AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180002218.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002218.01.T01 MTR_1g050472 99.679 312 1 0 1 312 19 330 0.0 640
MsG0180002218.01.T01 MTR_0268s0080 97.115 312 9 0 1 312 19 330 0.0 630
MsG0180002218.01.T01 MTR_7g029110 96.154 312 12 0 1 312 19 330 0.0 604
MsG0180002218.01.T01 MTR_8g469010 92.628 312 18 1 1 312 19 325 0.0 597
MsG0180002218.01.T01 MTR_4g117370 77.564 312 69 1 1 312 31 341 0.0 518
MsG0180002218.01.T01 MTR_6g005270 67.442 43 14 0 247 289 25 67 1.01e-13 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002218.01.T01 AT3G27060 84.076 314 48 2 1 312 19 332 0.0 526
MsG0180002218.01.T01 AT3G23580 77.244 312 70 1 1 312 31 341 0.0 506

Find 47 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCGCGTTGGTTGGGATGAA+TGG 0.235467 1:+35029314 MsG0180002218.01.T01:CDS
CGCTTCCTTGCGCGTTGGTT+GGG 0.274299 1:+35029306 MsG0180002218.01.T01:CDS
GTTTGTTGCTGATAGGTTAT+TGG 0.303774 1:+35029361 MsG0180002218.01.T01:CDS
TGCAATCTGGAAACCATAAA+AGG 0.328563 1:-35028868 None:intergenic
GCGCGTTGGTTGGGATGAAT+GGG 0.334497 1:+35029315 MsG0180002218.01.T01:CDS
AAGCTGATTGGGCGATGAAA+TGG 0.358474 1:+35029006 MsG0180002218.01.T01:CDS
GCGCTTCCTTGCGCGTTGGT+TGG 0.370769 1:+35029305 MsG0180002218.01.T01:CDS
ACCATAAAAGGCACGTGCTT+CGG 0.379129 1:-35028856 None:intergenic
TTTGTTGCTGATAGGTTATT+GGG 0.379609 1:+35029362 MsG0180002218.01.T01:CDS
ATGTTCAGAATCCGTTTGAT+TGG 0.390491 1:+35029411 MsG0180002218.01.T01:CDS
AATCACTTATATACGTTTCA+AGG 0.405711 1:-35028926 None:intergenic
GTTGCATTTGCTTGTGTTGA+AGG 0.436600 1:+35029062 MsG0180002218.01.T01:CDS
AGGCACGTGCTTCGGAGACT+TGG 0.437951 1:-35028848 None:intergenic
GAGCTTATTTCACGCGATGA+AGG 0.439919 1:+35029164 MsG0180002218.01.T01:CDS
TCTGTTATGAATAGCTTGAA+TGG 0.443380 1:+35029503 MsG0180002218.01.T01:CDS
TCAGAATCCGTTTGATTGGA+TGG 0.443597 1:+35029415 MsG0180002218.01.T01:CDS
GCCGTCGGAGGCGGCGAAGA+AGG 0.452615 1:-35028784 MsG0180002217.01.T01:CDS
TGCGGTCCAGAATGAGGCTT+CGG 0.467026 1:-35028679 MsG0180002217.01.T01:CDS
GAAAATCGCACAGAAACTTC+CGG 0.473049 1:-35029096 MsG0180002219.01.T01:CDS
GAGGACGATGCCGTCGGAGG+CGG 0.474359 1:-35028793 None:intergenic
ATGGAATTGATTTCGCTTCA+AGG 0.479094 1:+35029434 MsG0180002218.01.T01:CDS
ATCAATTCCATCCAATCAAA+CGG 0.480364 1:-35029422 None:intergenic
GATAGGTTATTGGGTGAGCT+TGG 0.486660 1:+35029371 MsG0180002218.01.T01:CDS
TGAAATAAGCTCGTTTGAGA+AGG 0.487505 1:-35029153 MsG0180002219.01.T01:CDS
ATATTGAGTTTGTTGCTGAT+AGG 0.493329 1:+35029354 MsG0180002218.01.T01:CDS
CGCGTTGGTTGGGATGAATG+GGG 0.498034 1:+35029316 MsG0180002218.01.T01:CDS
GTGATGCTGTTGAAATTGAA+AGG 0.499878 1:+35029267 MsG0180002218.01.T01:CDS
GGGTGAGCTTGGTTGTGAAA+AGG 0.505349 1:+35029382 MsG0180002218.01.T01:CDS
GCCTTCTTCGCCGCCTCCGA+CGG 0.505987 1:+35028783 MsG0180002218.01.T01:CDS
TTCTTCTGCGGTCCAGAATG+AGG 0.510696 1:-35028685 MsG0180002217.01.T01:CDS
TGGAATTGATTTCGCTTCAA+GGG 0.520571 1:+35029435 MsG0180002218.01.T01:CDS
CGAGGACATGTGAGATGAAG+TGG 0.543318 1:-35028761 MsG0180002217.01.T01:CDS
TGATGCTGTTGAAATTGAAA+GGG 0.545948 1:+35029268 MsG0180002218.01.T01:CDS
ATGAATAGCTTGAATGGTAA+TGG 0.546534 1:+35029509 MsG0180002218.01.T01:CDS
TTTCCTCCGATCTCAAGCAC+TGG 0.566756 1:+35028715 MsG0180002218.01.T01:CDS
TTCGAGGACGATGCCGTCGG+AGG 0.583990 1:-35028796 None:intergenic
TTCATCCCAACCAACGCGCA+AGG 0.586445 1:-35029311 None:intergenic
TCGTTTGAGAAGGTTAATCC+CGG 0.587906 1:-35029143 MsG0180002219.01.T01:CDS
ATCGTCCTCGAAAATCTCGC+TGG 0.589537 1:+35028807 MsG0180002218.01.T01:CDS
CGGAGGCGGCGAAGAAGGCG+AGG 0.590500 1:-35028779 MsG0180002217.01.T01:CDS
GCGTTGGTTGGGATGAATGG+GGG 0.596821 1:+35029317 MsG0180002218.01.T01:CDS
TTGCCAGTGCTTGAGATCGG+AGG 0.610798 1:-35028718 MsG0180002217.01.T01:CDS
TGACGCGCTTCCTTGCGCGT+TGG 0.621614 1:+35029301 MsG0180002218.01.T01:CDS
CACTGGCAAAACCTCACTGA+CGG 0.643079 1:+35028732 MsG0180002218.01.T01:CDS
AGTGGCGTTCGCCGTCAGTG+AGG 0.654051 1:-35028743 MsG0180002217.01.T01:CDS
GGAAAGATCGACTTCTTCTG+CGG 0.673797 1:-35028697 MsG0180002217.01.T01:CDS
GAAGAGAATACATCTCAGAG+TGG 0.694344 1:-35028902 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATCACTTATATACGTTTCA+AGG - Chr1:35028929-35028948 None:intergenic 25.0%
AATCCAATACCCAAAAATCT+GGG + Chr1:35028641-35028660 MsG0180002218.01.T01:CDS 30.0%
ATCAATTCCATCCAATCAAA+CGG - Chr1:35029425-35029444 None:intergenic 30.0%
ATGAATAGCTTGAATGGTAA+TGG + Chr1:35029509-35029528 MsG0180002218.01.T01:CDS 30.0%
TCTGTTATGAATAGCTTGAA+TGG + Chr1:35029503-35029522 MsG0180002218.01.T01:CDS 30.0%
TGATGCTGTTGAAATTGAAA+GGG + Chr1:35029268-35029287 MsG0180002218.01.T01:CDS 30.0%
TTTGTTGCTGATAGGTTATT+GGG + Chr1:35029362-35029381 MsG0180002218.01.T01:CDS 30.0%
! TGTACATTTCCCAGATTTTT+GGG - Chr1:35028653-35028672 MsG0180002217.01.T01:CDS 30.0%
! TTGTACATTTCCCAGATTTT+TGG - Chr1:35028654-35028673 MsG0180002217.01.T01:CDS 30.0%
!! ATATTGAGTTTGTTGCTGAT+AGG + Chr1:35029354-35029373 MsG0180002218.01.T01:CDS 30.0%
!!! AGATTTTTGGGTATTGGATT+GGG - Chr1:35028641-35028660 MsG0180002217.01.T01:CDS 30.0%
!!! ATTTTCTGGTTGAAAAAACG+TGG + Chr1:35029113-35029132 MsG0180002218.01.T01:CDS 30.0%
AAAAAACGTGGTTTAATGCC+GGG + Chr1:35029125-35029144 MsG0180002218.01.T01:CDS 35.0%
ATGTTCAGAATCCGTTTGAT+TGG + Chr1:35029411-35029430 MsG0180002218.01.T01:CDS 35.0%
CAATCCAATACCCAAAAATC+TGG + Chr1:35028640-35028659 MsG0180002218.01.T01:CDS 35.0%
GAAAAAACGTGGTTTAATGC+CGG + Chr1:35029124-35029143 MsG0180002218.01.T01:CDS 35.0%
GTGATGCTGTTGAAATTGAA+AGG + Chr1:35029267-35029286 MsG0180002218.01.T01:CDS 35.0%
TATCGCGAAAAAAGCTGATT+GGG + Chr1:35028995-35029014 MsG0180002218.01.T01:CDS 35.0%
TGAAATAAGCTCGTTTGAGA+AGG - Chr1:35029156-35029175 MsG0180002219.01.T01:CDS 35.0%
TGCAATCTGGAAACCATAAA+AGG - Chr1:35028871-35028890 None:intergenic 35.0%
! ATGGAATTGATTTCGCTTCA+AGG + Chr1:35029434-35029453 MsG0180002218.01.T01:CDS 35.0%
! GGATGTTTTCAATTGCAATC+TGG - Chr1:35028884-35028903 None:intergenic 35.0%
! GTTGAAGGAATCTTTTTCTC+CGG + Chr1:35029077-35029096 MsG0180002218.01.T01:CDS 35.0%
! GTTTGTTGCTGATAGGTTAT+TGG + Chr1:35029361-35029380 MsG0180002218.01.T01:CDS 35.0%
! TGGAATTGATTTCGCTTCAA+GGG + Chr1:35029435-35029454 MsG0180002218.01.T01:CDS 35.0%
!!! CAGATTTTTGGGTATTGGAT+TGG - Chr1:35028642-35028661 MsG0180002217.01.T01:CDS 35.0%
!!! TGCTGATTTTTGCTTCAAGT+TGG + Chr1:35029535-35029554 MsG0180002218.01.T01:CDS 35.0%
!!! TTTCCCAGATTTTTGGGTAT+TGG - Chr1:35028647-35028666 MsG0180002217.01.T01:CDS 35.0%
GAAAATCGCACAGAAACTTC+CGG - Chr1:35029099-35029118 MsG0180002219.01.T01:CDS 40.0%
GAAGAGAATACATCTCAGAG+TGG - Chr1:35028905-35028924 None:intergenic 40.0%
GTATCGCGAAAAAAGCTGAT+TGG + Chr1:35028994-35029013 MsG0180002218.01.T01:CDS 40.0%
GTTGCATTTGCTTGTGTTGA+AGG + Chr1:35029062-35029081 MsG0180002218.01.T01:CDS 40.0%
TCAGAATCCGTTTGATTGGA+TGG + Chr1:35029415-35029434 MsG0180002218.01.T01:CDS 40.0%
TCGTTTGAGAAGGTTAATCC+CGG - Chr1:35029146-35029165 MsG0180002219.01.T01:CDS 40.0%
! GAAGTTTCTGTGCGATTTTC+TGG + Chr1:35029099-35029118 MsG0180002218.01.T01:CDS 40.0%
!!! AACTTTTTTGAGAAGCGCGT+TGG + Chr1:35029464-35029483 MsG0180002218.01.T01:CDS 40.0%
!!! ACTTTTTTGAGAAGCGCGTT+GGG + Chr1:35029465-35029484 MsG0180002218.01.T01:CDS 40.0%
!!! TCAGCTTTTTTCGCGATACA+TGG - Chr1:35028993-35029012 MsG0180002219.01.T01:CDS 40.0%
AAAAAGCCGAAGCCTCATTC+TGG + Chr1:35028673-35028692 MsG0180002218.01.T01:CDS 45.0%
AAGCTGATTGGGCGATGAAA+TGG + Chr1:35029006-35029025 MsG0180002218.01.T01:CDS 45.0%
GAGCTTATTTCACGCGATGA+AGG + Chr1:35029164-35029183 MsG0180002218.01.T01:CDS 45.0%
GATAGGTTATTGGGTGAGCT+TGG + Chr1:35029371-35029390 MsG0180002218.01.T01:CDS 45.0%
! ACCATAAAAGGCACGTGCTT+CGG - Chr1:35028859-35028878 None:intergenic 45.0%
! ATCTACCAGCGAGATTTTCG+AGG - Chr1:35028815-35028834 None:intergenic 45.0%
! GGAAAGATCGACTTCTTCTG+CGG - Chr1:35028700-35028719 MsG0180002217.01.T01:CDS 45.0%
! GTTTTGCCAGTGCTTGAGAT+CGG - Chr1:35028724-35028743 MsG0180002217.01.T01:CDS 45.0%
!!! CTTTTTTGAGAAGCGCGTTG+GGG + Chr1:35029466-35029485 MsG0180002218.01.T01:CDS 45.0%
ATCGTCCTCGAAAATCTCGC+TGG + Chr1:35028807-35028826 MsG0180002218.01.T01:CDS 50.0%
CACTGGCAAAACCTCACTGA+CGG + Chr1:35028732-35028751 MsG0180002218.01.T01:CDS 50.0%
CGAGGACATGTGAGATGAAG+TGG - Chr1:35028764-35028783 MsG0180002217.01.T01:CDS 50.0%
GGGTGAGCTTGGTTGTGAAA+AGG + Chr1:35029382-35029401 MsG0180002218.01.T01:CDS 50.0%
TTCTTCTGCGGTCCAGAATG+AGG - Chr1:35028688-35028707 MsG0180002217.01.T01:CDS 50.0%
TTTCCTCCGATCTCAAGCAC+TGG + Chr1:35028715-35028734 MsG0180002218.01.T01:CDS 50.0%
! TCCGAAGCACGTGCCTTTTA+TGG + Chr1:35028855-35028874 MsG0180002218.01.T01:CDS 50.0%
!! ATTTTCGAGGACGATGCCGT+CGG - Chr1:35028802-35028821 None:intergenic 50.0%
TGCGGTCCAGAATGAGGCTT+CGG - Chr1:35028682-35028701 MsG0180002217.01.T01:CDS 55.0%
TTCATCCCAACCAACGCGCA+AGG - Chr1:35029314-35029333 None:intergenic 55.0%
TTGCCAGTGCTTGAGATCGG+AGG - Chr1:35028721-35028740 MsG0180002217.01.T01:CDS 55.0%
!! CGCGTTGGTTGGGATGAATG+GGG + Chr1:35029316-35029335 MsG0180002218.01.T01:CDS 55.0%
!! GCGCGTTGGTTGGGATGAAT+GGG + Chr1:35029315-35029334 MsG0180002218.01.T01:CDS 55.0%
!! GCGTTGGTTGGGATGAATGG+GGG + Chr1:35029317-35029336 MsG0180002218.01.T01:CDS 55.0%
!! TGCGCGTTGGTTGGGATGAA+TGG + Chr1:35029314-35029333 MsG0180002218.01.T01:CDS 55.0%
AGGCACGTGCTTCGGAGACT+TGG - Chr1:35028851-35028870 None:intergenic 60.0%
!! CGCTTCCTTGCGCGTTGGTT+GGG + Chr1:35029306-35029325 MsG0180002218.01.T01:CDS 60.0%
AGTGGCGTTCGCCGTCAGTG+AGG - Chr1:35028746-35028765 MsG0180002217.01.T01:CDS 65.0%
TGACGCGCTTCCTTGCGCGT+TGG + Chr1:35029301-35029320 MsG0180002218.01.T01:CDS 65.0%
TTCGAGGACGATGCCGTCGG+AGG - Chr1:35028799-35028818 None:intergenic 65.0%
! GCGCTTCCTTGCGCGTTGGT+TGG + Chr1:35029305-35029324 MsG0180002218.01.T01:CDS 65.0%
GAGGACGATGCCGTCGGAGG+CGG - Chr1:35028796-35028815 None:intergenic 70.0%
GCCTTCTTCGCCGCCTCCGA+CGG + Chr1:35028783-35028802 MsG0180002218.01.T01:CDS 70.0%
CGGAGGCGGCGAAGAAGGCG+AGG - Chr1:35028782-35028801 MsG0180002217.01.T01:CDS 75.0%
GCCGTCGGAGGCGGCGAAGA+AGG - Chr1:35028787-35028806 MsG0180002217.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr1 gene 35028633 35029571 35028633 ID=MsG0180002218.01;Name=MsG0180002218.01
Chr1 mRNA 35028633 35029571 35028633 ID=MsG0180002218.01.T01;Parent=MsG0180002218.01;Name=MsG0180002218.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|312
Chr1 exon 35028633 35029571 35028633 ID=MsG0180002218.01.T01:exon:46034;Parent=MsG0180002218.01.T01
Chr1 CDS 35028633 35029571 35028633 ID=MsG0180002218.01.T01:cds;Parent=MsG0180002218.01.T01
Gene Sequence

>MsG0180002218.01.T01

ATGTTCCCAATCCAATACCCAAAAATCTGGGAAATGTACAAAAAAGCCGAAGCCTCATTCTGGACCGCAGAAGAAGTCGATCTTTCCTCCGATCTCAAGCACTGGCAAAACCTCACTGACGGCGAACGCCACTTCATCTCACATGTCCTCGCCTTCTTCGCCGCCTCCGACGGCATCGTCCTCGAAAATCTCGCTGGTAGATTCATGAAAGAAATCCAAGTCTCCGAAGCACGTGCCTTTTATGGTTTCCAGATTGCAATTGAAAACATCCACTCTGAGATGTATTCTCTTCTCCTTGAAACGTATATAAGTGATTCTGCTGAAAAGAATCGCTTATTTCACGCAATTGAAACAATTCCATGTATCGCGAAAAAAGCTGATTGGGCGATGAAATGGATCGATTCAAGCGATTCTTTTGCAGAAAGAATCGTTGCATTTGCTTGTGTTGAAGGAATCTTTTTCTCCGGAAGTTTCTGTGCGATTTTCTGGTTGAAAAAACGTGGTTTAATGCCGGGATTAACCTTCTCAAACGAGCTTATTTCACGCGATGAAGGATTACACTGTGATTTCGCTTGTTTGTTATACTCACTTCTACGCAAGAAGCTAAGTTCAGAGCGTGTGAAAGAAATTGTTTGTGATGCTGTTGAAATTGAAAGGGAGTTTGTGTGTGACGCGCTTCCTTGCGCGTTGGTTGGGATGAATGGGGGTTTGATGAGTACGTATATTGAGTTTGTTGCTGATAGGTTATTGGGTGAGCTTGGTTGTGAAAAGGTTTACAATGTTCAGAATCCGTTTGATTGGATGGAATTGATTTCGCTTCAAGGGAAAACAAACTTTTTTGAGAAGCGCGTTGGGGAGTATCAGAAAGCTTCTGTTATGAATAGCTTGAATGGTAATGGTGCTGCTGATTTTTGCTTCAAGTTGGATGAAGATTTTTAG

Protein sequence

>MsG0180002218.01.T01

MFPIQYPKIWEMYKKAEASFWTAEEVDLSSDLKHWQNLTDGERHFISHVLAFFAASDGIVLENLAGRFMKEIQVSEARAFYGFQIAIENIHSEMYSLLLETYISDSAEKNRLFHAIETIPCIAKKADWAMKWIDSSDSFAERIVAFACVEGIFFSGSFCAIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSSERVKEIVCDAVEIEREFVCDALPCALVGMNGGLMSTYIEFVADRLLGELGCEKVYNVQNPFDWMELISLQGKTNFFEKRVGEYQKASVMNSLNGNGAADFCFKLDEDF*