AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005231.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005231.01.T01 MTR_1g095620 77.481 262 13 1 12 273 1 216 2.83e-141 396
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005231.01.T01 AT5G52980 51.362 257 79 1 12 268 1 211 1.03e-78 238
MsG0180005231.01.T01 AT5G52980 50.000 210 59 1 12 221 1 164 3.87e-61 192

Find 64 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCTTACAAGGATCAATT+AGG 0.294940 1:-88900812 MsG0180005231.01.T01:CDS
AAGCTATTCTCTAAAGCTTA+TGG 0.295378 1:-88901164 MsG0180005231.01.T01:CDS
TACAAGGATCAATTAGGATT+TGG 0.302915 1:-88900806 MsG0180005231.01.T01:intron
AATGCAGCTGGAGGAATTCT+TGG 0.319711 1:-88898796 MsG0180005231.01.T01:CDS
AACTTTCATGTAGACTCTTT+TGG 0.327754 1:-88900953 MsG0180005231.01.T01:CDS
CGAATTGACCCAACTCTTAT+CGG 0.345328 1:-88901515 MsG0180005231.01.T01:CDS
GGTTCTGCAACATCTTCCTT+TGG 0.350261 1:+88900839 None:intergenic
CGATAAGAGTTGGGTCAATT+CGG 0.354345 1:+88901516 None:intergenic
ATGAGCCACTCCGTGCTATC+TGG 0.355787 1:-88901564 MsG0180005231.01.T01:CDS
ATGTTTACTGGCTATCTTGT+TGG 0.379056 1:-88899377 MsG0180005231.01.T01:CDS
ATCCAGCACCCATTCCATTC+GGG 0.394845 1:-88901677 MsG0180005231.01.T01:CDS
AGTGGTGTCGGTGGAGGCTT+CGG 0.403863 1:+88901645 None:intergenic
GATAAGAGTTGGGTCAATTC+GGG 0.420691 1:+88901517 None:intergenic
TGGAGGAATTCTTGGGTTGG+TGG 0.424210 1:-88898788 MsG0180005231.01.T01:CDS
GGAGGAATTCTTGGGTTGGT+GGG 0.446938 1:-88898787 MsG0180005231.01.T01:CDS
CGGAATTTCTAATAATGAAA+AGG 0.448076 1:+88898743 None:intergenic
TATCCAGCACCCATTCCATT+CGG 0.456466 1:-88901678 MsG0180005231.01.T01:CDS
GCTCATACGGTACGTCGGAT+TGG 0.464698 1:+88901581 None:intergenic
CTATGCTTTCAGAATGCAGC+TGG 0.465602 1:-88898808 MsG0180005231.01.T01:intron
AGCTGGAGGAATTCTTGGGT+TGG 0.470243 1:-88898791 MsG0180005231.01.T01:CDS
ATGCAGCTGGAGGAATTCTT+GGG 0.477407 1:-88898795 MsG0180005231.01.T01:CDS
GAGAAAATCCCGAATGGAAT+GGG 0.478391 1:+88901669 None:intergenic
AAAACATGTCGAATCGAGTC+GGG 0.481485 1:-88901708 MsG0180005231.01.T01:CDS
ATCCCGAATGGAATGGGTGC+TGG 0.489046 1:+88901675 None:intergenic
GGAGAGAGTGGTGTCGGTGG+AGG 0.490128 1:+88901639 None:intergenic
AGAACCATTCTCTTCTTACA+AGG 0.500737 1:-88900822 MsG0180005231.01.T01:CDS
CCACCTTTGATCATGTCTAC+AGG 0.501142 1:+88898644 None:intergenic
TGATCCTTGTAAGAAGAGAA+TGG 0.506348 1:+88900818 None:intergenic
AGCTGGTACCCGATAAGAGT+TGG 0.519174 1:+88901506 None:intergenic
CAGCCCTAAACCCAGAGAAA+AGG 0.527992 1:-88901473 MsG0180005231.01.T01:intron
TTGGGTCAATTCGGGTCTAG+TGG 0.528607 1:+88901525 None:intergenic
AGAGAAAATCCCGAATGGAA+TGG 0.536672 1:+88901668 None:intergenic
GCTGGTACCCGATAAGAGTT+GGG 0.537393 1:+88901507 None:intergenic
GTCAATTCGGGTCTAGTGGA+TGG 0.540988 1:+88901529 None:intergenic
GAATTGACCCAACTCTTATC+GGG 0.544516 1:-88901514 MsG0180005231.01.T01:CDS
TCGGAGTTCGGCGGAGAGAG+TGG 0.547801 1:+88901627 None:intergenic
TGTGAAGAGTGAAGAATTGA+AGG 0.556219 1:-88900930 MsG0180005231.01.T01:CDS
GTTTGATCACAGTCCTGCCA+TGG 0.557538 1:-88899333 MsG0180005231.01.T01:intron
AGTTATAGATAACTTAGAAG+TGG 0.563972 1:-88898543 MsG0180005231.01.T01:three_prime_UTR
TAAGCAACTGTGAGACTGCA+CGG 0.566524 1:+88901087 None:intergenic
TTCGGCGGAGAGAGTGGTGT+CGG 0.576822 1:+88901633 None:intergenic
TGCTTTCAGAATGCAGCTGG+AGG 0.584876 1:-88898805 MsG0180005231.01.T01:intron
TGTGAAGGATATTGCACCAA+AGG 0.589058 1:-88900855 MsG0180005231.01.T01:CDS
ATAGCACGGAGTGGCTCATA+CGG 0.591908 1:+88901568 None:intergenic
AACTGTGAGACTGCACGGGA+GGG 0.593767 1:+88901092 None:intergenic
GTCGGATTGGATGAGCAGAT+CGG 0.594127 1:+88901594 None:intergenic
GGCGGAGAGAGTGGTGTCGG+TGG 0.594161 1:+88901636 None:intergenic
CAGAGGCGATCCAGATAGCA+CGG 0.602979 1:+88901554 None:intergenic
CAACTGTGAGACTGCACGGG+AGG 0.609512 1:+88901091 None:intergenic
AGAGTGAAGAATTGAAGGCA+AGG 0.610572 1:-88900925 MsG0180005231.01.T01:CDS
AGGGCTGGAGAAGATGAAGC+TGG 0.611079 1:+88901489 None:intergenic
GAAAACATGTCGAATCGAGT+CGG 0.618986 1:-88901709 MsG0180005231.01.T01:CDS
TCATTCATGCGTGAAGACCA+TGG 0.620089 1:+88901766 None:intergenic
AAGCAACTGTGAGACTGCAC+GGG 0.623346 1:+88901088 None:intergenic
AAGGAAAGTCTCAACAAGCA+TGG 0.624200 1:+88898762 None:intergenic
AAACCTGTAGACATGATCAA+AGG 0.628970 1:-88898647 MsG0180005231.01.T01:exon
GGGTCTAGTGGATGGATCAG+AGG 0.629519 1:+88901537 None:intergenic
AGCATATCAAGAGCTTGTGA+AGG 0.635147 1:-88900870 MsG0180005231.01.T01:CDS
CCTGTAGACATGATCAAAGG+TGG 0.651197 1:-88898644 MsG0180005231.01.T01:exon
TTCGGAGAGAAAATCCCGAA+TGG 0.655968 1:+88901663 None:intergenic
AATTGGAAGATATAGCAGAG+AGG 0.675662 1:-88900895 MsG0180005231.01.T01:CDS
GATGTGGAAAATGATGATCT+CGG 0.716737 1:+88898682 None:intergenic
GCGATCCAGATAGCACGGAG+TGG 0.736192 1:+88901559 None:intergenic
GAGTGGCTCATACGGTACGT+CGG 0.746547 1:+88901576 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGAATTTAATTACAAAAAA+AGG - Chr1:88898495-88898514 MsG0180005231.01.T01:three_prime_UTR 10.0%
!!! TTATTTTAGCTTATAAAAAA+AGG + Chr1:88901010-88901029 None:intergenic 10.0%
!!! TTTCTTTTTTAATTTTTTTC+AGG - Chr1:88900872-88900891 MsG0180005231.01.T01:CDS 10.0%
!! AATTATAACAGTAATATAGA+GGG + Chr1:88899268-88899287 None:intergenic 15.0%
!! ATAATAGAGAGAATATTAAA+GGG + Chr1:88900710-88900729 None:intergenic 15.0%
!! TATAATAGAGAGAATATTAA+AGG + Chr1:88900711-88900730 None:intergenic 15.0%
!! TGTTCATTTATTTAGTTATT+TGG - Chr1:88899586-88899605 MsG0180005231.01.T01:intron 15.0%
!!! ACACTATAATTTTGTATTAT+TGG + Chr1:88901698-88901717 None:intergenic 15.0%
!!! TTATCTTTTGTTATAGAAAT+AGG + Chr1:88899063-88899082 None:intergenic 15.0%
!! AAAATACCTCATAACTTAAT+AGG + Chr1:88899104-88899123 None:intergenic 20.0%
!! AAATATCAAAAGTGAACTAA+AGG + Chr1:88900532-88900551 None:intergenic 20.0%
!! AATCAAAATAAACTCTGTTA+CGG + Chr1:88900198-88900217 None:intergenic 20.0%
!! ATAGATAAGAAAGGAAAAAA+AGG + Chr1:88901467-88901486 None:intergenic 20.0%
!! CAATTATAACAGTAATATAG+AGG + Chr1:88899269-88899288 None:intergenic 20.0%
!! TATGAGAATAATGAAAAACA+AGG + Chr1:88898863-88898882 None:intergenic 20.0%
!! TGTAATTCTATGACATAATT+TGG + Chr1:88901068-88901087 None:intergenic 20.0%
!! TTTATGTTAGTAGTATATGT+TGG - Chr1:88900384-88900403 MsG0180005231.01.T01:intron 20.0%
!!! AAAGTGCTTAATAATTAAGT+TGG + Chr1:88899004-88899023 None:intergenic 20.0%
!!! ATATAACAAGTACTGTAAAT+CGG + Chr1:88900449-88900468 None:intergenic 20.0%
!!! TATCTATAAAGTTTTTTGTG+TGG - Chr1:88899547-88899566 MsG0180005231.01.T01:intron 20.0%
!!! TTCTCAATTGTAGTTTTATT+GGG - Chr1:88900155-88900174 MsG0180005231.01.T01:intron 20.0%
!!! TTGGATATTTTTTTGTTCTT+CGG + Chr1:88901133-88901152 None:intergenic 20.0%
! AAACGACAATAACATAAAGA+GGG + Chr1:88901397-88901416 None:intergenic 25.0%
! AAACTACAATTGAGAACATA+CGG + Chr1:88900151-88900170 None:intergenic 25.0%
! AATTACAAACCAACTTAAGA+GGG - Chr1:88901081-88901100 MsG0180005231.01.T01:intron 25.0%
! AGTTATAGATAACTTAGAAG+TGG - Chr1:88901748-88901767 MsG0180005231.01.T01:CDS 25.0%
! ATCTAGATAGCAACAAAAAA+AGG - Chr1:88901571-88901590 MsG0180005231.01.T01:CDS 25.0%
! ATGTAATTAGATATTCGATG+TGG - Chr1:88898943-88898962 MsG0180005231.01.T01:intron 25.0%
! CGGAATTTCTAATAATGAAA+AGG + Chr1:88901551-88901570 None:intergenic 25.0%
! CTCAAACAAATCAATACAAA+AGG - Chr1:88899241-88899260 MsG0180005231.01.T01:intron 25.0%
! GGAAAAGATATGATTGATTA+TGG - Chr1:88898964-88898983 MsG0180005231.01.T01:intron 25.0%
! GTAAAACATAGTACATTATC+CGG + Chr1:88900565-88900584 None:intergenic 25.0%
! GTAAATGAAGGATAGATAAA+TGG + Chr1:88899970-88899989 None:intergenic 25.0%
! GTTTATAGAAGTTGTTAGTA+TGG - Chr1:88899919-88899938 MsG0180005231.01.T01:intron 25.0%
! TAAACGACAATAACATAAAG+AGG + Chr1:88901398-88901417 None:intergenic 25.0%
! TCATAATGAACAGTAAATGA+AGG + Chr1:88899982-88900001 None:intergenic 25.0%
! TCATTTAGATTGACTATTGT+TGG - Chr1:88899696-88899715 MsG0180005231.01.T01:intron 25.0%
! TTGATATTTAAACCTTTCAC+CGG - Chr1:88900543-88900562 MsG0180005231.01.T01:intron 25.0%
!! AAAAGAAAAAACATGCCTTT+TGG - Chr1:88900279-88900298 MsG0180005231.01.T01:intron 25.0%
!! ACAGTGTTTTACAATTACAA+TGG + Chr1:88901724-88901743 None:intergenic 25.0%
!! ATGATAGTTTGGACTATTTT+TGG + Chr1:88901350-88901369 None:intergenic 25.0%
!! TTCAGTGTTTCTGAAAATTT+CGG + Chr1:88901172-88901191 None:intergenic 25.0%
!!! GTTCTCAATTGTAGTTTTAT+TGG - Chr1:88900154-88900173 MsG0180005231.01.T01:intron 25.0%
!!! TACTTGTTATATATAGCAAC+AGG - Chr1:88900457-88900476 MsG0180005231.01.T01:intron 25.0%
!!! TTAGATATGTTGTTTCATCT+TGG + Chr1:88901216-88901235 None:intergenic 25.0%
!!! TTTTTTGTTGCTATCTAGAT+CGG + Chr1:88901571-88901590 None:intergenic 25.0%
AAAATACTTCAGAGATATGC+AGG + Chr1:88900845-88900864 None:intergenic 30.0%
AACGACAATAACATAAAGAG+GGG + Chr1:88901396-88901415 None:intergenic 30.0%
ACTTCTATAAACAAAACCTC+TGG + Chr1:88899911-88899930 None:intergenic 30.0%
AGCAAAATTGTAGTTGTGAT+TGG + Chr1:88900508-88900527 None:intergenic 30.0%
ATGTTAGTAGTATATGTTGG+TGG - Chr1:88900387-88900406 MsG0180005231.01.T01:intron 30.0%
ATTACAGTGTATTGCATACT+TGG + Chr1:88899868-88899887 None:intergenic 30.0%
CGGCATATAGTTTATTAAGT+TGG + Chr1:88901152-88901171 None:intergenic 30.0%
CTACATGGTTTATGATAGTT+TGG + Chr1:88901361-88901380 None:intergenic 30.0%
CTGTTTCCTATTAAGTTATG+AGG - Chr1:88899095-88899114 MsG0180005231.01.T01:intron 30.0%
CTTGATTGAACTTAGTAACA+TGG - Chr1:88900231-88900250 MsG0180005231.01.T01:intron 30.0%
GAAGGCAAGGTTAAAAAAAT+TGG - Chr1:88899379-88899398 MsG0180005231.01.T01:CDS 30.0%
GAATTACAAACCAACTTAAG+AGG - Chr1:88901080-88901099 MsG0180005231.01.T01:intron 30.0%
GATAGATAAATGGTCAAATC+CGG + Chr1:88899960-88899979 None:intergenic 30.0%
GTTTCATTTGTCTACTATGA+GGG - Chr1:88899517-88899536 MsG0180005231.01.T01:intron 30.0%
TACAAGGATCAATTAGGATT+TGG - Chr1:88899485-88899504 MsG0180005231.01.T01:intron 30.0%
TCTTCTTACAAGGATCAATT+AGG - Chr1:88899479-88899498 MsG0180005231.01.T01:intron 30.0%
TGTGGAAAAGATTGTAAAAG+TGG + Chr1:88901284-88901303 None:intergenic 30.0%
TGTTTCATTTGTCTACTATG+AGG - Chr1:88899516-88899535 MsG0180005231.01.T01:intron 30.0%
TTTCAGAAATGGACAAGAAA+TGG - Chr1:88900082-88900101 MsG0180005231.01.T01:intron 30.0%
! AACTTTCATGTAGACTCTTT+TGG - Chr1:88899338-88899357 MsG0180005231.01.T01:CDS 30.0%
! ACAGATTTTAGACAATACCA+TGG + Chr1:88900978-88900997 None:intergenic 30.0%
! CTTGAGATTTTTGTGTGTTT+TGG - Chr1:88899303-88899322 MsG0180005231.01.T01:intron 30.0%
! TCTGAAAGCATAGATAAGAA+AGG + Chr1:88901476-88901495 None:intergenic 30.0%
!! AAGCTATTCTCTAAAGCTTA+TGG - Chr1:88899127-88899146 MsG0180005231.01.T01:intron 30.0%
!! CAAAAAAAGGAGTATTTCCA+TGG - Chr1:88898508-88898527 MsG0180005231.01.T01:three_prime_UTR 30.0%
!! TTAAGTTGGTTATCCAAACA+AGG + Chr1:88898990-88899009 None:intergenic 30.0%
!!! ATAGTTTGGACTATTTTTGG+TGG + Chr1:88901347-88901366 None:intergenic 30.0%
!!! GTTTTGGTTACAATGTTTAC+TGG - Chr1:88900902-88900921 MsG0180005231.01.T01:CDS 30.0%
!!! TAGCCATTTTTTGTTTCTGA+TGG + Chr1:88898560-88898579 None:intergenic 30.0%
!!! TTTTTCAGGTTTGCATGTTT+TGG - Chr1:88900886-88900905 MsG0180005231.01.T01:CDS 30.0%
AAACCTGTAGACATGATCAA+AGG - Chr1:88901644-88901663 MsG0180005231.01.T01:CDS 35.0%
AATTGGAAGATATAGCAGAG+AGG - Chr1:88899396-88899415 MsG0180005231.01.T01:CDS 35.0%
AGAAATGGACAAGAAATGGA+GGG - Chr1:88900086-88900105 MsG0180005231.01.T01:intron 35.0%
AGAACCATTCTCTTCTTACA+AGG - Chr1:88899469-88899488 MsG0180005231.01.T01:intron 35.0%
AGTTAGGCAATTTCTTGTGT+TGG - Chr1:88900636-88900655 MsG0180005231.01.T01:intron 35.0%
ATAGGCACAAGTGATAGATT+AGG + Chr1:88900059-88900078 None:intergenic 35.0%
ATAGTACATTATCCGGTGAA+AGG + Chr1:88900558-88900577 None:intergenic 35.0%
ATGCACCTAGAAAATAGAGA+GGG + Chr1:88900773-88900792 None:intergenic 35.0%
CAAAACTGAAAGCTTAGAGT+TGG + Chr1:88901261-88901280 None:intergenic 35.0%
CAAAATAAACTCTGTTACGG+TGG + Chr1:88900195-88900214 None:intergenic 35.0%
CCACCATCAGAAACAAAAAA+TGG - Chr1:88898554-88898573 MsG0180005231.01.T01:three_prime_UTR 35.0%
CTTGGTTGTTGTAGTATTGT+TGG - Chr1:88899774-88899793 MsG0180005231.01.T01:intron 35.0%
GATGTGGAAAATGATGATCT+CGG + Chr1:88901612-88901631 None:intergenic 35.0%
GTAGATAAATTGAGCAAACC+TGG - Chr1:88900108-88900127 MsG0180005231.01.T01:intron 35.0%
GTGAGGTCCAGTTATTAAAA+TGG - Chr1:88900028-88900047 MsG0180005231.01.T01:intron 35.0%
GTTGTAGTATTGTTGGATGT+TGG - Chr1:88899781-88899800 MsG0180005231.01.T01:intron 35.0%
TAGACTAGAGAAACTACATC+TGG + Chr1:88900804-88900823 None:intergenic 35.0%
TAGGCACAAGTGATAGATTA+GGG + Chr1:88900058-88900077 None:intergenic 35.0%
TCTCTAGTCTATTATAGACG+CGG - Chr1:88900813-88900832 MsG0180005231.01.T01:CDS 35.0%
TGATCCTTGTAAGAAGAGAA+TGG + Chr1:88899476-88899495 None:intergenic 35.0%
TGTGAAGAGTGAAGAATTGA+AGG - Chr1:88899361-88899380 MsG0180005231.01.T01:CDS 35.0%
TTTGTTTGTACTTTGTCCAG+AGG - Chr1:88899892-88899911 MsG0180005231.01.T01:intron 35.0%
! ATGTTTACTGGCTATCTTGT+TGG - Chr1:88900914-88900933 MsG0180005231.01.T01:CDS 35.0%
! ATTTTAGACAATACCATGGC+AGG + Chr1:88900974-88900993 None:intergenic 35.0%
! CATTTGAGTATCTGAAGCTT+TGG - Chr1:88899660-88899679 MsG0180005231.01.T01:intron 35.0%
! TACAATCTTTTCCACATCCA+AGG - Chr1:88901288-88901307 MsG0180005231.01.T01:intron 35.0%
! TGTCCATTTCTGAAAGAGAT+AGG + Chr1:88900077-88900096 None:intergenic 35.0%
! TTTTCCCCTCTCTATTTTCT+AGG - Chr1:88900765-88900784 MsG0180005231.01.T01:intron 35.0%
!! TGATTGATTATGGCCTTGTT+TGG - Chr1:88898974-88898993 MsG0180005231.01.T01:intron 35.0%
!! TTGAACACCTTTTGATTACG+AGG - Chr1:88901321-88901340 MsG0180005231.01.T01:intron 35.0%
!!! ACATGCCTTTTGGTTTGAAA+AGG - Chr1:88900289-88900308 MsG0180005231.01.T01:intron 35.0%
!!! ATGCCTTTTGGTTTGAAAAG+GGG - Chr1:88900291-88900310 MsG0180005231.01.T01:intron 35.0%
!!! CATGCCTTTTGGTTTGAAAA+GGG - Chr1:88900290-88900309 MsG0180005231.01.T01:intron 35.0%
!!! CCATTTTTTGTTTCTGATGG+TGG + Chr1:88898557-88898576 None:intergenic 35.0%
AAAACATGTCGAATCGAGTC+GGG - Chr1:88898583-88898602 MsG0180005231.01.T01:three_prime_UTR 40.0%
AAGGAAAGTCTCAACAAGCA+TGG + Chr1:88901532-88901551 None:intergenic 40.0%
ACACAAGTGGAAGATTAGTG+AGG - Chr1:88900011-88900030 MsG0180005231.01.T01:intron 40.0%
ACTATTGTTGGACAGAACAC+TGG - Chr1:88899708-88899727 MsG0180005231.01.T01:intron 40.0%
AGAGAAAATCCCGAATGGAA+TGG + Chr1:88898626-88898645 None:intergenic 40.0%
AGAGTGAAGAATTGAAGGCA+AGG - Chr1:88899366-88899385 MsG0180005231.01.T01:CDS 40.0%
AGCATATCAAGAGCTTGTGA+AGG - Chr1:88899421-88899440 MsG0180005231.01.T01:intron 40.0%
AGGCACAAGTGATAGATTAG+GGG + Chr1:88900057-88900076 None:intergenic 40.0%
ATGAACAGAGAAAGACGTAC+AGG + Chr1:88899573-88899592 None:intergenic 40.0%
ATGAGAGAGATAGACACAAG+TGG - Chr1:88899998-88900017 MsG0180005231.01.T01:intron 40.0%
CAAGGAATTGAAAGTAGCGT+TGG + Chr1:88898845-88898864 None:intergenic 40.0%
CAGAAATGGACAAGAAATGG+AGG - Chr1:88900085-88900104 MsG0180005231.01.T01:intron 40.0%
CATGCACCTAGAAAATAGAG+AGG + Chr1:88900774-88900793 None:intergenic 40.0%
CGAATTGACCCAACTCTTAT+CGG - Chr1:88898776-88898795 MsG0180005231.01.T01:CDS 40.0%
CGATAAGAGTTGGGTCAATT+CGG + Chr1:88898778-88898797 None:intergenic 40.0%
CTGTTGTCAGTGAAGAAGTT+AGG - Chr1:88900620-88900639 MsG0180005231.01.T01:intron 40.0%
GAAAACATGTCGAATCGAGT+CGG - Chr1:88898582-88898601 MsG0180005231.01.T01:three_prime_UTR 40.0%
GAATTGACCCAACTCTTATC+GGG - Chr1:88898777-88898796 MsG0180005231.01.T01:CDS 40.0%
GAGAAAATCCCGAATGGAAT+GGG + Chr1:88898625-88898644 None:intergenic 40.0%
GATAAGAGTTGGGTCAATTC+GGG + Chr1:88898777-88898796 None:intergenic 40.0%
GTGCCTATCTCTTTCAGAAA+TGG - Chr1:88900071-88900090 MsG0180005231.01.T01:intron 40.0%
TGAAAAGGGGACATTGTGTT+GGG - Chr1:88900304-88900323 MsG0180005231.01.T01:intron 40.0%
TGCACCTAGAAAATAGAGAG+GGG + Chr1:88900772-88900791 None:intergenic 40.0%
TGTGAAGGATATTGCACCAA+AGG - Chr1:88899436-88899455 MsG0180005231.01.T01:intron 40.0%
TGTTAGTATGGCTGCAAAGT+TGG - Chr1:88899931-88899950 MsG0180005231.01.T01:intron 40.0%
TGTTCAATACACAGTTGCCT+TGG + Chr1:88901308-88901327 None:intergenic 40.0%
TTGAAAAGGGGACATTGTGT+TGG - Chr1:88900303-88900322 MsG0180005231.01.T01:intron 40.0%
! CTACAGGTTTTTCTTGAGAC+TGG + Chr1:88901634-88901653 None:intergenic 40.0%
! GTTTTTCTTGAGACTGGATG+TGG + Chr1:88901628-88901647 None:intergenic 40.0%
! TGTCCCCTTTTCAAACCAAA+AGG + Chr1:88900297-88900316 None:intergenic 40.0%
! TGTTTCCCTAGGTAGTTTGT+TGG + Chr1:88901110-88901129 None:intergenic 40.0%
!! GGGGCATCCATTTTAATAAC+TGG + Chr1:88900038-88900057 None:intergenic 40.0%
!! GTATATGTTGGTGGTACTAG+TGG - Chr1:88900396-88900415 MsG0180005231.01.T01:intron 40.0%
!! GTTTCCCTAGGTAGTTTGTT+GGG + Chr1:88901109-88901128 None:intergenic 40.0%
!! TTTCCCTAGGTAGTTTGTTG+GGG + Chr1:88901108-88901127 None:intergenic 40.0%
AATGCAGCTGGAGGAATTCT+TGG - Chr1:88901495-88901514 MsG0180005231.01.T01:CDS 45.0%
ATACACAGTTGCCTTGGATG+TGG + Chr1:88901302-88901321 None:intergenic 45.0%
ATCAACACTCGCAATCACTG+AGG + Chr1:88900670-88900689 None:intergenic 45.0%
ATGCAGCTGGAGGAATTCTT+GGG - Chr1:88901496-88901515 MsG0180005231.01.T01:CDS 45.0%
CCACCTTTGATCATGTCTAC+AGG + Chr1:88901650-88901669 None:intergenic 45.0%
CCTGTAGACATGATCAAAGG+TGG - Chr1:88901647-88901666 MsG0180005231.01.T01:CDS 45.0%
CTATGCTTTCAGAATGCAGC+TGG - Chr1:88901483-88901502 MsG0180005231.01.T01:CDS 45.0%
GGGATGCTTAGCAATCTACA+TGG + Chr1:88901376-88901395 None:intergenic 45.0%
GGTTCTGCAACATCTTCCTT+TGG + Chr1:88899455-88899474 None:intergenic 45.0%
TAAGCAACTGTGAGACTGCA+CGG + Chr1:88899207-88899226 None:intergenic 45.0%
TATCCAGCACCCATTCCATT+CGG - Chr1:88898613-88898632 MsG0180005231.01.T01:three_prime_UTR 45.0%
TCATTCATGCGTGAAGACCA+TGG + Chr1:88898528-88898547 None:intergenic 45.0%
TGGCACTTACTGTATGACTC+TGG - Chr1:88900331-88900350 MsG0180005231.01.T01:intron 45.0%
TTCGGAGAGAAAATCCCGAA+TGG + Chr1:88898631-88898650 None:intergenic 45.0%
! AAGCTTTATCCCACCAAGTG+GGG + Chr1:88899757-88899776 None:intergenic 45.0%
! AGCTTAGAGTTGGCTCACTA+CGG + Chr1:88901251-88901270 None:intergenic 45.0%
! CAAGCTTTATCCCACCAAGT+GGG + Chr1:88899758-88899777 None:intergenic 45.0%
! GATTGCAATTTTGCGAGTCC+AGG + Chr1:88900129-88900148 None:intergenic 45.0%
! GGACCTTTTCTCTGGGTTTA+GGG + Chr1:88898824-88898843 None:intergenic 45.0%
! TGGACCTTTTCTCTGGGTTT+AGG + Chr1:88898825-88898844 None:intergenic 45.0%
! TTGGTGGCCTCGTAATCAAA+AGG + Chr1:88901331-88901350 None:intergenic 45.0%
!! GCAGATCGGAGCATTTTTGT+CGG + Chr1:88898686-88898705 None:intergenic 45.0%
!! GGAGCATTTTTGTCGGAGTT+CGG + Chr1:88898679-88898698 None:intergenic 45.0%
!! TAGCGTTGGACCTTTTCTCT+GGG + Chr1:88898831-88898850 None:intergenic 45.0%
!! TTGTTCTTCGGTGTTTCCCT+AGG + Chr1:88901121-88901140 None:intergenic 45.0%
AAGCAACTGTGAGACTGCAC+GGG + Chr1:88899206-88899225 None:intergenic 50.0%
AGCTGGAGGAATTCTTGGGT+TGG - Chr1:88901500-88901519 MsG0180005231.01.T01:CDS 50.0%
AGCTGGTACCCGATAAGAGT+TGG + Chr1:88898788-88898807 None:intergenic 50.0%
ATAGCACGGAGTGGCTCATA+CGG + Chr1:88898726-88898745 None:intergenic 50.0%
ATCCAGCACCCATTCCATTC+GGG - Chr1:88898614-88898633 MsG0180005231.01.T01:three_prime_UTR 50.0%
ATGGCTGCAAAGTTGGAGTC+CGG - Chr1:88899938-88899957 MsG0180005231.01.T01:intron 50.0%
CAGCCCTAAACCCAGAGAAA+AGG - Chr1:88898818-88898837 MsG0180005231.01.T01:intron 50.0%
CTTGGCGCAGCAACAAAGTA+AGG + Chr1:88899850-88899869 None:intergenic 50.0%
GCTGGTACCCGATAAGAGTT+GGG + Chr1:88898787-88898806 None:intergenic 50.0%
GGACCCCAACAAACTACCTA+GGG - Chr1:88901102-88901121 MsG0180005231.01.T01:CDS 50.0%
GTCAATTCGGGTCTAGTGGA+TGG + Chr1:88898765-88898784 None:intergenic 50.0%
GTTTGATCACAGTCCTGCCA+TGG - Chr1:88900958-88900977 MsG0180005231.01.T01:CDS 50.0%
TGCTTTCAGAATGCAGCTGG+AGG - Chr1:88901486-88901505 MsG0180005231.01.T01:CDS 50.0%
TGGCTCACTACGGGATTATC+TGG + Chr1:88901241-88901260 None:intergenic 50.0%
TGTTGGGGTCCCTCTTAAGT+TGG + Chr1:88901093-88901112 None:intergenic 50.0%
TTCATGTAACCGACCCCACT+TGG - Chr1:88899741-88899760 MsG0180005231.01.T01:intron 50.0%
TTGGGTCAATTCGGGTCTAG+TGG + Chr1:88898769-88898788 None:intergenic 50.0%
TTTATCCCACCAAGTGGGGT+CGG + Chr1:88899753-88899772 None:intergenic 50.0%
! CCAAGCTTTATCCCACCAAG+TGG + Chr1:88899759-88899778 None:intergenic 50.0%
! CCACTTGGTGGGATAAAGCT+TGG - Chr1:88899756-88899775 MsG0180005231.01.T01:intron 50.0%
! GCATTTTTGTCGGAGTTCGG+CGG + Chr1:88898676-88898695 None:intergenic 50.0%
! GCTTAGAGTTGGCTCACTAC+GGG + Chr1:88901250-88901269 None:intergenic 50.0%
! GTCGGATTGGATGAGCAGAT+CGG + Chr1:88898700-88898719 None:intergenic 50.0%
!! CTTTTCTCTGGGTTTAGGGC+TGG + Chr1:88898820-88898839 None:intergenic 50.0%
!! GGAGGAATTCTTGGGTTGGT+GGG - Chr1:88901504-88901523 MsG0180005231.01.T01:CDS 50.0%
!! GTAGCGTTGGACCTTTTCTC+TGG + Chr1:88898832-88898851 None:intergenic 50.0%
!! TGGAGGAATTCTTGGGTTGG+TGG - Chr1:88901503-88901522 MsG0180005231.01.T01:CDS 50.0%
AACTGTGAGACTGCACGGGA+GGG + Chr1:88899202-88899221 None:intergenic 55.0%
AGGGCTGGAGAAGATGAAGC+TGG + Chr1:88898805-88898824 None:intergenic 55.0%
ATCCCGAATGGAATGGGTGC+TGG + Chr1:88898619-88898638 None:intergenic 55.0%
ATGAGCCACTCCGTGCTATC+TGG - Chr1:88898727-88898746 MsG0180005231.01.T01:CDS 55.0%
ATGTAACCGACCCCACTTGG+TGG - Chr1:88899744-88899763 MsG0180005231.01.T01:intron 55.0%
CAGAGGCGATCCAGATAGCA+CGG + Chr1:88898740-88898759 None:intergenic 55.0%
GAGTGGCTCATACGGTACGT+CGG + Chr1:88898718-88898737 None:intergenic 55.0%
GCTCATACGGTACGTCGGAT+TGG + Chr1:88898713-88898732 None:intergenic 55.0%
GGGACATTGTGTTGGGCATG+TGG - Chr1:88900311-88900330 MsG0180005231.01.T01:intron 55.0%
GGGACCCCAACAAACTACCT+AGG - Chr1:88901101-88901120 MsG0180005231.01.T01:CDS 55.0%
TGTAACCGACCCCACTTGGT+GGG - Chr1:88899745-88899764 MsG0180005231.01.T01:intron 55.0%
! GGGTCTAGTGGATGGATCAG+AGG + Chr1:88898757-88898776 None:intergenic 55.0%
CAACTGTGAGACTGCACGGG+AGG + Chr1:88899203-88899222 None:intergenic 60.0%
GCGATCCAGATAGCACGGAG+TGG + Chr1:88898735-88898754 None:intergenic 60.0%
TTCGGCGGAGAGAGTGGTGT+CGG + Chr1:88898661-88898680 None:intergenic 60.0%
!! AGTGGTGTCGGTGGAGGCTT+CGG + Chr1:88898649-88898668 None:intergenic 60.0%
TCGGAGTTCGGCGGAGAGAG+TGG + Chr1:88898667-88898686 None:intergenic 65.0%
!! GGAGAGAGTGGTGTCGGTGG+AGG + Chr1:88898655-88898674 None:intergenic 65.0%
! GGCGGAGAGAGTGGTGTCGG+TGG + Chr1:88898658-88898677 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 88898493 88901820 88898493 ID=MsG0180005231.01;Name=MsG0180005231.01
Chr1 mRNA 88898493 88901820 88898493 ID=MsG0180005231.01.T01;Parent=MsG0180005231.01;Name=MsG0180005231.01.T01;_AED=0.46;_eAED=0.46;_QI=47|0.5|0.6|1|0.25|0.2|5|167|273
Chr1 exon 88898493 88898818 88898493 ID=MsG0180005231.01.T01:exon:37411;Parent=MsG0180005231.01.T01
Chr1 exon 88899334 88899419 88899334 ID=MsG0180005231.01.T01:exon:37410;Parent=MsG0180005231.01.T01
Chr1 exon 88900807 88900985 88900807 ID=MsG0180005231.01.T01:exon:37409;Parent=MsG0180005231.01.T01
Chr1 exon 88901086 88901183 88901086 ID=MsG0180005231.01.T01:exon:37408;Parent=MsG0180005231.01.T01
Chr1 exon 88901474 88901820 88901474 ID=MsG0180005231.01.T01:exon:37407;Parent=MsG0180005231.01.T01
Chr1 five_prime_UTR 88901774 88901820 88901774 ID=MsG0180005231.01.T01:five_prime_utr;Parent=MsG0180005231.01.T01
Chr1 CDS 88901474 88901773 88901474 ID=MsG0180005231.01.T01:cds;Parent=MsG0180005231.01.T01
Chr1 CDS 88901086 88901183 88901086 ID=MsG0180005231.01.T01:cds;Parent=MsG0180005231.01.T01
Chr1 CDS 88900807 88900985 88900807 ID=MsG0180005231.01.T01:cds;Parent=MsG0180005231.01.T01
Chr1 CDS 88899334 88899419 88899334 ID=MsG0180005231.01.T01:cds;Parent=MsG0180005231.01.T01
Chr1 CDS 88898660 88898818 88898660 ID=MsG0180005231.01.T01:cds;Parent=MsG0180005231.01.T01
Chr1 three_prime_UTR 88898493 88898659 88898493 ID=MsG0180005231.01.T01:three_prime_utr;Parent=MsG0180005231.01.T01
Gene Sequence

>MsG0180005231.01.T01

ATGAATGATGTGTGCCACCATCAGAAACAAAAAATGGCTACTGAAAACATGTCGAATCGAGTCGGGTTAGTAATATCCAGCACCCATTCCATTCGGGATTTTCTCTCCGAAGCCTCCACCGACACCACTCTCTCCGCCGAACTCCGACAAAAATGCTCCGATCTGCTCATCCAATCCGACGTACCGTATGAGCCACTCCGTGCTATCTGGATCGCCTCTGATCCATCCACTAGACCCGAATTGACCCAACTCTTATCGGGTACCAGCTTCATCTTCTCCAGCCCTAAACCCAGAGAAAAGCTATTCTCTAAAGCTTATGGAAATAAGCTGAAAATAGCTTATAGACATGTAATAAACTGTTTTCATAAGCCCTCCCGTGCAGTCTCACAGTTGCTTATCAATGCAAGTAACTTTCATGTAGACTCTTTTGGTGTGAAGAGTGAAGAATTGAAGGCAAGGTTAAAAAAATTGGAAGATATAGCAGAGAGGAAAGCATATCAAGAGCTTGTGAAGGATATTGCACCAAAGGAAGATGTTGCAGAACCATTCTCTTCTTACAAGGATCAATTAGGATTTGGTTTGCATGTTTTGGTTACAATGTTTACTGGCTATCTTGTTGGATATGCAGCATTTAGAGCTCTGTTTGATCACAGTCCTGCCATGAATGCAGCTGGAGGAATTCTTGGGTTGGTGGGTGCCATGCTTGTTGAGACTTTCCTTTTCATTATTAGAAATTCCGATCTAGATAGCAACAAAAAAAGGAAATCAAATCAAAAACCGAGATCATCATTTTCCACATCCAGTCTCAAGAAAAACCTGTAG
Protein sequence

>MsG0180005231.01.T01

MNDVCHHQKQKMATENMSNRVGLVISSTHSIRDFLSEASTDTTLSAELRQKCSDLLIQSDVPYEPLRAIWIASDPSTRPELTQLLSGTSFIFSSPKPREKLFSKAYGNKLKIAYRHVINCFHKPSRAVSQLLINASNFHVDSFGVKSEELKARLKKLEDIAERKAYQELVKDIAPKEDVAEPFSSYKDQLGFGLHVLVTMFTGYLVGYAAFRALFDHSPAMNAAGGILGLVGAMLVETFLFIIRNSDLDSNKKRKSNQKPRSSFSTSSLKKNL*