AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180006238.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180006238.01.T01 MTR_1g116530 86.431 538 40 12 1 522 1 521 0.0 846
MsG0180006238.01.T01 MTR_6g086905 44.262 183 70 6 1 170 1 164 3.00e-33 124
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180006238.01.T01 AT1G54380 37.097 496 207 13 48 519 98 512 4.63e-82 265
MsG0180006238.01.T01 AT2G42510 32.364 275 79 8 82 314 300 509 8.36e-30 124
MsG0180006238.01.T01 AT2G42510 76.119 67 15 1 249 314 425 491 1.41e-25 110

Find 125 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCCAGAGGCCTGCGTTTC+AGG 0.095865 1:+101751438 MsG0180006238.01.T01:CDS
ACAATTTCGGGGAGATATTT+TGG 0.150062 1:+101752505 MsG0180006238.01.T01:CDS
AATATATTGGCTACAATTTC+GGG 0.157564 1:+101752493 MsG0180006238.01.T01:CDS
AAGTTACCTGGAAATAATTT+TGG 0.208567 1:+101752067 MsG0180006238.01.T01:CDS
AGCAGCAATCTTGTTGTTTC+CGG 0.209529 1:-101750027 None:intergenic
ATCTTGGAAGGCTTTCTTCT+TGG 0.213408 1:-101750197 None:intergenic
GAATATATTGGCTACAATTT+CGG 0.218549 1:+101752492 MsG0180006238.01.T01:CDS
TCTCTCAGCAGCACAAATTC+TGG 0.272998 1:+101750330 MsG0180006238.01.T01:CDS
GGAAGGCTTTCTTCTTGGTT+TGG 0.276604 1:-101750192 None:intergenic
TTTGAAAACCCTAAGGAGTT+AGG 0.280844 1:-101750128 None:intergenic
GGTTCAAGTGCCACGCATTC+TGG 0.282581 1:+101752019 MsG0180006238.01.T01:CDS
GAACGACAATTCATGGAAAT+TGG 0.288736 1:+101751289 MsG0180006238.01.T01:CDS
GGTGGACCAGAATCAAAATT+AGG 0.292136 1:-101751472 None:intergenic
GAAGGCTTTCTTCTTGGTTT+GGG 0.300427 1:-101750191 None:intergenic
AGTTTGGATACCTTCACTTT+AGG 0.303255 1:-101751733 None:intergenic
ATCACTCCAAAATTATTTCC+AGG 0.334285 1:-101752073 None:intergenic
TATATAACCATACATGTTCT+TGG 0.341499 1:-101752595 None:intergenic
TCTTTAGCAGTCAAGTTAGT+AGG 0.341512 1:-101752148 None:intergenic
ATTGTCATTAAGTTGCAAAC+TGG 0.345242 1:-101752548 None:intergenic
GTTCTTGTTGGTCTTCATCT+TGG 0.346146 1:-101750213 None:intergenic
AGATATTCCCATCCGTCTTC+TGG 0.348856 1:-101751493 None:intergenic
AAGTTTACTTCTATCAAGTT+TGG 0.350512 1:-101751749 None:intergenic
CTTGTCAGTGCTGCTACTTC+CGG 0.355328 1:-101750240 None:intergenic
TCAGGACACTTGGCAATATC+AGG 0.356178 1:-101751805 None:intergenic
TGCAGTGTATTGTGGAAATA+TGG 0.360062 1:+101751261 MsG0180006238.01.T01:CDS
GGATAATGAACGACAATTCA+TGG 0.363389 1:+101751282 MsG0180006238.01.T01:CDS
ACTTGGCAATATCAGGAATC+TGG 0.365174 1:-101751798 None:intergenic
GGTTGTAATGTTGAATATAT+TGG 0.370085 1:+101752480 MsG0180006238.01.T01:CDS
CCGTGCTGGTAAAGCTGAAC+TGG 0.379253 1:+101752450 MsG0180006238.01.T01:CDS
AAGACGGATGGGAATATCTT+AGG 0.380050 1:+101751497 MsG0180006238.01.T01:CDS
ACTTCCGGTGTTGTTCTTGT+TGG 0.398214 1:-101750225 None:intergenic
GGGAATATCTTAGGCGTGTC+AGG 0.399604 1:+101751506 MsG0180006238.01.T01:CDS
ACTCCCAACCCTAACTCCTT+AGG 0.401662 1:+101750119 MsG0180006238.01.T01:CDS
GTGAGAATTGGTTTCTTGTT+GGG 0.410292 1:-101750461 None:intergenic
CTACCTGAAACGCAGGCCTC+TGG 0.412069 1:-101751441 None:intergenic
ATATCCTCCCATTGTTTCAA+TGG 0.412617 1:-101751838 None:intergenic
TTGTTGGTCTTCATCTTGGA+AGG 0.417124 1:-101750209 None:intergenic
GAGTACTAACCAGTGAGAAT+TGG 0.421352 1:-101750473 None:intergenic
CTTATTTCCTCAAAGTTACC+TGG 0.429187 1:+101752055 MsG0180006238.01.T01:CDS
AATCTTGTTGTTTCCGGTGA+CGG 0.443780 1:-101750021 None:intergenic
CCCCTGCTTTCAACAATCCT+AGG 0.446314 1:+101752247 MsG0180006238.01.T01:CDS
TTGAAAACCCTAAGGAGTTA+GGG 0.448429 1:-101750127 None:intergenic
CTCCCAACCCTAACTCCTTA+GGG 0.451122 1:+101750120 MsG0180006238.01.T01:CDS
ATTATTTCCAGGTAACTTTG+AGG 0.459577 1:-101752062 None:intergenic
AACCCTAAGGAGTTAGGGTT+GGG 0.463119 1:-101750122 None:intergenic
AAAGAGTAATCATTCATTCA+TGG 0.463258 1:+101749887 MsG0180006238.01.T01:exon
TTCAATGGCAACAGATGCTC+AGG 0.464041 1:-101751823 None:intergenic
GAAGTACTCTCGCGAGATGA+TGG 0.464629 1:+101750286 MsG0180006238.01.T01:CDS
CTTGGCAATATCAGGAATCT+GGG 0.464802 1:-101751797 None:intergenic
TTGTCATTAAGTTGCAAACT+GGG 0.468866 1:-101752547 None:intergenic
AGTGAGAATTGGTTTCTTGT+TGG 0.480721 1:-101750462 None:intergenic
TCCCTAGGATTGTTGAAAGC+AGG 0.480836 1:-101752249 None:intergenic
GATGATGCCGCCACCGTCAC+CGG 0.481458 1:+101750008 MsG0180006238.01.T01:CDS
GTTGTTTCCGGTGACGGTGG+CGG 0.486317 1:-101750015 None:intergenic
TGAGGATAGCACCGTGCCTC+CGG 0.486698 1:+101752171 MsG0180006238.01.T01:CDS
ATAAGACTATGAGTATAAGA+AGG 0.488171 1:-101751334 None:intergenic
CAGAATCTGTCACGTATTGA+AGG 0.488568 1:+101751998 MsG0180006238.01.T01:intron
TTGTTCGTTACACTTTGAAA+CGG 0.492566 1:-101749919 None:intergenic
CATAGTCTTATGAGTAAGCA+TGG 0.498031 1:+101751346 MsG0180006238.01.T01:CDS
GTTAGGGTTGGGAGTAGCGA+TGG 0.498522 1:-101750111 None:intergenic
AGGCAAGAAGAAACGTCTCT+TGG 0.501980 1:+101750064 MsG0180006238.01.T01:CDS
CAACAGATGCTCAGGACACT+TGG 0.502613 1:-101751815 None:intergenic
CTTTCAGGTGCAGTGTATTG+TGG 0.521928 1:+101751253 MsG0180006238.01.T01:intron
ATCTGTTGCCATTGAAACAA+TGG 0.523105 1:+101751830 MsG0180006238.01.T01:CDS
TCTGTTGCCATTGAAACAAT+GGG 0.525124 1:+101751831 MsG0180006238.01.T01:CDS
TATGGAGCCAAGAACATGTA+TGG 0.527323 1:+101752588 MsG0180006238.01.T01:three_prime_UTR
TTGGTTGTTGTGAGCAGCGG+CGG 0.530967 1:-101750414 None:intergenic
CTTCATAATAACACGAGCAT+TGG 0.532195 1:+101752112 MsG0180006238.01.T01:CDS
GATTGTTGAAAGCAGGGGAG+TGG 0.532703 1:-101752242 None:intergenic
CACACTTCCGCAGCAGACTC+CGG 0.533825 1:-101752420 None:intergenic
CCCTAGGATTGTTGAAAGCA+GGG 0.537138 1:-101752248 None:intergenic
TTTCAGCAACATTGAGACTC+GGG 0.544817 1:-101752284 None:intergenic
CTCAATGTTTGAAAACCCTA+AGG 0.548189 1:-101750135 None:intergenic
AAACCCTAAGGAGTTAGGGT+TGG 0.550292 1:-101750123 None:intergenic
CGACAATTCATGGAAATTGG+TGG 0.550323 1:+101751292 MsG0180006238.01.T01:CDS
TTGTTGGGAAGGAACGGAAG+AGG 0.550366 1:-101750446 None:intergenic
GAATTGGTTTCTTGTTGGGA+AGG 0.550414 1:-101750457 None:intergenic
CTTGTTGTTTCCGGTGACGG+TGG 0.556420 1:-101750018 None:intergenic
CTGGTCCACCAGAAGACGGA+TGG 0.561101 1:+101751485 MsG0180006238.01.T01:CDS
TAATAACACGAGCATTGGTA+AGG 0.562700 1:+101752117 MsG0180006238.01.T01:CDS
TGCTGATGAGTATGATACAT+TGG 0.568733 1:+101750385 MsG0180006238.01.T01:CDS
CCCTGCTTTCAACAATCCTA+GGG 0.569746 1:+101752248 MsG0180006238.01.T01:CDS
GCACAAGTGTCTGCATCTAG+TGG 0.569910 1:-101752391 None:intergenic
CTGCACTCCGGAGTCTGCTG+CGG 0.570655 1:+101752413 MsG0180006238.01.T01:CDS
AGGGTTGGGAGTAGCGATGG+TGG 0.572338 1:-101750108 None:intergenic
AACAAGAAACCAATTCTCAC+TGG 0.572694 1:+101750464 MsG0180006238.01.T01:CDS
TATTCCCATCCGTCTTCTGG+TGG 0.573091 1:-101751490 None:intergenic
TACAGGATTCTCCGGAGGCA+CGG 0.575594 1:-101752182 None:intergenic
GTTTCAGCAACATTGAGACT+CGG 0.575650 1:-101752285 None:intergenic
GATGCATAATGTCTAATGTA+TGG 0.576560 1:+101752627 MsG0180006238.01.T01:three_prime_UTR
GGTTTCTTGTTGGGAAGGAA+CGG 0.577664 1:-101750452 None:intergenic
GATTCTGGTCCACCAGAAGA+CGG 0.580030 1:+101751481 MsG0180006238.01.T01:CDS
GCGAGATGATGGAGTCTATG+AGG 0.581842 1:+101750297 MsG0180006238.01.T01:CDS
TTGTAGATTGCCAGAATGCG+TGG 0.584277 1:-101752029 None:intergenic
AAGTGTGCTAGCATCCGTGC+TGG 0.589286 1:+101752436 MsG0180006238.01.T01:CDS
AGGCCTGCGTTTCAGGTAGA+CGG 0.590675 1:+101751445 MsG0180006238.01.T01:CDS
TCTTTACTTGCCTAGGTGGG+AGG 0.592867 1:+101751699 MsG0180006238.01.T01:intron
CCAGTTCAGCTTTACCAGCA+CGG 0.594914 1:-101752450 None:intergenic
GCTTTCAACAATCCTAGGGA+TGG 0.595935 1:+101752252 MsG0180006238.01.T01:CDS
TCTCCGTCTACCTGAAACGC+AGG 0.596684 1:-101751448 None:intergenic
CAGACACTTGTGCTGCACTC+CGG 0.597128 1:+101752401 MsG0180006238.01.T01:CDS
TCCTGTATTGAAAACATCCG+TGG 0.601385 1:+101752198 MsG0180006238.01.T01:CDS
CATGCAATCAATTCTTGTGA+TGG 0.606582 1:-101752335 None:intergenic
TCACAAGAATTGATTGCATG+TGG 0.606890 1:+101752338 MsG0180006238.01.T01:CDS
TAAGACTATGAGTATAAGAA+GGG 0.611576 1:-101751333 None:intergenic
ACATCAAATTCCTAAAGTGA+AGG 0.612737 1:+101751723 MsG0180006238.01.T01:CDS
GAGTATAAGAAGGGTCAACA+TGG 0.624659 1:-101751324 None:intergenic
TGGTCCACCAGAAGACGGAT+GGG 0.631512 1:+101751486 MsG0180006238.01.T01:CDS
TAACTTGACTGCTAAAGATG+AGG 0.640802 1:+101752153 MsG0180006238.01.T01:CDS
ATAGTCTTATGAGTAAGCAT+GGG 0.641197 1:+101751347 MsG0180006238.01.T01:CDS
TGATAGAAGTAAACTTAACA+AGG 0.642496 1:+101751756 MsG0180006238.01.T01:CDS
CCTAGGATTGTTGAAAGCAG+GGG 0.642812 1:-101752247 None:intergenic
GGGCTACAGAGTCCATCCCT+AGG 0.642846 1:-101752264 None:intergenic
AAGACCAACAAGAACAACAC+CGG 0.646833 1:+101750221 MsG0180006238.01.T01:CDS
ATGATTATGCTAGCATCCAG+AGG 0.651012 1:+101751425 MsG0180006238.01.T01:CDS
TTCAATACAGGATTCTCCGG+AGG 0.653479 1:-101752187 None:intergenic
TAAAGCTGAACTGGATGATG+AGG 0.657096 1:+101752459 MsG0180006238.01.T01:CDS
GCTGCTCAACAACAACTCGA+AGG 0.657239 1:+101750044 MsG0180006238.01.T01:CDS
GCTGCTACTAGTTTGATCCA+CGG 0.659357 1:-101752215 None:intergenic
ACGGAAGAGGAGTGTGACGT+TGG 0.659680 1:-101750433 None:intergenic
ATATATTGGCTACAATTTCG+GGG 0.666684 1:+101752494 MsG0180006238.01.T01:CDS
ACGTTGGTTGTTGTGAGCAG+CGG 0.666754 1:-101750417 None:intergenic
TTGGCAATATCAGGAATCTG+GGG 0.686299 1:-101751796 None:intergenic
AGCAAAAGCAGATTGAAGCG+CGG 0.711511 1:-101750366 None:intergenic
GTTGCCATTGAAACAATGGG+AGG 0.739891 1:+101751834 MsG0180006238.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAGAAAGAAAAAAAAT+TGG - Chr1:101750890-101750909 None:intergenic 10.0%
!! TTAATAATACAAACACAATA+TGG - Chr1:101750592-101750611 None:intergenic 15.0%
!! TTGTTTAATAGTATATACTA+AGG + Chr1:101751945-101751964 MsG0180006238.01.T01:intron 15.0%
!! ATGAAAACTCAATAATAATC+AGG - Chr1:101751131-101751150 None:intergenic 20.0%
!! TTAAAGCATAAATTGATCAA+AGG - Chr1:101751565-101751584 None:intergenic 20.0%
!!! GACAATTTATGTTTTAGAAT+TGG + Chr1:101752565-101752584 MsG0180006238.01.T01:three_prime_UTR 20.0%
!!! GCAATTAAGTTTTACATATT+TGG - Chr1:101750668-101750687 None:intergenic 20.0%
!!! TTTATGTTTTAGAATTGGTA+TGG + Chr1:101752570-101752589 MsG0180006238.01.T01:three_prime_UTR 20.0%
!!! TTTCTTTCTTTTTTGTACTT+AGG + Chr1:101750896-101750915 MsG0180006238.01.T01:intron 20.0%
! AAAGAGTAATCATTCATTCA+TGG + Chr1:101749887-101749906 MsG0180006238.01.T01:exon 25.0%
! AAGTTTACTTCTATCAAGTT+TGG - Chr1:101751752-101751771 None:intergenic 25.0%
! AATAACAAAATTGGGAAACA+AGG - Chr1:101751977-101751996 None:intergenic 25.0%
! ATAACAAAATTGGGAAACAA+GGG - Chr1:101751976-101751995 None:intergenic 25.0%
! ATAAGACTATGAGTATAAGA+AGG - Chr1:101751337-101751356 None:intergenic 25.0%
! ATTCATTCTTGAAAGTTCAA+TGG - Chr1:101750815-101750834 None:intergenic 25.0%
! GGTTGTAATGTTGAATATAT+TGG + Chr1:101752480-101752499 MsG0180006238.01.T01:CDS 25.0%
! TAAGACTATGAGTATAAGAA+GGG - Chr1:101751336-101751355 None:intergenic 25.0%
! TATATAACCATACATGTTCT+TGG - Chr1:101752598-101752617 None:intergenic 25.0%
! TCTTCATGATAACTTTATCT+AGG + Chr1:101751595-101751614 MsG0180006238.01.T01:intron 25.0%
! TGATAGAAGTAAACTTAACA+AGG + Chr1:101751756-101751775 MsG0180006238.01.T01:CDS 25.0%
!! AAGTTACCTGGAAATAATTT+TGG + Chr1:101752067-101752086 MsG0180006238.01.T01:CDS 25.0%
!! AATATATTGGCTACAATTTC+GGG + Chr1:101752493-101752512 MsG0180006238.01.T01:CDS 25.0%
!! GAATATATTGGCTACAATTT+CGG + Chr1:101752492-101752511 MsG0180006238.01.T01:CDS 25.0%
!! GTAAATTTGAAACACTACTT+AGG + Chr1:101750991-101751010 MsG0180006238.01.T01:intron 25.0%
!! GTCAATTTGATGTTTTCAAT+TGG + Chr1:101750739-101750758 MsG0180006238.01.T01:intron 25.0%
!!! AGATTCTGGAATAACAAAAT+TGG - Chr1:101751986-101752005 None:intergenic 25.0%
!!! GATTCTGGAATAACAAAATT+GGG - Chr1:101751985-101752004 None:intergenic 25.0%
ACATCAAATTCCTAAAGTGA+AGG + Chr1:101751723-101751742 MsG0180006238.01.T01:CDS 30.0%
AGACTTTCAGCTTATGAAAA+TGG - Chr1:101751225-101751244 None:intergenic 30.0%
ATCACTCCAAAATTATTTCC+AGG - Chr1:101752076-101752095 None:intergenic 30.0%
ATTATTTCCAGGTAACTTTG+AGG - Chr1:101752065-101752084 None:intergenic 30.0%
GACTTTCAGCTTATGAAAAT+GGG - Chr1:101751224-101751243 None:intergenic 30.0%
GATGCATAATGTCTAATGTA+TGG + Chr1:101752627-101752646 MsG0180006238.01.T01:three_prime_UTR 30.0%
TTCAAGAATGAATCAGCAAA+GGG + Chr1:101750822-101750841 MsG0180006238.01.T01:intron 30.0%
TTTCAAGAATGAATCAGCAA+AGG + Chr1:101750821-101750840 MsG0180006238.01.T01:intron 30.0%
! AGAATTTTCAGTACTAAGAG+AGG + Chr1:101751870-101751889 MsG0180006238.01.T01:CDS 30.0%
! ATATATTGGCTACAATTTCG+GGG + Chr1:101752494-101752513 MsG0180006238.01.T01:CDS 30.0%
! ATTGTCATTAAGTTGCAAAC+TGG - Chr1:101752551-101752570 None:intergenic 30.0%
! GATAACTTTATCTAGGATTC+TGG + Chr1:101751602-101751621 MsG0180006238.01.T01:intron 30.0%
! TTGTCATTAAGTTGCAAACT+GGG - Chr1:101752550-101752569 None:intergenic 30.0%
! TTGTTCGTTACACTTTGAAA+CGG - Chr1:101749922-101749941 None:intergenic 30.0%
! TTTGATGTTTTCAATTGGAC+AGG + Chr1:101750744-101750763 MsG0180006238.01.T01:intron 30.0%
!! AAAGTCTTGTTATTGCTTTC+AGG + Chr1:101751238-101751257 MsG0180006238.01.T01:intron 30.0%
!! AACGTGTTATTTCTACTACT+AGG - Chr1:101751671-101751690 None:intergenic 30.0%
!! ATAGTCTTATGAGTAAGCAT+GGG + Chr1:101751347-101751366 MsG0180006238.01.T01:CDS 30.0%
!! TGTTATTTCTACTACTAGGA+AGG - Chr1:101751667-101751686 None:intergenic 30.0%
AAACAACAAAGCAATCCTCA+TGG - Chr1:101751031-101751050 None:intergenic 35.0%
AACAACAAAGCAATCCTCAT+GGG - Chr1:101751030-101751049 None:intergenic 35.0%
AACAAGAAACCAATTCTCAC+TGG + Chr1:101750464-101750483 MsG0180006238.01.T01:CDS 35.0%
AGTTTGGATACCTTCACTTT+AGG - Chr1:101751736-101751755 None:intergenic 35.0%
ATATCCTCCCATTGTTTCAA+TGG - Chr1:101751841-101751860 None:intergenic 35.0%
ATCTGTTGCCATTGAAACAA+TGG + Chr1:101751830-101751849 MsG0180006238.01.T01:CDS 35.0%
CAAGATTCACGATTCTAGAT+AGG - Chr1:101750621-101750640 None:intergenic 35.0%
CATGCAATCAATTCTTGTGA+TGG - Chr1:101752338-101752357 None:intergenic 35.0%
CTCAATGTTTGAAAACCCTA+AGG - Chr1:101750138-101750157 None:intergenic 35.0%
CTTAAACTCTTTACTTGCCT+AGG + Chr1:101751692-101751711 MsG0180006238.01.T01:intron 35.0%
CTTATTTCCTCAAAGTTACC+TGG + Chr1:101752055-101752074 MsG0180006238.01.T01:CDS 35.0%
CTTCATAATAACACGAGCAT+TGG + Chr1:101752112-101752131 MsG0180006238.01.T01:CDS 35.0%
GAACGACAATTCATGGAAAT+TGG + Chr1:101751289-101751308 MsG0180006238.01.T01:CDS 35.0%
GGATAATGAACGACAATTCA+TGG + Chr1:101751282-101751301 MsG0180006238.01.T01:CDS 35.0%
TAACTTGACTGCTAAAGATG+AGG + Chr1:101752153-101752172 MsG0180006238.01.T01:CDS 35.0%
TCACAAGAATTGATTGCATG+TGG + Chr1:101752338-101752357 MsG0180006238.01.T01:CDS 35.0%
TCTGTTGCCATTGAAACAAT+GGG + Chr1:101751831-101751850 MsG0180006238.01.T01:CDS 35.0%
TCTTTAGCAGTCAAGTTAGT+AGG - Chr1:101752151-101752170 None:intergenic 35.0%
TGCAGTGTATTGTGGAAATA+TGG + Chr1:101751261-101751280 MsG0180006238.01.T01:CDS 35.0%
TGCTGATGAGTATGATACAT+TGG + Chr1:101750385-101750404 MsG0180006238.01.T01:CDS 35.0%
TTGAAAACCCTAAGGAGTTA+GGG - Chr1:101750130-101750149 None:intergenic 35.0%
TTTGAAAACCCTAAGGAGTT+AGG - Chr1:101750131-101750150 None:intergenic 35.0%
! ACAATTTCGGGGAGATATTT+TGG + Chr1:101752505-101752524 MsG0180006238.01.T01:CDS 35.0%
! AGAACTCATCTCTGTCTTTT+GGG + Chr1:101751904-101751923 MsG0180006238.01.T01:intron 35.0%
! AGTGAGAATTGGTTTCTTGT+TGG - Chr1:101750465-101750484 None:intergenic 35.0%
! CTTAGGGTTTTCAAACATTG+AGG + Chr1:101750136-101750155 MsG0180006238.01.T01:CDS 35.0%
! GTGAGAATTGGTTTCTTGTT+GGG - Chr1:101750464-101750483 None:intergenic 35.0%
! GTTTTCAATACAGGATTCTC+CGG - Chr1:101752193-101752212 None:intergenic 35.0%
! TAGAACTCATCTCTGTCTTT+TGG + Chr1:101751903-101751922 MsG0180006238.01.T01:intron 35.0%
! TGATGATGATGATGATAGTG+AGG + Chr1:101751402-101751421 MsG0180006238.01.T01:CDS 35.0%
! TTGCAGTTAGATCTTTTTCC+AGG - Chr1:101750850-101750869 None:intergenic 35.0%
!! ATTCTTGTGATGGTGTTTTC+AGG - Chr1:101752328-101752347 None:intergenic 35.0%
!! CATAGTCTTATGAGTAAGCA+TGG + Chr1:101751346-101751365 MsG0180006238.01.T01:CDS 35.0%
!! TAATAACACGAGCATTGGTA+AGG + Chr1:101752117-101752136 MsG0180006238.01.T01:CDS 35.0%
!! TTATGAAAATGGGTTGAAGC+TGG - Chr1:101751214-101751233 None:intergenic 35.0%
!!! GGAGAACCTAATTTTGATTC+TGG + Chr1:101751466-101751485 MsG0180006238.01.T01:CDS 35.0%
AAACTCTTTACTTGCCTAGG+TGG + Chr1:101751695-101751714 MsG0180006238.01.T01:intron 40.0%
AACTCTTTACTTGCCTAGGT+GGG + Chr1:101751696-101751715 MsG0180006238.01.T01:intron 40.0%
AAGACCAACAAGAACAACAC+CGG + Chr1:101750221-101750240 MsG0180006238.01.T01:CDS 40.0%
AAGACGGATGGGAATATCTT+AGG + Chr1:101751497-101751516 MsG0180006238.01.T01:CDS 40.0%
ACAAAGAAGCCTACAACACA+TGG - Chr1:101750973-101750992 None:intergenic 40.0%
ACTTGGCAATATCAGGAATC+TGG - Chr1:101751801-101751820 None:intergenic 40.0%
ATCTTGGAAGGCTTTCTTCT+TGG - Chr1:101750200-101750219 None:intergenic 40.0%
ATGATTATGCTAGCATCCAG+AGG + Chr1:101751425-101751444 MsG0180006238.01.T01:CDS 40.0%
CACAATATGAGCTAACAAGC+AGG - Chr1:101750720-101750739 None:intergenic 40.0%
CAGAATCTGTCACGTATTGA+AGG + Chr1:101751998-101752017 MsG0180006238.01.T01:intron 40.0%
CATGAAAAGCCATGTGTTGT+AGG + Chr1:101750961-101750980 MsG0180006238.01.T01:intron 40.0%
CGACAATTCATGGAAATTGG+TGG + Chr1:101751292-101751311 MsG0180006238.01.T01:CDS 40.0%
CTCAGAAACACACACACATA+TGG - Chr1:101750562-101750581 None:intergenic 40.0%
CTTCAATACGTGACAGATTC+TGG - Chr1:101752000-101752019 None:intergenic 40.0%
CTTGGCAATATCAGGAATCT+GGG - Chr1:101751800-101751819 None:intergenic 40.0%
GAAGGCTTTCTTCTTGGTTT+GGG - Chr1:101750194-101750213 None:intergenic 40.0%
GACATATCTGCATCTTCCAT+TGG - Chr1:101750781-101750800 None:intergenic 40.0%
GAGTACTAACCAGTGAGAAT+TGG - Chr1:101750476-101750495 None:intergenic 40.0%
GAGTATAAGAAGGGTCAACA+TGG - Chr1:101751327-101751346 None:intergenic 40.0%
GGTGGACCAGAATCAAAATT+AGG - Chr1:101751475-101751494 None:intergenic 40.0%
GTTTCAGCAACATTGAGACT+CGG - Chr1:101752288-101752307 None:intergenic 40.0%
TAAAGCTGAACTGGATGATG+AGG + Chr1:101752459-101752478 MsG0180006238.01.T01:CDS 40.0%
TATGGAGCCAAGAACATGTA+TGG + Chr1:101752588-101752607 MsG0180006238.01.T01:three_prime_UTR 40.0%
TCCTGTATTGAAAACATCCG+TGG + Chr1:101752198-101752217 MsG0180006238.01.T01:CDS 40.0%
TTGATCAAAGGACCAGCAAT+TGG - Chr1:101751553-101751572 None:intergenic 40.0%
TTGGCAATATCAGGAATCTG+GGG - Chr1:101751799-101751818 None:intergenic 40.0%
TTTCAGCAACATTGAGACTC+GGG - Chr1:101752287-101752306 None:intergenic 40.0%
! GAATTGGTTTCTTGTTGGGA+AGG - Chr1:101750460-101750479 None:intergenic 40.0%
! GTTTTCAATTGGACAGGTGT+GGG + Chr1:101750750-101750769 MsG0180006238.01.T01:intron 40.0%
! TCCACGGATGTTTTCAATAC+AGG - Chr1:101752202-101752221 None:intergenic 40.0%
! TGTTTTCAATTGGACAGGTG+TGG + Chr1:101750749-101750768 MsG0180006238.01.T01:intron 40.0%
!! AATCTTGTTGTTTCCGGTGA+CGG - Chr1:101750024-101750043 None:intergenic 40.0%
!! AGCAGCAATCTTGTTGTTTC+CGG - Chr1:101750030-101750049 None:intergenic 40.0%
!! GTTCTTGTTGGTCTTCATCT+TGG - Chr1:101750216-101750235 None:intergenic 40.0%
!! TTGTTGGTCTTCATCTTGGA+AGG - Chr1:101750212-101750231 None:intergenic 40.0%
AAACCCTAAGGAGTTAGGGT+TGG - Chr1:101750126-101750145 None:intergenic 45.0%
AACCCTAAGGAGTTAGGGTT+GGG - Chr1:101750125-101750144 None:intergenic 45.0%
AGATATTCCCATCCGTCTTC+TGG - Chr1:101751496-101751515 None:intergenic 45.0%
AGCAAAAGCAGATTGAAGCG+CGG - Chr1:101750369-101750388 None:intergenic 45.0%
AGGCAAGAAGAAACGTCTCT+TGG + Chr1:101750064-101750083 MsG0180006238.01.T01:CDS 45.0%
ATGAATCAGCAAAGGGAACC+TGG + Chr1:101750829-101750848 MsG0180006238.01.T01:intron 45.0%
CCCTAGGATTGTTGAAAGCA+GGG - Chr1:101752251-101752270 None:intergenic 45.0%
CCCTGCTTTCAACAATCCTA+GGG + Chr1:101752248-101752267 MsG0180006238.01.T01:CDS 45.0%
CCTAGGATTGTTGAAAGCAG+GGG - Chr1:101752250-101752269 None:intergenic 45.0%
CTAACAAGCAGGAGACTGTT+GGG - Chr1:101750709-101750728 None:intergenic 45.0%
GCTTTCAACAATCCTAGGGA+TGG + Chr1:101752252-101752271 MsG0180006238.01.T01:CDS 45.0%
GGAAGGCTTTCTTCTTGGTT+TGG - Chr1:101750195-101750214 None:intergenic 45.0%
GGTTTCTTGTTGGGAAGGAA+CGG - Chr1:101750455-101750474 None:intergenic 45.0%
GTTGCCATTGAAACAATGGG+AGG + Chr1:101751834-101751853 MsG0180006238.01.T01:CDS 45.0%
TCAGGACACTTGGCAATATC+AGG - Chr1:101751808-101751827 None:intergenic 45.0%
TCCCTAGGATTGTTGAAAGC+AGG - Chr1:101752252-101752271 None:intergenic 45.0%
TCTCTCAGCAGCACAAATTC+TGG + Chr1:101750330-101750349 MsG0180006238.01.T01:CDS 45.0%
TTCAATACAGGATTCTCCGG+AGG - Chr1:101752190-101752209 None:intergenic 45.0%
TTCAATGGCAACAGATGCTC+AGG - Chr1:101751826-101751845 None:intergenic 45.0%
TTGTAGATTGCCAGAATGCG+TGG - Chr1:101752032-101752051 None:intergenic 45.0%
! CTTTCAGGTGCAGTGTATTG+TGG + Chr1:101751253-101751272 MsG0180006238.01.T01:intron 45.0%
! GCTGCTACTAGTTTGATCCA+CGG - Chr1:101752218-101752237 None:intergenic 45.0%
!! ACTTCCGGTGTTGTTCTTGT+TGG - Chr1:101750228-101750247 None:intergenic 45.0%
AACTGTCAGCTGCCAATTGC+TGG + Chr1:101751538-101751557 MsG0180006238.01.T01:intron 50.0%
ACTCCCAACCCTAACTCCTT+AGG + Chr1:101750119-101750138 MsG0180006238.01.T01:CDS 50.0%
ATTTGATGTGCCTCCCACCT+AGG - Chr1:101751712-101751731 None:intergenic 50.0%
CAACAGATGCTCAGGACACT+TGG - Chr1:101751818-101751837 None:intergenic 50.0%
CCAGTTCAGCTTTACCAGCA+CGG - Chr1:101752453-101752472 None:intergenic 50.0%
CCCCTGCTTTCAACAATCCT+AGG + Chr1:101752247-101752266 MsG0180006238.01.T01:CDS 50.0%
CTCCCAACCCTAACTCCTTA+GGG + Chr1:101750120-101750139 MsG0180006238.01.T01:CDS 50.0%
GATTCTGGTCCACCAGAAGA+CGG + Chr1:101751481-101751500 MsG0180006238.01.T01:CDS 50.0%
GATTGTTGAAAGCAGGGGAG+TGG - Chr1:101752245-101752264 None:intergenic 50.0%
GCACAAGTGTCTGCATCTAG+TGG - Chr1:101752394-101752413 None:intergenic 50.0%
GCTAACAAGCAGGAGACTGT+TGG - Chr1:101750710-101750729 None:intergenic 50.0%
GCTGCTCAACAACAACTCGA+AGG + Chr1:101750044-101750063 MsG0180006238.01.T01:CDS 50.0%
GGGAATATCTTAGGCGTGTC+AGG + Chr1:101751506-101751525 MsG0180006238.01.T01:CDS 50.0%
GTCATACAGTAGCACCCATG+AGG + Chr1:101751013-101751032 MsG0180006238.01.T01:intron 50.0%
TATTCCCATCCGTCTTCTGG+TGG - Chr1:101751493-101751512 None:intergenic 50.0%
TCTTTACTTGCCTAGGTGGG+AGG + Chr1:101751699-101751718 MsG0180006238.01.T01:intron 50.0%
TTGTTGGGAAGGAACGGAAG+AGG - Chr1:101750449-101750468 None:intergenic 50.0%
! ACGTTGGTTGTTGTGAGCAG+CGG - Chr1:101750420-101750439 None:intergenic 50.0%
! CTTGTCAGTGCTGCTACTTC+CGG - Chr1:101750243-101750262 None:intergenic 50.0%
! GAAGTACTCTCGCGAGATGA+TGG + Chr1:101750286-101750305 MsG0180006238.01.T01:CDS 50.0%
!! GCGAGATGATGGAGTCTATG+AGG + Chr1:101750297-101750316 MsG0180006238.01.T01:CDS 50.0%
AAGTGTGCTAGCATCCGTGC+TGG + Chr1:101752436-101752455 MsG0180006238.01.T01:CDS 55.0%
CAGACACTTGTGCTGCACTC+CGG + Chr1:101752401-101752420 MsG0180006238.01.T01:CDS 55.0%
GGTTCAAGTGCCACGCATTC+TGG + Chr1:101752019-101752038 MsG0180006238.01.T01:CDS 55.0%
GTTAGGGTTGGGAGTAGCGA+TGG - Chr1:101750114-101750133 None:intergenic 55.0%
TACAGGATTCTCCGGAGGCA+CGG - Chr1:101752185-101752204 None:intergenic 55.0%
TCTCCGTCTACCTGAAACGC+AGG - Chr1:101751451-101751470 None:intergenic 55.0%
TGGTCCACCAGAAGACGGAT+GGG + Chr1:101751486-101751505 MsG0180006238.01.T01:CDS 55.0%
TTGGTTGTTGTGAGCAGCGG+CGG - Chr1:101750417-101750436 None:intergenic 55.0%
! ACGGAAGAGGAGTGTGACGT+TGG - Chr1:101750436-101750455 None:intergenic 55.0%
! AGGCCTGCGTTTCAGGTAGA+CGG + Chr1:101751445-101751464 MsG0180006238.01.T01:CDS 55.0%
! CCGTGCTGGTAAAGCTGAAC+TGG + Chr1:101752450-101752469 MsG0180006238.01.T01:CDS 55.0%
!! CTTGTTGTTTCCGGTGACGG+TGG - Chr1:101750021-101750040 None:intergenic 55.0%
AGGGTTGGGAGTAGCGATGG+TGG - Chr1:101750111-101750130 None:intergenic 60.0%
CACACTTCCGCAGCAGACTC+CGG - Chr1:101752423-101752442 None:intergenic 60.0%
CATCCAGAGGCCTGCGTTTC+AGG + Chr1:101751438-101751457 MsG0180006238.01.T01:CDS 60.0%
CTACCTGAAACGCAGGCCTC+TGG - Chr1:101751444-101751463 None:intergenic 60.0%
CTGGTCCACCAGAAGACGGA+TGG + Chr1:101751485-101751504 MsG0180006238.01.T01:CDS 60.0%
GGGCTACAGAGTCCATCCCT+AGG - Chr1:101752267-101752286 None:intergenic 60.0%
TGAGGATAGCACCGTGCCTC+CGG + Chr1:101752171-101752190 MsG0180006238.01.T01:CDS 60.0%
! ACAGGTGTGGGTGCTGCCAA+TGG + Chr1:101750762-101750781 MsG0180006238.01.T01:intron 60.0%
! GTTGTTTCCGGTGACGGTGG+CGG - Chr1:101750018-101750037 None:intergenic 60.0%
CTGCACTCCGGAGTCTGCTG+CGG + Chr1:101752413-101752432 MsG0180006238.01.T01:CDS 65.0%
GATGATGCCGCCACCGTCAC+CGG + Chr1:101750008-101750027 MsG0180006238.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 101749841 101752696 101749841 ID=MsG0180006238.01;Name=MsG0180006238.01
Chr1 mRNA 101749841 101752696 101749841 ID=MsG0180006238.01.T01;Parent=MsG0180006238.01;Name=MsG0180006238.01.T01;_AED=0.45;_eAED=0.45;_QI=65|0.66|0.75|1|1|1|4|153|522
Chr1 exon 101749841 101750485 101749841 ID=MsG0180006238.01.T01:exon:15227;Parent=MsG0180006238.01.T01
Chr1 exon 101751260 101751527 101751260 ID=MsG0180006238.01.T01:exon:15228;Parent=MsG0180006238.01.T01
Chr1 exon 101751714 101751891 101751714 ID=MsG0180006238.01.T01:exon:15229;Parent=MsG0180006238.01.T01
Chr1 exon 101752001 101752696 101752001 ID=MsG0180006238.01.T01:exon:15230;Parent=MsG0180006238.01.T01
Chr1 five_prime_UTR 101749841 101749905 101749841 ID=MsG0180006238.01.T01:five_prime_utr;Parent=MsG0180006238.01.T01
Chr1 CDS 101749906 101750485 101749906 ID=MsG0180006238.01.T01:cds;Parent=MsG0180006238.01.T01
Chr1 CDS 101751260 101751527 101751260 ID=MsG0180006238.01.T01:cds;Parent=MsG0180006238.01.T01
Chr1 CDS 101751714 101751891 101751714 ID=MsG0180006238.01.T01:cds;Parent=MsG0180006238.01.T01
Chr1 CDS 101752001 101752543 101752001 ID=MsG0180006238.01.T01:cds;Parent=MsG0180006238.01.T01
Chr1 three_prime_UTR 101752544 101752696 101752544 ID=MsG0180006238.01.T01:three_prime_utr;Parent=MsG0180006238.01.T01
Gene Sequence

>MsG0180006238.01.T01

ATGGCTGAATTAACCGTTTCAAAGTGTAACGAACAACAACAAGAAGAAGAAGAAGAAGAAGAACTCGTCGTCAACAAGATTCAAATTGATGATGATGATGATGATGATGCCGCCACCGTCACCGGAAACAACAAGATTGCTGCTCAACAACAACTCGAAGGCAAGAAGAAACGTCTCTTGGAAGAACTCGAATCTGTTTTCACCACCATCGCTACTCCCAACCCTAACTCCTTAGGGTTTTCAAACATTGAGGTCATTGATGAAACTGCTTTGCTCGATTGCATTCCCAAACCAAGAAGAAAGCCTTCCAAGATGAAGACCAACAAGAACAACACCGGAAGTAGCAGCACTGACAAGATGATGATGATGAAGAAGAAGAAGAAGTACTCTCGCGAGATGATGGAGTCTATGAGGTTTGTGAATGTCTCTCAGCAGCACAAATTCTGGAAAACAATCTACACCGCGCTTCAATCTGCTTTTGCTGATGAGTATGATACATTGGTCGTTGCCGCCGCTGCTCACAACAACCAACGTCACACTCCTCTTCCGTTCCTTCCCAACAAGAAACCAATTCTCACTGGTGCAGTGTATTGTGGAAATATGGATAATGAACGACAATTCATGGAAATTGGTGGAAGTGTGAGCCATGTTGACCCTTCTTATACTCATAGTCTTATGAGTAAGCATGGGTGCTCAGTTTCAGAAGAGTGCATTGAAAATGATGATGATGATGATGATAGTGAGGATGATTATGCTAGCATCCAGAGGCCTGCGTTTCAGGTAGACGGAGAACCTAATTTTGATTCTGGTCCACCAGAAGACGGATGGGAATATCTTAGGCGTGTCAGGTGGGAGGCACATCAAATTCCTAAAGTGAAGGTATCCAAACTTGATAGAAGTAAACTTAACAAGGAGCAAAGTGCTTATATGCCCCAGATTCCTGATATTGCCAAGTGTCCTGAGCATCTGTTGCCATTGAAACAATGGGAGGATATATTTCTTGCAGAATTTTCAGTACTAAGAGAGAATCTGTCACGTATTGAAGGTTCAAGTGCCACGCATTCTGGCAATCTACAATCTCTTATTTCCTCAAAGTTACCTGGAAATAATTTTGGAGTGATGAACAGAGATGTGCTACTTCATAATAACACGAGCATTGGTAAGGCTGATCAACCTACTAACTTGACTGCTAAAGATGAGGATAGCACCGTGCCTCCGGAGAATCCTGTATTGAAAACATCCGTGGATCAAACTAGTAGCAGCTGTTCCACTCCCCTGCTTTCAACAATCCTAGGGATGGACTCTGTAGCCCGAGTCTCAATGTTGCTGAAACGAATTCGTTTGCTAGAGCCTGAAAACACCATCACAAGAATTGATTGCATGTGGCTTTTTGCTCTCTGTGCAGCAGTAGATGCTCCACTAGATGCAGACACTTGTGCTGCACTCCGGAGTCTGCTGCGGAAGTGTGCTAGCATCCGTGCTGGTAAAGCTGAACTGGATGATGAGGTTGTAATGTTGAATATATTGGCTACAATTTCGGGGAGATATTTTGGACAATCTGAAAATTGA
Protein sequence

>MsG0180006238.01.T01

MAELTVSKCNEQQQEEEEEEELVVNKIQIDDDDDDDAATVTGNNKIAAQQQLEGKKKRLLEELESVFTTIATPNPNSLGFSNIEVIDETALLDCIPKPRRKPSKMKTNKNNTGSSSTDKMMMMKKKKKYSREMMESMRFVNVSQQHKFWKTIYTALQSAFADEYDTLVVAAAAHNNQRHTPLPFLPNKKPILTGAVYCGNMDNERQFMEIGGSVSHVDPSYTHSLMSKHGCSVSEECIENDDDDDDSEDDYASIQRPAFQVDGEPNFDSGPPEDGWEYLRRVRWEAHQIPKVKVSKLDRSKLNKEQSAYMPQIPDIAKCPEHLLPLKQWEDIFLAEFSVLRENLSRIEGSSATHSGNLQSLISSKLPGNNFGVMNRDVLLHNNTSIGKADQPTNLTAKDEDSTVPPENPVLKTSVDQTSSSCSTPLLSTILGMDSVARVSMLLKRIRLLEPENTITRIDCMWLFALCAAVDAPLDADTCAALRSLLRKCASIRAGKAELDDEVVMLNILATISGRYFGQSEN*