AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880042843.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042843.01.T01 MTR_7g117540 57.447 47 20 0 34 80 101 147 2.97e-15 70.5
MsG0880042843.01.T01 MTR_1g043410 45.833 72 34 1 9 80 103 169 7.21e-15 69.7
MsG0880042843.01.T01 MTR_6g007670 38.554 83 50 1 5 86 142 224 1.11e-14 68.6
MsG0880042843.01.T01 MTR_6g007670 41.509 53 31 0 34 86 33 85 1.01e-12 63.2
MsG0880042843.01.T01 MTR_7g116210 50.000 50 25 0 28 77 29 78 4.00e-14 64.3
MsG0880042843.01.T01 MTR_7g116200 50.000 50 25 0 28 77 87 136 5.43e-14 65.1
MsG0880042843.01.T01 MTR_8g093010 58.140 43 18 0 34 76 134 176 5.77e-14 66.2
MsG0880042843.01.T01 MTR_5g082940 54.000 50 22 1 30 79 100 148 8.08e-14 64.7
MsG0880042843.01.T01 MTR_5g026730 32.979 94 59 1 10 103 74 163 9.43e-14 64.7
MsG0880042843.01.T01 MTR_8g066780 52.632 57 25 1 34 88 142 198 1.32e-13 66.2
MsG0880042843.01.T01 MTR_8g063660 50.000 50 25 0 34 83 94 143 1.74e-13 65.9
MsG0880042843.01.T01 MTR_6g007680 36.585 82 50 2 6 86 38 118 2.43e-13 63.2
MsG0880042843.01.T01 MTR_1g070140 50.000 52 26 0 34 85 130 181 4.49e-13 64.7
MsG0880042843.01.T01 MTR_1g074190 48.276 58 29 1 24 80 120 177 5.44e-13 64.7
MsG0880042843.01.T01 MTR_4g120270 52.174 46 22 0 34 79 104 149 6.67e-13 63.9
MsG0880042843.01.T01 MTR_4g022410 40.000 70 38 1 18 87 101 166 7.22e-13 62.8
MsG0880042843.01.T01 MTR_8g092930 55.814 43 19 0 34 76 139 181 1.04e-12 62.8
MsG0880042843.01.T01 MTR_7g116180 44.444 54 27 1 24 77 89 139 1.04e-12 62.0
MsG0880042843.01.T01 MTR_8g023400 50.000 50 25 0 34 83 117 166 1.11e-12 63.5
MsG0880042843.01.T01 MTR_2g044570 51.020 49 24 0 31 79 80 128 1.22e-12 63.2
MsG0880042843.01.T01 MTR_8g092960 55.814 43 19 0 34 76 130 172 1.25e-12 62.4
MsG0880042843.01.T01 MTR_8g092840 55.814 43 19 0 34 76 140 182 1.49e-12 62.4
MsG0880042843.01.T01 MTR_8g092985 55.814 43 19 0 34 76 138 180 1.52e-12 62.0
MsG0880042843.01.T01 MTR_1g070425 44.444 54 27 1 24 77 90 140 1.55e-12 61.6
MsG0880042843.01.T01 MTR_4g106680 46.000 50 27 0 34 83 132 181 3.05e-12 62.4
MsG0880042843.01.T01 MTR_8g092990 53.488 43 20 0 34 76 141 183 3.07e-12 61.6
MsG0880042843.01.T01 MTR_8g092970 53.488 43 20 0 34 76 131 173 3.57e-12 61.2
MsG0880042843.01.T01 MTR_5g009210 47.619 63 29 1 15 77 300 358 4.66e-12 62.0
MsG0880042843.01.T01 MTR_3g071300 48.000 50 25 1 30 79 103 151 4.81e-12 60.1
MsG0880042843.01.T01 MTR_5g030010 40.000 80 40 2 2 79 120 193 6.06e-12 61.6
MsG0880042843.01.T01 MTR_7g079290 32.394 71 48 0 7 77 128 198 1.25e-11 59.7
MsG0880042843.01.T01 MTR_5g009200 54.545 44 20 0 34 77 350 393 1.71e-11 60.5
MsG0880042843.01.T01 MTR_3g087900 41.096 73 35 3 34 99 69 140 1.81e-11 58.2
MsG0880042843.01.T01 MTR_4g064550 45.902 61 30 2 27 85 208 267 1.81e-11 60.1
MsG0880042843.01.T01 MTR_4g063070 45.283 53 28 1 25 76 326 378 2.00e-11 60.1
MsG0880042843.01.T01 MTR_4g129420 40.741 54 32 0 34 87 144 197 2.10e-11 60.1
MsG0880042843.01.T01 MTR_8g009190 50.000 46 23 0 34 79 105 150 2.12e-11 60.1
MsG0880042843.01.T01 MTR_8g099495 41.071 56 33 0 21 76 334 389 2.12e-11 60.1
MsG0880042843.01.T01 MTR_5g064990 32.857 70 47 0 34 103 143 212 2.20e-11 60.1
MsG0880042843.01.T01 MTR_7g116220 28.125 96 59 2 1 87 67 161 2.35e-11 58.5
MsG0880042843.01.T01 MTR_5g009190 45.283 53 29 0 25 77 285 337 2.38e-11 60.1
MsG0880042843.01.T01 MTR_2g436100 42.593 54 31 0 34 87 119 172 2.81e-11 59.3
MsG0880042843.01.T01 MTR_3g078470 41.071 56 33 0 22 77 92 147 2.88e-11 58.9
MsG0880042843.01.T01 MTR_3g094000 45.652 46 25 0 34 79 119 164 3.03e-11 58.2
MsG0880042843.01.T01 MTR_1g056350 48.936 47 24 0 31 77 100 146 3.30e-11 57.8
MsG0880042843.01.T01 MTR_8g099515 39.062 64 39 0 21 84 324 387 3.35e-11 59.7
MsG0880042843.01.T01 MTR_1g100737 51.020 49 23 1 34 82 123 170 3.35e-11 58.2
MsG0880042843.01.T01 MTR_5g092790 34.783 69 45 0 17 85 168 236 3.59e-11 58.9
MsG0880042843.01.T01 MTR_5g092750 40.741 54 32 0 32 85 183 236 3.85e-11 58.5
MsG0880042843.01.T01 MTR_3g114500 42.308 52 30 0 34 85 71 122 4.20e-11 56.6
MsG0880042843.01.T01 MTR_2g035800 47.826 46 24 0 34 79 102 147 4.44e-11 57.4
MsG0880042843.01.T01 MTR_3g091620 52.273 44 21 0 34 77 127 170 4.68e-11 58.9
MsG0880042843.01.T01 MTR_7g096690 48.000 50 26 0 34 83 100 149 4.86e-11 58.9
MsG0880042843.01.T01 MTR_6g007737 45.652 46 25 0 34 79 159 204 5.03e-11 58.9
MsG0880042843.01.T01 MTR_3g086700 44.681 47 26 0 34 80 75 121 5.75e-11 57.4
MsG0880042843.01.T01 MTR_5g064890 40.000 65 33 1 15 79 141 199 6.85e-11 58.5
MsG0880042843.01.T01 MTR_4g064560 44.444 54 29 1 27 80 202 254 7.02e-11 58.2
MsG0880042843.01.T01 MTR_3g078450 47.727 44 23 0 34 77 104 147 7.09e-11 57.8
MsG0880042843.01.T01 MTR_3g078520 47.059 51 26 1 34 83 103 153 8.81e-11 56.6
MsG0880042843.01.T01 MTR_8g027745 41.071 56 33 0 21 76 301 356 9.14e-11 58.2
MsG0880042843.01.T01 MTR_5g026720 38.095 63 37 1 18 80 79 139 9.34e-11 56.6
MsG0880042843.01.T01 MTR_2g096350 45.652 46 25 0 34 79 135 180 9.93e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042843.01.T01 AT1G53820 38.554 83 30 1 34 95 120 202 1.25e-14 68.9
MsG0880042843.01.T01 AT4G40070 43.478 69 38 1 22 89 111 179 2.75e-14 68.2
MsG0880042843.01.T01 AT3G14320 50.000 46 23 0 34 79 88 133 6.45e-14 65.9
MsG0880042843.01.T01 AT5G43420 53.191 47 22 0 34 80 138 184 1.35e-13 66.2
MsG0880042843.01.T01 AT1G72310 47.826 46 24 0 34 79 127 172 1.46e-13 66.2
MsG0880042843.01.T01 AT5G06490 38.961 77 44 1 4 77 96 172 1.50e-13 64.7
MsG0880042843.01.T01 AT3G18930 39.683 63 38 0 17 79 139 201 2.46e-13 65.9
MsG0880042843.01.T01 AT3G18930 39.683 63 38 0 17 79 139 201 2.46e-13 65.9
MsG0880042843.01.T01 AT3G18930 39.683 63 38 0 17 79 139 201 2.46e-13 65.9
MsG0880042843.01.T01 AT1G53010 43.421 76 41 1 6 79 100 175 5.16e-13 62.8
MsG0880042843.01.T01 AT3G03550 46.269 67 30 1 13 79 144 204 9.15e-13 63.9
MsG0880042843.01.T01 AT1G04360 43.478 69 38 1 13 80 112 180 1.68e-12 63.2
MsG0880042843.01.T01 AT2G17450 44.000 50 28 0 27 76 95 144 2.35e-12 61.2
MsG0880042843.01.T01 AT5G17600 40.278 72 37 1 13 84 127 192 4.39e-12 62.0
MsG0880042843.01.T01 AT3G60220 54.348 46 21 0 34 79 117 162 4.72e-12 62.0
MsG0880042843.01.T01 AT5G05280 39.655 58 35 0 34 91 113 170 7.47e-12 59.7
MsG0880042843.01.T01 AT2G27940 54.545 44 20 0 34 77 140 183 8.16e-12 60.5
MsG0880042843.01.T01 AT3G55530 45.070 71 34 3 22 90 197 264 9.27e-12 60.8
MsG0880042843.01.T01 AT3G48030 45.833 48 26 0 34 81 129 176 1.20e-11 60.5
MsG0880042843.01.T01 AT5G40250 45.652 46 25 0 34 79 143 188 1.25e-11 60.8
MsG0880042843.01.T01 AT1G80400 40.984 61 35 1 24 84 345 404 1.25e-11 60.8
MsG0880042843.01.T01 AT3G48030 45.833 48 26 0 34 81 207 254 1.53e-11 60.5
MsG0880042843.01.T01 AT4G17905 50.000 46 23 0 34 79 181 226 1.72e-11 60.5
MsG0880042843.01.T01 AT3G05200 34.375 64 41 1 19 82 114 176 1.73e-11 60.5
MsG0880042843.01.T01 AT4G17905 50.000 46 23 0 34 79 155 200 1.86e-11 60.1
MsG0880042843.01.T01 AT3G60966 45.455 55 30 0 25 79 53 107 1.86e-11 58.2
MsG0880042843.01.T01 AT5G01880 45.652 46 25 0 34 79 105 150 2.37e-11 58.2
MsG0880042843.01.T01 AT4G28890 48.936 47 24 0 34 80 123 169 2.69e-11 60.1
MsG0880042843.01.T01 AT5G57750 31.818 88 44 1 34 105 122 209 3.15e-11 58.5
MsG0880042843.01.T01 AT3G16720 50.000 46 23 0 34 79 119 164 3.48e-11 59.3
MsG0880042843.01.T01 AT3G61550 34.722 72 41 1 5 76 113 178 4.08e-11 58.5
MsG0880042843.01.T01 AT1G72200 45.652 46 25 0 34 79 144 189 4.45e-11 59.3
MsG0880042843.01.T01 AT3G02340 44.828 58 30 2 22 79 324 379 5.12e-11 58.9
MsG0880042843.01.T01 AT3G02340 44.828 58 30 2 22 79 324 379 5.12e-11 58.9
MsG0880042843.01.T01 AT1G23980 47.826 46 24 0 34 79 144 189 5.38e-11 58.9
MsG0880042843.01.T01 AT1G32361 31.250 96 59 3 10 105 133 221 5.40e-11 58.9
MsG0880042843.01.T01 AT1G33480 40.323 62 37 0 34 95 100 161 5.49e-11 58.5
MsG0880042843.01.T01 AT2G47560 45.652 46 25 0 34 79 108 153 5.63e-11 58.2
MsG0880042843.01.T01 AT4G15975 50.000 46 23 0 34 79 76 121 6.85e-11 58.2
MsG0880042843.01.T01 AT2G44578 41.509 53 31 0 27 79 63 115 7.80e-11 56.6
MsG0880042843.01.T01 AT4G32600 34.328 67 43 1 29 95 358 423 8.76e-11 58.5
MsG0880042843.01.T01 AT4G32600 34.328 67 43 1 29 95 358 423 8.76e-11 58.5
MsG0880042843.01.T01 AT4G33565 43.548 62 29 1 18 79 207 262 9.53e-11 58.2

Find 16 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGATTCTGATTATTACTA+TGG 0.243856 8:+14775769 None:intergenic
GAAATCCTTTGAAGAATCTT+TGG 0.271628 8:-14775736 MsG0880042843.01.T01:CDS
GCAGAGTTTATGCAGAATCT+TGG 0.386567 8:+14775983 None:intergenic
ATCCTACATGTCCTCTTTGT+AGG 0.420010 8:-14775806 MsG0880042843.01.T01:CDS
ATGCTGATTGTATTGGTGCT+TGG 0.457961 8:-14775839 MsG0880042843.01.T01:CDS
CATAAACTCTGCAAGAATGA+TGG 0.493226 8:-14775972 MsG0880042843.01.T01:CDS
TGATATCTATGATTACATAA+AGG 0.505551 8:+14775861 None:intergenic
AAAGTCTCAGTGATACTAGA+TGG 0.508743 8:+14775657 None:intergenic
AGATATCATGCTGATTGTAT+TGG 0.513302 8:-14775846 MsG0880042843.01.T01:CDS
TGTTTAAGCCAAATGTGTAA+AGG 0.521394 8:-14775903 MsG0880042843.01.T01:CDS
CTCCTACAAAGAGGACATGT+AGG 0.557247 8:+14775804 None:intergenic
GTCAACCAAAGATTCTTCAA+AGG 0.564629 8:+14775731 None:intergenic
CATCAGAGAAACTTTGTACA+AGG 0.580071 8:+14775709 None:intergenic
TCAGTGAAGGAAATAGATCA+AGG 0.588335 8:-14775948 MsG0880042843.01.T01:CDS
CAAGAATGATGGTTCAGTGA+AGG 0.600245 8:-14775961 MsG0880042843.01.T01:CDS
AAAAGCATCAATAAACTACA+GGG 0.688994 8:+14775634 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATATCTATGATTACATAA+AGG + Chr8:14775794-14775813 None:intergenic 20.0%
! TAATCAGAATCAACAAAAAC+AGG - Chr8:14775892-14775911 MsG0880042843.01.T01:CDS 25.0%
!! GTTGATTCTGATTATTACTA+TGG + Chr8:14775886-14775905 None:intergenic 25.0%
GTGTAAAGGAGAAAAAGTTA+AGG - Chr8:14775763-14775782 MsG0880042843.01.T01:CDS 30.0%
TGTTTAAGCCAAATGTGTAA+AGG - Chr8:14775749-14775768 MsG0880042843.01.T01:CDS 30.0%
! AGATATCATGCTGATTGTAT+TGG - Chr8:14775806-14775825 MsG0880042843.01.T01:CDS 30.0%
! CTTTTTCTCCTTTACACATT+TGG + Chr8:14775760-14775779 None:intergenic 30.0%
! GAAATCCTTTGAAGAATCTT+TGG - Chr8:14775916-14775935 MsG0880042843.01.T01:CDS 30.0%
AAAGTCTCAGTGATACTAGA+TGG + Chr8:14775998-14776017 None:intergenic 35.0%
CATAAACTCTGCAAGAATGA+TGG - Chr8:14775680-14775699 MsG0880042843.01.T01:CDS 35.0%
CATCAGAGAAACTTTGTACA+AGG + Chr8:14775946-14775965 None:intergenic 35.0%
GTCAACCAAAGATTCTTCAA+AGG + Chr8:14775924-14775943 None:intergenic 35.0%
TCAGTGAAGGAAATAGATCA+AGG - Chr8:14775704-14775723 MsG0880042843.01.T01:CDS 35.0%
ATCCTACATGTCCTCTTTGT+AGG - Chr8:14775846-14775865 MsG0880042843.01.T01:CDS 40.0%
CAAGAATGATGGTTCAGTGA+AGG - Chr8:14775691-14775710 MsG0880042843.01.T01:CDS 40.0%
GCAGAGTTTATGCAGAATCT+TGG + Chr8:14775672-14775691 None:intergenic 40.0%
! ATGCTGATTGTATTGGTGCT+TGG - Chr8:14775813-14775832 MsG0880042843.01.T01:CDS 40.0%
! CTGATTTTGCTCCTACAAAG+AGG + Chr8:14775860-14775879 None:intergenic 40.0%
CTCCTACAAAGAGGACATGT+AGG + Chr8:14775851-14775870 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
Chr8 gene 14775641 14776033 14775641 ID=MsG0880042843.01;Name=MsG0880042843.01
Chr8 mRNA 14775641 14776033 14775641 ID=MsG0880042843.01.T01;Parent=MsG0880042843.01;Name=MsG0880042843.01.T01;_AED=0.31;_eAED=0.31;_QI=0|-1|0|1|-1|1|1|0|130
Chr8 exon 14775641 14776033 14775641 ID=MsG0880042843.01.T01:exon:36974;Parent=MsG0880042843.01.T01
Chr8 CDS 14775641 14776033 14775641 ID=MsG0880042843.01.T01:cds;Parent=MsG0880042843.01.T01
Gene Sequence

>MsG0880042843.01.T01

ATGGCTAACTCATTTCAAGAAAACATAACCAAGATTCTGCATAAACTCTGCAAGAATGATGGTTCAGTGAAGGAAATAGATCAAGGTGAAAGTGATTGTTGTTCAGTTTGTTTAAGCCAAATGTGTAAAGGAGAAAAAGTTAAGGTATTACCTTTATGTAATCATAGATATCATGCTGATTGTATTGGTGCTTGGTTGAAGAACAATCCTACATGTCCTCTTTGTAGGAGCAAAATCAGTAACCATAGTAATAATCAGAATCAACAAAAACAGGTGAAATCCTTTGAAGAATCTTTGGTTGACCTTGTACAAAGTTTCTCTGATGTACTTGTAGCTATTCTTTATATGATTCTGCCATCTAGTATCACTGAGACTTTCCCTGTAGTTTATTGA

Protein sequence

>MsG0880042843.01.T01

MANSFQENITKILHKLCKNDGSVKEIDQGESDCCSVCLSQMCKGEKVKVLPLCNHRYHADCIGAWLKNNPTCPLCRSKISNHSNNQNQQKQVKSFEESLVDLVQSFSDVLVAILYMILPSSITETFPVVY*