Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005273.01.T01 | XP_013469501.1 | 90.323 | 62 | 6 | 0 | 10 | 71 | 144 | 205 | 9.26E-33 | 122 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005273.01.T01 | A0A072VNF1 | 90.323 | 62 | 6 | 0 | 10 | 71 | 144 | 205 | 4.42e-33 | 122 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180005273.01 | MsG0680033745.01 | 0.804389 | 2.211873e-49 | 1.138982e-46 |
| MsG0180005273.01 | MsG0780040160.01 | 0.818299 | 2.107072e-52 | 1.571177e-49 |
| MsG0180005273.01 | MsG0780040292.01 | 0.823633 | 1.246856e-53 | 1.079577e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005273.01.T01 | MTR_1g096170 | 90.323 | 62 | 6 | 0 | 10 | 71 | 144 | 205 | 1.12e-36 | 122 |
| MsG0180005273.01.T01 | MTR_1g096170 | 90.323 | 62 | 6 | 0 | 10 | 71 | 139 | 200 | 1.36e-36 | 121 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACTATAGTCGGAGAGCTTT+TGG | 0.185399 | 1:-89457200 | MsG0180005273.01.T01:CDS |
| GACTATAGTTGGAGAGCTTT+TGG | 0.218783 | 1:-89457285 | MsG0180005273.01.T01:CDS |
| TGCATCAGGGCGACGTGGCC+TGG | 0.360741 | 1:+89457151 | None:intergenic |
| TGGATAACGCTAGCCGTTCC+AGG | 0.375993 | 1:-89457169 | MsG0180005273.01.T01:CDS |
| GAGATTGGGCTAACTGCATC+AGG | 0.387744 | 1:+89457137 | None:intergenic |
| TCACTTTGGTGGCAACCATT+TGG | 0.393886 | 1:-89457222 | MsG0180005273.01.T01:CDS |
| TCACTTTGGTGGCAACCATT+TGG | 0.393886 | 1:-89457307 | MsG0180005273.01.T01:CDS |
| CTCTCCAACTATAGTCCAAA+TGG | 0.427689 | 1:+89457292 | None:intergenic |
| TAGTAAGGTGGGTAAGAGAT+TGG | 0.436729 | 1:+89457122 | None:intergenic |
| GCAACCATTTGGACTATAGT+TGG | 0.444374 | 1:-89457296 | MsG0180005273.01.T01:CDS |
| CATTAGATTGGTCGGTTGCA+CGG | 0.455879 | 1:+89457255 | None:intergenic |
| CTCTCCGACTATAGTCCAAA+TGG | 0.467158 | 1:+89457207 | None:intergenic |
| ATTGAAAATATTAGTAAGGT+GGG | 0.489596 | 1:+89457111 | None:intergenic |
| ATCTATTGAAAATATTAGTA+AGG | 0.490677 | 1:+89457107 | None:intergenic |
| GAGATCCATACGCATTAGAT+TGG | 0.492253 | 1:+89457243 | None:intergenic |
| AGTAAGGTGGGTAAGAGATT+GGG | 0.492624 | 1:+89457123 | None:intergenic |
| TCCATACGCATTAGATTGGT+CGG | 0.528133 | 1:+89457247 | None:intergenic |
| ACCGACCAATCTAATGCGTA+TGG | 0.537772 | 1:-89457248 | MsG0180005273.01.T01:CDS |
| TATTGAAAATATTAGTAAGG+TGG | 0.542457 | 1:+89457110 | None:intergenic |
| AATGCGTATGGATCTCACTT+TGG | 0.547698 | 1:-89457236 | MsG0180005273.01.T01:CDS |
| AATGCGTATGGATCTCACTT+TGG | 0.547698 | 1:-89457321 | None:intergenic |
| CAGGGCGACGTGGCCTGGAA+CGG | 0.550905 | 1:+89457156 | None:intergenic |
| GCGTATGGATCTCACTTTGG+TGG | 0.555553 | 1:-89457233 | MsG0180005273.01.T01:CDS |
| GCGTATGGATCTCACTTTGG+TGG | 0.555553 | 1:-89457318 | None:intergenic |
| GCAACCATTTGGACTATAGT+CGG | 0.610307 | 1:-89457211 | MsG0180005273.01.T01:CDS |
| CTAACTGCATCAGGGCGACG+TGG | 0.635502 | 1:+89457146 | None:intergenic |
| AGATTGGGCTAACTGCATCA+GGG | 0.658192 | 1:+89457138 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACTATAGTTGGAGAGCTTTT+GGG | - | Chr1:89457151-89457170 | MsG0180005273.01.T01:CDS | 35.0% |
| AATGCGTATGGATCTCACTT+TGG | - | Chr1:89457199-89457218 | MsG0180005273.01.T01:CDS | 40.0% | |
| AGTAAGGTGGGTAAGAGATT+GGG | + | Chr1:89457315-89457334 | None:intergenic | 40.0% | |
| CTCTCCAACTATAGTCCAAA+TGG | + | Chr1:89457146-89457165 | None:intergenic | 40.0% | |
| GAGATCCATACGCATTAGAT+TGG | + | Chr1:89457195-89457214 | None:intergenic | 40.0% | |
| GCAACCATTTGGACTATAGT+CGG | - | Chr1:89457139-89457158 | MsG0180005273.01.T01:CDS | 40.0% | |
| GCAACCATTTGGACTATAGT+TGG | - | Chr1:89457139-89457158 | MsG0180005273.01.T01:CDS | 40.0% | |
| TAGTAAGGTGGGTAAGAGAT+TGG | + | Chr1:89457316-89457335 | None:intergenic | 40.0% | |
| TCCATACGCATTAGATTGGT+CGG | + | Chr1:89457191-89457210 | None:intergenic | 40.0% | |
| ! | ACTATAGTCGGAGAGCTTTT+GGG | - | Chr1:89457236-89457255 | MsG0180005273.01.T01:CDS | 40.0% |
| ! | CTATAGTTGGAGAGCTTTTG+GGG | - | Chr1:89457152-89457171 | MsG0180005273.01.T01:CDS | 40.0% |
| ! | GACTATAGTTGGAGAGCTTT+TGG | - | Chr1:89457150-89457169 | MsG0180005273.01.T01:CDS | 40.0% |
| ACCGACCAATCTAATGCGTA+TGG | - | Chr1:89457187-89457206 | MsG0180005273.01.T01:CDS | 45.0% | |
| AGATTGGGCTAACTGCATCA+GGG | + | Chr1:89457300-89457319 | None:intergenic | 45.0% | |
| CTCTCCGACTATAGTCCAAA+TGG | + | Chr1:89457231-89457250 | None:intergenic | 45.0% | |
| ! | CATTAGATTGGTCGGTTGCA+CGG | + | Chr1:89457183-89457202 | None:intergenic | 45.0% |
| ! | CTATAGTCGGAGAGCTTTTG+GGG | - | Chr1:89457237-89457256 | MsG0180005273.01.T01:CDS | 45.0% |
| ! | GACTATAGTCGGAGAGCTTT+TGG | - | Chr1:89457235-89457254 | MsG0180005273.01.T01:CDS | 45.0% |
| ! | TCACTTTGGTGGCAACCATT+TGG | - | Chr1:89457128-89457147 | MsG0180005273.01.T01:CDS | 45.0% |
| GAGATTGGGCTAACTGCATC+AGG | + | Chr1:89457301-89457320 | None:intergenic | 50.0% | |
| GCGTATGGATCTCACTTTGG+TGG | - | Chr1:89457202-89457221 | MsG0180005273.01.T01:CDS | 50.0% | |
| !! | TAGTTGGAGAGCTTTTGGGG+AGG | - | Chr1:89457155-89457174 | MsG0180005273.01.T01:CDS | 50.0% |
| TGGATAACGCTAGCCGTTCC+AGG | - | Chr1:89457266-89457285 | MsG0180005273.01.T01:CDS | 55.0% | |
| !! | GAGAGCTTTTGGGGAGGAAC+TGG | - | Chr1:89457161-89457180 | MsG0180005273.01.T01:CDS | 55.0% |
| !! | TAGTCGGAGAGCTTTTGGGG+AGG | - | Chr1:89457240-89457259 | MsG0180005273.01.T01:CDS | 55.0% |
| CTAACTGCATCAGGGCGACG+TGG | + | Chr1:89457292-89457311 | None:intergenic | 60.0% | |
| CAGGGCGACGTGGCCTGGAA+CGG | + | Chr1:89457282-89457301 | None:intergenic | 70.0% | |
| TGCATCAGGGCGACGTGGCC+TGG | + | Chr1:89457287-89457306 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 89457121 | 89457336 | 89457121 | ID=MsG0180005273.01;Name=MsG0180005273.01 |
| Chr1 | mRNA | 89457121 | 89457336 | 89457121 | ID=MsG0180005273.01.T01;Parent=MsG0180005273.01;Name=MsG0180005273.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|71 |
| Chr1 | exon | 89457121 | 89457336 | 89457121 | ID=MsG0180005273.01.T01:exon:47762;Parent=MsG0180005273.01.T01 |
| Chr1 | CDS | 89457121 | 89457336 | 89457121 | ID=MsG0180005273.01.T01:cds;Parent=MsG0180005273.01.T01 |
| Gene Sequence |
| Protein sequence |
