AlfalfaGEDB | MsG0180005231.01

M. sativa cultivar ZhongmuNo.1 / MsG0180005231.01

KEGG: -
GO: GO:0070072
PFAM: PF11712

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005231.01.T01	XP_003591956.1	77.481	262	13	1	12	273	1	216	2.33E-137	396

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005231.01.T01	G8A2K5	77.481	262	13	1	12	273	1	216	1.11e-137	396

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180005231.01	MsG0280007691.01	0.805209	1.491083e-49	7.842259e-47
MsG0180005231.01	MsG0280008295.01	0.804291	2.317973e-49	1.190656e-46
MsG0180005231.01	MsG0480019289.01	0.800913	1.153220e-48	5.435026e-46
MsG0180005231.01	MsG0480023581.01	0.801366	9.314932e-49	4.440487e-46
MsG0180005231.01	MsG0580024374.01	0.802915	4.472184e-49	2.217514e-46
MsG0180005231.01	MsG0580026596.01	0.820257	7.548440e-53	5.942765e-50
MsG0180005231.01	MsG0580029629.01	0.806258	8.977856e-50	4.850781e-47
MsG0180005231.01	MsG0880045106.01	0.807167	5.769807e-50	3.191089e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005231.01.T01	MTR_1g095620	77.481	262	13	1	12	273	1	216	2.83e-141	396

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005231.01.T01	AT5G52980	51.362	257	79	1	12	268	1	211	1.03e-78	238
MsG0180005231.01.T01	AT5G52980	50.000	210	59	1	12	221	1	164	3.87e-61	192

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Find 64 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACATGTCGAATCGAGTC+GGG	0.481485	1:-88901708	MsG0180005231.01.T01:CDS
AAACCTGTAGACATGATCAA+AGG	0.628970	1:-88898647	MsG0180005231.01.T01:exon
AACTGTGAGACTGCACGGGA+GGG	0.593767	1:+88901092	None:intergenic
AACTTTCATGTAGACTCTTT+TGG	0.327754	1:-88900953	MsG0180005231.01.T01:CDS
AAGCAACTGTGAGACTGCAC+GGG	0.623346	1:+88901088	None:intergenic
AAGCTATTCTCTAAAGCTTA+TGG	0.295378	1:-88901164	MsG0180005231.01.T01:CDS
AAGGAAAGTCTCAACAAGCA+TGG	0.624200	1:+88898762	None:intergenic
AATGCAGCTGGAGGAATTCT+TGG	0.319711	1:-88898796	MsG0180005231.01.T01:CDS
AATTGGAAGATATAGCAGAG+AGG	0.675662	1:-88900895	MsG0180005231.01.T01:CDS
AGAACCATTCTCTTCTTACA+AGG	0.500737	1:-88900822	MsG0180005231.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATACTTCAGAGATATGC+AGG	+	Chr1:88900845-88900864	None:intergenic	30.0%
AACGACAATAACATAAAGAG+GGG	+	Chr1:88901396-88901415	None:intergenic	30.0%
ACTTCTATAAACAAAACCTC+TGG	+	Chr1:88899911-88899930	None:intergenic	30.0%
AGCAAAATTGTAGTTGTGAT+TGG	+	Chr1:88900508-88900527	None:intergenic	30.0%
ATGTTAGTAGTATATGTTGG+TGG	-	Chr1:88900387-88900406	MsG0180005231.01.T01:intron	30.0%
ATTACAGTGTATTGCATACT+TGG	+	Chr1:88899868-88899887	None:intergenic	30.0%
CGGCATATAGTTTATTAAGT+TGG	+	Chr1:88901152-88901171	None:intergenic	30.0%
CTACATGGTTTATGATAGTT+TGG	+	Chr1:88901361-88901380	None:intergenic	30.0%
CTGTTTCCTATTAAGTTATG+AGG	-	Chr1:88899095-88899114	MsG0180005231.01.T01:intron	30.0%
CTTGATTGAACTTAGTAACA+TGG	-	Chr1:88900231-88900250	MsG0180005231.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	88898493	88901820	88898493	ID=MsG0180005231.01;Name=MsG0180005231.01
Chr1	mRNA	88898493	88901820	88898493	ID=MsG0180005231.01.T01;Parent=MsG0180005231.01;Name=MsG0180005231.01.T01;_AED=0.46;_eAED=0.46;_QI=47\|0.5\|0.6\|1\|0.25\|0.2\|5\|167\|273
Chr1	exon	88898493	88898818	88898493	ID=MsG0180005231.01.T01:exon:37411;Parent=MsG0180005231.01.T01
Chr1	exon	88899334	88899419	88899334	ID=MsG0180005231.01.T01:exon:37410;Parent=MsG0180005231.01.T01
Chr1	exon	88900807	88900985	88900807	ID=MsG0180005231.01.T01:exon:37409;Parent=MsG0180005231.01.T01
Chr1	exon	88901086	88901183	88901086	ID=MsG0180005231.01.T01:exon:37408;Parent=MsG0180005231.01.T01
Chr1	exon	88901474	88901820	88901474	ID=MsG0180005231.01.T01:exon:37407;Parent=MsG0180005231.01.T01
Chr1	five_prime_UTR	88901774	88901820	88901774	ID=MsG0180005231.01.T01:five_prime_utr;Parent=MsG0180005231.01.T01
Chr1	CDS	88901474	88901773	88901474	ID=MsG0180005231.01.T01:cds;Parent=MsG0180005231.01.T01
Chr1	CDS	88901086	88901183	88901086	ID=MsG0180005231.01.T01:cds;Parent=MsG0180005231.01.T01
Chr1	CDS	88900807	88900985	88900807	ID=MsG0180005231.01.T01:cds;Parent=MsG0180005231.01.T01
Chr1	CDS	88899334	88899419	88899334	ID=MsG0180005231.01.T01:cds;Parent=MsG0180005231.01.T01
Chr1	CDS	88898660	88898818	88898660	ID=MsG0180005231.01.T01:cds;Parent=MsG0180005231.01.T01
Chr1	three_prime_UTR	88898493	88898659	88898493	ID=MsG0180005231.01.T01:three_prime_utr;Parent=MsG0180005231.01.T01

Gene Sequence

>MsG0180005231.01.T01

ATGAATGATGTGTGCCACCATCAGAAACAAAAAATGGCTACTGAAAACATGTCGAATCGAGTCGGGTTAGTAATATCCAGCACCCATTCCATTCGGGATTTTCTCTCCGAAGCCTCCACCGACACCACTCTCTCCGCCGAACTCCGACAAAAATGCTCCGATCTGCTCATCCAATCCGACGTACCGTATGAGCCACTCCGTGCTATCTGGATCGCCTCTGATCCATCCACTAGACCCGAATTGACCCAACTCTTATCGGGTACCAGCTTCATCTTCTCCAGCCCTAAACCCAGAGAAAAGCTATTCTCTAAAGCTTATGGAAATAAGCTGAAAATAGCTTATAGACATGTAATAAACTGTTTTCATAAGCCCTCCCGTGCAGTCTCACAGTTGCTTATCAATGCAAGTAACTTTCATGTAGACTCTTTTGGTGTGAAGAGTGAAGAATTGAAGGCAAGGTTAAAAAAATTGGAAGATATAGCAGAGAGGAAAGCATATCAAGAGCTTGTGAAGGATATTGCACCAAAGGAAGATGTTGCAGAACCATTCTCTTCTTACAAGGATCAATTAGGATTTGGTTTGCATGTTTTGGTTACAATGTTTACTGGCTATCTTGTTGGATATGCAGCATTTAGAGCTCTGTTTGATCACAGTCCTGCCATGAATGCAGCTGGAGGAATTCTTGGGTTGGTGGGTGCCATGCTTGTTGAGACTTTCCTTTTCATTATTAGAAATTCCGATCTAGATAGCAACAAAAAAAGGAAATCAAATCAAAAACCGAGATCATCATTTTCCACATCCAGTCTCAAGAAAAACCTGTAG

Protein sequence

>MsG0180005231.01.T01

MNDVCHHQKQKMATENMSNRVGLVISSTHSIRDFLSEASTDTTLSAELRQKCSDLLIQSDVPYEPLRAIWIASDPSTRPELTQLLSGTSFIFSSPKPREKLFSKAYGNKLKIAYRHVINCFHKPSRAVSQLLINASNFHVDSFGVKSEELKARLKKLEDIAERKAYQELVKDIAPKEDVAEPFSSYKDQLGFGLHVLVTMFTGYLVGYAAFRALFDHSPAMNAAGGILGLVGAMLVETFLFIIRNSDLDSNKKRKSNQKPRSSFSTSSLKKNL*