Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007194.01.T01 | XP_013462733.1 | 100 | 58 | 0 | 0 | 7 | 64 | 256 | 313 | 2.07E-32 | 125 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007194.01.T01 | Q8LA32 | 60 | 60 | 23 | 1 | 9 | 68 | 229 | 287 | 1.81E-16 | 74.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007194.01.T01 | A0A072V5Z5 | 100.000 | 58 | 0 | 0 | 7 | 64 | 256 | 313 | 9.88e-33 | 125 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003942.01 | MsG0280007194.01 | 0.803159 | 3.981075e-49 | 1.986326e-46 |
| MsG0280007193.01 | MsG0280007194.01 | 0.815911 | 7.250928e-52 | 5.064137e-49 |
| MsG0280007194.01 | MsG0480021822.01 | 0.810716 | 1.003649e-50 | 6.095151e-48 |
| MsG0280007194.01 | MsG0580025561.01 | 0.825404 | 4.775165e-54 | 4.348265e-51 |
| MsG0280007194.01 | MsG0680032473.01 | 0.808602 | 2.857795e-50 | 1.640965e-47 |
| MsG0280007194.01 | MsG0880047422.01 | 0.813137 | 2.980529e-51 | 1.930410e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007194.01.T01 | MTR_2g022210 | 100.000 | 58 | 0 | 0 | 7 | 64 | 256 | 313 | 2.50e-36 | 125 |
| MsG0280007194.01.T01 | MTR_4g117250 | 78.846 | 52 | 11 | 0 | 13 | 64 | 234 | 285 | 4.12e-23 | 90.1 |
| MsG0280007194.01.T01 | MTR_1g064680 | 50.877 | 57 | 23 | 2 | 8 | 64 | 223 | 274 | 3.02e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007194.01.T01 | AT3G06140 | 60.000 | 60 | 23 | 1 | 9 | 68 | 229 | 287 | 1.84e-17 | 74.7 |
| MsG0280007194.01.T01 | AT5G19080 | 57.143 | 56 | 23 | 1 | 9 | 64 | 245 | 299 | 6.84e-14 | 64.7 |
Find 16 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAAATTACTCAAGCTGTCT+TGG | 0.394705 | 2:+11691656 | MsG0280007194.01.T01:CDS |
| TATGAGTTGAGAGAACTATA+TGG | 0.439020 | 2:+11691750 | MsG0280007194.01.T01:CDS |
| AATATGCGCTGAAACATGTT+TGG | 0.444189 | 2:+11691567 | None:intergenic |
| AGACAGATTCTGTGGATTGA+TGG | 0.470345 | 2:+11691720 | MsG0280007194.01.T01:CDS |
| GCATCTAGCACGGAATCATC+AGG | 0.477136 | 2:-11691621 | None:intergenic |
| CTTGAGTAATTTGCATGTGA+GGG | 0.532696 | 2:-11691647 | None:intergenic |
| GTTGAGAGAACTATATGGAA+AGG | 0.541467 | 2:+11691755 | MsG0280007194.01.T01:CDS |
| AAACATGTTTGGAAACTCCC+TGG | 0.545471 | 2:+11691578 | None:intergenic |
| AAAGGAAGCTCAGAAGCTGC+AGG | 0.551301 | 2:+11691773 | MsG0280007194.01.T01:CDS |
| GCTTGAGTAATTTGCATGTG+AGG | 0.568254 | 2:-11691648 | None:intergenic |
| AAGTAGTTAGACAGATTCTG+TGG | 0.607111 | 2:+11691712 | MsG0280007194.01.T01:CDS |
| GCTGTCTTGGAGAAAAGTAA+TGG | 0.618568 | 2:+11691669 | MsG0280007194.01.T01:CDS |
| GTGAGGGGATGCATCTAGCA+CGG | 0.624270 | 2:-11691631 | None:intergenic |
| TTGGAGAAAAGTAATGGTGG+TGG | 0.678741 | 2:+11691675 | MsG0280007194.01.T01:CDS |
| TTGAGTAATTTGCATGTGAG+GGG | 0.691901 | 2:-11691646 | None:intergenic |
| GTCTTGGAGAAAAGTAATGG+TGG | 0.719546 | 2:+11691672 | MsG0280007194.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TATGAGTTGAGAGAACTATA+TGG | + | Chr2:11691750-11691769 | MsG0280007194.01.T01:CDS | 30.0% |
| !! | TCTGTCTAACTACTTTTATC+TGG | - | Chr2:11691707-11691726 | None:intergenic | 30.0% |
| AAGTAGTTAGACAGATTCTG+TGG | + | Chr2:11691712-11691731 | MsG0280007194.01.T01:CDS | 35.0% | |
| GTTGAGAGAACTATATGGAA+AGG | + | Chr2:11691755-11691774 | MsG0280007194.01.T01:CDS | 35.0% | |
| ! | CTTGAGTAATTTGCATGTGA+GGG | - | Chr2:11691650-11691669 | None:intergenic | 35.0% |
| ! | TTGAGTAATTTGCATGTGAG+GGG | - | Chr2:11691649-11691668 | None:intergenic | 35.0% |
| ! | TTTAGGAGTTTCGTTTTCCA+GGG | - | Chr2:11691598-11691617 | None:intergenic | 35.0% |
| !! | TTTTAGGAGTTTCGTTTTCC+AGG | - | Chr2:11691599-11691618 | None:intergenic | 35.0% |
| GCAAATTACTCAAGCTGTCT+TGG | + | Chr2:11691656-11691675 | MsG0280007194.01.T01:CDS | 40.0% | |
| GCTGTCTTGGAGAAAAGTAA+TGG | + | Chr2:11691669-11691688 | MsG0280007194.01.T01:CDS | 40.0% | |
| GTCTTGGAGAAAAGTAATGG+TGG | + | Chr2:11691672-11691691 | MsG0280007194.01.T01:CDS | 40.0% | |
| TTGGAGAAAAGTAATGGTGG+TGG | + | Chr2:11691675-11691694 | MsG0280007194.01.T01:CDS | 40.0% | |
| ! | ACGGAATCATCAGGAGTTTT+AGG | - | Chr2:11691615-11691634 | None:intergenic | 40.0% |
| ! | AGACAGATTCTGTGGATTGA+TGG | + | Chr2:11691720-11691739 | MsG0280007194.01.T01:CDS | 40.0% |
| ! | GCTTGAGTAATTTGCATGTG+AGG | - | Chr2:11691651-11691670 | None:intergenic | 40.0% |
| AAAGGAAGCTCAGAAGCTGC+AGG | + | Chr2:11691773-11691792 | MsG0280007194.01.T01:CDS | 50.0% | |
| GCATCTAGCACGGAATCATC+AGG | - | Chr2:11691624-11691643 | None:intergenic | 50.0% | |
| GTGAGGGGATGCATCTAGCA+CGG | - | Chr2:11691634-11691653 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 11691582 | 11691803 | 11691582 | ID=MsG0280007194.01;Name=MsG0280007194.01 |
| Chr2 | mRNA | 11691582 | 11691803 | 11691582 | ID=MsG0280007194.01.T01;Parent=MsG0280007194.01;Name=MsG0280007194.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|73 |
| Chr2 | exon | 11691582 | 11691803 | 11691582 | ID=MsG0280007194.01.T01:exon:374;Parent=MsG0280007194.01.T01 |
| Chr2 | CDS | 11691582 | 11691803 | 11691582 | ID=MsG0280007194.01.T01:cds;Parent=MsG0280007194.01.T01 |
| Gene Sequence |
| Protein sequence |
