AlfalfaGEDB | MsG0180004185.01

M. sativa cultivar ZhongmuNo.1 / MsG0180004185.01

KEGG: K16732
GO: -
PFAM: PF03999

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004185.01.T01	KAG5004477.1	75.132	378	85	4	1	376	276	646	0	563

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004185.01.T01	Q9FHM4	57.925	347	83	3	1	347	262	545	3.22E-115	354

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004185.01.T01	A0A072VLD1	75.798	376	28	3	1	376	259	571	2.72e-180	528

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047815.01	MsG0180004185.01	0.866807	2.175951e-65	7.430983e-62
MsG0080048258.01	MsG0180004185.01	0.835483	1.634437e-56	2.001429e-53
MsG0080048289.01	MsG0180004185.01	0.806014	1.010399e-49	5.425356e-47
MsG0080048775.01	MsG0180004185.01	0.845129	4.923644e-59	8.123395e-56
MsG0080048781.01	MsG0180004185.01	0.869625	2.691043e-66	1.016663e-62
MsG0080048797.01	MsG0180004185.01	0.820169	7.905454e-53	6.209074e-50
MsG0180001293.01	MsG0180004185.01	0.903893	2.165288e-79	3.267022e-75
MsG0180001719.01	MsG0180004185.01	0.848782	4.920161e-60	9.131105e-57
MsG0180002134.01	MsG0180004185.01	0.816323	5.868514e-52	4.145506e-49
MsG0180002218.01	MsG0180004185.01	0.837270	5.737444e-57	7.413725e-54

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004185.01.T01	MTR_1g076700	75.798	376	28	3	1	376	259	571	0.0	528
MsG0180004185.01.T01	MTR_6g061690	56.649	376	93	5	1	376	261	566	1.07e-128	386
MsG0180004185.01.T01	MTR_7g075220	48.378	339	106	5	1	337	566	837	2.23e-87	285
MsG0180004185.01.T01	MTR_5g093860	41.420	338	130	5	1	333	262	536	1.17e-68	227
MsG0180004185.01.T01	MTR_1g038840	36.152	343	156	3	1	343	231	510	2.37e-64	215
MsG0180004185.01.T01	MTR_1g038840	36.152	343	156	3	1	343	271	550	3.53e-64	216
MsG0180004185.01.T01	MTR_8g041400	35.372	376	167	6	1	371	261	565	1.24e-54	189
MsG0180004185.01.T01	MTR_3g085440	31.378	392	187	10	1	377	266	590	1.69e-47	171
MsG0180004185.01.T01	MTR_3g085440	34.956	226	85	4	1	226	266	429	8.60e-31	122
MsG0180004185.01.T01	MTR_8g023220	30.970	268	138	5	43	310	276	496	7.20e-28	115

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004185.01.T01	AT5G51600	57.925	347	83	3	1	347	262	545	3.28e-116	354
MsG0180004185.01.T01	AT3G60840	47.564	349	112	7	1	346	258	538	6.62e-88	280
MsG0180004185.01.T01	AT5G62250	47.965	344	103	7	1	343	263	531	9.85e-74	239
MsG0180004185.01.T01	AT5G62250	47.965	344	103	7	1	343	263	531	9.85e-74	239
MsG0180004185.01.T01	AT5G55230	42.773	339	153	6	1	333	261	564	1.95e-69	229
MsG0180004185.01.T01	AT5G55230	41.298	339	129	6	1	333	261	535	1.14e-65	219
MsG0180004185.01.T01	AT5G55230	41.298	339	129	6	1	333	261	535	1.14e-65	219
MsG0180004185.01.T01	AT4G26760	39.528	339	135	7	1	333	261	535	4.01e-65	218
MsG0180004185.01.T01	AT1G14690	33.850	387	178	9	1	375	271	591	3.85e-61	207
MsG0180004185.01.T01	AT1G14690	33.850	387	178	9	1	375	271	591	3.85e-61	207

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Find 121 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAAGAAACACCATCATC+CGG	0.474834	1:-74645973	None:intergenic
AAAGAAGAGAACAGAGCTGG+AGG	0.632727	1:+74644409	MsG0180004185.01.T01:CDS
AAAGCCCCAGAGTGTAAAGA+AGG	0.521777	1:+74645801	MsG0180004185.01.T01:CDS
AACAAGAAACACCATCATCC+GGG	0.558335	1:-74645972	None:intergenic
AACATCTGCTGCTCTTCAAT+AGG	0.476308	1:-74644152	None:intergenic
AACTGTTCAAGCACACATGC+AGG	0.479534	1:-74644614	None:intergenic
AAGAAGTATTAATGCCATGC+AGG	0.556928	1:+74644325	MsG0180004185.01.T01:CDS
AAGCCCCAGAGTGTAAAGAA+GGG	0.647810	1:+74645802	MsG0180004185.01.T01:CDS
AAGCGAGCTGAGAAAGCACG+TGG	0.644154	1:+74644893	MsG0180004185.01.T01:CDS
AAGGGACCTAGGATGTCAAC+CGG	0.537940	1:+74645820	MsG0180004185.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATAGTTCCAAAAGCAAGAA+TGG	+	Chr1:74645098-74645117	MsG0180004185.01.T01:intron	30.0%
AGAGATTGAATATTCCTTTG+AGG	+	Chr1:74646996-74647015	MsG0180004185.01.T01:three_prime_UTR	30.0%
AGCATCAATTCCTATGAATA+TGG	+	Chr1:74646876-74646895	MsG0180004185.01.T01:three_prime_UTR	30.0%
ATAGTTCCAAAAGCAAGAAT+GGG	+	Chr1:74645099-74645118	MsG0180004185.01.T01:intron	30.0%
CAAAGGAATATTCAATCTCT+TGG	-	Chr1:74646996-74647015	None:intergenic	30.0%
CAAGTTGCTTGAGTATAATA+TGG	+	Chr1:74644265-74644284	MsG0180004185.01.T01:CDS	30.0%
CAGATAAATAAAATGTTGCC+TGG	-	Chr1:74645639-74645658	None:intergenic	30.0%
CCATCCTATATATAAAAAGG+GGG	-	Chr1:74645253-74645272	None:intergenic	30.0%
GACTTCAAAAACTATAGCAT+GGG	+	Chr1:74645283-74645302	MsG0180004185.01.T01:intron	30.0%
GATACATTATTAGATGAGAG+GGG	-	Chr1:74646676-74646695	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	74644143	74647050	74644143	ID=MsG0180004185.01;Name=MsG0180004185.01
Chr1	mRNA	74644143	74647050	74644143	ID=MsG0180004185.01.T01;Parent=MsG0180004185.01;Name=MsG0180004185.01.T01;_AED=0.47;_eAED=0.65;_QI=0\|0.5\|0.2\|1\|1\|0.8\|5\|474\|386
Chr1	exon	74644143	74644754	74644143	ID=MsG0180004185.01.T01:exon:5624;Parent=MsG0180004185.01.T01
Chr1	exon	74644848	74645012	74644848	ID=MsG0180004185.01.T01:exon:5625;Parent=MsG0180004185.01.T01
Chr1	exon	74645470	74645544	74645470	ID=MsG0180004185.01.T01:exon:5626;Parent=MsG0180004185.01.T01
Chr1	exon	74645730	74646037	74645730	ID=MsG0180004185.01.T01:exon:5627;Parent=MsG0180004185.01.T01
Chr1	exon	74646576	74647050	74646576	ID=MsG0180004185.01.T01:exon:5628;Parent=MsG0180004185.01.T01
Chr1	CDS	74644143	74644754	74644143	ID=MsG0180004185.01.T01:cds;Parent=MsG0180004185.01.T01
Chr1	CDS	74644848	74645012	74644848	ID=MsG0180004185.01.T01:cds;Parent=MsG0180004185.01.T01
Chr1	CDS	74645470	74645544	74645470	ID=MsG0180004185.01.T01:cds;Parent=MsG0180004185.01.T01
Chr1	CDS	74645730	74646037	74645730	ID=MsG0180004185.01.T01:cds;Parent=MsG0180004185.01.T01
Chr1	CDS	74646576	74646576	74646576	ID=MsG0180004185.01.T01:cds;Parent=MsG0180004185.01.T01
Chr1	three_prime_UTR	74646577	74647050	74646577	ID=MsG0180004185.01.T01:three_prime_utr;Parent=MsG0180004185.01.T01

Gene Sequence

>MsG0180004185.01.T01

ATGGATACACCTATTGAAGAGCAGCAGATGTTTCAGAATGTTACTTGTAATATTGCTGCCTCAGAAGATGAAATAACTGAACCAAACACCTTGTCCGAGGACTTCATCAATTGCGTGAGTTACAAGTTGCTTGAGTATAATATGGACTATATTTCTTTATTTTATACGGTTCAGAATTCACGAAGAAGTATTAATGCCATGCAGGTGGAGGTCGAAGTATCTAGGTTAGAAGAGTTAAAATCCAGCAAAATGAAGGAGCTTGTGCTAAAGAAGAGAACAGAGCTGGAGGAAATTTGTCGAAAGACTCATTTAGTTCCAGAAATAGATGGTGCAGTCGAATATGCCGTTGAAGCTATAGAATCTGGTAATATTCTATCTATTTTAATTTGCAAGGTTTTTCTGGACATGATTGCAATTATTTCTTTTGCACACTTCAAACTCTGCCTGTTCCTTGATGCAGGAATTGTGGACCCTGCATGTGTGCTTGAACAGTTTGAGCGCCAGGTTGCCCAAGTTAAAGAGGAAGCTTTGGGTCGAAAAGACATACTTGAAAAGGTTGAGAAATGGTTGGCAGCATGTAATGAAGAGTCTTGGCTTGAGGAATATAACAGGGATGACAATCGATATAATGCTGGAAGAGGTGCTCATCTTACTCTCAAGCGAGCTGAGAAAGCACGTGGGTTGGTTAACAAAATTCCAGGTATTTTTATAGATCTTAATTGTTACGCATATGTTATGGCTCAACGATATGAACACATCAAACTGAATCATGAACCTATTCGTCTACTCTCAATGATTGAAGAGTACAATATATTACGACAAGAGAAAGAGCAGGAACGTCGTAGGCAGCGGGACCTGAAGAAGCTTCAGGGACAAATGATAGCAGAACAGGAGGCACTATATGGATCAAAACCAAGCCCATCAAAGCCCCAGAGTGTAAAGAAGGGACCTAGGATGTCAACCGGAGGGGCAGCTAGTCGAAGAGTCTCCATGGGAGGAGCAATGCTTCAAACTCCAAAACCTGATTCAAAAGCTACTCATTCACGTGCCGTGAGAAAGGTTGACAAAGTGCCTCAGTTTGATCAACTAAGTTACCCGGATGATGGTGTTTCTTGTTTATCATCAGGTAGTTTAACTCTGCTTTTGGTTATACTTTTGTGA

Protein sequence

>MsG0180004185.01.T01

MDTPIEEQQMFQNVTCNIAASEDEITEPNTLSEDFINCVSYKLLEYNMDYISLFYTVQNSRRSINAMQVEVEVSRLEELKSSKMKELVLKKRTELEEICRKTHLVPEIDGAVEYAVEAIESGNILSILICKVFLDMIAIISFAHFKLCLFLDAGIVDPACVLEQFERQVAQVKEEALGRKDILEKVEKWLAACNEESWLEEYNRDDNRYNAGRGAHLTLKRAEKARGLVNKIPGIFIDLNCYAYVMAQRYEHIKLNHEPIRLLSMIEEYNILRQEKEQERRRQRDLKKLQGQMIAEQEALYGSKPSPSKPQSVKKGPRMSTGGAASRRVSMGGAMLQTPKPDSKATHSRAVRKVDKVPQFDQLSYPDDGVSCLSSGSLTLLLVILL*