AlfalfaGEDB | MsG0280006472.01

M. sativa cultivar ZhongmuNo.1 / MsG0280006472.01

KEGG: K01626
GO: GO:0003824 GO:0003849 GO:0009073
PFAM: PF01474

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006472.01.T01	XP_003593221.1	97.456	511	12	1	1	511	1	510	0	1034
MsG0280006472.01.T02	XP_003593221.1	97.456	511	12	1	1	511	1	510	0	1034

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006472.01.T01	A0A067XGX8	84.778	473	72	0	33	505	40	512	0	856
MsG0280006472.01.T02	A0A067XGX8	84.778	473	72	0	33	505	40	512	0	856

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006472.01.T01	G7IPJ9	97.456	511	12	1	1	511	1	510	0.0	1034
MsG0280006472.01.T02	G7IPJ9	97.456	511	12	1	1	511	1	510	0.0	1034

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180001880.01	MsG0280006472.01	0.801306	9.584248e-49	4.561818e-46
MsG0280006472.01	MsG0580024944.01	0.808488	3.021559e-50	1.729937e-47
MsG0280006472.01	MsG0580028111.01	0.814398	1.572629e-51	1.054115e-48

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MsG0280006472.01.T01	MTR_2g009080	97.456	511	12	1	1	511	1	510	1034
MsG0280006472.01.T01	MTR_5g064500	83.547	468	77	0	40	507	62	529	842
MsG0280006472.01.T01	MTR_5g064500	82.687	387	67	0	36	422	58	444	690
MsG0280006472.01.T02	MTR_2g009080	97.456	511	12	1	1	511	1	510	1034
MsG0280006472.01.T02	MTR_5g064500	83.547	468	77	0	40	507	62	529	842
MsG0280006472.01.T02	MTR_5g064500	82.687	387	67	0	36	422	58	444	690

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MsG0280006472.01.T01	AT4G33510	83.264	484	77	2	20	501	17	498	848
MsG0280006472.01.T01	AT1G22410	83.407	452	75	0	55	506	75	526	821
MsG0280006472.01.T01	AT4G39980	75.460	489	114	2	19	501	33	521	802
MsG0280006472.01.T01	AT4G33510	79.755	326	62	2	20	343	17	340	549
MsG0280006472.01.T02	AT4G33510	83.264	484	77	2	20	501	17	498	848
MsG0280006472.01.T02	AT1G22410	83.407	452	75	0	55	506	75	526	821
MsG0280006472.01.T02	AT4G39980	75.460	489	114	2	19	501	33	521	802
MsG0280006472.01.T02	AT4G33510	79.755	326	62	2	20	343	17	340	549

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Find 26 sgRNAs with CRISPR-Local

Find 405 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACGTGACAGAATGTGTTGG+AGG	0.597335	2:-2455232	MsG0280006472.01.T01:CDS
AAGAAGGAAGCTACCCTGGA+GGG	0.566905	2:-2455279	MsG0280006472.01.T01:CDS
ACACTAACGTTTCTGACACA+TGG	0.539959	2:+2455021	None:intergenic
ACGTGACAGAATGTGTTGGA+GGG	0.645550	2:-2455231	MsG0280006472.01.T01:CDS
AGAAGGAAGCTACCCTGGAG+GGG	0.616659	2:-2455278	MsG0280006472.01.T01:CDS
ATGTATATTTAAGTATGCAA+TGG	0.599490	2:-2454995	MsG0280006472.01.T01:three_prime_UTR
ATTTCCAAATGAACCCCTCC+AGG	0.482948	2:+2455265	None:intergenic
CAAAACGTGACAGAATGTGT+TGG	0.647713	2:-2455235	MsG0280006472.01.T01:CDS
CAACTCTGTATGTGTGAGTT+TGG	0.362671	2:-2454925	MsG0280006472.01.T01:three_prime_UTR
CAAGAAGGAAGCTACCCTGG+AGG	0.704845	2:-2455280	MsG0280006472.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAGGCTGAAAACGTAAA+CGG	-	Chr2:2459760-2459779	MsG0280006472.01.T01:five_prime_UTR	30.0%
AAACAAAGACAGATGGAAAT+AGG	+	Chr2:2458595-2458614	None:intergenic	30.0%
AAAGAAGAAACAAAGACAGA+TGG	+	Chr2:2458602-2458621	None:intergenic	30.0%
AAGAATTAGCTGCTACTAAA+TGG	-	Chr2:2457227-2457246	MsG0280006472.01.T01:CDS	30.0%
ACAATTACTGTAATTCTTCC+AGG	+	Chr2:2457334-2457353	None:intergenic	30.0%
ACAGGGAAAAAAATTGGAAT+GGG	-	Chr2:2456400-2456419	MsG0280006472.01.T01:intron	30.0%
ACAGTTAGTTAATTAGTTGG+TGG	+	Chr2:2458096-2458115	None:intergenic	30.0%
AGTATGATTAAAGACGTTTC+AGG	-	Chr2:2459627-2459646	MsG0280006472.01.T01:CDS	30.0%
ATAGCAACTTAGACAAACAT+AGG	+	Chr2:2457007-2457026	None:intergenic	30.0%
ATTATCAGCTATGTAGAAGA+GGG	+	Chr2:2458336-2458355	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	2454131	2459883	2454131	ID=MsG0280006472.01;Name=MsG0280006472.01
Chr2	mRNA	2454131	2459883	2454131	ID=MsG0280006472.01.T01;Parent=MsG0280006472.01;Name=MsG0280006472.01.T01;_AED=0.37;_eAED=0.37;_QI=153\|1\|1\|1\|0.8\|0.66\|6\|304\|511
Chr2	exon	2454131	2454176	2454131	ID=MsG0280006472.01.T01:exon:403;Parent=MsG0280006472.01.T01
Chr2	exon	2454808	2455335	2454808	ID=MsG0280006472.01.T01:exon:402;Parent=MsG0280006472.01.T01
Chr2	five_prime_UTR	2459731	2459883	2459731	ID=MsG0280006472.01.T01:five_prime_utr;Parent=MsG0280006472.01.T01
Chr2	CDS	2459266	2459730	2459266	ID=MsG0280006472.01.T01:cds;Parent=MsG0280006472.01.T01
Chr2	CDS	2458180	2458457	2458180	ID=MsG0280006472.01.T01:cds;Parent=MsG0280006472.01.T01
Chr2	CDS	2457032	2457308	2457032	ID=MsG0280006472.01.T01:cds;Parent=MsG0280006472.01.T01
Chr2	CDS	2456504	2456749	2456504	ID=MsG0280006472.01.T01:cds;Parent=MsG0280006472.01.T01
Chr2	CDS	2455066	2455335	2455066	ID=MsG0280006472.01.T01:cds;Parent=MsG0280006472.01.T01
Chr2	three_prime_UTR	2454808	2455065	2454808	ID=MsG0280006472.01.T01:three_prime_utr;Parent=MsG0280006472.01.T01
Chr2	three_prime_UTR	2454131	2454176	2454131	ID=MsG0280006472.01.T01:three_prime_utr;Parent=MsG0280006472.01.T01
Chr2	mRNA	2454794	2459883	2454794	ID=MsG0280006472.01.T02;Parent=MsG0280006472.01;Name=MsG0280006472.01.T02;_AED=0.37;_eAED=0.37;_QI=153\|1\|1\|1\|1\|1\|5\|272\|511
Chr2	exon	2454794	2455335	2454794	ID=MsG0280006472.01.T02:exon:404;Parent=MsG0280006472.01.T02
Chr2	five_prime_UTR	2459731	2459883	2459731	ID=MsG0280006472.01.T02:five_prime_utr;Parent=MsG0280006472.01.T02
Chr2	CDS	2459266	2459730	2459266	ID=MsG0280006472.01.T02:cds;Parent=MsG0280006472.01.T02
Chr2	CDS	2458180	2458457	2458180	ID=MsG0280006472.01.T02:cds;Parent=MsG0280006472.01.T02
Chr2	CDS	2457032	2457308	2457032	ID=MsG0280006472.01.T02:cds;Parent=MsG0280006472.01.T02
Chr2	CDS	2456504	2456749	2456504	ID=MsG0280006472.01.T02:cds;Parent=MsG0280006472.01.T02
Chr2	CDS	2455066	2455335	2455066	ID=MsG0280006472.01.T02:cds;Parent=MsG0280006472.01.T02
Chr2	three_prime_UTR	2454794	2455065	2454794	ID=MsG0280006472.01.T02:three_prime_utr;Parent=MsG0280006472.01.T02

Gene Sequence

>MsG0280006472.01.T01

ATGTCTTTAACATCTGCATCCCTAATCTCTTTTACACCCTCCCTATCAATACCACACCCCCCTCGTCATCACCAACGCAGATCGATAATCGTCGCAAATTCCTCTTCTTCTTCCGACGCTAAAAATTCAATAGCAACAACTTCACGATTATCAACCACCCCATGGAACATAGATAGTTGGAGAACAAAGAAGGCACTGCAACTTCCAGAATATCCAGATCAGGGACAATTAGATGAAGTTCTTCAAACCCTCTCTTCTTTCCCACCAATTGTTTTTGCTGGTGAAGCTAGAAATCTTGAGGATAAACTCGCTCAAGCTTCCATGGGGAACGCTTTTCTTCTCATGGGTGGTGATTGTGCTGAGAGCTTTAAGGAGTTTAGTGCGAATAATATTCGTGATACTTTTCGTGTTATTCTTCAAATGGGTGTTGTTCTTATGTTTGGTGCTCAAATGCCTGTTATTAAGGTGGGTAGAATGGCGGGTCAGTTTGCAAAGCCAAGATCGGATTCATTTGAGGAGAAAAATGGAGTCAAGCTGCCAAGTTACAGGGGTGACAATGTGAATGGGGATGCATTTGATGCAGCATCTAGAATTCCAGATCCACAGAGAATGGTAAGAGCCTACTGCCAGTCTGTGGCTACTCTCAACCTTTTGCGGGCGTTTGCTACTGGAGGTTATGCTGCCATGCAAAGAGTCAACCAGTGGAATCTTGATTTCATGGAGCAGAGTGAACAGGGAGACAGGTACCGTGAATTGGCACATCGAGTGGATGAGGCTCTTGGCTTCATGAGTGTTGCTGGAATTACATCTGACCATCCAATCATGACTACAACAGACTTTTGGACCTCCCATGAGTGTTTGCTTCTCCCTTACGAACAAGCACTTACTAGGGAAGATTCTACTTCTGGGCTTAATTATGATTGCTCAGCTCACATGCTGTGGGTTGGGGAACGTACCCGCCAACTTGATGGTGCTCATGTTGAATTCTTGAGAGGAGTTGCTAATCCCCTTGGCATTAAGGTTAGTGATAAGATGGATCCAAATGAACTGGTTAAGCTGATAGATATTCTGAACCCCAATAACAAGCCTGGAAGAATTACAGTAATTGTAAGAATGGGAGCCGAGAATATGCGAGTGAAGCTTCCACATCTTATCAGGGCAGTTCGCAATGCTGGTCAAATTGTCGCTTGGGTTAGTGATCCCATGCATGGGAACACCATCAAAGCTCCATCGGGACTTAAAACCCGATCTTTTGATTCAATAAGGGCTGAACTGAGGGCATTCTTTGATGTCCACGAGCAAGAAGGAAGCTACCCTGGAGGGGTTCATTTGGAAATGACTGGTCAAAACGTGACAGAATGTGTTGGAGGGTCACGGACTATTACTTTTGATGACTTGAGTTCACGCTACCACACTCATTGTGATCCAAGACTCAATGCTTCTCAATCACTTGAGCTTGCGTTCGCTATCGCAGAAAGACTGCGCAAGAGAAGGCTAAACTCAACTCAATCGCTGTCTTCCGCAGTCAGAATTTAG

>MsG0280006472.01.T02

Protein sequence

>MsG0280006472.01.T01

MSLTSASLISFTPSLSIPHPPRHHQRRSIIVANSSSSSDAKNSIATTSRLSTTPWNIDSWRTKKALQLPEYPDQGQLDEVLQTLSSFPPIVFAGEARNLEDKLAQASMGNAFLLMGGDCAESFKEFSANNIRDTFRVILQMGVVLMFGAQMPVIKVGRMAGQFAKPRSDSFEEKNGVKLPSYRGDNVNGDAFDAASRIPDPQRMVRAYCQSVATLNLLRAFATGGYAAMQRVNQWNLDFMEQSEQGDRYRELAHRVDEALGFMSVAGITSDHPIMTTTDFWTSHECLLLPYEQALTREDSTSGLNYDCSAHMLWVGERTRQLDGAHVEFLRGVANPLGIKVSDKMDPNELVKLIDILNPNNKPGRITVIVRMGAENMRVKLPHLIRAVRNAGQIVAWVSDPMHGNTIKAPSGLKTRSFDSIRAELRAFFDVHEQEGSYPGGVHLEMTGQNVTECVGGSRTITFDDLSSRYHTHCDPRLNASQSLELAFAIAERLRKRRLNSTQSLSSAVRI*

>MsG0280006472.01.T02