Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680033745.01.T01 | XP_039682702.1 | 99 | 100 | 1 | 0 | 1 | 100 | 795 | 894 | 4.92E-57 | 199 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680033745.01.T01 | A0A396HGZ2 | 99.000 | 100 | 1 | 0 | 1 | 100 | 795 | 894 | 2.35e-57 | 199 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004651.01 | MsG0680033745.01 | 0.801069 | 1.071516e-48 | 5.069893e-46 |
| MsG0180005273.01 | MsG0680033745.01 | 0.804389 | 2.211873e-49 | 1.138982e-46 |
| MsG0180006069.01 | MsG0680033745.01 | 0.800222 | 1.594467e-48 | 7.383680e-46 |
| MsG0280006407.01 | MsG0680033745.01 | 0.814706 | 1.343827e-51 | 9.082328e-49 |
| MsG0380012054.01 | MsG0680033745.01 | 0.821118 | 4.785242e-53 | 3.858645e-50 |
| MsG0380013754.01 | MsG0680033745.01 | 0.810097 | 1.365651e-50 | 8.158581e-48 |
| MsG0680031816.01 | MsG0680033745.01 | 0.855666 | 5.412311e-62 | 1.259111e-58 |
| MsG0680033745.01 | MsG0780036876.01 | 0.820121 | 8.106115e-53 | 6.358706e-50 |
| MsG0680033745.01 | MsG0780039844.01 | 0.804408 | 2.191913e-49 | 1.129225e-46 |
| MsG0680033745.01 | MsG0780040205.01 | 0.842005 | 3.367229e-58 | 5.036035e-55 |
| MsG0680033745.01 | MsG0780040239.01 | 0.820817 | 5.612968e-53 | 4.488285e-50 |
| MsG0680033745.01 | MsG0880046375.01 | 0.817802 | 2.729881e-52 | 2.008031e-49 |
| MsG0480021495.01 | MsG0680033745.01 | 0.831122 | 1.996362e-55 | 2.146105e-52 |
| MsG0480021953.01 | MsG0680033745.01 | 0.825692 | 4.079928e-54 | 3.745910e-51 |
| MsG0480022971.01 | MsG0680033745.01 | 0.809389 | 1.938779e-50 | 1.136854e-47 |
| MsG0480023765.01 | MsG0680033745.01 | 0.807447 | 5.033255e-50 | 2.804093e-47 |
| MsG0580024265.01 | MsG0680033745.01 | 0.821332 | 4.271100e-53 | 3.464624e-50 |
| MsG0380016346.01 | MsG0680033745.01 | 0.828199 | 1.026763e-54 | 1.013504e-51 |
| MsG0380016605.01 | MsG0680033745.01 | 0.807733 | 4.376761e-50 | 2.456573e-47 |
| MsG0380017135.01 | MsG0680033745.01 | -0.800175 | 1.630616e-48 | 7.541741e-46 |
| MsG0480018126.01 | MsG0680033745.01 | 0.820163 | 7.928840e-53 | 6.226442e-50 |
| MsG0280006544.01 | MsG0680033745.01 | 0.873946 | 9.911493e-68 | 4.380200e-64 |
| MsG0280007088.01 | MsG0680033745.01 | 0.816259 | 6.063885e-52 | 4.275963e-49 |
| MsG0280009789.01 | MsG0680033745.01 | 0.813107 | 3.026390e-51 | 1.958449e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATATGGCAAAGAATAATT+TGG | 0.196446 | 6:+72522792 | None:intergenic |
| GCAAAGAATAATTTGGGTTT+TGG | 0.226566 | 6:+72522799 | MsG0680033745.01.T01:CDS |
| CTCCATTTGTGGTTGGAGTT+TGG | 0.238832 | 6:-72522825 | None:intergenic |
| GCCAATGGTCAAGGAAATAA+TGG | 0.328082 | 6:+72522856 | MsG0680033745.01.T01:CDS |
| AATATGGCAAAGAATAATTT+GGG | 0.333666 | 6:+72522793 | None:intergenic |
| CGCTTTCTTTAACAGTGATC+TGG | 0.346150 | 6:+72523044 | MsG0680033745.01.T01:CDS |
| GGCAGAGCCTCCTCCATTTG+TGG | 0.385110 | 6:-72522836 | None:intergenic |
| TTGAGTCAGTCATGGCTTCC+AGG | 0.414836 | 6:-72523085 | None:intergenic |
| AAACTGTTACTCCTTGAAAC+AGG | 0.415397 | 6:-72522880 | None:intergenic |
| AAAGCGCTGTCTGTGAAGTC+AGG | 0.416020 | 6:-72523027 | None:intergenic |
| GGCCAAACTCCAACCACAAA+TGG | 0.432260 | 6:+72522823 | MsG0680033745.01.T01:CDS |
| TGTGAAGTCAGGGGCAAAAT+CGG | 0.442003 | 6:-72523016 | None:intergenic |
| GACTCTTCTGCAGCAGCTGG+TGG | 0.454343 | 6:+72522922 | MsG0680033745.01.T01:CDS |
| TCTTCTGCAGCAGCTGGTGG+AGG | 0.457790 | 6:+72522925 | MsG0680033745.01.T01:CDS |
| CAGTGATCTGGATGATAACA+TGG | 0.459525 | 6:+72523056 | MsG0680033745.01.T01:CDS |
| ATCGGAAGCAACGTCTTGTA+AGG | 0.467151 | 6:-72522998 | None:intergenic |
| GAAATAATGGACCTGTTTCA+AGG | 0.474603 | 6:+72522869 | MsG0680033745.01.T01:CDS |
| TCCATTATTTCCTTGACCAT+TGG | 0.493782 | 6:-72522857 | None:intergenic |
| TCTGCAGCAGCTGGTGGAGG+CGG | 0.500960 | 6:+72522928 | MsG0680033745.01.T01:CDS |
| GCTGGTGGAGGCGGCAACAA+TGG | 0.501813 | 6:+72522937 | MsG0680033745.01.T01:CDS |
| GAGCCTCCTCCATTTGTGGT+TGG | 0.509004 | 6:-72522832 | None:intergenic |
| AGTGACTCTTCTGCAGCAGC+TGG | 0.518460 | 6:+72522919 | MsG0680033745.01.T01:CDS |
| GCGACATATCAGATGTCCTC+TGG | 0.526090 | 6:-72522966 | None:intergenic |
| GGAGGCTCTGCCAATGGTCA+AGG | 0.550765 | 6:+72522847 | MsG0680033745.01.T01:CDS |
| AAGCGCTGTCTGTGAAGTCA+GGG | 0.568237 | 6:-72523026 | None:intergenic |
| AATGGAGGAGGCTCTGCCAA+TGG | 0.596871 | 6:+72522841 | MsG0680033745.01.T01:CDS |
| CAAACTCCAACCACAAATGG+AGG | 0.635025 | 6:+72522826 | MsG0680033745.01.T01:CDS |
| ACTCCAACCACAAATGGAGG+AGG | 0.636114 | 6:+72522829 | MsG0680033745.01.T01:CDS |
| GCAACAATGGATTCAACCAG+AGG | 0.677444 | 6:+72522950 | MsG0680033745.01.T01:CDS |
| AGTGATCTGGATGATAACAT+GGG | 0.697820 | 6:+72523057 | MsG0680033745.01.T01:CDS |
| AGCGCTGTCTGTGAAGTCAG+GGG | 0.701664 | 6:-72523025 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAGAATAATTTGGGTTTTG+GGG | + | Chr6:72522801-72522820 | MsG0680033745.01.T01:CDS | 25.0% |
| !!! | CAAAGAATAATTTGGGTTTT+GGG | + | Chr6:72522800-72522819 | MsG0680033745.01.T01:CDS | 25.0% |
| !! | AAGAATAATTTGGGTTTTGG+GGG | + | Chr6:72522802-72522821 | MsG0680033745.01.T01:CDS | 30.0% |
| !! | GCAAAGAATAATTTGGGTTT+TGG | + | Chr6:72522799-72522818 | MsG0680033745.01.T01:CDS | 30.0% |
| AAACTGTTACTCCTTGAAAC+AGG | - | Chr6:72522883-72522902 | None:intergenic | 35.0% | |
| AGTGATCTGGATGATAACAT+GGG | + | Chr6:72523057-72523076 | MsG0680033745.01.T01:CDS | 35.0% | |
| TCCATTATTTCCTTGACCAT+TGG | - | Chr6:72522860-72522879 | None:intergenic | 35.0% | |
| ! | ATGATAACATGGGTTTTACC+TGG | + | Chr6:72523067-72523086 | MsG0680033745.01.T01:CDS | 35.0% |
| !! | GAAATAATGGACCTGTTTCA+AGG | + | Chr6:72522869-72522888 | MsG0680033745.01.T01:CDS | 35.0% |
| CAGTGATCTGGATGATAACA+TGG | + | Chr6:72523056-72523075 | MsG0680033745.01.T01:CDS | 40.0% | |
| CGCTTTCTTTAACAGTGATC+TGG | + | Chr6:72523044-72523063 | MsG0680033745.01.T01:CDS | 40.0% | |
| GCCAATGGTCAAGGAAATAA+TGG | + | Chr6:72522856-72522875 | MsG0680033745.01.T01:CDS | 40.0% | |
| ATCGGAAGCAACGTCTTGTA+AGG | - | Chr6:72523001-72523020 | None:intergenic | 45.0% | |
| CAAACTCCAACCACAAATGG+AGG | + | Chr6:72522826-72522845 | MsG0680033745.01.T01:CDS | 45.0% | |
| CTCCATTTGTGGTTGGAGTT+TGG | - | Chr6:72522828-72522847 | None:intergenic | 45.0% | |
| TGTGAAGTCAGGGGCAAAAT+CGG | - | Chr6:72523019-72523038 | None:intergenic | 45.0% | |
| ! | GCAACAATGGATTCAACCAG+AGG | + | Chr6:72522950-72522969 | MsG0680033745.01.T01:CDS | 45.0% |
| ACTCCAACCACAAATGGAGG+AGG | + | Chr6:72522829-72522848 | MsG0680033745.01.T01:CDS | 50.0% | |
| GCGACATATCAGATGTCCTC+TGG | - | Chr6:72522969-72522988 | None:intergenic | 50.0% | |
| GGCCAAACTCCAACCACAAA+TGG | + | Chr6:72522823-72522842 | MsG0680033745.01.T01:CDS | 50.0% | |
| ! | AAGCGCTGTCTGTGAAGTCA+GGG | - | Chr6:72523029-72523048 | None:intergenic | 50.0% |
| !! | AAAGCGCTGTCTGTGAAGTC+AGG | - | Chr6:72523030-72523049 | None:intergenic | 50.0% |
| AATGGAGGAGGCTCTGCCAA+TGG | + | Chr6:72522841-72522860 | MsG0680033745.01.T01:CDS | 55.0% | |
| AGCGCTGTCTGTGAAGTCAG+GGG | - | Chr6:72523028-72523047 | None:intergenic | 55.0% | |
| AGTGACTCTTCTGCAGCAGC+TGG | + | Chr6:72522919-72522938 | MsG0680033745.01.T01:CDS | 55.0% | |
| GAGCCTCCTCCATTTGTGGT+TGG | - | Chr6:72522835-72522854 | None:intergenic | 55.0% | |
| GACTCTTCTGCAGCAGCTGG+TGG | + | Chr6:72522922-72522941 | MsG0680033745.01.T01:CDS | 60.0% | |
| GGAGGCTCTGCCAATGGTCA+AGG | + | Chr6:72522847-72522866 | MsG0680033745.01.T01:CDS | 60.0% | |
| GGCAGAGCCTCCTCCATTTG+TGG | - | Chr6:72522839-72522858 | None:intergenic | 60.0% | |
| TCTTCTGCAGCAGCTGGTGG+AGG | + | Chr6:72522925-72522944 | MsG0680033745.01.T01:CDS | 60.0% | |
| GCTGGTGGAGGCGGCAACAA+TGG | + | Chr6:72522937-72522956 | MsG0680033745.01.T01:CDS | 65.0% | |
| TCTGCAGCAGCTGGTGGAGG+CGG | + | Chr6:72522928-72522947 | MsG0680033745.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 72522796 | 72523098 | 72522796 | ID=MsG0680033745.01;Name=MsG0680033745.01 |
| Chr6 | mRNA | 72522796 | 72523098 | 72522796 | ID=MsG0680033745.01.T01;Parent=MsG0680033745.01;Name=MsG0680033745.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|100 |
| Chr6 | exon | 72522796 | 72523098 | 72522796 | ID=MsG0680033745.01.T01:exon:4102;Parent=MsG0680033745.01.T01 |
| Chr6 | CDS | 72522796 | 72523098 | 72522796 | ID=MsG0680033745.01.T01:cds;Parent=MsG0680033745.01.T01 |
| Gene Sequence |
| Protein sequence |
