Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280007517.01 | MsG0280007522.01 | 0.910521 | 1.706443e-82 | 3.497925e-78 |
| MsG0280007518.01 | MsG0280007522.01 | 0.947749 | 3.664190e-106 | 6.515871e-101 |
| MsG0280007522.01 | MsG0280007524.01 | 0.849818 | 2.532405e-60 | 4.860760e-57 |
| MsG0280007522.01 | MsG0280008939.01 | 0.813119 | 3.007423e-51 | 1.946841e-48 |
| MsG0280007522.01 | MsG0280010569.01 | 0.815097 | 1.100705e-51 | 7.517772e-49 |
| MsG0280007522.01 | MsG0480023656.01 | 0.811964 | 5.380293e-51 | 3.377186e-48 |
| MsG0280007522.01 | MsG0580026144.01 | 0.803990 | 2.677874e-49 | 1.364991e-46 |
| MsG0280007522.01 | MsG0680030680.01 | 0.812120 | 4.974190e-51 | 3.135586e-48 |
| MsG0280007522.01 | MsG0780041262.01 | 0.817940 | 2.540800e-52 | 1.875948e-49 |
| MsG0280007522.01 | MsG0880044538.01 | 0.800804 | 1.213421e-48 | 5.702704e-46 |
| MsG0280007522.01 | MsG0880045573.01 | 0.819036 | 1.433721e-52 | 1.091141e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 50 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCAAATGTTCAGGTTTAAA+AGG | 0.204426 | 2:-16544690 | MsG0280007522.01.T01:CDS |
| GTGGAAGGAGGTGTGGTAGG+TGG | 0.274444 | 2:-16544595 | MsG0280007522.01.T01:CDS |
| TGGAAGGAGGTGTGGTAGGT+GGG | 0.286221 | 2:-16544594 | MsG0280007522.01.T01:CDS |
| GGAAGGAGGTGTGGTAGGTG+GGG | 0.297605 | 2:-16544593 | MsG0280007522.01.T01:CDS |
| ATACGTTGGCGCAAATGTTC+AGG | 0.359112 | 2:-16544699 | MsG0280007522.01.T01:CDS |
| GGTGGTGGTGTGGAAGGAGG+TGG | 0.386380 | 2:-16544481 | MsG0280007522.01.T01:CDS |
| GGTGCTGAAGGAGCTATAAT+AGG | 0.409389 | 2:-16544556 | MsG0280007522.01.T01:CDS |
| GAAGGAGGTGTGGTAGGTGG+GGG | 0.411184 | 2:-16544592 | MsG0280007522.01.T01:CDS |
| GGAGGTGTCGTAGGAATTGA+TGG | 0.412771 | 2:-16544622 | MsG0280007522.01.T01:CDS |
| GGTGTGGAAGGAGGTGTGGT+AGG | 0.444236 | 2:-16544598 | MsG0280007522.01.T01:CDS |
| GGAGTCGATGGTGGTGTAGA+AGG | 0.446829 | 2:-16544643 | MsG0280007522.01.T01:CDS |
| GTGGGTGGTGGTGTGGAAGG+AGG | 0.451417 | 2:-16544484 | MsG0280007522.01.T01:CDS |
| TGCACTCAGAGCTAATATCT+TGG | 0.474145 | 2:+16544813 | None:intergenic |
| GCACTCAGAGCTAATATCTT+GGG | 0.475433 | 2:+16544814 | None:intergenic |
| ATCACAGGTGGTGGTGTTGA+AGG | 0.478574 | 2:-16544445 | MsG0280007522.01.T01:CDS |
| GGAGCTATAATAGGTGGTGA+TGG | 0.499770 | 2:-16544547 | MsG0280007522.01.T01:CDS |
| ACTGTGGGTGGTGGTGTGGA+AGG | 0.518532 | 2:-16544487 | MsG0280007522.01.T01:CDS |
| GAAGGAGGTGTCGAAGGTGG+TGG | 0.519674 | 2:-16544523 | MsG0280007522.01.T01:CDS |
| GGTGTAGAAGGAGGTGTCGT+AGG | 0.524763 | 2:-16544631 | MsG0280007522.01.T01:CDS |
| TACAACAAGGATTTGTTCGT+GGG | 0.526682 | 2:-16544741 | MsG0280007522.01.T01:CDS |
| CCTTGTTGATCTAGATGCCT+CGG | 0.532285 | 2:+16544777 | None:intergenic |
| GTCGAAGGTGGAGGTGCTGA+AGG | 0.535205 | 2:-16544568 | MsG0280007522.01.T01:CDS |
| GGAATTGATGGTGGTGTGGA+AGG | 0.536327 | 2:-16544610 | MsG0280007522.01.T01:CDS |
| GTGGAAGGAGGTGGTGTCGA+AGG | 0.538378 | 2:-16544472 | MsG0280007522.01.T01:CDS |
| GTCGAAGGTGGTGGTGTCGA+AGG | 0.546498 | 2:-16544514 | MsG0280007522.01.T01:CDS |
| ATAATAGGTGGTGATGGAGA+AGG | 0.546891 | 2:-16544541 | MsG0280007522.01.T01:CDS |
| ATCAACTGTGGGTGGTGGTG+TGG | 0.547443 | 2:-16544491 | MsG0280007522.01.T01:CDS |
| TGGTGGTGTGGAAGGAGGTG+TGG | 0.549904 | 2:-16544602 | MsG0280007522.01.T01:CDS |
| CTACAACAAGGATTTGTTCG+TGG | 0.565019 | 2:-16544742 | MsG0280007522.01.T01:CDS |
| GATGGAGAAGGAGGTGTCGA+AGG | 0.568848 | 2:-16544529 | MsG0280007522.01.T01:CDS |
| ATAGGTGGTGATGGAGAAGG+AGG | 0.573916 | 2:-16544538 | MsG0280007522.01.T01:CDS |
| ATTGATGGTGGTGTGGAAGG+AGG | 0.574469 | 2:-16544607 | MsG0280007522.01.T01:CDS |
| GAAGGAGTAATCACAGGTGG+TGG | 0.586907 | 2:-16544454 | MsG0280007522.01.T01:CDS |
| GAAGGATCAACTGTGGGTGG+TGG | 0.590571 | 2:-16544496 | MsG0280007522.01.T01:CDS |
| GGAGAAGGAGGTGTCGAAGG+TGG | 0.595365 | 2:-16544526 | MsG0280007522.01.T01:CDS |
| GATCAACAAGGCCACTGATG+AGG | 0.608971 | 2:-16544765 | MsG0280007522.01.T01:CDS |
| CGTAGGAATTGATGGTGGTG+TGG | 0.614401 | 2:-16544614 | MsG0280007522.01.T01:CDS |
| GGTGTCGTTGGAGTCGATGG+TGG | 0.627154 | 2:-16544652 | MsG0280007522.01.T01:CDS |
| GGTGTCGAAGGAGTAATCAC+AGG | 0.627685 | 2:-16544460 | MsG0280007522.01.T01:CDS |
| GGTGTCGAAGGATCAACTGT+GGG | 0.629264 | 2:-16544502 | MsG0280007522.01.T01:CDS |
| CCTTGTTGTAGCCTCATCAG+TGG | 0.641265 | 2:+16544754 | None:intergenic |
| GTCGAAGGATCAACTGTGGG+TGG | 0.642906 | 2:-16544499 | MsG0280007522.01.T01:CDS |
| GTCGATGGTGGTGTAGAAGG+AGG | 0.650665 | 2:-16544640 | MsG0280007522.01.T01:CDS |
| GCTGAAGGAGCTATAATAGG+TGG | 0.664529 | 2:-16544553 | MsG0280007522.01.T01:CDS |
| GTCGAAGGAGTAATCACAGG+TGG | 0.669993 | 2:-16544457 | MsG0280007522.01.T01:CDS |
| CCACTGATGAGGCTACAACA+AGG | 0.671797 | 2:-16544754 | MsG0280007522.01.T01:CDS |
| CCGAGGCATCTAGATCAACA+AGG | 0.679099 | 2:-16544777 | MsG0280007522.01.T01:CDS |
| TGGTGTCGAAGGATCAACTG+TGG | 0.694920 | 2:-16544503 | MsG0280007522.01.T01:CDS |
| GGTGTCGTAGGAATTGATGG+TGG | 0.714173 | 2:-16544619 | MsG0280007522.01.T01:CDS |
| TGCAGCACAAAGACACACCG+AGG | 0.744286 | 2:-16544794 | MsG0280007522.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAACTATATTCAATACGT+TGG | - | Chr2:16544554-16544573 | MsG0280007522.01.T01:CDS | 20.0% |
| !!! | TTTAAAAGGTTACAATTTTG+CGG | - | Chr2:16544591-16544610 | MsG0280007522.01.T01:CDS | 20.0% |
| !! | TTAAAAGGTTACAATTTTGC+GGG | - | Chr2:16544592-16544611 | MsG0280007522.01.T01:CDS | 25.0% |
| ! | TAAAAGGTTACAATTTTGCG+GGG | - | Chr2:16544593-16544612 | MsG0280007522.01.T01:CDS | 30.0% |
| ! | AAAAGGTTACAATTTTGCGG+GGG | - | Chr2:16544594-16544613 | MsG0280007522.01.T01:CDS | 35.0% |
| ! | CGCAAATGTTCAGGTTTAAA+AGG | - | Chr2:16544577-16544596 | MsG0280007522.01.T01:CDS | 35.0% |
| ! | TACAACAAGGATTTGTTCGT+GGG | - | Chr2:16544526-16544545 | MsG0280007522.01.T01:CDS | 35.0% |
| GCACTCAGAGCTAATATCTT+GGG | + | Chr2:16544456-16544475 | None:intergenic | 40.0% | |
| TGCACTCAGAGCTAATATCT+TGG | + | Chr2:16544457-16544476 | None:intergenic | 40.0% | |
| ! | CTACAACAAGGATTTGTTCG+TGG | - | Chr2:16544525-16544544 | MsG0280007522.01.T01:CDS | 40.0% |
| !! | ATAATAGGTGGTGATGGAGA+AGG | - | Chr2:16544726-16544745 | MsG0280007522.01.T01:CDS | 40.0% |
| ATACGTTGGCGCAAATGTTC+AGG | - | Chr2:16544568-16544587 | MsG0280007522.01.T01:CDS | 45.0% | |
| CCTTGTTGATCTAGATGCCT+CGG | + | Chr2:16544493-16544512 | None:intergenic | 45.0% | |
| GCTGAAGGAGCTATAATAGG+TGG | - | Chr2:16544714-16544733 | MsG0280007522.01.T01:CDS | 45.0% | |
| GGAGCTATAATAGGTGGTGA+TGG | - | Chr2:16544720-16544739 | MsG0280007522.01.T01:CDS | 45.0% | |
| ! | GGTGCTGAAGGAGCTATAAT+AGG | - | Chr2:16544711-16544730 | MsG0280007522.01.T01:CDS | 45.0% |
| CCACTGATGAGGCTACAACA+AGG | - | Chr2:16544513-16544532 | MsG0280007522.01.T01:CDS | 50.0% | |
| GAAGGAGTAATCACAGGTGG+TGG | - | Chr2:16544813-16544832 | MsG0280007522.01.T01:CDS | 50.0% | |
| GATCAACAAGGCCACTGATG+AGG | - | Chr2:16544502-16544521 | MsG0280007522.01.T01:CDS | 50.0% | |
| GTCGAAGGAGTAATCACAGG+TGG | - | Chr2:16544810-16544829 | MsG0280007522.01.T01:CDS | 50.0% | |
| ! | ATCACAGGTGGTGGTGTTGA+AGG | - | Chr2:16544822-16544841 | MsG0280007522.01.T01:CDS | 50.0% |
| ! | CCGAGGCATCTAGATCAACA+AGG | - | Chr2:16544490-16544509 | MsG0280007522.01.T01:CDS | 50.0% |
| ! | CCTTGTTGTAGCCTCATCAG+TGG | + | Chr2:16544516-16544535 | None:intergenic | 50.0% |
| ! | GGTGTCGAAGGAGTAATCAC+AGG | - | Chr2:16544807-16544826 | MsG0280007522.01.T01:CDS | 50.0% |
| ! | GGTGTCGAAGGATCAACTGT+GGG | - | Chr2:16544765-16544784 | MsG0280007522.01.T01:CDS | 50.0% |
| ! | GGTGTCGTAGGAATTGATGG+TGG | - | Chr2:16544648-16544667 | MsG0280007522.01.T01:CDS | 50.0% |
| ! | TGGTGTCGAAGGATCAACTG+TGG | - | Chr2:16544764-16544783 | MsG0280007522.01.T01:CDS | 50.0% |
| !! | ATAGGTGGTGATGGAGAAGG+AGG | - | Chr2:16544729-16544748 | MsG0280007522.01.T01:CDS | 50.0% |
| !! | ATTGATGGTGGTGTGGAAGG+AGG | - | Chr2:16544660-16544679 | MsG0280007522.01.T01:CDS | 50.0% |
| !! | CGTAGGAATTGATGGTGGTG+TGG | - | Chr2:16544653-16544672 | MsG0280007522.01.T01:CDS | 50.0% |
| !! | GGAATTGATGGTGGTGTGGA+AGG | - | Chr2:16544657-16544676 | MsG0280007522.01.T01:CDS | 50.0% |
| !! | GGAGGTGTCGTAGGAATTGA+TGG | - | Chr2:16544645-16544664 | MsG0280007522.01.T01:CDS | 50.0% |
| GAAGGATCAACTGTGGGTGG+TGG | - | Chr2:16544771-16544790 | MsG0280007522.01.T01:CDS | 55.0% | |
| GTCGAAGGATCAACTGTGGG+TGG | - | Chr2:16544768-16544787 | MsG0280007522.01.T01:CDS | 55.0% | |
| TGCAGCACAAAGACACACCG+AGG | - | Chr2:16544473-16544492 | MsG0280007522.01.T01:CDS | 55.0% | |
| ! | ATCAACTGTGGGTGGTGGTG+TGG | - | Chr2:16544776-16544795 | MsG0280007522.01.T01:CDS | 55.0% |
| ! | GGAGTCGATGGTGGTGTAGA+AGG | - | Chr2:16544624-16544643 | MsG0280007522.01.T01:CDS | 55.0% |
| ! | GTCGATGGTGGTGTAGAAGG+AGG | - | Chr2:16544627-16544646 | MsG0280007522.01.T01:CDS | 55.0% |
| ! | TGGAAGGAGGTGTGGTAGGT+GGG | - | Chr2:16544673-16544692 | MsG0280007522.01.T01:CDS | 55.0% |
| !! | GATGGAGAAGGAGGTGTCGA+AGG | - | Chr2:16544738-16544757 | MsG0280007522.01.T01:CDS | 55.0% |
| !! | GGTGTAGAAGGAGGTGTCGT+AGG | - | Chr2:16544636-16544655 | MsG0280007522.01.T01:CDS | 55.0% |
| !!! | CAATTTTGCGGGGGTGTCGT+TGG | - | Chr2:16544603-16544622 | MsG0280007522.01.T01:CDS | 55.0% |
| ! | ACTGTGGGTGGTGGTGTGGA+AGG | - | Chr2:16544780-16544799 | MsG0280007522.01.T01:CDS | 60.0% |
| ! | GAAGGAGGTGTGGTAGGTGG+GGG | - | Chr2:16544675-16544694 | MsG0280007522.01.T01:CDS | 60.0% |
| ! | GGAAGGAGGTGTGGTAGGTG+GGG | - | Chr2:16544674-16544693 | MsG0280007522.01.T01:CDS | 60.0% |
| ! | GGTGTGGAAGGAGGTGTGGT+AGG | - | Chr2:16544669-16544688 | MsG0280007522.01.T01:CDS | 60.0% |
| ! | GTCGAAGGTGGTGGTGTCGA+AGG | - | Chr2:16544753-16544772 | MsG0280007522.01.T01:CDS | 60.0% |
| ! | GTGGAAGGAGGTGGTGTCGA+AGG | - | Chr2:16544795-16544814 | MsG0280007522.01.T01:CDS | 60.0% |
| ! | GTGGAAGGAGGTGTGGTAGG+TGG | - | Chr2:16544672-16544691 | MsG0280007522.01.T01:CDS | 60.0% |
| ! | TGGTGGTGTGGAAGGAGGTG+TGG | - | Chr2:16544665-16544684 | MsG0280007522.01.T01:CDS | 60.0% |
| !! | GAAGGAGGTGTCGAAGGTGG+TGG | - | Chr2:16544744-16544763 | MsG0280007522.01.T01:CDS | 60.0% |
| !! | GGAGAAGGAGGTGTCGAAGG+TGG | - | Chr2:16544741-16544760 | MsG0280007522.01.T01:CDS | 60.0% |
| !! | GGTGTCGTTGGAGTCGATGG+TGG | - | Chr2:16544615-16544634 | MsG0280007522.01.T01:CDS | 60.0% |
| !! | GTCGAAGGTGGAGGTGCTGA+AGG | - | Chr2:16544699-16544718 | MsG0280007522.01.T01:CDS | 60.0% |
| ! | GGTGGTGGTGTGGAAGGAGG+TGG | - | Chr2:16544786-16544805 | MsG0280007522.01.T01:CDS | 65.0% |
| ! | GTGGGTGGTGGTGTGGAAGG+AGG | - | Chr2:16544783-16544802 | MsG0280007522.01.T01:CDS | 65.0% |
| !! | GGGGGTGTCGTTGGAGTCGA+TGG | - | Chr2:16544612-16544631 | MsG0280007522.01.T01:CDS | 65.0% |
| !! | GTAGGTGGGGGTGTCGAAGG+TGG | - | Chr2:16544687-16544706 | MsG0280007522.01.T01:CDS | 65.0% |
| !! | GTGGTAGGTGGGGGTGTCGA+AGG | - | Chr2:16544684-16544703 | MsG0280007522.01.T01:CDS | 65.0% |
| !! | GGTGGGGGTGTCGAAGGTGG+AGG | - | Chr2:16544690-16544709 | MsG0280007522.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 16544438 | 16544851 | 16544438 | ID=MsG0280007522.01;Name=MsG0280007522.01 |
| Chr2 | mRNA | 16544438 | 16544851 | 16544438 | ID=MsG0280007522.01.T01;Parent=MsG0280007522.01;Name=MsG0280007522.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|137 |
| Chr2 | exon | 16544438 | 16544851 | 16544438 | ID=MsG0280007522.01.T01:exon:10155;Parent=MsG0280007522.01.T01 |
| Chr2 | CDS | 16544438 | 16544851 | 16544438 | ID=MsG0280007522.01.T01:cds;Parent=MsG0280007522.01.T01 |
| Gene Sequence |
| Protein sequence |
