AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022090.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022090.01.T01 MTR_4g082315 97.633 169 4 0 1 169 1 169 2.63e-123 347
MsG0480022090.01.T01 MTR_7g011490 61.111 162 58 1 13 169 4 165 7.63e-71 214
MsG0480022090.01.T01 MTR_6g012770 64.138 145 51 1 26 169 21 165 8.36e-69 208
MsG0480022090.01.T01 MTR_5g042260 52.023 173 78 4 1 169 1 172 7.25e-61 188
MsG0480022090.01.T01 MTR_5g042320 50.282 177 81 4 1 171 3 178 1.31e-52 167
MsG0480022090.01.T01 MTR_5g042320 50.286 175 80 4 1 169 3 176 1.42e-52 167
MsG0480022090.01.T01 MTR_5g042270 46.104 154 74 3 27 177 1 148 5.14e-44 144
MsG0480022090.01.T01 MTR_3g072600 42.331 163 85 4 12 169 12 170 1.68e-39 134
MsG0480022090.01.T01 MTR_5g058140 42.069 145 78 2 25 169 27 165 2.48e-39 133
MsG0480022090.01.T01 MTR_2g007210 41.139 158 88 3 12 169 7 159 8.97e-39 132
MsG0480022090.01.T01 MTR_8g088940 37.500 160 87 3 15 169 13 164 1.39e-31 113
MsG0480022090.01.T01 MTR_8g088980 43.434 99 55 1 72 169 17 115 2.79e-26 98.6
MsG0480022090.01.T01 MTR_5g042300 39.437 71 43 0 99 169 45 115 3.76e-15 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022090.01.T01 AT1G26690 64.103 156 56 0 14 169 12 167 2.45e-75 225
MsG0480022090.01.T01 AT1G69460 64.103 156 56 0 14 169 12 167 1.18e-72 218
MsG0480022090.01.T01 AT1G14010 60.256 156 62 0 14 169 10 165 3.00e-69 209
MsG0480022090.01.T01 AT2G03040 59.864 147 58 1 24 169 20 166 1.52e-61 188
MsG0480022090.01.T01 AT2G03290 59.864 147 58 1 24 169 20 166 2.17e-61 189
MsG0480022090.01.T01 AT3G29070 53.103 145 68 0 25 169 34 178 7.41e-56 176
MsG0480022090.01.T01 AT1G09580 38.415 164 94 3 8 169 12 170 8.36e-37 127
MsG0480022090.01.T01 AT1G21900 35.000 160 99 3 12 169 13 169 1.53e-31 114
MsG0480022090.01.T01 AT1G57620 35.000 160 95 4 14 169 11 165 8.76e-31 111
MsG0480022090.01.T01 AT3G10780 27.815 151 102 2 23 169 23 170 1.32e-17 77.4

Find 49 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTAACGTTGACTATTGATT+TGG 0.321195 4:+68062106 MsG0480022090.01.T01:CDS
GTTAAGAGAAATCATGGTTA+TGG 0.347540 4:-68061633 None:intergenic
TAGTAGTTGGCATCTTTGTT+TGG 0.398165 4:+68061497 MsG0480022090.01.T01:five_prime_UTR
GGTAAAGGGTAACCCTCGTT+GGG 0.404929 4:-68061812 None:intergenic
GATGTCTTCTGAGATGCATT+TGG 0.409852 4:-68061747 None:intergenic
TGACCTTTCTTAGCAACATT+GGG 0.430307 4:-68062161 None:intergenic
AACGGTGATTCTGTGTGAAT+CGG 0.435968 4:-68061834 None:intergenic
AGCAACATTGGGCCAATCCT+TGG 0.436187 4:-68062150 None:intergenic
GAAAGGTACACATGCACAAT+TGG 0.438394 4:+68064635 MsG0480022090.01.T01:CDS
AGTTGGCATCTTTGTTTGGT+TGG 0.443686 4:+68061501 MsG0480022090.01.T01:five_prime_UTR
TATGGAGATCGTGTTCAATC+TGG 0.449834 4:+68062011 MsG0480022090.01.T01:CDS
GCATTTGTTGCTGCAGAAGC+TGG 0.459925 4:+68062041 MsG0480022090.01.T01:CDS
CATGGAAATAACTATCACTA+TGG 0.460188 4:+68061993 MsG0480022090.01.T01:CDS
GTTGAGTTGATCATAGTAGT+TGG 0.470707 4:+68061484 MsG0480022090.01.T01:five_prime_UTR
GGTACACATGCACAATTGGC+TGG 0.475971 4:+68064639 MsG0480022090.01.T01:CDS
TTCTGTGTGAATCGGGTAAA+GGG 0.479747 4:-68061826 None:intergenic
CTCAACAAGGATGTCAAACT+AGG 0.480765 4:-68061467 None:intergenic
CTGGTGTGGCAGCCAAGGAT+TGG 0.480930 4:+68062138 MsG0480022090.01.T01:CDS
ACGGTGATTCTGTGTGAATC+GGG 0.486370 4:-68061833 None:intergenic
CGTTGACTATTGATTTGGAA+TGG 0.486839 4:+68062111 MsG0480022090.01.T01:CDS
ATTCAGTGGAAGATGAAAAC+AGG 0.491337 4:-68061694 None:intergenic
TTCACACAGAATCACCGTTC+GGG 0.491934 4:+68061838 MsG0480022090.01.T01:CDS
AAATCATGGTTATGGAAAAG+TGG 0.496969 4:-68061625 None:intergenic
ATTCTGTGTGAATCGGGTAA+AGG 0.500485 4:-68061827 None:intergenic
AAAAGCAATTCCATGACTGT+TGG 0.505073 4:+68061770 MsG0480022090.01.T01:CDS
GTTTAGGTTACTTCGTCTCA+TGG 0.505101 4:+68061975 MsG0480022090.01.T01:intron
TCAATAGTCAACGTTACCTC+AGG 0.509448 4:-68062101 None:intergenic
TTGACCTTTCTTAGCAACAT+TGG 0.528677 4:-68062162 None:intergenic
GAAGACTGGTGTGGCAGCCA+AGG 0.530610 4:+68062133 MsG0480022090.01.T01:CDS
TGGCCCAATGTTGCTAAGAA+AGG 0.532329 4:+68062158 MsG0480022090.01.T01:CDS
GGGTAAAGGGTAACCCTCGT+TGG 0.534307 4:-68061813 None:intergenic
AAGATTTGAGTTCAAATCTG+AGG 0.534501 4:-68061718 None:intergenic
AGATTTGAGTTCAAATCTGA+GGG 0.538909 4:-68061717 None:intergenic
ATTCACACAGAATCACCGTT+CGG 0.542824 4:+68061837 MsG0480022090.01.T01:CDS
TCAACAAGGATGTCAAACTA+GGG 0.551047 4:-68061466 None:intergenic
ATTGATTTGGAATGGAAGAC+TGG 0.560840 4:+68062119 MsG0480022090.01.T01:CDS
TTCAAATCTGAGGGATTCAG+TGG 0.571693 4:-68061708 None:intergenic
GGTGTACTTTCCAACAGTCA+TGG 0.589902 4:-68061780 None:intergenic
ACGGTGAAGAAAGAGAAAGA+AGG 0.618741 4:-68061576 None:intergenic
CTACTATGATCAACTCAACA+AGG 0.624192 4:-68061480 None:intergenic
AGAGAAAATTGAGAAGAAGA+CGG 0.629569 4:-68061595 None:intergenic
CAACAAGGATGTCAAACTAG+GGG 0.670486 4:-68061465 None:intergenic
ACCCTCGTTGGGGTTAACGA+TGG 0.671660 4:-68061801 None:intergenic
GGCTGCTGAGAATAAACCTG+AGG 0.681417 4:+68062085 MsG0480022090.01.T01:CDS
GTAAAGGGTAACCCTCGTTG+GGG 0.688463 4:-68061811 None:intergenic
CACCATCGTTAACCCCAACG+AGG 0.704046 4:+68061799 MsG0480022090.01.T01:CDS
AACAAGGATGTCAAACTAGG+GGG 0.710809 4:-68061464 None:intergenic
TTTGGAATGGAAGACTGGTG+TGG 0.728474 4:+68062124 MsG0480022090.01.T01:CDS
ACCATCGTTAACCCCAACGA+GGG 0.730690 4:+68061800 MsG0480022090.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTAATATTTATTATATTCTA+TGG + Chr4:68064089-68064108 MsG0480022090.01.T01:intron 10.0%
!! TAGTAATTTATGATATTAAT+CGG + Chr4:68062941-68062960 MsG0480022090.01.T01:intron 10.0%
!! AAACAAATTAGAATTTAGTA+AGG - Chr4:68063271-68063290 None:intergenic 15.0%
!! ACATAATTCCAAAAAAATAA+AGG + Chr4:68063730-68063749 MsG0480022090.01.T01:intron 15.0%
!! ATATTACAAATTTACCATTA+AGG - Chr4:68064076-68064095 None:intergenic 15.0%
!! CATAATTCCAAAAAAATAAA+GGG + Chr4:68063731-68063750 MsG0480022090.01.T01:intron 15.0%
!!! AGTACATTTTTTTTAATAAC+TGG + Chr4:68062350-68062369 MsG0480022090.01.T01:intron 15.0%
!!! GTACATTTTTTTTAATAACT+GGG + Chr4:68062351-68062370 MsG0480022090.01.T01:intron 15.0%
!!! TATTAATTAATAGGTTGATT+AGG + Chr4:68062446-68062465 MsG0480022090.01.T01:intron 15.0%
!!! TCTATAATATATTTTCAAGT+TGG + Chr4:68062204-68062223 MsG0480022090.01.T01:intron 15.0%
!!! TTATTTTTTTGGAATTATGT+AGG - Chr4:68063730-68063749 None:intergenic 15.0%
!!! TTGATTTTTTTTTCTTTTCT+GGG + Chr4:68061953-68061972 MsG0480022090.01.T01:intron 15.0%
!!! TTTGATTTTTTTTTCTTTTC+TGG + Chr4:68061952-68061971 MsG0480022090.01.T01:intron 15.0%
!! ACTAGATGTATATTAAAGTA+AGG + Chr4:68062285-68062304 MsG0480022090.01.T01:intron 20.0%
!! ATCTGTGTGTATTAATTAAT+AGG + Chr4:68062437-68062456 MsG0480022090.01.T01:intron 20.0%
!! CTAGATGTATATTAAAGTAA+GGG + Chr4:68062286-68062305 MsG0480022090.01.T01:intron 20.0%
!! GTAGTTAAAGTAAAGTAATT+TGG + Chr4:68062759-68062778 MsG0480022090.01.T01:intron 20.0%
!! TTAAAGATTTGTATCCTTAA+TGG + Chr4:68064059-68064078 MsG0480022090.01.T01:intron 20.0%
!!! ATTCAACTGTTTTTGATTAA+TGG + Chr4:68061918-68061937 MsG0480022090.01.T01:intron 20.0%
!!! TTCAACTGTTTTTGATTAAT+GGG + Chr4:68061919-68061938 MsG0480022090.01.T01:intron 20.0%
! AATACACTCCTTACTAAATT+TGG + Chr4:68062318-68062337 MsG0480022090.01.T01:intron 25.0%
! ACAAGCACAATTAATGAAAA+AGG - Chr4:68063302-68063321 None:intergenic 25.0%
! AGAAGAGAAAGATGAAAAAA+GGG - Chr4:68061665-68061684 None:intergenic 25.0%
! AGTTATCAAAATCAAATCAG+TGG - Chr4:68064236-68064255 None:intergenic 25.0%
! ATACACTCCTTACTAAATTT+GGG + Chr4:68062319-68062338 MsG0480022090.01.T01:intron 25.0%
! ATTAATCGGTTAGATAGATA+GGG + Chr4:68062955-68062974 MsG0480022090.01.T01:intron 25.0%
! GTGTATCTTGATTTATTGAA+TGG + Chr4:68064153-68064172 MsG0480022090.01.T01:intron 25.0%
! TAAATTGTTTGATGTTTCCT+TGG + Chr4:68062380-68062399 MsG0480022090.01.T01:intron 25.0%
! TACAGCCAAAAATAATCAAT+GGG + Chr4:68063652-68063671 MsG0480022090.01.T01:intron 25.0%
! TAGAAGAGAAAGATGAAAAA+AGG - Chr4:68061666-68061685 None:intergenic 25.0%
! TATCAAATGAGTGTGATAAT+AGG - Chr4:68064422-68064441 None:intergenic 25.0%
! TATTAATCGGTTAGATAGAT+AGG + Chr4:68062954-68062973 MsG0480022090.01.T01:intron 25.0%
!! AAAGATGAAAAAAGGGTTTT+GGG - Chr4:68061658-68061677 None:intergenic 25.0%
!! TTTAAGTTCAGTAAATCAGA+TGG + Chr4:68063062-68063081 MsG0480022090.01.T01:intron 25.0%
!! TTTTACATTCTGATCAAGAT+CGG - Chr4:68063948-68063967 None:intergenic 25.0%
!!! ACTGATTTGATTTTGATAAC+TGG + Chr4:68064235-68064254 MsG0480022090.01.T01:intron 25.0%
!!! GTACAAACCCTTTATTTTTT+TGG - Chr4:68063741-68063760 None:intergenic 25.0%
!!! TACAACCCATTGATTATTTT+TGG - Chr4:68063660-68063679 None:intergenic 25.0%
!!! TCTCTGGTTTTCAATTAATT+TGG + Chr4:68064474-68064493 MsG0480022090.01.T01:intron 25.0%
!!! TGATTACATGACTTGTTTTT+GGG + Chr4:68062064-68062083 MsG0480022090.01.T01:CDS 25.0%
!!! TTTTTTTCTTTTCTGGGTTT+AGG + Chr4:68061959-68061978 MsG0480022090.01.T01:intron 25.0%
AAAAAACATCTCGTCATGAA+TGG - Chr4:68064609-68064628 None:intergenic 30.0%
AAAGTAGTGAGATTCAATAC+TGG - Chr4:68061891-68061910 None:intergenic 30.0%
AAATCATGGTTATGGAAAAG+TGG - Chr4:68061628-68061647 None:intergenic 30.0%
AAGTAGTGAGATTCAATACT+GGG - Chr4:68061890-68061909 None:intergenic 30.0%
ACTAGATACCAAATTAACTG+AGG - Chr4:68063170-68063189 None:intergenic 30.0%
ACTATGCTATACTAGGATTT+AGG + Chr4:68062651-68062670 MsG0480022090.01.T01:intron 30.0%
ACTCCTTAATCAACCTATAT+AGG - Chr4:68063447-68063466 None:intergenic 30.0%
AGAGAAAATTGAGAAGAAGA+CGG - Chr4:68061598-68061617 None:intergenic 30.0%
AGGTACAAAAGAACCTATAT+AGG + Chr4:68063431-68063450 MsG0480022090.01.T01:intron 30.0%
AGTCACATAGTCAATAATTC+TGG + Chr4:68063010-68063029 MsG0480022090.01.T01:intron 30.0%
ATATTTATGTACCTTACCCA+CGG + Chr4:68064328-68064347 MsG0480022090.01.T01:intron 30.0%
CATGGAAATAACTATCACTA+TGG + Chr4:68061993-68062012 MsG0480022090.01.T01:CDS 30.0%
CTAGATACCAAATTAACTGA+GGG - Chr4:68063169-68063188 None:intergenic 30.0%
GGCATGTTATATTTCAATCA+TGG + Chr4:68064110-68064129 MsG0480022090.01.T01:intron 30.0%
GGGTAAGGTACATAAATATA+TGG - Chr4:68064327-68064346 None:intergenic 30.0%
GTACAGCCAAAAATAATCAA+TGG + Chr4:68063651-68063670 MsG0480022090.01.T01:intron 30.0%
GTAGTAACCCAAATTTAGTA+AGG - Chr4:68062329-68062348 None:intergenic 30.0%
GTGATAATAGGACTTTAACA+AGG - Chr4:68064410-68064429 None:intergenic 30.0%
GTTAAGAGAAATCATGGTTA+TGG - Chr4:68061636-68061655 None:intergenic 30.0%
TAATCATACAGAAATCACCA+AGG - Chr4:68063344-68063363 None:intergenic 30.0%
TAATTATTCTGACGTTTCCT+TGG + Chr4:68063324-68063343 MsG0480022090.01.T01:intron 30.0%
TAGTTTATGATGTCACTAAC+CGG + Chr4:68063849-68063868 MsG0480022090.01.T01:intron 30.0%
TATATCTACTCTTAACTCTC+TGG + Chr4:68064458-68064477 MsG0480022090.01.T01:intron 30.0%
TCTCTATACATCTACCTATT+TGG - Chr4:68064038-68064057 None:intergenic 30.0%
TGGTCTAATGTTGCTAAATA+AGG + Chr4:68063104-68063123 MsG0480022090.01.T01:intron 30.0%
TTAAGGAGTTATGACATGTA+AGG + Chr4:68063458-68063477 MsG0480022090.01.T01:intron 30.0%
TTTACATTCTGATCAAGATC+GGG - Chr4:68063947-68063966 None:intergenic 30.0%
! AAGATTTGAGTTCAAATCTG+AGG - Chr4:68061721-68061740 None:intergenic 30.0%
! ACAAGTAGTTCGAGTAATTT+TGG + Chr4:68063534-68063553 MsG0480022090.01.T01:intron 30.0%
! AGATTTGAGTTCAAATCTGA+GGG - Chr4:68061720-68061739 None:intergenic 30.0%
! CAAGTAGTTCGAGTAATTTT+GGG + Chr4:68063535-68063554 MsG0480022090.01.T01:intron 30.0%
! GAAAGATGAAAAAAGGGTTT+TGG - Chr4:68061659-68061678 None:intergenic 30.0%
! TAGCCTTAAAAAACTATGTG+GGG - Chr4:68062521-68062540 None:intergenic 30.0%
! TGTGATTTGTGTATACTACT+AGG + Chr4:68063376-68063395 MsG0480022090.01.T01:intron 30.0%
! TTCAGATTTGCTCCAAAATA+AGG - Chr4:68064387-68064406 None:intergenic 30.0%
!! CTAGCCTTAAAAAACTATGT+GGG - Chr4:68062522-68062541 None:intergenic 30.0%
!! GAACCTATATAGGTTGATTA+AGG + Chr4:68063441-68063460 MsG0480022090.01.T01:intron 30.0%
!! TGGATTTTTATTTACCCGAA+CGG - Chr4:68061855-68061874 None:intergenic 30.0%
!! TGTATACTACTAGGTTGATT+AGG + Chr4:68063385-68063404 MsG0480022090.01.T01:intron 30.0%
!!! AGATGTTTTTTCTTCGTGAA+AGG + Chr4:68064618-68064637 MsG0480022090.01.T01:CDS 30.0%
!!! AGTAAAACACCTTGCTTTTT+TGG - Chr4:68062839-68062858 None:intergenic 30.0%
!!! ATCACTTTTGTGCCTTATTT+TGG + Chr4:68064372-68064391 MsG0480022090.01.T01:intron 30.0%
!!! GTCTTACATCTTTTTTGATC+TGG + Chr4:68062619-68062638 MsG0480022090.01.T01:intron 30.0%
!!! GTGATTACATGACTTGTTTT+TGG + Chr4:68062063-68062082 MsG0480022090.01.T01:CDS 30.0%
!!! TTTTGGGTTAAGAGAAATCA+TGG - Chr4:68061642-68061661 None:intergenic 30.0%
!!! TTTTTTAAGGCTAGCTGATA+TGG + Chr4:68062528-68062547 MsG0480022090.01.T01:intron 30.0%
AAAAGCAATTCCATGACTGT+TGG + Chr4:68061770-68061789 MsG0480022090.01.T01:CDS 35.0%
AGCCTTAAAAAACTATGTGG+GGG - Chr4:68062520-68062539 None:intergenic 35.0%
ATACTCACATCATAAGCTAC+AGG - Chr4:68062876-68062895 None:intergenic 35.0%
ATACTGGGCAAGAAAAAGTT+TGG - Chr4:68061875-68061894 None:intergenic 35.0%
ATTCAGTGGAAGATGAAAAC+AGG - Chr4:68061697-68061716 None:intergenic 35.0%
ATTGTTGATGTGTGACTGTA+TGG - Chr4:68063915-68063934 None:intergenic 35.0%
CTACTATGATCAACTCAACA+AGG - Chr4:68061483-68061502 None:intergenic 35.0%
CTCTCCTTAAAAAACTGTGT+GGG - Chr4:68063510-68063529 None:intergenic 35.0%
CTTGATTAGATGGCATTCTA+TGG + Chr4:68063995-68064014 MsG0480022090.01.T01:intron 35.0%
GATCTATCCCTCAGTTAATT+TGG + Chr4:68063159-68063178 MsG0480022090.01.T01:intron 35.0%
GTATCTTAGATGAGCTTTAC+TGG + Chr4:68062892-68062911 MsG0480022090.01.T01:intron 35.0%
TAGTAGTTGGCATCTTTGTT+TGG + Chr4:68061497-68061516 MsG0480022090.01.T01:five_prime_UTR 35.0%
TCAACAAGGATGTCAAACTA+GGG - Chr4:68061469-68061488 None:intergenic 35.0%
TCACCTCATATCATAAGCTA+GGG - Chr4:68063701-68063720 None:intergenic 35.0%
TCTCCTTAAAAAACTGTGTG+GGG - Chr4:68063509-68063528 None:intergenic 35.0%
TCTGGATTCTTCGATATTGA+TGG + Chr4:68063028-68063047 MsG0480022090.01.T01:intron 35.0%
TGAAGCTCGTTATTTATGAC+TGG + Chr4:68062916-68062935 MsG0480022090.01.T01:intron 35.0%
TGACCTTTCTTAGCAACATT+GGG - Chr4:68062164-68062183 None:intergenic 35.0%
TTCACCTCATATCATAAGCT+AGG - Chr4:68063702-68063721 None:intergenic 35.0%
TTGACCTTTCTTAGCAACAT+TGG - Chr4:68062165-68062184 None:intergenic 35.0%
! CAGTCACACATCAACAATTT+TGG + Chr4:68063917-68063936 MsG0480022090.01.T01:intron 35.0%
! GGTAACGTTGACTATTGATT+TGG + Chr4:68062106-68062125 MsG0480022090.01.T01:CDS 35.0%
! TTATTGACTATGTGACTGCA+AGG - Chr4:68063007-68063026 None:intergenic 35.0%
! TTTTTCTACAGCAGACTAGA+AGG - Chr4:68062474-68062493 None:intergenic 35.0%
!! ATTGATTTGGAATGGAAGAC+TGG + Chr4:68062119-68062138 MsG0480022090.01.T01:CDS 35.0%
!! CGTTGACTATTGATTTGGAA+TGG + Chr4:68062111-68062130 MsG0480022090.01.T01:CDS 35.0%
!! GCTAGCCTTAAAAAACTATG+TGG - Chr4:68062523-68062542 None:intergenic 35.0%
!! GTGTTTTCTGTTGCTTAATC+TGG + Chr4:68062237-68062256 MsG0480022090.01.T01:intron 35.0%
!! GTTGAGTTGATCATAGTAGT+TGG + Chr4:68061484-68061503 MsG0480022090.01.T01:five_prime_UTR 35.0%
!! TTAGTTCTAGTTCCATTACC+TGG - Chr4:68064562-68064581 None:intergenic 35.0%
!!! ATCCCCCACATAGTTTTTTA+AGG + Chr4:68062515-68062534 MsG0480022090.01.T01:intron 35.0%
!!! CATCTTTTTTGATCTGGTTC+TGG + Chr4:68062625-68062644 MsG0480022090.01.T01:intron 35.0%
!!! CTAGGATTTAGGAGCATTTT+TGG + Chr4:68062662-68062681 MsG0480022090.01.T01:intron 35.0%
!!! TTAGGAGCATTTTTGGTTTG+AGG + Chr4:68062669-68062688 MsG0480022090.01.T01:intron 35.0%
AACAAGGATGTCAAACTAGG+GGG - Chr4:68061467-68061486 None:intergenic 40.0%
AACGGTGATTCTGTGTGAAT+CGG - Chr4:68061837-68061856 None:intergenic 40.0%
AAGGCACAAAAGTGATATCC+AGG - Chr4:68064368-68064387 None:intergenic 40.0%
ACGGAGTTTACTATATTCCC+TGG + Chr4:68064347-68064366 MsG0480022090.01.T01:intron 40.0%
ACGGTGAAGAAAGAGAAAGA+AGG - Chr4:68061579-68061598 None:intergenic 40.0%
ACTAACCGGTTAGATAGTCT+GGG + Chr4:68063863-68063882 MsG0480022090.01.T01:intron 40.0%
AGGCACAAAAGTGATATCCA+GGG - Chr4:68064367-68064386 None:intergenic 40.0%
AGGGAATATAGTAAACTCCG+TGG - Chr4:68064348-68064367 None:intergenic 40.0%
ATCCCCTAGCTTATGATATG+AGG + Chr4:68063695-68063714 MsG0480022090.01.T01:intron 40.0%
ATTCACACAGAATCACCGTT+CGG + Chr4:68061837-68061856 MsG0480022090.01.T01:CDS 40.0%
ATTCTGTGTGAATCGGGTAA+AGG - Chr4:68061830-68061849 None:intergenic 40.0%
CAACAACAACCAAGCCATAT+GGG - Chr4:68063620-68063639 None:intergenic 40.0%
CAACAAGGATGTCAAACTAG+GGG - Chr4:68061468-68061487 None:intergenic 40.0%
CACCTCATATCATAAGCTAG+GGG - Chr4:68063700-68063719 None:intergenic 40.0%
CTCAACAAGGATGTCAAACT+AGG - Chr4:68061470-68061489 None:intergenic 40.0%
CTCCTTAAAAAACTGTGTGG+GGG - Chr4:68063508-68063527 None:intergenic 40.0%
CTGGATGACTATGCTATACT+AGG + Chr4:68062644-68062663 MsG0480022090.01.T01:intron 40.0%
CTGGCATGATTTAGTGCTAT+AGG + Chr4:68062256-68062275 MsG0480022090.01.T01:intron 40.0%
GACCATGTAGTCAACAAATC+TGG - Chr4:68063089-68063108 None:intergenic 40.0%
GCTCTCCTTAAAAAACTGTG+TGG - Chr4:68063511-68063530 None:intergenic 40.0%
GCTGTCTGATCTTGATTAGA+TGG + Chr4:68063985-68064004 MsG0480022090.01.T01:intron 40.0%
GGGAATATAGTAAACTCCGT+GGG - Chr4:68064347-68064366 None:intergenic 40.0%
TATAGTAAACTCCGTGGGTA+AGG - Chr4:68064342-68064361 None:intergenic 40.0%
TATGGAGATCGTGTTCAATC+TGG + Chr4:68062011-68062030 MsG0480022090.01.T01:CDS 40.0%
TCAATAGTCAACGTTACCTC+AGG - Chr4:68062104-68062123 None:intergenic 40.0%
TCACATCATAAGCTACAGGA+TGG - Chr4:68062872-68062891 None:intergenic 40.0%
TCCTTAAAAAACTGTGTGGG+GGG - Chr4:68063507-68063526 None:intergenic 40.0%
TGACTGTATGGATTCAGTGA+TGG - Chr4:68063903-68063922 None:intergenic 40.0%
TGATTTCATTCGCTTGTACC+AGG + Chr4:68064541-68064560 MsG0480022090.01.T01:intron 40.0%
TGCATACAGCCAAAAAAGCA+AGG + Chr4:68062827-68062846 MsG0480022090.01.T01:intron 40.0%
TTCAAATCTGAGGGATTCAG+TGG - Chr4:68061711-68061730 None:intergenic 40.0%
TTCTGTGTGAATCGGGTAAA+GGG - Chr4:68061829-68061848 None:intergenic 40.0%
! AGTTGGCATCTTTGTTTGGT+TGG + Chr4:68061501-68061520 MsG0480022090.01.T01:five_prime_UTR 40.0%
! ATCTGGATTTTCTGCCATAG+TGG + Chr4:68062978-68062997 MsG0480022090.01.T01:intron 40.0%
! CTGGCAGTGATTTCTTTTGT+TGG + Chr4:68064254-68064273 MsG0480022090.01.T01:intron 40.0%
! CTTTTAGTCTGCTGTAGGAA+AGG + Chr4:68063411-68063430 MsG0480022090.01.T01:intron 40.0%
! GAAAGGTACACATGCACAAT+TGG + Chr4:68064635-68064654 MsG0480022090.01.T01:CDS 40.0%
! GGTTTCTTTTAGTCTGCTGT+AGG + Chr4:68063406-68063425 MsG0480022090.01.T01:intron 40.0%
! GTCTATGTCGAGATTTTGTG+AGG + Chr4:68062581-68062600 MsG0480022090.01.T01:intron 40.0%
! GTTTAGGTTACTTCGTCTCA+TGG + Chr4:68061975-68061994 MsG0480022090.01.T01:intron 40.0%
! TTGGTTTGAGGAGTGATTCA+GGG + Chr4:68062681-68062700 MsG0480022090.01.T01:intron 40.0%
!! GATGTCTTCTGAGATGCATT+TGG - Chr4:68061750-68061769 None:intergenic 40.0%
!! GTCCAGATTTGTTGACTACA+TGG + Chr4:68063084-68063103 MsG0480022090.01.T01:intron 40.0%
!! TAATCCTACAGAAAGCACCA+AGG - Chr4:68062400-68062419 None:intergenic 40.0%
!! TTTGGTTTGAGGAGTGATTC+AGG + Chr4:68062680-68062699 MsG0480022090.01.T01:intron 40.0%
AACAACCAAGCCATATGGGT+CGG - Chr4:68063616-68063635 None:intergenic 45.0%
ACGGTGATTCTGTGTGAATC+GGG - Chr4:68061836-68061855 None:intergenic 45.0%
CACTAACCGGTTAGATAGTC+TGG + Chr4:68063862-68063881 MsG0480022090.01.T01:intron 45.0%
CAGATCCCAGACTATCTAAC+CGG - Chr4:68063871-68063890 None:intergenic 45.0%
CAGGGAATCTGGATCCAAAT+AGG + Chr4:68064021-68064040 MsG0480022090.01.T01:intron 45.0%
CATTCGCTTGTACCAGGTAA+TGG + Chr4:68064547-68064566 MsG0480022090.01.T01:intron 45.0%
CGGTTAGATAGATAGGGATC+TGG + Chr4:68062961-68062980 MsG0480022090.01.T01:intron 45.0%
GCAACAACAACCAAGCCATA+TGG - Chr4:68063621-68063640 None:intergenic 45.0%
GGTGTACTTTCCAACAGTCA+TGG - Chr4:68061783-68061802 None:intergenic 45.0%
GTCTCCTACGTGAGATTTAC+TGG + Chr4:68063801-68063820 MsG0480022090.01.T01:intron 45.0%
TCAGCCAGTAAATCTCACGT+AGG - Chr4:68063808-68063827 None:intergenic 45.0%
TGACTGCAAGGATTCCACTA+TGG - Chr4:68062995-68063014 None:intergenic 45.0%
TGGCCCAATGTTGCTAAGAA+AGG + Chr4:68062158-68062177 MsG0480022090.01.T01:CDS 45.0%
TGTATGGATTCAGTGATGGC+AGG - Chr4:68063899-68063918 None:intergenic 45.0%
TTCACACAGAATCACCGTTC+GGG + Chr4:68061838-68061857 MsG0480022090.01.T01:CDS 45.0%
! TTTGGAATGGAAGACTGGTG+TGG + Chr4:68062124-68062143 MsG0480022090.01.T01:CDS 45.0%
!! GTTTCCTTGGTGCTTTCTGT+AGG + Chr4:68062393-68062412 MsG0480022090.01.T01:intron 45.0%
!!! TCCCCCCACACAGTTTTTTA+AGG + Chr4:68063503-68063522 MsG0480022090.01.T01:intron 45.0%
ACCATCGTTAACCCCAACGA+GGG + Chr4:68061800-68061819 MsG0480022090.01.T01:CDS 50.0%
AGATGGCATTCTATGGCAGC+AGG + Chr4:68064002-68064021 MsG0480022090.01.T01:intron 50.0%
AGCAACATTGGGCCAATCCT+TGG - Chr4:68062153-68062172 None:intergenic 50.0%
CAAACTAGGGGGTTCTTACG+TGG - Chr4:68061456-68061475 None:intergenic 50.0%
GATGGCATTCTATGGCAGCA+GGG + Chr4:68064003-68064022 MsG0480022090.01.T01:intron 50.0%
GGCTGCTGAGAATAAACCTG+AGG + Chr4:68062085-68062104 MsG0480022090.01.T01:CDS 50.0%
GGTAAAGGGTAACCCTCGTT+GGG - Chr4:68061815-68061834 None:intergenic 50.0%
GGTACACATGCACAATTGGC+TGG + Chr4:68064639-68064658 MsG0480022090.01.T01:CDS 50.0%
GTAAAGGGTAACCCTCGTTG+GGG - Chr4:68061814-68061833 None:intergenic 50.0%
TTCTATGGCAGCAGGGAATC+TGG + Chr4:68064010-68064029 MsG0480022090.01.T01:intron 50.0%
! CGGTTAGATAGTCTGGGATC+TGG + Chr4:68063869-68063888 MsG0480022090.01.T01:intron 50.0%
! GCATTTGTTGCTGCAGAAGC+TGG + Chr4:68062041-68062060 MsG0480022090.01.T01:CDS 50.0%
ACCCTCGTTGGGGTTAACGA+TGG - Chr4:68061804-68061823 None:intergenic 55.0%
AGCCATATGGGTCGGCTACA+CGG - Chr4:68063608-68063627 None:intergenic 55.0%
CACCATCGTTAACCCCAACG+AGG + Chr4:68061799-68061818 MsG0480022090.01.T01:CDS 55.0%
GGGTAAAGGGTAACCCTCGT+TGG - Chr4:68061816-68061835 None:intergenic 55.0%
TGTAGCCGACCCATATGGCT+TGG + Chr4:68063608-68063627 MsG0480022090.01.T01:intron 55.0%
TTCCGTGTAGCCGACCCATA+TGG + Chr4:68063603-68063622 MsG0480022090.01.T01:intron 55.0%
! CGAGATTTTGTGAGGTCGCG+AGG + Chr4:68062589-68062608 MsG0480022090.01.T01:intron 55.0%
! TTTTGTGAGGTCGCGAGGCT+AGG + Chr4:68062594-68062613 MsG0480022090.01.T01:intron 55.0%
CTGGTGTGGCAGCCAAGGAT+TGG + Chr4:68062138-68062157 MsG0480022090.01.T01:CDS 60.0%
GAAGACTGGTGTGGCAGCCA+AGG + Chr4:68062133-68062152 MsG0480022090.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 68061452 68064685 68061452 ID=MsG0480022090.01;Name=MsG0480022090.01
Chr4 mRNA 68061452 68064685 68061452 ID=MsG0480022090.01.T01;Parent=MsG0480022090.01;Name=MsG0480022090.01.T01;_AED=0.41;_eAED=0.44;_QI=189|1|0.66|1|1|1|3|0|183
Chr4 exon 68061452 68061859 68061452 ID=MsG0480022090.01.T01:exon:18420;Parent=MsG0480022090.01.T01
Chr4 exon 68061981 68062190 68061981 ID=MsG0480022090.01.T01:exon:18421;Parent=MsG0480022090.01.T01
Chr4 exon 68064563 68064685 68064563 ID=MsG0480022090.01.T01:exon:18422;Parent=MsG0480022090.01.T01
Chr4 five_prime_UTR 68061452 68061640 68061452 ID=MsG0480022090.01.T01:five_prime_utr;Parent=MsG0480022090.01.T01
Chr4 CDS 68061641 68061859 68061641 ID=MsG0480022090.01.T01:cds;Parent=MsG0480022090.01.T01
Chr4 CDS 68061981 68062190 68061981 ID=MsG0480022090.01.T01:cds;Parent=MsG0480022090.01.T01
Chr4 CDS 68064563 68064685 68064563 ID=MsG0480022090.01.T01:cds;Parent=MsG0480022090.01.T01
Gene Sequence

>MsG0480022090.01.T01

ATGATTTCTCTTAACCCAAAACCCTTTTTTCATCTTTCTCTTCTACTTCTCATCCTGTTTTCATCTTCCACTGAATCCCTCAGATTTGAACTCAAATCTTCTTTAACCAAATGCATCTCAGAAGACATCAAAAGCAATTCCATGACTGTTGGAAAGTACACCATCGTTAACCCCAACGAGGGTTACCCTTTACCCGATTCACACAGAATCACCGTTCGGGTTACTTCGTCTCATGGAAATAACTATCACTATGGAGATCGTGTTCAATCTGGTCAGTTTGCATTTGTTGCTGCAGAAGCTGGTGATTACATGACTTGTTTTTGGGCTGCTGAGAATAAACCTGAGGTAACGTTGACTATTGATTTGGAATGGAAGACTGGTGTGGCAGCCAAGGATTGGCCCAATGTTGCTAAGAAAGGTCAAGTTGATGTAATGGAACTAGAACTAAAGAAGTTACAAGATACTGTTACTTCCATTCATGACGAGATGTTTTTTCTTCGTGAAAGGTACACATGCACAATTGGCTGGAAAATCTTTATGCAATACATATGA
Protein sequence

>MsG0480022090.01.T01

MISLNPKPFFHLSLLLLILFSSSTESLRFELKSSLTKCISEDIKSNSMTVGKYTIVNPNEGYPLPDSHRITVRVTSSHGNNYHYGDRVQSGQFAFVAAEAGDYMTCFWAAENKPEVTLTIDLEWKTGVAAKDWPNVAKKGQVDVMELELKKLQDTVTSIHDEMFFLRERYTCTIGWKIFMQYI*