AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580026144.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026144.01.T01 MTR_5g040340 91.932 471 25 3 1 464 1 465 0.0 864
MsG0580026144.01.T01 MTR_8g055860 47.036 506 167 24 17 464 29 491 5.13e-103 316
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026144.01.T01 AT2G02950 37.081 418 157 21 55 439 76 420 4.75e-48 171
MsG0580026144.01.T01 AT1G14280 34.353 425 167 23 52 435 63 416 5.28e-45 163

Find 70 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGCATCTCTTTATTGATTT+TGG 0.002063 5:+31329724 None:intergenic
AAAGAAGAAGATGGAGAAAT+TGG 0.158218 5:-31330700 MsG0580026144.01.T01:CDS
GAATTAAACGATGTTCTTAT+TGG 0.158853 5:+31329761 None:intergenic
GCCTCAGATGACAAGGTTTA+AGG 0.196735 5:-31329591 MsG0580026144.01.T01:CDS
TTCCTTTCTATTGCTGATAA+AGG 0.219072 5:+31330744 None:intergenic
TGCACTTTCTATTCTCTTGT+TGG 0.253513 5:+31330584 None:intergenic
TTGCATCTTTCATCTAATAT+TGG 0.258454 5:+31330145 None:intergenic
ACATCTAAGGTAGCAATTCT+TGG 0.295124 5:+31330631 None:intergenic
TCCTTTCTATTGCTGATAAA+GGG 0.308318 5:+31330745 None:intergenic
CTTGAGTTTATTGACATCTA+AGG 0.320092 5:+31330618 None:intergenic
CAGAAAGCTGTTGGTGTTGC+TGG 0.351018 5:-31329665 MsG0580026144.01.T01:CDS
AGGTTTAAGGTGGAAAGTTT+TGG 0.354957 5:-31329578 MsG0580026144.01.T01:CDS
AGGAAAACATTGGAAGTGTT+TGG 0.359214 5:-31330172 MsG0580026144.01.T01:CDS
AGAAAGTGCAAAGTTCAATA+TGG 0.368278 5:-31330571 MsG0580026144.01.T01:CDS
TCAACATCACATGTTGCTTC+TGG 0.368292 5:-31329914 MsG0580026144.01.T01:CDS
CATTCAATGCTTGCTTCACT+TGG 0.371898 5:+31329866 None:intergenic
AGAAATGAACAACATGGTTA+TGG 0.378020 5:-31329551 MsG0580026144.01.T01:CDS
GTTATGGAAAACCTCCTCTT+AGG 0.383306 5:-31329535 MsG0580026144.01.T01:CDS
AAGAGTCTTCTTGCTACTCT+TGG 0.400812 5:-31330439 MsG0580026144.01.T01:CDS
AGTCAATAAGCTTTGATATG+AGG 0.407049 5:-31330123 MsG0580026144.01.T01:CDS
TTCAACAAAAGTGCAGTTCA+TGG 0.420124 5:-31330352 MsG0580026144.01.T01:CDS
TGAGGCTTAAAATACCATCT+TGG 0.433293 5:-31330105 MsG0580026144.01.T01:CDS
GAGGCTTAAAATACCATCTT+GGG 0.434991 5:-31330104 MsG0580026144.01.T01:CDS
CTTGATTCTGAGCCAACACT+TGG 0.447581 5:+31330544 None:intergenic
CGGCGTCATGGAAAATTGTT+GGG 0.449202 5:-31329698 MsG0580026144.01.T01:CDS
CTTCTTCTTTCTTTACTCCA+AGG 0.457657 5:+31330713 None:intergenic
GCGGCGTCATGGAAAATTGT+TGG 0.460904 5:-31329699 MsG0580026144.01.T01:CDS
AAGGTTCAGTAGTAACTATA+AGG 0.462753 5:-31329787 MsG0580026144.01.T01:CDS
TTTGAGATTGAAAGCTTGAC+TGG 0.473135 5:-31329968 MsG0580026144.01.T01:CDS
GAAGACTCTTTCTATGAACT+TGG 0.474532 5:+31330452 None:intergenic
TTCGAAGCAGCTTCCCAAGA+TGG 0.484347 5:+31330091 None:intergenic
CTTGAACAACAAGGAAGTAA+AGG 0.492569 5:-31330791 MsG0580026144.01.T01:CDS
TTCCGCTGCAGCACGAGTCT+CGG 0.494266 5:+31330258 None:intergenic
GTTTCAGATCATGAAGATCA+AGG 0.495758 5:-31329806 MsG0580026144.01.T01:CDS
CCCCTTTATCAGCAATAGAA+AGG 0.507913 5:-31330746 MsG0580026144.01.T01:CDS
CAAGTGAAGCAAGCATTGAA+TGG 0.508457 5:-31329865 MsG0580026144.01.T01:CDS
GGGTGGATTGAATCAGCAAA+AGG 0.523505 5:+31330766 None:intergenic
TTCTATTGCTGATAAAGGGG+TGG 0.524721 5:+31330749 None:intergenic
ACATCGTTTAATTCATCGAA+TGG 0.528036 5:-31329752 MsG0580026144.01.T01:CDS
TTCTTTACTCCAAGGTGAAG+AGG 0.529025 5:+31330721 None:intergenic
CCTTTCTATTGCTGATAAAG+GGG 0.530407 5:+31330746 None:intergenic
GGAGTAAAGAAAGAAGAAGA+TGG 0.538350 5:-31330709 MsG0580026144.01.T01:CDS
ACCTTAAACCTTGTCATCTG+AGG 0.538940 5:+31329590 None:intergenic
GAGATGCCGAGGCGGCGTCA+TGG 0.540133 5:-31329710 MsG0580026144.01.T01:CDS
AACCGAGACTCGTGCTGCAG+CGG 0.552008 5:-31330260 MsG0580026144.01.T01:CDS
AGATAATCCAAGGAAAACAT+TGG 0.565125 5:-31330182 MsG0580026144.01.T01:CDS
GGTGAATTTGATCTCCTAAG+AGG 0.568136 5:+31329521 None:intergenic
GTTATTCTGAGAGGTGAAAG+TGG 0.569101 5:+31330849 None:intergenic
CAGTTTGTAAAAGTGCACTT+TGG 0.569109 5:+31330512 None:intergenic
TTCAACAAGTAGATAATCCA+AGG 0.572162 5:-31330192 MsG0580026144.01.T01:CDS
TGTAATAGTCAGAAAGCTGT+TGG 0.572244 5:-31329674 MsG0580026144.01.T01:CDS
GTTATTGTTGTTATTCTGAG+AGG 0.577463 5:+31330840 None:intergenic
CAATATGGAACTCCAAGTGT+TGG 0.581850 5:-31330556 MsG0580026144.01.T01:CDS
AGAAAGGAACCTCTTCACCT+TGG 0.586793 5:-31330730 MsG0580026144.01.T01:CDS
GAATTTGATCTCCTAAGAGG+AGG 0.592977 5:+31329524 None:intergenic
CTCTTCATACTTGAACAACA+AGG 0.600465 5:-31330800 MsG0580026144.01.T01:CDS
AAATGTTGCCTCAGATGACA+AGG 0.605171 5:-31329598 MsG0580026144.01.T01:CDS
TCAATAAAGAGATGCCGAGG+CGG 0.619222 5:-31329718 MsG0580026144.01.T01:CDS
TCAGATGACAAGGTTTAAGG+TGG 0.630245 5:-31329588 MsG0580026144.01.T01:CDS
GGTGCAAGAAATGAACAACA+TGG 0.655080 5:-31329557 MsG0580026144.01.T01:CDS
AAATCAATAAAGAGATGCCG+AGG 0.660495 5:-31329721 MsG0580026144.01.T01:CDS
AACAACAATAACCTCCGTGG+TGG 0.687252 5:-31330829 MsG0580026144.01.T01:CDS
GTTGATGTAAGTGATCATGC+AGG 0.688347 5:-31330385 MsG0580026144.01.T01:CDS
AGGAGATCAAATTCACCACA+TGG 0.692006 5:-31329515 MsG0580026144.01.T01:CDS
AGCAAGCATTGAATGGAGTG+TGG 0.726329 5:-31329858 MsG0580026144.01.T01:CDS
ATGAAGAGAAAGAACCACCA+CGG 0.734637 5:+31330815 None:intergenic
TACAAAAGTTGTGCACCATG+TGG 0.736641 5:+31329500 None:intergenic
AATAACAACAATAACCTCCG+TGG 0.749739 5:-31330832 MsG0580026144.01.T01:CDS
AGTGGTTGCTGAGATAACCA+TGG 0.758373 5:+31330867 None:intergenic
AAGAGAAAGAACCACCACGG+AGG 0.793634 5:+31330818 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GAATTAAACGATGTTCTTAT+TGG + Chr5:31330600-31330619 None:intergenic 25.0%
! TGTTCTTGATAAAAATGAGT+TGG + Chr5:31330424-31330443 None:intergenic 25.0%
! TTGCATCTTTCATCTAATAT+TGG + Chr5:31330216-31330235 None:intergenic 25.0%
AAAGAAGAAGATGGAGAAAT+TGG - Chr5:31329658-31329677 MsG0580026144.01.T01:CDS 30.0%
AAGGTTCAGTAGTAACTATA+AGG - Chr5:31330571-31330590 MsG0580026144.01.T01:CDS 30.0%
ACATCGTTTAATTCATCGAA+TGG - Chr5:31330606-31330625 MsG0580026144.01.T01:CDS 30.0%
AGAAATGAACAACATGGTTA+TGG - Chr5:31330807-31330826 MsG0580026144.01.T01:CDS 30.0%
AGATAATCCAAGGAAAACAT+TGG - Chr5:31330176-31330195 MsG0580026144.01.T01:CDS 30.0%
CTTGAGTTTATTGACATCTA+AGG + Chr5:31329743-31329762 None:intergenic 30.0%
GATGCTGAAAAATACTTCAA+TGG - Chr5:31329688-31329707 MsG0580026144.01.T01:CDS 30.0%
TCCTTTCTATTGCTGATAAA+GGG + Chr5:31329616-31329635 None:intergenic 30.0%
TTCAACAAGTAGATAATCCA+AGG - Chr5:31330166-31330185 MsG0580026144.01.T01:CDS 30.0%
TTCCTTTCTATTGCTGATAA+AGG + Chr5:31329617-31329636 None:intergenic 30.0%
! AGAAAGTGCAAAGTTCAATA+TGG - Chr5:31329787-31329806 MsG0580026144.01.T01:CDS 30.0%
! AGTCAATAAGCTTTGATATG+AGG - Chr5:31330235-31330254 MsG0580026144.01.T01:CDS 30.0%
! ATGTTTGAAGAGTTTTTCTG+AGG + Chr5:31330040-31330059 None:intergenic 30.0%
! GATAATGATGATGATGATGT+TGG - Chr5:31330345-31330364 MsG0580026144.01.T01:CDS 30.0%
! GTTATTGTTGTTATTCTGAG+AGG + Chr5:31329521-31329540 None:intergenic 30.0%
!! TCACTTGGTGCATAAAAATT+TGG + Chr5:31330480-31330499 None:intergenic 30.0%
!!! GGCATCTCTTTATTGATTTT+GGG + Chr5:31330636-31330655 None:intergenic 30.0%
AAATCAATAAAGAGATGCCG+AGG - Chr5:31330637-31330656 MsG0580026144.01.T01:CDS 35.0%
AATAACAACAATAACCTCCG+TGG - Chr5:31329526-31329545 MsG0580026144.01.T01:CDS 35.0%
CCTTTCTATTGCTGATAAAG+GGG + Chr5:31329615-31329634 None:intergenic 35.0%
CTCTTCATACTTGAACAACA+AGG - Chr5:31329558-31329577 MsG0580026144.01.T01:CDS 35.0%
CTTCTTCTTTCTTTACTCCA+AGG + Chr5:31329648-31329667 None:intergenic 35.0%
CTTGAACAACAAGGAAGTAA+AGG - Chr5:31329567-31329586 MsG0580026144.01.T01:CDS 35.0%
GAAGACTCTTTCTATGAACT+TGG + Chr5:31329909-31329928 None:intergenic 35.0%
GAGGCTTAAAATACCATCTT+GGG - Chr5:31330254-31330273 MsG0580026144.01.T01:CDS 35.0%
GGAGTAAAGAAAGAAGAAGA+TGG - Chr5:31329649-31329668 MsG0580026144.01.T01:CDS 35.0%
GTTTCAGATCATGAAGATCA+AGG - Chr5:31330552-31330571 MsG0580026144.01.T01:CDS 35.0%
TGAGGCTTAAAATACCATCT+TGG - Chr5:31330253-31330272 MsG0580026144.01.T01:CDS 35.0%
TGCACTTTCTATTCTCTTGT+TGG + Chr5:31329777-31329796 None:intergenic 35.0%
TGTAATAGTCAGAAAGCTGT+TGG - Chr5:31330684-31330703 MsG0580026144.01.T01:CDS 35.0%
! AACACTTCCAATGTTTTCCT+TGG + Chr5:31330186-31330205 None:intergenic 35.0%
! ACATCTAAGGTAGCAATTCT+TGG + Chr5:31329730-31329749 None:intergenic 35.0%
! CAGTTTGTAAAAGTGCACTT+TGG + Chr5:31329849-31329868 None:intergenic 35.0%
! TTCAACAAAAGTGCAGTTCA+TGG - Chr5:31330006-31330025 MsG0580026144.01.T01:CDS 35.0%
! TTTGAGATTGAAAGCTTGAC+TGG - Chr5:31330390-31330409 MsG0580026144.01.T01:CDS 35.0%
!! AGGAAAACATTGGAAGTGTT+TGG - Chr5:31330186-31330205 MsG0580026144.01.T01:CDS 35.0%
!! AGGTTTAAGGTGGAAAGTTT+TGG - Chr5:31330780-31330799 MsG0580026144.01.T01:CDS 35.0%
!!! CGGCATCTCTTTATTGATTT+TGG + Chr5:31330637-31330656 None:intergenic 35.0%
AAATGTTGCCTCAGATGACA+AGG - Chr5:31330760-31330779 MsG0580026144.01.T01:CDS 40.0%
AAGAGTCTTCTTGCTACTCT+TGG - Chr5:31329919-31329938 MsG0580026144.01.T01:CDS 40.0%
ACCTTAAACCTTGTCATCTG+AGG + Chr5:31330771-31330790 None:intergenic 40.0%
AGGAGATCAAATTCACCACA+TGG - Chr5:31330843-31330862 MsG0580026144.01.T01:CDS 40.0%
ATGAAGAGAAAGAACCACCA+CGG + Chr5:31329546-31329565 None:intergenic 40.0%
CAATATGGAACTCCAAGTGT+TGG - Chr5:31329802-31329821 MsG0580026144.01.T01:CDS 40.0%
CATTCAATGCTTGCTTCACT+TGG + Chr5:31330495-31330514 None:intergenic 40.0%
CCCCTTTATCAGCAATAGAA+AGG - Chr5:31329612-31329631 MsG0580026144.01.T01:CDS 40.0%
GAATTTGATCTCCTAAGAGG+AGG + Chr5:31330837-31330856 None:intergenic 40.0%
GGTGAATTTGATCTCCTAAG+AGG + Chr5:31330840-31330859 None:intergenic 40.0%
GGTGCAAGAAATGAACAACA+TGG - Chr5:31330801-31330820 MsG0580026144.01.T01:CDS 40.0%
GTTATGGAAAACCTCCTCTT+AGG - Chr5:31330823-31330842 MsG0580026144.01.T01:CDS 40.0%
GTTGATGTAAGTGATCATGC+AGG - Chr5:31329973-31329992 MsG0580026144.01.T01:CDS 40.0%
TACAAAAGTTGTGCACCATG+TGG + Chr5:31330861-31330880 None:intergenic 40.0%
TCAGATGACAAGGTTTAAGG+TGG - Chr5:31330770-31330789 MsG0580026144.01.T01:CDS 40.0%
TTCTATTGCTGATAAAGGGG+TGG + Chr5:31329612-31329631 None:intergenic 40.0%
TTCTTTACTCCAAGGTGAAG+AGG + Chr5:31329640-31329659 None:intergenic 40.0%
! GTTATTCTGAGAGGTGAAAG+TGG + Chr5:31329512-31329531 None:intergenic 40.0%
! TCAACATCACATGTTGCTTC+TGG - Chr5:31330444-31330463 MsG0580026144.01.T01:CDS 40.0%
!! CAAGTGAAGCAAGCATTGAA+TGG - Chr5:31330493-31330512 MsG0580026144.01.T01:CDS 40.0%
!! GTCATCTGAGGCAACATTTT+TGG + Chr5:31330759-31330778 None:intergenic 40.0%
AACAACAATAACCTCCGTGG+TGG - Chr5:31329529-31329548 MsG0580026144.01.T01:CDS 45.0%
AGAAAGGAACCTCTTCACCT+TGG - Chr5:31329628-31329647 MsG0580026144.01.T01:CDS 45.0%
CGGCGTCATGGAAAATTGTT+GGG - Chr5:31330660-31330679 MsG0580026144.01.T01:CDS 45.0%
CTTGATTCTGAGCCAACACT+TGG + Chr5:31329817-31329836 None:intergenic 45.0%
GCCTCAGATGACAAGGTTTA+AGG - Chr5:31330767-31330786 MsG0580026144.01.T01:CDS 45.0%
TCAATAAAGAGATGCCGAGG+CGG - Chr5:31330640-31330659 MsG0580026144.01.T01:CDS 45.0%
!! AGCAAGCATTGAATGGAGTG+TGG - Chr5:31330500-31330519 MsG0580026144.01.T01:CDS 45.0%
!! GGGTGGATTGAATCAGCAAA+AGG + Chr5:31329595-31329614 None:intergenic 45.0%
AAGAGAAAGAACCACCACGG+AGG + Chr5:31329543-31329562 None:intergenic 50.0%
GCGGCGTCATGGAAAATTGT+TGG - Chr5:31330659-31330678 MsG0580026144.01.T01:CDS 50.0%
TTCGAAGCAGCTTCCCAAGA+TGG + Chr5:31330270-31330289 None:intergenic 50.0%
! AATTTTCCATGACGCCGCCT+CGG + Chr5:31330657-31330676 None:intergenic 50.0%
!! CAGAAAGCTGTTGGTGTTGC+TGG - Chr5:31330693-31330712 MsG0580026144.01.T01:CDS 50.0%
AACCGAGACTCGTGCTGCAG+CGG - Chr5:31330098-31330117 MsG0580026144.01.T01:CDS 60.0%
TTCCGCTGCAGCACGAGTCT+CGG + Chr5:31330103-31330122 None:intergenic 60.0%
GAGATGCCGAGGCGGCGTCA+TGG - Chr5:31330648-31330667 MsG0580026144.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 31329493 31330887 31329493 ID=MsG0580026144.01;Name=MsG0580026144.01
Chr5 mRNA 31329493 31330887 31329493 ID=MsG0580026144.01.T01;Parent=MsG0580026144.01;Name=MsG0580026144.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|464
Chr5 exon 31329493 31330887 31329493 ID=MsG0580026144.01.T01:exon:13075;Parent=MsG0580026144.01.T01
Chr5 CDS 31329493 31330887 31329493 ID=MsG0580026144.01.T01:cds;Parent=MsG0580026144.01.T01
Gene Sequence

>MsG0580026144.01.T01

ATGGTTATCTCAGCAACCACTTTCACCTCTCAGAATAACAACAATAACCTCCGTGGTGGTTCTTTCTCTTCATACTTGAACAACAAGGAAGTAAAGGATCCTTTTGCTGATTCAATCCACCCCTTTATCAGCAATAGAAAGGAACCTCTTCACCTTGGAGTAAAGAAAGAAGAAGATGGAGAAATTGGAGTATTTGATGCTGAAAAATACTTCAATGGAGTTGAAATAGAAACTCCAAGAATTGCTACCTTAGATGTCAATAAACTCAAGTACTTGAAAGACCAACAAGAGAATAGAAAGTGCAAAGTTCAATATGGAACTCCAAGTGTTGGCTCAGAATCAAGCTTGAATAGCCAAAGTGCACTTTTACAAACTGCTTTGAAAAATTCTTCTTCAAGTAATAGTAGAAAAAACCAAGTTCATAGAAAGAGTCTTCTTGCTACTCTTGGTTGCAAATGTTCTTGTTATGATAAGAATTCTGTTGATGTAAGTGATCATGCAGGTGAAATCAGCTTCAACAAAAGTGCAGTTCATGGAAAAACAACCTCAGAAAAACTCTTCAAACATGATCAAGATGCTTCTGATCATTCAATTAAGAATAAGAAAACCGAGACTCGTGCTGCAGCGGAACTCTTGATGAACAAAGAAAAGATTTCTACTTACAAAATGCAACTTCAACAAGTAGATAATCCAAGGAAAACATTGGAAGTGTTTGGTTCGCCAATATTAGATGAAAGATGCAAGTCAATAAGCTTTGATATGAGGCTTAAAATACCATCTTGGGAAGCTGCTTCGAAACTTGAAGAAGAAGAAGAAATTGATTTTTCTACAAATTGTGATGAAAACAACTATGATAATGATGATGATGATGTTGGAAGTGATGCAAGTTCAGATTTATTTGAGATTGAAAGCTTGACTGGTAATAAAACCAACTCATTTTTATCAAGAACATCAACATCACATGTTGCTTCTGGTTGTACTAGTCCAAATTTTTATGCACCAAGTGAAGCAAGCATTGAATGGAGTGTGGTAACTGCAAGTGCAGTTGATTATTCAGCTGTTTCAGATCATGAAGATCAAGGTTCAGTAGTAACTATAAGGAGTCCAATAAGAACATCGTTTAATTCATCGAATGGTAAACCCAAAATCAATAAAGAGATGCCGAGGCGGCGTCATGGAAAATTGTTGGGTTGTAATAGTCAGAAAGCTGTTGGTGTTGCTGGTGATGCTTTCACAACAACAAATGAGAAACATAGTACAAATCCAAAAATGTTGCCTCAGATGACAAGGTTTAAGGTGGAAAGTTTTGGTGCAAGAAATGAACAACATGGTTATGGAAAACCTCCTCTTAGGAGATCAAATTCACCACATGGTGCACAACTTTTGTATATTTAG

Protein sequence

>MsG0580026144.01.T01

MVISATTFTSQNNNNNLRGGSFSSYLNNKEVKDPFADSIHPFISNRKEPLHLGVKKEEDGEIGVFDAEKYFNGVEIETPRIATLDVNKLKYLKDQQENRKCKVQYGTPSVGSESSLNSQSALLQTALKNSSSSNSRKNQVHRKSLLATLGCKCSCYDKNSVDVSDHAGEISFNKSAVHGKTTSEKLFKHDQDASDHSIKNKKTETRAAAELLMNKEKISTYKMQLQQVDNPRKTLEVFGSPILDERCKSISFDMRLKIPSWEAASKLEEEEEIDFSTNCDENNYDNDDDDVGSDASSDLFEIESLTGNKTNSFLSRTSTSHVASGCTSPNFYAPSEASIEWSVVTASAVDYSAVSDHEDQGSVVTIRSPIRTSFNSSNGKPKINKEMPRRRHGKLLGCNSQKAVGVAGDAFTTTNEKHSTNPKMLPQMTRFKVESFGARNEQHGYGKPPLRRSNSPHGAQLLYI*