AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005256.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005256.01.T01 MTR_1g495705 99.355 155 1 0 1 155 1 155 1.66e-111 324
MsG0180005256.01.T01 MTR_1g095650 99.355 155 1 0 1 155 1 155 1.66e-111 324
MsG0180005256.01.T01 MTR_1g095703 99.355 155 1 0 1 155 1 155 2.08e-111 324
MsG0180005256.01.T01 MTR_4g014810 98.710 155 2 0 1 155 1 155 3.68e-111 323
MsG0180005256.01.T01 MTR_6g021800 98.710 155 2 0 1 155 1 155 3.72e-111 323
MsG0180005256.01.T01 MTR_6g021805 98.710 155 2 0 1 155 1 155 3.72e-111 323
MsG0180005256.01.T01 MTR_1g013680 94.304 158 9 0 1 158 1 158 4.48e-105 308
MsG0180005256.01.T01 MTR_1g085410 85.806 155 22 0 1 155 1 155 2.94e-95 283
MsG0180005256.01.T01 MTR_1g101880 82.317 164 29 0 1 164 1 164 1.13e-92 270
MsG0180005256.01.T01 MTR_1g024175 80.000 155 31 0 1 155 1 155 2.32e-88 266
MsG0180005256.01.T01 MTR_1g101870 85.806 155 20 1 1 155 1 153 4.07e-88 274
MsG0180005256.01.T01 MTR_1g101870 85.806 155 20 1 1 155 1 153 8.63e-88 274
MsG0180005256.01.T01 MTR_8g008740 80.128 156 30 1 1 155 1 156 4.60e-87 263
MsG0180005256.01.T01 MTR_8g014590 80.892 157 28 1 1 155 1 157 6.86e-82 249
MsG0180005256.01.T01 MTR_1g024165 80.851 141 27 0 5 145 9 149 9.49e-82 238
MsG0180005256.01.T01 MTR_5g037350 62.632 190 34 1 3 155 13 202 9.16e-77 239
MsG0180005256.01.T01 MTR_2g080210 73.418 158 39 2 1 155 1 158 1.46e-76 235
MsG0180005256.01.T01 MTR_8g013910 71.795 156 41 2 1 155 1 154 5.28e-72 223
MsG0180005256.01.T01 MTR_2g082730 74.834 151 23 1 5 155 5 140 5.44e-72 225
MsG0180005256.01.T01 MTR_1g089520 71.711 152 26 3 4 155 5 139 8.55e-68 215
MsG0180005256.01.T01 MTR_2g037420 65.839 161 37 2 1 161 1 143 1.11e-65 207
MsG0180005256.01.T01 MTR_8g014950 76.471 102 24 0 3 104 4 105 4.62e-51 159
MsG0180005256.01.T01 MTR_2g082665 60.265 151 19 1 5 155 5 114 2.92e-50 168
MsG0180005256.01.T01 MTR_8g087950 52.000 150 71 1 7 155 275 424 4.09e-49 168
MsG0180005256.01.T01 MTR_8g087950 52.000 150 71 1 7 155 348 497 6.69e-49 168
MsG0180005256.01.T01 MTR_6g080420 46.358 151 81 0 5 155 89 239 4.30e-45 155
MsG0180005256.01.T01 MTR_8g106700 45.395 152 83 0 4 155 43 194 2.39e-44 152
MsG0180005256.01.T01 MTR_8g106700 45.395 152 83 0 4 155 49 200 3.18e-44 152
MsG0180005256.01.T01 MTR_8g106700 45.395 152 83 0 4 155 70 221 3.68e-44 152
MsG0180005256.01.T01 MTR_1g095650 98.148 54 1 0 102 155 1 54 1.97e-31 115
MsG0180005256.01.T01 MTR_7g094460 35.220 159 81 2 3 161 59 195 5.26e-25 100
MsG0180005256.01.T01 MTR_2g021300 35.849 159 80 2 3 161 46 182 6.26e-25 99.8
MsG0180005256.01.T01 MTR_3g076660 34.868 152 77 2 3 154 88 217 2.42e-24 98.6
MsG0180005256.01.T01 MTR_1g495735 71.698 53 15 0 21 73 2 54 3.53e-19 82.4
MsG0180005256.01.T01 MTR_7g098190 63.077 65 14 3 56 118 10 66 5.25e-15 69.3
MsG0180005256.01.T01 MTR_4g054970 75.000 44 11 0 1 44 1 44 1.77e-14 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005256.01.T01 AT1G07930 98.710 155 2 0 1 155 1 155 6.07e-111 320
MsG0180005256.01.T01 AT5G60390 98.710 155 2 0 1 155 1 155 1.40e-110 322
MsG0180005256.01.T01 AT5G60390 98.710 155 2 0 1 155 1 155 1.40e-110 322
MsG0180005256.01.T01 AT5G60390 98.710 155 2 0 1 155 1 155 1.40e-110 322
MsG0180005256.01.T01 AT1G07920 98.710 155 2 0 1 155 1 155 1.40e-110 322
MsG0180005256.01.T01 AT1G07940 98.710 155 2 0 1 155 1 155 1.40e-110 322
MsG0180005256.01.T01 AT1G07940 98.710 155 2 0 1 155 1 155 1.40e-110 322
MsG0180005256.01.T01 AT1G07940 98.710 155 2 0 1 155 1 155 1.40e-110 322
MsG0180005256.01.T01 AT1G07940 98.710 155 2 0 1 155 1 155 1.40e-110 322
MsG0180005256.01.T01 AT1G07930 98.710 155 2 0 1 155 1 155 1.40e-110 322
MsG0180005256.01.T01 AT5G10630 47.333 150 78 1 7 155 239 388 7.38e-46 159
MsG0180005256.01.T01 AT5G10630 47.333 150 78 1 7 155 240 389 7.44e-46 159
MsG0180005256.01.T01 AT5G10630 47.333 150 78 1 7 155 240 389 7.44e-46 159
MsG0180005256.01.T01 AT5G10630 47.333 150 78 1 7 155 310 459 1.19e-45 159
MsG0180005256.01.T01 AT5G10630 47.333 150 78 1 7 155 315 464 1.22e-45 159
MsG0180005256.01.T01 AT1G18070 45.033 151 83 0 5 155 99 249 6.86e-43 149
MsG0180005256.01.T01 AT1G18070 45.033 151 83 0 5 155 99 249 6.86e-43 149
MsG0180005256.01.T01 AT1G18070 45.033 151 83 0 5 155 99 249 9.75e-43 149
MsG0180005256.01.T01 AT4G20360 36.842 152 74 2 3 154 75 204 4.59e-26 103
MsG0180005256.01.T01 AT4G20360 36.842 152 74 2 3 154 75 204 4.59e-26 103
MsG0180005256.01.T01 AT4G02930 35.849 159 80 3 3 161 63 199 9.39e-25 99.4

Find 46 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCTCCTTGCTTTCACTCT+TGG 0.289982 1:-89236214 MsG0180005256.01.T01:CDS
GTCATCGGCCATGTTGACTC+TGG 0.440627 1:-89236589 MsG0180005256.01.T01:CDS
AGGACTAATGGGTAAGGAAA+AGG 0.440646 1:-89236629 None:intergenic
AGCGTTCATTCAAGTATGCC+TGG 0.444655 1:-89236471 MsG0180005256.01.T01:CDS
TAATCAAATATACAACTTCA+AGG 0.464604 1:+89236141 None:intergenic
TGCACTGTCATTGATGCCCC+TGG 0.477178 1:-89236364 MsG0180005256.01.T01:CDS
TTAATGAAATCCCTGTGTCC+AGG 0.479234 1:+89236346 None:intergenic
TCACCATTGACATTGCTCTC+TGG 0.481623 1:-89236411 MsG0180005256.01.T01:CDS
ACAGTCAGCTTGGGATGTTC+CGG 0.483896 1:+89236312 None:intergenic
CTCATCATTGACTCCACCAC+TGG 0.490200 1:-89236283 MsG0180005256.01.T01:CDS
AATCAAATATACAACTTCAA+GGG 0.494892 1:+89236142 None:intergenic
TGAGAACGGCACAGTCAGCT+TGG 0.498317 1:+89236302 None:intergenic
CTGACCATCCTTAGAAATAC+CGG 0.501461 1:+89236249 None:intergenic
GAGAACGGCACAGTCAGCTT+GGG 0.505334 1:+89236303 None:intergenic
AATGACAGTGCAGTAGTACT+TGG 0.509426 1:+89236375 None:intergenic
ATCATTGACTCCACCACTGG+TGG 0.514298 1:-89236280 MsG0180005256.01.T01:CDS
TCTGGGAAATCGACCACCAC+TGG 0.515893 1:-89236571 MsG0180005256.01.T01:CDS
CACATCAACATTGTGGTCAT+CGG 0.522862 1:-89236604 MsG0180005256.01.T01:CDS
TAATGAAATCCCTGTGTCCA+GGG 0.528272 1:+89236347 None:intergenic
CATTGATGCCCCTGGACACA+GGG 0.535845 1:-89236356 MsG0180005256.01.T01:CDS
TCATTGATGCCCCTGGACAC+AGG 0.539611 1:-89236357 MsG0180005256.01.T01:CDS
CTTCCAGAGAGCAATGTCAA+TGG 0.541189 1:+89236408 None:intergenic
CACTTGATCTACAAGCTTGG+AGG 0.545798 1:-89236547 MsG0180005256.01.T01:CDS
GGTCACTTGATCTACAAGCT+TGG 0.555547 1:-89236550 MsG0180005256.01.T01:CDS
ATCAAATATACAACTTCAAG+GGG 0.556106 1:+89236143 None:intergenic
AAGCAAGGAGAGCATGTTCA+CGG 0.559179 1:+89236224 None:intergenic
CTCAAGGCTGAACGTGAAAG+AGG 0.559327 1:-89236436 MsG0180005256.01.T01:CDS
GAAGCCGGTATTTCTAAGGA+TGG 0.564413 1:-89236253 MsG0180005256.01.T01:CDS
TCATCGGCCATGTTGACTCT+GGG 0.571507 1:-89236588 MsG0180005256.01.T01:CDS
TTCATTAAGAACATGATCAC+CGG 0.572337 1:-89236331 MsG0180005256.01.T01:CDS
AGCAAGGAGAGCATGTTCAC+GGG 0.572470 1:+89236225 None:intergenic
CTTGAGCTTGTCAAGGACCC+AGG 0.577041 1:+89236453 None:intergenic
GGCTTCAAAACCACCAGTGG+TGG 0.579933 1:+89236270 None:intergenic
TTGACAAGCGTGTCATTGAG+AGG 0.584549 1:-89236522 MsG0180005256.01.T01:CDS
CTGGGTCCTTGACAAGCTCA+AGG 0.585742 1:-89236452 MsG0180005256.01.T01:CDS
GCGTTCATTCAAGTATGCCT+GGG 0.605879 1:-89236470 MsG0180005256.01.T01:CDS
TCGATTTCCCAGAGTCAACA+TGG 0.615058 1:+89236581 None:intergenic
TCACACCAAGAGTGAAAGCA+AGG 0.621193 1:+89236209 None:intergenic
GTTCAGCCTTGAGCTTGTCA+AGG 0.628494 1:+89236446 None:intergenic
GGTGGAGTCAATGATGAGAA+CGG 0.642252 1:+89236288 None:intergenic
AATGAAATCCCTGTGTCCAG+GGG 0.656838 1:+89236348 None:intergenic
ACCGGCTTCAAAACCACCAG+TGG 0.659375 1:+89236267 None:intergenic
AATGATCTGCTGTTGCAACA+AGG 0.659392 1:-89236182 MsG0180005256.01.T01:CDS
GTAGATCAAGTGACCAGTGG+TGG 0.666416 1:+89236558 None:intergenic
AAAGGTTCACATCAACATTG+TGG 0.675059 1:-89236611 MsG0180005256.01.T01:CDS
CTTGTAGATCAAGTGACCAG+TGG 0.682705 1:+89236555 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTCATTAAGAACATGATCAC+CGG - Chr1:89236435-89236454 MsG0180005256.01.T01:CDS 30.0%
AAAGGTTCACATCAACATTG+TGG - Chr1:89236155-89236174 MsG0180005256.01.T01:CDS 35.0%
!!! TTTTGAAGCCGGTATTTCTA+AGG - Chr1:89236509-89236528 MsG0180005256.01.T01:CDS 35.0%
AATGACAGTGCAGTAGTACT+TGG + Chr1:89236394-89236413 None:intergenic 40.0%
AATGATCTGCTGTTGCAACA+AGG - Chr1:89236584-89236603 MsG0180005256.01.T01:CDS 40.0%
CACATCAACATTGTGGTCAT+CGG - Chr1:89236162-89236181 MsG0180005256.01.T01:CDS 40.0%
CTGACCATCCTTAGAAATAC+CGG + Chr1:89236520-89236539 None:intergenic 40.0%
TAATGAAATCCCTGTGTCCA+GGG + Chr1:89236422-89236441 None:intergenic 40.0%
TTAATGAAATCCCTGTGTCC+AGG + Chr1:89236423-89236442 None:intergenic 40.0%
AATGAAATCCCTGTGTCCAG+GGG + Chr1:89236421-89236440 None:intergenic 45.0%
AGCGTTCATTCAAGTATGCC+TGG - Chr1:89236295-89236314 MsG0180005256.01.T01:CDS 45.0%
CACTTGATCTACAAGCTTGG+AGG - Chr1:89236219-89236238 MsG0180005256.01.T01:CDS 45.0%
CTTCCAGAGAGCAATGTCAA+TGG + Chr1:89236361-89236380 None:intergenic 45.0%
CTTGTAGATCAAGTGACCAG+TGG + Chr1:89236214-89236233 None:intergenic 45.0%
GCGTTCATTCAAGTATGCCT+GGG - Chr1:89236296-89236315 MsG0180005256.01.T01:CDS 45.0%
GGTCACTTGATCTACAAGCT+TGG - Chr1:89236216-89236235 MsG0180005256.01.T01:CDS 45.0%
GGTGGAGTCAATGATGAGAA+CGG + Chr1:89236481-89236500 None:intergenic 45.0%
TCACACCAAGAGTGAAAGCA+AGG + Chr1:89236560-89236579 None:intergenic 45.0%
TCACCATTGACATTGCTCTC+TGG - Chr1:89236355-89236374 MsG0180005256.01.T01:CDS 45.0%
TCGATTTCCCAGAGTCAACA+TGG + Chr1:89236188-89236207 None:intergenic 45.0%
! AAGCAAGGAGAGCATGTTCA+CGG + Chr1:89236545-89236564 None:intergenic 45.0%
! GAAGCCGGTATTTCTAAGGA+TGG - Chr1:89236513-89236532 MsG0180005256.01.T01:CDS 45.0%
! TTGACAAGCGTGTCATTGAG+AGG - Chr1:89236244-89236263 MsG0180005256.01.T01:CDS 45.0%
ACAGTCAGCTTGGGATGTTC+CGG + Chr1:89236457-89236476 None:intergenic 50.0%
CTCAAGGCTGAACGTGAAAG+AGG - Chr1:89236330-89236349 MsG0180005256.01.T01:CDS 50.0%
GCTCTCCTTGCTTTCACTCT+TGG - Chr1:89236552-89236571 MsG0180005256.01.T01:CDS 50.0%
GTAGATCAAGTGACCAGTGG+TGG + Chr1:89236211-89236230 None:intergenic 50.0%
GTTCAGCCTTGAGCTTGTCA+AGG + Chr1:89236323-89236342 None:intergenic 50.0%
! AGCAAGGAGAGCATGTTCAC+GGG + Chr1:89236544-89236563 None:intergenic 50.0%
!! ATCATTGACTCCACCACTGG+TGG - Chr1:89236486-89236505 MsG0180005256.01.T01:CDS 50.0%
!! CTCATCATTGACTCCACCAC+TGG - Chr1:89236483-89236502 MsG0180005256.01.T01:CDS 50.0%
!! TCATCGGCCATGTTGACTCT+GGG - Chr1:89236178-89236197 MsG0180005256.01.T01:CDS 50.0%
!!! ACCACTGGTGGTTTTGAAGC+CGG - Chr1:89236498-89236517 MsG0180005256.01.T01:CDS 50.0%
CATTGATGCCCCTGGACACA+GGG - Chr1:89236410-89236429 MsG0180005256.01.T01:CDS 55.0%
CTGGGTCCTTGACAAGCTCA+AGG - Chr1:89236314-89236333 MsG0180005256.01.T01:CDS 55.0%
CTTGAGCTTGTCAAGGACCC+AGG + Chr1:89236316-89236335 None:intergenic 55.0%
GAGAACGGCACAGTCAGCTT+GGG + Chr1:89236466-89236485 None:intergenic 55.0%
GGCTTCAAAACCACCAGTGG+TGG + Chr1:89236499-89236518 None:intergenic 55.0%
TCATTGATGCCCCTGGACAC+AGG - Chr1:89236409-89236428 MsG0180005256.01.T01:CDS 55.0%
TCTGGGAAATCGACCACCAC+TGG - Chr1:89236195-89236214 MsG0180005256.01.T01:CDS 55.0%
TGAGAACGGCACAGTCAGCT+TGG + Chr1:89236467-89236486 None:intergenic 55.0%
TGCACTGTCATTGATGCCCC+TGG - Chr1:89236402-89236421 MsG0180005256.01.T01:CDS 55.0%
! ACCGGCTTCAAAACCACCAG+TGG + Chr1:89236502-89236521 None:intergenic 55.0%
!! GTCATCGGCCATGTTGACTC+TGG - Chr1:89236177-89236196 MsG0180005256.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 89236144 89236644 89236144 ID=MsG0180005256.01;Name=MsG0180005256.01
Chr1 mRNA 89236144 89236644 89236144 ID=MsG0180005256.01.T01;Parent=MsG0180005256.01;Name=MsG0180005256.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|166
Chr1 exon 89236144 89236644 89236144 ID=MsG0180005256.01.T01:exon:14606;Parent=MsG0180005256.01.T01
Chr1 CDS 89236144 89236644 89236144 ID=MsG0180005256.01.T01:cds;Parent=MsG0180005256.01.T01
Gene Sequence

>MsG0180005256.01.T01

ATGGGTAAGGAAAAGGTTCACATCAACATTGTGGTCATCGGCCATGTTGACTCTGGGAAATCGACCACCACTGGTCACTTGATCTACAAGCTTGGAGGTATTGACAAGCGTGTCATTGAGAGGTTTGAGAAAGAAGCTGCTGAGATGAACAAGCGTTCATTCAAGTATGCCTGGGTCCTTGACAAGCTCAAGGCTGAACGTGAAAGAGGTATCACCATTGACATTGCTCTCTGGAAGTTTGAGACAACCAAGTACTACTGCACTGTCATTGATGCCCCTGGACACAGGGATTTCATTAAGAACATGATCACCGGAACATCCCAAGCTGACTGTGCCGTTCTCATCATTGACTCCACCACTGGTGGTTTTGAAGCCGGTATTTCTAAGGATGGTCAGACCCGTGAACATGCTCTCCTTGCTTTCACTCTTGGTGTGAAGCAAATGATCTGCTGTTGCAACAAGGTATTTAATATTTTCAGCCCCTTGAAGTTGTATATTTGA

Protein sequence

>MsG0180005256.01.T01

MGKEKVHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKVFNIFSPLKLYI*