Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005884.01.T01 | XP_003592583.3 | 96.058 | 482 | 19 | 0 | 1 | 482 | 1 | 482 | 0 | 975 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005884.01.T01 | Q8L3W1 | 32.344 | 337 | 185 | 11 | 18 | 314 | 6 | 339 | 5.68E-42 | 155 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005884.01.T01 | G7IAW3 | 96.000 | 450 | 18 | 0 | 33 | 482 | 12 | 461 | 0.0 | 912 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0780038244.01 | MsG0180005884.01 | PPI |
| MsG0180005884.01 | MsG0780038244.01 | PPI |
| MsG0180005884.01 | MsG0780038248.01 | PPI |
| MsG0180001316.01 | MsG0180005884.01 | PPI |
| MsG0180005884.01 | MsG0180002477.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005884.01.T01 | MTR_1g108780 | 96.000 | 450 | 18 | 0 | 33 | 482 | 12 | 461 | 0.0 | 912 |
| MsG0180005884.01.T01 | MTR_8g103940 | 70.208 | 480 | 134 | 6 | 1 | 480 | 26 | 496 | 0.0 | 687 |
| MsG0180005884.01.T01 | MTR_1g035460 | 46.552 | 406 | 193 | 11 | 34 | 427 | 2 | 395 | 3.61e-106 | 322 |
| MsG0180005884.01.T01 | MTR_3g098790 | 35.691 | 311 | 169 | 7 | 13 | 311 | 8 | 299 | 3.82e-54 | 184 |
| MsG0180005884.01.T01 | MTR_7g050710 | 35.873 | 315 | 165 | 10 | 16 | 311 | 11 | 307 | 5.92e-54 | 184 |
| MsG0180005884.01.T01 | MTR_7g050720 | 35.938 | 320 | 165 | 12 | 18 | 311 | 10 | 315 | 6.13e-52 | 179 |
| MsG0180005884.01.T01 | MTR_7g050590 | 36.149 | 296 | 154 | 11 | 34 | 311 | 2 | 280 | 2.39e-49 | 171 |
| MsG0180005884.01.T01 | MTR_7g050580 | 34.494 | 316 | 147 | 9 | 18 | 311 | 13 | 290 | 2.65e-49 | 171 |
| MsG0180005884.01.T01 | MTR_7g050580 | 23.473 | 311 | 152 | 11 | 222 | 479 | 13 | 290 | 2.64e-11 | 65.1 |
| MsG0180005884.01.T01 | MTR_7g050560 | 32.915 | 319 | 164 | 8 | 10 | 311 | 5 | 290 | 3.74e-49 | 171 |
| MsG0180005884.01.T01 | MTR_3g061370 | 33.439 | 314 | 157 | 8 | 13 | 310 | 4 | 281 | 8.70e-48 | 166 |
| MsG0180005884.01.T01 | MTR_3g061350 | 33.654 | 312 | 153 | 10 | 16 | 310 | 10 | 284 | 1.30e-45 | 160 |
| MsG0180005884.01.T01 | MTR_3g061340 | 32.573 | 307 | 161 | 10 | 12 | 310 | 6 | 274 | 2.54e-45 | 159 |
| MsG0180005884.01.T01 | MTR_3g061340 | 34.884 | 129 | 76 | 4 | 351 | 478 | 153 | 274 | 6.26e-13 | 69.3 |
| MsG0180005884.01.T01 | MTR_5g044670 | 30.178 | 338 | 185 | 11 | 3 | 318 | 2 | 310 | 2.67e-44 | 159 |
| MsG0180005884.01.T01 | MTR_3g061310 | 32.258 | 310 | 161 | 9 | 16 | 310 | 10 | 285 | 8.05e-44 | 156 |
| MsG0180005884.01.T01 | MTR_3g061310 | 29.927 | 274 | 153 | 11 | 234 | 478 | 22 | 285 | 1.43e-16 | 80.5 |
| MsG0180005884.01.T01 | MTR_3g061320 | 31.613 | 310 | 157 | 10 | 16 | 310 | 10 | 279 | 6.19e-42 | 150 |
| MsG0180005884.01.T01 | MTR_3g061320 | 35.965 | 114 | 68 | 3 | 366 | 478 | 170 | 279 | 9.04e-12 | 66.2 |
| MsG0180005884.01.T01 | MTR_3g061330 | 31.769 | 277 | 139 | 8 | 16 | 277 | 10 | 251 | 2.39e-38 | 140 |
| MsG0180005884.01.T01 | MTR_6g061070 | 25.397 | 504 | 316 | 14 | 1 | 478 | 1 | 470 | 1.09e-33 | 132 |
| MsG0180005884.01.T01 | MTR_3g061290 | 40.667 | 150 | 80 | 2 | 16 | 158 | 10 | 157 | 1.87e-32 | 123 |
| MsG0180005884.01.T01 | MTR_4g119990 | 27.500 | 320 | 202 | 11 | 13 | 315 | 10 | 316 | 2.18e-31 | 124 |
| MsG0180005884.01.T01 | MTR_1g035570 | 31.046 | 306 | 166 | 10 | 27 | 315 | 14 | 291 | 2.09e-30 | 122 |
| MsG0180005884.01.T01 | MTR_6g043890 | 26.263 | 297 | 200 | 8 | 14 | 302 | 11 | 296 | 1.89e-29 | 118 |
| MsG0180005884.01.T01 | MTR_1g035620 | 25.105 | 474 | 271 | 19 | 18 | 479 | 9 | 410 | 2.80e-28 | 116 |
| MsG0180005884.01.T01 | MTR_3g005420 | 27.273 | 319 | 166 | 11 | 6 | 312 | 273 | 537 | 2.21e-27 | 115 |
| MsG0180005884.01.T01 | MTR_3g005420 | 31.000 | 100 | 68 | 1 | 18 | 116 | 40 | 139 | 5.18e-11 | 65.1 |
| MsG0180005884.01.T01 | MTR_5g070440 | 26.144 | 306 | 158 | 9 | 18 | 312 | 21 | 269 | 1.25e-26 | 108 |
| MsG0180005884.01.T01 | MTR_7g050640 | 45.833 | 96 | 52 | 0 | 16 | 111 | 11 | 106 | 4.35e-26 | 102 |
| MsG0180005884.01.T01 | MTR_1g035640 | 27.729 | 339 | 198 | 12 | 27 | 355 | 14 | 315 | 2.30e-25 | 108 |
| MsG0180005884.01.T01 | MTR_3g005480 | 25.329 | 304 | 155 | 8 | 18 | 312 | 21 | 261 | 1.65e-24 | 102 |
| MsG0180005884.01.T01 | MTR_3g061220 | 39.024 | 123 | 54 | 3 | 16 | 132 | 10 | 117 | 2.50e-21 | 89.7 |
| MsG0180005884.01.T01 | MTR_1g021400 | 25.378 | 331 | 197 | 12 | 23 | 315 | 36 | 354 | 3.67e-20 | 92.4 |
| MsG0180005884.01.T01 | MTR_3g005460 | 24.204 | 314 | 168 | 8 | 15 | 312 | 259 | 518 | 3.87e-20 | 93.6 |
| MsG0180005884.01.T01 | MTR_3g005460 | 22.027 | 513 | 327 | 17 | 18 | 478 | 25 | 516 | 8.89e-20 | 92.4 |
| MsG0180005884.01.T01 | MTR_1g021330 | 25.905 | 359 | 213 | 13 | 16 | 329 | 24 | 374 | 1.17e-19 | 91.3 |
| MsG0180005884.01.T01 | MTR_4g068320 | 37.963 | 108 | 67 | 0 | 18 | 125 | 6 | 113 | 2.38e-19 | 90.5 |
| MsG0180005884.01.T01 | MTR_4g068320 | 38.931 | 131 | 72 | 4 | 199 | 322 | 270 | 399 | 2.81e-18 | 87.4 |
| MsG0180005884.01.T01 | MTR_1g035520 | 24.917 | 301 | 195 | 8 | 34 | 309 | 88 | 382 | 2.45e-19 | 90.1 |
| MsG0180005884.01.T01 | MTR_1g035490 | 25.952 | 289 | 170 | 9 | 202 | 482 | 79 | 331 | 5.75e-19 | 88.2 |
| MsG0180005884.01.T01 | MTR_1g021435 | 25.074 | 339 | 200 | 13 | 23 | 315 | 28 | 358 | 1.50e-18 | 87.4 |
| MsG0180005884.01.T01 | MTR_1g050845 | 25.424 | 295 | 179 | 7 | 18 | 309 | 9 | 265 | 1.82e-18 | 89.0 |
| MsG0180005884.01.T01 | MTR_1g021360 | 25.887 | 282 | 176 | 8 | 32 | 299 | 196 | 458 | 1.36e-17 | 85.5 |
| MsG0180005884.01.T01 | MTR_1g034240 | 35.036 | 137 | 74 | 3 | 18 | 139 | 7 | 143 | 4.27e-17 | 84.0 |
| MsG0180005884.01.T01 | MTR_1g034240 | 36.434 | 129 | 72 | 5 | 199 | 319 | 274 | 400 | 2.30e-15 | 78.6 |
| MsG0180005884.01.T01 | MTR_3g005490 | 26.966 | 178 | 114 | 3 | 18 | 179 | 21 | 198 | 6.89e-17 | 80.9 |
| MsG0180005884.01.T01 | MTR_1g021380 | 22.687 | 335 | 204 | 11 | 16 | 315 | 20 | 334 | 7.60e-17 | 82.4 |
| MsG0180005884.01.T01 | MTR_1g098450 | 26.647 | 334 | 192 | 16 | 8 | 325 | 2 | 298 | 2.18e-16 | 80.5 |
| MsG0180005884.01.T01 | MTR_1g035650 | 35.000 | 100 | 63 | 1 | 17 | 116 | 8 | 105 | 8.17e-16 | 76.6 |
| MsG0180005884.01.T01 | MTR_1g033740 | 34.746 | 118 | 74 | 2 | 13 | 130 | 17 | 131 | 1.15e-15 | 75.9 |
| MsG0180005884.01.T01 | MTR_3g005495 | 25.685 | 292 | 141 | 9 | 18 | 302 | 243 | 465 | 4.46e-15 | 77.8 |
| MsG0180005884.01.T01 | MTR_3g005495 | 33.333 | 84 | 56 | 0 | 33 | 116 | 28 | 111 | 2.56e-12 | 69.3 |
| MsG0180005884.01.T01 | MTR_3g061300 | 37.288 | 118 | 69 | 3 | 366 | 482 | 14 | 127 | 5.11e-15 | 72.0 |
| MsG0180005884.01.T01 | MTR_1g034210 | 34.746 | 118 | 68 | 4 | 209 | 319 | 251 | 366 | 2.88e-14 | 74.7 |
| MsG0180005884.01.T01 | MTR_1g034210 | 35.354 | 99 | 62 | 1 | 18 | 116 | 7 | 103 | 3.97e-12 | 68.2 |
| MsG0180005884.01.T01 | MTR_1g035500 | 33.898 | 118 | 75 | 2 | 13 | 130 | 20 | 134 | 3.53e-14 | 71.2 |
| MsG0180005884.01.T01 | MTR_1g035480 | 33.613 | 119 | 76 | 2 | 12 | 130 | 11 | 126 | 3.87e-14 | 71.2 |
| MsG0180005884.01.T01 | MTR_1g035440 | 33.010 | 103 | 68 | 1 | 15 | 116 | 12 | 114 | 2.70e-13 | 70.1 |
| MsG0180005884.01.T01 | MTR_1g035470 | 42.169 | 83 | 45 | 2 | 34 | 116 | 2 | 81 | 1.10e-12 | 65.9 |
| MsG0180005884.01.T01 | MTR_1g021290 | 33.333 | 129 | 72 | 3 | 19 | 134 | 157 | 284 | 2.12e-12 | 68.6 |
| MsG0180005884.01.T01 | MTR_1g034210 | 35.354 | 99 | 62 | 1 | 18 | 116 | 7 | 103 | 2.85e-12 | 68.2 |
| MsG0180005884.01.T01 | MTR_3g005440 | 29.221 | 154 | 100 | 3 | 10 | 159 | 230 | 378 | 3.35e-11 | 65.5 |
| MsG0180005884.01.T01 | MTR_3g005440 | 31.765 | 85 | 58 | 0 | 32 | 116 | 34 | 118 | 3.38e-11 | 65.5 |
| MsG0180005884.01.T01 | MTR_3g005420 | 31.000 | 100 | 68 | 1 | 18 | 116 | 21 | 120 | 4.92e-11 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005884.01.T01 | AT3G18990 | 32.344 | 337 | 185 | 11 | 18 | 314 | 6 | 339 | 5.79e-43 | 155 |
| MsG0180005884.01.T01 | AT3G18990 | 32.047 | 337 | 186 | 11 | 18 | 314 | 6 | 339 | 3.96e-42 | 154 |
| MsG0180005884.01.T01 | AT1G49480 | 31.977 | 172 | 88 | 7 | 169 | 314 | 56 | 224 | 2.49e-17 | 81.6 |
| MsG0180005884.01.T01 | AT1G49480 | 35.897 | 117 | 66 | 4 | 368 | 479 | 109 | 221 | 5.38e-11 | 62.8 |
| MsG0180005884.01.T01 | AT1G49480 | 31.977 | 172 | 88 | 7 | 169 | 314 | 56 | 224 | 2.49e-17 | 81.6 |
| MsG0180005884.01.T01 | AT1G49480 | 35.897 | 117 | 66 | 4 | 368 | 479 | 109 | 221 | 5.38e-11 | 62.8 |
| MsG0180005884.01.T01 | AT1G49480 | 31.977 | 172 | 88 | 7 | 169 | 314 | 56 | 224 | 2.49e-17 | 81.6 |
| MsG0180005884.01.T01 | AT1G49480 | 35.897 | 117 | 66 | 4 | 368 | 479 | 109 | 221 | 5.38e-11 | 62.8 |
| MsG0180005884.01.T01 | AT4G01580 | 36.538 | 104 | 66 | 0 | 17 | 120 | 29 | 132 | 9.74e-17 | 79.0 |
| MsG0180005884.01.T01 | AT4G33280 | 26.667 | 300 | 197 | 10 | 32 | 318 | 37 | 326 | 2.73e-16 | 80.5 |
| MsG0180005884.01.T01 | AT3G18960 | 34.615 | 104 | 68 | 0 | 17 | 120 | 29 | 132 | 6.04e-16 | 76.6 |
| MsG0180005884.01.T01 | AT3G18960 | 34.615 | 104 | 68 | 0 | 17 | 120 | 29 | 132 | 9.88e-16 | 76.3 |
| MsG0180005884.01.T01 | AT1G49475 | 31.667 | 120 | 77 | 2 | 17 | 136 | 33 | 147 | 2.03e-14 | 72.0 |
| MsG0180005884.01.T01 | AT3G18960 | 35.227 | 88 | 57 | 0 | 33 | 120 | 8 | 95 | 1.73e-11 | 63.2 |
Find 78 sgRNAs with CRISPR-Local
Find 134 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAACAATCCAATTGAAATTT+TGG | 0.104888 | 1:+97119245 | MsG0180005884.01.T01:CDS |
| CCTAAACTTGTCCAAGTTAA+AGG | 0.118978 | 1:+97119390 | MsG0180005884.01.T01:CDS |
| ATCTGATAATAATTTGAAAT+TGG | 0.143719 | 1:+97119940 | MsG0180005884.01.T01:CDS |
| CTCAAATTGATAGTGAAATT+TGG | 0.167485 | 1:-97120614 | None:intergenic |
| GAACTTGAAATTTGTGATTT+TGG | 0.194976 | 1:-97119297 | None:intergenic |
| GTGAGACTGTACATATTCTT+AGG | 0.199034 | 1:-97118534 | None:intergenic |
| GGAAATACATTTGACTAAAA+AGG | 0.231817 | 1:+97119047 | MsG0180005884.01.T01:CDS |
| CTTGACCATGCTTCACATTC+AGG | 0.239372 | 1:-97119543 | None:intergenic |
| TATCATGACAGCAGTGAAAA+AGG | 0.253877 | 1:-97119635 | None:intergenic |
| CCTTCACCCAACTTTGTTGC+TGG | 0.270614 | 1:-97120935 | None:intergenic |
| CCTTTAACTTGGACAAGTTT+AGG | 0.279816 | 1:-97119390 | None:intergenic |
| TGCTATTGATCATCTCAAAA+AGG | 0.283820 | 1:-97119973 | None:intergenic |
| ACCCAACTTTGTTGCTGGTT+CGG | 0.316172 | 1:-97120930 | None:intergenic |
| CGAAATCGCCCTTCAGGTCT+TGG | 0.340066 | 1:+97119850 | MsG0180005884.01.T01:CDS |
| TCAATTTGAGATCAAGTCAT+TGG | 0.345291 | 1:+97120627 | MsG0180005884.01.T01:CDS |
| TGCTCATCCAAAATTTCAAT+TGG | 0.347442 | 1:-97119252 | None:intergenic |
| TATTTCTCAGTGAAAGCATT+TGG | 0.349857 | 1:-97118970 | None:intergenic |
| TCTGATAATAATTTGAAATT+GGG | 0.355411 | 1:+97119941 | MsG0180005884.01.T01:CDS |
| GTACATATTCTTAGGGTTGC+TGG | 0.358212 | 1:-97118526 | None:intergenic |
| GACTGCAATGTTACAGTTTG+AGG | 0.362858 | 1:+97120871 | MsG0180005884.01.T01:CDS |
| TTCATTCTCGAGCAAGTACT+TGG | 0.373540 | 1:+97120835 | MsG0180005884.01.T01:CDS |
| AAAACTATTATGTCGTATAA+AGG | 0.388718 | 1:+97120205 | MsG0180005884.01.T01:CDS |
| GCAAAGCGAAATCGCCCTTC+AGG | 0.399129 | 1:+97119844 | MsG0180005884.01.T01:CDS |
| GAAATTGAATATCGTGTTGA+CGG | 0.403003 | 1:+97119204 | MsG0180005884.01.T01:CDS |
| TGTAGAAGAAAATAAATTGC+AGG | 0.404615 | 1:+97120970 | MsG0180005884.01.T01:CDS |
| AAGCACAACATCTTCCTTGT+TGG | 0.409928 | 1:-97121020 | None:intergenic |
| CAAAGTGGATGTGAATTGAT+TGG | 0.417163 | 1:-97120585 | None:intergenic |
| CTTCACATTCAGGCATTTAG+TGG | 0.427188 | 1:-97119533 | None:intergenic |
| ATATTTGGATTCAAAGGGGT+TGG | 0.428451 | 1:+97119076 | MsG0180005884.01.T01:CDS |
| ATTTCCCATTCTTTCTCATC+GGG | 0.433534 | 1:-97119030 | None:intergenic |
| ATGGTGATATTTGGATTCAA+AGG | 0.444319 | 1:+97119070 | MsG0180005884.01.T01:CDS |
| AATTTATTATACCTTTAACT+TGG | 0.446187 | 1:-97119401 | None:intergenic |
| AACTTTGTTGCTGGTTCGGA+TGG | 0.454779 | 1:-97120926 | None:intergenic |
| TCCGAACCAGCAACAAAGTT+GGG | 0.458098 | 1:+97120929 | MsG0180005884.01.T01:CDS |
| TGGTGATATTTGGATTCAAA+GGG | 0.458234 | 1:+97119071 | MsG0180005884.01.T01:CDS |
| GGTCTACTCTTCTTGTGAGA+AGG | 0.460496 | 1:-97119276 | None:intergenic |
| ATTCTTAGGGTTGCTGGTCA+TGG | 0.460769 | 1:-97118520 | None:intergenic |
| TTGGATTCAAAGGGGTTGGA+AGG | 0.464926 | 1:+97119080 | MsG0180005884.01.T01:CDS |
| AGACTCCCGATGAGAAAGAA+TGG | 0.468520 | 1:+97119025 | MsG0180005884.01.T01:CDS |
| CAATCAATTCACATCCACTT+TGG | 0.477065 | 1:+97120586 | MsG0180005884.01.T01:CDS |
| TCTTGGATGATGAGAGAACT+TGG | 0.477954 | 1:+97119867 | MsG0180005884.01.T01:CDS |
| TTTGAATCAGTTCATGGAGA+TGG | 0.482390 | 1:+97120139 | MsG0180005884.01.T01:intron |
| ACACATTATTCATTAGATCA+TGG | 0.484576 | 1:+97119111 | MsG0180005884.01.T01:CDS |
| TTCATGGAGATGGAATTAAC+TGG | 0.486836 | 1:+97120149 | MsG0180005884.01.T01:CDS |
| TATTTCCCATTCTTTCTCAT+CGG | 0.492052 | 1:-97119031 | None:intergenic |
| CAATTTGAGATCAAGTCATT+GGG | 0.498692 | 1:+97120628 | MsG0180005884.01.T01:CDS |
| TGAGACTGTACATATTCTTA+GGG | 0.500244 | 1:-97118533 | None:intergenic |
| GACTCCCGATGAGAAAGAAT+GGG | 0.507920 | 1:+97119026 | MsG0180005884.01.T01:CDS |
| TTGGATTACCTTGTGGTGGA+AGG | 0.508508 | 1:-97120048 | None:intergenic |
| ATCCGAACCAGCAACAAAGT+TGG | 0.508654 | 1:+97120928 | MsG0180005884.01.T01:CDS |
| GGATAATACATCACTTGTAC+AGG | 0.514406 | 1:-97120905 | None:intergenic |
| ACCTCAACAAATATTCAAGA+TGG | 0.526205 | 1:+97118588 | MsG0180005884.01.T01:CDS |
| AATTAGTACTTTAAAAGCAT+AGG | 0.530987 | 1:-97119999 | None:intergenic |
| GCTTTCACTGAGAAATATAG+TGG | 0.532968 | 1:+97118976 | MsG0180005884.01.T01:CDS |
| TCTCATCATCCAAGACCTGA+AGG | 0.534348 | 1:-97119859 | None:intergenic |
| CAACAAAGTTGGGTGAAGGT+TGG | 0.542982 | 1:+97120939 | MsG0180005884.01.T01:CDS |
| TGGATTACCTTGTGGTGGAA+GGG | 0.545050 | 1:-97120047 | None:intergenic |
| GAAGAAAATAAATTGCAGGC+TGG | 0.548366 | 1:+97120974 | MsG0180005884.01.T01:CDS |
| TCGGGAGTCTTGAGAAACAT+TGG | 0.550403 | 1:-97119012 | None:intergenic |
| AACTCAAAGAAATTCACTGC+AGG | 0.555404 | 1:+97120520 | MsG0180005884.01.T01:CDS |
| ATTAACTGGTTAGAAACTGC+AGG | 0.556059 | 1:+97120163 | MsG0180005884.01.T01:CDS |
| AAAGAAATTCACTGCAGGCT+AGG | 0.559813 | 1:+97120525 | MsG0180005884.01.T01:CDS |
| TCCATCTTGAATATTTGTTG+AGG | 0.562475 | 1:-97118589 | None:intergenic |
| TTCATTAGATCATGGTCACA+TGG | 0.571925 | 1:+97119119 | MsG0180005884.01.T01:CDS |
| CATCTTGGATTACCTTGTGG+TGG | 0.585751 | 1:-97120052 | None:intergenic |
| TCATTGGGATGTTTACAGAC+CGG | 0.600053 | 1:+97120643 | MsG0180005884.01.T01:CDS |
| GGTGATATTTGGATTCAAAG+GGG | 0.604343 | 1:+97119072 | MsG0180005884.01.T01:CDS |
| TTCATATGTTAATCAATACA+TGG | 0.607228 | 1:+97119660 | MsG0180005884.01.T01:CDS |
| CTCATCATCCAAGACCTGAA+GGG | 0.610171 | 1:-97119858 | None:intergenic |
| ATTGACACAAGTGAAGATGT+CGG | 0.614793 | 1:+97119342 | MsG0180005884.01.T01:CDS |
| TATGTAAACCTCAAAATGAG+AGG | 0.618304 | 1:-97119163 | None:intergenic |
| CCAGCAACAAAGTTGGGTGA+AGG | 0.622086 | 1:+97120935 | MsG0180005884.01.T01:CDS |
| ACGGTAATAATCAACATGAA+CGG | 0.624679 | 1:+97119223 | MsG0180005884.01.T01:CDS |
| TCATTCTCGAGCAAGTACTT+GGG | 0.630141 | 1:+97120836 | MsG0180005884.01.T01:CDS |
| CAACATTCCCTTCCACCACA+AGG | 0.632695 | 1:+97120040 | MsG0180005884.01.T01:CDS |
| TGAAGCATGGTCAAGAGCAG+AGG | 0.641697 | 1:+97119551 | MsG0180005884.01.T01:CDS |
| TTGGCATACCTCAGGAACGA+AGG | 0.641759 | 1:+97120496 | MsG0180005884.01.T01:intron |
| AAATGCCTGAATGTGAAGCA+TGG | 0.702749 | 1:+97119538 | MsG0180005884.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGATTACTAATAACTTTA+AGG | - | Chr1:97120085-97120104 | None:intergenic | 15.0% |
| !! | AATTTATTATACCTTTAACT+TGG | - | Chr1:97119404-97119423 | None:intergenic | 15.0% |
| !!! | ACTTTTGTTCAAAATTTAAA+GGG | - | Chr1:97119811-97119830 | None:intergenic | 15.0% |
| !!! | ATCTGATAATAATTTGAAAT+TGG | + | Chr1:97119940-97119959 | MsG0180005884.01.T01:CDS | 15.0% |
| !!! | TAAAGTTATTAGTAATCTTT+TGG | + | Chr1:97120085-97120104 | MsG0180005884.01.T01:intron | 15.0% |
| !!! | TAATACAAAATGTTGTTTAA+TGG | + | Chr1:97120337-97120356 | MsG0180005884.01.T01:intron | 15.0% |
| !!! | TACTTTTGTTCAAAATTTAA+AGG | - | Chr1:97119812-97119831 | None:intergenic | 15.0% |
| !!! | TCTGATAATAATTTGAAATT+GGG | + | Chr1:97119941-97119960 | MsG0180005884.01.T01:CDS | 15.0% |
| !! | AAAACTATTATGTCGTATAA+AGG | + | Chr1:97120205-97120224 | MsG0180005884.01.T01:CDS | 20.0% |
| !! | AATCACTAGATATATGTATT+TGG | - | Chr1:97120697-97120716 | None:intergenic | 20.0% |
| !! | AGGTATAATAAATTCATGAT+TGG | + | Chr1:97119410-97119429 | MsG0180005884.01.T01:intron | 20.0% |
| !! | ATCATACAGTACTTAAATTT+CGG | - | Chr1:97118680-97118699 | None:intergenic | 20.0% |
| !! | TTCATATGTTAATCAATACA+TGG | + | Chr1:97119660-97119679 | MsG0180005884.01.T01:CDS | 20.0% |
| !!! | AATTAGTACTTTAAAAGCAT+AGG | - | Chr1:97120002-97120021 | None:intergenic | 20.0% |
| !!! | AATTGATTATATGCTTTTGT+TGG | + | Chr1:97120477-97120496 | MsG0180005884.01.T01:intron | 20.0% |
| !!! | TCAATTCTCAATTTTTTAAG+TGG | - | Chr1:97119327-97119346 | None:intergenic | 20.0% |
| !!! | TTTTCTTAGAAAGATACATA+TGG | - | Chr1:97118799-97118818 | None:intergenic | 20.0% |
| !!! | TTTTGAACAAAAGTATTTGA+AGG | + | Chr1:97119817-97119836 | MsG0180005884.01.T01:CDS | 20.0% |
| ! | AAGAAAGAAAGAAAGAAAGA+AGG | - | Chr1:97118634-97118653 | None:intergenic | 25.0% |
| ! | ACACATTATTCATTAGATCA+TGG | + | Chr1:97119111-97119130 | MsG0180005884.01.T01:CDS | 25.0% |
| ! | ATACATTTGACTAAAAAGGA+TGG | + | Chr1:97119051-97119070 | MsG0180005884.01.T01:CDS | 25.0% |
| ! | ATTAAAAGTTCAAGTGAGTT+AGG | - | Chr1:97118757-97118776 | None:intergenic | 25.0% |
| ! | CATAAATACATATTCCTTAC+CGG | - | Chr1:97120665-97120684 | None:intergenic | 25.0% |
| ! | GGAAATACATTTGACTAAAA+AGG | + | Chr1:97119047-97119066 | MsG0180005884.01.T01:CDS | 25.0% |
| ! | TGTAGAAGAAAATAAATTGC+AGG | + | Chr1:97120970-97120989 | MsG0180005884.01.T01:CDS | 25.0% |
| ! | TGTATGAGAAAAATTAGTAG+CGG | + | Chr1:97119902-97119921 | MsG0180005884.01.T01:CDS | 25.0% |
| !! | GAACAATCCAATTGAAATTT+TGG | + | Chr1:97119245-97119264 | MsG0180005884.01.T01:CDS | 25.0% |
| !! | GAACTTGAAATTTGTGATTT+TGG | - | Chr1:97119300-97119319 | None:intergenic | 25.0% |
| !! | GTTTCTTTTACAGTTAGTTA+GGG | - | Chr1:97120790-97120809 | None:intergenic | 25.0% |
| !! | TGTTTCTTTTACAGTTAGTT+AGG | - | Chr1:97120791-97120810 | None:intergenic | 25.0% |
| !! | TTGTTATGTTATGTTTTACG+CGG | + | Chr1:97118706-97118725 | MsG0180005884.01.T01:intron | 25.0% |
| !!! | CTCAAATTGATAGTGAAATT+TGG | - | Chr1:97120617-97120636 | None:intergenic | 25.0% |
| ACCTCAACAAATATTCAAGA+TGG | + | Chr1:97118588-97118607 | MsG0180005884.01.T01:CDS | 30.0% | |
| ACGGTAATAATCAACATGAA+CGG | + | Chr1:97119223-97119242 | MsG0180005884.01.T01:CDS | 30.0% | |
| ATGGTGATATTTGGATTCAA+AGG | + | Chr1:97119070-97119089 | MsG0180005884.01.T01:CDS | 30.0% | |
| ATTCAACTTCAAATTCCCAA+AGG | - | Chr1:97120258-97120277 | None:intergenic | 30.0% | |
| CAAAATTTAAAGGGACATAC+TGG | - | Chr1:97119802-97119821 | None:intergenic | 30.0% | |
| CTAAAAAGGATGGTGATATT+TGG | + | Chr1:97119061-97119080 | MsG0180005884.01.T01:CDS | 30.0% | |
| CTAGTGATTATGCTTAAAAC+TGG | + | Chr1:97120708-97120727 | MsG0180005884.01.T01:intron | 30.0% | |
| GAAATTGAATATCGTGTTGA+CGG | + | Chr1:97119204-97119223 | MsG0180005884.01.T01:CDS | 30.0% | |
| TACAGTTTGAGGAAAAATTG+TGG | + | Chr1:97120882-97120901 | MsG0180005884.01.T01:CDS | 30.0% | |
| TATGTAAACCTCAAAATGAG+AGG | - | Chr1:97119166-97119185 | None:intergenic | 30.0% | |
| TATTTCCCATTCTTTCTCAT+CGG | - | Chr1:97119034-97119053 | None:intergenic | 30.0% | |
| TGAGACTGTACATATTCTTA+GGG | - | Chr1:97118536-97118555 | None:intergenic | 30.0% | |
| TGCTATTGATCATCTCAAAA+AGG | - | Chr1:97119976-97119995 | None:intergenic | 30.0% | |
| TGCTCATCCAAAATTTCAAT+TGG | - | Chr1:97119255-97119274 | None:intergenic | 30.0% | |
| TGGTGATATTTGGATTCAAA+GGG | + | Chr1:97119071-97119090 | MsG0180005884.01.T01:CDS | 30.0% | |
| TTCAACTTCAAATTCCCAAA+GGG | - | Chr1:97120257-97120276 | None:intergenic | 30.0% | |
| ! | AACATTGCAGTCTTCTTTTT+GGG | - | Chr1:97120863-97120882 | None:intergenic | 30.0% |
| ! | CAATTTGAGATCAAGTCATT+GGG | + | Chr1:97120628-97120647 | MsG0180005884.01.T01:CDS | 30.0% |
| ! | CTCTTCTGAGAAATTGAATA+TGG | - | Chr1:97119503-97119522 | None:intergenic | 30.0% |
| ! | CTTAGCTTCAGAATTTTTGA+AGG | - | Chr1:97120558-97120577 | None:intergenic | 30.0% |
| ! | CTTGATGTTCATATTTTCAG+AGG | + | Chr1:97121040-97121059 | MsG0180005884.01.T01:CDS | 30.0% |
| ! | CTTTAAGGAAAAAACCATCT+TGG | - | Chr1:97120070-97120089 | None:intergenic | 30.0% |
| ! | TAACATTGCAGTCTTCTTTT+TGG | - | Chr1:97120864-97120883 | None:intergenic | 30.0% |
| ! | TCAATTTGAGATCAAGTCAT+TGG | + | Chr1:97120627-97120646 | MsG0180005884.01.T01:CDS | 30.0% |
| ! | TCCATCTTGAATATTTGTTG+AGG | - | Chr1:97118592-97118611 | None:intergenic | 30.0% |
| ! | TTTAGGAATGTTTTGCGATT+TGG | - | Chr1:97119376-97119395 | None:intergenic | 30.0% |
| !! | TATTTCTCAGTGAAAGCATT+TGG | - | Chr1:97118973-97118992 | None:intergenic | 30.0% |
| !!! | GAAATTTGGTTTTTCCAAAG+TGG | - | Chr1:97120603-97120622 | None:intergenic | 30.0% |
| AACTCAAAGAAATTCACTGC+AGG | + | Chr1:97120520-97120539 | MsG0180005884.01.T01:CDS | 35.0% | |
| AGAAGGAAAGAATGCTTACA+AGG | - | Chr1:97118617-97118636 | None:intergenic | 35.0% | |
| ATATTTGGATTCAAAGGGGT+TGG | + | Chr1:97119076-97119095 | MsG0180005884.01.T01:CDS | 35.0% | |
| ATCAAGCAGTATTGTCAAGA+TGG | + | Chr1:97120276-97120295 | MsG0180005884.01.T01:intron | 35.0% | |
| ATCATGACAGCAGTGAAAAA+GGG | - | Chr1:97119637-97119656 | None:intergenic | 35.0% | |
| ATTAACTGGTTAGAAACTGC+AGG | + | Chr1:97120163-97120182 | MsG0180005884.01.T01:CDS | 35.0% | |
| ATTGACACAAGTGAAGATGT+CGG | + | Chr1:97119342-97119361 | MsG0180005884.01.T01:CDS | 35.0% | |
| ATTTCCCATTCTTTCTCATC+GGG | - | Chr1:97119033-97119052 | None:intergenic | 35.0% | |
| CAAAGTGGATGTGAATTGAT+TGG | - | Chr1:97120588-97120607 | None:intergenic | 35.0% | |
| CAATCAATTCACATCCACTT+TGG | + | Chr1:97120586-97120605 | MsG0180005884.01.T01:CDS | 35.0% | |
| CCTAAACTTGTCCAAGTTAA+AGG | + | Chr1:97119390-97119409 | MsG0180005884.01.T01:CDS | 35.0% | |
| CGTGAGTCGTAAAAAAATAG+AGG | - | Chr1:97120386-97120405 | None:intergenic | 35.0% | |
| GAAGAAAATAAATTGCAGGC+TGG | + | Chr1:97120974-97120993 | MsG0180005884.01.T01:CDS | 35.0% | |
| GAAGGAAAGAATGCTTACAA+GGG | - | Chr1:97118616-97118635 | None:intergenic | 35.0% | |
| GATTATGCTTAAAACTGGAG+TGG | + | Chr1:97120713-97120732 | MsG0180005884.01.T01:intron | 35.0% | |
| GCTTTCACTGAGAAATATAG+TGG | + | Chr1:97118976-97118995 | MsG0180005884.01.T01:CDS | 35.0% | |
| GGATAATACATCACTTGTAC+AGG | - | Chr1:97120908-97120927 | None:intergenic | 35.0% | |
| GGTGATATTTGGATTCAAAG+GGG | + | Chr1:97119072-97119091 | MsG0180005884.01.T01:CDS | 35.0% | |
| GTGAGACTGTACATATTCTT+AGG | - | Chr1:97118537-97118556 | None:intergenic | 35.0% | |
| TATCATGACAGCAGTGAAAA+AGG | - | Chr1:97119638-97119657 | None:intergenic | 35.0% | |
| TCATCCTTTGAATCAGTTCA+TGG | + | Chr1:97120133-97120152 | MsG0180005884.01.T01:intron | 35.0% | |
| TTCATGGAGATGGAATTAAC+TGG | + | Chr1:97120149-97120168 | MsG0180005884.01.T01:CDS | 35.0% | |
| TTCATTAGATCATGGTCACA+TGG | + | Chr1:97119119-97119138 | MsG0180005884.01.T01:CDS | 35.0% | |
| TTTGAATCAGTTCATGGAGA+TGG | + | Chr1:97120139-97120158 | MsG0180005884.01.T01:intron | 35.0% | |
| ! | ATCTCCATGAACTGATTCAA+AGG | - | Chr1:97120140-97120159 | None:intergenic | 35.0% |
| ! | TCAGAAAACCTCTCATTTTG+AGG | + | Chr1:97119155-97119174 | MsG0180005884.01.T01:CDS | 35.0% |
| ! | TGAGAAAAATTAGTAGCGGA+TGG | + | Chr1:97119906-97119925 | MsG0180005884.01.T01:CDS | 35.0% |
| !! | CCTTTAACTTGGACAAGTTT+AGG | - | Chr1:97119393-97119412 | None:intergenic | 35.0% |
| !! | TCAGAATTTTTGAAGGAGCA+TGG | - | Chr1:97120551-97120570 | None:intergenic | 35.0% |
| AAAGAAATTCACTGCAGGCT+AGG | + | Chr1:97120525-97120544 | MsG0180005884.01.T01:CDS | 40.0% | |
| AAATGCCTGAATGTGAAGCA+TGG | + | Chr1:97119538-97119557 | MsG0180005884.01.T01:CDS | 40.0% | |
| AACCATCTTGGATTACCTTG+TGG | - | Chr1:97120058-97120077 | None:intergenic | 40.0% | |
| AAGCACAACATCTTCCTTGT+TGG | - | Chr1:97121023-97121042 | None:intergenic | 40.0% | |
| CTTCACATTCAGGCATTTAG+TGG | - | Chr1:97119536-97119555 | None:intergenic | 40.0% | |
| GACTGCAATGTTACAGTTTG+AGG | + | Chr1:97120871-97120890 | MsG0180005884.01.T01:CDS | 40.0% | |
| GTACATATTCTTAGGGTTGC+TGG | - | Chr1:97118529-97118548 | None:intergenic | 40.0% | |
| TCATTCTCGAGCAAGTACTT+GGG | + | Chr1:97120836-97120855 | MsG0180005884.01.T01:CDS | 40.0% | |
| TCATTGGGATGTTTACAGAC+CGG | + | Chr1:97120643-97120662 | MsG0180005884.01.T01:CDS | 40.0% | |
| TCTTGGATGATGAGAGAACT+TGG | + | Chr1:97119867-97119886 | MsG0180005884.01.T01:CDS | 40.0% | |
| TTCATTCTCGAGCAAGTACT+TGG | + | Chr1:97120835-97120854 | MsG0180005884.01.T01:CDS | 40.0% | |
| !! | TGTTTTTGTGCTTGCCAACA+AGG | + | Chr1:97121006-97121025 | MsG0180005884.01.T01:CDS | 40.0% |
| AGACTCCCGATGAGAAAGAA+TGG | + | Chr1:97119025-97119044 | MsG0180005884.01.T01:CDS | 45.0% | |
| AGTTAGGTCCTGAGGATACA+AGG | - | Chr1:97118741-97118760 | None:intergenic | 45.0% | |
| ATCCGAACCAGCAACAAAGT+TGG | + | Chr1:97120928-97120947 | MsG0180005884.01.T01:CDS | 45.0% | |
| ATCGTTGTTGTGACTCCCTT+TGG | + | Chr1:97120239-97120258 | MsG0180005884.01.T01:intron | 45.0% | |
| CAACAAAGTTGGGTGAAGGT+TGG | + | Chr1:97120939-97120958 | MsG0180005884.01.T01:CDS | 45.0% | |
| CACCACAAGGTAATCCAAGA+TGG | + | Chr1:97120053-97120072 | MsG0180005884.01.T01:intron | 45.0% | |
| CATCTTGGATTACCTTGTGG+TGG | - | Chr1:97120055-97120074 | None:intergenic | 45.0% | |
| CTCATCATCCAAGACCTGAA+GGG | - | Chr1:97119861-97119880 | None:intergenic | 45.0% | |
| CTTGACCATGCTTCACATTC+AGG | - | Chr1:97119546-97119565 | None:intergenic | 45.0% | |
| GACTCCCGATGAGAAAGAAT+GGG | + | Chr1:97119026-97119045 | MsG0180005884.01.T01:CDS | 45.0% | |
| GATTCCTTCCTTGTATCCTC+AGG | + | Chr1:97118730-97118749 | MsG0180005884.01.T01:intron | 45.0% | |
| GGTCTACTCTTCTTGTGAGA+AGG | - | Chr1:97119279-97119298 | None:intergenic | 45.0% | |
| TCCGAACCAGCAACAAAGTT+GGG | + | Chr1:97120929-97120948 | MsG0180005884.01.T01:CDS | 45.0% | |
| TCGGGAGTCTTGAGAAACAT+TGG | - | Chr1:97119015-97119034 | None:intergenic | 45.0% | |
| TCGTTGTTGTGACTCCCTTT+GGG | + | Chr1:97120240-97120259 | MsG0180005884.01.T01:intron | 45.0% | |
| TCTCATCATCCAAGACCTGA+AGG | - | Chr1:97119862-97119881 | None:intergenic | 45.0% | |
| TTCAAGTGAGTTAGGTCCTG+AGG | - | Chr1:97118749-97118768 | None:intergenic | 45.0% | |
| TTGAGTTTCCTTCGTTCCTG+AGG | - | Chr1:97120507-97120526 | None:intergenic | 45.0% | |
| TTGGATTCAAAGGGGTTGGA+AGG | + | Chr1:97119080-97119099 | MsG0180005884.01.T01:CDS | 45.0% | |
| ! | ACCCAACTTTGTTGCTGGTT+CGG | - | Chr1:97120933-97120952 | None:intergenic | 45.0% |
| ! | TGCTTTTGTTGGCATACCTC+AGG | + | Chr1:97120488-97120507 | MsG0180005884.01.T01:intron | 45.0% |
| ! | TGGATTACCTTGTGGTGGAA+GGG | - | Chr1:97120050-97120069 | None:intergenic | 45.0% |
| ! | TTGGATTACCTTGTGGTGGA+AGG | - | Chr1:97120051-97120070 | None:intergenic | 45.0% |
| !! | AACTTTGTTGCTGGTTCGGA+TGG | - | Chr1:97120929-97120948 | None:intergenic | 45.0% |
| AGGTCCTGAGGATACAAGGA+AGG | - | Chr1:97118737-97118756 | None:intergenic | 50.0% | |
| CAACATTCCCTTCCACCACA+AGG | + | Chr1:97120040-97120059 | MsG0180005884.01.T01:CDS | 50.0% | |
| CCAGCAACAAAGTTGGGTGA+AGG | + | Chr1:97120935-97120954 | MsG0180005884.01.T01:CDS | 50.0% | |
| CCTTCACCCAACTTTGTTGC+TGG | - | Chr1:97120938-97120957 | None:intergenic | 50.0% | |
| GGGATGTTTACAGACCGGTA+AGG | + | Chr1:97120648-97120667 | MsG0180005884.01.T01:intron | 50.0% | |
| TGAAGCATGGTCAAGAGCAG+AGG | + | Chr1:97119551-97119570 | MsG0180005884.01.T01:CDS | 50.0% | |
| TTGGCATACCTCAGGAACGA+AGG | + | Chr1:97120496-97120515 | MsG0180005884.01.T01:intron | 50.0% | |
| CGAAATCGCCCTTCAGGTCT+TGG | + | Chr1:97119850-97119869 | MsG0180005884.01.T01:CDS | 55.0% | |
| GCAAAGCGAAATCGCCCTTC+AGG | + | Chr1:97119844-97119863 | MsG0180005884.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 97118522 | 97121072 | 97118522 | ID=MsG0180005884.01;Name=MsG0180005884.01 |
| Chr1 | mRNA | 97118522 | 97121072 | 97118522 | ID=MsG0180005884.01.T01;Parent=MsG0180005884.01;Name=MsG0180005884.01.T01;_AED=0.17;_eAED=0.17;_QI=0|0|0|1|1|1|7|0|482 |
| Chr1 | exon | 97118522 | 97118617 | 97118522 | ID=MsG0180005884.01.T01:exon:10751;Parent=MsG0180005884.01.T01 |
| Chr1 | exon | 97118964 | 97119411 | 97118964 | ID=MsG0180005884.01.T01:exon:10752;Parent=MsG0180005884.01.T01 |
| Chr1 | exon | 97119518 | 97119681 | 97119518 | ID=MsG0180005884.01.T01:exon:10753;Parent=MsG0180005884.01.T01 |
| Chr1 | exon | 97119803 | 97120061 | 97119803 | ID=MsG0180005884.01.T01:exon:10754;Parent=MsG0180005884.01.T01 |
| Chr1 | exon | 97120149 | 97120226 | 97120149 | ID=MsG0180005884.01.T01:exon:10755;Parent=MsG0180005884.01.T01 |
| Chr1 | exon | 97120510 | 97120664 | 97120510 | ID=MsG0180005884.01.T01:exon:10756;Parent=MsG0180005884.01.T01 |
| Chr1 | exon | 97120824 | 97121072 | 97120824 | ID=MsG0180005884.01.T01:exon:10757;Parent=MsG0180005884.01.T01 |
| Chr1 | CDS | 97118522 | 97118617 | 97118522 | ID=MsG0180005884.01.T01:cds;Parent=MsG0180005884.01.T01 |
| Chr1 | CDS | 97118964 | 97119411 | 97118964 | ID=MsG0180005884.01.T01:cds;Parent=MsG0180005884.01.T01 |
| Chr1 | CDS | 97119518 | 97119681 | 97119518 | ID=MsG0180005884.01.T01:cds;Parent=MsG0180005884.01.T01 |
| Chr1 | CDS | 97119803 | 97120061 | 97119803 | ID=MsG0180005884.01.T01:cds;Parent=MsG0180005884.01.T01 |
| Chr1 | CDS | 97120149 | 97120226 | 97120149 | ID=MsG0180005884.01.T01:cds;Parent=MsG0180005884.01.T01 |
| Chr1 | CDS | 97120510 | 97120664 | 97120510 | ID=MsG0180005884.01.T01:cds;Parent=MsG0180005884.01.T01 |
| Chr1 | CDS | 97120824 | 97121072 | 97120824 | ID=MsG0180005884.01.T01:cds;Parent=MsG0180005884.01.T01 |
| Gene Sequence |
| Protein sequence |
