AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004927.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004927.01.T01 MTR_1g089630 97.115 312 8 1 1 311 145 456 0.0 635
MsG0180004927.01.T01 MTR_2g020860 71.533 274 77 1 29 302 198 470 4.06e-146 419
MsG0180004927.01.T01 MTR_2g020860 71.533 274 77 1 29 302 216 488 7.16e-146 419
MsG0180004927.01.T01 MTR_1g089630 97.765 179 3 1 1 178 157 335 5.79e-129 373
MsG0180004927.01.T01 MTR_1g089630 98.305 177 2 1 1 176 157 333 1.12e-128 370
MsG0180004927.01.T01 MTR_2g020860 76.871 147 34 0 29 175 198 344 6.11e-80 248
MsG0180004927.01.T01 MTR_2g020860 76.871 147 34 0 29 175 216 362 1.53e-79 247
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004927.01.T01 AT2G48030 68.710 310 85 4 4 306 130 434 8.36e-152 433
MsG0180004927.01.T01 AT3G21530 65.141 284 98 1 24 307 172 454 3.15e-134 389
MsG0180004927.01.T01 AT3G21530 65.141 284 98 1 24 307 172 454 3.15e-134 389
MsG0180004927.01.T01 AT3G21530 65.141 284 98 1 24 307 172 454 3.15e-134 389
MsG0180004927.01.T01 AT2G48030 69.663 178 46 2 4 178 130 302 3.36e-84 256
MsG0180004927.01.T01 AT3G21530 67.974 153 49 0 24 176 172 324 1.76e-73 229

Find 115 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTCTCATCTAAACTTATT+TGG 0.134383 1:+84495280 None:intergenic
GGTTTGTTTCAATATGCTTT+TGG 0.157823 1:+84498547 None:intergenic
CTAATAAGGTAGAGTATAAA+AGG 0.213672 1:+84499115 None:intergenic
CAATATCTGTCCATCTTTCT+TGG 0.249809 1:+84495947 None:intergenic
CTTCTCATGTTCCGAGAAAC+TGG 0.256833 1:+84498421 None:intergenic
GTGAAAGCAGAAGAAGAAAA+TGG 0.284924 1:-84498293 MsG0180004927.01.T01:CDS
CACTCTTCACATGGTGATAA+TGG 0.287423 1:+84498375 None:intergenic
AAAGATTGCTTTGTCTAATA+AGG 0.288868 1:+84499101 None:intergenic
ATTGAATAGTGTGGCTTGTT+AGG 0.302936 1:+84499027 None:intergenic
CAATTTGAAGAATGCTCTAT+TGG 0.307677 1:+84495233 None:intergenic
TGTATTTGTACTTTGGCCTT+TGG 0.308377 1:+84495678 None:intergenic
TGAGTACCCATTTGAGTGTT+TGG 0.333270 1:+84498731 None:intergenic
AAGTTTAGTTCACCTGCTTC+AGG 0.343396 1:+84496099 None:intergenic
TCTTTGATCACACCGATATC+AGG 0.345568 1:-84498142 MsG0180004927.01.T01:intron
TATGAGGCTCGTCGTTTGAT+TGG 0.352336 1:+84496013 None:intergenic
TTGACATTCCAACGTTTGAT+AGG 0.354621 1:+84498170 None:intergenic
GAAGATGAAAATAACGTTGT+TGG 0.358702 1:-84498625 MsG0180004927.01.T01:five_prime_UTR
GAAAGCTGGGCGCCGGAGTA+TGG 0.360414 1:-84498215 MsG0180004927.01.T01:CDS
CCAACGTTTGATAGGCCATT+TGG 0.361400 1:+84498178 None:intergenic
GAGGGTTTCAATCAATTTAC+CGG 0.365130 1:-84498477 MsG0180004927.01.T01:five_prime_UTR
GAAGTTGATGCAGATGTATT+GGG 0.365167 1:-84498326 MsG0180004927.01.T01:CDS
TCACCGTATAAGTTTGTTCC+TGG 0.367943 1:-84495506 MsG0180004927.01.T01:CDS
AAAAGGGGAAGGATCCTTCT+TGG 0.370700 1:+84499132 None:intergenic
AATATCTGTCCATCTTTCTT+GGG 0.382102 1:+84495948 None:intergenic
GTGCAAGGGACATGCAAGTA+TGG 0.389057 1:-84495563 MsG0180004927.01.T01:CDS
TGTTTCATCACTTCAACCTT+TGG 0.393136 1:+84495769 None:intergenic
TCGGATTTAGCGGCGGCGTT+AGG 0.393804 1:-84498257 MsG0180004927.01.T01:CDS
GTTGTTGATTATTGTTACTT+TGG 0.401410 1:+84498801 None:intergenic
ATCACTTCAACCTTTGGTGT+TGG 0.403707 1:+84495775 None:intergenic
TTAGAATAATATAAAAGAAA+AGG 0.407127 1:-84495208 MsG0180004927.01.T01:three_prime_UTR
TCGATCATCTCGATGAGAAT+TGG 0.411482 1:-84496062 MsG0180004927.01.T01:CDS
TTGTGGAGATCATAAGAAAA+TGG 0.431814 1:+84498923 None:intergenic
GAACCAGGAACAAACTTATA+CGG 0.436164 1:+84495503 None:intergenic
AGTTCATCCAAACACTCAAA+TGG 0.439361 1:-84498738 MsG0180004927.01.T01:five_prime_UTR
CGAATCCGTCAAAAGGCATC+TGG 0.440862 1:-84495337 MsG0180004927.01.T01:CDS
AGAAGTTGATGCAGATGTAT+TGG 0.442939 1:-84498327 MsG0180004927.01.T01:CDS
TGATGAAACTAACCTGATAT+CGG 0.445051 1:+84498130 None:intergenic
TTTGGAGGAAAGGACTGAAT+AGG 0.446008 1:+84495481 None:intergenic
GACGAGCCTCATATCTTAGC+TGG 0.446117 1:-84496003 MsG0180004927.01.T01:CDS
GAAGCCATTGTCGGATTTAG+CGG 0.452657 1:-84498267 MsG0180004927.01.T01:CDS
TAATAAGGTAGAGTATAAAA+GGG 0.453286 1:+84499116 None:intergenic
TAGAATAATATAAAAGAAAA+GGG 0.453572 1:-84495207 MsG0180004927.01.T01:three_prime_UTR
ACTTAATTCACTGGATGAAT+CGG 0.460278 1:-84495977 MsG0180004927.01.T01:CDS
TGATGGAGAAATGAGGGTCT+TGG 0.463315 1:+84498899 None:intergenic
AAGCCGACAATCCAGTTTCT+CGG 0.467061 1:-84498432 MsG0180004927.01.T01:CDS
GTGATGATCTGATGTACCTT+TGG 0.472747 1:+84495463 None:intergenic
GTCGTCTCCTCTCCCATCTC+CGG 0.481894 1:-84498849 MsG0180004927.01.T01:five_prime_UTR
GTTTAGTACTATGAAGAGAT+GGG 0.482909 1:-84495802 MsG0180004927.01.T01:intron
TGTTTAGTACTATGAAGAGA+TGG 0.484322 1:-84495803 MsG0180004927.01.T01:intron
AACATCGATATCTCGTTATC+CGG 0.487739 1:+84498458 None:intergenic
GAAATGAGGGTCTTGGTTTG+TGG 0.488502 1:+84498906 None:intergenic
TATTCAGTCCTTTCCTCCAA+AGG 0.490237 1:-84495479 MsG0180004927.01.T01:CDS
CGGATTACTCCCAAGAAAGA+TGG 0.490766 1:-84495957 MsG0180004927.01.T01:CDS
GTTCCGAGAAACTGGATTGT+CGG 0.491088 1:+84498429 None:intergenic
ATTATGTGTTTGCAGAAAGC+TGG 0.501661 1:-84498229 MsG0180004927.01.T01:CDS
TAATATTATGCAGGTGTGCA+AGG 0.502416 1:-84495578 MsG0180004927.01.T01:intron
ACAGATGCTAAGAACTATGC+AGG 0.507169 1:-84495727 MsG0180004927.01.T01:CDS
CCACCAACTCATTTCCAAGA+AGG 0.512692 1:-84499146 MsG0180004927.01.T01:five_prime_UTR
ACACATTCGAATCCGTCAAA+AGG 0.523506 1:-84495344 MsG0180004927.01.T01:CDS
CTGATGTACCTTTGGAGGAA+AGG 0.523944 1:+84495471 None:intergenic
AAATGTAAATTGAAATGAGA+TGG 0.525911 1:+84499051 None:intergenic
GTTCATCCAAACACTCAAAT+GGG 0.525963 1:-84498737 MsG0180004927.01.T01:five_prime_UTR
ATGATTTGGACACCGGAGAT+GGG 0.526066 1:+84498837 None:intergenic
GTTGAGGAGTTTGAGCATGA+TGG 0.528774 1:+84498882 None:intergenic
GATGATTTGGACACCGGAGA+TGG 0.528835 1:+84498836 None:intergenic
AAATGGAATGAAGCCATTGT+CGG 0.532401 1:-84498276 MsG0180004927.01.T01:CDS
ACGAGCCTCATATCTTAGCT+GGG 0.538463 1:-84496002 MsG0180004927.01.T01:CDS
GTTTGCAGAAAGCTGGGCGC+CGG 0.542742 1:-84498222 MsG0180004927.01.T01:CDS
TTGGCCTAGGAATGTTCTGA+AGG 0.550621 1:-84496136 MsG0180004927.01.T01:intron
CCTTCTTGGAAATGAGTTGG+TGG 0.552400 1:+84499146 None:intergenic
GATCCTTCTTGGAAATGAGT+TGG 0.553403 1:+84499143 None:intergenic
AGGTAGAGTATAAAAGGGGA+AGG 0.555387 1:+84499121 None:intergenic
CAGTTTCTCGGAACATGAGA+AGG 0.557512 1:-84498420 MsG0180004927.01.T01:CDS
ATCGGCGATGATTTGGACAC+CGG 0.559604 1:+84498830 None:intergenic
AGGAAAGAGCGTCAACGAGT+GGG 0.560733 1:-84498400 MsG0180004927.01.T01:CDS
AAAGAGTTAATTGAATAGTG+TGG 0.561197 1:+84499018 None:intergenic
TTGGACACCGGAGATGGGAG+AGG 0.562500 1:+84498842 None:intergenic
CAGATGCTAAGAACTATGCA+GGG 0.563407 1:-84495726 MsG0180004927.01.T01:CDS
TTGAGCATGATGGAGAAATG+AGG 0.565924 1:+84498892 None:intergenic
ATGTTCTTCCACTCTTCACA+TGG 0.569163 1:+84498366 None:intergenic
AATAAGGTAGAGTATAAAAG+GGG 0.574751 1:+84499117 None:intergenic
ATGATCTGATGTACCTTTGG+AGG 0.576983 1:+84495466 None:intergenic
CATTATCACCATGTGAAGAG+TGG 0.579497 1:-84498374 MsG0180004927.01.T01:CDS
ACTATCGATGTACCTGAAGC+AGG 0.581322 1:-84496111 MsG0180004927.01.T01:CDS
AAGATGGACAGATATTGTAA+AGG 0.584695 1:-84495941 MsG0180004927.01.T01:intron
GAAAGGACTGAATAGGAACC+AGG 0.586709 1:+84495488 None:intergenic
GACATGCAAGTATGGAACTC+GGG 0.589656 1:-84495555 MsG0180004927.01.T01:CDS
GATGGGAAAGCCAACACCAA+AGG 0.591264 1:-84495785 MsG0180004927.01.T01:CDS
GAAGAGTGGAAGAACATTGG+TGG 0.600555 1:-84498360 MsG0180004927.01.T01:CDS
AATATTATGCAGGTGTGCAA+GGG 0.605007 1:-84495577 MsG0180004927.01.T01:intron
GCCATTGTCGGATTTAGCGG+CGG 0.605623 1:-84498264 MsG0180004927.01.T01:CDS
TGAGCATGATGGAGAAATGA+GGG 0.608222 1:+84498893 None:intergenic
TGAGTTAATGTCGAATGTGT+TGG 0.609267 1:+84498597 None:intergenic
AAGGAAAGAGCGTCAACGAG+TGG 0.609973 1:-84498401 MsG0180004927.01.T01:CDS
GAAAGTTGATGTGATGATCA+AGG 0.610675 1:-84495435 MsG0180004927.01.T01:CDS
GGACATGCAAGTATGGAACT+CGG 0.611293 1:-84495556 MsG0180004927.01.T01:CDS
TTATGTGTTTGCAGAAAGCT+GGG 0.614536 1:-84498228 MsG0180004927.01.T01:CDS
GCGGCGTTGAAAGTTGCCAC+CGG 0.617779 1:+84498691 None:intergenic
CCAAATGGCCTATCAAACGT+TGG 0.622562 1:-84498178 MsG0180004927.01.T01:CDS
GTTAACAAAATCGAAAACGA+GGG 0.627972 1:-84498495 MsG0180004927.01.T01:five_prime_UTR
CGAGCCTCATATCTTAGCTG+GGG 0.630468 1:-84496001 MsG0180004927.01.T01:CDS
TCAGTTGTCATGATTGCCAA+AGG 0.632021 1:-84495694 MsG0180004927.01.T01:CDS
GGAAAGAGCGTCAACGAGTG+GGG 0.634833 1:-84498399 MsG0180004927.01.T01:CDS
AAAACAGCATTACCATACTC+CGG 0.640067 1:+84498203 None:intergenic
GGAGATGGGAGAGGAGACGA+CGG 0.658325 1:+84498851 None:intergenic
AGTTAACAAAATCGAAAACG+AGG 0.674649 1:-84498496 MsG0180004927.01.T01:five_prime_UTR
GCCGCCGCTAAATCCGACAA+TGG 0.677798 1:+84498263 None:intergenic
TGTGAAGAGTGGAAGAACAT+TGG 0.688866 1:-84498363 MsG0180004927.01.T01:CDS
AATTCTCATCGAGATGATCG+AGG 0.690165 1:+84496064 None:intergenic
GGCATCTGGAAAACACACAA+CGG 0.692212 1:-84495323 MsG0180004927.01.T01:CDS
GAGCCTCATATCTTAGCTGG+GGG 0.697317 1:-84496000 MsG0180004927.01.T01:CDS
ACACATTCGACATTAACTCA+CGG 0.698946 1:-84498594 MsG0180004927.01.T01:five_prime_UTR
AGATGCTAAGAACTATGCAG+GGG 0.710122 1:-84495725 MsG0180004927.01.T01:CDS
TGGTGCCATTGAGAAAAGTG+CGG 0.723757 1:+84498672 None:intergenic
GATGGGAGAGGAGACGACGG+AGG 0.725633 1:+84498854 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGAATAATATAAAAGAAAA+GGG - Chr1:84499108-84499127 MsG0180004927.01.T01:five_prime_UTR 10.0%
!! TTAGAATAATATAAAAGAAA+AGG - Chr1:84499107-84499126 MsG0180004927.01.T01:five_prime_UTR 10.0%
!! TTATAATAATCAAATTTAGA+AGG - Chr1:84498028-84498047 MsG0180004927.01.T01:intron 10.0%
!! TTCTTAAAGATTAATTATTA+TGG - Chr1:84496557-84496576 MsG0180004927.01.T01:intron 10.0%
!!! TTATAATTTTCAAATTATTC+TGG + Chr1:84497968-84497987 None:intergenic 10.0%
!! AATTATGTGAATTATATACA+TGG + Chr1:84496239-84496258 None:intergenic 15.0%
!! ATTATATACATGGTAAAAAT+TGG + Chr1:84496229-84496248 None:intergenic 15.0%
!! GGTAAAAAGTAAATAAAAAT+AGG - Chr1:84496606-84496625 MsG0180004927.01.T01:intron 15.0%
!! TAAAACAGAATGAAATAAAA+AGG + Chr1:84497103-84497122 None:intergenic 15.0%
!! TAAATAAAAATAGGATAAGT+TGG - Chr1:84496615-84496634 MsG0180004927.01.T01:intron 15.0%
!! TTATATACATGGTAAAAATT+GGG + Chr1:84496228-84496247 None:intergenic 15.0%
!! TTATTCAAGAAAAATATGAA+TGG - Chr1:84497744-84497763 MsG0180004927.01.T01:intron 15.0%
!! TTATTCTAAACAAATGATTA+AGG - Chr1:84497464-84497483 MsG0180004927.01.T01:intron 15.0%
!!! AAAAAATAGTTGATTTGAAA+AGG - Chr1:84497933-84497952 MsG0180004927.01.T01:intron 15.0%
!!! ATGAAAAATTTGTTATTTCT+TGG - Chr1:84498160-84498179 MsG0180004927.01.T01:CDS 15.0%
!!! TTTAAGCTTTTGAAATTAAT+TGG - Chr1:84496704-84496723 MsG0180004927.01.T01:intron 15.0%
!! AAATGTAAATTGAAATGAGA+TGG + Chr1:84495267-84495286 None:intergenic 20.0%
!! AATAAACAAAACAATGAAGA+TGG + Chr1:84497063-84497082 None:intergenic 20.0%
!! AATGTTAGAAACTAATATGA+AGG - Chr1:84496476-84496495 MsG0180004927.01.T01:intron 20.0%
!! ATAACATTTGTTTGTATGAA+AGG - Chr1:84496585-84496604 MsG0180004927.01.T01:intron 20.0%
!! CTAAAATCTTAATATTATGC+AGG - Chr1:84498728-84498747 MsG0180004927.01.T01:five_prime_UTR 20.0%
!! GTGAGATATCTTAATTATTA+TGG - Chr1:84496532-84496551 MsG0180004927.01.T01:intron 20.0%
!! TAATAAGGTAGAGTATAAAA+GGG + Chr1:84495202-84495221 None:intergenic 20.0%
!! TTGTATTTGTATTTGTACTT+TGG + Chr1:84498647-84498666 None:intergenic 20.0%
!!! AAAAGTTTTGCAATAATTTG+TGG + Chr1:84497402-84497421 None:intergenic 20.0%
!!! ATACTTTGTTAGTTTTCTAA+TGG + Chr1:84498097-84498116 None:intergenic 20.0%
!!! CATTGATAATGATATATACA+CGG - Chr1:84497595-84497614 MsG0180004927.01.T01:intron 20.0%
!!! CTGCATAATATTAAGATTTT+AGG + Chr1:84498730-84498749 None:intergenic 20.0%
!!! GAAAACTAACAAAGTATTAA+TGG - Chr1:84498100-84498119 MsG0180004927.01.T01:intron 20.0%
!!! GAAATCTATTTTCTTAGTAT+TGG - Chr1:84498481-84498500 MsG0180004927.01.T01:five_prime_UTR 20.0%
!!! GTTTATGTTTTTTTGTGATT+GGG + Chr1:84495792-84495811 None:intergenic 20.0%
!!! TTTCTGAATTTTAATCATTG+TGG - Chr1:84496773-84496792 MsG0180004927.01.T01:intron 20.0%
! AAAAAAGTAGACAGAAACAA+TGG + Chr1:84497249-84497268 None:intergenic 25.0%
! AAAGAGTTAATTGAATAGTG+TGG + Chr1:84495300-84495319 None:intergenic 25.0%
! AAAGATTGCTTTGTCTAATA+AGG + Chr1:84495217-84495236 None:intergenic 25.0%
! AAGAAGAGTAGGTAAATTAT+TGG + Chr1:84496988-84497007 None:intergenic 25.0%
! AAGTTATGCATAAAACAACA+TGG + Chr1:84497503-84497522 None:intergenic 25.0%
! AATAAGGTAGAGTATAAAAG+GGG + Chr1:84495201-84495220 None:intergenic 25.0%
! AATATATACGTAGTAGTTTC+TGG + Chr1:84497545-84497564 None:intergenic 25.0%
! AATTGGAATCTAGAAAAAAC+AGG - Chr1:84496721-84496740 MsG0180004927.01.T01:intron 25.0%
! ACCAATCACAATTAAAATCT+TGG + Chr1:84496506-84496525 None:intergenic 25.0%
! ATGTTCATCATTGGAATATT+TGG + Chr1:84497793-84497812 None:intergenic 25.0%
! CAAAAACCTGAAAAAAAATC+CGG - Chr1:84495605-84495624 MsG0180004927.01.T01:intron 25.0%
! CCAATCACAATTAAAATCTT+GGG + Chr1:84496505-84496524 None:intergenic 25.0%
! CGTGTATATATCATTATCAA+TGG + Chr1:84497597-84497616 None:intergenic 25.0%
! CTAATAAGGTAGAGTATAAA+AGG + Chr1:84495203-84495222 None:intergenic 25.0%
! TATACAGCTATTAGTATAGT+TGG + Chr1:84497625-84497644 None:intergenic 25.0%
!! AATTTTGAACTTCAACTCTT+CGG - Chr1:84498689-84498708 MsG0180004927.01.T01:five_prime_UTR 25.0%
!! ATAAGAGTATAATGCAATTC+AGG - Chr1:84497984-84498003 MsG0180004927.01.T01:intron 25.0%
!! GTTGTTGATTATTGTTACTT+TGG + Chr1:84495517-84495536 None:intergenic 25.0%
!! GTTTGTTTCAATATGCTTTT+GGG + Chr1:84495770-84495789 None:intergenic 25.0%
!! TTTGTTTCAATATGCTTTTG+GGG + Chr1:84495769-84495788 None:intergenic 25.0%
!!! AAGATTTCCAACTTTTTTGT+TGG - Chr1:84497837-84497856 MsG0180004927.01.T01:intron 25.0%
!!! GGTTTATGTTTTTTTGTGAT+TGG + Chr1:84495793-84495812 None:intergenic 25.0%
!!! TCTTCAAATGTTGTTGTTTT+TGG + Chr1:84495675-84495694 None:intergenic 25.0%
!!! TTGTTACTTTGGATTTTGAT+CGG + Chr1:84495506-84495525 None:intergenic 25.0%
!!! TTGTTTCGTTTTGAGATTTT+GGG + Chr1:84495539-84495558 None:intergenic 25.0%
AAGAGAAAACTAAAAGGAGA+CGG + Chr1:84498438-84498457 None:intergenic 30.0%
AAGAGATATAGAGTGAATGA+AGG + Chr1:84497869-84497888 None:intergenic 30.0%
AAGATGGACAGATATTGTAA+AGG - Chr1:84498374-84498393 MsG0180004927.01.T01:CDS 30.0%
AATAGTATCATCTCAACACA+AGG + Chr1:84497696-84497715 None:intergenic 30.0%
AATATCTGTCCATCTTTCTT+GGG + Chr1:84498370-84498389 None:intergenic 30.0%
ACAGAAACAATGGATGAATA+TGG + Chr1:84497239-84497258 None:intergenic 30.0%
ACTTAATTCACTGGATGAAT+CGG - Chr1:84498338-84498357 MsG0180004927.01.T01:CDS 30.0%
AGACCAAAGAGAAAACTAAA+AGG + Chr1:84498444-84498463 None:intergenic 30.0%
AGTTAACAAAATCGAAAACG+AGG - Chr1:84495819-84495838 MsG0180004927.01.T01:intron 30.0%
AGTTTCTTACTTTACTCCAA+AGG - Chr1:84498051-84498070 MsG0180004927.01.T01:intron 30.0%
ATAGTATCATCTCAACACAA+GGG + Chr1:84497695-84497714 None:intergenic 30.0%
CAATTTGAAGAATGCTCTAT+TGG + Chr1:84499085-84499104 None:intergenic 30.0%
CACTCTCATCTAAACTTATT+TGG + Chr1:84499038-84499057 None:intergenic 30.0%
GAAGATGAAAATAACGTTGT+TGG - Chr1:84495690-84495709 MsG0180004927.01.T01:CDS 30.0%
GAAGTTGTTATGTTCATCAT+TGG + Chr1:84497802-84497821 None:intergenic 30.0%
GAATGAACCAACAAAAAAGT+TGG + Chr1:84497847-84497866 None:intergenic 30.0%
GTGACAGTTCAAAAGATATA+TGG + Chr1:84497426-84497445 None:intergenic 30.0%
GTTAACAAAATCGAAAACGA+GGG - Chr1:84495820-84495839 MsG0180004927.01.T01:intron 30.0%
GTTTAGTACTATGAAGAGAT+GGG - Chr1:84498513-84498532 MsG0180004927.01.T01:five_prime_UTR 30.0%
TCTAGTACATAAAGAAGAGT+AGG + Chr1:84496999-84497018 None:intergenic 30.0%
TGATGAAACTAACCTGATAT+CGG + Chr1:84496188-84496207 None:intergenic 30.0%
TGTTATGAAGTGATTGATTG+TGG - Chr1:84496325-84496344 MsG0180004927.01.T01:intron 30.0%
TGTTTAGTACTATGAAGAGA+TGG - Chr1:84498512-84498531 MsG0180004927.01.T01:five_prime_UTR 30.0%
TTGTGGAGATCATAAGAAAA+TGG + Chr1:84495395-84495414 None:intergenic 30.0%
! AAAATAGGATAAGTTGGCAT+TGG - Chr1:84496621-84496640 MsG0180004927.01.T01:intron 30.0%
! AGAAAATCTTTTTGCTCTTG+TGG - Chr1:84497303-84497322 MsG0180004927.01.T01:intron 30.0%
! CAAAATCCTTAAGGATTAGT+AGG + Chr1:84497670-84497689 None:intergenic 30.0%
! TTACGATTTTCTGTCTATGT+TGG + Chr1:84498946-84498965 None:intergenic 30.0%
! TTGTTTCAATATGCTTTTGG+GGG + Chr1:84495768-84495787 None:intergenic 30.0%
!! AATTTGTTATTTCTTGGCCT+AGG - Chr1:84498166-84498185 MsG0180004927.01.T01:CDS 30.0%
!! GGTTTGTTTCAATATGCTTT+TGG + Chr1:84495771-84495790 None:intergenic 30.0%
!! TTTAATTGTGATTGGTTCTG+AGG - Chr1:84496510-84496529 MsG0180004927.01.T01:intron 30.0%
!!! CCCAAGATTTTAATTGTGAT+TGG - Chr1:84496502-84496521 MsG0180004927.01.T01:intron 30.0%
!!! CTTGTTTCGTTTTGAGATTT+TGG + Chr1:84495540-84495559 None:intergenic 30.0%
!!! TCTCCTTTTAGTTTTCTCTT+TGG - Chr1:84498438-84498457 MsG0180004927.01.T01:CDS 30.0%
AAAACAGCATTACCATACTC+CGG + Chr1:84496115-84496134 None:intergenic 35.0%
AAACCTGAAAAAAAATCCGG+TGG - Chr1:84495608-84495627 MsG0180004927.01.T01:intron 35.0%
AAATGGAATGAAGCCATTGT+CGG - Chr1:84496039-84496058 MsG0180004927.01.T01:CDS 35.0%
AACATCGATATCTCGTTATC+CGG + Chr1:84495860-84495879 None:intergenic 35.0%
AATATTATGCAGGTGTGCAA+GGG - Chr1:84498738-84498757 MsG0180004927.01.T01:five_prime_UTR 35.0%
ACACATTCGACATTAACTCA+CGG - Chr1:84495721-84495740 MsG0180004927.01.T01:CDS 35.0%
AGTTCATCCAAACACTCAAA+TGG - Chr1:84495577-84495596 MsG0180004927.01.T01:intron 35.0%
ATTATGTGTTTGCAGAAAGC+TGG - Chr1:84496086-84496105 MsG0180004927.01.T01:CDS 35.0%
ATTGAGAATCTGATGTGTGA+TGG + Chr1:84498409-84498428 None:intergenic 35.0%
CAATATCTGTCCATCTTTCT+TGG + Chr1:84498371-84498390 None:intergenic 35.0%
GAACCAGGAACAAACTTATA+CGG + Chr1:84498815-84498834 None:intergenic 35.0%
GAGGGTTTCAATCAATTTAC+CGG - Chr1:84495838-84495857 MsG0180004927.01.T01:intron 35.0%
GATGAACATAACAACTTCAG+TGG - Chr1:84497804-84497823 MsG0180004927.01.T01:intron 35.0%
GTGAAAGCAGAAGAAGAAAA+TGG - Chr1:84496022-84496041 MsG0180004927.01.T01:CDS 35.0%
GTTCATCCAAACACTCAAAT+GGG - Chr1:84495578-84495597 MsG0180004927.01.T01:intron 35.0%
TAATATTATGCAGGTGTGCA+AGG - Chr1:84498737-84498756 MsG0180004927.01.T01:five_prime_UTR 35.0%
TAGTATCATCTCAACACAAG+GGG + Chr1:84497694-84497713 None:intergenic 35.0%
TCACGACTAAATGGTATCTA+AGG + Chr1:84497282-84497301 None:intergenic 35.0%
TCATTAGCAAGAGAAACCTT+TGG + Chr1:84498070-84498089 None:intergenic 35.0%
TGAGTTAATGTCGAATGTGT+TGG + Chr1:84495721-84495740 None:intergenic 35.0%
TGTTTCATCACTTCAACCTT+TGG + Chr1:84498549-84498568 None:intergenic 35.0%
TTATGTGTTTGCAGAAAGCT+GGG - Chr1:84496087-84496106 MsG0180004927.01.T01:CDS 35.0%
TTGACATTCCAACGTTTGAT+AGG + Chr1:84496148-84496167 None:intergenic 35.0%
! AATCCTTAAGGATTAGTAGG+TGG + Chr1:84497667-84497686 None:intergenic 35.0%
! ACTAAATGGTATCTAAGGTG+TGG + Chr1:84497277-84497296 None:intergenic 35.0%
! ATCCTTAAGGATTAGTAGGT+GGG + Chr1:84497666-84497685 None:intergenic 35.0%
! ATTTTCTGTCTATGTTGGTG+CGG + Chr1:84498941-84498960 None:intergenic 35.0%
! CAACTGTTTTGTGTTTGTGT+TGG + Chr1:84495346-84495365 None:intergenic 35.0%
! CTAAATGGTATCTAAGGTGT+GGG + Chr1:84497276-84497295 None:intergenic 35.0%
! GATTTTCTCTCACGACTAAA+TGG + Chr1:84497291-84497310 None:intergenic 35.0%
! TATCTTTTGAACTGTCACAC+TGG - Chr1:84497428-84497447 MsG0180004927.01.T01:intron 35.0%
! TCGATTTTGTTAACTGTACG+AGG + Chr1:84495814-84495833 None:intergenic 35.0%
! TGTATTTGTACTTTGGCCTT+TGG + Chr1:84498640-84498659 None:intergenic 35.0%
!! AGAAGTTGATGCAGATGTAT+TGG - Chr1:84495988-84496007 MsG0180004927.01.T01:CDS 35.0%
!! ATTGAATAGTGTGGCTTGTT+AGG + Chr1:84495291-84495310 None:intergenic 35.0%
!! GAAAGTTGATGTGATGATCA+AGG - Chr1:84498880-84498899 MsG0180004927.01.T01:five_prime_UTR 35.0%
!! GAAGTTGATGCAGATGTATT+GGG - Chr1:84495989-84496008 MsG0180004927.01.T01:CDS 35.0%
!!! GTAATGCTGTTTTCTCCAAA+TGG - Chr1:84496122-84496141 MsG0180004927.01.T01:CDS 35.0%
!!! GTTGGTATTGATTTTGTGTG+TGG + Chr1:84495328-84495347 None:intergenic 35.0%
AATTCTCATCGAGATGATCG+AGG + Chr1:84498254-84498273 None:intergenic 40.0%
ACAAGGGGACAAAATCCTTA+AGG + Chr1:84497679-84497698 None:intergenic 40.0%
ACACATTCGAATCCGTCAAA+AGG - Chr1:84498971-84498990 MsG0180004927.01.T01:five_prime_UTR 40.0%
ACAGATGCTAAGAACTATGC+AGG - Chr1:84498588-84498607 MsG0180004927.01.T01:five_prime_UTR 40.0%
AGATGCTAAGAACTATGCAG+GGG - Chr1:84498590-84498609 MsG0180004927.01.T01:five_prime_UTR 40.0%
AGGTAGAGTATAAAAGGGGA+AGG + Chr1:84495197-84495216 None:intergenic 40.0%
ATCCATTGCATGAGAAAGAG+AGG - Chr1:84497349-84497368 MsG0180004927.01.T01:intron 40.0%
ATCCCACCTACTAATCCTTA+AGG - Chr1:84497661-84497680 MsG0180004927.01.T01:intron 40.0%
ATGATCTGATGTACCTTTGG+AGG + Chr1:84498852-84498871 None:intergenic 40.0%
ATGTTCTTCCACTCTTCACA+TGG + Chr1:84495952-84495971 None:intergenic 40.0%
CACTCTTCACATGGTGATAA+TGG + Chr1:84495943-84495962 None:intergenic 40.0%
CAGATGCTAAGAACTATGCA+GGG - Chr1:84498589-84498608 MsG0180004927.01.T01:five_prime_UTR 40.0%
CATTATCACCATGTGAAGAG+TGG - Chr1:84495941-84495960 MsG0180004927.01.T01:intron 40.0%
GATCCTTCTTGGAAATGAGT+TGG + Chr1:84495175-84495194 None:intergenic 40.0%
GTGATGATCTGATGTACCTT+TGG + Chr1:84498855-84498874 None:intergenic 40.0%
TATTCAGTCCTTTCCTCCAA+AGG - Chr1:84498836-84498855 MsG0180004927.01.T01:five_prime_UTR 40.0%
TCAGTTGTCATGATTGCCAA+AGG - Chr1:84498621-84498640 MsG0180004927.01.T01:five_prime_UTR 40.0%
TCGATCATCTCGATGAGAAT+TGG - Chr1:84498253-84498272 MsG0180004927.01.T01:CDS 40.0%
TCTTTGATCACACCGATATC+AGG - Chr1:84496173-84496192 MsG0180004927.01.T01:intron 40.0%
TGAGTACCCATTTGAGTGTT+TGG + Chr1:84495587-84495606 None:intergenic 40.0%
TGTGAAGAGTGGAAGAACAT+TGG - Chr1:84495952-84495971 MsG0180004927.01.T01:CDS 40.0%
TTCCTCTCTTTCTCATGCAA+TGG + Chr1:84497354-84497373 None:intergenic 40.0%
TTTGGAGGAAAGGACTGAAT+AGG + Chr1:84498837-84498856 None:intergenic 40.0%
! AAGTTTAGTTCACCTGCTTC+AGG + Chr1:84498219-84498238 None:intergenic 40.0%
! ATCACTTCAACCTTTGGTGT+TGG + Chr1:84498543-84498562 None:intergenic 40.0%
! GTTTTCCAGATGCCTTTTGA+CGG + Chr1:84498986-84499005 None:intergenic 40.0%
! TCACCGTATAAGTTTGTTCC+TGG - Chr1:84498809-84498828 MsG0180004927.01.T01:five_prime_UTR 40.0%
! TGAGCATGATGGAGAAATGA+GGG + Chr1:84495425-84495444 None:intergenic 40.0%
! TTGAGCATGATGGAGAAATG+AGG + Chr1:84495426-84495445 None:intergenic 40.0%
!! GATTTTGATCGGCGATGATT+TGG + Chr1:84495495-84495514 None:intergenic 40.0%
!!! GTTGTTGTTTTTGGAAGTGC+TGG + Chr1:84495666-84495685 None:intergenic 40.0%
!!! TTGCCACCGGATTTTTTTTC+AGG + Chr1:84495614-84495633 None:intergenic 40.0%
AAAAGGGGAAGGATCCTTCT+TGG + Chr1:84495186-84495205 None:intergenic 45.0%
AAGCCGACAATCCAGTTTCT+CGG - Chr1:84495883-84495902 MsG0180004927.01.T01:intron 45.0%
ACGAGCCTCATATCTTAGCT+GGG - Chr1:84498313-84498332 MsG0180004927.01.T01:CDS 45.0%
ACTATCGATGTACCTGAAGC+AGG - Chr1:84498204-84498223 MsG0180004927.01.T01:CDS 45.0%
ATAGCTGTATACCACGAGAC+AGG - Chr1:84497634-84497653 MsG0180004927.01.T01:intron 45.0%
CAGTTTCTCGGAACATGAGA+AGG - Chr1:84495895-84495914 MsG0180004927.01.T01:intron 45.0%
CCAAATGGCCTATCAAACGT+TGG - Chr1:84496137-84496156 MsG0180004927.01.T01:intron 45.0%
CCAACGTTTGATAGGCCATT+TGG + Chr1:84496140-84496159 None:intergenic 45.0%
CCACCAACTCATTTCCAAGA+AGG - Chr1:84495169-84495188 MsG0180004927.01.T01:three_prime_UTR 45.0%
CGGATTACTCCCAAGAAAGA+TGG - Chr1:84498358-84498377 MsG0180004927.01.T01:CDS 45.0%
CTTCTCATGTTCCGAGAAAC+TGG + Chr1:84495897-84495916 None:intergenic 45.0%
GAAAGGACTGAATAGGAACC+AGG + Chr1:84498830-84498849 None:intergenic 45.0%
GAAGAGTGGAAGAACATTGG+TGG - Chr1:84495955-84495974 MsG0180004927.01.T01:CDS 45.0%
GAAGCCATTGTCGGATTTAG+CGG - Chr1:84496048-84496067 MsG0180004927.01.T01:CDS 45.0%
GACATGCAAGTATGGAACTC+GGG - Chr1:84498760-84498779 MsG0180004927.01.T01:five_prime_UTR 45.0%
GGACATGCAAGTATGGAACT+CGG - Chr1:84498759-84498778 MsG0180004927.01.T01:five_prime_UTR 45.0%
GGCATCTGGAAAACACACAA+CGG - Chr1:84498992-84499011 MsG0180004927.01.T01:five_prime_UTR 45.0%
GTTCCGAGAAACTGGATTGT+CGG + Chr1:84495889-84495908 None:intergenic 45.0%
GTTGAGGAGTTTGAGCATGA+TGG + Chr1:84495436-84495455 None:intergenic 45.0%
GTTGCCTTCAGAACATTCCT+AGG + Chr1:84498186-84498205 None:intergenic 45.0%
TATGAGGCTCGTCGTTTGAT+TGG + Chr1:84498305-84498324 None:intergenic 45.0%
TGATGGAGAAATGAGGGTCT+TGG + Chr1:84495419-84495438 None:intergenic 45.0%
TTGGCCTAGGAATGTTCTGA+AGG - Chr1:84498179-84498198 MsG0180004927.01.T01:CDS 45.0%
! ATGATTTGGACACCGGAGAT+GGG + Chr1:84495481-84495500 None:intergenic 45.0%
! CCTTCTTGGAAATGAGTTGG+TGG + Chr1:84495172-84495191 None:intergenic 45.0%
! CTGATGTACCTTTGGAGGAA+AGG + Chr1:84498847-84498866 None:intergenic 45.0%
! GAAATGAGGGTCTTGGTTTG+TGG + Chr1:84495412-84495431 None:intergenic 45.0%
!! TGGTGCCATTGAGAAAAGTG+CGG + Chr1:84495646-84495665 None:intergenic 45.0%
AGCTGGGGGACTTAATTCAC+TGG - Chr1:84498329-84498348 MsG0180004927.01.T01:CDS 50.0%
AGTCCCCCAGCTAAGATATG+AGG + Chr1:84498321-84498340 None:intergenic 50.0%
CGAGCCTCATATCTTAGCTG+GGG - Chr1:84498314-84498333 MsG0180004927.01.T01:CDS 50.0%
GACGAGCCTCATATCTTAGC+TGG - Chr1:84498312-84498331 MsG0180004927.01.T01:CDS 50.0%
GAGCCTCATATCTTAGCTGG+GGG - Chr1:84498315-84498334 MsG0180004927.01.T01:CDS 50.0%
GATGGGAAAGCCAACACCAA+AGG - Chr1:84498530-84498549 MsG0180004927.01.T01:five_prime_UTR 50.0%
GTGCAAGGGACATGCAAGTA+TGG - Chr1:84498752-84498771 MsG0180004927.01.T01:five_prime_UTR 50.0%
! ATCGGCGATGATTTGGACAC+CGG + Chr1:84495488-84495507 None:intergenic 50.0%
! CAACGCCGCACTTTTCTCAA+TGG - Chr1:84495638-84495657 MsG0180004927.01.T01:intron 50.0%
! GACGACGGAGGTTTTTGTTG+AGG + Chr1:84495452-84495471 None:intergenic 50.0%
! GATGATTTGGACACCGGAGA+TGG + Chr1:84495482-84495501 None:intergenic 50.0%
!! AAGGAAAGAGCGTCAACGAG+TGG - Chr1:84495914-84495933 MsG0180004927.01.T01:intron 50.0%
!! AGGAAAGAGCGTCAACGAGT+GGG - Chr1:84495915-84495934 MsG0180004927.01.T01:intron 50.0%
!! CGAATCCGTCAAAAGGCATC+TGG - Chr1:84498978-84498997 MsG0180004927.01.T01:five_prime_UTR 50.0%
GCCATTGTCGGATTTAGCGG+CGG - Chr1:84496051-84496070 MsG0180004927.01.T01:CDS 55.0%
GTGGGATCAATCCTGTCTCG+TGG + Chr1:84497648-84497667 None:intergenic 55.0%
!! GGAAAGAGCGTCAACGAGTG+GGG - Chr1:84495916-84495935 MsG0180004927.01.T01:intron 55.0%
GCCGCCGCTAAATCCGACAA+TGG + Chr1:84496055-84496074 None:intergenic 60.0%
GGAGATGGGAGAGGAGACGA+CGG + Chr1:84495467-84495486 None:intergenic 60.0%
GTCGTCTCCTCTCCCATCTC+CGG - Chr1:84495466-84495485 MsG0180004927.01.T01:CDS 60.0%
GTTTGCAGAAAGCTGGGCGC+CGG - Chr1:84496093-84496112 MsG0180004927.01.T01:CDS 60.0%
TCGGATTTAGCGGCGGCGTT+AGG - Chr1:84496058-84496077 MsG0180004927.01.T01:CDS 60.0%
TTGGACACCGGAGATGGGAG+AGG + Chr1:84495476-84495495 None:intergenic 60.0%
! GCGGCGTTGAAAGTTGCCAC+CGG + Chr1:84495627-84495646 None:intergenic 60.0%
GATGGGAGAGGAGACGACGG+AGG + Chr1:84495464-84495483 None:intergenic 65.0%
!! GAAAGCTGGGCGCCGGAGTA+TGG - Chr1:84496100-84496119 MsG0180004927.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 84495149 84499188 84495149 ID=MsG0180004927.01;Name=MsG0180004927.01
Chr1 mRNA 84495149 84499188 84495149 ID=MsG0180004927.01.T01;Parent=MsG0180004927.01;Name=MsG0180004927.01.T01;_AED=0.27;_eAED=0.27;_QI=726|1|1|1|1|1|4|161|311
Chr1 exon 84495149 84495587 84495149 ID=MsG0180004927.01.T01:exon:9298;Parent=MsG0180004927.01.T01
Chr1 exon 84495689 84495818 84495689 ID=MsG0180004927.01.T01:exon:9297;Parent=MsG0180004927.01.T01
Chr1 exon 84495942 84496149 84495942 ID=MsG0180004927.01.T01:exon:9296;Parent=MsG0180004927.01.T01
Chr1 exon 84498143 84499188 84498143 ID=MsG0180004927.01.T01:exon:9295;Parent=MsG0180004927.01.T01
Chr1 five_prime_UTR 84498463 84499188 84498463 ID=MsG0180004927.01.T01:five_prime_utr;Parent=MsG0180004927.01.T01
Chr1 CDS 84498143 84498462 84498143 ID=MsG0180004927.01.T01:cds;Parent=MsG0180004927.01.T01
Chr1 CDS 84495942 84496149 84495942 ID=MsG0180004927.01.T01:cds;Parent=MsG0180004927.01.T01
Chr1 CDS 84495689 84495818 84495689 ID=MsG0180004927.01.T01:cds;Parent=MsG0180004927.01.T01
Chr1 CDS 84495310 84495587 84495310 ID=MsG0180004927.01.T01:cds;Parent=MsG0180004927.01.T01
Chr1 three_prime_UTR 84495149 84495309 84495149 ID=MsG0180004927.01.T01:three_prime_utr;Parent=MsG0180004927.01.T01
Gene Sequence

>MsG0180004927.01.T01

ATGTTAAGAAGCCGACAATCCAGTTTCTCGGAACATGAGAAGGAAAGAGCGTCAACGAGTGGGGTCCATTATCACCATGTGAAGAGTGGAAGAACATTGGTGGAAGTGTTGAGAGAAGTTGATGCAGATGTATTGGGATTGCAAGATGTGAAAGCAGAAGAAGAAAATGGAATGAAGCCATTGTCGGATTTAGCGGCGGCGTTAGGAATGAATTATGTGTTTGCAGAAAGCTGGGCGCCGGAGTATGGTAATGCTGTTTTCTCCAAATGGCCTATCAAACGTTGGAATGTCAACAAAATCTTTGATCACACCGATATCAGGAATGTTCTGAAGGCAACTATCGATGTACCTGAAGCAGGTGAACTAAACTTTTACTGCACTCACCTCGATCATCTCGATGAGAATTGGCGAATGAAGCAGATAAATGCAATAATCCAATCAAACGACGAGCCTCATATCTTAGCTGGGGGACTTAATTCACTGGATGAATCGGATTACTCCCAAGAAAGATGGACAGATATTGTAAAGTACTATGAAGAGATGGGAAAGCCAACACCAAAGGTTGAAGTGATGAAACATCTAAAGAGTAAAGACTATACAGATGCTAAGAACTATGCAGGGGAATATGAATCAGTTGTCATGATTGCCAAAGGCCAAAGTGTGCAAGGGACATGCAAGTATGGAACTCGGGTAGATTACATATTATCATCATCGAATTCACCGTATAAGTTTGTTCCTGGTTCCTATTCAGTCCTTTCCTCCAAAGGTACATCAGATCATCACATTGTGAAAGTTGATGTGATGATCAAGGTAAATAACAATTCTGAAGAAAATGCGACGAAGAAACCGCACCAACATAGACAGAAAATCGTAAGAGTAACACATTCGAATCCGTCAAAAGGCATCTGGAAAACACACAACGGAGAAGTATATTGA
Protein sequence

>MsG0180004927.01.T01

MLRSRQSSFSEHEKERASTSGVHYHHVKSGRTLVEVLREVDADVLGLQDVKAEEENGMKPLSDLAAALGMNYVFAESWAPEYGNAVFSKWPIKRWNVNKIFDHTDIRNVLKATIDVPEAGELNFYCTHLDHLDENWRMKQINAIIQSNDEPHILAGGLNSLDESDYSQERWTDIVKYYEEMGKPTPKVEVMKHLKSKDYTDAKNYAGEYESVVMIAKGQSVQGTCKYGTRVDYILSSSNSPYKFVPGSYSVLSSKGTSDHHIVKVDVMIKVNNNSEENATKKPHQHRQKIVRVTHSNPSKGIWKTHNGEVY*