AlfalfaGEDB | MsG0280007654.01

M. sativa cultivar ZhongmuNo.1 / MsG0280007654.01

KEGG: K02994
GO: GO:0003735 GO:0005840 GO:0006412
PFAM: PF00410

Nr:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007654.01.T01	YP_010173459.1	99.206	126	1	1	126	10	135	1.22E-83	251
MsG0280007654.01.T02	YP_010173459.1	99.145	117	1	1	117	19	135	7.63E-77	234
MsG0280007654.01.T03	QJD21000.1	96.875	32	1	1	32	86	117	2.97E-12	66.2

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007654.01.T01	Q2PMQ0	88.889	126	14	1	126	9	134	2.44E-77	228
MsG0280007654.01.T02	Q2PMQ0	90.598	117	11	1	117	18	134	1.37E-72	216
MsG0280007654.01.T03	Q2PMQ0	96.875	32	1	1	32	103	134	9.50E-15	65.9

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007654.01.T01	A0A898NA01	99.206	126	1	1	126	10	135	5.81e-84	251
MsG0280007654.01.T02	A0A898NA01	99.145	117	1	1	117	19	135	3.65e-77	234
MsG0280007654.01.T03	A0A6M3REF7	96.875	32	1	1	32	86	117	1.42e-12	66.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080049078.01	MsG0280007654.01	0.803684	3.099794e-49	1.567560e-46
MsG0280007654.01	MsG0380013955.01	0.825632	4.216893e-54	3.864974e-51
MsG0280007654.01	MsG0380013970.01	0.829660	4.547248e-55	4.684387e-52
MsG0280007654.01	MsG0380014007.01	0.820829	5.578214e-53	4.461995e-50
MsG0280007654.01	MsG0380014013.01	0.802658	5.053518e-49	2.489316e-46
MsG0280007654.01	MsG0380014022.01	0.812263	4.629242e-51	2.929623e-48
MsG0280007654.01	MsG0380014037.01	0.807677	4.497168e-50	2.520429e-47
MsG0280007654.01	MsG0380014038.01	0.812035	5.190385e-51	3.264190e-48
MsG0280007654.01	MsG0780037705.01	0.821336	4.262561e-53	3.458052e-50

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007654.01.T01	MTR_4g034810	99.153	118	1	0	1	118	1	118	2.67e-79	231
MsG0280007654.01.T01	MTR_4g059910	80.142	141	3	1	10	125	1	141	2.23e-72	213
MsG0280007654.01.T01	MTR_3g035490	98.077	52	1	0	35	86	150	201	6.84e-29	105
MsG0280007654.01.T02	MTR_4g034810	99.083	109	1	0	1	109	10	118	2.34e-72	213
MsG0280007654.01.T02	MTR_4g059910	80.142	141	3	1	1	116	1	141	2.61e-72	213
MsG0280007654.01.T02	MTR_3g035490	98.077	52	1	0	26	77	150	201	6.61e-29	105
MsG0280007654.01.T03	MTR_4g059910	100.000	31	0	0	1	31	111	141	1.91e-15	65.1

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007654.01.T01	ATCG00770	80.952	126	24	1	126	9	134	1.64e-71	211
MsG0280007654.01.T02	ATCG00770	82.051	117	21	1	117	18	134	9.95e-67	198
MsG0280007654.01.T03	ATCG00770	84.375	32	5	1	32	103	134	1.11e-13	60.5

Find 11 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGTAATTCTTTCTACTTCT+AGG	0.233849	2:+18604917	MsG0280007654.01.T01:CDS
TGTAATTCTTTCTACTTCTA+GGG	0.394272	2:+18604918	MsG0280007654.01.T01:CDS
CTCTACGGTATAGAAGAAAT+AGG	0.398659	2:+18604788	MsG0280007654.01.T01:CDS
ATAGAAATCAAATCGGATAT+CGG	0.428347	2:-18605016	None:intergenic
CCCATCCCTCCTAAAACTCT+CGG	0.489363	2:-18604892	None:intergenic
TCATAGCATAGAAATCAAAT+CGG	0.504077	2:-18605023	None:intergenic
GAGGCTCGATTAGAAAGAAT+CGG	0.553245	2:+18604958	MsG0280007654.01.T01:CDS
GTAATTCTTTCTACTTCTAG+GGG	0.588562	2:+18604919	MsG0280007654.01.T01:CDS
TTAGAATAGATTCGTAGACC+AGG	0.649538	2:-18604856	None:intergenic
GGGTATAATGACAGATCGAG+AGG	0.665637	2:+18604939	MsG0280007654.01.T01:CDS
GCTCGATTAGAAAGAATCGG+CGG	0.737146	2:+18604961	MsG0280007654.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	GAAGTTTTATGTTATATATA+TGG	+	Chr2:18604985-18605004	MsG0280007654.01.T01:CDS	15.0%
!!!	AAGTATTTTCACAATGTTTT+CGG	-	Chr2:18604701-18604720	None:intergenic	20.0%
!!!	ACTTTTTAGTTTTAACTCTA+CGG	+	Chr2:18604773-18604792	MsG0280007654.01.T01:CDS	20.0%
!	ATAGAAATCAAATCGGATAT+CGG	-	Chr2:18605019-18605038	None:intergenic	25.0%
!	TCATAGCATAGAAATCAAAT+CGG	-	Chr2:18605026-18605045	None:intergenic	25.0%
!	TGACATGAATAGAAAAAGAA+CGG	+	Chr2:18604651-18604670	MsG0280007654.01.T01:CDS	25.0%
!	TGTAATTCTTTCTACTTCTA+GGG	+	Chr2:18604918-18604937	MsG0280007654.01.T01:CDS	25.0%
!	TTGTAATTCTTTCTACTTCT+AGG	+	Chr2:18604917-18604936	MsG0280007654.01.T01:CDS	25.0%
	GTAATTCTTTCTACTTCTAG+GGG	+	Chr2:18604919-18604938	MsG0280007654.01.T01:CDS	30.0%
!	GTGAAAATACTTTTACGAGA+GGG	+	Chr2:18604709-18604728	MsG0280007654.01.T01:CDS	30.0%
!	TGTGAAAATACTTTTACGAG+AGG	+	Chr2:18604708-18604727	MsG0280007654.01.T01:CDS	30.0%
!	TTTTCGGTGATATTAGTAAG+TGG	-	Chr2:18604685-18604704	None:intergenic	30.0%
	CTCTACGGTATAGAAGAAAT+AGG	+	Chr2:18604788-18604807	MsG0280007654.01.T01:CDS	35.0%
	TTAAAAAGGATCAGTACACC+TGG	+	Chr2:18604838-18604857	MsG0280007654.01.T01:CDS	35.0%
	TTAGAATAGATTCGTAGACC+AGG	-	Chr2:18604859-18604878	None:intergenic	35.0%
!	CAACAAATTCCGAGAGTTTT+AGG	+	Chr2:18604883-18604902	MsG0280007654.01.T01:CDS	35.0%
!!!	AAATTCCGAGAGTTTTAGGA+GGG	+	Chr2:18604887-18604906	MsG0280007654.01.T01:CDS	35.0%
	GAGGCTCGATTAGAAAGAAT+CGG	+	Chr2:18604958-18604977	MsG0280007654.01.T01:CDS	40.0%
!!!	CAAATTCCGAGAGTTTTAGG+AGG	+	Chr2:18604886-18604905	MsG0280007654.01.T01:CDS	40.0%
	GCTCGATTAGAAAGAATCGG+CGG	+	Chr2:18604961-18604980	MsG0280007654.01.T01:CDS	45.0%
	GGGTATAATGACAGATCGAG+AGG	+	Chr2:18604939-18604958	MsG0280007654.01.T01:CDS	45.0%
!!!	AAACTTTTTTAAATTTAAAA+AGG	+	Chr2:18604824-18604843	MsG0280007654.01.T01:CDS	5.0%
	CCCATCCCTCCTAAAACTCT+CGG	-	Chr2:18604895-18604914	None:intergenic	50.0%
!!!	CCGAGAGTTTTAGGAGGGAT+GGG	+	Chr2:18604892-18604911	MsG0280007654.01.T01:CDS	50.0%
!!!	CGAGAGTTTTAGGAGGGATG+GGG	+	Chr2:18604893-18604912	MsG0280007654.01.T01:CDS	50.0%
!!!	TCCGAGAGTTTTAGGAGGGA+TGG	+	Chr2:18604891-18604910	MsG0280007654.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	18604628	18605068	18604628	ID=MsG0280007654.01;Name=MsG0280007654.01
Chr2	mRNA	18604628	18605068	18604628	ID=MsG0280007654.01.T01;Parent=MsG0280007654.01;Name=MsG0280007654.01.T01;_AED=0.46;_eAED=0.46;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|146
Chr2	exon	18604628	18605068	18604628	ID=MsG0280007654.01.T01:exon:4273;Parent=MsG0280007654.01.T01
Chr2	CDS	18604628	18605068	18604628	ID=MsG0280007654.01.T01:cds;Parent=MsG0280007654.01.T01
Chr2	mRNA	18604655	18605068	18604655	ID=MsG0280007654.01.T02;Parent=MsG0280007654.01;Name=MsG0280007654.01.T02;_AED=0.46;_eAED=0.46;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|137
Chr2	exon	18604655	18605068	18604655	ID=MsG0280007654.01.T02:exon:4274;Parent=MsG0280007654.01.T02
Chr2	CDS	18604655	18605068	18604655	ID=MsG0280007654.01.T02:cds;Parent=MsG0280007654.01.T02
Chr2	mRNA	18604910	18605068	18604910	ID=MsG0280007654.01.T03;Parent=MsG0280007654.01;Name=MsG0280007654.01.T03;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|52
Chr2	exon	18604910	18605068	18604910	ID=MsG0280007654.01.T03:exon:4275;Parent=MsG0280007654.01.T03
Chr2	CDS	18604910	18605068	18604910	ID=MsG0280007654.01.T03:cds;Parent=MsG0280007654.01.T03
Chr2	mRNA	18604946	18605068	18604946	ID=MsG0280007654.01.T04;Parent=MsG0280007654.01;Name=MsG0280007654.01.T04;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|40
Chr2	exon	18604946	18605068	18604946	ID=MsG0280007654.01.T04:exon:4276;Parent=MsG0280007654.01.T04
Chr2	CDS	18604946	18605068	18604946	ID=MsG0280007654.01.T04:cds;Parent=MsG0280007654.01.T04

Gene Sequence

>MsG0280007654.01.T01

ATGCTAACTTTGATACGCAATGCTGACATGAATAGAAAAAGAACGGTTCAAATACCACTTACTAATATCACCGAAAACATTGTGAAAATACTTTTACGAGAGGGTTTTGTTGAAAACGTTAGAAAACATCGAGAAAGCAACAAATACTTTTTAGTTTTAACTCTACGGTATAGAAGAAATAGGAAAGAATCGTATAAAACTTTTTTAAATTTAAAAAGGATCAGTACACCTGGTCTACGAATCTATTCTAACTATCAACAAATTCCGAGAGTTTTAGGAGGGATGGGGATTGTAATTCTTTCTACTTCTAGGGGTATAATGACAGATCGAGAGGCTCGATTAGAAAGAATCGGCGGCGAAGTTTTATGTTATATATATGGTAAATTTGCCGATATCCGATTTGATTTCTATGCTATGAAAAAATTATATACATTATTGTAA

>MsG0280007654.01.T04

ATGACAGATCGAGAGGCTCGATTAGAAAGAATCGGCGGCGAAGTTTTATGTTATATATATGGTAAATTTGCCGATATCCGATTTGATTTCTATGCTATGAAAAAATTATATACATTATTGTAA

>MsG0280007654.01.T02

ATGAATAGAAAAAGAACGGTTCAAATACCACTTACTAATATCACCGAAAACATTGTGAAAATACTTTTACGAGAGGGTTTTGTTGAAAACGTTAGAAAACATCGAGAAAGCAACAAATACTTTTTAGTTTTAACTCTACGGTATAGAAGAAATAGGAAAGAATCGTATAAAACTTTTTTAAATTTAAAAAGGATCAGTACACCTGGTCTACGAATCTATTCTAACTATCAACAAATTCCGAGAGTTTTAGGAGGGATGGGGATTGTAATTCTTTCTACTTCTAGGGGTATAATGACAGATCGAGAGGCTCGATTAGAAAGAATCGGCGGCGAAGTTTTATGTTATATATATGGTAAATTTGCCGATATCCGATTTGATTTCTATGCTATGAAAAAATTATATACATTATTGTAA

>MsG0280007654.01.T03

ATGGGGATTGTAATTCTTTCTACTTCTAGGGGTATAATGACAGATCGAGAGGCTCGATTAGAAAGAATCGGCGGCGAAGTTTTATGTTATATATATGGTAAATTTGCCGATATCCGATTTGATTTCTATGCTATGAAAAAATTATATACATTATTGTAA

Protein sequence

>MsG0280007654.01.T01

MLTLIRNADMNRKRTVQIPLTNITENIVKILLREGFVENVRKHRESNKYFLVLTLRYRRNRKESYKTFLNLKRISTPGLRIYSNYQQIPRVLGGMGIVILSTSRGIMTDREARLERIGGEVLCYIYGKFADIRFDFYAMKKLYTLL*

>MsG0280007654.01.T04

MTDREARLERIGGEVLCYIYGKFADIRFDFYAMKKLYTLL*

>MsG0280007654.01.T02

MNRKRTVQIPLTNITENIVKILLREGFVENVRKHRESNKYFLVLTLRYRRNRKESYKTFLNLKRISTPGLRIYSNYQQIPRVLGGMGIVILSTSRGIMTDREARLERIGGEVLCYIYGKFADIRFDFYAMKKLYTLL*

>MsG0280007654.01.T03

MGIVILSTSRGIMTDREARLERIGGEVLCYIYGKFADIRFDFYAMKKLYTLL*