AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003497.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003497.01.T01 MTR_1g063060 66.410 390 57 6 68 383 1 390 2.80e-163 463
MsG0180003497.01.T01 MTR_1g063060 66.410 390 57 6 68 383 19 408 6.05e-163 463
MsG0180003497.01.T01 MTR_7g102120 62.051 390 74 5 68 383 1 390 2.65e-153 438
MsG0180003497.01.T01 MTR_7g110310 56.995 386 92 5 69 380 3 388 6.88e-138 399
MsG0180003497.01.T01 MTR_2g046710 57.513 386 90 5 69 380 4 389 2.49e-137 397
MsG0180003497.01.T01 MTR_4g123810 58.031 386 88 6 69 380 4 389 3.20e-137 397
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003497.01.T01 AT2G36880 61.026 390 78 6 68 383 1 390 2.73e-150 430
MsG0180003497.01.T01 AT2G36880 61.026 390 78 6 68 383 1 390 2.73e-150 430
MsG0180003497.01.T01 AT4G01850 57.623 387 90 4 68 380 1 387 3.29e-140 405
MsG0180003497.01.T01 AT4G01850 57.623 387 90 4 68 380 1 387 3.29e-140 405
MsG0180003497.01.T01 AT3G17390 58.140 387 88 5 68 380 1 387 7.62e-140 404
MsG0180003497.01.T01 AT1G02500 57.881 387 89 4 68 380 1 387 4.75e-138 399
MsG0180003497.01.T01 AT1G02500 57.881 387 89 4 68 380 1 387 4.75e-138 399

Find 115 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGTGCCAGAGCCACTTTC+TGG 0.200567 1:-63228433 MsG0180003497.01.T01:intron
ACTGCTGCTTACGGGCATTT+TGG 0.206540 1:-63228273 MsG0180003497.01.T01:CDS
TGCAAAAGTGTTGTCGCTTC+AGG 0.230277 1:-63228496 MsG0180003497.01.T01:CDS
AGCAAGAAGCCTGAGGAAAT+TGG 0.257775 1:-63229027 MsG0180003497.01.T01:CDS
TGAACTCCTTGAGCAATATC+AGG 0.302469 1:+63229060 None:intergenic
GCCCGTAAGCAGCAGTCTTC+TGG 0.322457 1:+63228280 None:intergenic
TCACAAGAGGTGGCAAATTC+AGG 0.332737 1:-63228305 MsG0180003497.01.T01:CDS
TAGTAGGACGGGACACTTGC+AGG 0.334216 1:-63229159 MsG0180003497.01.T01:intron
AGGATTGTGTGCACGCGAAT+AGG 0.355191 1:+63228812 None:intergenic
TATTGATACCTATGGTGGTT+GGG 0.358518 1:-63228600 MsG0180003497.01.T01:CDS
GGAGTGGTGCTTACATTGTT+AGG 0.368655 1:-63228524 MsG0180003497.01.T01:CDS
GTTGAATACAAGAATGATAA+TGG 0.375620 1:-63228845 MsG0180003497.01.T01:CDS
GGTGGACCTCATGGAGATGC+TGG 0.378198 1:-63228644 MsG0180003497.01.T01:CDS
ATCAATAATGATCTTCCTTC+CGG 0.379520 1:+63228616 None:intergenic
TTACAGATTCTGAGGTGAAT+AGG 0.390519 1:+63229347 None:intergenic
TGCTCGTCGTTGTATTGTGC+AGG 0.400285 1:-63228470 MsG0180003497.01.T01:CDS
GTGCAGGTTTCTTATGCAAT+TGG 0.405905 1:-63228454 MsG0180003497.01.T01:CDS
ACCAGAAGACTGCTGCTTAC+GGG 0.407668 1:-63228281 MsG0180003497.01.T01:CDS
TCTTGTATTCAACGGTAACT+TGG 0.410805 1:+63228855 None:intergenic
GCAAACCGCGTTCTGCTTTG+AGG 0.411382 1:+63231133 None:intergenic
ACGGGACACTTGCAGGGATT+GGG 0.412059 1:-63229152 MsG0180003497.01.T01:CDS
AAAAGGTAACAGTTATTAGC+AGG 0.416056 1:+63231211 None:intergenic
GGATTGTGTGCACGCGAATA+GGG 0.416704 1:+63228813 None:intergenic
ACCTGGTCACAGATCTTGTC+GGG 0.417397 1:+63229313 None:intergenic
TTGTCGTCAAGGTATTTAGC+TGG 0.418579 1:+63228707 None:intergenic
GACGGGACACTTGCAGGGAT+TGG 0.421264 1:-63229153 MsG0180003497.01.T01:CDS
TCAGGTAGGTTTGTGATTGG+TGG 0.432260 1:-63228662 MsG0180003497.01.T01:CDS
TGGTGGAGAGGTGCCTTCTC+AGG 0.432638 1:-63228570 MsG0180003497.01.T01:CDS
GGTGATCAAGGTCACATGTT+TGG 0.433876 1:-63229000 MsG0180003497.01.T01:CDS
ACCATAACTCCCTCAAGCCT+TGG 0.434421 1:+63228203 None:intergenic
TATGGTGGTTGGGGTGCTCA+TGG 0.437298 1:-63228590 MsG0180003497.01.T01:CDS
CCTTCAGGTAGGTTTGTGAT+TGG 0.440589 1:-63228665 MsG0180003497.01.T01:CDS
GGTTGGATCCTTTCCTGAGA+AGG 0.441186 1:+63228557 None:intergenic
ATCTTCCATCTCAAACCTTC+AGG 0.442573 1:-63228680 MsG0180003497.01.T01:CDS
TGCTCAAGCCCAAGGCTTGA+GGG 0.445480 1:-63228212 None:intergenic
ACAGTCTCCCATGTAAAATC+AGG 0.452392 1:+63228240 None:intergenic
GCAAAAGTGTTGTCGCTTCA+GGG 0.452576 1:-63228495 MsG0180003497.01.T01:CDS
CGTCGTGTTGAGTTGAAATG+AGG 0.453932 1:+63228792 None:intergenic
TCACCAGCTCCAATTTCCTC+AGG 0.463353 1:+63229018 None:intergenic
TGTGTGAGTGGCATTAGTTC+AGG 0.465165 1:+63228958 None:intergenic
CCACTCCTGTCCACCTTGGT+TGG 0.465451 1:+63228540 None:intergenic
TACCAGAAGACTGCTGCTTA+CGG 0.467085 1:-63228282 MsG0180003497.01.T01:CDS
CGTTGAAGAGGAACAAGAAA+AGG 0.472543 1:+63231194 None:intergenic
GGGTTTGTGTCTGCTGATGT+TGG 0.475633 1:-63229132 MsG0180003497.01.T01:CDS
CCATAACTCCCTCAAGCCTT+GGG 0.475898 1:+63228204 None:intergenic
GAAATTGGAGCTGGTGATCA+AGG 0.483468 1:-63229012 MsG0180003497.01.T01:CDS
TGCCACCTCTTGTGAGGTCA+AGG 0.490427 1:+63228313 None:intergenic
AGGATCCAACCAAGGTGGAC+AGG 0.494928 1:-63228545 MsG0180003497.01.T01:CDS
AGGTTGATAGCAATCATTCC+AGG 0.495973 1:+63228333 None:intergenic
TGCCATCCTTGATGCATGCT+TGG 0.499134 1:-63229284 MsG0180003497.01.T01:CDS
AGGTATTTAGCTGGGACGAC+AGG 0.499951 1:+63228716 None:intergenic
CAAGGATGGCATCTGAAACC+TGG 0.510900 1:+63229296 None:intergenic
CATGGAGATGCTGGGCTCAC+CGG 0.511049 1:-63228635 MsG0180003497.01.T01:CDS
TTATTGATACCTATGGTGGT+TGG 0.512065 1:-63228601 MsG0180003497.01.T01:CDS
AAGATCATTATTGATACCTA+TGG 0.514480 1:-63228608 MsG0180003497.01.T01:CDS
CACAAACCAGAAAGTGGCTC+TGG 0.518838 1:+63228427 None:intergenic
TTGGGGTGCTCATGGTGGAG+AGG 0.520281 1:-63228582 MsG0180003497.01.T01:CDS
CTTGTATTCAACGGTAACTT+GGG 0.524167 1:+63228856 None:intergenic
GTAACTTGGGTCTTACCATC+AGG 0.526446 1:+63228869 None:intergenic
ATTGATACCTATGGTGGTTG+GGG 0.527612 1:-63228599 MsG0180003497.01.T01:CDS
ATGCTCAAGCCCAAGGCTTG+AGG 0.527948 1:-63228213 None:intergenic
ATTATCATTCTTGTATTCAA+CGG 0.531052 1:+63228847 None:intergenic
GATATTGCTCAAGGAGTTCA+CGG 0.536055 1:-63229057 MsG0180003497.01.T01:CDS
GTGGACCTCATGGAGATGCT+GGG 0.537821 1:-63228643 MsG0180003497.01.T01:CDS
CCCCGACAAGATCTGTGACC+AGG 0.538168 1:-63229314 MsG0180003497.01.T01:CDS
TAGTTCAGGTGTTTCGTCAG+TGG 0.542460 1:+63228972 None:intergenic
TTTGTGATTGGTGGACCTCA+TGG 0.544583 1:-63228653 MsG0180003497.01.T01:CDS
TGTCGTCAAGGTATTTAGCT+GGG 0.547725 1:+63228708 None:intergenic
AGTGTCCACAAACCAGAAAG+TGG 0.551585 1:+63228421 None:intergenic
TGAAGGTTTGAGATGGAAGA+TGG 0.552463 1:+63228682 None:intergenic
ATGCCCATGGGTTAGCCTGA+TGG 0.559488 1:-63228884 MsG0180003497.01.T01:intron
TTACATCTTTCACGTTGAAG+AGG 0.562996 1:+63231182 None:intergenic
GGTGGTTGGGGTGCTCATGG+TGG 0.563645 1:-63228587 MsG0180003497.01.T01:CDS
AACCTGGTCACAGATCTTGT+CGG 0.563707 1:+63229312 None:intergenic
TGGAGCAAGATAAAGAGAGC+AGG 0.563897 1:-63229264 MsG0180003497.01.T01:CDS
ATGGCTGATTACACTATCAC+TGG 0.565339 1:-63229807 MsG0180003497.01.T01:CDS
AAGCCTGAGGAAATTGGAGC+TGG 0.569064 1:-63229021 MsG0180003497.01.T01:CDS
AGCACCACTCCTGTCCACCT+TGG 0.571010 1:+63228536 None:intergenic
CTCCAAGCATGCATCAAGGA+TGG 0.575330 1:+63229282 None:intergenic
ATCATTATTGATACCTATGG+TGG 0.575435 1:-63228605 MsG0180003497.01.T01:CDS
ATGAGCACCCCAACCACCAT+AGG 0.575628 1:+63228592 None:intergenic
CAAGAATGATAATGGAGCCA+TGG 0.577331 1:-63228837 MsG0180003497.01.T01:CDS
GGAAGATGGTCTTGTCGTCA+AGG 0.577435 1:+63228696 None:intergenic
TGTGAAGATGCTCAAGCCCA+AGG 0.581845 1:-63228220 MsG0180003497.01.T01:CDS
ACCTTCGTTTACAGATTCTG+AGG 0.589403 1:+63229339 None:intergenic
AACCTTGACCTCACAAGAGG+TGG 0.593643 1:-63228315 MsG0180003497.01.T01:CDS
TGAATTTGCCACCTCTTGTG+AGG 0.598537 1:+63228307 None:intergenic
ACCTACCTGAAGGTTTGAGA+TGG 0.600743 1:+63228675 None:intergenic
CAGAGCCCTGATATTGCTCA+AGG 0.602611 1:-63229066 MsG0180003497.01.T01:CDS
CTTGCTCCAAGCATGCATCA+AGG 0.602878 1:+63229278 None:intergenic
AAAGGTAACAGTTATTAGCA+GGG 0.604330 1:+63231212 None:intergenic
GAGATGCTGGGCTCACCGGA+AGG 0.606502 1:-63228631 MsG0180003497.01.T01:CDS
GAACTCCTTGAGCAATATCA+GGG 0.613163 1:+63229061 None:intergenic
AGTAGGACGGGACACTTGCA+GGG 0.618819 1:-63229158 MsG0180003497.01.T01:intron
AGTGATAGTGTAATCAGCCA+TGG 0.618902 1:+63229809 None:intergenic
AGTGGCAGCACATGTGTGAG+TGG 0.628530 1:+63228946 None:intergenic
GAGAGGTGCCTTCTCAGGAA+AGG 0.629679 1:-63228565 MsG0180003497.01.T01:CDS
ATCAACCTTGACCTCACAAG+AGG 0.632107 1:-63228318 MsG0180003497.01.T01:CDS
AGATTCTGAGGTGAATAGGA+AGG 0.635764 1:+63229351 None:intergenic
TCCATCTCAAACCTTCAGGT+AGG 0.636063 1:-63228676 MsG0180003497.01.T01:CDS
CCAATCACAAACCTACCTGA+AGG 0.638421 1:+63228665 None:intergenic
AATCAGCCATGGTTTGAACT+CGG 0.640241 1:+63229820 None:intergenic
CCAACCAAGGTGGACAGGAG+TGG 0.650759 1:-63228540 MsG0180003497.01.T01:CDS
ACCTCAGAATCTGTAAACGA+AGG 0.651225 1:-63229340 MsG0180003497.01.T01:CDS
GGCTGTGAAGGTACTCGCCG+CGG 0.668242 1:-63231260 MsG0180003497.01.T01:CDS
ATGGACGGAGATGGCTGTGA+AGG 0.670060 1:-63231272 None:intergenic
CTCAGGAAAGGATCCAACCA+AGG 0.675495 1:-63228553 MsG0180003497.01.T01:CDS
TCACTTGAGCAAGAAGCCTG+AGG 0.682617 1:-63229034 MsG0180003497.01.T01:CDS
GTGAGCCCAGCATCTCCATG+AGG 0.687695 1:+63228638 None:intergenic
GTGCACGCGAATAGGGACCA+TGG 0.688328 1:+63228820 None:intergenic
AGGAAAGGATCCAACCAAGG+TGG 0.705983 1:-63228550 MsG0180003497.01.T01:CDS
CCTGGTCACAGATCTTGTCG+GGG 0.713673 1:+63229314 None:intergenic
AGACACCTCAAAGCAGAACG+CGG 0.718110 1:-63231138 MsG0180003497.01.T01:intron
TAACAGACTAAGTTTCTCCG+CGG 0.726303 1:+63231243 None:intergenic
GGAGTGCCGAGTTCAAACCA+TGG 0.731624 1:-63229826 MsG0180003497.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGTTATATGTTATTAATT+TGG + Chr1:63229072-63229091 None:intergenic 10.0%
!!! AAAATAAAGTTTTAACAAAA+TGG - Chr1:63228423-63228442 MsG0180003497.01.T01:intron 10.0%
!!! TTTTTTAAGCTATATTTTTT+TGG + Chr1:63228515-63228534 None:intergenic 10.0%
!! AAACCTTTATTATATGAATA+TGG + Chr1:63229560-63229579 None:intergenic 15.0%
!! AATCCATATTCATATAATAA+AGG - Chr1:63229554-63229573 MsG0180003497.01.T01:intron 15.0%
!!! AAATTTTACAGAATTCATTA+GGG + Chr1:63229715-63229734 None:intergenic 15.0%
!!! CAATAATATTTTGTTTGTTT+TGG - Chr1:63229786-63229805 MsG0180003497.01.T01:intron 15.0%
!! ACATCTAATATATATACTCT+TGG - Chr1:63230072-63230091 MsG0180003497.01.T01:intron 20.0%
!! ATTATCATTCTTGTATTCAA+CGG + Chr1:63230633-63230652 None:intergenic 20.0%
!! GTCAAATATTTATGATCTAA+AGG - Chr1:63229484-63229503 MsG0180003497.01.T01:intron 20.0%
!! TAAAAAAGTTACCGTAAAAA+AGG - Chr1:63228539-63228558 MsG0180003497.01.T01:CDS 20.0%
!!! AAAAAAAAGTGCGAAAAAAA+AGG - Chr1:63228612-63228631 MsG0180003497.01.T01:CDS 20.0%
!!! CAAATTTTACAGAATTCATT+AGG + Chr1:63229716-63229735 None:intergenic 20.0%
!!! TTCTTTTGTAATTGTGAATT+TGG - Chr1:63228882-63228901 MsG0180003497.01.T01:intron 20.0%
!!! TTTTTTTTTGGAATCAGATA+TGG - Chr1:63230100-63230119 MsG0180003497.01.T01:intron 20.0%
! AAGATCATTATTGATACCTA+TGG - Chr1:63230869-63230888 MsG0180003497.01.T01:intron 25.0%
! AAGGAAAGAAAATGCATAAA+TGG - Chr1:63228558-63228577 MsG0180003497.01.T01:CDS 25.0%
! AGAAAATCAAGATTAAGCTA+TGG + Chr1:63228952-63228971 None:intergenic 25.0%
! ATGATTATCTGTATCTGATA+TGG - Chr1:63229216-63229235 MsG0180003497.01.T01:intron 25.0%
! GTTGAATACAAGAATGATAA+TGG - Chr1:63230632-63230651 MsG0180003497.01.T01:intron 25.0%
! TACAAAAACTAACATGGTTA+TGG - Chr1:63230252-63230271 MsG0180003497.01.T01:intron 25.0%
! TTGTTAGATCTAAAGAGAAT+AGG + Chr1:63228825-63228844 None:intergenic 25.0%
!! ATGATAATGTGGACTTATAT+TGG + Chr1:63228703-63228722 None:intergenic 25.0%
!! CTAACAATATCTAGTTTCTA+TGG - Chr1:63228838-63228857 MsG0180003497.01.T01:CDS 25.0%
!!! ATGTTTTTAGTCTAAACAAG+TGG - Chr1:63229584-63229603 MsG0180003497.01.T01:intron 25.0%
!!! CTCTTGGTCTTATTTTTTTT+TGG - Chr1:63230088-63230107 MsG0180003497.01.T01:intron 25.0%
!!! GCATTTTCTTTCCTTTTTTA+CGG + Chr1:63228553-63228572 None:intergenic 25.0%
AAAAAAAAGGAAGAGATGGA+GGG - Chr1:63228625-63228644 MsG0180003497.01.T01:CDS 30.0%
AAAAGGTAACAGTTATTAGC+AGG + Chr1:63228269-63228288 None:intergenic 30.0%
AAACTTACATACAGTACCAT+AGG + Chr1:63228489-63228508 None:intergenic 30.0%
AAAGGTAACAGTTATTAGCA+GGG + Chr1:63228268-63228287 None:intergenic 30.0%
AAGAGTAAAAACAGTACCTA+TGG - Chr1:63228470-63228489 MsG0180003497.01.T01:CDS 30.0%
AATGTGGACTTATATTGGAT+TGG + Chr1:63228698-63228717 None:intergenic 30.0%
ACTATGAGAAAATAGTAGGA+CGG - Chr1:63230306-63230325 MsG0180003497.01.T01:intron 30.0%
AGAACCAAAATATCCTTATC+TGG + Chr1:63231100-63231119 None:intergenic 30.0%
AGAAGAATGTGAAATGAAAG+AGG + Chr1:63228731-63228750 None:intergenic 30.0%
ATCAATAATGATCTTCCTTC+CGG + Chr1:63230864-63230883 None:intergenic 30.0%
ATCATTATTGATACCTATGG+TGG - Chr1:63230872-63230891 MsG0180003497.01.T01:intron 30.0%
GAATTATTTCCTTTGCATAG+TGG - Chr1:63229943-63229962 MsG0180003497.01.T01:intron 30.0%
! AAGAGGAAGAAATGATAATG+TGG + Chr1:63228714-63228733 None:intergenic 30.0%
! ATTAAGGAGAATTTGACTTC+AGG - Chr1:63231121-63231140 MsG0180003497.01.T01:intron 30.0%
! ATTCAACACTTTCTTTTAGC+GGG - Chr1:63229867-63229886 MsG0180003497.01.T01:intron 30.0%
! CAATTGACTACAGATTTTGA+AGG + Chr1:63230033-63230052 None:intergenic 30.0%
! CATTCAACACTTTCTTTTAG+CGG - Chr1:63229866-63229885 MsG0180003497.01.T01:intron 30.0%
! GATTCCAGATAAGGATATTT+TGG - Chr1:63231093-63231112 MsG0180003497.01.T01:intron 30.0%
! GTTTTTGAATTGCGATTGTA+TGG - Chr1:63228977-63228996 MsG0180003497.01.T01:CDS 30.0%
! TTATATTGGATTGGAAAGAG+AGG + Chr1:63228689-63228708 None:intergenic 30.0%
!! CTTTTTAAGCAACAGATCAA+AGG - Chr1:63229834-63229853 MsG0180003497.01.T01:intron 30.0%
!!! AACCATGTTAGTTTTTGTAC+AGG + Chr1:63230251-63230270 None:intergenic 30.0%
!!! GGATATTTTGGTTCTGATTA+AGG - Chr1:63231105-63231124 MsG0180003497.01.T01:intron 30.0%
!!! TGTTTTGGTAAACAAGAAGT+TGG - Chr1:63229801-63229820 MsG0180003497.01.T01:CDS 30.0%
AACTGGAAAGATTCCAGATA+AGG - Chr1:63231084-63231103 MsG0180003497.01.T01:intron 35.0%
AATTTGGACTCGTTATCGTA+TGG - Chr1:63228898-63228917 MsG0180003497.01.T01:intron 35.0%
ACTAACATGGTTATGGTCTT+TGG - Chr1:63230259-63230278 MsG0180003497.01.T01:intron 35.0%
AGTCTAAACAAGTGGTTTAG+TGG - Chr1:63229592-63229611 MsG0180003497.01.T01:intron 35.0%
CTATGAGAAAATAGTAGGAC+GGG - Chr1:63230307-63230326 MsG0180003497.01.T01:intron 35.0%
CTCAAGTGGTTAATGAACTT+TGG - Chr1:63229620-63229639 MsG0180003497.01.T01:intron 35.0%
CTCTCAATTCAATCACACAA+AGG - Chr1:63228763-63228782 MsG0180003497.01.T01:CDS 35.0%
CTTACATTACCACTATGCAA+AGG + Chr1:63229955-63229974 None:intergenic 35.0%
CTTGTATTCAACGGTAACTT+GGG + Chr1:63230624-63230643 None:intergenic 35.0%
GAAAAAAAAGGAAGAGATGG+AGG - Chr1:63228624-63228643 MsG0180003497.01.T01:CDS 35.0%
GACCTGTACAAAAACTAACA+TGG - Chr1:63230246-63230265 MsG0180003497.01.T01:intron 35.0%
GTCTTTGGTGAAATCACAAA+AGG - Chr1:63230274-63230293 MsG0180003497.01.T01:intron 35.0%
TAATGAACTTTGGACCAGTT+GGG - Chr1:63229630-63229649 MsG0180003497.01.T01:intron 35.0%
TATTGATACCTATGGTGGTT+GGG - Chr1:63230877-63230896 MsG0180003497.01.T01:intron 35.0%
TCTTGTATTCAACGGTAACT+TGG + Chr1:63230625-63230644 None:intergenic 35.0%
TGCGAAAAAAAAGGAAGAGA+TGG - Chr1:63228621-63228640 MsG0180003497.01.T01:CDS 35.0%
TTAATGAACTTTGGACCAGT+TGG - Chr1:63229629-63229648 MsG0180003497.01.T01:intron 35.0%
TTACATCTTTCACGTTGAAG+AGG + Chr1:63228298-63228317 None:intergenic 35.0%
TTATTGATACCTATGGTGGT+TGG - Chr1:63230876-63230895 MsG0180003497.01.T01:intron 35.0%
TTTGTGGACACTTACAAAAC+TGG - Chr1:63231067-63231086 MsG0180003497.01.T01:intron 35.0%
! GTGGACTATGAGAAAATAGT+AGG - Chr1:63230302-63230321 MsG0180003497.01.T01:intron 35.0%
! TGACGATCCTGATTTTACAT+GGG - Chr1:63231230-63231249 MsG0180003497.01.T01:CDS 35.0%
! TTCAACACTTTCTTTTAGCG+GGG - Chr1:63229868-63229887 MsG0180003497.01.T01:intron 35.0%
! TTTGCGATTCTAAGAGAACA+GGG - Chr1:63228362-63228381 MsG0180003497.01.T01:intron 35.0%
!! GACACGTGTTGATGTTTTAT+TGG - Chr1:63228445-63228464 MsG0180003497.01.T01:CDS 35.0%
!! TTACAGATTCTGAGGTGAAT+AGG + Chr1:63230133-63230152 None:intergenic 35.0%
AATCAGCCATGGTTTGAACT+CGG + Chr1:63229660-63229679 None:intergenic 40.0%
ACAGTCTCCCATGTAAAATC+AGG + Chr1:63231240-63231259 None:intergenic 40.0%
ACATGTGCTGCCACTAAAAT+TGG - Chr1:63230539-63230558 MsG0180003497.01.T01:intron 40.0%
ACCTCAGAATCTGTAAACGA+AGG - Chr1:63230137-63230156 MsG0180003497.01.T01:intron 40.0%
ACCTTCGTTTACAGATTCTG+AGG + Chr1:63230141-63230160 None:intergenic 40.0%
AGAAGAATAAGACATGCCCA+TGG - Chr1:63230580-63230599 MsG0180003497.01.T01:intron 40.0%
ATCTTCCATCTCAAACCTTC+AGG - Chr1:63230797-63230816 MsG0180003497.01.T01:intron 40.0%
ATGGCTGATTACACTATCAC+TGG - Chr1:63229670-63229689 MsG0180003497.01.T01:intron 40.0%
ATTGATACCTATGGTGGTTG+GGG - Chr1:63230878-63230897 MsG0180003497.01.T01:intron 40.0%
CGTTGAAGAGGAACAAGAAA+AGG + Chr1:63228286-63228305 None:intergenic 40.0%
GAAATCACAAAAGGCAAAGG+TGG - Chr1:63230283-63230302 MsG0180003497.01.T01:intron 40.0%
GAACTCCTTGAGCAATATCA+GGG + Chr1:63230419-63230438 None:intergenic 40.0%
GAAGAATAAGACATGCCCAT+GGG - Chr1:63230581-63230600 MsG0180003497.01.T01:intron 40.0%
GATATTGCTCAAGGAGTTCA+CGG - Chr1:63230420-63230439 MsG0180003497.01.T01:intron 40.0%
GGTGAAATCACAAAAGGCAA+AGG - Chr1:63230280-63230299 MsG0180003497.01.T01:intron 40.0%
GTGCAGGTTTCTTATGCAAT+TGG - Chr1:63231023-63231042 MsG0180003497.01.T01:intron 40.0%
GTTTAGTGGTTGAACTCAAG+TGG - Chr1:63229606-63229625 MsG0180003497.01.T01:intron 40.0%
TAACAGACTAAGTTTCTCCG+CGG + Chr1:63228237-63228256 None:intergenic 40.0%
TGAACTCCTTGAGCAATATC+AGG + Chr1:63230420-63230439 None:intergenic 40.0%
TTCTTCTAACTTCAGTGAGC+TGG + Chr1:63230567-63230586 None:intergenic 40.0%
! AGTGATAGTGTAATCAGCCA+TGG + Chr1:63229671-63229690 None:intergenic 40.0%
! GTGACGATCCTGATTTTACA+TGG - Chr1:63231229-63231248 MsG0180003497.01.T01:CDS 40.0%
! GTTTGCGATTCTAAGAGAAC+AGG - Chr1:63228361-63228380 MsG0180003497.01.T01:intron 40.0%
! TCAACACTTTCTTTTAGCGG+GGG - Chr1:63229869-63229888 MsG0180003497.01.T01:intron 40.0%
! TGAAGGTTTGAGATGGAAGA+TGG + Chr1:63230798-63230817 None:intergenic 40.0%
! TGTCGTCAAGGTATTTAGCT+GGG + Chr1:63230772-63230791 None:intergenic 40.0%
! TTGCGATTCTAAGAGAACAG+GGG - Chr1:63228363-63228382 MsG0180003497.01.T01:intron 40.0%
! TTGTCGTCAAGGTATTTAGC+TGG + Chr1:63230773-63230792 None:intergenic 40.0%
!! AGATTCTGAGGTGAATAGGA+AGG + Chr1:63230129-63230148 None:intergenic 40.0%
!! AGGTTGATAGCAATCATTCC+AGG + Chr1:63231147-63231166 None:intergenic 40.0%
!! CAAGAATGATAATGGAGCCA+TGG - Chr1:63230640-63230659 MsG0180003497.01.T01:intron 40.0%
AAAAAGGAAGAGATGGAGGG+AGG - Chr1:63228628-63228647 MsG0180003497.01.T01:CDS 45.0%
AACCTGGTCACAGATCTTGT+CGG + Chr1:63230168-63230187 None:intergenic 45.0%
ACCTACCTGAAGGTTTGAGA+TGG + Chr1:63230805-63230824 None:intergenic 45.0%
AGAGGAGAGAGAAACGCATA+CGG + Chr1:63228671-63228690 None:intergenic 45.0%
AGCAAGAAGCCTGAGGAAAT+TGG - Chr1:63230450-63230469 MsG0180003497.01.T01:intron 45.0%
AGCTATGGATCTGCGAATGT+AGG + Chr1:63228937-63228956 None:intergenic 45.0%
AGTGTCCACAAACCAGAAAG+TGG + Chr1:63231059-63231078 None:intergenic 45.0%
ATCAACCTTGACCTCACAAG+AGG - Chr1:63231159-63231178 MsG0180003497.01.T01:CDS 45.0%
CCAATCACAAACCTACCTGA+AGG + Chr1:63230815-63230834 None:intergenic 45.0%
CCTTCAGGTAGGTTTGTGAT+TGG - Chr1:63230812-63230831 MsG0180003497.01.T01:intron 45.0%
GAAATTGGAGCTGGTGATCA+AGG - Chr1:63230465-63230484 MsG0180003497.01.T01:intron 45.0%
GGAGTGGTGCTTACATTGTT+AGG - Chr1:63230953-63230972 MsG0180003497.01.T01:intron 45.0%
GGTGATCAAGGTCACATGTT+TGG - Chr1:63230477-63230496 MsG0180003497.01.T01:intron 45.0%
GTAACTTGGGTCTTACCATC+AGG + Chr1:63230611-63230630 None:intergenic 45.0%
TAGTTCAGGTGTTTCGTCAG+TGG + Chr1:63230508-63230527 None:intergenic 45.0%
TCACAAGAGGTGGCAAATTC+AGG - Chr1:63231172-63231191 MsG0180003497.01.T01:CDS 45.0%
TCAGGTAGGTTTGTGATTGG+TGG - Chr1:63230815-63230834 MsG0180003497.01.T01:intron 45.0%
TCCATCTCAAACCTTCAGGT+AGG - Chr1:63230801-63230820 MsG0180003497.01.T01:intron 45.0%
TGAATTTGCCACCTCTTGTG+AGG + Chr1:63231173-63231192 None:intergenic 45.0%
TGGAGCAAGATAAAGAGAGC+AGG - Chr1:63230213-63230232 MsG0180003497.01.T01:intron 45.0%
TGTGTGAGTGGCATTAGTTC+AGG + Chr1:63230522-63230541 None:intergenic 45.0%
TTACCATCAGGCTAACCCAT+GGG + Chr1:63230599-63230618 None:intergenic 45.0%
! TACCAGAAGACTGCTGCTTA+CGG - Chr1:63231195-63231214 MsG0180003497.01.T01:CDS 45.0%
! TGAGCTGGCACCAATTTTAG+TGG + Chr1:63230552-63230571 None:intergenic 45.0%
! TTTGTGATTGGTGGACCTCA+TGG - Chr1:63230824-63230843 MsG0180003497.01.T01:intron 45.0%
!! CGTCGTGTTGAGTTGAAATG+AGG + Chr1:63230688-63230707 None:intergenic 45.0%
!! GCAAAAGTGTTGTCGCTTCA+GGG - Chr1:63230982-63231001 MsG0180003497.01.T01:intron 45.0%
!! TGCAAAAGTGTTGTCGCTTC+AGG - Chr1:63230981-63231000 MsG0180003497.01.T01:intron 45.0%
!!! TTTATTTTAATTTATTTTGA+CGG + Chr1:63228411-63228430 None:intergenic 5.0%
AACCTTGACCTCACAAGAGG+TGG - Chr1:63231162-63231181 MsG0180003497.01.T01:CDS 50.0%
AAGCCTGAGGAAATTGGAGC+TGG - Chr1:63230456-63230475 MsG0180003497.01.T01:intron 50.0%
ACCTGGTCACAGATCTTGTC+GGG + Chr1:63230167-63230186 None:intergenic 50.0%
AGACACCTCAAAGCAGAACG+CGG - Chr1:63228339-63228358 MsG0180003497.01.T01:intron 50.0%
AGGAAAGGATCCAACCAAGG+TGG - Chr1:63230927-63230946 MsG0180003497.01.T01:intron 50.0%
AGGATTGTGTGCACGCGAAT+AGG + Chr1:63230668-63230687 None:intergenic 50.0%
AGGTATTTAGCTGGGACGAC+AGG + Chr1:63230764-63230783 None:intergenic 50.0%
CACAAACCAGAAAGTGGCTC+TGG + Chr1:63231053-63231072 None:intergenic 50.0%
CAGAGCCCTGATATTGCTCA+AGG - Chr1:63230411-63230430 MsG0180003497.01.T01:intron 50.0%
CTATCACTGGCAGTGCATGT+CGG - Chr1:63229683-63229702 MsG0180003497.01.T01:intron 50.0%
CTCAGGAAAGGATCCAACCA+AGG - Chr1:63230924-63230943 MsG0180003497.01.T01:intron 50.0%
CTCCAAGCATGCATCAAGGA+TGG + Chr1:63230198-63230217 None:intergenic 50.0%
CTTACCATCAGGCTAACCCA+TGG + Chr1:63230600-63230619 None:intergenic 50.0%
CTTGCTCCAAGCATGCATCA+AGG + Chr1:63230202-63230221 None:intergenic 50.0%
GGATTGTGTGCACGCGAATA+GGG + Chr1:63230667-63230686 None:intergenic 50.0%
GGGTTTGTGTCTGCTGATGT+TGG - Chr1:63230345-63230364 MsG0180003497.01.T01:intron 50.0%
GGTTGGATCCTTTCCTGAGA+AGG + Chr1:63230923-63230942 None:intergenic 50.0%
TCACCAGCTCCAATTTCCTC+AGG + Chr1:63230462-63230481 None:intergenic 50.0%
TCACTTGAGCAAGAAGCCTG+AGG - Chr1:63230443-63230462 MsG0180003497.01.T01:intron 50.0%
TGCCATCCTTGATGCATGCT+TGG - Chr1:63230193-63230212 MsG0180003497.01.T01:intron 50.0%
TGCTCGTCGTTGTATTGTGC+AGG - Chr1:63231007-63231026 MsG0180003497.01.T01:intron 50.0%
TGTGAAGATGCTCAAGCCCA+AGG - Chr1:63231257-63231276 MsG0180003497.01.T01:CDS 50.0%
! ACACTTTCTTTTAGCGGGGG+TGG - Chr1:63229872-63229891 MsG0180003497.01.T01:intron 50.0%
! ACCAGAAGACTGCTGCTTAC+GGG - Chr1:63231196-63231215 MsG0180003497.01.T01:CDS 50.0%
! ACTTTCTTTTAGCGGGGGTG+GGG - Chr1:63229874-63229893 MsG0180003497.01.T01:intron 50.0%
! AGTTTTTGTACAGGTCGCGC+AGG + Chr1:63230242-63230261 None:intergenic 50.0%
! CAAGGATGGCATCTGAAACC+TGG + Chr1:63230184-63230203 None:intergenic 50.0%
! CACTTTCTTTTAGCGGGGGT+GGG - Chr1:63229873-63229892 MsG0180003497.01.T01:intron 50.0%
! GGAGAATTTGACTTCAGGCC+TGG - Chr1:63231126-63231145 MsG0180003497.01.T01:intron 50.0%
!! ACTGCTGCTTACGGGCATTT+TGG - Chr1:63231204-63231223 MsG0180003497.01.T01:CDS 50.0%
!! CAGAGCCACTTTCTGGTTTG+TGG - Chr1:63231051-63231070 MsG0180003497.01.T01:intron 50.0%
!! GGAAGATGGTCTTGTCGTCA+AGG + Chr1:63230784-63230803 None:intergenic 50.0%
ACGGGACACTTGCAGGGATT+GGG - Chr1:63230325-63230344 MsG0180003497.01.T01:intron 55.0%
AGGATCCAACCAAGGTGGAC+AGG - Chr1:63230932-63230951 MsG0180003497.01.T01:intron 55.0%
AGTAGGACGGGACACTTGCA+GGG - Chr1:63230319-63230338 MsG0180003497.01.T01:intron 55.0%
AGTGGCAGCACATGTGTGAG+TGG + Chr1:63230534-63230553 None:intergenic 55.0%
ATGAGCACCCCAACCACCAT+AGG + Chr1:63230888-63230907 None:intergenic 55.0%
CCTGGTCACAGATCTTGTCG+GGG + Chr1:63230166-63230185 None:intergenic 55.0%
GCAAACCGCGTTCTGCTTTG+AGG + Chr1:63228347-63228366 None:intergenic 55.0%
GTGGACCTCATGGAGATGCT+GGG - Chr1:63230834-63230853 MsG0180003497.01.T01:intron 55.0%
TAGTAGGACGGGACACTTGC+AGG - Chr1:63230318-63230337 MsG0180003497.01.T01:intron 55.0%
TGCCACCTCTTGTGAGGTCA+AGG + Chr1:63231167-63231186 None:intergenic 55.0%
TTGAACTCGGCACTCCCAAC+TGG + Chr1:63229647-63229666 None:intergenic 55.0%
! CTTTCTTTTAGCGGGGGTGG+GGG - Chr1:63229875-63229894 MsG0180003497.01.T01:intron 55.0%
! TATGGTGGTTGGGGTGCTCA+TGG - Chr1:63230887-63230906 MsG0180003497.01.T01:intron 55.0%
!! ATGCCCATGGGTTAGCCTGA+TGG - Chr1:63230593-63230612 MsG0180003497.01.T01:intron 55.0%
!! GAGAGGTGCCTTCTCAGGAA+AGG - Chr1:63230912-63230931 MsG0180003497.01.T01:intron 55.0%
!! GGAGTGCCGAGTTCAAACCA+TGG - Chr1:63229651-63229670 MsG0180003497.01.T01:intron 55.0%
AGCACCACTCCTGTCCACCT+TGG + Chr1:63230944-63230963 None:intergenic 60.0%
CATGGAGATGCTGGGCTCAC+CGG - Chr1:63230842-63230861 MsG0180003497.01.T01:intron 60.0%
CCAACCAAGGTGGACAGGAG+TGG - Chr1:63230937-63230956 MsG0180003497.01.T01:intron 60.0%
CCACTCCTGTCCACCTTGGT+TGG + Chr1:63230940-63230959 None:intergenic 60.0%
CCCCGACAAGATCTGTGACC+AGG - Chr1:63230163-63230182 MsG0180003497.01.T01:intron 60.0%
GACGGGACACTTGCAGGGAT+TGG - Chr1:63230324-63230343 MsG0180003497.01.T01:intron 60.0%
GCCCGTAAGCAGCAGTCTTC+TGG + Chr1:63231200-63231219 None:intergenic 60.0%
GGTGGACCTCATGGAGATGC+TGG - Chr1:63230833-63230852 MsG0180003497.01.T01:intron 60.0%
GTGAGCCCAGCATCTCCATG+AGG + Chr1:63230842-63230861 None:intergenic 60.0%
GTGCACGCGAATAGGGACCA+TGG + Chr1:63230660-63230679 None:intergenic 60.0%
! GGAGTGCCAGAGCCACTTTC+TGG - Chr1:63231044-63231063 MsG0180003497.01.T01:intron 60.0%
! TTGGGGTGCTCATGGTGGAG+AGG - Chr1:63230895-63230914 MsG0180003497.01.T01:intron 60.0%
!! TGGTGGAGAGGTGCCTTCTC+AGG - Chr1:63230907-63230926 MsG0180003497.01.T01:intron 60.0%
GAGATGCTGGGCTCACCGGA+AGG - Chr1:63230846-63230865 MsG0180003497.01.T01:intron 65.0%
! GGTGGTTGGGGTGCTCATGG+TGG - Chr1:63230890-63230909 MsG0180003497.01.T01:intron 65.0%
! TCTTTTAGCGGGGGTGGGGG+TGG - Chr1:63229878-63229897 MsG0180003497.01.T01:intron 65.0%
!! GGCTGTGAAGGTACTCGCCG+CGG - Chr1:63228217-63228236 MsG0180003497.01.T01:CDS 65.0%
TTAGCGGGGGTGGGGGTGGA+GGG - Chr1:63229882-63229901 MsG0180003497.01.T01:intron 70.0%
TTTAGCGGGGGTGGGGGTGG+AGG - Chr1:63229881-63229900 MsG0180003497.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 63228215 63231284 63228215 ID=MsG0180003497.01;Name=MsG0180003497.01
Chr1 mRNA 63228215 63231284 63228215 ID=MsG0180003497.01.T01;Parent=MsG0180003497.01;Name=MsG0180003497.01.T01;_AED=0.49;_eAED=0.58;_QI=0|0|0|1|0.6|0.66|6|0|383
Chr1 exon 63231139 63231284 63231139 ID=MsG0180003497.01.T01:exon:50060;Parent=MsG0180003497.01.T01
Chr1 exon 63229799 63229851 63229799 ID=MsG0180003497.01.T01:exon:50059;Parent=MsG0180003497.01.T01
Chr1 exon 63229249 63229382 63229249 ID=MsG0180003497.01.T01:exon:50058;Parent=MsG0180003497.01.T01
Chr1 exon 63228957 63229175 63228957 ID=MsG0180003497.01.T01:exon:50057;Parent=MsG0180003497.01.T01
Chr1 exon 63228434 63228891 63228434 ID=MsG0180003497.01.T01:exon:50056;Parent=MsG0180003497.01.T01
Chr1 exon 63228215 63228356 63228215 ID=MsG0180003497.01.T01:exon:50055;Parent=MsG0180003497.01.T01
Chr1 CDS 63231139 63231284 63231139 ID=MsG0180003497.01.T01:cds;Parent=MsG0180003497.01.T01
Chr1 CDS 63229799 63229851 63229799 ID=MsG0180003497.01.T01:cds;Parent=MsG0180003497.01.T01
Chr1 CDS 63229249 63229382 63229249 ID=MsG0180003497.01.T01:cds;Parent=MsG0180003497.01.T01
Chr1 CDS 63228957 63229175 63228957 ID=MsG0180003497.01.T01:cds;Parent=MsG0180003497.01.T01
Chr1 CDS 63228434 63228891 63228434 ID=MsG0180003497.01.T01:cds;Parent=MsG0180003497.01.T01
Chr1 CDS 63228215 63228356 63228215 ID=MsG0180003497.01.T01:cds;Parent=MsG0180003497.01.T01
Gene Sequence

>MsG0180003497.01.T01

ATGGCTGTGAAGGTACTCGCCGCGGAGAAACTTAGTCTGTTAGCTATGAACCCTGCTAATAACTGTTACCTTTTCTTGTTCCTCTTCAACGTGAAAGATGTAAAATCGCATGTGTTGAAGAGACAGACACCTCAAAGCAGAACGCGTTGGGAGTGCCGAGTTCAAACCATGGCTGATTACACTATCACTGGCAGTGCATATATGGATACCTTCCTATTCACCTCAGAATCTGTAAACGAAGGTCACCCCGACAAGATCTGTGACCAGGTTTCAGATGCCATCCTTGATGCATGCTTGGAGCAAGATAAAGAGAGCAGGTTGCCTGCGCGACCTGACGGGACACTTGCAGGGATTGGGTTTGTGTCTGCTGATGTTGGTCTTGATGCTGACAAATGCAATGTTCTTGTCAACATTGAGCAACAGAGCCCTGATATTGCTCAAGGAGTTCACGGTCACTTGAGCAAGAAGCCTGAGGAAATTGGAGCTGGTGATCAAGGTCACATGTTTGGCTATGCCACTGACGAAACACCTGAACTAATGCCACTCACACATCCTGATGGTAAGACCCAAGTTACCGTTGAATACAAGAATGATAATGGAGCCATGGTCCCTATTCGCGTGCACACAATCCTCATTTCAACTCAACACGACGAAACTGTCACAAATGAAAAGATTGCTCAAGATTTGAAAGAGCATGTTATTAAACCTGTCGTCCCAGCTAAATACCTTGACGACAAGACCATCTTCCATCTCAAACCTTCAGGTAGGTTTGTGATTGGTGGACCTCATGGAGATGCTGGGCTCACCGGAAGGAAGATCATTATTGATACCTATGGTGGTTGGGGTGCTCATGGTGGAGAGGTGCCTTCTCAGGAAAGGATCCAACCAAGGTGGACAGGAGTGGTGCTTACATTGTTAGGCAAGCTGCAAAAGTGTTGTCGCTTCAGGGCTTGCTCGTCGTTGTATTGTGCAGGTTTCTTATGCAATTGGAGTGCCAGAGCCACTTTCTGGCCTGGAATGATTGCTATCAACCTTGACCTCACAAGAGGTGGCAAATTCAGGTACCAGAAGACTGCTGCTTACGGGCATTTTGGACGTGACGATCCTGATTTTACATGGGAGACTGTGAAGATGCTCAAGCCCAAGGCTTGA
Protein sequence

>MsG0180003497.01.T01

MAVKVLAAEKLSLLAMNPANNCYLFLFLFNVKDVKSHVLKRQTPQSRTRWECRVQTMADYTITGSAYMDTFLFTSESVNEGHPDKICDQVSDAILDACLEQDKESRLPARPDGTLAGIGFVSADVGLDADKCNVLVNIEQQSPDIAQGVHGHLSKKPEEIGAGDQGHMFGYATDETPELMPLTHPDGKTQVTVEYKNDNGAMVPIRVHTILISTQHDETVTNEKIAQDLKEHVIKPVVPAKYLDDKTIFHLKPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGEVPSQERIQPRWTGVVLTLLGKLQKCCRFRACSSLYCAGFLCNWSARATFWPGMIAINLDLTRGGKFRYQKTAAYGHFGRDDPDFTWETVKMLKPKA*