AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380014037.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014037.01.T01 MTR_4g051110 97.917 96 2 0 1 96 140 235 2.05e-65 197
MsG0380014037.01.T01 MTR_7g116690 90.625 96 9 0 1 96 114 209 2.89e-59 180
MsG0380014037.01.T01 MTR_0002s0530 76.744 86 20 0 1 86 140 225 9.92e-43 145
MsG0380014037.01.T01 MTR_3g029540 95.349 43 2 0 32 74 44 86 1.18e-23 86.3
MsG0380014037.01.T01 MTR_7g012800 97.368 38 1 0 59 96 139 176 3.07e-18 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014037.01.T01 ATCG00160 91.667 96 8 0 1 96 140 235 5.79e-60 183

Find 17 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTCATTTGGAAACATATC+TGG 0.181531 3:-48714918 MsG0380014037.01.T01:CDS
CAAGAGACAATTATCTCATT+TGG 0.294663 3:-48714930 MsG0380014037.01.T01:CDS
ATCAACGATTATTACAATAT+CGG 0.341941 3:+48714868 None:intergenic
CTAATTTGTTAAGAATTAAT+CGG 0.359442 3:+48714711 None:intergenic
ATCGAAATATCTGCTAGATC+TGG 0.363586 3:+48714764 None:intergenic
CTTCGAGAATGTATCACTTT+GGG 0.388824 3:-48714824 MsG0380014037.01.T01:CDS
TGATCAGCAAGAAGAATATA+CGG 0.437056 3:-48714849 MsG0380014037.01.T01:CDS
TCTTCGAGAATGTATCACTT+TGG 0.477646 3:-48714825 MsG0380014037.01.T01:CDS
TCAACGATTATTACAATATC+GGG 0.480431 3:+48714869 None:intergenic
GTATCGATTAAACAAATCGT+TGG 0.491426 3:+48714797 None:intergenic
GCTATAGCATCATCATTAGC+TGG 0.518853 3:+48714740 None:intergenic
TCGAAATATCTGCTAGATCT+GGG 0.525094 3:+48714765 None:intergenic
TCTCATTTGGAAACATATCT+GGG 0.550552 3:-48714917 MsG0380014037.01.T01:CDS
GGGTGGCATAAAATATATGA+CGG 0.585418 3:-48714897 MsG0380014037.01.T01:CDS
GGTGGCATAAAATATATGAC+GGG 0.610359 3:-48714896 MsG0380014037.01.T01:CDS
CATTTGGAAACATATCTGGG+TGG 0.732935 3:-48714914 MsG0380014037.01.T01:CDS
GTGGCATAAAATATATGACG+GGG 0.737363 3:-48714895 MsG0380014037.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTAATTTGTTAAGAATTAAT+CGG + Chr3:48714898-48714917 None:intergenic 15.0%
!! ATCAACGATTATTACAATAT+CGG + Chr3:48714741-48714760 None:intergenic 20.0%
! TCAACGATTATTACAATATC+GGG + Chr3:48714740-48714759 None:intergenic 25.0%
!!! ATTAGTTTTTGCAATTTGTG+AGG - Chr3:48714913-48714932 MsG0380014037.01.T01:CDS 25.0%
!!! TTAGTTTTTGCAATTTGTGA+GGG - Chr3:48714914-48714933 MsG0380014037.01.T01:CDS 25.0%
ATCTCATTTGGAAACATATC+TGG - Chr3:48714688-48714707 MsG0380014037.01.T01:CDS 30.0%
CAAGAGACAATTATCTCATT+TGG - Chr3:48714676-48714695 MsG0380014037.01.T01:CDS 30.0%
TCTCATTTGGAAACATATCT+GGG - Chr3:48714689-48714708 MsG0380014037.01.T01:CDS 30.0%
TGATCAGCAAGAAGAATATA+CGG - Chr3:48714757-48714776 MsG0380014037.01.T01:CDS 30.0%
!! GTATCGATTAAACAAATCGT+TGG + Chr3:48714812-48714831 None:intergenic 30.0%
ATCGAAATATCTGCTAGATC+TGG + Chr3:48714845-48714864 None:intergenic 35.0%
CTTCGAGAATGTATCACTTT+GGG - Chr3:48714782-48714801 MsG0380014037.01.T01:CDS 35.0%
GGGTGGCATAAAATATATGA+CGG - Chr3:48714709-48714728 MsG0380014037.01.T01:CDS 35.0%
GGTGGCATAAAATATATGAC+GGG - Chr3:48714710-48714729 MsG0380014037.01.T01:CDS 35.0%
GTGGCATAAAATATATGACG+GGG - Chr3:48714711-48714730 MsG0380014037.01.T01:CDS 35.0%
TCGAAATATCTGCTAGATCT+GGG + Chr3:48714844-48714863 None:intergenic 35.0%
TCTTCGAGAATGTATCACTT+TGG - Chr3:48714781-48714800 MsG0380014037.01.T01:CDS 35.0%
CATTTGGAAACATATCTGGG+TGG - Chr3:48714692-48714711 MsG0380014037.01.T01:CDS 40.0%
GCTATAGCATCATCATTAGC+TGG + Chr3:48714869-48714888 None:intergenic 40.0%
Chromosome Type Strat End Strand Name
Chr3 gene 48714670 48714958 48714670 ID=MsG0380014037.01;Name=MsG0380014037.01
Chr3 mRNA 48714670 48714958 48714670 ID=MsG0380014037.01.T01;Parent=MsG0380014037.01;Name=MsG0380014037.01.T01;_AED=0.03;_eAED=0.03;_QI=1|-1|0|1|-1|0|1|0|96
Chr3 exon 48714670 48714958 48714670 ID=MsG0380014037.01.T01:exon:20705;Parent=MsG0380014037.01.T01
Chr3 five_prime_UTR 48714958 48714958 48714958 ID=MsG0380014037.01.T01:five_prime_utr;Parent=MsG0380014037.01.T01
Chr3 CDS 48714670 48714957 48714670 ID=MsG0380014037.01.T01:cds;Parent=MsG0380014037.01.T01
Gene Sequence

>MsG0380014037.01.T01

ATGTTCAAGAGACAATTATCTCATTTGGAAACATATCTGGGTGGCATAAAATATATGACGGGGTTACCCGATATTGTAATAATCGTTGATCAGCAAGAAGAATATACGGCTCTTCGAGAATGTATCACTTTGGGAATTCCAACGATTTGTTTAATCGATACAAATTGTGACCCAGATCTAGCAGATATTTCGATTCCAGCTAATGATGATGCTATAGCTTCAATCCGATTAATTCTTAACAAATTAGTTTTTGCAATTTGTGAGGGTCGTTCTAGCTATATACGAAAT

Protein sequence

>MsG0380014037.01.T01

MFKRQLSHLETYLGGIKYMTGLPDIVIIVDQQEEYTALRECITLGIPTICLIDTNCDPDLADISIPANDDAIASIRLILNKLVFAICEGRSSYIRN