AlfalfaGEDB | MsG0280006291.01

M. sativa cultivar ZhongmuNo.1 / MsG0280006291.01

KEGG: -
GO: GO:0005215 GO:0006857 GO:0016020 GO:0022857 GO:0055085
PFAM: PF00854

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006291.01.T01	XP_003592869.1	98.649	592	7	1	1	591	1	592	0	1197

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006291.01.T01	Q93VV5	71.927	545	139	4	28	558	29	573	0	815

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006291.01.T01	Q1SKS7	98.649	592	7	1	1	591	1	592	0.0	1197

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0180001738.01	MsG0280006291.01	PPI
MsG0180001746.01	MsG0280006291.01	PPI
MsG0180001755.01	MsG0280006291.01	PPI
MsG0180003268.01	MsG0280006291.01	PPI
MsG0180004112.01	MsG0280006291.01	PPI
MsG0180006251.01	MsG0280006291.01	PPI
MsG0280006291.01	MsG0480018400.01	PPI
MsG0280006291.01	MsG0480022860.01	PPI
MsG0280006291.01	MsG0480018400.01	PPI
MsG0280006291.01	MsG0780039162.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006291.01.T01	MTR_2g005130	98.649	592	7	1	1	591	1	592	0.0	1197
MsG0280006291.01.T01	MTR_1g085090	40.109	551	313	9	39	577	18	563	5.77e-143	427
MsG0280006291.01.T01	MTR_2g017750	38.917	591	325	10	16	572	10	598	1.18e-142	426
MsG0280006291.01.T01	MTR_7g111260	41.682	535	295	8	39	562	18	546	1.22e-139	418
MsG0280006291.01.T01	MTR_8g087810	41.136	581	310	7	21	569	2	582	4.79e-139	417
MsG0280006291.01.T01	MTR_3g100980	39.746	551	300	8	34	562	10	550	9.16e-137	410
MsG0280006291.01.T01	MTR_8g087780	39.547	574	316	6	21	563	2	575	8.58e-136	408
MsG0280006291.01.T01	MTR_3g462710	40.000	565	312	11	26	568	10	569	3.47e-135	406
MsG0280006291.01.T01	MTR_3g100980	37.824	579	300	9	34	562	10	578	2.11e-130	395
MsG0280006291.01.T01	MTR_5g038380	39.716	564	304	8	29	562	17	574	8.78e-129	390

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006291.01.T01	AT1G59740	71.927	545	139	4	28	558	29	573	0.0	815
MsG0280006291.01.T01	AT1G33440	66.318	573	179	5	18	579	16	585	0.0	777
MsG0280006291.01.T01	AT1G69850	40.105	571	302	9	30	564	15	581	2.01e-131	397
MsG0280006291.01.T01	AT3G54140	36.491	570	335	7	18	565	2	566	8.49e-128	387
MsG0280006291.01.T01	AT3G54140	36.491	570	335	7	18	565	2	566	8.49e-128	387
MsG0280006291.01.T01	AT1G27040	39.927	551	305	9	30	561	13	556	4.24e-127	385
MsG0280006291.01.T01	AT1G27040	39.927	551	305	9	30	561	17	560	4.74e-127	385
MsG0280006291.01.T01	AT5G01180	36.649	573	327	9	18	564	3	565	1.68e-125	381
MsG0280006291.01.T01	AT5G01180	36.649	573	327	9	18	564	3	565	1.68e-125	381
MsG0280006291.01.T01	AT5G01180	36.649	573	327	9	18	564	3	565	1.68e-125	381

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Find 149 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATAACAGGTCATGAGTC+AGG	0.457304	2:-15247	MsG0280006291.01.T01:CDS
AAAATCACTTCCACTTCAAG+TGG	0.593401	2:-14920	MsG0280006291.01.T01:CDS
AAACCAAATATTATGAATTG+TGG	0.612544	2:+15088	None:intergenic
AAATATGAGGATAGTGGAGA+AGG	0.581400	2:+16454	None:intergenic
AACATAGTCACCAACTTTGT+AGG	0.426437	2:-16533	MsG0280006291.01.T01:CDS
AACCATATCCCAGGAGGGTG+AGG	0.505224	2:-14756	MsG0280006291.01.T01:CDS
AACCTCACCCTCCTGGGATA+TGG	0.323179	2:+14754	None:intergenic
AACTCATTCCGGGATGGATG+CGG	0.485644	2:-16011	MsG0280006291.01.T01:CDS
AAGCCCAACATGATTTCTCA+TGG	0.512660	2:-16151	MsG0280006291.01.T01:CDS
AAGGGAGAATCTGCAGATGA+AGG	0.539395	2:-16814	MsG0280006291.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAACGATGAGAAAAATGTA+AGG	+	Chr2:16105-16124	None:intergenic	30.0%
ACATATTGCTCCTATTCATT+TGG	-	Chr2:16432-16451	MsG0280006291.01.T01:intron	30.0%
ATAGTAGTGTTTAGATTCCA+TGG	+	Chr2:16688-16707	None:intergenic	30.0%
CGTTTATGAGATAAGTTATG+AGG	+	Chr2:14830-14849	None:intergenic	30.0%
CTATATAGCAATATACATGG+TGG	-	Chr2:15218-15237	MsG0280006291.01.T01:CDS	30.0%
CTGATGAATGATATATGTCA+GGG	-	Chr2:14760-14779	MsG0280006291.01.T01:CDS	30.0%
GTTCTAGTAAAGCAAATATG+AGG	+	Chr2:14974-14993	None:intergenic	30.0%
TAAAGCAAATATGAGGATAG+TGG	+	Chr2:14967-14986	None:intergenic	30.0%
TACAACATAGTGATAAGTTG+AGG	-	Chr2:15836-15855	MsG0280006291.01.T01:intron	30.0%
TAGAAAATGTAGCCAGAAAA+AGG	+	Chr2:16221-16240	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	14519	16915	14519	ID=MsG0280006291.01;Name=MsG0280006291.01
Chr2	mRNA	14519	16915	14519	ID=MsG0280006291.01.T01;Parent=MsG0280006291.01;Name=MsG0280006291.01.T01;_AED=0.15;_eAED=0.15;_QI=34\|1\|1\|1\|1\|1\|4\|181\|591
Chr2	exon	14519	15674	14519	ID=MsG0280006291.01.T01:exon:412;Parent=MsG0280006291.01.T01
Chr2	exon	15898	16326	15898	ID=MsG0280006291.01.T01:exon:411;Parent=MsG0280006291.01.T01
Chr2	exon	16436	16653	16436	ID=MsG0280006291.01.T01:exon:410;Parent=MsG0280006291.01.T01
Chr2	exon	16728	16915	16728	ID=MsG0280006291.01.T01:exon:409;Parent=MsG0280006291.01.T01
Chr2	five_prime_UTR	16882	16915	16882	ID=MsG0280006291.01.T01:five_prime_utr;Parent=MsG0280006291.01.T01
Chr2	CDS	16728	16881	16728	ID=MsG0280006291.01.T01:cds;Parent=MsG0280006291.01.T01
Chr2	CDS	16436	16653	16436	ID=MsG0280006291.01.T01:cds;Parent=MsG0280006291.01.T01
Chr2	CDS	15898	16326	15898	ID=MsG0280006291.01.T01:cds;Parent=MsG0280006291.01.T01
Chr2	CDS	14700	15674	14700	ID=MsG0280006291.01.T01:cds;Parent=MsG0280006291.01.T01
Chr2	three_prime_UTR	14519	14699	14519	ID=MsG0280006291.01.T01:three_prime_utr;Parent=MsG0280006291.01.T01

Gene Sequence

>MsG0280006291.01.T01

ATGGATGAGGGAGGAAGTATTAATAATATTAATATTCCAATTCCAAAGGGAGAATCTGCAGATGAAGGCATCATGAATACTACTGTTGACTGGAGAGGGAGACCCTCTAATCCTAACAACCACGGTGGAACTAGAGCAGCTGCTTTTGTTCTTGGGCTACAAGCATTTGAAATAATGGCAATAGCTGCAGTTGGAAACAACCTCATAACTTATCTCATAAACGAGATGCACTTCTCTTTGCCTCATTCTGCAAACATAGTCACCAACTTTGTAGGAACTATTTTTCTCATCGCACTCCTCGGTGGCTATCTATCTGACTCTTTTCTTGGCACCTTCTCCACTATCCTCATATTTGCTTTACTAGAACTTTCTGGTTTCATTTTGCTGTCAGCACAAGCTCATTTTCCTGAATTGAAGCCACCACCATGCAGTAAATTAGGAGGAGAATGCATAGAAGCAAAGGGGTTGAAGGCGTTGATATTCTATATAGCAATATACATGGTGGCATTAGGAAGTGGTTGTGTTAAGCCCAACATGATTTCTCATGGAGCTCACCAATTCAGTCAAGACCAGCAATCAAAGAAGCTCTCAACTTACTTCAATGCAGCATATTTTGCATTCTCATTGGGTGAACTTGTTGCTCTAACAATTCTTATTTGGGTTCAAACTCATTCCGGGATGGATGCGGGCTTTGCTGTATCAGCTGCTGCCATGGCAATGGGGTTCATAAGCTTCATAAGTGGGACCCTATATTATAAGAACAAACCTCCACAGCCAACCATCTTCCTCCCCATTGCTCAAGTTTTTGTAGCTGCAATATTAAAAAGGAAGAAGATTTGTCCATCTAGCCTACAAATGCTACACGGAAAGGAAAACATACAACATAGTGATAAGTTGAGGTTCTTGGACAAGGCTTGTGTCCAAGATGGGAACAACACAAAGGAAAGCCCGTGGAGATTGTGTAGTGTTGCACAAGTTGAACAAGCAAAGATATTACTTTCGGTTATTCCAATTTTTGCATGCACTATAATTTTCAACACTATCTTAGCACAACTTCAAACATTCTCGGTCCAGCAAGGAAGTGCCATGGACACACATGTCACAAAATCATTTCATATCCCTCCAGCATCACTTCAGTCAATTCCTTACATTTTTCTCATCGTTGTAGTCCCTCTCTATGATACTTTATTTGTTCCCTTTGCAAGAAAAATAACAGGTCATGAGTCAGGAATCTCACCTTTGCAGAGAATAGGGATTGGCCTTTTTCTGGCTACATTTTCTATGGTTTCAGCAGCTGTTATGGAGAAAAAGAGAAGGGATGCAGCTGTGAACCTGAACCAAACTCTGTCCATATTTTGGATTACACCACAATTCATAATATTTGGTTTGTCAGAGATGTTTACTGCAGTTGGCCTCATTGAGTTCTTCTACAAACAGTCCTTGAAAGGGATGCAGACATTCTTCACTGCCATCACATATTGCTCCTATTCATTTGGATTTTATCTCAGCTCATTATTGGTTTCTTTGGTTAATAAAATCACTTCCACTTCAAGTGGTGGTGGTGGTTGGCTTCATGACAACAATCTGAACAAAGACAAACTTGACCTTTTCTATTGGTTACTAGCTGTCCTCAGCTTCCTCAACTTCATCAACTATCTCTTCTGGTCCAGATGGTATTCTTATAATCCATCCCTTTCAACCATATCCCAGGAGGGTGAGGTTCATGCCATGGAATCTAAACACTACTATGTATCAGCAGCAGACAATATTCCATAG

Protein sequence

>MsG0280006291.01.T01

MDEGGSINNINIPIPKGESADEGIMNTTVDWRGRPSNPNNHGGTRAAAFVLGLQAFEIMAIAAVGNNLITYLINEMHFSLPHSANIVTNFVGTIFLIALLGGYLSDSFLGTFSTILIFALLELSGFILLSAQAHFPELKPPPCSKLGGECIEAKGLKALIFYIAIYMVALGSGCVKPNMISHGAHQFSQDQQSKKLSTYFNAAYFAFSLGELVALTILIWVQTHSGMDAGFAVSAAAMAMGFISFISGTLYYKNKPPQPTIFLPIAQVFVAAILKRKKICPSSLQMLHGKENIQHSDKLRFLDKACVQDGNNTKESPWRLCSVAQVEQAKILLSVIPIFACTIIFNTILAQLQTFSVQQGSAMDTHVTKSFHIPPASLQSIPYIFLIVVVPLYDTLFVPFARKITGHESGISPLQRIGIGLFLATFSMVSAAVMEKKRRDAAVNLNQTLSIFWITPQFIIFGLSEMFTAVGLIEFFYKQSLKGMQTFFTAITYCSYSFGFYLSSLLVSLVNKITSTSSGGGGWLHDNNLNKDKLDLFYWLLAVLSFLNFINYLFWSRWYSYNPSLSTISQEGEVHAMESKHYYVSAADNIP*