AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006439.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006439.01.T01 MTR_2g008430 98.778 409 5 0 6 414 32 440 0.0 840
MsG0280006439.01.T01 MTR_4g092620 65.764 406 139 0 7 412 52 457 0.0 553
MsG0280006439.01.T01 MTR_4g011820 29.080 337 213 14 82 410 64 382 4.53e-18 85.9
MsG0280006439.01.T01 MTR_7g106830 24.173 393 246 13 46 413 99 464 1.20e-17 85.1
MsG0280006439.01.T01 MTR_8g091280 23.919 393 247 14 46 413 101 466 2.55e-17 84.0
MsG0280006439.01.T01 MTR_3g072680 25.641 351 226 13 77 413 71 400 3.92e-13 70.9
MsG0280006439.01.T01 MTR_3g072670 24.079 353 233 13 77 415 114 445 6.35e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006439.01.T01 AT2G13810 69.363 408 125 0 3 410 29 436 0.0 608
MsG0280006439.01.T01 AT4G33680 64.593 418 141 1 2 412 42 459 0.0 553
MsG0280006439.01.T01 AT2G22250 23.590 390 252 14 46 413 54 419 5.71e-16 79.7
MsG0280006439.01.T01 AT2G22250 23.590 390 252 14 46 413 101 466 8.70e-16 79.3
MsG0280006439.01.T01 AT2G22250 23.590 390 252 14 46 413 101 466 8.70e-16 79.3
MsG0280006439.01.T01 AT1G80360 26.857 350 203 16 82 410 64 381 1.94e-15 78.2
MsG0280006439.01.T01 AT1G80360 26.857 350 203 16 82 410 64 381 1.94e-15 78.2
MsG0280006439.01.T01 AT1G80360 26.857 350 203 16 82 410 64 381 1.94e-15 78.2
MsG0280006439.01.T01 AT1G80360 26.857 350 203 16 82 410 64 381 1.94e-15 78.2
MsG0280006439.01.T01 AT1G77670 25.000 352 223 14 80 416 114 439 9.16e-13 70.1
MsG0280006439.01.T01 AT4G28420 28.139 231 151 7 59 282 74 296 1.62e-11 65.9
MsG0280006439.01.T01 AT4G28420 28.139 231 151 7 59 282 74 296 2.11e-11 65.9

Find 110 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTTGGTTTGACAGTTTA+TGG 0.110786 2:+1961508 MsG0280006439.01.T01:CDS
GGAATTTCATAGATTGATTT+AGG 0.187168 2:-1960946 None:intergenic
TATGTTTGGGTCCGCTTTCC+TGG 0.228427 2:+1961547 MsG0280006439.01.T01:CDS
ATTATGACTGTTAAATGTTT+AGG 0.278883 2:+1958532 None:intergenic
TTTGAATATAACAGTTCTTC+AGG 0.285273 2:-1961148 None:intergenic
TATATAAGAATTAGTGCTTT+TGG 0.288222 2:+1961661 MsG0280006439.01.T01:CDS
ACTATTCCAGGGAGTGGTTT+TGG 0.293363 2:+1961622 MsG0280006439.01.T01:CDS
TGTACAGGATCCATCCTTTC+CGG 0.296228 2:+1959512 MsG0280006439.01.T01:CDS
CAAACACATGCAGCTGCTAA+TGG 0.296722 2:+1959470 MsG0280006439.01.T01:intron
GCTTTCCTGGCTCAAAATCT+TGG 0.306405 2:+1961560 MsG0280006439.01.T01:CDS
CATAAAGTTTACAAAGATCT+AGG 0.316232 2:+1959301 MsG0280006439.01.T01:CDS
ATGTGATATTTCTCGTCTTC+AGG 0.327356 2:+1959363 MsG0280006439.01.T01:CDS
GCAGAAGGTTATAAAGGATA+TGG 0.331582 2:+1959101 MsG0280006439.01.T01:CDS
TCAACAGCAGAAGGTTATAA+AGG 0.340368 2:+1959095 MsG0280006439.01.T01:CDS
GTCACTGCTTTGACCTCTCT+TGG 0.347267 2:+1961493 MsG0280006439.01.T01:CDS
CGGTTGAAATCATGCAAAAC+AGG 0.348940 2:-1961178 None:intergenic
TCGAAATTTGCCGGCTTCAC+AGG 0.351995 2:+1961100 MsG0280006439.01.T01:CDS
AAATCAATCTATGAAATTCC+TGG 0.359372 2:+1960949 MsG0280006439.01.T01:CDS
CTTGAAGTGGTATGCAGATC+CGG 0.370419 2:-1960772 None:intergenic
TCATCCTTCTCGAAATTTGC+CGG 0.372764 2:+1961091 MsG0280006439.01.T01:CDS
ATCCGGGAAGAAGTCAGTTT+GGG 0.373320 2:-1960755 None:intergenic
GCCCCAAACTGACTTCTTCC+CGG 0.390448 2:+1960753 MsG0280006439.01.T01:CDS
ATAGACTCAAGTGTCATAAT+TGG 0.398736 2:+1960667 MsG0280006439.01.T01:CDS
GATCCGGGAAGAAGTCAGTT+TGG 0.399877 2:-1960756 None:intergenic
ATGCTTGATGTTACAATTGT+TGG 0.402020 2:-1958859 None:intergenic
CTTTCCTGGCTCAAAATCTT+GGG 0.407856 2:+1961561 MsG0280006439.01.T01:CDS
GCATGGCCAGTAGGATTGTT+TGG 0.408006 2:-1960820 None:intergenic
AATGATTGATCCATTAACTT+TGG 0.413023 2:-1960881 None:intergenic
GGCAAGTATGTAGATAGAAT+TGG 0.420501 2:+1960697 MsG0280006439.01.T01:CDS
GGCTTCACAGGTGTTCGCCT+TGG 0.425448 2:+1961112 MsG0280006439.01.T01:CDS
AACCTTACATTAAGACCCTC+TGG 0.433061 2:-1961268 None:intergenic
AAACACATGCAGCTGCTAAT+GGG 0.442772 2:+1959471 MsG0280006439.01.T01:intron
ACACACATAATTACTATTCC+AGG 0.449699 2:+1961610 MsG0280006439.01.T01:CDS
TGATGTTACAATTGTTGGTA+AGG 0.452147 2:-1958854 None:intergenic
CGGATCTGCATACCACTTCA+AGG 0.457675 2:+1960773 MsG0280006439.01.T01:CDS
GAAGAACTGTTATATTCAAA+CGG 0.460687 2:+1961151 MsG0280006439.01.T01:CDS
AATGTTATAGACCTTGGAAT+TGG 0.461601 2:+1958817 MsG0280006439.01.T01:CDS
CTTATATACCCTTCACCACC+TGG 0.471155 2:-1961646 None:intergenic
CTTGTTGCTGCATGGCCAGT+AGG 0.473656 2:-1960829 None:intergenic
TCACAGGTGTTCGCCTTGGT+TGG 0.479516 2:+1961116 MsG0280006439.01.T01:CDS
AAACATACTTCTTTGGCTCC+AGG 0.484217 2:-1960967 None:intergenic
TGTACAGATATCTTCAGATT+GGG 0.491827 2:-1959495 None:intergenic
TTGAATATAACAGTTCTTCA+GGG 0.492697 2:-1961147 None:intergenic
CATGGAGAATGCAAGAATAC+TGG 0.496202 2:+1961471 MsG0280006439.01.T01:CDS
AAGGCGAACACCTGTGAAGC+CGG 0.499134 2:-1961110 None:intergenic
CATGCAAATGTTATAGACCT+TGG 0.500055 2:+1958811 MsG0280006439.01.T01:CDS
TGGACCTGAGCAAGGTGAAA+AGG 0.500308 2:+1959121 MsG0280006439.01.T01:CDS
GGAAAGCGGACCCAAACATA+TGG 0.501377 2:-1961544 None:intergenic
TTCTCCATGTTTACATTCCG+AGG 0.501871 2:-1958571 None:intergenic
CTGTACAGATATCTTCAGAT+TGG 0.506141 2:-1959496 None:intergenic
AAAACAATTGCAGCAACTTG+TGG 0.508111 2:+1960852 MsG0280006439.01.T01:CDS
GCATCCAACATATCTCAGGC+TGG 0.509357 2:+1961226 MsG0280006439.01.T01:CDS
CTTCAATGATAGAATCTCTC+TGG 0.509706 2:-1961684 None:intergenic
CAATCTGAAGATATCTGTAC+AGG 0.514291 2:+1959497 MsG0280006439.01.T01:CDS
ATAGTGTGTACCTGCTTCAA+TGG 0.516829 2:+1961202 MsG0280006439.01.T01:CDS
TGGAGCATCCAACATATCTC+AGG 0.525531 2:+1961222 MsG0280006439.01.T01:CDS
ATTGACAATACCGGAAAGGA+TGG 0.528116 2:-1959522 None:intergenic
AGTGTCATAATTGGTCATGC+TGG 0.532398 2:+1960676 MsG0280006439.01.T01:CDS
CATCCAACATATCTCAGGCT+GGG 0.534398 2:+1961227 MsG0280006439.01.T01:CDS
ATCCAACATATCTCAGGCTG+GGG 0.540828 2:+1961228 MsG0280006439.01.T01:CDS
ATAATTACTATTCCAGGGAG+TGG 0.544140 2:+1961616 MsG0280006439.01.T01:CDS
TTGTAGTATCACCAATTCCA+AGG 0.544858 2:-1958828 None:intergenic
ACCTTACATTAAGACCCTCT+GGG 0.545435 2:-1961267 None:intergenic
GATGTTACAATTGTTGGTAA+GGG 0.546951 2:-1958853 None:intergenic
CCTGGACCAAAACCACTCCC+TGG 0.547794 2:-1961628 None:intergenic
TATGACACTTGAGTCTATGT+AGG 0.548507 2:-1960662 None:intergenic
ATAAAGTTTACAAAGATCTA+GGG 0.555347 2:+1959302 MsG0280006439.01.T01:CDS
CTTGGTTTGACAGTTTATGG+TGG 0.556690 2:+1961511 MsG0280006439.01.T01:CDS
CATGGTCTTTCAACAGCAGA+AGG 0.559322 2:+1959086 MsG0280006439.01.T01:CDS
TTGAAGTGGTATGCAGATCC+GGG 0.559418 2:-1960771 None:intergenic
TGTTCACCAAACAATCCTAC+TGG 0.560664 2:+1960814 MsG0280006439.01.T01:CDS
GTTGGATGCTCCATTGAAGC+AGG 0.560853 2:-1961212 None:intergenic
AACATAGAATACATGACATG+TGG 0.564642 2:+1960730 MsG0280006439.01.T01:CDS
GTCCCTTGTTGACTACTACA+TGG 0.567716 2:+1961453 MsG0280006439.01.T01:CDS
TATTCTGCTTATATAACTGA+TGG 0.570219 2:+1960919 MsG0280006439.01.T01:CDS
AATAAGTTGTGCCCTTGAAG+TGG 0.570896 2:-1960785 None:intergenic
CTAGCATGTCTTTCCCCAGA+GGG 0.582421 2:+1961253 MsG0280006439.01.T01:CDS
AACAATTGTAACATCAAGCA+TGG 0.584348 2:+1958861 MsG0280006439.01.T01:CDS
ACTCAAATACCTTCTCTACC+AGG 0.587704 2:+1961717 MsG0280006439.01.T01:CDS
ACATGGCTATTTATTTCCTG+AGG 0.588374 2:+1958600 MsG0280006439.01.T01:CDS
AGAATCCCTCCTGGTAGAGA+AGG 0.594512 2:-1961726 None:intergenic
GGATCTGCATACCACTTCAA+GGG 0.613569 2:+1960774 MsG0280006439.01.T01:CDS
GAATATTGACAATACCGGAA+AGG 0.613754 2:-1959526 None:intergenic
CACACATAATTACTATTCCA+GGG 0.618107 2:+1961611 MsG0280006439.01.T01:CDS
TAAAACGTTAGAATCCCTCC+TGG 0.618520 2:-1961735 None:intergenic
AAAGGATATGGACCTGAGCA+AGG 0.621235 2:+1959113 MsG0280006439.01.T01:CDS
TGTTCGCCTTGGTTGGACAG+TGG 0.630562 2:+1961123 MsG0280006439.01.T01:CDS
GACACAGCACAAAGGTACCT+CGG 0.632754 2:+1958554 MsG0280006439.01.T01:CDS
GGTACCTCGGAATGTAAACA+TGG 0.633076 2:+1958567 MsG0280006439.01.T01:CDS
CAACTTGTAGGCTCTGAGGA+AGG 0.633117 2:+1959270 MsG0280006439.01.T01:intron
ACATAGAATACATGACATGT+GGG 0.634289 2:+1960731 MsG0280006439.01.T01:CDS
TGAACAACTTGTAGGCTCTG+AGG 0.635522 2:+1959266 MsG0280006439.01.T01:intron
TCTCCATGTAGTAGTCAACA+AGG 0.644639 2:-1961456 None:intergenic
GGTTGAAATCATGCAAAACA+GGG 0.656554 2:-1961177 None:intergenic
ATAAACTGTCAAACCAAGAG+AGG 0.659137 2:-1961506 None:intergenic
TCTAGGGATAAAACCCTCAG+AGG 0.665983 2:+1959318 MsG0280006439.01.T01:CDS
ACTAGCATGTCTTTCCCCAG+AGG 0.667392 2:+1961252 MsG0280006439.01.T01:CDS
TCCGGGAAGAAGTCAGTTTG+GGG 0.669817 2:-1960754 None:intergenic
AAATACCTTCTCTACCAGGA+GGG 0.670279 2:+1961721 MsG0280006439.01.T01:CDS
AGGTCTATAACATTTGCATG+TGG 0.672607 2:-1958808 None:intergenic
CAGGGACCACTGTCCAACCA+AGG 0.676075 2:-1961129 None:intergenic
GAAGCCGGCAAATTTCGAGA+AGG 0.677853 2:-1961095 None:intergenic
AAGGTATTTGAGTCTCTCTG+AGG 0.680422 2:-1961707 None:intergenic
TCCAACATATCTCAGGCTGG+GGG 0.684780 2:+1961229 MsG0280006439.01.T01:CDS
CTCCATGTAGTAGTCAACAA+GGG 0.687840 2:-1961455 None:intergenic
CAAATACCTTCTCTACCAGG+AGG 0.694255 2:+1961720 MsG0280006439.01.T01:CDS
AACATGGAGAAACTTCAACA+TGG 0.713385 2:+1958583 MsG0280006439.01.T01:CDS
ATGTTTAGGACACAGCACAA+AGG 0.717428 2:+1958546 MsG0280006439.01.T01:CDS
TGAAGCAGGTACACACTATG+CGG 0.736249 2:-1961198 None:intergenic
CCTTACATTAAGACCCTCTG+GGG 0.756474 2:-1961266 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAGGTTAAATATAATTT+TGG - Chr2:1960156-1960175 None:intergenic 10.0%
!! CCAAAAAAAAAAACAATAAA+TGG + Chr2:1960099-1960118 MsG0280006439.01.T01:intron 15.0%
!! TATAATAATGTATTGTTTCA+AGG + Chr2:1960574-1960593 MsG0280006439.01.T01:intron 15.0%
!!! AAATTTTCCAAAACATTATT+TGG - Chr2:1961420-1961439 None:intergenic 15.0%
!!! CCATTTATTGTTTTTTTTTT+TGG - Chr2:1960102-1960121 None:intergenic 15.0%
!!! TATATACTACTTTTTGATTT+TGG + Chr2:1959915-1959934 MsG0280006439.01.T01:intron 15.0%
!! ATAAAGTTTACAAAGATCTA+GGG + Chr2:1959302-1959321 MsG0280006439.01.T01:CDS 20.0%
!! ATACAATTGAAAATGCAAAT+AGG - Chr2:1960274-1960293 None:intergenic 20.0%
!! CATGCAGTTAATAAATAAAA+AGG + Chr2:1959671-1959690 MsG0280006439.01.T01:intron 20.0%
!! TCAACTAGATCATTTATTAA+GGG + Chr2:1960541-1960560 MsG0280006439.01.T01:intron 20.0%
!! TGTACATTAGTAGAAAAATA+TGG + Chr2:1960487-1960506 MsG0280006439.01.T01:intron 20.0%
!! TTATTGAATATTGACAATAC+CGG - Chr2:1959534-1959553 None:intergenic 20.0%
!! TTCAACTAGATCATTTATTA+AGG + Chr2:1960540-1960559 MsG0280006439.01.T01:intron 20.0%
!!! AAAATGGAATACAGTATTTT+AGG - Chr2:1960172-1960191 None:intergenic 20.0%
!!! AAATTTGTTCATGTTGAAAT+TGG + Chr2:1959964-1959983 MsG0280006439.01.T01:intron 20.0%
!!! CTTTTTATTTATTAACTGCA+TGG - Chr2:1959673-1959692 None:intergenic 20.0%
!!! TATATAAGAATTAGTGCTTT+TGG + Chr2:1961661-1961680 MsG0280006439.01.T01:CDS 20.0%
!!! TTTTTCCTTTATTTCTGTTA+TGG + Chr2:1958938-1958957 MsG0280006439.01.T01:intron 20.0%
! AAATATGGTCAAAGTAAGTT+AGG + Chr2:1960502-1960521 MsG0280006439.01.T01:intron 25.0%
! AAATCAATCTATGAAATTCC+TGG + Chr2:1960949-1960968 MsG0280006439.01.T01:CDS 25.0%
! AAGAGGAAATATTCTTGATT+TGG + Chr2:1959030-1959049 MsG0280006439.01.T01:intron 25.0%
! AATAGGACAATATTATTGCT+TGG - Chr2:1960257-1960276 None:intergenic 25.0%
! AATGATTGATCCATTAACTT+TGG - Chr2:1960884-1960903 None:intergenic 25.0%
! ACAAGTTATACAGGTTATTA+GGG - Chr2:1961300-1961319 None:intergenic 25.0%
! ATCTTCCATAACAGAAATAA+AGG - Chr2:1958946-1958965 None:intergenic 25.0%
! CAAATTCAAATCATGGAAAA+TGG - Chr2:1960188-1960207 None:intergenic 25.0%
! CATAAAGTTTACAAAGATCT+AGG + Chr2:1959301-1959320 MsG0280006439.01.T01:CDS 25.0%
! CATCATACAATTTAGATAGA+TGG - Chr2:1959585-1959604 None:intergenic 25.0%
! CTAGAAGTAAAAAAAAAACG+TGG - Chr2:1959717-1959736 None:intergenic 25.0%
! GAAGAACTGTTATATTCAAA+CGG + Chr2:1961151-1961170 MsG0280006439.01.T01:CDS 25.0%
! TAAAAATGCACCATATGTTT+GGG + Chr2:1961534-1961553 MsG0280006439.01.T01:CDS 25.0%
! TAATGAAATTGTTACGTCAA+TGG + Chr2:1961383-1961402 MsG0280006439.01.T01:intron 25.0%
! TAGACGTTAATAAACTACTT+TGG + Chr2:1959615-1959634 MsG0280006439.01.T01:intron 25.0%
! TAGCTTATAGCAATATGAAT+TGG - Chr2:1958731-1958750 None:intergenic 25.0%
! TATTCTGCTTATATAACTGA+TGG + Chr2:1960919-1960938 MsG0280006439.01.T01:CDS 25.0%
! TTAACCATAAAGTTATGCAT+TGG - Chr2:1960366-1960385 None:intergenic 25.0%
! TTATGAAGAGTAAGTAGTAT+TGG - Chr2:1959400-1959419 None:intergenic 25.0%
! TTGAATATAACAGTTCTTCA+GGG - Chr2:1961150-1961169 None:intergenic 25.0%
! TTGTAAGCAAATTCAAATCA+TGG - Chr2:1960195-1960214 None:intergenic 25.0%
! TTTGAATATAACAGTTCTTC+AGG - Chr2:1961151-1961170 None:intergenic 25.0%
!! ATGGTTGATGTAAGTTATAA+AGG + Chr2:1958880-1958899 MsG0280006439.01.T01:intron 25.0%
!! CAACATGAACAAATTTTGTA+GGG - Chr2:1959960-1959979 None:intergenic 25.0%
!! GGAATTTCATAGATTGATTT+AGG - Chr2:1960949-1960968 None:intergenic 25.0%
!! GTGAAAAGGTATATATATCT+AGG + Chr2:1959135-1959154 MsG0280006439.01.T01:intron 25.0%
!! TAATTATTTAAGATCACCTC+AGG - Chr2:1958619-1958638 None:intergenic 25.0%
!! TCAACATGAACAAATTTTGT+AGG - Chr2:1959961-1959980 None:intergenic 25.0%
!!! AATGTTTTGGAAAATTTCTC+AGG + Chr2:1961423-1961442 MsG0280006439.01.T01:intron 25.0%
!!! ATTTTTGTCGATTTAAGACT+TGG - Chr2:1959765-1959784 None:intergenic 25.0%
!!! TTTTTGTCGATTTAAGACTT+GGG - Chr2:1959764-1959783 None:intergenic 25.0%
AAATGTCGAAATTGCTAAGT+CGG + Chr2:1959781-1959800 MsG0280006439.01.T01:intron 30.0%
AACAATACAACAACGACAAA+AGG + Chr2:1960076-1960095 MsG0280006439.01.T01:intron 30.0%
AACAATTGTAACATCAAGCA+TGG + Chr2:1958861-1958880 MsG0280006439.01.T01:CDS 30.0%
AACATAGAATACATGACATG+TGG + Chr2:1960730-1960749 MsG0280006439.01.T01:CDS 30.0%
AATGTTATAGACCTTGGAAT+TGG + Chr2:1958817-1958836 MsG0280006439.01.T01:CDS 30.0%
ACACACATAATTACTATTCC+AGG + Chr2:1961610-1961629 MsG0280006439.01.T01:CDS 30.0%
ACAGAAAATTCTTATGAAGC+AGG + Chr2:1960639-1960658 MsG0280006439.01.T01:intron 30.0%
ACATAGAATACATGACATGT+GGG + Chr2:1960731-1960750 MsG0280006439.01.T01:CDS 30.0%
ACCAGACATTACTTACATTT+CGG - Chr2:1959207-1959226 None:intergenic 30.0%
AGAATGAAACTAAGAGGTTA+AGG - Chr2:1961051-1961070 None:intergenic 30.0%
ATAGACTCAAGTGTCATAAT+TGG + Chr2:1960667-1960686 MsG0280006439.01.T01:CDS 30.0%
ATGCTTGATGTTACAATTGT+TGG - Chr2:1958862-1958881 None:intergenic 30.0%
CACACATAATTACTATTCCA+GGG + Chr2:1961611-1961630 MsG0280006439.01.T01:CDS 30.0%
CATACCAATGCATAACTTTA+TGG + Chr2:1960359-1960378 MsG0280006439.01.T01:intron 30.0%
CATGCAAAAACATACTTCTT+TGG - Chr2:1960977-1960996 None:intergenic 30.0%
CTAGATCATTTATTAAGGGA+CGG + Chr2:1960545-1960564 MsG0280006439.01.T01:intron 30.0%
CTAGGTCATTTATTAAGAGA+CGG - Chr2:1960325-1960344 None:intergenic 30.0%
CTGACAAAATGAATGTTACA+AGG - Chr2:1959991-1960010 None:intergenic 30.0%
GAAAATTCACAAGTAAGTTG+AGG - Chr2:1959836-1959855 None:intergenic 30.0%
GACAAGTTATACAGGTTATT+AGG - Chr2:1961301-1961320 None:intergenic 30.0%
GCTATAAGCTATTATTGTCA+TGG + Chr2:1958740-1958759 MsG0280006439.01.T01:intron 30.0%
GTAAAAATGCACCATATGTT+TGG + Chr2:1961533-1961552 MsG0280006439.01.T01:CDS 30.0%
TGATGTTACAATTGTTGGTA+AGG - Chr2:1958857-1958876 None:intergenic 30.0%
TGTACAGATATCTTCAGATT+GGG - Chr2:1959498-1959517 None:intergenic 30.0%
TGTTAACCCTTCAGATTATT+CGG + Chr2:1959174-1959193 MsG0280006439.01.T01:intron 30.0%
TTTGCTTACAAGCATATTTG+TGG + Chr2:1960204-1960223 MsG0280006439.01.T01:intron 30.0%
! ATTGCTGCCAAATAATGTTT+TGG + Chr2:1961410-1961429 MsG0280006439.01.T01:intron 30.0%
! GATGTTACAATTGTTGGTAA+GGG - Chr2:1958856-1958875 None:intergenic 30.0%
! GTAATATTTTGCATTAGTCG+AGG - Chr2:1961359-1961378 None:intergenic 30.0%
!! ACAATATTATTGCTTGGTGT+CGG - Chr2:1960251-1960270 None:intergenic 30.0%
!! CAAAGAAGTATGTTTTTGCA+TGG + Chr2:1960975-1960994 MsG0280006439.01.T01:intron 30.0%
!! GGAATTTTGACAAGTTATAC+AGG - Chr2:1961309-1961328 None:intergenic 30.0%
AAAAACCTGAGTTCGATACT+TGG - Chr2:1959245-1959264 None:intergenic 35.0%
AAAACAATTGCAGCAACTTG+TGG + Chr2:1960852-1960871 MsG0280006439.01.T01:CDS 35.0%
AACATGGAGAAACTTCAACA+TGG + Chr2:1958583-1958602 MsG0280006439.01.T01:CDS 35.0%
ACATGGCTATTTATTTCCTG+AGG + Chr2:1958600-1958619 MsG0280006439.01.T01:CDS 35.0%
ACTAAGTCCTCTAAACTAAC+AGG + Chr2:1959638-1959657 MsG0280006439.01.T01:intron 35.0%
ACTCGAAATGAAGCACAAAT+AGG - Chr2:1961330-1961349 None:intergenic 35.0%
AGGTCTATAACATTTGCATG+TGG - Chr2:1958811-1958830 None:intergenic 35.0%
ATAAACTGTCAAACCAAGAG+AGG - Chr2:1961509-1961528 None:intergenic 35.0%
ATAATTACTATTCCAGGGAG+TGG + Chr2:1961616-1961635 MsG0280006439.01.T01:CDS 35.0%
ATCATTTATTAAGGGACGGA+GGG + Chr2:1960549-1960568 MsG0280006439.01.T01:intron 35.0%
ATGTGATATTTCTCGTCTTC+AGG + Chr2:1959363-1959382 MsG0280006439.01.T01:CDS 35.0%
CAAACTCCGAATAATCTGAA+GGG - Chr2:1959183-1959202 None:intergenic 35.0%
CAATCTGAAGATATCTGTAC+AGG + Chr2:1959497-1959516 MsG0280006439.01.T01:CDS 35.0%
CACTATTCTTTACAATGGCA+TGG + Chr2:1961022-1961041 MsG0280006439.01.T01:intron 35.0%
CATGCAAATGTTATAGACCT+TGG + Chr2:1958811-1958830 MsG0280006439.01.T01:CDS 35.0%
CTGTACAGATATCTTCAGAT+TGG - Chr2:1959499-1959518 None:intergenic 35.0%
CTTCAATGATAGAATCTCTC+TGG - Chr2:1961687-1961706 None:intergenic 35.0%
GAATATTGACAATACCGGAA+AGG - Chr2:1959529-1959548 None:intergenic 35.0%
GCAGAAGGTTATAAAGGATA+TGG + Chr2:1959101-1959120 MsG0280006439.01.T01:CDS 35.0%
GCATGCACTATTCTTTACAA+TGG + Chr2:1961017-1961036 MsG0280006439.01.T01:intron 35.0%
GGCAAGTATGTAGATAGAAT+TGG + Chr2:1960697-1960716 MsG0280006439.01.T01:CDS 35.0%
GGTTGAAATCATGCAAAACA+GGG - Chr2:1961180-1961199 None:intergenic 35.0%
GTACAAAGAAATCCAAGCAT+AGG - Chr2:1958976-1958995 None:intergenic 35.0%
GTCATTTATTAAGAGACGGA+GGG - Chr2:1960321-1960340 None:intergenic 35.0%
TATGACACTTGAGTCTATGT+AGG - Chr2:1960665-1960684 None:intergenic 35.0%
TATGCGTAATATGGTCAACT+AGG - Chr2:1960343-1960362 None:intergenic 35.0%
TCAACAGCAGAAGGTTATAA+AGG + Chr2:1959095-1959114 MsG0280006439.01.T01:CDS 35.0%
TTGTAGTATCACCAATTCCA+AGG - Chr2:1958831-1958850 None:intergenic 35.0%
! AAGATGTGTTTTCCTATGCT+TGG + Chr2:1958961-1958980 MsG0280006439.01.T01:intron 35.0%
! ATCTGAAACAAAAACCTCTG+AGG - Chr2:1959335-1959354 None:intergenic 35.0%
! ATGCATTGGTATGCGTAATA+TGG - Chr2:1960352-1960371 None:intergenic 35.0%
! ATTGTTTTCTTGTTGCTGCA+TGG - Chr2:1960840-1960859 None:intergenic 35.0%
! GGTTTTTCTGAACAACTTGT+AGG + Chr2:1959258-1959277 MsG0280006439.01.T01:intron 35.0%
! GTGGATTTTGCCAAAGTTAA+TGG + Chr2:1960871-1960890 MsG0280006439.01.T01:CDS 35.0%
! TCAATTGCCTGTTAGTTTAG+AGG - Chr2:1959648-1959667 None:intergenic 35.0%
! TCACAAGTAAGTTGAGGAAT+CGG - Chr2:1959830-1959849 None:intergenic 35.0%
! TCTCTTGGTTTGACAGTTTA+TGG + Chr2:1961508-1961527 MsG0280006439.01.T01:CDS 35.0%
! TCTGAAACAAAAACCTCTGA+GGG - Chr2:1959334-1959353 None:intergenic 35.0%
!! TCAGAGGTTTTTGTTTCAGA+TGG + Chr2:1959334-1959353 MsG0280006439.01.T01:CDS 35.0%
AAACACATGCAGCTGCTAAT+GGG + Chr2:1959471-1959490 MsG0280006439.01.T01:intron 40.0%
AAATACCTTCTCTACCAGGA+GGG + Chr2:1961721-1961740 MsG0280006439.01.T01:CDS 40.0%
AACCTTACATTAAGACCCTC+TGG - Chr2:1961271-1961290 None:intergenic 40.0%
AATAAGTTGTGCCCTTGAAG+TGG - Chr2:1960788-1960807 None:intergenic 40.0%
ACCTTACATTAAGACCCTCT+GGG - Chr2:1961270-1961289 None:intergenic 40.0%
ACTCAAATACCTTCTCTACC+AGG + Chr2:1961717-1961736 MsG0280006439.01.T01:CDS 40.0%
AGTGTCATAATTGGTCATGC+TGG + Chr2:1960676-1960695 MsG0280006439.01.T01:CDS 40.0%
ATAGTGTGTACCTGCTTCAA+TGG + Chr2:1961202-1961221 MsG0280006439.01.T01:CDS 40.0%
ATGACACTTCAATTGCAACC+TGG - Chr2:1961075-1961094 None:intergenic 40.0%
ATTGACAATACCGGAAAGGA+TGG - Chr2:1959525-1959544 None:intergenic 40.0%
CATGGAGAATGCAAGAATAC+TGG + Chr2:1961471-1961490 MsG0280006439.01.T01:CDS 40.0%
CCAAACTCCGAATAATCTGA+AGG - Chr2:1959184-1959203 None:intergenic 40.0%
CCTCTTAGTTTCATTCTTCC+AGG + Chr2:1961054-1961073 MsG0280006439.01.T01:intron 40.0%
CCTGGAAGAATGAAACTAAG+AGG - Chr2:1961057-1961076 None:intergenic 40.0%
CCTTCAGATTATTCGGAGTT+TGG + Chr2:1959181-1959200 MsG0280006439.01.T01:intron 40.0%
CGGTTGAAATCATGCAAAAC+AGG - Chr2:1961181-1961200 None:intergenic 40.0%
CTCCATGTAGTAGTCAACAA+GGG - Chr2:1961458-1961477 None:intergenic 40.0%
CTTTCCTGGCTCAAAATCTT+GGG + Chr2:1961561-1961580 MsG0280006439.01.T01:CDS 40.0%
GATCATTTATTAAGGGACGG+AGG + Chr2:1960548-1960567 MsG0280006439.01.T01:intron 40.0%
GCCGAAATGTAAGTAATGTC+TGG + Chr2:1959203-1959222 MsG0280006439.01.T01:intron 40.0%
GGTCATTTATTAAGAGACGG+AGG - Chr2:1960322-1960341 None:intergenic 40.0%
GTATTCTCAGACTGAATGCA+TGG + Chr2:1960596-1960615 MsG0280006439.01.T01:intron 40.0%
TCATCCTTCTCGAAATTTGC+CGG + Chr2:1961091-1961110 MsG0280006439.01.T01:CDS 40.0%
TCTCCATGTAGTAGTCAACA+AGG - Chr2:1961459-1961478 None:intergenic 40.0%
TGTTCACCAAACAATCCTAC+TGG + Chr2:1960814-1960833 MsG0280006439.01.T01:CDS 40.0%
TTCTCCATGTTTACATTCCG+AGG - Chr2:1958574-1958593 None:intergenic 40.0%
! AAACATACTTCTTTGGCTCC+AGG - Chr2:1960970-1960989 None:intergenic 40.0%
! AAGATTTTGAGCCAGGAAAG+CGG - Chr2:1961561-1961580 None:intergenic 40.0%
! AAGGTATTTGAGTCTCTCTG+AGG - Chr2:1961710-1961729 None:intergenic 40.0%
! ACAAGTAAGTTGAGGAATCG+GGG - Chr2:1959828-1959847 None:intergenic 40.0%
! ATGTTTAGGACACAGCACAA+AGG + Chr2:1958546-1958565 MsG0280006439.01.T01:CDS 40.0%
! CACAAGTAAGTTGAGGAATC+GGG - Chr2:1959829-1959848 None:intergenic 40.0%
! CTCATTTTGCAGTTTGTGCA+TGG + Chr2:1959068-1959087 MsG0280006439.01.T01:intron 40.0%
! CTTGGTTTGACAGTTTATGG+TGG + Chr2:1961511-1961530 MsG0280006439.01.T01:CDS 40.0%
! TATACCTTTTCACCTTGCTC+AGG - Chr2:1959128-1959147 None:intergenic 40.0%
!! TTCAACCAAGTATCGAACTC+AGG + Chr2:1959237-1959256 MsG0280006439.01.T01:intron 40.0%
AAAGGATATGGACCTGAGCA+AGG + Chr2:1959113-1959132 MsG0280006439.01.T01:CDS 45.0%
ATCCAACATATCTCAGGCTG+GGG + Chr2:1961228-1961247 MsG0280006439.01.T01:CDS 45.0%
ATCCGGGAAGAAGTCAGTTT+GGG - Chr2:1960758-1960777 None:intergenic 45.0%
CAAACACATGCAGCTGCTAA+TGG + Chr2:1959470-1959489 MsG0280006439.01.T01:intron 45.0%
CAAATACCTTCTCTACCAGG+AGG + Chr2:1961720-1961739 MsG0280006439.01.T01:CDS 45.0%
CAGCACTCTTGTTCTACAAG+AGG + Chr2:1959013-1959032 MsG0280006439.01.T01:intron 45.0%
CATCCAACATATCTCAGGCT+GGG + Chr2:1961227-1961246 MsG0280006439.01.T01:CDS 45.0%
CATGGTCTTTCAACAGCAGA+AGG + Chr2:1959086-1959105 MsG0280006439.01.T01:CDS 45.0%
CCTTACATTAAGACCCTCTG+GGG - Chr2:1961269-1961288 None:intergenic 45.0%
CTTATATACCCTTCACCACC+TGG - Chr2:1961649-1961668 None:intergenic 45.0%
CTTGAAGTGGTATGCAGATC+CGG - Chr2:1960775-1960794 None:intergenic 45.0%
GCTTTCCTGGCTCAAAATCT+TGG + Chr2:1961560-1961579 MsG0280006439.01.T01:CDS 45.0%
GGATCTGCATACCACTTCAA+GGG + Chr2:1960774-1960793 MsG0280006439.01.T01:CDS 45.0%
GGTACCTCGGAATGTAAACA+TGG + Chr2:1958567-1958586 MsG0280006439.01.T01:CDS 45.0%
TCTAGGGATAAAACCCTCAG+AGG + Chr2:1959318-1959337 MsG0280006439.01.T01:CDS 45.0%
TCTCAGACTGAATGCATGGT+TGG + Chr2:1960600-1960619 MsG0280006439.01.T01:intron 45.0%
TGAACAACTTGTAGGCTCTG+AGG + Chr2:1959266-1959285 MsG0280006439.01.T01:intron 45.0%
TGAAGCAGGTACACACTATG+CGG - Chr2:1961201-1961220 None:intergenic 45.0%
TGTACAGGATCCATCCTTTC+CGG + Chr2:1959512-1959531 MsG0280006439.01.T01:CDS 45.0%
TTGAAGTGGTATGCAGATCC+GGG - Chr2:1960774-1960793 None:intergenic 45.0%
! ACATCCCAAGATTTTGAGCC+AGG - Chr2:1961568-1961587 None:intergenic 45.0%
! ACTATTCCAGGGAGTGGTTT+TGG + Chr2:1961622-1961641 MsG0280006439.01.T01:CDS 45.0%
! CTCTTGTAGAACAAGAGTGC+TGG - Chr2:1959015-1959034 None:intergenic 45.0%
! GTCCCTTGTTGACTACTACA+TGG + Chr2:1961453-1961472 MsG0280006439.01.T01:CDS 45.0%
! TGGAGCATCCAACATATCTC+AGG + Chr2:1961222-1961241 MsG0280006439.01.T01:CDS 45.0%
ACTAGCATGTCTTTCCCCAG+AGG + Chr2:1961252-1961271 MsG0280006439.01.T01:CDS 50.0%
AGAATCCCTCCTGGTAGAGA+AGG - Chr2:1961729-1961748 None:intergenic 50.0%
CCCCAGAGGGTCTTAATGTA+AGG + Chr2:1961266-1961285 MsG0280006439.01.T01:intron 50.0%
CGGATCTGCATACCACTTCA+AGG + Chr2:1960773-1960792 MsG0280006439.01.T01:CDS 50.0%
CTAGCATGTCTTTCCCCAGA+GGG + Chr2:1961253-1961272 MsG0280006439.01.T01:CDS 50.0%
GAAGCCGGCAAATTTCGAGA+AGG - Chr2:1961098-1961117 None:intergenic 50.0%
GATCCGGGAAGAAGTCAGTT+TGG - Chr2:1960759-1960778 None:intergenic 50.0%
GCATCCAACATATCTCAGGC+TGG + Chr2:1961226-1961245 MsG0280006439.01.T01:CDS 50.0%
GCATGGCCAGTAGGATTGTT+TGG - Chr2:1960823-1960842 None:intergenic 50.0%
GGAAAGCGGACCCAAACATA+TGG - Chr2:1961547-1961566 None:intergenic 50.0%
GTCACTGCTTTGACCTCTCT+TGG + Chr2:1961493-1961512 MsG0280006439.01.T01:CDS 50.0%
GTTGGATGCTCCATTGAAGC+AGG - Chr2:1961215-1961234 None:intergenic 50.0%
TATGTTTGGGTCCGCTTTCC+TGG + Chr2:1961547-1961566 MsG0280006439.01.T01:CDS 50.0%
TCCAACATATCTCAGGCTGG+GGG + Chr2:1961229-1961248 MsG0280006439.01.T01:CDS 50.0%
TCCGGGAAGAAGTCAGTTTG+GGG - Chr2:1960757-1960776 None:intergenic 50.0%
TGGACCTGAGCAAGGTGAAA+AGG + Chr2:1959121-1959140 MsG0280006439.01.T01:CDS 50.0%
!! CAACTTGTAGGCTCTGAGGA+AGG + Chr2:1959270-1959289 MsG0280006439.01.T01:intron 50.0%
!! GACACAGCACAAAGGTACCT+CGG + Chr2:1958554-1958573 MsG0280006439.01.T01:CDS 50.0%
!! GTTTTGGTCCAGGTGGTGAA+GGG + Chr2:1961638-1961657 MsG0280006439.01.T01:CDS 50.0%
!! TCGAAATTTGCCGGCTTCAC+AGG + Chr2:1961100-1961119 MsG0280006439.01.T01:CDS 50.0%
AAGGCGAACACCTGTGAAGC+CGG - Chr2:1961113-1961132 None:intergenic 55.0%
CTTGTTGCTGCATGGCCAGT+AGG - Chr2:1960832-1960851 None:intergenic 55.0%
TCACAGGTGTTCGCCTTGGT+TGG + Chr2:1961116-1961135 MsG0280006439.01.T01:CDS 55.0%
TCCCCCAGCCTGAGATATGT+TGG - Chr2:1961233-1961252 None:intergenic 55.0%
TGTTCGCCTTGGTTGGACAG+TGG + Chr2:1961123-1961142 MsG0280006439.01.T01:CDS 55.0%
! GCCCCAAACTGACTTCTTCC+CGG + Chr2:1960753-1960772 MsG0280006439.01.T01:CDS 55.0%
!! GGTTTTGGTCCAGGTGGTGA+AGG + Chr2:1961637-1961656 MsG0280006439.01.T01:CDS 55.0%
CAGGGACCACTGTCCAACCA+AGG - Chr2:1961132-1961151 None:intergenic 60.0%
CCTGGACCAAAACCACTCCC+TGG - Chr2:1961631-1961650 None:intergenic 60.0%
GGCTTCACAGGTGTTCGCCT+TGG + Chr2:1961112-1961131 MsG0280006439.01.T01:CDS 60.0%
!!! CCAGGGAGTGGTTTTGGTCC+AGG + Chr2:1961628-1961647 MsG0280006439.01.T01:CDS 60.0%
!!! GGGAGTGGTTTTGGTCCAGG+TGG + Chr2:1961631-1961650 MsG0280006439.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 1958535 1961750 1958535 ID=MsG0280006439.01;Name=MsG0280006439.01
Chr2 mRNA 1958535 1961750 1958535 ID=MsG0280006439.01.T01;Parent=MsG0280006439.01;Name=MsG0280006439.01.T01;_AED=0.41;_eAED=0.41;_QI=0|0|0|1|1|1|8|0|418
Chr2 exon 1958535 1958621 1958535 ID=MsG0280006439.01.T01:exon:1281;Parent=MsG0280006439.01.T01
Chr2 exon 1958772 1958888 1958772 ID=MsG0280006439.01.T01:exon:1282;Parent=MsG0280006439.01.T01
Chr2 exon 1959080 1959142 1959080 ID=MsG0280006439.01.T01:exon:1283;Parent=MsG0280006439.01.T01
Chr2 exon 1959280 1959384 1959280 ID=MsG0280006439.01.T01:exon:1284;Parent=MsG0280006439.01.T01
Chr2 exon 1959483 1959533 1959483 ID=MsG0280006439.01.T01:exon:1285;Parent=MsG0280006439.01.T01
Chr2 exon 1960661 1960981 1960661 ID=MsG0280006439.01.T01:exon:1286;Parent=MsG0280006439.01.T01
Chr2 exon 1961076 1961282 1961076 ID=MsG0280006439.01.T01:exon:1287;Parent=MsG0280006439.01.T01
Chr2 exon 1961445 1961750 1961445 ID=MsG0280006439.01.T01:exon:1288;Parent=MsG0280006439.01.T01
Chr2 CDS 1958535 1958621 1958535 ID=MsG0280006439.01.T01:cds;Parent=MsG0280006439.01.T01
Chr2 CDS 1958772 1958888 1958772 ID=MsG0280006439.01.T01:cds;Parent=MsG0280006439.01.T01
Chr2 CDS 1959080 1959142 1959080 ID=MsG0280006439.01.T01:cds;Parent=MsG0280006439.01.T01
Chr2 CDS 1959280 1959384 1959280 ID=MsG0280006439.01.T01:cds;Parent=MsG0280006439.01.T01
Chr2 CDS 1959483 1959533 1959483 ID=MsG0280006439.01.T01:cds;Parent=MsG0280006439.01.T01
Chr2 CDS 1960661 1960981 1960661 ID=MsG0280006439.01.T01:cds;Parent=MsG0280006439.01.T01
Chr2 CDS 1961076 1961282 1961076 ID=MsG0280006439.01.T01:cds;Parent=MsG0280006439.01.T01
Chr2 CDS 1961445 1961750 1961445 ID=MsG0280006439.01.T01:cds;Parent=MsG0280006439.01.T01
Gene Sequence

>MsG0280006439.01.T01

ATGACTGTTAAATGTTTAGGACACAGCACAAAGGTACCTCGGAATGTAAACATGGAGAAACTTCAACATGGCTATTTATTTCCTGAGATTGAAAGACATGAGCTTCTTCACTTAAAGAAGTATCCACATGCAAATGTTATAGACCTTGGAATTGGTGATACTACAAAACCCTTACCAACAATTGTAACATCAAGCATGGTTGATTTTGTGCATGGTCTTTCAACAGCAGAAGGTTATAAAGGATATGGACCTGAGCAAGGTGAAAAGGCTCTGAGGAAGGCAATTGCTCATAAAGTTTACAAAGATCTAGGGATAAAACCCTCAGAGGTTTTTGTTTCAGATGGTGCACAATGTGATATTTCTCGTCTTCAGCTGCTAATGGGTCCCAATCTGAAGATATCTGTACAGGATCCATCCTTTCCGGCCTACATAGACTCAAGTGTCATAATTGGTCATGCTGGCAAGTATGTAGATAGAATTGGCAAATATGAAAACATAGAATACATGACATGTGGGCCCCAAACTGACTTCTTCCCGGATCTGCATACCACTTCAAGGGCACAACTTATTTTCTTCTGTTCACCAAACAATCCTACTGGCCATGCAGCAACAAGAAAACAATTGCAGCAACTTGTGGATTTTGCCAAAGTTAATGGATCAATCATTATCTATGACTCTGCATATTCTGCTTATATAACTGATGGTAGTCCTAAATCAATCTATGAAATTCCTGGAGCCAAAGAAGTTGCAATTGAAGTGTCATCCTTCTCGAAATTTGCCGGCTTCACAGGTGTTCGCCTTGGTTGGACAGTGGTCCCTGAAGAACTGTTATATTCAAACGGCTTCCCTGTTTTGCATGATTTCAACCGCATAGTGTGTACCTGCTTCAATGGAGCATCCAACATATCTCAGGCTGGGGGACTAGCATGTCTTTCCCCAGAGGGTCTTAATGCTGTGCAGTCCCTTGTTGACTACTACATGGAGAATGCAAGAATACTGGTCACTGCTTTGACCTCTCTTGGTTTGACAGTTTATGGTGGTAAAAATGCACCATATGTTTGGGTCCGCTTTCCTGGCTCAAAATCTTGGGATGTCTTTGCTGAAATTCTTGAGAATACACACATAATTACTATTCCAGGGAGTGGTTTTGGTCCAGGTGGTGAAGGGTATATAAGAATTAGTGCTTTTGGCCAGAGAGATTCTATCATTGAAGCCTCAGAGAGACTCAAATACCTTCTCTACCAGGAGGGATTCTAA
Protein sequence

>MsG0280006439.01.T01

MTVKCLGHSTKVPRNVNMEKLQHGYLFPEIERHELLHLKKYPHANVIDLGIGDTTKPLPTIVTSSMVDFVHGLSTAEGYKGYGPEQGEKALRKAIAHKVYKDLGIKPSEVFVSDGAQCDISRLQLLMGPNLKISVQDPSFPAYIDSSVIIGHAGKYVDRIGKYENIEYMTCGPQTDFFPDLHTTSRAQLIFFCSPNNPTGHAATRKQLQQLVDFAKVNGSIIIYDSAYSAYITDGSPKSIYEIPGAKEVAIEVSSFSKFAGFTGVRLGWTVVPEELLYSNGFPVLHDFNRIVCTCFNGASNISQAGGLACLSPEGLNAVQSLVDYYMENARILVTALTSLGLTVYGGKNAPYVWVRFPGSKSWDVFAEILENTHIITIPGSGFGPGGEGYIRISAFGQRDSIIEASERLKYLLYQEGF*