Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006421.01.T01 | XP_013442645.1 | 99.476 | 191 | 1 | 0 | 1 | 191 | 22 | 212 | 2.85E-133 | 382 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006421.01.T01 | Q9M2C0 | 70.588 | 187 | 51 | 2 | 1 | 186 | 10 | 193 | 2.94E-89 | 263 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006421.01.T01 | A0A072TSY7 | 99.476 | 191 | 1 | 0 | 1 | 191 | 22 | 212 | 1.36e-133 | 382 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000567.01 | MsG0280006421.01 | 0.802562 | 5.288537e-49 | 2.598715e-46 |
| MsG0280006421.01 | MsG0880042843.01 | 0.809653 | 1.701729e-50 | 1.004893e-47 |
| MsG0280006421.01 | MsG0880042967.01 | 0.801337 | 9.442612e-49 | 4.498031e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006421.01.T01 | MTR_0119s0010 | 99.476 | 191 | 1 | 0 | 1 | 191 | 22 | 212 | 3.46e-137 | 382 |
| MsG0280006421.01.T01 | MTR_0119s0020 | 99.476 | 191 | 1 | 0 | 1 | 191 | 22 | 212 | 3.46e-137 | 382 |
| MsG0280006421.01.T01 | MTR_2g008110 | 99.476 | 191 | 1 | 0 | 1 | 191 | 22 | 212 | 3.81e-137 | 382 |
| MsG0280006421.01.T01 | MTR_1g099010 | 70.652 | 184 | 49 | 1 | 8 | 186 | 29 | 212 | 7.45e-87 | 254 |
| MsG0280006421.01.T01 | MTR_6g034975 | 66.667 | 195 | 55 | 2 | 2 | 186 | 11 | 205 | 1.77e-76 | 228 |
| MsG0280006421.01.T01 | MTR_4g094328 | 60.938 | 192 | 66 | 1 | 6 | 188 | 30 | 221 | 1.64e-75 | 226 |
| MsG0280006421.01.T01 | MTR_4g094330 | 60.938 | 192 | 66 | 1 | 6 | 188 | 30 | 221 | 1.64e-75 | 226 |
| MsG0280006421.01.T01 | MTR_4g094332 | 61.579 | 190 | 64 | 1 | 6 | 186 | 30 | 219 | 2.09e-75 | 226 |
| MsG0280006421.01.T01 | MTR_4g094325 | 59.391 | 197 | 70 | 2 | 2 | 188 | 40 | 236 | 2.34e-72 | 219 |
| MsG0280006421.01.T01 | MTR_4g094335 | 54.000 | 200 | 80 | 1 | 2 | 189 | 29 | 228 | 6.81e-63 | 194 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006421.01.T01 | AT3G43660 | 70.588 | 187 | 51 | 2 | 1 | 186 | 10 | 193 | 3.00e-90 | 263 |
| MsG0280006421.01.T01 | AT3G43630 | 73.481 | 181 | 45 | 2 | 9 | 186 | 12 | 192 | 2.49e-84 | 248 |
| MsG0280006421.01.T01 | AT1G21140 | 73.481 | 181 | 45 | 2 | 9 | 186 | 15 | 195 | 2.18e-83 | 245 |
| MsG0280006421.01.T01 | AT1G76800 | 68.817 | 186 | 54 | 1 | 1 | 186 | 10 | 191 | 1.29e-77 | 231 |
| MsG0280006421.01.T01 | AT3G25190 | 59.091 | 198 | 68 | 2 | 2 | 186 | 15 | 212 | 6.80e-64 | 196 |
Find 39 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGCTATGTCTCTTACTTT+TGG | 0.225220 | 2:+1760314 | MsG0280006421.01.T01:CDS |
| AGCCTTGCTTTGTTTGCTTT+TGG | 0.248729 | 2:+1760221 | MsG0280006421.01.T01:CDS |
| GAAGCAAAGGCAGCATAATA+AGG | 0.258027 | 2:-1760101 | None:intergenic |
| GGCTCCAACACCCATCATTA+AGG | 0.342144 | 2:-1759910 | None:intergenic |
| TGTTCTTGGGAAAGCTCCTT+TGG | 0.348881 | 2:+1760256 | MsG0280006421.01.T01:CDS |
| TTTGGTGAAGTCTTCTTTGA+GGG | 0.356177 | 2:+1760274 | MsG0280006421.01.T01:CDS |
| GTTAAGACAATGATTCTAAC+TGG | 0.372301 | 2:+1759945 | MsG0280006421.01.T01:CDS |
| GGATTGTTGAGTGCTGTTCT+TGG | 0.377078 | 2:+1760242 | MsG0280006421.01.T01:CDS |
| CTCAAAGAGCACAATGGCTT+AGG | 0.396117 | 2:+1759844 | MsG0280006421.01.T01:CDS |
| TTATTATGCTGCCTTTGCTT+CGG | 0.407157 | 2:+1760103 | MsG0280006421.01.T01:CDS |
| CTTTGGTGAAGTCTTCTTTG+AGG | 0.408112 | 2:+1760273 | MsG0280006421.01.T01:CDS |
| ATGATTCTAACTGGAATTGC+TGG | 0.418092 | 2:+1759954 | MsG0280006421.01.T01:CDS |
| GATTGTTGAGTGCTGTTCTT+GGG | 0.419290 | 2:+1760243 | MsG0280006421.01.T01:CDS |
| GGCGTTTGTTAAAGACTATA+AGG | 0.421985 | 2:+1760169 | MsG0280006421.01.T01:CDS |
| GGCGCCTTTGTGCCGCTACT+CGG | 0.427583 | 2:+1760143 | MsG0280006421.01.T01:CDS |
| CTCAACAGCATCCTTAATGA+TGG | 0.435656 | 2:+1759899 | MsG0280006421.01.T01:CDS |
| TAAAGACTATAAGGTGAGGT+TGG | 0.443344 | 2:+1760178 | MsG0280006421.01.T01:CDS |
| GGAATTGCTGGTCTTGTAGC+TGG | 0.462496 | 2:+1759966 | MsG0280006421.01.T01:CDS |
| GTGAGGTTGGGAGTTGTGGT+TGG | 0.470924 | 2:+1760191 | MsG0280006421.01.T01:CDS |
| AAAAGCGATGGCCGAAGCAA+AGG | 0.483514 | 2:-1760114 | None:intergenic |
| TCAAAGAGCACAATGGCTTA+GGG | 0.493819 | 2:+1759845 | MsG0280006421.01.T01:CDS |
| TAAGGTGAGGTTGGGAGTTG+TGG | 0.497331 | 2:+1760187 | MsG0280006421.01.T01:CDS |
| AAAGACTATAAGGTGAGGTT+GGG | 0.502612 | 2:+1760179 | MsG0280006421.01.T01:CDS |
| AAGCAAAGGCAGCATAATAA+GGG | 0.516802 | 2:-1760100 | None:intergenic |
| GGCGCCGACCGCAAAAGCGA+TGG | 0.528738 | 2:-1760126 | None:intergenic |
| GCATCCTTAATGATGGGTGT+TGG | 0.549500 | 2:+1759906 | MsG0280006421.01.T01:CDS |
| ATCCAAAAGCAAACAAAGCA+AGG | 0.566512 | 2:-1760223 | None:intergenic |
| AAGGCAGCATAATAAGGGTT+AGG | 0.567356 | 2:-1760095 | None:intergenic |
| CGCGCCGAGTAGCGGCACAA+AGG | 0.568749 | 2:-1760147 | None:intergenic |
| GGTGCATGTAGCATGGCAAT+TGG | 0.569674 | 2:+1759987 | MsG0280006421.01.T01:CDS |
| TCAACAGCATCCTTAATGAT+GGG | 0.587391 | 2:+1759900 | MsG0280006421.01.T01:CDS |
| ACAAACGCCGCGCCGAGTAG+CGG | 0.601438 | 2:-1760155 | None:intergenic |
| CTCAAAGAAGACTTCACCAA+AGG | 0.626739 | 2:-1760272 | None:intergenic |
| TGTAGCTGGTGCATGTAGCA+TGG | 0.629799 | 2:+1759980 | MsG0280006421.01.T01:CDS |
| TTGTTAAAGACTATAAGGTG+AGG | 0.635278 | 2:+1760174 | MsG0280006421.01.T01:CDS |
| CTTTGTGCCGCTACTCGGCG+CGG | 0.645709 | 2:+1760148 | MsG0280006421.01.T01:CDS |
| ATTATACTCAAAGAGCACAA+TGG | 0.665811 | 2:+1759838 | MsG0280006421.01.T01:CDS |
| TTTGCACAAATGAAAAGACA+AGG | 0.695742 | 2:+1760047 | MsG0280006421.01.T01:CDS |
| AAAATGATCAAACTACAACA+TGG | 0.718736 | 2:-1760355 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGTCTTAACATCTTTTGTAA+CGG | - | Chr2:1759934-1759953 | None:intergenic | 25.0% |
| GTTAAGACAATGATTCTAAC+TGG | + | Chr2:1759945-1759964 | MsG0280006421.01.T01:CDS | 30.0% | |
| TTGTTAAAGACTATAAGGTG+AGG | + | Chr2:1760174-1760193 | MsG0280006421.01.T01:CDS | 30.0% | |
| TTTGCACAAATGAAAAGACA+AGG | + | Chr2:1760047-1760066 | MsG0280006421.01.T01:CDS | 30.0% | |
| ! | ATTATACTCAAAGAGCACAA+TGG | + | Chr2:1759838-1759857 | MsG0280006421.01.T01:CDS | 30.0% |
| AAAGACTATAAGGTGAGGTT+GGG | + | Chr2:1760179-1760198 | MsG0280006421.01.T01:CDS | 35.0% | |
| AAGCAAAGGCAGCATAATAA+GGG | - | Chr2:1760103-1760122 | None:intergenic | 35.0% | |
| ATCCAAAAGCAAACAAAGCA+AGG | - | Chr2:1760226-1760245 | None:intergenic | 35.0% | |
| ATGATTCTAACTGGAATTGC+TGG | + | Chr2:1759954-1759973 | MsG0280006421.01.T01:CDS | 35.0% | |
| TAAAGACTATAAGGTGAGGT+TGG | + | Chr2:1760178-1760197 | MsG0280006421.01.T01:CDS | 35.0% | |
| TCAACAGCATCCTTAATGAT+GGG | + | Chr2:1759900-1759919 | MsG0280006421.01.T01:CDS | 35.0% | |
| TTATTATGCTGCCTTTGCTT+CGG | + | Chr2:1760103-1760122 | MsG0280006421.01.T01:CDS | 35.0% | |
| TTTGGTGAAGTCTTCTTTGA+GGG | + | Chr2:1760274-1760293 | MsG0280006421.01.T01:CDS | 35.0% | |
| ! | GGCGTTTGTTAAAGACTATA+AGG | + | Chr2:1760169-1760188 | MsG0280006421.01.T01:CDS | 35.0% |
| !! | CTTGCTATGTCTCTTACTTT+TGG | + | Chr2:1760314-1760333 | MsG0280006421.01.T01:CDS | 35.0% |
| !! | TCTTCTTTGAGGGTTTTGAT+TGG | + | Chr2:1760284-1760303 | MsG0280006421.01.T01:CDS | 35.0% |
| AAGGCAGCATAATAAGGGTT+AGG | - | Chr2:1760098-1760117 | None:intergenic | 40.0% | |
| CTCAAAGAAGACTTCACCAA+AGG | - | Chr2:1760275-1760294 | None:intergenic | 40.0% | |
| CTCAACAGCATCCTTAATGA+TGG | + | Chr2:1759899-1759918 | MsG0280006421.01.T01:CDS | 40.0% | |
| CTTTGGTGAAGTCTTCTTTG+AGG | + | Chr2:1760273-1760292 | MsG0280006421.01.T01:CDS | 40.0% | |
| GAAGCAAAGGCAGCATAATA+AGG | - | Chr2:1760104-1760123 | None:intergenic | 40.0% | |
| ! | GATTGTTGAGTGCTGTTCTT+GGG | + | Chr2:1760243-1760262 | MsG0280006421.01.T01:CDS | 40.0% |
| ! | TCAAAGAGCACAATGGCTTA+GGG | + | Chr2:1759845-1759864 | MsG0280006421.01.T01:CDS | 40.0% |
| !! | GCTGTTTTAGGTGCTAATGA+TGG | + | Chr2:1759870-1759889 | MsG0280006421.01.T01:CDS | 40.0% |
| !!! | AGCCTTGCTTTGTTTGCTTT+TGG | + | Chr2:1760221-1760240 | MsG0280006421.01.T01:CDS | 40.0% |
| !!! | TCTTTGAGGGTTTTGATTGG+TGG | + | Chr2:1760287-1760306 | MsG0280006421.01.T01:CDS | 40.0% |
| TGTTCTTGGGAAAGCTCCTT+TGG | + | Chr2:1760256-1760275 | MsG0280006421.01.T01:CDS | 45.0% | |
| ! | CTCAAAGAGCACAATGGCTT+AGG | + | Chr2:1759844-1759863 | MsG0280006421.01.T01:CDS | 45.0% |
| ! | GGATTGTTGAGTGCTGTTCT+TGG | + | Chr2:1760242-1760261 | MsG0280006421.01.T01:CDS | 45.0% |
| !! | GCATCCTTAATGATGGGTGT+TGG | + | Chr2:1759906-1759925 | MsG0280006421.01.T01:CDS | 45.0% |
| !!! | TGAGGGTTTTGATTGGTGGA+TGG | + | Chr2:1760291-1760310 | MsG0280006421.01.T01:CDS | 45.0% |
| AAAAGCGATGGCCGAAGCAA+AGG | - | Chr2:1760117-1760136 | None:intergenic | 50.0% | |
| GGCTCCAACACCCATCATTA+AGG | - | Chr2:1759913-1759932 | None:intergenic | 50.0% | |
| GGTGCATGTAGCATGGCAAT+TGG | + | Chr2:1759987-1760006 | MsG0280006421.01.T01:CDS | 50.0% | |
| TAAGGTGAGGTTGGGAGTTG+TGG | + | Chr2:1760187-1760206 | MsG0280006421.01.T01:CDS | 50.0% | |
| TGTAGCTGGTGCATGTAGCA+TGG | + | Chr2:1759980-1759999 | MsG0280006421.01.T01:CDS | 50.0% | |
| !! | GGAATTGCTGGTCTTGTAGC+TGG | + | Chr2:1759966-1759985 | MsG0280006421.01.T01:CDS | 50.0% |
| !! | TGGCTTAGGGCAGCTGTTTT+AGG | + | Chr2:1759858-1759877 | MsG0280006421.01.T01:CDS | 50.0% |
| GTGAGGTTGGGAGTTGTGGT+TGG | + | Chr2:1760191-1760210 | MsG0280006421.01.T01:CDS | 55.0% | |
| !! | TGCTTCGGCCATCGCTTTTG+CGG | + | Chr2:1760118-1760137 | MsG0280006421.01.T01:CDS | 55.0% |
| !! | TCGGCCATCGCTTTTGCGGT+CGG | + | Chr2:1760122-1760141 | MsG0280006421.01.T01:CDS | 60.0% |
| ACAAACGCCGCGCCGAGTAG+CGG | - | Chr2:1760158-1760177 | None:intergenic | 65.0% | |
| ! | CTTTGTGCCGCTACTCGGCG+CGG | + | Chr2:1760148-1760167 | MsG0280006421.01.T01:CDS | 65.0% |
| ! | GGCGCCTTTGTGCCGCTACT+CGG | + | Chr2:1760143-1760162 | MsG0280006421.01.T01:CDS | 65.0% |
| CGCGCCGAGTAGCGGCACAA+AGG | - | Chr2:1760150-1760169 | None:intergenic | 70.0% | |
| !! | GGCGCCGACCGCAAAAGCGA+TGG | - | Chr2:1760129-1760148 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 1759795 | 1760370 | 1759795 | ID=MsG0280006421.01;Name=MsG0280006421.01 |
| Chr2 | mRNA | 1759795 | 1760370 | 1759795 | ID=MsG0280006421.01.T01;Parent=MsG0280006421.01;Name=MsG0280006421.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|191 |
| Chr2 | exon | 1759795 | 1760370 | 1759795 | ID=MsG0280006421.01.T01:exon:163;Parent=MsG0280006421.01.T01 |
| Chr2 | CDS | 1759795 | 1760370 | 1759795 | ID=MsG0280006421.01.T01:cds;Parent=MsG0280006421.01.T01 |
| Gene Sequence |
| Protein sequence |
