AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006364.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006364.01.T01 MTR_2g007000 91.250 80 6 1 1 79 1 80 7.83e-36 130
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 39 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAGAAGCATGATAGAGAT+AGG 0.366050 2:+941577 MsG0280006364.01.T01:CDS
GGAAAGAGAGAGGGAGAGAA+AGG 0.381291 2:+941771 MsG0280006364.01.T01:CDS
GGAAAGAGAGAGGGAGGAGA+AGG 0.418292 2:+941680 MsG0280006364.01.T01:CDS
AGAAGGAAAAGGAGAGAGTG+AGG 0.424132 2:+941697 MsG0280006364.01.T01:CDS
AGAGGAAGAGGGAAAGAGAG+AGG 0.438320 2:+941670 MsG0280006364.01.T01:CDS
GAGGGAGAGAAAGGAGGAGA+AGG 0.440578 2:+941780 MsG0280006364.01.T01:CDS
AGAAAGAGAGAGGGAAGAGA+AGG 0.447124 2:+941725 MsG0280006364.01.T01:CDS
AGAGAGGGAGGAGAAGGAAA+AGG 0.448290 2:+941686 MsG0280006364.01.T01:CDS
GAGAGGGAAGAGAAGGAGAA+AGG 0.449122 2:+941732 MsG0280006364.01.T01:CDS
AGGAGGGAAAACATAAATCT+AGG 0.459745 2:+941418 MsG0280006364.01.T01:CDS
GAAGAGGGAAAGAGAGAGGG+AGG 0.461041 2:+941674 MsG0280006364.01.T01:CDS
AAAACAGAGGGAGAGGAAGA+GGG 0.463179 2:+941659 MsG0280006364.01.T01:CDS
ATAGAGATAGGGAGAAGAGG+AGG 0.464250 2:+941589 MsG0280006364.01.T01:CDS
GAAGGAGAAAGGAGAGATTG+AGG 0.464547 2:+941743 MsG0280006364.01.T01:CDS
GAGGAAGAGGGAAAGAGAGA+GGG 0.469401 2:+941671 MsG0280006364.01.T01:CDS
AAGGAGAAAGGAGAGATTGA+GGG 0.481489 2:+941744 MsG0280006364.01.T01:CDS
GTAGTAGGCACAAGGATAAG+AGG 0.489402 2:+941508 MsG0280006364.01.T01:CDS
AAGAGAGAGGGAGAGAAAGG+AGG 0.492188 2:+941774 MsG0280006364.01.T01:CDS
TGAGGGAGAAAGAAAGAGAG+AGG 0.494655 2:+941715 MsG0280006364.01.T01:CDS
ATCGAAGCAGCGATGGTAGT+AGG 0.496341 2:+941493 MsG0280006364.01.T01:CDS
TAGAGATAGGGAGAAGAGGA+GGG 0.506429 2:+941590 MsG0280006364.01.T01:CDS
TGAGGGAGAAGGAAAGAGAG+AGG 0.509352 2:+941761 MsG0280006364.01.T01:CDS
ATGATAGAGATAGGGAGAAG+AGG 0.512878 2:+941586 MsG0280006364.01.T01:CDS
GAAGGAAAAGGAGAGAGTGA+GGG 0.516173 2:+941698 MsG0280006364.01.T01:CDS
GAGGAGGGAGAGTAAAGAGA+AGG 0.516899 2:+941605 MsG0280006364.01.T01:CDS
AAAGGAGAGATTGAGGGAGA+AGG 0.528495 2:+941750 MsG0280006364.01.T01:CDS
AGAGAAAGGAGGAGAAGGAG+AGG 0.529666 2:+941785 MsG0280006364.01.T01:CDS
GTAGGTTAAAGTAATGGAGG+AGG 0.531304 2:+941401 None:intergenic
AAGAAGATGCTGATTCTAAG+AGG 0.543493 2:+941442 MsG0280006364.01.T01:CDS
GAGGGAGAAAGAAAGAGAGA+GGG 0.547915 2:+941716 MsG0280006364.01.T01:CDS
TCGTAGTGATCGAGATCGTA+AGG 0.557740 2:+941470 MsG0280006364.01.T01:CDS
GGAGAGTAAAGAGAAGGAGA+AGG 0.573185 2:+941611 MsG0280006364.01.T01:CDS
GAGGGAGAAGGAAAGAGAGA+GGG 0.575167 2:+941762 MsG0280006364.01.T01:CDS
TAGGTTAAAGTAATGGAGGA+GGG 0.576108 2:+941402 None:intergenic
CGTAAGGATCGAAGCAGCGA+TGG 0.581013 2:+941486 MsG0280006364.01.T01:CDS
TGAGAAGCATGATAGAGATA+GGG 0.581706 2:+941578 MsG0280006364.01.T01:CDS
CAGCGATGGTAGTAGGCACA+AGG 0.620637 2:+941500 MsG0280006364.01.T01:CDS
TAGTAGGCACAAGGATAAGA+GGG 0.621895 2:+941509 MsG0280006364.01.T01:CDS
AGAGGGATCGTGAATCTCGA+AGG 0.627109 2:+941526 MsG0280006364.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGGAGGGAAAACATAAATCT+AGG + Chr2:941418-941437 MsG0280006364.01.T01:CDS 35.0%
! ATGAGAAGCATGATAGAGAT+AGG + Chr2:941577-941596 MsG0280006364.01.T01:CDS 35.0%
! TGAGAAGCATGATAGAGATA+GGG + Chr2:941578-941597 MsG0280006364.01.T01:CDS 35.0%
!! AAGAAGATGCTGATTCTAAG+AGG + Chr2:941442-941461 MsG0280006364.01.T01:CDS 35.0%
AAGGAGAAAGGAGAGATTGA+GGG + Chr2:941744-941763 MsG0280006364.01.T01:CDS 40.0%
ATGATAGAGATAGGGAGAAG+AGG + Chr2:941586-941605 MsG0280006364.01.T01:CDS 40.0%
ATGTCGAACGAGAAAAACAG+AGG + Chr2:941646-941665 MsG0280006364.01.T01:CDS 40.0%
TAGTAGGCACAAGGATAAGA+GGG + Chr2:941509-941528 MsG0280006364.01.T01:CDS 40.0%
TGTCGAACGAGAAAAACAGA+GGG + Chr2:941647-941666 MsG0280006364.01.T01:CDS 40.0%
AAAAACAGAGGGAGAGGAAG+AGG + Chr2:941658-941677 MsG0280006364.01.T01:CDS 45.0%
AAAACAGAGGGAGAGGAAGA+GGG + Chr2:941659-941678 MsG0280006364.01.T01:CDS 45.0%
AAAGGAGAGATTGAGGGAGA+AGG + Chr2:941750-941769 MsG0280006364.01.T01:CDS 45.0%
AACGAGAAAAACAGAGGGAG+AGG + Chr2:941652-941671 MsG0280006364.01.T01:CDS 45.0%
AGAAAGAGAGAGGGAAGAGA+AGG + Chr2:941725-941744 MsG0280006364.01.T01:CDS 45.0%
AGAAGGAAAAGGAGAGAGTG+AGG + Chr2:941697-941716 MsG0280006364.01.T01:CDS 45.0%
ATAGAGATAGGGAGAAGAGG+AGG + Chr2:941589-941608 MsG0280006364.01.T01:CDS 45.0%
GAAGGAAAAGGAGAGAGTGA+GGG + Chr2:941698-941717 MsG0280006364.01.T01:CDS 45.0%
GAAGGAGAAAGGAGAGATTG+AGG + Chr2:941743-941762 MsG0280006364.01.T01:CDS 45.0%
GAGGGAGAAAGAAAGAGAGA+GGG + Chr2:941716-941735 MsG0280006364.01.T01:CDS 45.0%
GGAGAGTAAAGAGAAGGAGA+AGG + Chr2:941611-941630 MsG0280006364.01.T01:CDS 45.0%
GTAGTAGGCACAAGGATAAG+AGG + Chr2:941508-941527 MsG0280006364.01.T01:CDS 45.0%
TAGAGATAGGGAGAAGAGGA+GGG + Chr2:941590-941609 MsG0280006364.01.T01:CDS 45.0%
TCGTAGTGATCGAGATCGTA+AGG + Chr2:941470-941489 MsG0280006364.01.T01:CDS 45.0%
TGAGGGAGAAAGAAAGAGAG+AGG + Chr2:941715-941734 MsG0280006364.01.T01:CDS 45.0%
AAGAGAGAGGGAGAGAAAGG+AGG + Chr2:941774-941793 MsG0280006364.01.T01:CDS 50.0%
AGAGAAAGGAGGAGAAGGAG+AGG + Chr2:941785-941804 MsG0280006364.01.T01:CDS 50.0%
AGAGAGGGAGGAGAAGGAAA+AGG + Chr2:941686-941705 MsG0280006364.01.T01:CDS 50.0%
AGAGGAAGAGGGAAAGAGAG+AGG + Chr2:941670-941689 MsG0280006364.01.T01:CDS 50.0%
AGAGGGATCGTGAATCTCGA+AGG + Chr2:941526-941545 MsG0280006364.01.T01:CDS 50.0%
ATCGAAGCAGCGATGGTAGT+AGG + Chr2:941493-941512 MsG0280006364.01.T01:CDS 50.0%
GAGAGGGAAGAGAAGGAGAA+AGG + Chr2:941732-941751 MsG0280006364.01.T01:CDS 50.0%
GAGGAAGAGGGAAAGAGAGA+GGG + Chr2:941671-941690 MsG0280006364.01.T01:CDS 50.0%
GAGGAGGGAGAGTAAAGAGA+AGG + Chr2:941605-941624 MsG0280006364.01.T01:CDS 50.0%
GAGGGAGAAGGAAAGAGAGA+GGG + Chr2:941762-941781 MsG0280006364.01.T01:CDS 50.0%
GGAAAGAGAGAGGGAGAGAA+AGG + Chr2:941771-941790 MsG0280006364.01.T01:CDS 50.0%
TGAGGGAGAAGGAAAGAGAG+AGG + Chr2:941761-941780 MsG0280006364.01.T01:CDS 50.0%
CAGCGATGGTAGTAGGCACA+AGG + Chr2:941500-941519 MsG0280006364.01.T01:CDS 55.0%
CGTAAGGATCGAAGCAGCGA+TGG + Chr2:941486-941505 MsG0280006364.01.T01:CDS 55.0%
GAAGAGGGAAAGAGAGAGGG+AGG + Chr2:941674-941693 MsG0280006364.01.T01:CDS 55.0%
GAGGGAGAGAAAGGAGGAGA+AGG + Chr2:941780-941799 MsG0280006364.01.T01:CDS 55.0%
GGAAAGAGAGAGGGAGGAGA+AGG + Chr2:941680-941699 MsG0280006364.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 941414 941812 941414 ID=MsG0280006364.01;Name=MsG0280006364.01
Chr2 mRNA 941414 941812 941414 ID=MsG0280006364.01.T01;Parent=MsG0280006364.01;Name=MsG0280006364.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|132
Chr2 exon 941414 941812 941414 ID=MsG0280006364.01.T01:exon:950;Parent=MsG0280006364.01.T01
Chr2 CDS 941414 941812 941414 ID=MsG0280006364.01.T01:cds;Parent=MsG0280006364.01.T01
Gene Sequence

>MsG0280006364.01.T01

ATGGAGGAGGGAAAACATAAATCTAGGAAAGAAGATGCTGATTCTAAGAGGAGTCATCGTAGTGATCGAGATCGTAAGGATCGAAGCAGCGATGGTAGTAGGCACAAGGATAAGAGGGATCGTGAATCTCGAAGGCACGATAGAGAGAAAAGTACTGATTCTGATGAGAAGCATGATAGAGATAGGGAGAAGAGGAGGGAGAGTAAAGAGAAGGAGAAGGCACGTGTACATGATGTCGAACGAGAAAAACAGAGGGAGAGGAAGAGGGAAAGAGAGAGGGAGGAGAAGGAAAAGGAGAGAGTGAGGGAGAAAGAAAGAGAGAGGGAAGAGAAGGAGAAAGGAGAGATTGAGGGAGAAGGAAAGAGAGAGGGAGAGAAAGGAGGAGAAGGAGAGGATTAG
Protein sequence

>MsG0280006364.01.T01

MEEGKHKSRKEDADSKRSHRSDRDRKDRSSDGSRHKDKRDRESRRHDREKSTDSDEKHDRDREKRRESKEKEKARVHDVEREKQRERKREREREEKEKERVREKEREREEKEKGEIEGEGKREGEKGGEGED*