AlfalfaGEDB | MsG0280011022.01

M. sativa cultivar ZhongmuNo.1 / MsG0280011022.01

KEGG: -
GO: GO:0005515
PFAM: PF12796 PF13857

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011022.01.T01	XP_013465385.1	91.387	476	8	1	80	555	1	443	0	890

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011022.01.T01	Q94B55	63.071	482	132	5	80	550	1	447	0	611

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011022.01.T01	A0A072VMS8	91.387	476	8	1	80	555	1	443	0.0	890

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0080048156.01	MsG0280011022.01	PPI
MsG0080049065.01	MsG0280011022.01	PPI
MsG0180005811.01	MsG0280011022.01	PPI
MsG0180006146.01	MsG0280011022.01	PPI
MsG0280006326.01	MsG0280011022.01	PPI
MsG0280006373.01	MsG0280011022.01	PPI
MsG0280006398.01	MsG0280011022.01	PPI
MsG0280006439.01	MsG0280011022.01	PPI
MsG0280007849.01	MsG0280011022.01	PPI
MsG0280010541.01	MsG0280011022.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011022.01.T01	MTR_2g096760	91.387	476	8	1	80	555	1	443	0.0	890
MsG0280011022.01.T01	MTR_4g063830	69.167	480	109	6	80	555	1	445	0.0	669
MsG0280011022.01.T01	MTR_4g021880	54.672	503	172	11	64	550	59	521	7.37e-178	512
MsG0280011022.01.T01	MTR_4g021880	59.375	352	125	6	64	399	59	408	2.25e-139	410
MsG0280011022.01.T01	MTR_0020s0130	28.660	485	233	12	80	515	1	421	2.65e-39	150
MsG0280011022.01.T01	MTR_1g100797	28.636	440	219	12	80	488	1	376	2.55e-37	145
MsG0280011022.01.T01	MTR_4g129470	33.803	284	162	5	80	339	7	288	5.17e-31	127
MsG0280011022.01.T01	MTR_1g100797	27.410	332	147	11	188	488	4	272	1.03e-21	98.2
MsG0280011022.01.T01	MTR_1g115575	31.220	205	122	4	93	294	134	322	3.32e-18	88.6
MsG0280011022.01.T01	MTR_7g100390	30.859	256	137	10	76	298	30	278	2.46e-17	85.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011022.01.T01	AT2G28840	63.071	482	132	5	80	550	1	447	0.0	611
MsG0280011022.01.T01	AT2G28840	65.375	413	97	5	149	550	56	433	0.0	544
MsG0280011022.01.T01	AT5G07270	28.341	434	215	12	80	485	1	366	1.96e-36	142
MsG0280011022.01.T01	AT5G57740	28.111	434	220	9	80	485	7	376	4.70e-31	127
MsG0280011022.01.T01	AT5G02620	28.819	288	166	13	89	356	55	323	7.73e-19	90.1
MsG0280011022.01.T01	AT5G02620	28.819	288	166	13	89	356	55	323	7.73e-19	90.1
MsG0280011022.01.T01	AT5G02620	28.819	288	166	13	89	356	55	323	7.73e-19	90.1
MsG0280011022.01.T01	AT5G02620	29.808	208	121	8	89	290	55	243	3.58e-17	85.1
MsG0280011022.01.T01	AT5G02620	29.808	208	121	8	89	290	55	243	3.58e-17	85.1
MsG0280011022.01.T01	AT3G04710	31.154	260	146	8	101	333	25	278	3.86e-17	84.7

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Find 119 sgRNAs with CRISPR-Local

Find 416 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTTCACCTCTGGTTTGAA+TGG	0.356592	2:-78518597	None:intergenic
AAGAAATAGCATTCTCATTG+TGG	0.517089	2:+78520677	MsG0280011022.01.T01:CDS
AAGACCTTGACCCATGTTGT+CGG	0.549293	2:-78518643	None:intergenic
AAGCAACATAGTTAGACTTA+TGG	0.364923	2:+78520884	MsG0280011022.01.T01:CDS
AAGGGAGAAAGATATATTGA+AGG	0.462497	2:+78520358	MsG0280011022.01.T01:CDS
AAGTGGTTGAACTTCACCTC+TGG	0.492479	2:-78518606	None:intergenic
AAGTTGGGCGTCCGAAGTTC+AGG	0.350071	2:+78521053	MsG0280011022.01.T01:CDS
AATAGAATATGTACACATTC+AGG	0.364281	2:-78519565	None:intergenic
ACAAGAGGCTCAGCAGATGT+AGG	0.576412	2:-78520248	None:intergenic
ACAATTGAAGTTCAGAATTG+TGG	0.536648	2:+78520762	MsG0280011022.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACGTGTGCCTAAATAATT+TGG	-	Chr2:78515213-78515232	None:intergenic	30.0%
AACGTGTGCCTAAATAATTT+GGG	-	Chr2:78515212-78515231	None:intergenic	30.0%
AAGAAATAGCATTCTCATTG+TGG	+	Chr2:78520677-78520696	MsG0280011022.01.T01:CDS	30.0%
AAGCAACATAGTTAGACTTA+TGG	+	Chr2:78520884-78520903	MsG0280011022.01.T01:CDS	30.0%
AAGGGAGAAAGATATATTGA+AGG	+	Chr2:78520358-78520377	MsG0280011022.01.T01:CDS	30.0%
AATGTCGTTATATTTGATGG+AGG	-	Chr2:78518054-78518073	None:intergenic	30.0%
ACAAAGATCCTAACACTTAA+TGG	-	Chr2:78517833-78517852	None:intergenic	30.0%
ACAATTGAAGTTCAGAATTG+TGG	+	Chr2:78520762-78520781	MsG0280011022.01.T01:CDS	30.0%
ACAGAGTTGTTACAATTTGA+AGG	-	Chr2:78518722-78518741	None:intergenic	30.0%
ACATAATAGTCTCACACTTA+AGG	-	Chr2:78517076-78517095	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	78513951	78521109	78513951	ID=MsG0280011022.01;Name=MsG0280011022.01
Chr2	mRNA	78513951	78521109	78513951	ID=MsG0280011022.01.T01;Parent=MsG0280011022.01;Name=MsG0280011022.01.T01;_AED=0.49;_eAED=0.57;_QI=0\|0.75\|0.66\|1\|1\|1\|9\|0\|555
Chr2	exon	78513951	78514124	78513951	ID=MsG0280011022.01.T01:exon:21268;Parent=MsG0280011022.01.T01
Chr2	exon	78518588	78518830	78518588	ID=MsG0280011022.01.T01:exon:21269;Parent=MsG0280011022.01.T01
Chr2	exon	78518940	78518999	78518940	ID=MsG0280011022.01.T01:exon:21270;Parent=MsG0280011022.01.T01
Chr2	exon	78519103	78519174	78519103	ID=MsG0280011022.01.T01:exon:21271;Parent=MsG0280011022.01.T01
Chr2	exon	78519264	78519385	78519264	ID=MsG0280011022.01.T01:exon:21272;Parent=MsG0280011022.01.T01
Chr2	exon	78519489	78519629	78519489	ID=MsG0280011022.01.T01:exon:21273;Parent=MsG0280011022.01.T01
Chr2	exon	78519729	78519836	78519729	ID=MsG0280011022.01.T01:exon:21274;Parent=MsG0280011022.01.T01
Chr2	exon	78520187	78520442	78520187	ID=MsG0280011022.01.T01:exon:21275;Parent=MsG0280011022.01.T01
Chr2	exon	78520618	78521109	78520618	ID=MsG0280011022.01.T01:exon:21276;Parent=MsG0280011022.01.T01
Chr2	CDS	78513951	78514124	78513951	ID=MsG0280011022.01.T01:cds;Parent=MsG0280011022.01.T01
Chr2	CDS	78518588	78518830	78518588	ID=MsG0280011022.01.T01:cds;Parent=MsG0280011022.01.T01
Chr2	CDS	78518940	78518999	78518940	ID=MsG0280011022.01.T01:cds;Parent=MsG0280011022.01.T01
Chr2	CDS	78519103	78519174	78519103	ID=MsG0280011022.01.T01:cds;Parent=MsG0280011022.01.T01
Chr2	CDS	78519264	78519385	78519264	ID=MsG0280011022.01.T01:cds;Parent=MsG0280011022.01.T01
Chr2	CDS	78519489	78519629	78519489	ID=MsG0280011022.01.T01:cds;Parent=MsG0280011022.01.T01
Chr2	CDS	78519729	78519836	78519729	ID=MsG0280011022.01.T01:cds;Parent=MsG0280011022.01.T01
Chr2	CDS	78520187	78520442	78520187	ID=MsG0280011022.01.T01:cds;Parent=MsG0280011022.01.T01
Chr2	CDS	78520618	78521109	78520618	ID=MsG0280011022.01.T01:cds;Parent=MsG0280011022.01.T01

Gene Sequence

>MsG0280011022.01.T01

ATGTTAATTCCAGGTGTTCGTACAACCGGTTCATCATTCAATATTCCATTGAAAATCAATGAAGTATTGATACATGGATTTGAAAAGAAAATACGAGACCTTAAAATTGATATGAAGGCAGCAGAGAGAATCAAACACGAGACCTTAAAAGTAGTCCCAGGCCAAAACAAGTGGGTGGCTCATCCATTCAAACCAGAGGTGAAGTTCAACCACTTAACTTCCCCTCTCCCCGACAACATGGGTCAAGGTCTTAGTTGCAGAGGTAACAATGATCATGGTCTCTTCATTGCTGTCCAACAAGGTAACCTTCAAATTGTAACAACTCTGTTACAACAAGACCCTTCTCTTTTCCACCAAAAAACTCTCTTTGATCGTTTTTCACCTCTTCATATTGCTGCTGCTAATGGCCAGATCGAGATTCTGTCAAGGCTTTTACATGGATCTGTTAATCCAGATGTTTTAAATCGAAAAAAACAGACTCCTCTTATGTTGGCTGCAATGCATGGAAGGATTGCATGTGTGGAGAAGCTACTTGAAGCTGGTGCTAATGTTTTGATGTTTGATACGGTTAATGGAAGAACTTGTTTACACTATGCTGCTTATTATGGCCATTTTTCTTGCCTTAAAGCTATTCTTTCTTCTGCTCAATCTAGTCATGTGGCTGCTTCTTGGGGATTTGTTCGGTTTGTGAATGTTAGAGATGGAAAGGGTGCGACGCCATTGCATTTGGCATCTCGACAAAGACGGCCTGAATGTGTACATATTCTATTAGACAGTGGAGCTCTTGTTTGTGCTTCGACCGGTAGATATGGCTATCCTGGAAGCACTCCCCTTCATCTTGCTGCTAGAGGGGGGTCTCTTGATTGCATCCGTGAATTGTTGGCATGGGGCGCAGATCGTATTCAGCGTGATTCATCTGGGCGTATACCATACATGGTTGCTATGAAACACAGACACGGATCATGCACGTCCTTGCTAAATCCTACATCTGCTGAGCCTCTTGTTTGGCCATCTCCACTGAAATTCATCAGTGAACTTAATCCGGAAGCCAAAGCCTTGTTAGAACAAGCTTTGATGGATGCAAACAGAGAAAGGGAGAAAGATATATTGAAGGGGAAATTTTACTCTCTTCCGTCTCCATCACAATCAGATTGTGTAGATGACAATATCTCCGAGGCTGAGAGATTTGATTATTTGGCCAAGTTTAAAATTTGCATTATAAATGTGATCCAAAAAAGAAATAGCATTCTCATTGTGGAATATGATATTATGCAGGTCAGTGAGTCAGAATTATGCTGCATCTGCTTTGAGCAGGTATGCACAATTGAAGTTCAGAATTGTGGTCACCAAATGTGTGCACAATGCACATTATCCCTTTGTTGCTACAACAAACCGAACCCGACAACTACTTCTATAACTCCACCAGTTTGTCCATTTTGTCGAAGCAACATAGTTAGACTTATGGTTGTCATGAAAATTGAAAATCACGACGAAACTGATCAAGACAACGTTGATATCAATTGTTCAAAGATAAACAAGTTGAGGAAACTCCGAAATTTGAACGACAGCGGTAGTAGCAGCTTCAAAGGATTATCTAGTGTGTCGTTCGGAAAGTTGGGCGTCCGAAGTTCAGGAAGGGTTGCTGCTGAATGGATTGATAAAGAATGA

Protein sequence

>MsG0280011022.01.T01

MLIPGVRTTGSSFNIPLKINEVLIHGFEKKIRDLKIDMKAAERIKHETLKVVPGQNKWVAHPFKPEVKFNHLTSPLPDNMGQGLSCRGNNDHGLFIAVQQGNLQIVTTLLQQDPSLFHQKTLFDRFSPLHIAAANGQIEILSRLLHGSVNPDVLNRKKQTPLMLAAMHGRIACVEKLLEAGANVLMFDTVNGRTCLHYAAYYGHFSCLKAILSSAQSSHVAASWGFVRFVNVRDGKGATPLHLASRQRRPECVHILLDSGALVCASTGRYGYPGSTPLHLAARGGSLDCIRELLAWGADRIQRDSSGRIPYMVAMKHRHGSCTSLLNPTSAEPLVWPSPLKFISELNPEAKALLEQALMDANREREKDILKGKFYSLPSPSQSDCVDDNISEAERFDYLAKFKICIINVIQKRNSILIVEYDIMQVSESELCCICFEQVCTIEVQNCGHQMCAQCTLSLCCYNKPNPTTTSITPPVCPFCRSNIVRLMVVMKIENHDETDQDNVDINCSKINKLRKLRNLNDSGSSSFKGLSSVSFGKLGVRSSGRVAAEWIDKE*