AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005781.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005781.01.T01 MTR_1g106955 99.061 213 2 0 1 213 1 213 7.58e-156 430
MsG0180005781.01.T01 MTR_8g076890 42.675 157 88 2 25 180 105 260 1.86e-38 134
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005781.01.T01 AT2G18990 77.885 208 45 1 6 213 5 211 1.41e-119 338
MsG0180005781.01.T01 AT3G25580 77.404 208 45 2 6 213 5 210 1.38e-118 336
MsG0180005781.01.T01 AT3G25580 77.073 205 45 2 9 213 3 205 1.30e-116 330
MsG0180005781.01.T01 AT5G66410 36.585 205 123 4 7 209 28 227 1.09e-38 133
MsG0180005781.01.T01 AT3G50960 42.000 150 85 2 23 171 40 188 4.08e-37 129
MsG0180005781.01.T01 AT3G50960 42.000 150 85 2 23 171 40 188 4.08e-37 129

Find 61 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGACAAGCATTTAAGTATTT+TGG 0.171906 1:+96104830 MsG0180005781.01.T01:CDS
GTTGCAGCCTCCTTTCTCTT+AGG 0.207376 1:-96104167 None:intergenic
TATCAATTGCCAGTCTTGTT+TGG 0.266888 1:+96105467 MsG0180005781.01.T01:three_prime_UTR
GCAAAGGACAAACACATTAA+AGG 0.268507 1:-96105563 None:intergenic
TACTGCATAAAAGTAATTCT+TGG 0.286458 1:-96105599 None:intergenic
TGACTTTAGCACCGAGGTTT+TGG 0.292143 1:+96105142 MsG0180005781.01.T01:CDS
GTCATTTCTTCCGCGAAAAT+TGG 0.299383 1:+96104310 MsG0180005781.01.T01:CDS
CGAAATTGTGCGAACCTTGC+AGG 0.328390 1:-96104334 None:intergenic
TTGCAGCCTCCTTTCTCTTA+GGG 0.338559 1:-96104166 None:intergenic
ATCTGAGCTCTCGTCTTTCC+AGG 0.348336 1:-96105332 None:intergenic
GCTCTGCCGAACACTTCTTT+TGG 0.354451 1:-96105243 None:intergenic
CTTATAGTGATTAGAATCAT+TGG 0.355260 1:-96105507 None:intergenic
TCAATACACCGTGCAAGGTC+CGG 0.394040 1:+96105212 MsG0180005781.01.T01:CDS
GCTGCAACAAATGAAGAAAA+TGG 0.402598 1:+96104182 MsG0180005781.01.T01:CDS
CTTGATTCTCGATCACCTCT+TGG 0.414878 1:-96104059 None:intergenic
CAATACACCGTGCAAGGTCC+GGG 0.431500 1:+96105213 MsG0180005781.01.T01:CDS
CGAGGGAGAGAGATCGATCT+AGG 0.451921 1:+96104010 MsG0180005781.01.T01:five_prime_UTR
AACATTTGCTTCATGACTAC+AGG 0.477196 1:-96105630 None:intergenic
TTTGCGCCCCGGACCTTGCA+CGG 0.490890 1:-96105220 None:intergenic
GTCTTTCTCTGACGGAATCT+CGG 0.495284 1:-96104250 None:intergenic
CGCAAACCAAAAGAAGTGTT+CGG 0.506657 1:+96105237 MsG0180005781.01.T01:CDS
CGGTGTAATCTCCGTGTCCG+AGG 0.513907 1:-96104230 None:intergenic
CGTCATCGTCGAGACGTTCA+AGG 0.517999 1:-96104134 None:intergenic
AGAATCATTGGTACAATCAT+CGG 0.521116 1:-96105495 None:intergenic
ATCTTGAAGCCCTAAGAGAA+AGG 0.527404 1:+96104157 MsG0180005781.01.T01:CDS
CGAAGCAGCATATTGAGACT+CGG 0.534134 1:+96104853 MsG0180005781.01.T01:CDS
TTCTTTATGAGAGCAATAGT+AGG 0.546329 1:-96104939 None:intergenic
TTCGTCGATCTTATCCTCGA+CGG 0.549709 1:-96104100 None:intergenic
TGGTATTAGCACATTTGCAA+AGG 0.556981 1:-96105579 None:intergenic
CCGCGAAAATTGGCCCTGCA+AGG 0.557879 1:+96104320 MsG0180005781.01.T01:CDS
TACCAAAGTGGATGATTATG+TGG 0.558275 1:+96104962 MsG0180005781.01.T01:CDS
GAAATTGTGCGAACCTTGCA+GGG 0.562815 1:-96104333 None:intergenic
GTCATCGTCGAGACGTTCAA+GGG 0.565214 1:-96104133 None:intergenic
TCGGATAATCCAAACAAGAC+TGG 0.569214 1:-96105476 None:intergenic
GAGAAGAAGTCTTTCTCTGA+CGG 0.577836 1:-96104258 None:intergenic
AATCATCAATACACCGTGCA+AGG 0.579819 1:+96105207 MsG0180005781.01.T01:CDS
CGTTGGATCTCCCTCGGACA+CGG 0.582223 1:+96104219 MsG0180005781.01.T01:CDS
TACCACATAATCATCCACTT+TGG 0.582640 1:-96104964 None:intergenic
CGTAGCCGTTGGATCTCCCT+CGG 0.582964 1:+96104213 MsG0180005781.01.T01:CDS
TGGCGGAGAAGCGTAGCCGT+TGG 0.601854 1:+96104202 MsG0180005781.01.T01:CDS
CAATCTTTCTTCCAAAACCT+CGG 0.602383 1:-96105153 None:intergenic
AATTTAGAAAGACACACAAA+CGG 0.604177 1:-96105381 None:intergenic
ACGATCACTTGCTTTAACGA+CGG 0.607682 1:-96104280 None:intergenic
GGTGTAATCTCCGTGTCCGA+GGG 0.608291 1:-96104229 None:intergenic
TACAAGTTACTCAGAATCTG+AGG 0.610014 1:-96105279 None:intergenic
GAACTCAAAGTGCAGAGCGA+GGG 0.616104 1:+96103993 None:intergenic
GAGCTCTCGTCTTTCCAGGA+GGG 0.622232 1:-96105328 None:intergenic
TGAGCTCTCGTCTTTCCAGG+AGG 0.628602 1:-96105329 None:intergenic
GACGGTGGCTCAAGCCGTCG+AGG 0.631972 1:+96104086 MsG0180005781.01.T01:CDS
CGTGTCCGAGGGAGATCCAA+CGG 0.633581 1:-96104218 None:intergenic
TGAACTCAAAGTGCAGAGCG+AGG 0.639978 1:+96103992 None:intergenic
AATACACCGTGCAAGGTCCG+GGG 0.655774 1:+96105214 MsG0180005781.01.T01:CDS
GATCGAGAATCAAGTACTGA+CGG 0.657827 1:+96104068 MsG0180005781.01.T01:CDS
TCTCATAAAGAATACCAAAG+TGG 0.658073 1:+96104950 MsG0180005781.01.T01:CDS
TTGAAGCCCTAAGAGAAAGG+AGG 0.672465 1:+96104160 MsG0180005781.01.T01:CDS
TACTGATGACTTTAGCACCG+AGG 0.705317 1:+96105136 MsG0180005781.01.T01:CDS
GCAACAAATGAAGAAAATGG+CGG 0.709368 1:+96104185 MsG0180005781.01.T01:CDS
AAGTGTTCGGCAGAGCACAA+CGG 0.717985 1:+96105250 MsG0180005781.01.T01:CDS
GGACAAAGGAAAGATCCAAG+AGG 0.724397 1:+96104044 MsG0180005781.01.T01:CDS
CGAGAATCAAGTACTGACGG+TGG 0.724611 1:+96104071 MsG0180005781.01.T01:CDS
AAACTTACAAGACACACGAG+TGG 0.733578 1:-96105416 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAACTTTATAAAAATATTAT+TGG + Chr1:96104585-96104604 MsG0180005781.01.T01:intron 10.0%
!!! AAGAATTTTAATTTAAACGT+AGG + Chr1:96104563-96104582 MsG0180005781.01.T01:intron 15.0%
!! ACATGTGATTACATAATTAT+CGG + Chr1:96104450-96104469 MsG0180005781.01.T01:intron 20.0%
!! TAGAATTATCTGTTTATCAT+TGG + Chr1:96104680-96104699 MsG0180005781.01.T01:intron 20.0%
!!! CTTTCTAAATTTAGTTTTGT+TGG + Chr1:96105393-96105412 MsG0180005781.01.T01:three_prime_UTR 20.0%
!!! TGTCTAAATTTTGTTTTTGT+TGG + Chr1:96105358-96105377 MsG0180005781.01.T01:three_prime_UTR 20.0%
! AATTATCTGTTTATCATTGG+AGG + Chr1:96104683-96104702 MsG0180005781.01.T01:intron 25.0%
! AATTATGTAATCACATGTGT+AGG - Chr1:96104448-96104467 None:intergenic 25.0%
! AATTTAGAAAGACACACAAA+CGG - Chr1:96105384-96105403 None:intergenic 25.0%
! ATATAAAAACTGTAAGTCTG+CGG - Chr1:96104754-96104773 None:intergenic 25.0%
! ATTATCTGTTTATCATTGGA+GGG + Chr1:96104684-96104703 MsG0180005781.01.T01:intron 25.0%
! CTTATAGTGATTAGAATCAT+TGG - Chr1:96105510-96105529 None:intergenic 25.0%
! TACTAACATCAAGATGATAA+TGG + Chr1:96105078-96105097 MsG0180005781.01.T01:intron 25.0%
! TACTGCATAAAAGTAATTCT+TGG - Chr1:96105602-96105621 None:intergenic 25.0%
! TATTGACATGAATGCTATAT+TGG + Chr1:96105025-96105044 MsG0180005781.01.T01:intron 25.0%
!!! ATGATAATGGTATGTGTTTT+AGG + Chr1:96105091-96105110 MsG0180005781.01.T01:intron 25.0%
!!! TAATACTAAGAGTTTTGAGA+TGG + Chr1:96105530-96105549 MsG0180005781.01.T01:three_prime_UTR 25.0%
AGAATCATTGGTACAATCAT+CGG - Chr1:96105498-96105517 None:intergenic 30.0%
CGGAATTCAAATTCAAATTG+TGG - Chr1:96104734-96104753 None:intergenic 30.0%
GATTATGTGGTATGTCTTAA+AGG + Chr1:96104975-96104994 MsG0180005781.01.T01:intron 30.0%
TCTCATAAAGAATACCAAAG+TGG + Chr1:96104950-96104969 MsG0180005781.01.T01:CDS 30.0%
TGATTACATAATTATCGGTG+TGG + Chr1:96104455-96104474 MsG0180005781.01.T01:intron 30.0%
TTCTTTATGAGAGCAATAGT+AGG - Chr1:96104942-96104961 None:intergenic 30.0%
! GGACAAGCATTTAAGTATTT+TGG + Chr1:96104830-96104849 MsG0180005781.01.T01:CDS 30.0%
! TAAAGCTCAAGTCATCTTTT+TGG + Chr1:96105178-96105197 MsG0180005781.01.T01:CDS 30.0%
! TATTTTCTTTCGCTGAAATG+AGG + Chr1:96104771-96104790 MsG0180005781.01.T01:intron 30.0%
!! ATAATGGTATGTGTTTTAGG+TGG + Chr1:96105094-96105113 MsG0180005781.01.T01:intron 30.0%
!! TAATGGTATGTGTTTTAGGT+GGG + Chr1:96105095-96105114 MsG0180005781.01.T01:intron 30.0%
AACATTTGCTTCATGACTAC+AGG - Chr1:96105633-96105652 None:intergenic 35.0%
ACATGAATGCTATATTGGAC+AGG + Chr1:96105030-96105049 MsG0180005781.01.T01:intron 35.0%
AGGAGTGAAAAAATGGACAA+AGG + Chr1:96104030-96104049 MsG0180005781.01.T01:exon 35.0%
ATATTATTGGCAGTGCATAC+TGG + Chr1:96104598-96104617 MsG0180005781.01.T01:intron 35.0%
CAATCTTTCTTCCAAAACCT+CGG - Chr1:96105156-96105175 None:intergenic 35.0%
CATGAATGCTATATTGGACA+GGG + Chr1:96105031-96105050 MsG0180005781.01.T01:intron 35.0%
GCAAAGGACAAACACATTAA+AGG - Chr1:96105566-96105585 None:intergenic 35.0%
GCAACAAATGAAGAAAATGG+CGG + Chr1:96104185-96104204 MsG0180005781.01.T01:CDS 35.0%
GCTGCAACAAATGAAGAAAA+TGG + Chr1:96104182-96104201 MsG0180005781.01.T01:CDS 35.0%
TACAAGTTACTCAGAATCTG+AGG - Chr1:96105282-96105301 None:intergenic 35.0%
TACCAAAGTGGATGATTATG+TGG + Chr1:96104962-96104981 MsG0180005781.01.T01:CDS 35.0%
TACCACATAATCATCCACTT+TGG - Chr1:96104967-96104986 None:intergenic 35.0%
TCGATCTAGGAGTGAAAAAA+TGG + Chr1:96104023-96104042 MsG0180005781.01.T01:exon 35.0%
TGGTATTAGCACATTTGCAA+AGG - Chr1:96105582-96105601 None:intergenic 35.0%
TTGAGCTTCTCAGCTAAAAA+TGG - Chr1:96104906-96104925 None:intergenic 35.0%
! TGCATTCAGTTTGATTAGCT+TGG + Chr1:96104535-96104554 MsG0180005781.01.T01:intron 35.0%
!! TATCAATTGCCAGTCTTGTT+TGG + Chr1:96105467-96105486 MsG0180005781.01.T01:three_prime_UTR 35.0%
AAACTTACAAGACACACGAG+TGG - Chr1:96105419-96105438 None:intergenic 40.0%
AATCATCAATACACCGTGCA+AGG + Chr1:96105207-96105226 MsG0180005781.01.T01:CDS 40.0%
ACGATCACTTGCTTTAACGA+CGG - Chr1:96104283-96104302 None:intergenic 40.0%
ATCTTGAAGCCCTAAGAGAA+AGG + Chr1:96104157-96104176 MsG0180005781.01.T01:CDS 40.0%
GAGAAGAAGTCTTTCTCTGA+CGG - Chr1:96104261-96104280 None:intergenic 40.0%
GTAATCACATGTGTAGGTGT+CGG - Chr1:96104442-96104461 None:intergenic 40.0%
GTCATTTCTTCCGCGAAAAT+TGG + Chr1:96104310-96104329 MsG0180005781.01.T01:CDS 40.0%
TCGGATAATCCAAACAAGAC+TGG - Chr1:96105479-96105498 None:intergenic 40.0%
! AGCTCAAGTCATCTTTTTGG+AGG + Chr1:96105181-96105200 MsG0180005781.01.T01:CDS 40.0%
! GCTCAAGTCATCTTTTTGGA+GGG + Chr1:96105182-96105201 MsG0180005781.01.T01:CDS 40.0%
!! CGCAAACCAAAAGAAGTGTT+CGG + Chr1:96105237-96105256 MsG0180005781.01.T01:CDS 40.0%
!! GATCGAGAATCAAGTACTGA+CGG + Chr1:96104068-96104087 MsG0180005781.01.T01:CDS 40.0%
!! TAGGTGGGTTTTGATGAACT+TGG + Chr1:96105110-96105129 MsG0180005781.01.T01:intron 40.0%
!! TTTTATTTACGTTGCCCTCC+TGG + Chr1:96105314-96105333 MsG0180005781.01.T01:three_prime_UTR 40.0%
!!! CGAGGTTTTGGAAGAAAGAT+TGG + Chr1:96105154-96105173 MsG0180005781.01.T01:CDS 40.0%
CACAGTTCATTCACAGGTTG+TGG + Chr1:96104809-96104828 MsG0180005781.01.T01:intron 45.0%
CGAAGCAGCATATTGAGACT+CGG + Chr1:96104853-96104872 MsG0180005781.01.T01:CDS 45.0%
CTTGATTCTCGATCACCTCT+TGG - Chr1:96104062-96104081 None:intergenic 45.0%
GAAATTGTGCGAACCTTGCA+GGG - Chr1:96104336-96104355 None:intergenic 45.0%
GGACAAAGGAAAGATCCAAG+AGG + Chr1:96104044-96104063 MsG0180005781.01.T01:CDS 45.0%
GTCTTTCTCTGACGGAATCT+CGG - Chr1:96104253-96104272 None:intergenic 45.0%
TACTGATGACTTTAGCACCG+AGG + Chr1:96105136-96105155 MsG0180005781.01.T01:CDS 45.0%
TGTGCTCACAGTTCATTCAC+AGG + Chr1:96104803-96104822 MsG0180005781.01.T01:intron 45.0%
TTCGTCGATCTTATCCTCGA+CGG - Chr1:96104103-96104122 None:intergenic 45.0%
TTGAAGCCCTAAGAGAAAGG+AGG + Chr1:96104160-96104179 MsG0180005781.01.T01:CDS 45.0%
TTGCAGCCTCCTTTCTCTTA+GGG - Chr1:96104169-96104188 None:intergenic 45.0%
!! GTGGGTTTTGATGAACTTGG+TGG + Chr1:96105113-96105132 MsG0180005781.01.T01:CDS 45.0%
!! TGACTTTAGCACCGAGGTTT+TGG + Chr1:96105142-96105161 MsG0180005781.01.T01:CDS 45.0%
AAGTGTTCGGCAGAGCACAA+CGG + Chr1:96105250-96105269 MsG0180005781.01.T01:CDS 50.0%
ATCTGAGCTCTCGTCTTTCC+AGG - Chr1:96105335-96105354 None:intergenic 50.0%
CGAAATTGTGCGAACCTTGC+AGG - Chr1:96104337-96104356 None:intergenic 50.0%
CGGTGTGGATGTGTAGTGTT+AGG + Chr1:96104470-96104489 MsG0180005781.01.T01:intron 50.0%
GTCATCGTCGAGACGTTCAA+GGG - Chr1:96104136-96104155 None:intergenic 50.0%
GTTGCAGCCTCCTTTCTCTT+AGG - Chr1:96104170-96104189 None:intergenic 50.0%
TCAATACACCGTGCAAGGTC+CGG + Chr1:96105212-96105231 MsG0180005781.01.T01:CDS 50.0%
! GCTCTGCCGAACACTTCTTT+TGG - Chr1:96105246-96105265 None:intergenic 50.0%
!! CGAGAATCAAGTACTGACGG+TGG + Chr1:96104071-96104090 MsG0180005781.01.T01:CDS 50.0%
!!! ACTTCTTTTGGTTTGCGCCC+CGG - Chr1:96105234-96105253 None:intergenic 50.0%
AATACACCGTGCAAGGTCCG+GGG + Chr1:96105214-96105233 MsG0180005781.01.T01:CDS 55.0%
CAATACACCGTGCAAGGTCC+GGG + Chr1:96105213-96105232 MsG0180005781.01.T01:CDS 55.0%
CGAGGGAGAGAGATCGATCT+AGG + Chr1:96104010-96104029 MsG0180005781.01.T01:five_prime_UTR 55.0%
CGTCATCGTCGAGACGTTCA+AGG - Chr1:96104137-96104156 None:intergenic 55.0%
GAGCTCTCGTCTTTCCAGGA+GGG - Chr1:96105331-96105350 None:intergenic 55.0%
GGTGTAATCTCCGTGTCCGA+GGG - Chr1:96104232-96104251 None:intergenic 55.0%
TGAGCTCTCGTCTTTCCAGG+AGG - Chr1:96105332-96105351 None:intergenic 55.0%
! CCTTGCAGGGCCAATTTTCG+CGG - Chr1:96104323-96104342 None:intergenic 55.0%
CCGCGAAAATTGGCCCTGCA+AGG + Chr1:96104320-96104339 MsG0180005781.01.T01:CDS 60.0%
CGGTGTAATCTCCGTGTCCG+AGG - Chr1:96104233-96104252 None:intergenic 60.0%
CGTAGCCGTTGGATCTCCCT+CGG + Chr1:96104213-96104232 MsG0180005781.01.T01:CDS 60.0%
CGTGTCCGAGGGAGATCCAA+CGG - Chr1:96104221-96104240 None:intergenic 60.0%
CGTTGGATCTCCCTCGGACA+CGG + Chr1:96104219-96104238 MsG0180005781.01.T01:CDS 60.0%
TTTGCGCCCCGGACCTTGCA+CGG - Chr1:96105223-96105242 None:intergenic 65.0%
! TGGCGGAGAAGCGTAGCCGT+TGG + Chr1:96104202-96104221 MsG0180005781.01.T01:CDS 65.0%
GACGGTGGCTCAAGCCGTCG+AGG + Chr1:96104086-96104105 MsG0180005781.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 96104001 96105686 96104001 ID=MsG0180005781.01;Name=MsG0180005781.01
Chr1 mRNA 96104001 96105686 96104001 ID=MsG0180005781.01.T01;Parent=MsG0180005781.01;Name=MsG0180005781.01.T01;_AED=0.49;_eAED=0.49;_QI=41|1|1|1|1|1|3|391|213
Chr1 exon 96104001 96104341 96104001 ID=MsG0180005781.01.T01:exon:10540;Parent=MsG0180005781.01.T01
Chr1 exon 96104825 96104983 96104825 ID=MsG0180005781.01.T01:exon:10541;Parent=MsG0180005781.01.T01
Chr1 exon 96105113 96105686 96105113 ID=MsG0180005781.01.T01:exon:10542;Parent=MsG0180005781.01.T01
Chr1 five_prime_UTR 96104001 96104041 96104001 ID=MsG0180005781.01.T01:five_prime_utr;Parent=MsG0180005781.01.T01
Chr1 CDS 96104042 96104341 96104042 ID=MsG0180005781.01.T01:cds;Parent=MsG0180005781.01.T01
Chr1 CDS 96104825 96104983 96104825 ID=MsG0180005781.01.T01:cds;Parent=MsG0180005781.01.T01
Chr1 CDS 96105113 96105295 96105113 ID=MsG0180005781.01.T01:cds;Parent=MsG0180005781.01.T01
Chr1 three_prime_UTR 96105296 96105686 96105296 ID=MsG0180005781.01.T01:three_prime_utr;Parent=MsG0180005781.01.T01
Gene Sequence

>MsG0180005781.01.T01

ATGGACAAAGGAAAGATCCAAGAGGTGATCGAGAATCAAGTACTGACGGTGGCTCAAGCCGTCGAGGATAAGATCGACGAAGAGATTGCTGCCCTTGAACGTCTCGACGATGACGATCTTGAAGCCCTAAGAGAAAGGAGGCTGCAACAAATGAAGAAAATGGCGGAGAAGCGTAGCCGTTGGATCTCCCTCGGACACGGAGATTACACCGAGATTCCGTCAGAGAAAGACTTCTTCTCCGTCGTTAAAGCAAGTGATCGTGTTGTTTGTCATTTCTTCCGCGAAAATTGGCCCTGCAAGGTTGTGGACAAGCATTTAAGTATTTTGGCGAAGCAGCATATTGAGACTCGGTTTGTGAAAATAAATGCTGAAAAAAGTCCATTTTTAGCTGAGAAGCTCAAGATCATTGTTCTTCCTACTATTGCTCTCATAAAGAATACCAAAGTGGATGATTATGTGGTGGGTTTTGATGAACTTGGTGGTACTGATGACTTTAGCACCGAGGTTTTGGAAGAAAGATTGGCTAAAGCTCAAGTCATCTTTTTGGAGGGTGAATCATCAATACACCGTGCAAGGTCCGGGGCGCAAACCAAAAGAAGTGTTCGGCAGAGCACAACGGCTGACTCCTCAGATTCTGAGTAA
Protein sequence

>MsG0180005781.01.T01

MDKGKIQEVIENQVLTVAQAVEDKIDEEIAALERLDDDDLEALRERRLQQMKKMAEKRSRWISLGHGDYTEIPSEKDFFSVVKASDRVVCHFFRENWPCKVVDKHLSILAKQHIETRFVKINAEKSPFLAEKLKIIVLPTIALIKNTKVDDYVVGFDELGGTDDFSTEVLEERLAKAQVIFLEGESSIHRARSGAQTKRSVRQSTTADSSDSE*