AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022923.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022923.01.T01 MTR_8g079990 93.443 183 12 0 1 183 1 183 3.04e-111 332
MsG0480022923.01.T02 MTR_8g079990 74.163 418 18 3 24 351 218 635 6.80e-176 506
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022923.01.T01 AT1G67680 53.299 197 76 3 1 183 1 195 2.48e-61 201
MsG0480022923.01.T01 AT1G67650 48.947 190 85 3 1 183 1 185 2.03e-52 177
MsG0480022923.01.T02 AT1G67680 52.184 412 103 4 26 347 234 641 3.92e-113 345
MsG0480022923.01.T02 AT1G67650 47.700 413 120 5 24 346 220 626 4.98e-110 337

Find 198 sgRNAs with CRISPR-Local

Find 282 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCTTGGGAAGCCACCTTTC+TGG 0.144780 4:+78624630 None:intergenic
GAAACATCTTTGGACCTTTC+AGG 0.148742 4:+78625702 None:intergenic
AATCTCAAAACCATCAATTT+TGG 0.164599 4:-78624279 MsG0480022923.01.T01:three_prime_UTR
GGTGGAGGACCTGGGTTTGC+AGG 0.208255 4:+78624657 None:intergenic
TTTCGCATAAACGGCGATTC+TGG 0.217938 4:+78628304 None:intergenic
AAAACAAACCAATGAATTAA+TGG 0.243804 4:+78624112 None:intergenic
AGGATGGCTGGATTCATTTC+GGG 0.250936 4:-78626663 MsG0480022923.01.T01:CDS
GGGAAGCAATATATGCAAAT+AGG 0.256001 4:-78625584 MsG0480022923.01.T01:three_prime_UTR
GAATGAAGCTTTGGAGTCTT+TGG 0.268189 4:-78626861 MsG0480022923.01.T01:CDS
TCTGGATCTGGTGGAGGACC+TGG 0.280134 4:+78624648 None:intergenic
GACAGCTTGGAGCGTACTTC+TGG 0.283282 4:-78624801 MsG0480022923.01.T01:three_prime_UTR
GGCTGCGACTGCGTCGGTTT+GGG 0.291056 4:+78628199 None:intergenic
ATCATTGATTGGCAGCTTCT+TGG 0.293542 4:-78625821 MsG0480022923.01.T01:intron
AGCTCGTGTTGTTCTTCCTC+TGG 0.294117 4:+78624587 None:intergenic
AAGAAGTCTAGGAAGTAATA+AGG 0.294799 4:-78624388 MsG0480022923.01.T01:three_prime_UTR
AAGGATGGCTGGATTCATTT+CGG 0.297450 4:-78626664 MsG0480022923.01.T01:CDS
GTCTCCTATTGCTGTACAAT+TGG 0.300746 4:-78625933 MsG0480022923.01.T01:three_prime_UTR
AAATGATGAAACTATGCTTT+TGG 0.305228 4:-78626825 MsG0480022923.01.T01:CDS
GCTCAGGTTGCGGCGGCGGC+TGG 0.317288 4:-78625216 MsG0480022923.01.T01:three_prime_UTR
CAGACTATCAGAAACATCTT+TGG 0.317452 4:+78625692 None:intergenic
CTTTCCTTCGTCGTTCGTTT+CGG 0.317730 4:+78624740 None:intergenic
TTGAATTGAACCAAAATTGA+TGG 0.318140 4:+78624269 None:intergenic
GGAAGCCACCTTTCTGGATC+TGG 0.323233 4:+78624636 None:intergenic
GACTGCGTCGGTTTGGGCTT+CGG 0.340662 4:+78628205 None:intergenic
CCCAGGTTCTTTACTTAGCC+AGG 0.341545 4:-78625239 MsG0480022923.01.T01:three_prime_UTR
CTTCAATCGTTTCGCATAAA+CGG 0.346283 4:+78628295 None:intergenic
CATTAAGCAGTTAGAACAAT+TGG 0.350143 4:-78624139 MsG0480022923.01.T01:three_prime_UTR
ACACGAGCTGCTCAAGTTAG+AGG 0.350211 4:-78624573 MsG0480022923.01.T01:three_prime_UTR
TCATTGATTGGCAGCTTCTT+GGG 0.354425 4:-78625820 MsG0480022923.01.T01:intron
TTCGCATAAACGGCGATTCT+GGG 0.357343 4:+78628305 None:intergenic
TTGGTTTACCATTAATTCAT+TGG 0.357887 4:-78624120 MsG0480022923.01.T01:three_prime_UTR
AGCAACAAGCAGCTTTGAAT+TGG 0.358344 4:-78626636 MsG0480022923.01.T01:CDS
CAGGGCTCAGGTTGCGGCGG+CGG 0.362726 4:-78625220 MsG0480022923.01.T01:three_prime_UTR
AATTTCCAACATATCAATCT+TGG 0.370710 4:+78626733 None:intergenic
AGGCTGCGACTGCGTCGGTT+TGG 0.372921 4:+78628198 None:intergenic
GCCGGCATATGTTGAATATC+TGG 0.373744 4:+78625156 None:intergenic
GTTTGGGCTTCGGAGCCATT+AGG 0.374566 4:+78628215 None:intergenic
TTTCGGCAATTCAATCTTCT+CGG 0.374802 4:-78627906 MsG0480022923.01.T01:CDS
GAAATACTGGCACAGTTTGC+TGG 0.375065 4:-78625279 MsG0480022923.01.T01:three_prime_UTR
AAACAAGTTGAATGAAGCTT+TGG 0.382642 4:-78626870 MsG0480022923.01.T01:CDS
ATTGAACTCGCATAGTGTAT+AGG 0.382811 4:+78624299 None:intergenic
TTTGCAGGGTTAAACCCTTT+AGG 0.386343 4:+78624672 None:intergenic
TCGATTTGGTGATGCTGTTT+CGG 0.392201 4:-78627923 MsG0480022923.01.T01:CDS
AATACTGGTACAACACTATC+AGG 0.392818 4:+78625351 None:intergenic
CTTAGCCAGGGCTCAGGTTG+CGG 0.398955 4:-78625226 MsG0480022923.01.T01:three_prime_UTR
AGGGCAGCTTGCAGCAATAC+TGG 0.399705 4:+78625336 None:intergenic
CTGGATCTGGTGGAGGACCT+GGG 0.400436 4:+78624649 None:intergenic
TAGCCCAATTGTACAGCAAT+AGG 0.402117 4:+78625929 None:intergenic
TTTCCACAGCTGAAACCTAA+TGG 0.402997 4:-78628230 MsG0480022923.01.T01:exon
AAAGACCTTGCCTGGATTGA+AGG 0.409184 4:-78624835 MsG0480022923.01.T01:three_prime_UTR
CCTAGACTTCTTTCTAGACT+TGG 0.410339 4:+78624399 None:intergenic
GGAAGCAATATATGCAAATA+GGG 0.413110 4:-78625583 MsG0480022923.01.T01:three_prime_UTR
TTGAACTCGCATAGTGTATA+GGG 0.415210 4:+78624300 None:intergenic
TGAAACTTTCAGCAAAAGAA+AGG 0.416899 4:-78625605 MsG0480022923.01.T01:three_prime_UTR
AAACATCTTTGGACCTTTCA+GGG 0.418525 4:+78625703 None:intergenic
ATCTGTAAGTTTAACGGCAT+TGG 0.418962 4:+78628108 None:intergenic
GAAGCTTCTGGTAGATATCC+AGG 0.423941 4:+78626762 None:intergenic
CGCCGCCGCAACCTGAGCCC+TGG 0.424323 4:+78625221 None:intergenic
TGCACTCTGAGTAGCACCTT+TGG 0.425319 4:+78624459 None:intergenic
TGGAAGGGCTGAGGAAATAC+TGG 0.426397 4:-78625292 MsG0480022923.01.T01:three_prime_UTR
GGACATGATTAAACGAGATA+TGG 0.428299 4:-78625768 MsG0480022923.01.T01:three_prime_UTR
TCTCCTATTGCTGTACAATT+GGG 0.428740 4:-78625932 MsG0480022923.01.T01:three_prime_UTR
AAAGTCCAAGATTGATATGT+TGG 0.431327 4:-78626738 MsG0480022923.01.T01:CDS
ATTGCTGCAAGCTGCCCTCT+TGG 0.433939 4:-78625331 MsG0480022923.01.T01:three_prime_UTR
TGAGGAGCTCGTTAAAAGTC+AGG 0.440388 4:-78624943 MsG0480022923.01.T01:three_prime_UTR
TGGTATCTTTGTTAGAGAAT+CGG 0.447976 4:+78625176 None:intergenic
CTGGAAGTTGATTGGTGGAT+TGG 0.450053 4:+78624494 None:intergenic
ACTCTGATCTGTAAGTTTAA+CGG 0.452703 4:+78628102 None:intergenic
CCTGGGAGGTTATACATGAA+TGG 0.453891 4:-78626046 MsG0480022923.01.T01:intron
TGCTGCCTTAGTTTGTATGC+AGG 0.454368 4:-78626702 MsG0480022923.01.T01:CDS
CAAAGCTTTCGCCTAGCTCA+TGG 0.456121 4:-78625636 MsG0480022923.01.T01:three_prime_UTR
TGCTGTAGTTAGAGATAAGC+AGG 0.456566 4:-78624541 MsG0480022923.01.T01:three_prime_UTR
CAGGTGTTAATGTGCGTGTT+TGG 0.457889 4:-78624219 MsG0480022923.01.T01:three_prime_UTR
AGGCTGCCTCATTCAAGTTA+AGG 0.458642 4:-78624999 MsG0480022923.01.T01:three_prime_UTR
CTGCTTGTTCTTTAATTCAA+AGG 0.458725 4:-78626605 MsG0480022923.01.T01:exon
GTGGAGGACCTGGGTTTGCA+GGG 0.460700 4:+78624658 None:intergenic
TCTAGACTTGGATGAAGGCT+TGG 0.461224 4:+78624411 None:intergenic
TTGATCAAAGATGATCGATT+TGG 0.465225 4:-78627937 MsG0480022923.01.T01:CDS
AAAAGTTGACCTCTCTCGCT+TGG 0.466137 4:+78624614 None:intergenic
TTCTTTCTAGACTTGGATGA+AGG 0.466315 4:+78624406 None:intergenic
AATAATCTTGTTTCCCTGAA+AGG 0.467300 4:-78625716 MsG0480022923.01.T01:three_prime_UTR
AAGAAACTAAAGACCTTGCC+TGG 0.467453 4:-78624843 MsG0480022923.01.T01:three_prime_UTR
ATCTGAAGTTCAAGGATGGC+TGG 0.467862 4:-78626675 MsG0480022923.01.T01:CDS
TTGGATGAAGGCTTGGATGA+AGG 0.468551 4:+78624418 None:intergenic
GCTCGTGTTGTTCTTCCTCT+GGG 0.470898 4:+78624588 None:intergenic
CTTTCGCCTAGCTCATGGAT+TGG 0.471476 4:-78625631 MsG0480022923.01.T01:three_prime_UTR
ATTGTTCTAACTGCTTAATG+AGG 0.476672 4:+78624142 None:intergenic
TGCTCAAGTTAGAGGCTCAC+AGG 0.477514 4:-78624565 MsG0480022923.01.T01:three_prime_UTR
GAAAGCCAAGGTATCCTAAA+GGG 0.478236 4:-78624686 MsG0480022923.01.T01:three_prime_UTR
TAAAGAACCTGGGACTTCTC+AGG 0.480535 4:+78625249 None:intergenic
ACAATTGGGCTATGTCCAAC+AGG 0.482796 4:-78625918 MsG0480022923.01.T01:intron
GTTGCTGCTTTCATTACTGA+GGG 0.484165 4:-78624324 MsG0480022923.01.T01:three_prime_UTR
GAAGCATAGAAGCGCTAGTT+GGG 0.486962 4:-78624920 MsG0480022923.01.T01:three_prime_UTR
TCTTTACTTAGCCAGGGCTC+AGG 0.490874 4:-78625232 MsG0480022923.01.T01:three_prime_UTR
TCAGGCCAGTATCTTCCATC+AGG 0.490990 4:+78625977 None:intergenic
AAAGTTGACCTCTCTCGCTT+GGG 0.493251 4:+78624615 None:intergenic
AGCCACCTTTCTGGATCTGG+TGG 0.495160 4:+78624639 None:intergenic
TCTGGAGTTAAACAAGTTGA+GGG 0.499079 4:-78624783 MsG0480022923.01.T01:three_prime_UTR
GAAACTTTCAGCAAAAGAAA+GGG 0.499900 4:-78625604 MsG0480022923.01.T01:three_prime_UTR
TCTTCGAGTGGAGGTGGAGC+GGG 0.502009 4:+78628175 None:intergenic
TGAAGGCTTGGATGAAGGCT+TGG 0.502378 4:+78624423 None:intergenic
TTCTGGAGTTAAACAAGTTG+AGG 0.502597 4:-78624784 MsG0480022923.01.T01:three_prime_UTR
TGATCCCTGCATACAAACTA+AGG 0.504267 4:+78626697 None:intergenic
CCAAGTCTAGAAAGAAGTCT+AGG 0.505506 4:-78624399 MsG0480022923.01.T01:three_prime_UTR
CTCAGCAACAACCACTGTTA+CGG 0.506217 4:-78628266 MsG0480022923.01.T01:five_prime_UTR
TTAAACCCTTTAGGATACCT+TGG 0.508195 4:+78624681 None:intergenic
CACCTTTCTGGATCTGGTGG+AGG 0.508999 4:+78624642 None:intergenic
GAGAGCCTGATGGAAGATAC+TGG 0.510907 4:-78625982 MsG0480022923.01.T01:three_prime_UTR
CGTAACAGTGGTTGTTGCTG+AGG 0.511235 4:+78628267 None:intergenic
AGATGCTATTGAAGCTTATG+TGG 0.514106 4:-78625789 MsG0480022923.01.T01:three_prime_UTR
CATTGGTGAATGCGGAAGCT+TGG 0.514363 4:+78628125 None:intergenic
AATAGATCTTCGAGTGGAGG+TGG 0.517073 4:+78628169 None:intergenic
GGGAATTGATGTGGACAGCT+TGG 0.519634 4:-78624814 MsG0480022923.01.T01:three_prime_UTR
ACACTATCAGGAAACATGTC+TGG 0.519719 4:+78625363 None:intergenic
CCAGGTTCTTTACTTAGCCA+GGG 0.523348 4:-78625238 MsG0480022923.01.T01:three_prime_UTR
TGTTGCTGCTTTCATTACTG+AGG 0.523354 4:-78624325 MsG0480022923.01.T01:three_prime_UTR
GGAAGCATAGAAGCGCTAGT+TGG 0.523436 4:-78624921 MsG0480022923.01.T01:three_prime_UTR
ACAAGGGTGGCAACAGTAGC+CGG 0.524474 4:+78625138 None:intergenic
TGCGACTGGAAGTTGATTGG+TGG 0.525449 4:+78624489 None:intergenic
AGCTGCACCCTCAACGTCGT+TGG 0.527024 4:+78625098 None:intergenic
ATCTTCTCGGTCTCCTCCTG+AGG 0.529120 4:-78627893 MsG0480022923.01.T01:CDS
GAACGAGCCAACGACGTTGA+GGG 0.529678 4:-78625105 MsG0480022923.01.T01:three_prime_UTR
AGAGAATCGGCTGCAATATG+TGG 0.533632 4:+78625189 None:intergenic
ACCAGATATTCAACATATGC+CGG 0.534672 4:-78625157 MsG0480022923.01.T01:three_prime_UTR
AGAAAGCCAAGGTATCCTAA+AGG 0.536400 4:-78624687 MsG0480022923.01.T01:three_prime_UTR
TTGGATGTGTTTGTTGAGAG+TGG 0.536512 4:+78628144 None:intergenic
GCTGCCTTAGTTTGTATGCA+GGG 0.536618 4:-78626701 MsG0480022923.01.T01:CDS
ACATCAATTCCCTTCAATCC+AGG 0.536875 4:+78624825 None:intergenic
TCCATGCCTTAACTTGAATG+AGG 0.536971 4:+78624993 None:intergenic
GTTAGAGATAAGCAGGAAGC+TGG 0.537836 4:-78624534 MsG0480022923.01.T01:three_prime_UTR
TTGGTTAGAGAGAACAAAGC+TGG 0.538133 4:-78625312 MsG0480022923.01.T01:three_prime_UTR
CATTCAAGTTAAGGCATGGA+AGG 0.544217 4:-78624990 MsG0480022923.01.T01:three_prime_UTR
CCCTGGCTAAGTAAAGAACC+TGG 0.544789 4:+78625238 None:intergenic
AACTGCAATGGACGATTCAT+CGG 0.545482 4:+78625745 None:intergenic
GCAGTTCTCGCAATTGCACT+TGG 0.545659 4:-78627994 MsG0480022923.01.T01:intron
AGAACGAGCCAACGACGTTG+AGG 0.546494 4:-78625106 MsG0480022923.01.T01:three_prime_UTR
AGCTCTTGTTGTCCGCCCGA+AGG 0.549212 4:-78626560 MsG0480022923.01.T01:intron
CTCTCTAATAACATCAAGAC+GGG 0.552640 4:+78624880 None:intergenic
CAGGGATCATCTGAAGTTCA+AGG 0.553022 4:-78626683 MsG0480022923.01.T01:CDS
CCTTTGGAGGAAGTTGCGAC+TGG 0.553568 4:+78624475 None:intergenic
GCGGGAGGCTGCGACTGCGT+CGG 0.553576 4:+78628193 None:intergenic
AGTTGCGACTGGAAGTTGAT+TGG 0.553730 4:+78624486 None:intergenic
GCCTCATTCAAGTTAAGGCA+TGG 0.556079 4:-78624994 MsG0480022923.01.T01:three_prime_UTR
AAGACCTTGCCTGGATTGAA+GGG 0.556211 4:-78624834 MsG0480022923.01.T01:three_prime_UTR
TTGTTCTAACTGCTTAATGA+GGG 0.556776 4:+78624143 None:intergenic
GTAACAGTGGTTGTTGCTGA+GGG 0.557553 4:+78628268 None:intergenic
ATGCTGCTCTAAACTCTATG+AGG 0.559552 4:-78624961 MsG0480022923.01.T01:three_prime_UTR
TCGCATAAACGGCGATTCTG+GGG 0.561772 4:+78628306 None:intergenic
ACTCTGAGTAGCACCTTTGG+AGG 0.562248 4:+78624462 None:intergenic
TCAAGTCCAATCCATGAGCT+AGG 0.562856 4:+78625625 None:intergenic
GAACAAAGCTGGAAGGGCTG+AGG 0.564078 4:-78625301 MsG0480022923.01.T01:three_prime_UTR
TCGAGTGGAGGTGGAGCGGG+AGG 0.571792 4:+78628178 None:intergenic
ATTATTGACTGCAACTGCAA+TGG 0.572155 4:+78625733 None:intergenic
GATTGGTGGATTGGAATGAC+TGG 0.573915 4:+78624503 None:intergenic
ATAGTCTGAAGAAACTTGAT+CGG 0.576762 4:-78625677 MsG0480022923.01.T01:three_prime_UTR
GTTGAGGGTCCTCTTCATGC+AGG 0.576924 4:-78624768 MsG0480022923.01.T01:three_prime_UTR
GTCCTCCACCAGATCCAGAA+AGG 0.583333 4:-78624644 MsG0480022923.01.T01:three_prime_UTR
GCTTAATATTATAATGCAAG+AGG 0.584885 4:-78625019 MsG0480022923.01.T01:three_prime_UTR
GGTGGCTTCCCAAGCGAGAG+AGG 0.586065 4:-78624623 MsG0480022923.01.T01:three_prime_UTR
TAGAGAGAACAAAGCTGGAA+GGG 0.591318 4:-78625307 MsG0480022923.01.T01:three_prime_UTR
TTGATTCTGCAACCAAGTGG+TGG 0.591734 4:-78625068 MsG0480022923.01.T01:three_prime_UTR
ATCTTCGAGTGGAGGTGGAG+CGG 0.593310 4:+78628174 None:intergenic
GTATATTTGTCTATTGATGC+AGG 0.594079 4:-78624238 MsG0480022923.01.T01:three_prime_UTR
CCAGTCGCAACTTCCTCCAA+AGG 0.596576 4:-78624475 MsG0480022923.01.T01:three_prime_UTR
AAAGTTGCCTGAGAAGTCCC+AGG 0.599008 4:-78625256 MsG0480022923.01.T01:three_prime_UTR
TTTAACGGCATTGGTGAATG+CGG 0.600801 4:+78628117 None:intergenic
GAGCCATTAGGTTTCAGCTG+TGG 0.603914 4:+78628227 None:intergenic
AGCCAGGGCTCAGGTTGCGG+CGG 0.605720 4:-78625223 MsG0480022923.01.T01:three_prime_UTR
GGGTTTAACCCTGCAAACCC+AGG 0.606815 4:-78624666 MsG0480022923.01.T01:three_prime_UTR
TTAGAGAGAACAAAGCTGGA+AGG 0.608924 4:-78625308 MsG0480022923.01.T01:three_prime_UTR
TCATGCTAACAGGATTGACC+AGG 0.612401 4:-78625547 MsG0480022923.01.T01:intron
GCTCTCTAATAACATCAAGA+CGG 0.617542 4:+78624879 None:intergenic
GAGAATCGGCTGCAATATGT+GGG 0.621190 4:+78625190 None:intergenic
CATAGCATTAGACCACCACT+TGG 0.621895 4:+78625056 None:intergenic
TCGGCAACACCTGCATGAAG+AGG 0.622400 4:+78624759 None:intergenic
AAAAGAGAAAGAGAAAGCCA+AGG 0.626179 4:-78624698 MsG0480022923.01.T01:three_prime_UTR
TTTGTTCTCTCTAACCAAGA+GGG 0.627104 4:+78625317 None:intergenic
TGTTCTAACTGCTTAATGAG+GGG 0.627140 4:+78624144 None:intergenic
TGCTTGATTCTGCAACCAAG+TGG 0.627939 4:-78625071 MsG0480022923.01.T01:three_prime_UTR
GATCATCTGAAGTTCAAGGA+TGG 0.631439 4:-78626679 MsG0480022923.01.T01:CDS
TGGATTGAAGGGAATTGATG+TGG 0.636123 4:-78624823 MsG0480022923.01.T01:three_prime_UTR
ATTCAAGTTAAGGCATGGAA+GGG 0.638452 4:-78624989 MsG0480022923.01.T01:three_prime_UTR
GCTCAAGTTAGAGGCTCACA+GGG 0.642165 4:-78624564 MsG0480022923.01.T01:three_prime_UTR
GTTGGGCTGATAACAACAGT+TGG 0.643489 4:-78624903 MsG0480022923.01.T01:three_prime_UTR
GAGGAGCTCGTTAAAAGTCA+GGG 0.645289 4:-78624942 MsG0480022923.01.T01:three_prime_UTR
GTGAATAGATCTTCGAGTGG+AGG 0.646165 4:+78628166 None:intergenic
CCTGGCTAAGTAAAGAACCT+GGG 0.646230 4:+78625239 None:intergenic
AATTCCCTTCAATCCAGGCA+AGG 0.648744 4:+78624830 None:intergenic
CTCCACCAGATCCAGAAAGG+TGG 0.653977 4:-78624641 MsG0480022923.01.T01:three_prime_UTR
CTTTGTTCTCTCTAACCAAG+AGG 0.654169 4:+78625316 None:intergenic
AATCAGTATCACCGTAACAG+TGG 0.667307 4:+78628255 None:intergenic
GTGGTCTAATGCTATGACTG+AGG 0.668030 4:-78625049 MsG0480022923.01.T01:three_prime_UTR
TCAACGCAACGATCTTGCAT+CGG 0.668802 4:+78627956 None:intergenic
GAATTGGTCAGAGAGCCTGA+TGG 0.683025 4:-78625992 MsG0480022923.01.T01:three_prime_UTR
AATCGGCTGCAATATGTGGG+TGG 0.683879 4:+78625193 None:intergenic
GTGGTGAATAGATCTTCGAG+TGG 0.684779 4:+78628163 None:intergenic
GTTGCCGAAACGAACGACGA+AGG 0.686534 4:-78624744 MsG0480022923.01.T01:three_prime_UTR
AATATCACATCACCTTCGGG+CGG 0.714098 4:+78626548 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTATTATTATTAATTATA+TGG - Chr4:78626989-78627008 MsG0480022923.01.T01:intron 0.0%
!! ATATAAATCATTTGTATATA+CGG - Chr4:78625234-78625253 MsG0480022923.01.T01:three_prime_UTR 10.0%
!! TTGCTTATTATATTTATATA+AGG - Chr4:78624670-78624689 MsG0480022923.01.T01:three_prime_UTR 10.0%
!! AATGGTTAATATTATAAACA+TGG + Chr4:78625295-78625314 None:intergenic 15.0%
!! ATATTATATATGTACATACA+GGG + Chr4:78625401-78625420 None:intergenic 15.0%
!! ATCAATGAATTGAAATTAAA+AGG + Chr4:78624361-78624380 None:intergenic 15.0%
!! GAAATAATTTATGACTTAAT+AGG + Chr4:78626352-78626371 None:intergenic 15.0%
!!! AAAATATCTGAAGTAAATTT+GGG + Chr4:78625918-78625937 None:intergenic 15.0%
!!! AATTTAAAAGTTTTAACCAA+CGG - Chr4:78624764-78624783 MsG0480022923.01.T01:three_prime_UTR 15.0%
!!! TATATTTTTTCTATCAGAAT+TGG - Chr4:78626409-78626428 MsG0480022923.01.T01:intron 15.0%
!!! TTACTTTGTTGTTTTTAAAT+AGG - Chr4:78625505-78625524 MsG0480022923.01.T01:intron 15.0%
!! AAAACAAACCAATGAATTAA+TGG + Chr4:78628308-78628327 None:intergenic 20.0%
!! AATATAGTTTATGACAAAAC+AGG + Chr4:78624807-78624826 None:intergenic 20.0%
!! ACAAAGTAAAAAATAATCGA+TGG + Chr4:78625494-78625513 None:intergenic 20.0%
!! ATTGAATATTTGAATAGTGT+TGG + Chr4:78625991-78626010 None:intergenic 20.0%
!! CATATTATATATGTACATAC+AGG + Chr4:78625402-78625421 None:intergenic 20.0%
!! TAAGTCATAAATTATTTCCT+GGG - Chr4:78626354-78626373 MsG0480022923.01.T01:intron 20.0%
!! TTAAAAAAATGAAAATCCTC+AGG + Chr4:78624543-78624562 None:intergenic 20.0%
!! TTAAGTCATAAATTATTTCC+TGG - Chr4:78626353-78626372 MsG0480022923.01.T01:intron 20.0%
!! TTATTCATCGAATTATGAAA+TGG - Chr4:78624962-78624981 MsG0480022923.01.T01:three_prime_UTR 20.0%
!!! ACAATGTAACATTTTTTACA+CGG + Chr4:78625083-78625102 None:intergenic 20.0%
!!! AGTTTTAATCAATCATTGAT+TGG - Chr4:78626585-78626604 MsG0480022923.01.T01:three_prime_UTR 20.0%
!!! ATTTACTTCAGATATTTTCA+TGG - Chr4:78625920-78625939 MsG0480022923.01.T01:three_prime_UTR 20.0%
!!! GAAAATATCTGAAGTAAATT+TGG + Chr4:78625919-78625938 None:intergenic 20.0%
!!! TGAGGATTTTCATTTTTTTA+AGG - Chr4:78624542-78624561 MsG0480022923.01.T01:three_prime_UTR 20.0%
!!! TTTACTTCAGATATTTTCAT+GGG - Chr4:78625921-78625940 MsG0480022923.01.T01:three_prime_UTR 20.0%
! AAGTTCCTTATTGTAAAACA+TGG - Chr4:78626260-78626279 MsG0480022923.01.T01:intron 25.0%
! AATTTCCAACATATCAATCT+TGG + Chr4:78625687-78625706 None:intergenic 25.0%
! GATATATGACTGATGATTTA+AGG + Chr4:78626916-78626935 None:intergenic 25.0%
! GCTTAATATTATAATGCAAG+AGG - Chr4:78627398-78627417 MsG0480022923.01.T01:intron 25.0%
! TTGAATTGAACCAAAATTGA+TGG + Chr4:78628151-78628170 None:intergenic 25.0%
! TTGGTTTACCATTAATTCAT+TGG - Chr4:78628297-78628316 MsG0480022923.01.T01:five_prime_UTR 25.0%
!! AAATGATGAAACTATGCTTT+TGG - Chr4:78625592-78625611 MsG0480022923.01.T01:three_prime_UTR 25.0%
!! AATCTCAAAACCATCAATTT+TGG - Chr4:78628138-78628157 MsG0480022923.01.T01:CDS 25.0%
!! CAAAATAAGCCTTATACAAA+CGG - Chr4:78624888-78624907 MsG0480022923.01.T01:three_prime_UTR 25.0%
!! TACAATCAAATGTTGTTTGT+CGG - Chr4:78626299-78626318 MsG0480022923.01.T01:intron 25.0%
!! TCATTTTCTATATCTTCATC+AGG + Chr4:78626461-78626480 None:intergenic 25.0%
!! TTTGTCCATGTTTTACAATA+AGG + Chr4:78626268-78626287 None:intergenic 25.0%
!!! AAGCTCTAAAATAAGTCAAT+CGG + Chr4:78624702-78624721 None:intergenic 25.0%
AAAAAATGAAAATCCTCAGG+AGG + Chr4:78624540-78624559 None:intergenic 30.0%
AAAGTCCAAGATTGATATGT+TGG - Chr4:78625679-78625698 MsG0480022923.01.T01:three_prime_UTR 30.0%
AAGAAGTCTAGGAAGTAATA+AGG - Chr4:78628029-78628048 MsG0480022923.01.T01:intron 30.0%
ACAAAATTAGTTCATGCACA+TGG + Chr4:78625034-78625053 None:intergenic 30.0%
ACTCTGATCTGTAAGTTTAA+CGG + Chr4:78624318-78624337 None:intergenic 30.0%
ATAGTCTGAAGAAACTTGAT+CGG - Chr4:78626740-78626759 MsG0480022923.01.T01:CDS 30.0%
ATCAAATATCACATCACCTT+CGG + Chr4:78625876-78625895 None:intergenic 30.0%
ATCATTTGTATATACGGAAG+TGG - Chr4:78625240-78625259 MsG0480022923.01.T01:three_prime_UTR 30.0%
ATTGTTCTAACTGCTTAATG+AGG + Chr4:78628278-78628297 None:intergenic 30.0%
CATGTCATAGAAAACTTTGT+TGG - Chr4:78625439-78625458 MsG0480022923.01.T01:intron 30.0%
CATTAAGCAGTTAGAACAAT+TGG - Chr4:78628278-78628297 MsG0480022923.01.T01:five_prime_UTR 30.0%
CTAAAATAAGTCAATCGGAA+CGG + Chr4:78624697-78624716 None:intergenic 30.0%
CTGCTTGTTCTTTAATTCAA+AGG - Chr4:78625812-78625831 MsG0480022923.01.T01:intron 30.0%
GAAAACTTTGTTGGAGTTTA+TGG - Chr4:78625448-78625467 MsG0480022923.01.T01:intron 30.0%
GAAACTTTCAGCAAAAGAAA+GGG - Chr4:78626813-78626832 MsG0480022923.01.T01:CDS 30.0%
GAACTCTAGTAGTAGATATT+AGG - Chr4:78625007-78625026 MsG0480022923.01.T01:three_prime_UTR 30.0%
GGAAGCAATATATGCAAATA+GGG - Chr4:78626834-78626853 MsG0480022923.01.T01:CDS 30.0%
GTATATTTGTCTATTGATGC+AGG - Chr4:78628179-78628198 MsG0480022923.01.T01:CDS 30.0%
GTGATGTGATATTTGATTGT+GGG - Chr4:78625879-78625898 MsG0480022923.01.T01:intron 30.0%
GTGTTAAAAAATAGACATCG+AGG + Chr4:78625322-78625341 None:intergenic 30.0%
TAAAATAAGTCAATCGGAAC+GGG + Chr4:78624696-78624715 None:intergenic 30.0%
TGAAACTTTCAGCAAAAGAA+AGG - Chr4:78626812-78626831 MsG0480022923.01.T01:CDS 30.0%
TGAAATTCAGAATTAGAGCT+AGG - Chr4:78624612-78624631 MsG0480022923.01.T01:three_prime_UTR 30.0%
TGGTATCTTTGTTAGAGAAT+CGG + Chr4:78627244-78627263 None:intergenic 30.0%
TTGATCAAAGATGATCGATT+TGG - Chr4:78624480-78624499 MsG0480022923.01.T01:three_prime_UTR 30.0%
TTGTTCTAACTGCTTAATGA+GGG + Chr4:78628277-78628296 None:intergenic 30.0%
TTTGTATCGTCTTGGAAAAA+TGG - Chr4:78625625-78625644 MsG0480022923.01.T01:three_prime_UTR 30.0%
! AAACAAGTTGAATGAAGCTT+TGG - Chr4:78625547-78625566 MsG0480022923.01.T01:intron 30.0%
! AATAATCTTGTTTCCCTGAA+AGG - Chr4:78626701-78626720 MsG0480022923.01.T01:CDS 30.0%
! AATTATGAAATGGAAGCATG+GGG - Chr4:78624972-78624991 MsG0480022923.01.T01:three_prime_UTR 30.0%
! GAATTATGAAATGGAAGCAT+GGG - Chr4:78624971-78624990 MsG0480022923.01.T01:three_prime_UTR 30.0%
! TCTCAAATTTTGTATCGTCT+TGG - Chr4:78625617-78625636 MsG0480022923.01.T01:three_prime_UTR 30.0%
!! AATTTTGGTCATGCATCTTT+TGG + Chr4:78625359-78625378 None:intergenic 30.0%
!! TCGTTCTTTTAAAGACACAA+GGG + Chr4:78627298-78627317 None:intergenic 30.0%
AAAAGAGAAAGAGAAAGCCA+AGG - Chr4:78627719-78627738 MsG0480022923.01.T01:intron 35.0%
AAACACATACTCGTGAAGTT+TGG + Chr4:78626115-78626134 None:intergenic 35.0%
AAACATCTTTGGACCTTTCA+GGG + Chr4:78626717-78626736 None:intergenic 35.0%
AAGAAGACAGATAAAAGACC+TGG + Chr4:78626891-78626910 None:intergenic 35.0%
AATACTGGTACAACACTATC+AGG + Chr4:78627069-78627088 None:intergenic 35.0%
AATAGACATCGAGGATTGAA+TGG + Chr4:78625313-78625332 None:intergenic 35.0%
ACCAGATATTCAACATATGC+CGG - Chr4:78627260-78627279 MsG0480022923.01.T01:intron 35.0%
AGAGAGAAGGTGATAGTTTA+GGG - Chr4:78624925-78624944 MsG0480022923.01.T01:three_prime_UTR 35.0%
AGATGCTATTGAAGCTTATG+TGG - Chr4:78626628-78626647 MsG0480022923.01.T01:CDS 35.0%
ATCTGTAAGTTTAACGGCAT+TGG + Chr4:78624312-78624331 None:intergenic 35.0%
ATGACCAAAATTGAACATGG+GGG - Chr4:78625367-78625386 MsG0480022923.01.T01:three_prime_UTR 35.0%
ATTGAACTCGCATAGTGTAT+AGG + Chr4:78628121-78628140 None:intergenic 35.0%
CAGACTATCAGAAACATCTT+TGG + Chr4:78626728-78626747 None:intergenic 35.0%
CATGACCAAAATTGAACATG+GGG - Chr4:78625366-78625385 MsG0480022923.01.T01:three_prime_UTR 35.0%
CGAATTATGAAATGGAAGCA+TGG - Chr4:78624970-78624989 MsG0480022923.01.T01:three_prime_UTR 35.0%
CTCTCTAATAACATCAAGAC+GGG + Chr4:78627540-78627559 None:intergenic 35.0%
CTTCAATCGTTTCGCATAAA+CGG + Chr4:78624125-78624144 None:intergenic 35.0%
GCATGACCAAAATTGAACAT+GGG - Chr4:78625365-78625384 MsG0480022923.01.T01:three_prime_UTR 35.0%
GCTCTCTAATAACATCAAGA+CGG + Chr4:78627541-78627560 None:intergenic 35.0%
GGACATGATTAAACGAGATA+TGG - Chr4:78626649-78626668 MsG0480022923.01.T01:CDS 35.0%
GGGAAGCAATATATGCAAAT+AGG - Chr4:78626833-78626852 MsG0480022923.01.T01:CDS 35.0%
GGTGATGTGATATTTGATTG+TGG - Chr4:78625878-78625897 MsG0480022923.01.T01:intron 35.0%
GTCATAAATTATTTCCTGGG+AGG - Chr4:78626357-78626376 MsG0480022923.01.T01:intron 35.0%
TACAAACGGTTGGACTAAAT+TGG - Chr4:78624902-78624921 MsG0480022923.01.T01:three_prime_UTR 35.0%
TACGAAGATACAATTTCCGT+TGG + Chr4:78624783-78624802 None:intergenic 35.0%
TCAAATATCACATCACCTTC+GGG + Chr4:78625875-78625894 None:intergenic 35.0%
TCAGAATTAGAGCTAGGATA+GGG - Chr4:78624618-78624637 MsG0480022923.01.T01:three_prime_UTR 35.0%
TCTCCTATTGCTGTACAATT+GGG - Chr4:78626485-78626504 MsG0480022923.01.T01:intron 35.0%
TGCATGACCAAAATTGAACA+TGG - Chr4:78625364-78625383 MsG0480022923.01.T01:three_prime_UTR 35.0%
TGTTCTAACTGCTTAATGAG+GGG + Chr4:78628276-78628295 None:intergenic 35.0%
TTAGTCCAACCGTTTGTATA+AGG + Chr4:78624900-78624919 None:intergenic 35.0%
TTCAGAATTAGAGCTAGGAT+AGG - Chr4:78624617-78624636 MsG0480022923.01.T01:three_prime_UTR 35.0%
TTCTTTCTAGACTTGGATGA+AGG + Chr4:78628014-78628033 None:intergenic 35.0%
TTGAACTCGCATAGTGTATA+GGG + Chr4:78628120-78628139 None:intergenic 35.0%
TTTCGGCAATTCAATCTTCT+CGG - Chr4:78624511-78624530 MsG0480022923.01.T01:three_prime_UTR 35.0%
TTTGTTCTCTCTAACCAAGA+GGG + Chr4:78627103-78627122 None:intergenic 35.0%
! AATTCCCCCATGTTCAATTT+TGG + Chr4:78625374-78625393 None:intergenic 35.0%
! ATAAGCCTTATACAAACGGT+TGG - Chr4:78624892-78624911 MsG0480022923.01.T01:three_prime_UTR 35.0%
! ATTATTGACTGCAACTGCAA+TGG + Chr4:78626687-78626706 None:intergenic 35.0%
! ATTCAAGTTAAGGCATGGAA+GGG - Chr4:78627428-78627447 MsG0480022923.01.T01:intron 35.0%
! CACGAAGCATCTATTACTAA+TGG + Chr4:78626209-78626228 None:intergenic 35.0%
! TCTGGAGTTAAACAAGTTGA+GGG - Chr4:78627634-78627653 MsG0480022923.01.T01:intron 35.0%
! TTTTGCTTCTTCATGCTAAC+AGG - Chr4:78626860-78626879 MsG0480022923.01.T01:CDS 35.0%
!! CTCGTTCTTTTAAAGACACA+AGG + Chr4:78627299-78627318 None:intergenic 35.0%
!! TTAAACCCTTTAGGATACCT+TGG + Chr4:78627739-78627758 None:intergenic 35.0%
!! TTCTGGAGTTAAACAAGTTG+AGG - Chr4:78627633-78627652 MsG0480022923.01.T01:intron 35.0%
!! TTCTTTTAAAGACACAAGGG+TGG + Chr4:78627295-78627314 None:intergenic 35.0%
AAATCAGAACTCCTACCTGT+TGG + Chr4:78626517-78626536 None:intergenic 40.0%
AACTGCAATGGACGATTCAT+CGG + Chr4:78626675-78626694 None:intergenic 40.0%
AAGAAACTAAAGACCTTGCC+TGG - Chr4:78627574-78627593 MsG0480022923.01.T01:intron 40.0%
AAGGATGGCTGGATTCATTT+CGG - Chr4:78625753-78625772 MsG0480022923.01.T01:three_prime_UTR 40.0%
AATCAGTATCACCGTAACAG+TGG + Chr4:78624165-78624184 None:intergenic 40.0%
ACACTATCAGGAAACATGTC+TGG + Chr4:78627057-78627076 None:intergenic 40.0%
ACATCAATTCCCTTCAATCC+AGG + Chr4:78627595-78627614 None:intergenic 40.0%
ATGGTTACATCGCAATCTGT+AGG - Chr4:78627008-78627027 MsG0480022923.01.T01:intron 40.0%
CCAAGTCTAGAAAGAAGTCT+AGG - Chr4:78628018-78628037 MsG0480022923.01.T01:intron 40.0%
CCTAGACTTCTTTCTAGACT+TGG + Chr4:78628021-78628040 None:intergenic 40.0%
CTTTGTTCTCTCTAACCAAG+AGG + Chr4:78627104-78627123 None:intergenic 40.0%
GAAACATCTTTGGACCTTTC+AGG + Chr4:78626718-78626737 None:intergenic 40.0%
GAGAGAGAAGGTGATAGTTT+AGG - Chr4:78624924-78624943 MsG0480022923.01.T01:three_prime_UTR 40.0%
GTCTCCTATTGCTGTACAAT+TGG - Chr4:78626484-78626503 MsG0480022923.01.T01:intron 40.0%
GTTTGCAAGTCAAGTGTGAT+TGG - Chr4:78626228-78626247 MsG0480022923.01.T01:intron 40.0%
TAGAGAGAACAAAGCTGGAA+GGG - Chr4:78627110-78627129 MsG0480022923.01.T01:intron 40.0%
TAGCCCAATTGTACAGCAAT+AGG + Chr4:78626491-78626510 None:intergenic 40.0%
TGATCCCTGCATACAAACTA+AGG + Chr4:78625723-78625742 None:intergenic 40.0%
TGCTGTAGTTAGAGATAAGC+AGG - Chr4:78627876-78627895 MsG0480022923.01.T01:CDS 40.0%
TGGACTAAATTGGAGAGAGA+AGG - Chr4:78624912-78624931 MsG0480022923.01.T01:three_prime_UTR 40.0%
TGGATTGAAGGGAATTGATG+TGG - Chr4:78627594-78627613 MsG0480022923.01.T01:intron 40.0%
TTAGAGAGAACAAAGCTGGA+AGG - Chr4:78627109-78627128 MsG0480022923.01.T01:intron 40.0%
TTGGTTAGAGAGAACAAAGC+TGG - Chr4:78627105-78627124 MsG0480022923.01.T01:intron 40.0%
TTTCCACAGCTGAAACCTAA+TGG - Chr4:78624187-78624206 MsG0480022923.01.T01:three_prime_UTR 40.0%
TTTGCAGGGTTAAACCCTTT+AGG + Chr4:78627748-78627767 None:intergenic 40.0%
! AGAAAGCCAAGGTATCCTAA+AGG - Chr4:78627730-78627749 MsG0480022923.01.T01:intron 40.0%
! ATCATTGATTGGCAGCTTCT+TGG - Chr4:78626596-78626615 MsG0480022923.01.T01:exon 40.0%
! ATGCTGCTCTAAACTCTATG+AGG - Chr4:78627456-78627475 MsG0480022923.01.T01:intron 40.0%
! CATTCAAGTTAAGGCATGGA+AGG - Chr4:78627427-78627446 MsG0480022923.01.T01:intron 40.0%
! GAAAGCCAAGGTATCCTAAA+GGG - Chr4:78627731-78627750 MsG0480022923.01.T01:intron 40.0%
! GATCATCTGAAGTTCAAGGA+TGG - Chr4:78625738-78625757 MsG0480022923.01.T01:three_prime_UTR 40.0%
! TCATTGATTGGCAGCTTCTT+GGG - Chr4:78626597-78626616 MsG0480022923.01.T01:exon 40.0%
! TCCATGCCTTAACTTGAATG+AGG + Chr4:78627427-78627446 None:intergenic 40.0%
! TTGGATGTGTTTGTTGAGAG+TGG + Chr4:78624276-78624295 None:intergenic 40.0%
!! AGCAACAAGCAGCTTTGAAT+TGG - Chr4:78625781-78625800 MsG0480022923.01.T01:three_prime_UTR 40.0%
!! GAATGAAGCTTTGGAGTCTT+TGG - Chr4:78625556-78625575 MsG0480022923.01.T01:three_prime_UTR 40.0%
!! GTTGCTGCTTTCATTACTGA+GGG - Chr4:78628093-78628112 MsG0480022923.01.T01:intron 40.0%
!! TCGATTTGGTGATGCTGTTT+CGG - Chr4:78624494-78624513 MsG0480022923.01.T01:three_prime_UTR 40.0%
!! TGTTGCTGCTTTCATTACTG+AGG - Chr4:78628092-78628111 MsG0480022923.01.T01:intron 40.0%
!! TTTAACGGCATTGGTGAATG+CGG + Chr4:78624303-78624322 None:intergenic 40.0%
!!! TCTTGGACTTTTGAAGCTTC+TGG + Chr4:78625670-78625689 None:intergenic 40.0%
AAAAGTTGACCTCTCTCGCT+TGG + Chr4:78627806-78627825 None:intergenic 45.0%
AAAGACCTTGCCTGGATTGA+AGG - Chr4:78627582-78627601 MsG0480022923.01.T01:intron 45.0%
AAAGTTGACCTCTCTCGCTT+GGG + Chr4:78627805-78627824 None:intergenic 45.0%
AAGACCTTGCCTGGATTGAA+GGG - Chr4:78627583-78627602 MsG0480022923.01.T01:intron 45.0%
AATAGATCTTCGAGTGGAGG+TGG + Chr4:78624251-78624270 None:intergenic 45.0%
AATATCACATCACCTTCGGG+CGG + Chr4:78625872-78625891 None:intergenic 45.0%
AATTCCCTTCAATCCAGGCA+AGG + Chr4:78627590-78627609 None:intergenic 45.0%
ACAATTGGGCTATGTCCAAC+AGG - Chr4:78626499-78626518 MsG0480022923.01.T01:intron 45.0%
AGAGAATCGGCTGCAATATG+TGG + Chr4:78627231-78627250 None:intergenic 45.0%
AGGATGGCTGGATTCATTTC+GGG - Chr4:78625754-78625773 MsG0480022923.01.T01:three_prime_UTR 45.0%
CAGGTGTTAATGTGCGTGTT+TGG - Chr4:78628198-78628217 MsG0480022923.01.T01:CDS 45.0%
CATAGCATTAGACCACCACT+TGG + Chr4:78627364-78627383 None:intergenic 45.0%
CCAGGTTCTTTACTTAGCCA+GGG - Chr4:78627179-78627198 MsG0480022923.01.T01:intron 45.0%
CCATTCATGTATAACCTCCC+AGG + Chr4:78626374-78626393 None:intergenic 45.0%
CCTGGCTAAGTAAAGAACCT+GGG + Chr4:78627181-78627200 None:intergenic 45.0%
CCTGGGAGGTTATACATGAA+TGG - Chr4:78626371-78626390 MsG0480022923.01.T01:intron 45.0%
CTCAGCAACAACCACTGTTA+CGG - Chr4:78624151-78624170 MsG0480022923.01.T01:three_prime_UTR 45.0%
CTTTCCTTCGTCGTTCGTTT+CGG + Chr4:78627680-78627699 None:intergenic 45.0%
GAAATACTGGCACAGTTTGC+TGG - Chr4:78627138-78627157 MsG0480022923.01.T01:intron 45.0%
GAAGCTTCTGGTAGATATCC+AGG + Chr4:78625658-78625677 None:intergenic 45.0%
GAGAATCGGCTGCAATATGT+GGG + Chr4:78627230-78627249 None:intergenic 45.0%
GAGGAGCTCGTTAAAAGTCA+GGG - Chr4:78627475-78627494 MsG0480022923.01.T01:intron 45.0%
GCCGGCATATGTTGAATATC+TGG + Chr4:78627264-78627283 None:intergenic 45.0%
GTAACAGTGGTTGTTGCTGA+GGG + Chr4:78624152-78624171 None:intergenic 45.0%
GTGAATAGATCTTCGAGTGG+AGG + Chr4:78624254-78624273 None:intergenic 45.0%
GTGGTCTAATGCTATGACTG+AGG - Chr4:78627368-78627387 MsG0480022923.01.T01:intron 45.0%
GTGGTGAATAGATCTTCGAG+TGG + Chr4:78624257-78624276 None:intergenic 45.0%
GTTAGAGATAAGCAGGAAGC+TGG - Chr4:78627883-78627902 MsG0480022923.01.T01:CDS 45.0%
GTTGGGCTGATAACAACAGT+TGG - Chr4:78627514-78627533 MsG0480022923.01.T01:intron 45.0%
TAAAGAACCTGGGACTTCTC+AGG + Chr4:78627171-78627190 None:intergenic 45.0%
TCAACGCAACGATCTTGCAT+CGG + Chr4:78624464-78624483 None:intergenic 45.0%
TCAAGTCCAATCCATGAGCT+AGG + Chr4:78626795-78626814 None:intergenic 45.0%
TCATGCTAACAGGATTGACC+AGG - Chr4:78626870-78626889 MsG0480022923.01.T01:CDS 45.0%
TCTAGACTTGGATGAAGGCT+TGG + Chr4:78628009-78628028 None:intergenic 45.0%
TGAGGAGCTCGTTAAAAGTC+AGG - Chr4:78627474-78627493 MsG0480022923.01.T01:intron 45.0%
TGCTTGATTCTGCAACCAAG+TGG - Chr4:78627346-78627365 MsG0480022923.01.T01:intron 45.0%
TGGAAAAATGGATGCTTGCC+TGG - Chr4:78625637-78625656 MsG0480022923.01.T01:three_prime_UTR 45.0%
TTCGCATAAACGGCGATTCT+GGG + Chr4:78624115-78624134 None:intergenic 45.0%
TTGATTCTGCAACCAAGTGG+TGG - Chr4:78627349-78627368 MsG0480022923.01.T01:intron 45.0%
TTGGATGAAGGCTTGGATGA+AGG + Chr4:78628002-78628021 None:intergenic 45.0%
TTTCGCATAAACGGCGATTC+TGG + Chr4:78624116-78624135 None:intergenic 45.0%
! AGCGAGAGAGGTCAACTTTT+AGG - Chr4:78627806-78627825 MsG0480022923.01.T01:intron 45.0%
! AGGCTGCCTCATTCAAGTTA+AGG - Chr4:78627418-78627437 MsG0480022923.01.T01:intron 45.0%
! AGTTGCGACTGGAAGTTGAT+TGG + Chr4:78627934-78627953 None:intergenic 45.0%
! ATCTGAAGTTCAAGGATGGC+TGG - Chr4:78625742-78625761 MsG0480022923.01.T01:three_prime_UTR 45.0%
! CAGGGATCATCTGAAGTTCA+AGG - Chr4:78625734-78625753 MsG0480022923.01.T01:three_prime_UTR 45.0%
! CTGGAAGTTGATTGGTGGAT+TGG + Chr4:78627926-78627945 None:intergenic 45.0%
! GCCTCATTCAAGTTAAGGCA+TGG - Chr4:78627423-78627442 MsG0480022923.01.T01:intron 45.0%
!! GAAGCATAGAAGCGCTAGTT+GGG - Chr4:78627497-78627516 MsG0480022923.01.T01:intron 45.0%
!! GATTGGTGGATTGGAATGAC+TGG + Chr4:78627917-78627936 None:intergenic 45.0%
!! GCTGCCTTAGTTTGTATGCA+GGG - Chr4:78625716-78625735 MsG0480022923.01.T01:three_prime_UTR 45.0%
!! TGCTGCCTTAGTTTGTATGC+AGG - Chr4:78625715-78625734 MsG0480022923.01.T01:three_prime_UTR 45.0%
AAAGTTGCCTGAGAAGTCCC+AGG - Chr4:78627161-78627180 MsG0480022923.01.T01:intron 50.0%
AATCGGCTGCAATATGTGGG+TGG + Chr4:78627227-78627246 None:intergenic 50.0%
ACACGAGCTGCTCAAGTTAG+AGG - Chr4:78627844-78627863 MsG0480022923.01.T01:intron 50.0%
ACTCTGAGTAGCACCTTTGG+AGG + Chr4:78627958-78627977 None:intergenic 50.0%
CAAAGCTTTCGCCTAGCTCA+TGG - Chr4:78626781-78626800 MsG0480022923.01.T01:CDS 50.0%
CATTGGTGAATGCGGAAGCT+TGG + Chr4:78624295-78624314 None:intergenic 50.0%
CCCAGGTTCTTTACTTAGCC+AGG - Chr4:78627178-78627197 MsG0480022923.01.T01:intron 50.0%
CCCTGGCTAAGTAAAGAACC+TGG + Chr4:78627182-78627201 None:intergenic 50.0%
CGTAACAGTGGTTGTTGCTG+AGG + Chr4:78624153-78624172 None:intergenic 50.0%
CTTTCGCCTAGCTCATGGAT+TGG - Chr4:78626786-78626805 MsG0480022923.01.T01:CDS 50.0%
GAATTGGTCAGAGAGCCTGA+TGG - Chr4:78626425-78626444 MsG0480022923.01.T01:intron 50.0%
GAGAGCCTGATGGAAGATAC+TGG - Chr4:78626435-78626454 MsG0480022923.01.T01:intron 50.0%
GAGCCATTAGGTTTCAGCTG+TGG + Chr4:78624193-78624212 None:intergenic 50.0%
GCAGTTCTCGCAATTGCACT+TGG - Chr4:78624423-78624442 MsG0480022923.01.T01:three_prime_UTR 50.0%
GCTCAAGTTAGAGGCTCACA+GGG - Chr4:78627853-78627872 MsG0480022923.01.T01:intron 50.0%
GGGAATTGATGTGGACAGCT+TGG - Chr4:78627603-78627622 MsG0480022923.01.T01:intron 50.0%
TCAGGCCAGTATCTTCCATC+AGG + Chr4:78626443-78626462 None:intergenic 50.0%
TCGCATAAACGGCGATTCTG+GGG + Chr4:78624114-78624133 None:intergenic 50.0%
TCTTTACTTAGCCAGGGCTC+AGG - Chr4:78627185-78627204 MsG0480022923.01.T01:intron 50.0%
TGAAGGCTTGGATGAAGGCT+TGG + Chr4:78627997-78628016 None:intergenic 50.0%
TGCACTCTGAGTAGCACCTT+TGG + Chr4:78627961-78627980 None:intergenic 50.0%
TGCTCAAGTTAGAGGCTCAC+AGG - Chr4:78627852-78627871 MsG0480022923.01.T01:intron 50.0%
TGGAAGGGCTGAGGAAATAC+TGG - Chr4:78627125-78627144 MsG0480022923.01.T01:intron 50.0%
TTGGGCTATGTCCAACAGGT+AGG - Chr4:78626503-78626522 MsG0480022923.01.T01:intron 50.0%
! AGCTCGTGTTGTTCTTCCTC+TGG + Chr4:78627833-78627852 None:intergenic 50.0%
! AGGTCAACTTTTAGGCCCAG+AGG - Chr4:78627814-78627833 MsG0480022923.01.T01:intron 50.0%
! GCTCGTGTTGTTCTTCCTCT+GGG + Chr4:78627832-78627851 None:intergenic 50.0%
! TGCGACTGGAAGTTGATTGG+TGG + Chr4:78627931-78627950 None:intergenic 50.0%
!! GGAAGCATAGAAGCGCTAGT+TGG - Chr4:78627496-78627515 MsG0480022923.01.T01:intron 50.0%
ACAAGGGTGGCAACAGTAGC+CGG + Chr4:78627282-78627301 None:intergenic 55.0%
AGAACGAGCCAACGACGTTG+AGG - Chr4:78627311-78627330 MsG0480022923.01.T01:intron 55.0%
AGCCACCTTTCTGGATCTGG+TGG + Chr4:78627781-78627800 None:intergenic 55.0%
AGGGCAGCTTGCAGCAATAC+TGG + Chr4:78627084-78627103 None:intergenic 55.0%
ATCTTCGAGTGGAGGTGGAG+CGG + Chr4:78624246-78624265 None:intergenic 55.0%
ATCTTCTCGGTCTCCTCCTG+AGG - Chr4:78624524-78624543 MsG0480022923.01.T01:three_prime_UTR 55.0%
ATTGCTGCAAGCTGCCCTCT+TGG - Chr4:78627086-78627105 MsG0480022923.01.T01:intron 55.0%
CACCTTTCTGGATCTGGTGG+AGG + Chr4:78627778-78627797 None:intergenic 55.0%
CCAGTCGCAACTTCCTCCAA+AGG - Chr4:78627942-78627961 MsG0480022923.01.T01:CDS 55.0%
CTCCACCAGATCCAGAAAGG+TGG - Chr4:78627776-78627795 MsG0480022923.01.T01:intron 55.0%
GAACAAAGCTGGAAGGGCTG+AGG - Chr4:78627116-78627135 MsG0480022923.01.T01:intron 55.0%
GAACGAGCCAACGACGTTGA+GGG - Chr4:78627312-78627331 MsG0480022923.01.T01:intron 55.0%
GACAGCTTGGAGCGTACTTC+TGG - Chr4:78627616-78627635 MsG0480022923.01.T01:intron 55.0%
GGAAGCCACCTTTCTGGATC+TGG + Chr4:78627784-78627803 None:intergenic 55.0%
GGGTTTAACCCTGCAAACCC+AGG - Chr4:78627751-78627770 MsG0480022923.01.T01:intron 55.0%
GTCCTCCACCAGATCCAGAA+AGG - Chr4:78627773-78627792 MsG0480022923.01.T01:intron 55.0%
GTTGAGGGTCCTCTTCATGC+AGG - Chr4:78627649-78627668 MsG0480022923.01.T01:intron 55.0%
GTTGCCGAAACGAACGACGA+AGG - Chr4:78627673-78627692 MsG0480022923.01.T01:intron 55.0%
GTTTGGGCTTCGGAGCCATT+AGG + Chr4:78624205-78624224 None:intergenic 55.0%
TCGGCAACACCTGCATGAAG+AGG + Chr4:78627661-78627680 None:intergenic 55.0%
! CCTTTGGAGGAAGTTGCGAC+TGG + Chr4:78627945-78627964 None:intergenic 55.0%
AGCTGCACCCTCAACGTCGT+TGG + Chr4:78627322-78627341 None:intergenic 60.0%
CGCTTGGGAAGCCACCTTTC+TGG + Chr4:78627790-78627809 None:intergenic 60.0%
CTGGATCTGGTGGAGGACCT+GGG + Chr4:78627771-78627790 None:intergenic 60.0%
GTGGAGGACCTGGGTTTGCA+GGG + Chr4:78627762-78627781 None:intergenic 60.0%
TCTGGATCTGGTGGAGGACC+TGG + Chr4:78627772-78627791 None:intergenic 60.0%
TCTTCGAGTGGAGGTGGAGC+GGG + Chr4:78624245-78624264 None:intergenic 60.0%
! AGCTCTTGTTGTCCGCCCGA+AGG - Chr4:78625857-78625876 MsG0480022923.01.T01:intron 60.0%
! CTTAGCCAGGGCTCAGGTTG+CGG - Chr4:78627191-78627210 MsG0480022923.01.T01:intron 60.0%
!! GACTGCGTCGGTTTGGGCTT+CGG + Chr4:78624215-78624234 None:intergenic 60.0%
GGTGGAGGACCTGGGTTTGC+AGG + Chr4:78627763-78627782 None:intergenic 65.0%
GGTGGCTTCCCAAGCGAGAG+AGG - Chr4:78627794-78627813 MsG0480022923.01.T01:intron 65.0%
!! AGGCTGCGACTGCGTCGGTT+TGG + Chr4:78624222-78624241 None:intergenic 65.0%
!! GGCTGCGACTGCGTCGGTTT+GGG + Chr4:78624221-78624240 None:intergenic 65.0%
TCGAGTGGAGGTGGAGCGGG+AGG + Chr4:78624242-78624261 None:intergenic 70.0%
! AGCCAGGGCTCAGGTTGCGG+CGG - Chr4:78627194-78627213 MsG0480022923.01.T01:intron 70.0%
GCGGGAGGCTGCGACTGCGT+CGG + Chr4:78624227-78624246 None:intergenic 75.0%
! CAGGGCTCAGGTTGCGGCGG+CGG - Chr4:78627197-78627216 MsG0480022923.01.T01:intron 75.0%
! CGCCGCCGCAACCTGAGCCC+TGG + Chr4:78627199-78627218 None:intergenic 80.0%
!! GCTCAGGTTGCGGCGGCGGC+TGG - Chr4:78627201-78627220 MsG0480022923.01.T01:intron 80.0%
Chromosome Type Strat End Strand Name
Chr4 gene 78624109 78628330 78624109 ID=MsG0480022923.01;Name=MsG0480022923.01
Chr4 mRNA 78624109 78628330 78624109 ID=MsG0480022923.01.T01;Parent=MsG0480022923.01;Name=MsG0480022923.01.T01;_AED=0.18;_eAED=0.18;_QI=97|1|1|1|0.6|0.66|6|1729|188
Chr4 exon 78624109 78625409 78624109 ID=MsG0480022923.01.T01:exon:5993;Parent=MsG0480022923.01.T01
Chr4 exon 78625548 78625828 78625548 ID=MsG0480022923.01.T01:exon:5992;Parent=MsG0480022923.01.T01
Chr4 exon 78625919 78626013 78625919 ID=MsG0480022923.01.T01:exon:5991;Parent=MsG0480022923.01.T01
Chr4 exon 78626561 78626912 78626561 ID=MsG0480022923.01.T01:exon:5990;Parent=MsG0480022923.01.T01
Chr4 exon 78627876 78628012 78627876 ID=MsG0480022923.01.T01:exon:5989;Parent=MsG0480022923.01.T01
Chr4 exon 78628104 78628330 78628104 ID=MsG0480022923.01.T01:exon:5988;Parent=MsG0480022923.01.T01
Chr4 five_prime_UTR 78628234 78628330 78628234 ID=MsG0480022923.01.T01:five_prime_utr;Parent=MsG0480022923.01.T01
Chr4 CDS 78628104 78628233 78628104 ID=MsG0480022923.01.T01:cds;Parent=MsG0480022923.01.T01
Chr4 CDS 78627876 78628012 78627876 ID=MsG0480022923.01.T01:cds;Parent=MsG0480022923.01.T01
Chr4 CDS 78626613 78626912 78626613 ID=MsG0480022923.01.T01:cds;Parent=MsG0480022923.01.T01
Chr4 three_prime_UTR 78626561 78626612 78626561 ID=MsG0480022923.01.T01:three_prime_utr;Parent=MsG0480022923.01.T01
Chr4 three_prime_UTR 78625919 78626013 78625919 ID=MsG0480022923.01.T01:three_prime_utr;Parent=MsG0480022923.01.T01
Chr4 three_prime_UTR 78625548 78625828 78625548 ID=MsG0480022923.01.T01:three_prime_utr;Parent=MsG0480022923.01.T01
Chr4 three_prime_UTR 78624109 78625409 78624109 ID=MsG0480022923.01.T01:three_prime_utr;Parent=MsG0480022923.01.T01
Chr4 mRNA 78624380 78626053 78624380 ID=MsG0480022923.01.T02;Parent=MsG0480022923.01;Name=MsG0480022923.01.T02;_AED=0.37;_eAED=0.37;_QI=0|0.33|0|1|1|1|4|0|390
Chr4 exon 78625919 78626053 78625919 ID=MsG0480022923.01.T02:exon:5997;Parent=MsG0480022923.01.T02
Chr4 exon 78625697 78625828 78625697 ID=MsG0480022923.01.T02:exon:5996;Parent=MsG0480022923.01.T02
Chr4 exon 78625006 78625409 78625006 ID=MsG0480022923.01.T02:exon:5995;Parent=MsG0480022923.01.T02
Chr4 exon 78624380 78624881 78624380 ID=MsG0480022923.01.T02:exon:5994;Parent=MsG0480022923.01.T02
Chr4 CDS 78625919 78626053 78625919 ID=MsG0480022923.01.T02:cds;Parent=MsG0480022923.01.T02
Chr4 CDS 78625697 78625828 78625697 ID=MsG0480022923.01.T02:cds;Parent=MsG0480022923.01.T02
Chr4 CDS 78625006 78625409 78625006 ID=MsG0480022923.01.T02:cds;Parent=MsG0480022923.01.T02
Chr4 CDS 78624380 78624881 78624380 ID=MsG0480022923.01.T02:cds;Parent=MsG0480022923.01.T02
Gene Sequence

>MsG0480022923.01.T01

ATGGCTCCGAAGCCCAAACCGACGCAGTCGCAGCCTCCCGCTCCACCTCCACTCGAAGATCTATTCACCACTCTCAACAAACACATCCAAGCTTCCGCATTCACCAATGCCGTTAAACTTACAGATCAGATTCTCGCAATTGCACTTGGCGATGAAGACGCACTCCGATGCAAGATCGTTGCGTTGATCAAAGATGATCGATTTGGTGATGCTGTTTCGGCAATTCAATCTTCTCGGTCTCCTCCTGAGGATTTTCATTTTTTTAAGGCATACTGCTTATACAAACAAAACAAGTTGAATGAAGCTTTGGAGTCTTTGGAAAAACATGAGAGAAATGATGAAACTATGCTTTTGGAATCTCAAATTTTGTATCGTCTTGGAAAAATGGATGCTTGCCTGGATATCTACCAGAAGCTTCAAAAGTCCAAGATTGATATGTTGGAAATTAATTCTGTTGCTGCCTTAGTTTGTATGCAGGGATCATCTGAAGTTCAAGGATGGCTGGATTCATTTCGGGTTAAAGCAACAAGCAGCTTTGAATTGGCATACATACTGCTTGTTCTTTAA

>MsG0480022923.01.T02

ATGAATGGCATATACTTATTATTTATATTTTTTCTATCAGAATTGGTCAGAGAGCCTGATGGAAGATACTGGCCTGATGAAGATATAGAAAATGAATTGTCTCCTATTGCTGTACAATTGGGCTATGTCCAACAGCTTCTTGGGCGTAAACAAGATGCTATTGAAGCTTATGTGGACATGATTAAACGAGATATGGCCGATGAATCGTCCATTGCAGTTGCAGTCAATAATCTTGTTTCCCTGAAAGGTCCAAAGATGTTTCTGATAGCTCGAGAACTTGTTTCTGCATTACCAGACATGTTTCCTGATAGTGTTGTACCAGTATTGCTGCAAGCTGCCCTCTTGGTTAGAGAGAACAAAGCTGGAAGGGCTGAGGAAATACTGGCACAGTTTGCTGGAAAGTTGCCTGAGAAGTCCCAGGTTCTTTACTTAGCCAGGGCTCAGGTTGCGGCGGCGGCTGGCCACCCACATATTGCAGCCGATTCTCTAACAAAGATACCAGATATTCAACATATGCCGGCTACTGTTGCCACCCTTGTGTCTTTAAAAGAACGAGCCAACGACGTTGAGGGTGCAGCTGCTGTGCTTGATTCTGCAACCAAGTGGTGGTCTAATGCTATGACTGAGGACAATAAGCTTAATATTATAATGCAAGAGGCTGCCTCATTCAAAGCAGAGCTATATGAAAAGAAACTAAAGACCTTGCCTGGATTGAAGGGAATTGATGTGGACAGCTTGGAGCGTACTTCTGGAGTTAAACAAGTTGAGGGTCCTCTTCATGCAGGTGTTGCCGAAACGAACGACGAAGGAAAGAATAAGACAAAAACTAAGAAAAAGAGAAAGAGAAAGCCAAGGTATCCTAAAGGGTTTAACCCTGCAAACCCAGGTCCTCCACCAGATCCAGAAAGGTGGCTTCCCAAGCGAGAGAGGTCAACTTTTAGGCCCAGAGGAAGAACAACACGAGCTGCTCAAGTTAGAGGCTCACAGGGTGCTGTAGTTAGAGATAAGCAGGAAGCTGGTGCTTCTTCCAGTCATTCCAATCCACCAATCAACTTCCAGTCGCAACTTCCTCCAAAGGTGCTACTCAGAGTGCAGTTTCTGAGCAAACCAAGCCTTCATCCAAGCCTTCATCCAAGTCTAGAAAGAAGTCTAGGAAGTAATAAGGATTTGTGA
Protein sequence

>MsG0480022923.01.T01

MAPKPKPTQSQPPAPPPLEDLFTTLNKHIQASAFTNAVKLTDQILAIALGDEDALRCKIVALIKDDRFGDAVSAIQSSRSPPEDFHFFKAYCLYKQNKLNEALESLEKHERNDETMLLESQILYRLGKMDACLDIYQKLQKSKIDMLEINSVAALVCMQGSSEVQGWLDSFRVKATSSFELAYILLVL*

>MsG0480022923.01.T02

MNGIYLLFIFFLSELVREPDGRYWPDEDIENELSPIAVQLGYVQQLLGRKQDAIEAYVDMIKRDMADESSIAVAVNNLVSLKGPKMFLIARELVSALPDMFPDSVVPVLLQAALLVRENKAGRAEEILAQFAGKLPEKSQVLYLARAQVAAAAGHPHIAADSLTKIPDIQHMPATVATLVSLKERANDVEGAAAVLDSATKWWSNAMTEDNKLNIIMQEAASFKAELYEKKLKTLPGLKGIDVDSLERTSGVKQVEGPLHAGVAETNDEGKNKTKTKKKRKRKPRYPKGFNPANPGPPPDPERWLPKRERSTFRPRGRTTRAAQVRGSQGAVVRDKQEAGASSSHSNPPINFQSQLPPKVLLRVQFLSKPSLHPSLHPSLERSLGSNKDL*