AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000567.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000567.01.T01 MTR_1g013130 85.171 263 5 2 1 230 1 262 4.48e-162 448
MsG0180000567.01.T01 MTR_3g106230 41.102 236 82 9 23 215 4 225 2.11e-38 133
MsG0180000567.01.T01 MTR_2g104900 28.521 284 112 13 1 230 1 247 1.11e-13 68.6
MsG0180000567.01.T01 MTR_5g077370 36.975 119 48 4 60 178 89 180 2.47e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000567.01.T01 AT5G10970 39.216 204 78 9 49 230 93 272 3.97e-27 105
MsG0180000567.01.T01 AT5G25160 40.351 171 65 8 34 189 20 168 3.23e-23 94.0
MsG0180000567.01.T01 AT1G80730 36.723 177 90 7 60 230 68 228 3.38e-18 80.5

Find 30 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCAAACTCAAGAATCTTTA+TGG 0.122503 1:+7961303 MsG0180000567.01.T01:CDS
ATGGCTTCTTTGCCTCTTTA+TGG 0.168485 1:+7961163 MsG0180000567.01.T01:CDS
CTTTGTTTATTATGCATAAT+AGG 0.201649 1:-7961130 None:intergenic
CCTTCTCATGTTTCATCTAA+TGG 0.202335 1:+7961241 MsG0180000567.01.T01:CDS
CATGTTTCATCTAATGGATT+TGG 0.228057 1:+7961247 MsG0180000567.01.T01:CDS
AATGGATTTGGAAACTCTTA+TGG 0.229174 1:+7961259 MsG0180000567.01.T01:CDS
ATAGCTTCAGCTTCTGCTTT+TGG 0.233602 1:+7961103 MsG0180000567.01.T01:CDS
TTGCCTCTTTATGGTGCTTT+TGG 0.261823 1:+7961172 MsG0180000567.01.T01:CDS
ATGAAACATGAGAAGGCTTA+TGG 0.351379 1:-7961234 None:intergenic
CTCATAACTTCTTTAGATGT+TGG 0.385754 1:+7960912 MsG0180000567.01.T01:CDS
TGCAAAGGTGTGCTTGGACT+TGG 0.428789 1:-7960750 None:intergenic
TTTGGTGGTAATAAACCTCT+TGG 0.453972 1:+7961190 MsG0180000567.01.T01:CDS
AAAGGTGTGCTTGGACTTGG+AGG 0.516654 1:-7960747 None:intergenic
TGGAATAGCAAAACTAGGTA+TGG 0.517898 1:+7961336 MsG0180000567.01.T01:CDS
ATCTTTATGGATCAACAACC+TGG 0.518646 1:+7961316 MsG0180000567.01.T01:CDS
CAACCTGGAATAGCAAAACT+AGG 0.529841 1:+7961331 MsG0180000567.01.T01:CDS
CCACCAAAAGCACCATAAAG+AGG 0.536698 1:-7961175 None:intergenic
CATCAAGAGGAAGTGTTGGT+AGG 0.548091 1:+7961383 MsG0180000567.01.T01:CDS
AAGTGTTGGTAGGTTTGAAG+TGG 0.552808 1:+7961393 MsG0180000567.01.T01:CDS
GAAGAAACAACAATCTATTG+AGG 0.558110 1:+7960788 MsG0180000567.01.T01:CDS
ATTAATGGAAAGACTAAGCA+AGG 0.563627 1:+7960695 None:intergenic
TATGGACATCATCATCATCA+TGG 0.564611 1:+7961277 MsG0180000567.01.T01:CDS
TCTTGAGAAGAGAAGATGCA+TGG 0.579539 1:-7960720 None:intergenic
GGTAGAAGAAGATATCACTA+AGG 0.583712 1:+7960809 MsG0180000567.01.T01:CDS
ACATCATCAAGAGGAAGTGT+TGG 0.585725 1:+7961379 MsG0180000567.01.T01:CDS
AGAACAACAACATCATCAAG+AGG 0.611847 1:+7961370 MsG0180000567.01.T01:CDS
ATATTCATAATCATAGCTTG+AGG 0.612882 1:-7961477 None:intergenic
TAAACAAAGTTATGCAAACA+TGG 0.616969 1:+7961144 MsG0180000567.01.T01:CDS
CCATTAGATGAAACATGAGA+AGG 0.626125 1:-7961241 None:intergenic
GAGTGTGCTTGAATTCCAAG+AGG 0.647089 1:-7961205 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTGTTTATTATGCATAAT+AGG - Chr1:7961133-7961152 None:intergenic 20.0%
! TAAACAAAGTTATGCAAACA+TGG + Chr1:7961144-7961163 MsG0180000567.01.T01:CDS 25.0%
!!! TTTTCATGTTGATTTTGCAA+AGG - Chr1:7960768-7960787 None:intergenic 25.0%
CTCATAACTTCTTTAGATGT+TGG + Chr1:7960912-7960931 MsG0180000567.01.T01:CDS 30.0%
GAAGAAACAACAATCTATTG+AGG + Chr1:7960788-7960807 MsG0180000567.01.T01:CDS 30.0%
GTCAAACTCAAGAATCTTTA+TGG + Chr1:7961303-7961322 MsG0180000567.01.T01:CDS 30.0%
! AATGGATTTGGAAACTCTTA+TGG + Chr1:7961259-7961278 MsG0180000567.01.T01:CDS 30.0%
!! CATGTTTCATCTAATGGATT+TGG + Chr1:7961247-7961266 MsG0180000567.01.T01:CDS 30.0%
AGAACAACAACATCATCAAG+AGG + Chr1:7961370-7961389 MsG0180000567.01.T01:CDS 35.0%
ATCTTTATGGATCAACAACC+TGG + Chr1:7961316-7961335 MsG0180000567.01.T01:CDS 35.0%
ATGAAACATGAGAAGGCTTA+TGG - Chr1:7961237-7961256 None:intergenic 35.0%
CCATTAGATGAAACATGAGA+AGG - Chr1:7961244-7961263 None:intergenic 35.0%
CCTTCTCATGTTTCATCTAA+TGG + Chr1:7961241-7961260 MsG0180000567.01.T01:CDS 35.0%
GAGAGATCAATAGCAAAAAG+AGG + Chr1:7961058-7961077 MsG0180000567.01.T01:CDS 35.0%
GCAAAAAGAGGACAAAGATT+TGG + Chr1:7961070-7961089 MsG0180000567.01.T01:CDS 35.0%
GGTAGAAGAAGATATCACTA+AGG + Chr1:7960809-7960828 MsG0180000567.01.T01:CDS 35.0%
TATGGACATCATCATCATCA+TGG + Chr1:7961277-7961296 MsG0180000567.01.T01:CDS 35.0%
TGGAATAGCAAAACTAGGTA+TGG + Chr1:7961336-7961355 MsG0180000567.01.T01:CDS 35.0%
TTCTATAGCTCACAAGCATT+AGG + Chr1:7961010-7961029 MsG0180000567.01.T01:intron 35.0%
! TCTCTCTTTTGTGAGCATTT+TGG - Chr1:7961042-7961061 None:intergenic 35.0%
! TTTGGTGGTAATAAACCTCT+TGG + Chr1:7961190-7961209 MsG0180000567.01.T01:CDS 35.0%
!!! ATACCTAGTTTTGCTATTCC+AGG - Chr1:7961337-7961356 None:intergenic 35.0%
ATGGCTTCTTTGCCTCTTTA+TGG + Chr1:7961163-7961182 MsG0180000567.01.T01:CDS 40.0%
CAACCTGGAATAGCAAAACT+AGG + Chr1:7961331-7961350 MsG0180000567.01.T01:CDS 40.0%
GACATCATCATCATCATGGT+TGG + Chr1:7961281-7961300 MsG0180000567.01.T01:CDS 40.0%
TATAGCTCACAAGCATTAGG+TGG + Chr1:7961013-7961032 MsG0180000567.01.T01:intron 40.0%
TCTTGAGAAGAGAAGATGCA+TGG - Chr1:7960723-7960742 None:intergenic 40.0%
! ACATCATCAAGAGGAAGTGT+TGG + Chr1:7961379-7961398 MsG0180000567.01.T01:CDS 40.0%
! ATAGCTTCAGCTTCTGCTTT+TGG + Chr1:7961103-7961122 MsG0180000567.01.T01:CDS 40.0%
! TTGCCTCTTTATGGTGCTTT+TGG + Chr1:7961172-7961191 MsG0180000567.01.T01:CDS 40.0%
!! AAGTGTTGGTAGGTTTGAAG+TGG + Chr1:7961393-7961412 MsG0180000567.01.T01:CDS 40.0%
!! TGATTTTGCAAAGGTGTGCT+TGG - Chr1:7960759-7960778 None:intergenic 40.0%
GAGTGTGCTTGAATTCCAAG+AGG - Chr1:7961208-7961227 None:intergenic 45.0%
! CCTCTTTATGGTGCTTTTGG+TGG + Chr1:7961175-7961194 MsG0180000567.01.T01:CDS 45.0%
!! CATCAAGAGGAAGTGTTGGT+AGG + Chr1:7961383-7961402 MsG0180000567.01.T01:CDS 45.0%
!! CCACCAAAAGCACCATAAAG+AGG - Chr1:7961178-7961197 None:intergenic 45.0%
! AAAGGTGTGCTTGGACTTGG+AGG - Chr1:7960750-7960769 None:intergenic 50.0%
! TGCAAAGGTGTGCTTGGACT+TGG - Chr1:7960753-7960772 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr1 gene 7960699 7961489 7960699 ID=MsG0180000567.01;Name=MsG0180000567.01
Chr1 mRNA 7960699 7961489 7960699 ID=MsG0180000567.01.T01;Parent=MsG0180000567.01;Name=MsG0180000567.01.T01;_AED=0.21;_eAED=0.22;_QI=0|0|0|1|1|1|2|0|230
Chr1 exon 7960699 7960933 7960699 ID=MsG0180000567.01.T01:exon:2715;Parent=MsG0180000567.01.T01
Chr1 exon 7961032 7961489 7961032 ID=MsG0180000567.01.T01:exon:2714;Parent=MsG0180000567.01.T01
Chr1 CDS 7960699 7960933 7960699 ID=MsG0180000567.01.T01:cds;Parent=MsG0180000567.01.T01
Chr1 CDS 7961032 7961489 7961032 ID=MsG0180000567.01.T01:cds;Parent=MsG0180000567.01.T01
Gene Sequence

>MsG0180000567.01.T01

ATGGAAAGACTAAGCAAGGAACCATGCATCTTCTCTTCTCAAGAAACTCCTCCAAGTCCAAGCACACCTTTGCAAAATCAACATGAAAAGAAGAAACAACAATCTATTGAGGTAGAAGAAGATATCACTAAGGTAGAAACTTTATTTGATCTAAATGTATCTAGCAATGATTCTAATCTTGATTGTAATAGTCAGCAAGAAACAGAACTCAATCTCATAACTTCTTTAGATGTTGGTGGACACCAAAATGCTCACAAAAGAGAGAGATCAATAGCAAAAAGAGGACAAAGATTTGGAACACAAATAATAGCTTCAGCTTCTGCTTTTGGATTTCCTATTATGCATAATAAACAAAGTTATGCAAACATGGCTTCTTTGCCTCTTTATGGTGCTTTTGGTGGTAATAAACCTCTTGGAATTCAAGCACACTCTATGATCCATAAGCCTTCTCATGTTTCATCTAATGGATTTGGAAACTCTTATGGACATCATCATCATCATGGTTGGTCAAACTCAAGAATCTTTATGGATCAACAACCTGGAATAGCAAAACTAGGTATGGCTGATTTTCATAGAACAACAACATCATCAAGAGGAAGTGTTGGTAGGTTTGAAGTGGTAGATACTATGATTAATTCTGCAGCTAATAAAGAAATGAAGCAGAAGCAGCTTGATTTAAACCTCAAGCTATGA

Protein sequence

>MsG0180000567.01.T01

MERLSKEPCIFSSQETPPSPSTPLQNQHEKKKQQSIEVEEDITKVETLFDLNVSSNDSNLDCNSQQETELNLITSLDVGGHQNAHKRERSIAKRGQRFGTQIIASASAFGFPIMHNKQSYANMASLPLYGAFGGNKPLGIQAHSMIHKPSHVSSNGFGNSYGHHHHHGWSNSRIFMDQQPGIAKLGMADFHRTTTSSRGSVGRFEVVDTMINSAANKEMKQKQLDLNLKL*