 | 5 | 7 | 219 | 17 | 252 | 3.83e-32 | 122 |
| MsG0180002499.01.T01 | AT5G59550 | 33.898 | 236 | 133 | 5 | 7 | 219 | 122 | 357 | 6.38e-32 | 123 |
| MsG0180002499.01.T01 | AT1G60360 | 40.000 | 170 | 77 | 5 | 96 | 243 | 155 | 321 | 1.36e-31 | 119 |
| MsG0180002499.01.T01 | AT3G46620 | 31.381 | 239 | 138 | 5 | 7 | 219 | 30 | 268 | 3.12e-31 | 119 |
| MsG0180002499.01.T01 | AT3G13430 | 49.533 | 107 | 47 | 2 | 108 | 211 | 168 | 270 | 4.78e-30 | 115 |
| MsG0180002499.01.T01 | AT3G13430 | 49.533 | 107 | 47 | 2 | 108 | 211 | 168 | 270 | 4.78e-30 | 115 |
| MsG0180002499.01.T01 | AT3G13430 | 49.533 | 107 | 47 | 2 | 108 | 211 | 168 | 270 | 4.78e-30 | 115 |
| MsG0180002499.01.T01 | AT3G13430 | 49.533 | 107 | 47 | 2 | 108 | 211 | 168 | 270 | 4.78e-30 | 115 |
| MsG0180002499.01.T01 | AT1G55530 | 46.296 | 108 | 51 | 2 | 111 | 216 | 138 | 240 | 4.17e-29 | 112 |
| MsG0180002499.01.T01 | AT1G55530 | 43.966 | 116 | 58 | 2 | 103 | 216 | 163 | 273 | 3.82e-28 | 110 |
| MsG0180002499.01.T01 | AT1G55530 | 43.966 | 116 | 58 | 2 | 103 | 216 | 163 | 273 | 3.82e-28 | 110 |
| MsG0180002499.01.T01 | AT5G56340 | 52.041 | 98 | 38 | 3 | 117 | 211 | 213 | 304 | 5.59e-27 | 108 |
| MsG0180002499.01.T01 | AT4G26400 | 46.729 | 107 | 50 | 2 | 117 | 221 | 195 | 296 | 7.50e-26 | 104 |
| MsG0180002499.01.T01 | AT4G26400 | 46.729 | 107 | 50 | 2 | 117 | 221 | 195 | 296 | 7.50e-26 | 104 |
| MsG0180002499.01.T01 | AT2G39720 | 30.204 | 245 | 132 | 8 | 7 | 213 | 5 | 248 | 1.27e-25 | 104 |
| MsG0180002499.01.T01 | AT2G39720 | 30.204 | 245 | 132 | 8 | 7 | 213 | 5 | 248 | 1.27e-25 | 104 |
| MsG0180002499.01.T01 | AT5G01980 | 44.554 | 101 | 54 | 1 | 113 | 211 | 295 | 395 | 4.47e-23 | 98.2 |
| MsG0180002499.01.T01 | AT1G26800 | 39.623 | 106 | 57 | 2 | 123 | 225 | 74 | 175 | 2.06e-20 | 86.7 |
| MsG0180002499.01.T01 | AT1G68180 | 39.450 | 109 | 64 | 1 | 138 | 246 | 110 | 216 | 3.37e-20 | 87.0 |
| MsG0180002499.01.T01 | AT5G15820 | 46.053 | 76 | 40 | 1 | 137 | 211 | 261 | 336 | 1.29e-19 | 87.4 |
| MsG0180002499.01.T01 | AT3G60080 | 52.632 | 76 | 28 | 2 | 147 | 214 | 142 | 217 | 5.55e-19 | 84.7 |
| MsG0180002499.01.T01 | AT3G02340 | 46.053 | 76 | 40 | 1 | 137 | 211 | 305 | 380 | 6.18e-19 | 85.9 |
| MsG0180002499.01.T01 | AT3G02340 | 46.053 | 76 | 40 | 1 | 137 | 211 | 305 | 380 | 6.18e-19 | 85.9 |
| MsG0180002499.01.T01 | AT5G08139 | 34.109 | 129 | 76 | 3 | 97 | 216 | 231 | 359 | 1.39e-17 | 81.6 |
| MsG0180002499.01.T01 | AT5G20910 | 32.520 | 123 | 67 | 4 | 108 | 219 | 166 | 283 | 1.64e-17 | 80.9 |
| MsG0180002499.01.T01 | AT2G44330 | 52.941 | 51 | 24 | 0 | 166 | 216 | 96 | 146 | 1.41e-16 | 75.9 |
| MsG0180002499.01.T01 | AT5G64920 | 40.000 | 105 | 56 | 2 | 113 | 210 | 197 | 301 | 2.47e-16 | 77.8 |
| MsG0180002499.01.T01 | AT1G14200 | 38.824 | 85 | 52 | 0 | 127 | 211 | 70 | 154 | 4.20e-16 | 74.7 |
| MsG0180002499.01.T01 | AT5G60820 | 42.308 | 78 | 41 | 1 | 138 | 211 | 340 | 417 | 9.02e-16 | 76.6 |
| MsG0180002499.01.T01 | AT4G35840 | 36.170 | 94 | 54 | 3 | 123 | 210 | 143 | 236 | 6.24e-12 | 64.3 |
| MsG0180002499.01.T01 | AT3G60220 | 46.250 | 80 | 37 | 3 | 137 | 210 | 83 | 162 | 1.09e-11 | 64.3 |
| MsG0180002499.01.T01 | AT2G34990 | 33.557 | 149 | 86 | 5 | 114 | 256 | 46 | 187 | 2.70e-11 | 63.2 |
| MsG0180002499.01.T01 | AT2G34990 | 33.557 | 149 | 86 | 5 | 114 | 256 | 68 | 209 | 3.19e-11 | 63.2 |
| MsG0180002499.01.T01 | AT5G02750 | 50.000 | 46 | 23 | 0 | 166 | 211 | 214 | 259 | 3.57e-11 | 62.4 |
| MsG0180002499.01.T01 | AT3G63530 | 36.559 | 93 | 48 | 4 | 125 | 210 | 153 | 241 | 5.81e-11 | 61.6 |
| MsG0180002499.01.T01 | AT3G63530 | 36.559 | 93 | 48 | 4 | 125 | 210 | 153 | 241 | 5.81e-11 | 61.6 |
| MsG0180002499.01.T01 | AT2G44578 | 42.373 | 59 | 33 | 1 | 154 | 211 | 58 | 116 | 9.21e-11 | 59.3 |
Find 77 sgRNAs with CRISPR-Local
Find 87 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCCAGAATGCACAACATTC+TGG | 0.256129 | 1:-39268211 | MsG0180002499.01.T01:CDS |
| TAGAAAACCTGAATGGAATT+TGG | 0.277073 | 1:+39268707 | None:intergenic |
| ACTGCTCCTTTGTTAATCTA+TGG | 0.277633 | 1:-39268733 | MsG0180002499.01.T01:CDS |
| TTGCATGAGGCCGGTTCGAC+TGG | 0.318244 | 1:-39268929 | MsG0180002499.01.T01:CDS |
| ATGGTGGCCAAATTCCATTC+AGG | 0.345196 | 1:-39268714 | MsG0180002499.01.T01:CDS |
| TCAAATTTATCTTGGCATAC+TGG | 0.366225 | 1:+39268466 | None:intergenic |
| AGTAAACCTTGGAGCGTGTT+CGG | 0.366664 | 1:+39268753 | None:intergenic |
| GGCAATTCTTGACGGCAAAC+AGG | 0.368193 | 1:+39268355 | None:intergenic |
| GTAAACCTTGGAGCGTGTTC+GGG | 0.379716 | 1:+39268754 | None:intergenic |
| ACCCAGAATGTTGTGCATTC+TGG | 0.381915 | 1:+39268209 | None:intergenic |
| TGTTCAAGATCTCAACGATA+TGG | 0.382175 | 1:-39268866 | MsG0180002499.01.T01:CDS |
| AGAAGTAATGACGGAGCAAC+TGG | 0.400761 | 1:-39268268 | MsG0180002499.01.T01:CDS |
| ACCCAAGCTCAAATTTATCT+TGG | 0.413766 | 1:+39268458 | None:intergenic |
| CTCCTTCTTCCACTCTGTTG+TGG | 0.420170 | 1:+39268334 | None:intergenic |
| AAAGGCATCAAATGGCAGAT+AGG | 0.423444 | 1:-39268801 | MsG0180002499.01.T01:CDS |
| CCTATTGTGACACCAGTTCC+CGG | 0.428992 | 1:+39268649 | None:intergenic |
| TTTAATGGGACTCCGGGAAC+TGG | 0.436909 | 1:-39268661 | MsG0180002499.01.T01:CDS |
| CCGGGAACTGGTGTCACAAT+AGG | 0.439696 | 1:-39268649 | MsG0180002499.01.T01:CDS |
| GCCAAGATAAATTTGAGCTT+GGG | 0.443564 | 1:-39268459 | MsG0180002499.01.T01:CDS |
| CTCGACGATATCTTCACAAT+AGG | 0.447990 | 1:+39268514 | None:intergenic |
| TGCCAAGATAAATTTGAGCT+TGG | 0.448646 | 1:-39268460 | MsG0180002499.01.T01:CDS |
| ACCGTTTGCTCTAGTTGTGA+TGG | 0.452363 | 1:-39268895 | MsG0180002499.01.T01:CDS |
| GCCAGAATGCACAACATTCT+GGG | 0.459246 | 1:-39268210 | MsG0180002499.01.T01:CDS |
| CCGTTTGCTCTAGTTGTGAT+GGG | 0.467679 | 1:-39268894 | MsG0180002499.01.T01:CDS |
| AGAGGGAGGATGTCAAGTAC+TGG | 0.468477 | 1:-39268973 | None:intergenic |
| TGTCTCCGTCCCAGTCGAAC+CGG | 0.468565 | 1:+39268919 | None:intergenic |
| TCTGTTGTGGCAATTCTTGA+CGG | 0.472382 | 1:+39268347 | None:intergenic |
| CAAGTACTGGGGATACTCAT+TGG | 0.473480 | 1:-39268960 | MsG0180002499.01.T01:CDS |
| TGTCCACTCCATAGCTAGAA+TGG | 0.504718 | 1:+39268842 | None:intergenic |
| GCAGCATAAAGTCACTCAAA+TGG | 0.515787 | 1:+39268184 | None:intergenic |
| TTCTTGACGGCAAACAGGGC+AGG | 0.519855 | 1:+39268360 | None:intergenic |
| CCCATCACAACTAGAGCAAA+CGG | 0.522098 | 1:+39268894 | None:intergenic |
| CAATTCCCGAACACGCTCCA+AGG | 0.522110 | 1:-39268759 | MsG0180002499.01.T01:CDS |
| ACTCGGACAAAGGCATCAAA+TGG | 0.524038 | 1:-39268809 | MsG0180002499.01.T01:CDS |
| AATGGCAGATAGGGAACAAT+CGG | 0.524483 | 1:-39268791 | MsG0180002499.01.T01:CDS |
| AAGGCATCAAATGGCAGATA+GGG | 0.525527 | 1:-39268800 | MsG0180002499.01.T01:CDS |
| AACAGCTTTCCGCTAATGAT+CGG | 0.525841 | 1:-39268579 | MsG0180002499.01.T01:CDS |
| GTGTTATATTTGCATGAGGC+CGG | 0.527306 | 1:-39268938 | MsG0180002499.01.T01:CDS |
| TCTATAGATGACCGTGATGC+AGG | 0.536028 | 1:+39268544 | None:intergenic |
| GATATGGTCCATTCTAGCTA+TGG | 0.536606 | 1:-39268850 | MsG0180002499.01.T01:CDS |
| AACAAAGGAGCAGTAAACCT+TGG | 0.538084 | 1:+39268742 | None:intergenic |
| GCTCCTTTGTTAATCTATGG+TGG | 0.543536 | 1:-39268730 | MsG0180002499.01.T01:CDS |
| AGATGACCGTGATGCAGGTG+GGG | 0.544084 | 1:+39268549 | None:intergenic |
| CAGTTCTTCAACTCCAGGAC+CGG | 0.544221 | 1:+39268606 | None:intergenic |
| AGATAGGGAACAATCGGTCT+CGG | 0.544382 | 1:-39268785 | MsG0180002499.01.T01:CDS |
| AGATTGTATTGTACCATGGT+TGG | 0.544902 | 1:-39268395 | MsG0180002499.01.T01:CDS |
| CCTTGTCTAGAAAACCTGAA+TGG | 0.546308 | 1:+39268700 | None:intergenic |
| TGCATGAGGCCGGTTCGACT+GGG | 0.546824 | 1:-39268928 | MsG0180002499.01.T01:CDS |
| GAGGGAGGATGTCAAGTACT+GGG | 0.553091 | 1:-39268972 | None:intergenic |
| GAGTGAAACATATGTTTACA+TGG | 0.556582 | 1:+39268418 | None:intergenic |
| TGCCACAACAGAGTGGAAGA+AGG | 0.559947 | 1:-39268336 | MsG0180002499.01.T01:CDS |
| GCAATTCTTGACGGCAAACA+GGG | 0.564036 | 1:+39268356 | None:intergenic |
| CGTTTGCTCTAGTTGTGATG+GGG | 0.564195 | 1:-39268893 | MsG0180002499.01.T01:CDS |
| TCTAACAGTTCTTCAACTCC+AGG | 0.572791 | 1:+39268601 | None:intergenic |
| TAGATGACCGTGATGCAGGT+GGG | 0.577515 | 1:+39268548 | None:intergenic |
| GATGACCGTGATGCAGGTGG+GGG | 0.577553 | 1:+39268550 | None:intergenic |
| GGGACCTCTCCGATCATTAG+CGG | 0.584101 | 1:+39268570 | None:intergenic |
| ACGTTGAAGAACTCGGACAA+AGG | 0.588670 | 1:-39268819 | MsG0180002499.01.T01:CDS |
| GTGGACAACGTTGAAGAACT+CGG | 0.592915 | 1:-39268826 | MsG0180002499.01.T01:CDS |
| ATTGGTGTTATATTTGCATG+AGG | 0.593830 | 1:-39268942 | MsG0180002499.01.T01:CDS |
| ACTCAGATTGTATTGTACCA+TGG | 0.607235 | 1:-39268399 | MsG0180002499.01.T01:CDS |
| TGAGGCCGGTTCGACTGGGA+CGG | 0.611969 | 1:-39268924 | MsG0180002499.01.T01:CDS |
| GTTTGCTCTAGTTGTGATGG+GGG | 0.614331 | 1:-39268892 | MsG0180002499.01.T01:CDS |
| TGGCCACCATAGATTAACAA+AGG | 0.615532 | 1:+39268727 | None:intergenic |
| TCTAATTCTAGAAGTAATGA+CGG | 0.617506 | 1:-39268277 | MsG0180002499.01.T01:CDS |
| GGTCCATTCTAGCTATGGAG+TGG | 0.622374 | 1:-39268845 | MsG0180002499.01.T01:CDS |
| ATAGATGACCGTGATGCAGG+TGG | 0.626471 | 1:+39268547 | None:intergenic |
| TGGTGTCACAATAGGTAACT+CGG | 0.639331 | 1:-39268641 | MsG0180002499.01.T01:CDS |
| TCTTGGCATACTGGACAATG+TGG | 0.643630 | 1:+39268475 | None:intergenic |
| GGAAGCAGCTCACTAACACC+AGG | 0.649959 | 1:-39268247 | MsG0180002499.01.T01:CDS |
| CAAGAATTGCCACAACAGAG+TGG | 0.664197 | 1:-39268343 | MsG0180002499.01.T01:CDS |
| GTGTTATGCTGAACCAACCA+TGG | 0.677661 | 1:+39268382 | None:intergenic |
| CTAGAATTAGAAGAGCCGAA+AGG | 0.706598 | 1:+39268289 | None:intergenic |
| AGGGAGGATGTCAAGTACTG+GGG | 0.710941 | 1:-39268971 | None:intergenic |
| CTTTCCGCTAATGATCGGAG+AGG | 0.728161 | 1:-39268574 | MsG0180002499.01.T01:CDS |
| AGAATGCACAACATTCTGGG+TGG | 0.735908 | 1:-39268207 | MsG0180002499.01.T01:CDS |
| CTATTGTGAAGATATCGTCG+AGG | 0.739761 | 1:-39268513 | MsG0180002499.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTAATTCTAGAAGTAATGA+CGG | - | Chr1:39268882-39268901 | MsG0180002499.01.T01:CDS | 25.0% |
| GAGTGAAACATATGTTTACA+TGG | + | Chr1:39268744-39268763 | None:intergenic | 30.0% | |
| TAGAAAACCTGAATGGAATT+TGG | + | Chr1:39268455-39268474 | None:intergenic | 30.0% | |
| TCAAATTTATCTTGGCATAC+TGG | + | Chr1:39268696-39268715 | None:intergenic | 30.0% | |
| ! | AACTCGGTTAACTATTTTAC+CGG | - | Chr1:39268534-39268553 | MsG0180002499.01.T01:CDS | 30.0% |
| !! | ATTGGTGTTATATTTGCATG+AGG | - | Chr1:39268217-39268236 | MsG0180002499.01.T01:CDS | 30.0% |
| !! | GCATTGATGCTCTTTTTAAT+GGG | - | Chr1:39268484-39268503 | MsG0180002499.01.T01:CDS | 30.0% |
| AAAGGCCACAAAAACGAAAA+AGG | + | Chr1:39268855-39268874 | None:intergenic | 35.0% | |
| AAGGCCACAAAAACGAAAAA+GGG | + | Chr1:39268854-39268873 | None:intergenic | 35.0% | |
| ACCCAAGCTCAAATTTATCT+TGG | + | Chr1:39268704-39268723 | None:intergenic | 35.0% | |
| ACTCAGATTGTATTGTACCA+TGG | - | Chr1:39268760-39268779 | MsG0180002499.01.T01:CDS | 35.0% | |
| ACTGCTCCTTTGTTAATCTA+TGG | - | Chr1:39268426-39268445 | MsG0180002499.01.T01:CDS | 35.0% | |
| AGATTGTATTGTACCATGGT+TGG | - | Chr1:39268764-39268783 | MsG0180002499.01.T01:CDS | 35.0% | |
| GCCAAGATAAATTTGAGCTT+GGG | - | Chr1:39268700-39268719 | MsG0180002499.01.T01:CDS | 35.0% | |
| TGCCAAGATAAATTTGAGCT+TGG | - | Chr1:39268699-39268718 | MsG0180002499.01.T01:CDS | 35.0% | |
| TGTTCAAGATCTCAACGATA+TGG | - | Chr1:39268293-39268312 | MsG0180002499.01.T01:CDS | 35.0% | |
| !! | GAAACCCTTTTTCGTTTTTG+TGG | - | Chr1:39268847-39268866 | MsG0180002499.01.T01:CDS | 35.0% |
| !! | TGGCTATTTTCAGATATACC+TGG | + | Chr1:39268933-39268952 | None:intergenic | 35.0% |
| !! | TTTTCGTTTTTGTGGCCTTT+CGG | - | Chr1:39268855-39268874 | MsG0180002499.01.T01:CDS | 35.0% |
| !!! | GGCATTGATGCTCTTTTTAA+TGG | - | Chr1:39268483-39268502 | MsG0180002499.01.T01:CDS | 35.0% |
| AACAAAGGAGCAGTAAACCT+TGG | + | Chr1:39268420-39268439 | None:intergenic | 40.0% | |
| AACAGCTTTCCGCTAATGAT+CGG | - | Chr1:39268580-39268599 | MsG0180002499.01.T01:CDS | 40.0% | |
| AATGGCAGATAGGGAACAAT+CGG | - | Chr1:39268368-39268387 | MsG0180002499.01.T01:CDS | 40.0% | |
| CCTTGTCTAGAAAACCTGAA+TGG | + | Chr1:39268462-39268481 | None:intergenic | 40.0% | |
| CTAGAATTAGAAGAGCCGAA+AGG | + | Chr1:39268873-39268892 | None:intergenic | 40.0% | |
| CTATTGTGAAGATATCGTCG+AGG | - | Chr1:39268646-39268665 | MsG0180002499.01.T01:CDS | 40.0% | |
| CTCGACGATATCTTCACAAT+AGG | + | Chr1:39268648-39268667 | None:intergenic | 40.0% | |
| GCTCCTTTGTTAATCTATGG+TGG | - | Chr1:39268429-39268448 | MsG0180002499.01.T01:CDS | 40.0% | |
| GTGTTATATTTGCATGAGGC+CGG | - | Chr1:39268221-39268240 | MsG0180002499.01.T01:CDS | 40.0% | |
| TCTAACAGTTCTTCAACTCC+AGG | + | Chr1:39268561-39268580 | None:intergenic | 40.0% | |
| TCTGTTGTGGCAATTCTTGA+CGG | + | Chr1:39268815-39268834 | None:intergenic | 40.0% | |
| TGGCCACCATAGATTAACAA+AGG | + | Chr1:39268435-39268454 | None:intergenic | 40.0% | |
| ! | AAGGCATCAAATGGCAGATA+GGG | - | Chr1:39268359-39268378 | MsG0180002499.01.T01:CDS | 40.0% |
| ! | GATATGGTCCATTCTAGCTA+TGG | - | Chr1:39268309-39268328 | MsG0180002499.01.T01:CDS | 40.0% |
| ! | GTTAACTATTTTACCGGTCC+TGG | - | Chr1:39268540-39268559 | MsG0180002499.01.T01:CDS | 40.0% |
| ! | TGCTCTTTTTAATGGGACTC+CGG | - | Chr1:39268491-39268510 | MsG0180002499.01.T01:CDS | 40.0% |
| !! | AAAGGCATCAAATGGCAGAT+AGG | - | Chr1:39268358-39268377 | MsG0180002499.01.T01:CDS | 40.0% |
| !! | CCATTCAGGTTTTCTAGACA+AGG | - | Chr1:39268459-39268478 | MsG0180002499.01.T01:CDS | 40.0% |
| !! | TGGTGTCACAATAGGTAACT+CGG | - | Chr1:39268518-39268537 | MsG0180002499.01.T01:CDS | 40.0% |
| !! | TTCAGGTTTTCTAGACAAGG+TGG | - | Chr1:39268462-39268481 | MsG0180002499.01.T01:CDS | 40.0% |
| ACCCAGAATGTTGTGCATTC+TGG | + | Chr1:39268953-39268972 | None:intergenic | 45.0% | |
| ACCGTTTGCTCTAGTTGTGA+TGG | - | Chr1:39268264-39268283 | MsG0180002499.01.T01:CDS | 45.0% | |
| ACGTTGAAGAACTCGGACAA+AGG | - | Chr1:39268340-39268359 | MsG0180002499.01.T01:CDS | 45.0% | |
| AGAATGCACAACATTCTGGG+TGG | - | Chr1:39268952-39268971 | MsG0180002499.01.T01:CDS | 45.0% | |
| AGATAGGGAACAATCGGTCT+CGG | - | Chr1:39268374-39268393 | MsG0180002499.01.T01:CDS | 45.0% | |
| AGCCAGAATGCACAACATTC+TGG | - | Chr1:39268948-39268967 | MsG0180002499.01.T01:CDS | 45.0% | |
| AGTAAACCTTGGAGCGTGTT+CGG | + | Chr1:39268409-39268428 | None:intergenic | 45.0% | |
| ATGGTGGCCAAATTCCATTC+AGG | - | Chr1:39268445-39268464 | MsG0180002499.01.T01:CDS | 45.0% | |
| CAAGAATTGCCACAACAGAG+TGG | - | Chr1:39268816-39268835 | MsG0180002499.01.T01:CDS | 45.0% | |
| CCCATCACAACTAGAGCAAA+CGG | + | Chr1:39268268-39268287 | None:intergenic | 45.0% | |
| CCGTTTGCTCTAGTTGTGAT+GGG | - | Chr1:39268265-39268284 | MsG0180002499.01.T01:CDS | 45.0% | |
| CGTTTGCTCTAGTTGTGATG+GGG | - | Chr1:39268266-39268285 | MsG0180002499.01.T01:CDS | 45.0% | |
| GCAATTCTTGACGGCAAACA+GGG | + | Chr1:39268806-39268825 | None:intergenic | 45.0% | |
| GCCAGAATGCACAACATTCT+GGG | - | Chr1:39268949-39268968 | MsG0180002499.01.T01:CDS | 45.0% | |
| GTGGACAACGTTGAAGAACT+CGG | - | Chr1:39268333-39268352 | MsG0180002499.01.T01:CDS | 45.0% | |
| GTGTTATGCTGAACCAACCA+TGG | + | Chr1:39268780-39268799 | None:intergenic | 45.0% | |
| GTTTGCTCTAGTTGTGATGG+GGG | - | Chr1:39268267-39268286 | MsG0180002499.01.T01:CDS | 45.0% | |
| TCTATAGATGACCGTGATGC+AGG | + | Chr1:39268618-39268637 | None:intergenic | 45.0% | |
| TCTTGGCATACTGGACAATG+TGG | + | Chr1:39268687-39268706 | None:intergenic | 45.0% | |
| TGTCCACTCCATAGCTAGAA+TGG | + | Chr1:39268320-39268339 | None:intergenic | 45.0% | |
| ! | AGAAGTAATGACGGAGCAAC+TGG | - | Chr1:39268891-39268910 | MsG0180002499.01.T01:CDS | 45.0% |
| ! | GCTCTTTTTAATGGGACTCC+GGG | - | Chr1:39268492-39268511 | MsG0180002499.01.T01:CDS | 45.0% |
| !! | ACTCGGACAAAGGCATCAAA+TGG | - | Chr1:39268350-39268369 | MsG0180002499.01.T01:CDS | 45.0% |
| !! | CAAGTACTGGGGATACTCAT+TGG | - | Chr1:39268199-39268218 | MsG0180002499.01.T01:CDS | 45.0% |
| ATAGATGACCGTGATGCAGG+TGG | + | Chr1:39268615-39268634 | None:intergenic | 50.0% | |
| CAGTTCTTCAACTCCAGGAC+CGG | + | Chr1:39268556-39268575 | None:intergenic | 50.0% | |
| CCTATTGTGACACCAGTTCC+CGG | + | Chr1:39268513-39268532 | None:intergenic | 50.0% | |
| CTCCTTCTTCCACTCTGTTG+TGG | + | Chr1:39268828-39268847 | None:intergenic | 50.0% | |
| CTTTCCGCTAATGATCGGAG+AGG | - | Chr1:39268585-39268604 | MsG0180002499.01.T01:CDS | 50.0% | |
| GGCAATTCTTGACGGCAAAC+AGG | + | Chr1:39268807-39268826 | None:intergenic | 50.0% | |
| GTAAACCTTGGAGCGTGTTC+GGG | + | Chr1:39268408-39268427 | None:intergenic | 50.0% | |
| TAGATGACCGTGATGCAGGT+GGG | + | Chr1:39268614-39268633 | None:intergenic | 50.0% | |
| TGCCACAACAGAGTGGAAGA+AGG | - | Chr1:39268823-39268842 | MsG0180002499.01.T01:CDS | 50.0% | |
| TTTAATGGGACTCCGGGAAC+TGG | - | Chr1:39268498-39268517 | MsG0180002499.01.T01:CDS | 50.0% | |
| ! | GGTCCATTCTAGCTATGGAG+TGG | - | Chr1:39268314-39268333 | MsG0180002499.01.T01:CDS | 50.0% |
| AGATGACCGTGATGCAGGTG+GGG | + | Chr1:39268613-39268632 | None:intergenic | 55.0% | |
| CAATTCCCGAACACGCTCCA+AGG | - | Chr1:39268400-39268419 | MsG0180002499.01.T01:CDS | 55.0% | |
| GGAAGCAGCTCACTAACACC+AGG | - | Chr1:39268912-39268931 | MsG0180002499.01.T01:CDS | 55.0% | |
| GGGACCTCTCCGATCATTAG+CGG | + | Chr1:39268592-39268611 | None:intergenic | 55.0% | |
| TTCTTGACGGCAAACAGGGC+AGG | + | Chr1:39268802-39268821 | None:intergenic | 55.0% | |
| !! | CCGGGAACTGGTGTCACAAT+AGG | - | Chr1:39268510-39268529 | MsG0180002499.01.T01:CDS | 55.0% |
| GATGACCGTGATGCAGGTGG+GGG | + | Chr1:39268612-39268631 | None:intergenic | 60.0% | |
| TGTCTCCGTCCCAGTCGAAC+CGG | + | Chr1:39268243-39268262 | None:intergenic | 60.0% | |
| !! | TGCATGAGGCCGGTTCGACT+GGG | - | Chr1:39268231-39268250 | MsG0180002499.01.T01:CDS | 60.0% |
| !! | TTGCATGAGGCCGGTTCGAC+TGG | - | Chr1:39268230-39268249 | MsG0180002499.01.T01:CDS | 60.0% |
| GAGGTCCCCCACCTGCATCA+CGG | - | Chr1:39268604-39268623 | MsG0180002499.01.T01:CDS | 65.0% | |
| !! | TGAGGCCGGTTCGACTGGGA+CGG | - | Chr1:39268235-39268254 | MsG0180002499.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 39268195 | 39268986 | 39268195 | ID=MsG0180002499.01;Name=MsG0180002499.01 |
| Chr1 | mRNA | 39268195 | 39268986 | 39268195 | ID=MsG0180002499.01.T01;Parent=MsG0180002499.01;Name=MsG0180002499.01.T01;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|1|1|0|263 |
| Chr1 | exon | 39268195 | 39268986 | 39268195 | ID=MsG0180002499.01.T01:exon:7981;Parent=MsG0180002499.01.T01 |
| Chr1 | CDS | 39268195 | 39268986 | 39268195 | ID=MsG0180002499.01.T01:cds;Parent=MsG0180002499.01.T01 |
| Gene Sequence |
| Protein sequence |
