AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006365.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006365.01.T01 MTR_2g007000 95.270 148 7 0 5 152 244 391 3.91e-88 279
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006365.01.T01 AT1G20920 50.000 152 69 4 4 152 271 418 2.49e-33 124
MsG0280006365.01.T01 AT1G20920 50.000 152 69 4 4 152 271 418 2.49e-33 124
MsG0280006365.01.T01 AT1G20920 50.000 152 69 4 4 152 271 418 2.49e-33 124
MsG0280006365.01.T01 AT1G20920 50.000 152 69 4 4 152 271 418 2.49e-33 124
MsG0280006365.01.T01 AT1G20920 50.000 152 69 4 4 152 271 418 2.49e-33 124
MsG0280006365.01.T01 AT1G20920 50.000 152 69 4 4 152 271 418 2.49e-33 124
MsG0280006365.01.T01 AT3G09620 53.333 135 52 4 1 135 150 273 1.31e-30 117
MsG0280006365.01.T01 AT3G09620 53.333 135 52 4 1 135 151 274 1.32e-30 117
MsG0280006365.01.T01 AT1G20920 45.783 83 42 2 70 152 1 80 6.13e-11 60.5

Find 50 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCTCAGCCCCTTTATCTT+TGG 0.239445 2:-942580 None:intergenic
GGATGCTTTCATGAATTCTA+TGG 0.277935 2:+942486 MsG0280006365.01.T01:CDS
TGACTTGAAACCAAAGATAA+AGG 0.304960 2:+942570 MsG0280006365.01.T01:CDS
GACTTGAAACCAAAGATAAA+GGG 0.385570 2:+942571 MsG0280006365.01.T01:CDS
AAAGAGGTTGGATGATGAAA+TGG 0.396497 2:+942153 MsG0280006365.01.T01:CDS
AAAGGTTCAAATAAATCTAT+TGG 0.398381 2:+942624 MsG0280006365.01.T01:CDS
GGAAAGAAATGGACACTTGA+TGG 0.399545 2:+942280 MsG0280006365.01.T01:CDS
AAAGGGGCTGAGAGTAGAAA+TGG 0.409817 2:+942588 MsG0280006365.01.T01:CDS
AACTGGTAAACATACGAGTA+TGG 0.417487 2:+942327 MsG0280006365.01.T01:CDS
CAACCTCCTTAGCTCTTGCC+AGG 0.434087 2:-942200 None:intergenic
GCTAAGGAGGTTGGAGGAAG+AGG 0.439921 2:+942210 MsG0280006365.01.T01:CDS
GTCCATTTCTTTCCAGACTC+AGG 0.458387 2:-942271 None:intergenic
AGTGTTGTTGAACCTGAGTC+TGG 0.462097 2:+942259 MsG0280006365.01.T01:CDS
GGAAGAGGAACAAAAGAGGT+TGG 0.473996 2:+942141 MsG0280006365.01.T01:CDS
TTCAAGCCTGGCAAGAGCTA+AGG 0.488305 2:+942194 MsG0280006365.01.T01:CDS
TAATGAACCTACTGATTCAA+TGG 0.497980 2:+942378 MsG0280006365.01.T01:CDS
GAAAGAAATGGACACTTGAT+GGG 0.499261 2:+942281 MsG0280006365.01.T01:CDS
GAATTCTATGGTTCTTCCTG+AGG 0.502548 2:+942498 MsG0280006365.01.T01:CDS
ATGAAATGGAAAAGCGGAGG+AGG 0.505342 2:+942167 MsG0280006365.01.T01:CDS
GTTGCGTCTGATTTACAGAA+TGG 0.512245 2:+942424 MsG0280006365.01.T01:CDS
GTTCAAATAAATCTATTGGC+AGG 0.524487 2:+942628 MsG0280006365.01.T01:CDS
GGGTCGATTTCATCCTCTGC+AGG 0.531563 2:-942460 None:intergenic
CCGTGAGGAGGAGATGGAAG+AGG 0.540602 2:+942126 None:intergenic
GTAGAAATGGTGGACAATCA+AGG 0.541523 2:+942601 MsG0280006365.01.T01:CDS
AACCTGAGTCTGGAAAGAAA+TGG 0.542330 2:+942269 MsG0280006365.01.T01:CDS
AATTCATGAAAGCATCCAAT+GGG 0.542396 2:-942480 None:intergenic
GCAAGAGCTAAGGAGGTTGG+AGG 0.544013 2:+942204 MsG0280006365.01.T01:CDS
TCTGATGATGAAGATGGAAC+TGG 0.544266 2:+942310 MsG0280006365.01.T01:CDS
AGATGGAAGAGGAACAAAAG+AGG 0.545916 2:+942137 None:intergenic
CTGGCAAGAGCTAAGGAGGT+TGG 0.552682 2:+942201 MsG0280006365.01.T01:CDS
GGAGGAGGAAAGTTCAAGCC+TGG 0.567121 2:+942182 MsG0280006365.01.T01:CDS
GAGGAATCTGATGATGAAGA+TGG 0.571097 2:+942304 MsG0280006365.01.T01:CDS
GATTTACAGAATGGAGATGC+TGG 0.593367 2:+942433 MsG0280006365.01.T01:CDS
AAGTCAGAAGCATTATCAGA+AGG 0.597656 2:-942553 None:intergenic
AAGCCTGGCAAGAGCTAAGG+AGG 0.599145 2:+942197 MsG0280006365.01.T01:CDS
AGAGGATGAAATCGACCCAT+TGG 0.601106 2:+942465 MsG0280006365.01.T01:CDS
GAATTCATGAAAGCATCCAA+TGG 0.613023 2:-942481 None:intergenic
AATGGTGGACAATCAAGGAA+AGG 0.615667 2:+942606 MsG0280006365.01.T01:CDS
ACTTGAAACCAAAGATAAAG+GGG 0.623570 2:+942572 MsG0280006365.01.T01:CDS
GAAATGGACACTTGATGGGG+AGG 0.625730 2:+942285 MsG0280006365.01.T01:CDS
TGGATGATGAAATGGAAAAG+CGG 0.633003 2:+942161 MsG0280006365.01.T01:CDS
AGATGCTGGTGCTCCTGCAG+AGG 0.650459 2:+942447 MsG0280006365.01.T01:CDS
GGGGCTGAGAGTAGAAATGG+TGG 0.657026 2:+942591 MsG0280006365.01.T01:CDS
TCTACCGCCATTGAATCAGT+AGG 0.659454 2:-942385 None:intergenic
AATGGCGGTAGATGTTGACA+AGG 0.661828 2:+942396 MsG0280006365.01.T01:CDS
AAAGAAATGGACACTTGATG+GGG 0.672554 2:+942282 MsG0280006365.01.T01:CDS
ATGATGAAATGGAAAAGCGG+AGG 0.700220 2:+942164 MsG0280006365.01.T01:CDS
TGAACCTACTGATTCAATGG+CGG 0.715854 2:+942381 MsG0280006365.01.T01:CDS
AGTATGGATATAGACGAAGA+CGG 0.738970 2:+942343 MsG0280006365.01.T01:CDS
ATGGCGGTAGATGTTGACAA+GGG 0.741446 2:+942397 MsG0280006365.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGGTTCAAATAAATCTAT+TGG + Chr2:942624-942643 MsG0280006365.01.T01:CDS 20.0%
ACTTGAAACCAAAGATAAAG+GGG + Chr2:942572-942591 MsG0280006365.01.T01:CDS 30.0%
GACTTGAAACCAAAGATAAA+GGG + Chr2:942571-942590 MsG0280006365.01.T01:CDS 30.0%
GTTCAAATAAATCTATTGGC+AGG + Chr2:942628-942647 MsG0280006365.01.T01:CDS 30.0%
TGACTTGAAACCAAAGATAA+AGG + Chr2:942570-942589 MsG0280006365.01.T01:CDS 30.0%
! TTATTAAGCTTTTCAACCTC+AGG - Chr2:942517-942536 None:intergenic 30.0%
!! AATTCATGAAAGCATCCAAT+GGG - Chr2:942483-942502 None:intergenic 30.0%
!! TAATGAACCTACTGATTCAA+TGG + Chr2:942378-942397 MsG0280006365.01.T01:CDS 30.0%
AAAGAAATGGACACTTGATG+GGG + Chr2:942282-942301 MsG0280006365.01.T01:CDS 35.0%
AAAGAGGTTGGATGATGAAA+TGG + Chr2:942153-942172 MsG0280006365.01.T01:CDS 35.0%
AACTGGTAAACATACGAGTA+TGG + Chr2:942327-942346 MsG0280006365.01.T01:CDS 35.0%
AGTATGGATATAGACGAAGA+CGG + Chr2:942343-942362 MsG0280006365.01.T01:CDS 35.0%
GAAAGAAATGGACACTTGAT+GGG + Chr2:942281-942300 MsG0280006365.01.T01:CDS 35.0%
GAGGCACAAAGAAAAAAACA+AGG + Chr2:942229-942248 MsG0280006365.01.T01:CDS 35.0%
GGATGCTTTCATGAATTCTA+TGG + Chr2:942486-942505 MsG0280006365.01.T01:CDS 35.0%
TGGATGATGAAATGGAAAAG+CGG + Chr2:942161-942180 MsG0280006365.01.T01:CDS 35.0%
!! AAGTCAGAAGCATTATCAGA+AGG - Chr2:942556-942575 None:intergenic 35.0%
!! GAATTCATGAAAGCATCCAA+TGG - Chr2:942484-942503 None:intergenic 35.0%
AACCTGAGTCTGGAAAGAAA+TGG + Chr2:942269-942288 MsG0280006365.01.T01:CDS 40.0%
ATGATGAAATGGAAAAGCGG+AGG + Chr2:942164-942183 MsG0280006365.01.T01:CDS 40.0%
GAATTCTATGGTTCTTCCTG+AGG + Chr2:942498-942517 MsG0280006365.01.T01:CDS 40.0%
GATTTACAGAATGGAGATGC+TGG + Chr2:942433-942452 MsG0280006365.01.T01:CDS 40.0%
GGAAAGAAATGGACACTTGA+TGG + Chr2:942280-942299 MsG0280006365.01.T01:CDS 40.0%
GTTGCGTCTGATTTACAGAA+TGG + Chr2:942424-942443 MsG0280006365.01.T01:CDS 40.0%
! GAGGAATCTGATGATGAAGA+TGG + Chr2:942304-942323 MsG0280006365.01.T01:CDS 40.0%
! TCTGATGATGAAGATGGAAC+TGG + Chr2:942310-942329 MsG0280006365.01.T01:CDS 40.0%
!! AATGGTGGACAATCAAGGAA+AGG + Chr2:942606-942625 MsG0280006365.01.T01:CDS 40.0%
!! GTAGAAATGGTGGACAATCA+AGG + Chr2:942601-942620 MsG0280006365.01.T01:CDS 40.0%
!! TGAACCTACTGATTCAATGG+CGG + Chr2:942381-942400 MsG0280006365.01.T01:CDS 40.0%
AAAGGGGCTGAGAGTAGAAA+TGG + Chr2:942588-942607 MsG0280006365.01.T01:CDS 45.0%
AATGGCGGTAGATGTTGACA+AGG + Chr2:942396-942415 MsG0280006365.01.T01:CDS 45.0%
ACTCTCAGCCCCTTTATCTT+TGG - Chr2:942583-942602 None:intergenic 45.0%
AGAGGATGAAATCGACCCAT+TGG + Chr2:942465-942484 MsG0280006365.01.T01:CDS 45.0%
ATGAAATGGAAAAGCGGAGG+AGG + Chr2:942167-942186 MsG0280006365.01.T01:CDS 45.0%
ATGGCGGTAGATGTTGACAA+GGG + Chr2:942397-942416 MsG0280006365.01.T01:CDS 45.0%
GGAAGAGGAACAAAAGAGGT+TGG + Chr2:942141-942160 MsG0280006365.01.T01:CDS 45.0%
GTCCATTTCTTTCCAGACTC+AGG - Chr2:942274-942293 None:intergenic 45.0%
TCTACCGCCATTGAATCAGT+AGG - Chr2:942388-942407 None:intergenic 45.0%
! AGTGTTGTTGAACCTGAGTC+TGG + Chr2:942259-942278 MsG0280006365.01.T01:CDS 45.0%
GAAATGGACACTTGATGGGG+AGG + Chr2:942285-942304 MsG0280006365.01.T01:CDS 50.0%
TTCAAGCCTGGCAAGAGCTA+AGG + Chr2:942194-942213 MsG0280006365.01.T01:CDS 50.0%
AAGCCTGGCAAGAGCTAAGG+AGG + Chr2:942197-942216 MsG0280006365.01.T01:CDS 55.0%
CTGGCAAGAGCTAAGGAGGT+TGG + Chr2:942201-942220 MsG0280006365.01.T01:CDS 55.0%
GCAAGAGCTAAGGAGGTTGG+AGG + Chr2:942204-942223 MsG0280006365.01.T01:CDS 55.0%
GCTAAGGAGGTTGGAGGAAG+AGG + Chr2:942210-942229 MsG0280006365.01.T01:CDS 55.0%
GGAGGAGGAAAGTTCAAGCC+TGG + Chr2:942182-942201 MsG0280006365.01.T01:CDS 55.0%
GGGGCTGAGAGTAGAAATGG+TGG + Chr2:942591-942610 MsG0280006365.01.T01:CDS 55.0%
! CAACCTCCTTAGCTCTTGCC+AGG - Chr2:942203-942222 None:intergenic 55.0%
! GGGTCGATTTCATCCTCTGC+AGG - Chr2:942463-942482 None:intergenic 55.0%
AGATGCTGGTGCTCCTGCAG+AGG + Chr2:942447-942466 MsG0280006365.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 942139 942654 942139 ID=MsG0280006365.01;Name=MsG0280006365.01
Chr2 mRNA 942139 942654 942139 ID=MsG0280006365.01.T01;Parent=MsG0280006365.01;Name=MsG0280006365.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|171
Chr2 exon 942139 942654 942139 ID=MsG0280006365.01.T01:exon:412;Parent=MsG0280006365.01.T01
Chr2 CDS 942139 942654 942139 ID=MsG0280006365.01.T01:cds;Parent=MsG0280006365.01.T01
Gene Sequence

>MsG0280006365.01.T01

ATGGAAGAGGAACAAAAGAGGTTGGATGATGAAATGGAAAAGCGGAGGAGGAAAGTTCAAGCCTGGCAAGAGCTAAGGAGGTTGGAGGAAGAGGCACAAAGAAAAAAACAAGGTGAAGCAAGTGTTGTTGAACCTGAGTCTGGAAAGAAATGGACACTTGATGGGGAGGAATCTGATGATGAAGATGGAACTGGTAAACATACGAGTATGGATATAGACGAAGACGGTAAACTTGCTGATAATGAACCTACTGATTCAATGGCGGTAGATGTTGACAAGGGAACTGTTGCGTCTGATTTACAGAATGGAGATGCTGGTGCTCCTGCAGAGGATGAAATCGACCCATTGGATGCTTTCATGAATTCTATGGTTCTTCCTGAGGTTGAAAAGCTTAATAATGCTGTTAACTCAGCACCTTCTGATAATGCTTCTGACTTGAAACCAAAGATAAAGGGGCTGAGAGTAGAAATGGTGGACAATCAAGGAAAGGTTCAAATAAATCTATTGGCAGGATAA

Protein sequence

>MsG0280006365.01.T01

MEEEQKRLDDEMEKRRRKVQAWQELRRLEEEAQRKKQGEASVVEPESGKKWTLDGEESDDEDGTGKHTSMDIDEDGKLADNEPTDSMAVDVDKGTVASDLQNGDAGAPAEDEIDPLDAFMNSMVLPEVEKLNNAVNSAPSDNASDLKPKIKGLRVEMVDNQGKVQINLLAG*