AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007953.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007953.01.T01 MTR_2g435930 97.830 507 11 0 1 507 1 507 0.0 1012
MsG0280007953.01.T01 MTR_6g043810 47.752 467 235 2 11 476 25 483 1.08e-159 464
MsG0280007953.01.T01 MTR_6g043810 47.752 467 235 2 11 476 15 473 1.26e-159 463
MsG0280007953.01.T01 MTR_3g111020 46.458 480 241 2 2 481 34 497 2.73e-151 444
MsG0280007953.01.T01 MTR_5g067460 45.860 471 239 2 11 481 47 501 1.02e-147 435
MsG0280007953.01.T01 MTR_0039s0080 43.164 512 260 4 1 507 17 502 1.09e-145 428
MsG0280007953.01.T01 MTR_1g101500 45.816 478 227 3 8 478 55 507 4.24e-141 417
MsG0280007953.01.T01 MTR_5g010830 43.856 472 247 3 5 476 53 506 4.36e-135 402
MsG0280007953.01.T01 MTR_8g102430 42.918 473 231 6 7 476 8 444 3.92e-132 391
MsG0280007953.01.T01 MTR_6g088805 42.308 468 251 5 17 484 25 473 7.91e-131 389
MsG0280007953.01.T01 MTR_4g027920 39.746 473 263 2 13 481 150 604 4.08e-120 366
MsG0280007953.01.T01 MTR_5g011540 32.298 483 300 3 4 480 28 489 1.69e-79 257
MsG0280007953.01.T01 MTR_6g081410 28.184 479 330 2 1 479 7 471 4.24e-73 239
MsG0280007953.01.T01 MTR_6g081810 29.175 473 293 3 5 477 13 443 1.36e-72 238
MsG0280007953.01.T01 MTR_6g081240 31.223 458 291 6 16 469 7 444 1.00e-70 233
MsG0280007953.01.T01 MTR_6g081300 30.228 483 312 5 16 494 7 468 1.25e-68 228
MsG0280007953.01.T01 MTR_6g081400 32.043 465 295 5 16 477 12 458 3.38e-68 226
MsG0280007953.01.T01 MTR_6g081780 26.804 485 329 3 1 479 7 471 5.08e-68 226
MsG0280007953.01.T01 MTR_6g066380 28.184 479 328 2 1 477 7 471 1.59e-67 225
MsG0280007953.01.T01 MTR_6g004220 29.512 471 315 3 8 477 12 466 2.87e-65 219
MsG0280007953.01.T01 MTR_8g106670 27.423 485 333 3 17 498 10 478 2.94e-65 219
MsG0280007953.01.T01 MTR_1g108810 28.728 456 305 4 16 469 31 468 7.24e-65 218
MsG0280007953.01.T01 MTR_1g108840 28.602 472 320 3 4 474 25 480 9.36e-65 218
MsG0280007953.01.T01 MTR_6g004220 29.590 463 309 3 8 469 19 465 1.89e-63 215
MsG0280007953.01.T01 MTR_6g081310 30.213 470 286 6 16 473 7 446 3.03e-63 213
MsG0280007953.01.T01 MTR_6g081390 29.374 463 310 3 16 477 9 455 8.39e-61 207
MsG0280007953.01.T01 MTR_7g082810 29.735 491 311 9 13 495 14 478 2.07e-60 206
MsG0280007953.01.T01 MTR_1g109060 28.633 461 309 4 19 477 24 466 6.92e-57 197
MsG0280007953.01.T01 MTR_7g082800 31.111 405 259 2 78 480 1 387 7.02e-56 192
MsG0280007953.01.T01 MTR_1g109060 28.698 453 303 4 19 469 24 458 1.55e-54 191
MsG0280007953.01.T01 MTR_1g108990 27.586 464 318 2 19 481 25 471 2.05e-50 179
MsG0280007953.01.T01 MTR_6g004220 29.058 382 254 3 8 388 19 384 5.83e-48 172
MsG0280007953.01.T01 MTR_2g078930 25.758 462 319 2 18 475 42 483 6.10e-48 173
MsG0280007953.01.T01 MTR_8g069470 26.598 485 319 10 6 477 31 491 3.97e-45 166
MsG0280007953.01.T01 MTR_2g079020 25.161 465 326 3 18 480 36 480 1.91e-44 164
MsG0280007953.01.T01 MTR_2g078960 25.806 465 323 2 18 479 32 477 1.21e-43 161
MsG0280007953.01.T01 MTR_5g033100 24.249 466 332 5 16 479 53 499 4.92e-41 154
MsG0280007953.01.T01 MTR_4g048000 24.236 458 325 3 20 474 65 503 4.96e-41 154
MsG0280007953.01.T01 MTR_2g022980 24.396 455 324 4 25 475 50 488 8.43e-41 153
MsG0280007953.01.T01 MTR_2g088400 24.508 457 323 3 20 473 53 490 1.18e-40 153
MsG0280007953.01.T01 MTR_2g078970 24.729 461 325 2 18 475 30 471 3.38e-40 152
MsG0280007953.01.T01 MTR_6g047610 26.350 463 298 8 19 469 31 462 2.66e-39 149
MsG0280007953.01.T01 MTR_5g032720 25.806 465 321 7 14 474 31 475 4.94e-38 145
MsG0280007953.01.T01 MTR_1g021740 23.529 476 347 3 2 477 18 476 1.25e-37 144
MsG0280007953.01.T01 MTR_5g032750 25.541 462 322 7 16 474 33 475 3.48e-37 143
MsG0280007953.01.T01 MTR_6g004220 29.073 313 205 3 8 319 19 315 1.62e-36 138
MsG0280007953.01.T01 MTR_2g088400 24.533 428 300 4 20 444 53 460 8.26e-36 139
MsG0280007953.01.T01 MTR_8g063590 26.097 433 303 5 50 481 84 500 2.13e-35 139
MsG0280007953.01.T01 MTR_5g090290 25.478 471 324 7 31 493 54 505 1.74e-33 134
MsG0280007953.01.T01 MTR_1g100180 24.612 451 320 4 20 469 44 475 1.86e-33 133
MsG0280007953.01.T01 MTR_7g087410 23.878 490 336 6 16 497 28 488 4.05e-33 132
MsG0280007953.01.T01 MTR_3g099720 22.037 481 358 3 4 484 26 489 1.20e-32 130
MsG0280007953.01.T01 MTR_7g087430 24.570 407 276 3 78 477 1 383 8.04e-32 127
MsG0280007953.01.T01 MTR_3g099700 24.109 477 340 6 4 477 31 488 2.39e-31 127
MsG0280007953.01.T01 MTR_7g087400 22.197 437 306 3 49 477 60 470 4.25e-31 126
MsG0280007953.01.T01 MTR_6g047640 23.736 455 302 6 19 469 31 444 4.50e-30 122
MsG0280007953.01.T01 MTR_7g087410 23.239 426 294 4 78 497 1 399 1.21e-29 120
MsG0280007953.01.T01 MTR_6g047640 24.396 455 318 5 19 469 31 463 1.55e-29 121
MsG0280007953.01.T01 MTR_6g047590 24.390 451 322 3 19 469 31 462 6.39e-29 119
MsG0280007953.01.T01 MTR_7g087370 21.294 479 314 4 7 477 18 441 1.13e-25 110
MsG0280007953.01.T01 MTR_6g027200 22.266 503 354 6 3 490 20 500 1.11e-23 104
MsG0280007953.01.T01 MTR_5g087930 22.319 457 310 7 34 483 41 459 1.86e-21 97.4
MsG0280007953.01.T01 MTR_6g027190 22.505 471 330 8 8 469 13 457 1.69e-19 91.7
MsG0280007953.01.T01 MTR_2g078990 24.766 214 161 0 262 475 87 300 5.23e-17 82.4
MsG0280007953.01.T01 MTR_2g079030 26.923 208 148 3 274 480 319 523 3.40e-15 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007953.01.T01 AT5G49130 62.279 509 175 4 1 500 1 501 0.0 619
MsG0280007953.01.T01 AT1G71870 46.154 494 255 4 3 486 7 499 2.05e-154 450
MsG0280007953.01.T01 AT4G29140 43.114 501 262 4 4 499 40 522 9.45e-144 424
MsG0280007953.01.T01 AT5G19700 43.408 493 255 6 4 492 20 492 1.30e-137 407
MsG0280007953.01.T01 AT5G52050 41.770 486 266 3 18 501 33 503 1.00e-135 402
MsG0280007953.01.T01 AT1G58340 44.161 471 247 3 9 479 47 501 7.16e-133 396
MsG0280007953.01.T01 AT4G23030 43.265 490 243 5 5 483 14 479 1.77e-120 363
MsG0280007953.01.T01 AT2G38510 37.759 482 282 4 16 497 2 465 1.75e-118 357
MsG0280007953.01.T01 AT4G22790 33.400 503 300 6 1 492 1 479 2.24e-78 254
MsG0280007953.01.T01 AT5G52450 29.918 488 322 4 10 494 13 483 2.55e-67 224
MsG0280007953.01.T01 AT1G15150 29.913 458 301 3 20 475 31 470 2.11e-64 217
MsG0280007953.01.T01 AT1G15170 30.263 456 302 2 20 475 34 473 4.16e-64 216
MsG0280007953.01.T01 AT1G64820 30.567 494 314 6 18 505 27 497 4.73e-64 216
MsG0280007953.01.T01 AT1G71140 29.374 463 307 4 20 480 26 470 9.72e-61 207
MsG0280007953.01.T01 AT2G04080 28.992 476 318 4 4 477 11 468 1.15e-60 207
MsG0280007953.01.T01 AT2G34360 28.169 497 322 6 1 487 1 472 1.56e-60 206
MsG0280007953.01.T01 AT1G66760 29.221 462 311 3 19 480 27 472 2.60e-60 206
MsG0280007953.01.T01 AT1G15160 29.847 459 298 5 20 474 31 469 2.88e-60 206
MsG0280007953.01.T01 AT2G04100 28.390 472 321 3 7 477 16 471 6.88e-60 205
MsG0280007953.01.T01 AT1G15180 31.659 458 293 4 20 475 35 474 1.19e-59 204
MsG0280007953.01.T01 AT2G04070 29.451 455 305 2 20 474 27 465 2.14e-59 203
MsG0280007953.01.T01 AT2G04080 29.060 468 312 4 4 469 11 460 3.87e-59 202
MsG0280007953.01.T01 AT2G04050 31.317 463 298 4 14 474 21 465 4.58e-59 202
MsG0280007953.01.T01 AT2G04040 29.299 471 316 3 7 477 15 468 5.19e-59 202
MsG0280007953.01.T01 AT2G04100 28.448 464 315 3 7 469 16 463 3.46e-58 200
MsG0280007953.01.T01 AT1G66760 29.268 451 303 3 19 469 27 461 1.34e-56 196
MsG0280007953.01.T01 AT1G66780 29.114 474 317 3 4 477 22 476 1.58e-56 196
MsG0280007953.01.T01 AT1G73700 28.419 468 319 2 13 480 17 468 4.02e-56 195
MsG0280007953.01.T01 AT2G04090 26.819 481 331 5 6 483 15 477 1.82e-54 190
MsG0280007953.01.T01 AT1G15170 29.973 367 241 2 109 475 4 354 4.24e-52 181
MsG0280007953.01.T01 AT1G11670 25.714 455 322 4 20 474 50 488 2.42e-49 177
MsG0280007953.01.T01 AT1G61890 26.593 455 314 5 25 475 48 486 9.59e-48 172
MsG0280007953.01.T01 AT1G15150 29.396 364 237 3 114 475 9 354 1.35e-46 166
MsG0280007953.01.T01 AT1G61890 25.482 467 328 5 7 469 30 480 3.14e-46 168
MsG0280007953.01.T01 AT3G23550 26.872 454 310 3 18 468 29 463 1.99e-43 160
MsG0280007953.01.T01 AT1G66760 29.348 368 244 3 19 386 27 378 3.66e-43 158
MsG0280007953.01.T01 AT3G21690 24.835 455 326 3 20 474 52 490 6.71e-42 157
MsG0280007953.01.T01 AT3G26590 24.729 461 320 7 20 475 45 483 2.31e-41 155
MsG0280007953.01.T01 AT1G61890 25.688 436 304 5 7 438 30 449 2.95e-41 154
MsG0280007953.01.T01 AT4G21903 26.047 430 291 6 50 474 83 490 3.45e-41 155
MsG0280007953.01.T01 AT3G59030 26.593 455 308 6 34 483 65 498 4.91e-41 154
MsG0280007953.01.T01 AT4G21910 25.495 455 322 5 20 474 55 492 1.12e-40 153
MsG0280007953.01.T01 AT4G21910 25.495 455 322 5 20 474 55 492 1.12e-40 153
MsG0280007953.01.T01 AT4G21903 25.676 444 299 7 50 484 83 504 3.47e-40 152
MsG0280007953.01.T01 AT5G38030 24.473 474 328 8 20 485 45 496 6.19e-40 151
MsG0280007953.01.T01 AT4G21910 24.781 456 326 5 20 475 57 495 5.22e-39 149
MsG0280007953.01.T01 AT4G21910 24.781 456 326 5 20 475 57 495 5.22e-39 149
MsG0280007953.01.T01 AT3G23560 25.212 472 327 4 4 468 19 471 1.21e-38 147
MsG0280007953.01.T01 AT4G21903 25.377 398 273 4 81 474 4 381 1.37e-38 145
MsG0280007953.01.T01 AT3G26590 24.623 398 277 5 81 475 4 381 3.56e-38 144
MsG0280007953.01.T01 AT1G12950 24.464 466 319 8 20 477 67 507 6.24e-38 146
MsG0280007953.01.T01 AT4G00350 24.017 458 330 2 18 475 86 525 1.74e-37 145
MsG0280007953.01.T01 AT5G44050 25.208 480 326 8 2 474 25 478 5.39e-37 143
MsG0280007953.01.T01 AT1G66760 27.666 347 235 3 123 469 3 333 3.48e-35 134
MsG0280007953.01.T01 AT1G15180 30.496 282 176 4 109 388 4 267 3.89e-34 133
MsG0280007953.01.T01 AT1G47530 23.176 466 333 5 16 477 31 475 7.68e-34 134
MsG0280007953.01.T01 AT4G25640 23.060 451 327 2 20 469 34 465 6.14e-33 131
MsG0280007953.01.T01 AT1G23300 24.587 484 337 5 3 477 20 484 8.13e-33 131
MsG0280007953.01.T01 AT4G25640 23.060 451 327 2 20 469 34 465 8.61e-33 131
MsG0280007953.01.T01 AT3G23560 25.459 381 258 4 4 377 19 380 9.91e-32 127
MsG0280007953.01.T01 AT1G33090 25.000 476 322 7 8 469 14 468 3.04e-31 126
MsG0280007953.01.T01 AT5G10420 24.190 463 327 6 16 474 34 476 3.81e-31 126
MsG0280007953.01.T01 AT5G65380 24.896 482 339 5 2 477 14 478 2.68e-30 124
MsG0280007953.01.T01 AT1G33100 24.558 452 320 4 20 469 33 465 3.98e-30 123
MsG0280007953.01.T01 AT1G33110 24.051 474 329 5 8 469 14 468 4.18e-29 120
MsG0280007953.01.T01 AT1G47530 23.250 400 282 5 82 477 5 383 2.97e-28 116
MsG0280007953.01.T01 AT4G25640 22.512 422 307 2 20 440 34 436 4.32e-28 117
MsG0280007953.01.T01 AT1G33080 23.557 433 300 5 49 474 65 473 1.25e-27 116
MsG0280007953.01.T01 AT2G34360 26.522 230 162 1 1 223 1 230 5.17e-27 113
MsG0280007953.01.T01 AT2G34360 34.091 176 114 1 312 487 257 430 1.47e-20 94.7
MsG0280007953.01.T01 AT1G33080 23.877 423 301 4 49 469 65 468 5.43e-27 114
MsG0280007953.01.T01 AT3G59030 25.815 368 247 6 34 396 65 411 4.44e-26 110
MsG0280007953.01.T01 AT3G03620 22.316 475 336 6 3 469 20 469 1.22e-24 107
MsG0280007953.01.T01 AT3G03620 22.316 475 336 6 3 469 20 469 1.22e-24 107
MsG0280007953.01.T01 AT5G17700 20.668 479 347 6 5 475 19 472 2.11e-21 97.8
MsG0280007953.01.T01 AT5G17700 20.668 479 347 6 5 475 19 472 2.11e-21 97.8

Find 125 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCGGTTACTCAGTCCTTTC+AGG 0.200835 2:+23643705 MsG0280007953.01.T01:CDS
GTTCAACATTAGGGTTATTA+TGG 0.202311 2:+23644546 MsG0280007953.01.T01:CDS
CAGTTGTCGGGTATGTCTTT+TGG 0.247868 2:-23643100 None:intergenic
GGAGTGCAAGGCCAGGTATT+GGG 0.276246 2:+23644711 MsG0280007953.01.T01:CDS
GGGTTATTATGGACAACATT+AGG 0.276633 2:+23644557 MsG0280007953.01.T01:CDS
AAGAATGGGGCATGTTAATA+AGG 0.284500 2:+23644264 MsG0280007953.01.T01:CDS
CTTGCTCACATTTGACTGTT+TGG 0.296645 2:-23644970 None:intergenic
GAAGAAAAGGGTATTGAAAT+TGG 0.308573 2:-23644123 None:intergenic
ACTATGCTCATGGCTGCTAT+TGG 0.316954 2:-23643585 None:intergenic
TCACTAGCAAGTTCAACATT+AGG 0.321641 2:+23644536 MsG0280007953.01.T01:CDS
TGTAGAACATGTATGATAAA+AGG 0.326149 2:-23644145 None:intergenic
GTTGACAAATTTGCCTTTGT+TGG 0.351859 2:-23644494 None:intergenic
CACTAGCAAGTTCAACATTA+GGG 0.353538 2:+23644537 MsG0280007953.01.T01:CDS
AACTCACAAACACCAATTAT+AGG 0.359394 2:-23644647 None:intergenic
TGAGTTAGCAAGTGGTGCTT+TGG 0.364654 2:+23643662 MsG0280007953.01.T01:CDS
CTACGTAAATAGATGCGAAT+TGG 0.368924 2:-23643972 None:intergenic
GGTTATTATGGACAACATTA+GGG 0.377545 2:+23644558 MsG0280007953.01.T01:CDS
ACTAGTTGTGGAATGCTTAG+AGG 0.390010 2:+23644689 MsG0280007953.01.T01:CDS
AAAGGCAAATTTGTCAACTA+TGG 0.393581 2:+23644499 MsG0280007953.01.T01:CDS
TGTATGATAAAAGGAAGAAA+AGG 0.401130 2:-23644136 None:intergenic
GCAAGTGGTGCTTTGGCCAT+AGG 0.404906 2:+23643669 MsG0280007953.01.T01:CDS
ATTAAGCCACAAGAGTGAAA+TGG 0.405706 2:-23643841 None:intergenic
CTATGCTCATGGCTGCTATT+GGG 0.407811 2:-23643584 None:intergenic
TTGTCAACTATGGTAGCAAT+TGG 0.413667 2:+23644509 MsG0280007953.01.T01:CDS
GGGAGTGCAAGGCCAGGTAT+TGG 0.419436 2:+23644710 MsG0280007953.01.T01:CDS
AAGGTTAAGAAAATGATGAT+AGG 0.422391 2:-23644053 None:intergenic
ACCTGAAAGGACTGAGTAAC+CGG 0.424046 2:-23643706 None:intergenic
AACCCCAACACTATTCCTAT+TGG 0.430761 2:-23644773 None:intergenic
GTCTTGGAGTTTGCTTAGAA+TGG 0.433878 2:+23644303 MsG0280007953.01.T01:CDS
GATGATAACAAGGGAGGACT+AGG 0.437114 2:-23644203 None:intergenic
AGTGAAGTACACCACAATAA+TGG 0.439407 2:-23644011 None:intergenic
AGTTGTTTGCATGGGAAAGT+TGG 0.441804 2:+23643632 MsG0280007953.01.T01:CDS
GCGCCAATAGGAATAGTGTT+GGG 0.442712 2:+23644770 MsG0280007953.01.T01:CDS
CATGATCTTAGTTGTTTGCA+TGG 0.446886 2:+23643623 MsG0280007953.01.T01:CDS
TGATGAAGGGATAGCATGAA+TGG 0.452307 2:-23643870 None:intergenic
TTAAGCCACAAGAGTGAAAT+GGG 0.455813 2:-23643840 None:intergenic
GTTGTTTGCATGGGAAAGTT+GGG 0.456332 2:+23643633 MsG0280007953.01.T01:CDS
TACACCAAGGTGAAGTTTGA+AGG 0.461500 2:-23644072 None:intergenic
CATGCCCCATTCTTTGCCTA+GGG 0.463542 2:-23644255 None:intergenic
TTTATCCCTGCCCCAATACC+TGG 0.465679 2:-23644722 None:intergenic
ACATGCCCCATTCTTTGCCT+AGG 0.465944 2:-23644256 None:intergenic
CATTACCCATTTCACTCTTG+TGG 0.466604 2:+23643835 MsG0280007953.01.T01:CDS
TTGTGGTATATAACACTGAT+TGG 0.471072 2:+23644873 MsG0280007953.01.T01:CDS
TCAAAACCCTAGGCAAAGAA+TGG 0.471654 2:+23644249 MsG0280007953.01.T01:CDS
ATAGCAGCCATGAGCATAGT+AGG 0.475953 2:+23643588 MsG0280007953.01.T01:CDS
AACTCCAAGACAACTTTGAA+TGG 0.476284 2:-23644291 None:intergenic
CAAAACCCTAGGCAAAGAAT+GGG 0.476376 2:+23644250 MsG0280007953.01.T01:CDS
GCAAGGCCAGGTATTGGGGC+AGG 0.476495 2:+23644716 MsG0280007953.01.T01:CDS
ATAGGCTTCACCAATGTAAC+CGG 0.479266 2:+23643687 MsG0280007953.01.T01:CDS
ATTCCACAACTAGTTGTTTG+TGG 0.480040 2:-23644680 None:intergenic
AAACTTCACCTTGGTGTACA+AGG 0.480431 2:+23644077 MsG0280007953.01.T01:CDS
ATCGTACCTCAGCAGTTGTC+GGG 0.482070 2:-23643112 None:intergenic
GAATCGAGAGGCGGCAATAG+AGG 0.489527 2:-23643920 None:intergenic
GAGGTATCCTACTATGCTCA+TGG 0.490636 2:-23643595 None:intergenic
GATAGGGATATGAATGATAA+CGG 0.502534 2:-23644036 None:intergenic
AAGCTATTCGCTATAAGATC+GGG 0.504614 2:-23643942 None:intergenic
GAGTGCAAGGCCAGGTATTG+GGG 0.506676 2:+23644712 MsG0280007953.01.T01:CDS
GTATGATAAAAGGAAGAAAA+GGG 0.508071 2:-23644135 None:intergenic
GGTTACATTGGTGAAGCCTA+TGG 0.510196 2:-23643685 None:intergenic
CAAGCTATTCGCTATAAGAT+CGG 0.514873 2:-23643943 None:intergenic
GCTTCAGTTTCAACAAGGGT+AGG 0.518211 2:+23644449 MsG0280007953.01.T01:CDS
GGTGTTCACAAATGATAAAG+AGG 0.524145 2:+23644598 MsG0280007953.01.T01:CDS
ATGATCTTAGTTGTTTGCAT+GGG 0.527005 2:+23643624 MsG0280007953.01.T01:CDS
GAGTTTGCTTAGAATGGTGG+TGG 0.529737 2:+23644309 MsG0280007953.01.T01:CDS
CGCATCTATTTACGTAGCAA+AGG 0.530451 2:+23643978 MsG0280007953.01.T01:CDS
GTAGGGAATGCACTTGGAGC+TGG 0.530874 2:+23644467 MsG0280007953.01.T01:CDS
CTAGTTGTGGAATGCTTAGA+GGG 0.539611 2:+23644690 MsG0280007953.01.T01:CDS
CGTGTAGCTTTAGCCACGGC+GGG 0.541296 2:+23644374 MsG0280007953.01.T01:CDS
TTCAGGTCTAGCCATGGGAA+TGG 0.547464 2:+23643722 MsG0280007953.01.T01:CDS
GTCCTTTCAGGTCTAGCCAT+GGG 0.551373 2:+23643717 MsG0280007953.01.T01:CDS
AGGTTAAGAAAATGATGATA+GGG 0.552617 2:-23644052 None:intergenic
AAGCTACACGAGGGTTGTAA+AGG 0.553073 2:-23644361 None:intergenic
CTTCAGTTTCAACAAGGGTA+GGG 0.553668 2:+23644450 MsG0280007953.01.T01:CDS
TGTGGTATATAACACTGATT+GGG 0.556810 2:+23644874 MsG0280007953.01.T01:CDS
GAACAACTTGGCCATTATTG+TGG 0.558363 2:+23644000 MsG0280007953.01.T01:CDS
TGTGGTATCAATTCTTGTTG+TGG 0.558880 2:+23644856 MsG0280007953.01.T01:CDS
GACTGAGTAACCGGTTACAT+TGG 0.562773 2:-23643697 None:intergenic
GGACGAGCTAAAGAAAATGA+TGG 0.566710 2:+23643551 MsG0280007953.01.T01:CDS
CGCCAATAGGAATAGTGTTG+GGG 0.568084 2:+23644771 MsG0280007953.01.T01:CDS
TTCTTGTTGTGATGATAACA+AGG 0.569941 2:-23644213 None:intergenic
CTTAGAGGGAGTGCAAGGCC+AGG 0.570090 2:+23644704 MsG0280007953.01.T01:CDS
GAGTTCATGACAATTCTAGC+TGG 0.573693 2:+23644335 MsG0280007953.01.T01:CDS
CTAAAGAAAATGATGGACAT+AGG 0.574782 2:+23643558 MsG0280007953.01.T01:CDS
CAAGGCCAGGTATTGGGGCA+GGG 0.575030 2:+23644717 MsG0280007953.01.T01:CDS
TTGGAGTTTGCTTAGAATGG+TGG 0.575348 2:+23644306 MsG0280007953.01.T01:CDS
CCTCTATGTACTCAAGCCAT+TGG 0.575821 2:+23643747 MsG0280007953.01.T01:CDS
TGTCCACAAACAACTAGTTG+TGG 0.580606 2:+23644677 MsG0280007953.01.T01:CDS
GGCGCCAATAGGAATAGTGT+TGG 0.580799 2:+23644769 MsG0280007953.01.T01:CDS
TATCGTACCTCAGCAGTTGT+CGG 0.582160 2:-23643113 None:intergenic
ACAAGGGTAGGGAATGCACT+TGG 0.586849 2:+23644461 MsG0280007953.01.T01:CDS
TTAACCTTCAAACTTCACCT+TGG 0.590547 2:+23644068 MsG0280007953.01.T01:CDS
TTGGGAAAGAGAGTCATTGA+AGG 0.590950 2:+23644892 MsG0280007953.01.T01:CDS
TACGTAGCAAAGGAACAACT+TGG 0.600971 2:+23643988 MsG0280007953.01.T01:CDS
AGTCCTTTCAGGTCTAGCCA+TGG 0.601305 2:+23643716 MsG0280007953.01.T01:CDS
CCCTCGTGTAGCTTTAGCCA+CGG 0.602755 2:+23644370 MsG0280007953.01.T01:CDS
AAGCACCACTTGCTAACTCA+AGG 0.604905 2:-23643659 None:intergenic
GGGAGGACTAGGGATAGGTA+TGG 0.606479 2:-23644192 None:intergenic
GAATGCTTAGAGGGAGTGCA+AGG 0.609636 2:+23644699 MsG0280007953.01.T01:CDS
CCAACAAGTGTCAAAACCCT+AGG 0.611672 2:+23644239 MsG0280007953.01.T01:CDS
TTCCCATGGCTAGACCTGAA+AGG 0.612296 2:-23643719 None:intergenic
ACATAGAGGCTCCATTCCCA+TGG 0.614544 2:-23643733 None:intergenic
TGGAGCTGGTCAACCAACAA+AGG 0.621113 2:+23644481 MsG0280007953.01.T01:CDS
CAAACAGTCAAATGTGAGCA+AGG 0.623309 2:+23644971 MsG0280007953.01.T01:CDS
CCAATGGCTTGAGTACATAG+AGG 0.624591 2:-23643747 None:intergenic
ACTTATGTACACTTTACCGA+CGG 0.625353 2:+23644418 MsG0280007953.01.T01:CDS
ATGATAACAAGGGAGGACTA+GGG 0.629298 2:-23644202 None:intergenic
GGAAGCCTTGAGTTAGCAAG+TGG 0.631750 2:+23643654 MsG0280007953.01.T01:CDS
AACAAGGGAGGACTAGGGAT+AGG 0.632327 2:-23644197 None:intergenic
TTGTATTACTATTCCCGCCG+TGG 0.632361 2:-23644387 None:intergenic
TGTTGTGATGATAACAAGGG+AGG 0.642183 2:-23644209 None:intergenic
GGTGTTAGAGTTGACAATGG+CGG 0.648706 2:+23644619 MsG0280007953.01.T01:CDS
TCTTGTTGTGATGATAACAA+GGG 0.654076 2:-23644212 None:intergenic
TTAGTGCTTCAGTTTCAACA+AGG 0.654495 2:+23644444 MsG0280007953.01.T01:CDS
ACTGAAGCACTAAGTGCCGT+CGG 0.658180 2:-23644434 None:intergenic
GACATACCCGACAACTGCTG+AGG 0.660676 2:+23643106 MsG0280007953.01.T01:CDS
CTATAAGATCGGGAATCGAG+AGG 0.665968 2:-23643932 None:intergenic
GGCAGCACAAATAGCATGTG+TGG 0.671756 2:+23644838 MsG0280007953.01.T01:CDS
TAGTGCTTCAGTTTCAACAA+GGG 0.675500 2:+23644445 MsG0280007953.01.T01:CDS
GCCGTGGCTAAAGCTACACG+AGG 0.684997 2:-23644371 None:intergenic
TAAGATCGGGAATCGAGAGG+CGG 0.690184 2:-23643929 None:intergenic
AAAACCCTAGGCAAAGAATG+GGG 0.698680 2:+23644251 MsG0280007953.01.T01:CDS
AGAGGTGTTAGAGTTGACAA+TGG 0.702435 2:+23644616 MsG0280007953.01.T01:CDS
TCGTGTAGCTTTAGCCACGG+CGG 0.710232 2:+23644373 MsG0280007953.01.T01:CDS
TAGCAATACCTTGTACACCA+AGG 0.710910 2:-23644085 None:intergenic
CCGTGGCTAAAGCTACACGA+GGG 0.768897 2:-23644370 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTTTACTCATTTTACT+TGG + Chr2:23644742-23644761 MsG0280007953.01.T01:CDS 15.0%
!!! CTATGATAATTTTTTTTTGT+TGG - Chr2:23643393-23643412 None:intergenic 15.0%
!! TTATGATATTGATGAAGAAA+TGG - Chr2:23643358-23643377 None:intergenic 20.0%
!!! TGTTTTTGTTTTGTTTTTTC+AGG + Chr2:23643524-23643543 MsG0280007953.01.T01:intron 20.0%
! AAGGTTAAGAAAATGATGAT+AGG - Chr2:23644056-23644075 None:intergenic 25.0%
! ACTATGAGTATATAGTATGA+GGG - Chr2:23643196-23643215 None:intergenic 25.0%
! ACTCATAGTATATACTTACA+TGG + Chr2:23643207-23643226 MsG0280007953.01.T01:intron 25.0%
! AGGTTAAGAAAATGATGATA+GGG - Chr2:23644055-23644074 None:intergenic 25.0%
! ATGAAAAATGTGAAAACCAT+GGG - Chr2:23643156-23643175 None:intergenic 25.0%
! GAAATGGTATATGAAAACTA+TGG - Chr2:23643342-23643361 None:intergenic 25.0%
! GCATAGCATATATATTAACA+TGG + Chr2:23643412-23643431 MsG0280007953.01.T01:intron 25.0%
! GTATGATAAAAGGAAGAAAA+GGG - Chr2:23644138-23644157 None:intergenic 25.0%
! TACTATGAGTATATAGTATG+AGG - Chr2:23643197-23643216 None:intergenic 25.0%
! TATGAAAAATGTGAAAACCA+TGG - Chr2:23643157-23643176 None:intergenic 25.0%
! TGTAGAACATGTATGATAAA+AGG - Chr2:23644148-23644167 None:intergenic 25.0%
! TGTATGATAAAAGGAAGAAA+AGG - Chr2:23644139-23644158 None:intergenic 25.0%
!! TTTTACTCATTTTACTTGGT+GGG + Chr2:23644746-23644765 MsG0280007953.01.T01:CDS 25.0%
!! TTTTTACTCATTTTACTTGG+TGG + Chr2:23644745-23644764 MsG0280007953.01.T01:CDS 25.0%
!!! TATGTCTTGATTTTGATGAA+GGG - Chr2:23643886-23643905 None:intergenic 25.0%
!!! TTATGTCTTGATTTTGATGA+AGG - Chr2:23643887-23643906 None:intergenic 25.0%
!!! TTTTGTTTTGTTTTTTCAGG+TGG + Chr2:23643527-23643546 MsG0280007953.01.T01:intron 25.0%
AAAGGCAAATTTGTCAACTA+TGG + Chr2:23644499-23644518 MsG0280007953.01.T01:CDS 30.0%
AACATTAGGGAGAGAAAAAT+GGG + Chr2:23644571-23644590 MsG0280007953.01.T01:CDS 30.0%
AACTCACAAACACCAATTAT+AGG - Chr2:23644650-23644669 None:intergenic 30.0%
ACTCTATTATTGGAGAAAGA+TGG + Chr2:23643452-23643471 MsG0280007953.01.T01:intron 30.0%
ATGATCTTAGTTGTTTGCAT+GGG + Chr2:23643624-23643643 MsG0280007953.01.T01:CDS 30.0%
CTCTATTATTGGAGAAAGAT+GGG + Chr2:23643453-23643472 MsG0280007953.01.T01:intron 30.0%
GATAGGGATATGAATGATAA+CGG - Chr2:23644039-23644058 None:intergenic 30.0%
GGTTATTATGGACAACATTA+GGG + Chr2:23644558-23644577 MsG0280007953.01.T01:CDS 30.0%
GTTCAACATTAGGGTTATTA+TGG + Chr2:23644546-23644565 MsG0280007953.01.T01:CDS 30.0%
TCTATTATTGGAGAAAGATG+GGG + Chr2:23643454-23643473 MsG0280007953.01.T01:intron 30.0%
TTGTGGTATATAACACTGAT+TGG + Chr2:23644873-23644892 MsG0280007953.01.T01:CDS 30.0%
! TCTTGTTGTGATGATAACAA+GGG - Chr2:23644215-23644234 None:intergenic 30.0%
! TGTGGTATATAACACTGATT+GGG + Chr2:23644874-23644893 MsG0280007953.01.T01:CDS 30.0%
! TTCTTGTTGTGATGATAACA+AGG - Chr2:23644216-23644235 None:intergenic 30.0%
! TTTTCCATTCAAAGTTGTCT+TGG + Chr2:23644287-23644306 MsG0280007953.01.T01:CDS 30.0%
!! CAACTAAGATCATGTTTTTG+AGG - Chr2:23643617-23643636 None:intergenic 30.0%
!! CTAAAGAAAATGATGGACAT+AGG + Chr2:23643558-23643577 MsG0280007953.01.T01:CDS 30.0%
!! GAAGAAAAGGGTATTGAAAT+TGG - Chr2:23644126-23644145 None:intergenic 30.0%
!! TTTACTCATTTTACTTGGTG+GGG + Chr2:23644747-23644766 MsG0280007953.01.T01:CDS 30.0%
!!! GTTTGTTTTGAAATTAGGGT+TGG + Chr2:23644793-23644812 MsG0280007953.01.T01:CDS 30.0%
!!! TGGGGTTTGTTTTGAAATTA+GGG + Chr2:23644789-23644808 MsG0280007953.01.T01:CDS 30.0%
!!! TTGGGGTTTGTTTTGAAATT+AGG + Chr2:23644788-23644807 MsG0280007953.01.T01:CDS 30.0%
AACTCCAAGACAACTTTGAA+TGG - Chr2:23644294-23644313 None:intergenic 35.0%
AAGAATGGGGCATGTTAATA+AGG + Chr2:23644264-23644283 MsG0280007953.01.T01:CDS 35.0%
AAGCTATTCGCTATAAGATC+GGG - Chr2:23643945-23643964 None:intergenic 35.0%
ACATTAGGGAGAGAAAAATG+GGG + Chr2:23644572-23644591 MsG0280007953.01.T01:CDS 35.0%
ACTTATGTACACTTTACCGA+CGG + Chr2:23644418-23644437 MsG0280007953.01.T01:CDS 35.0%
AGTGAAGTACACCACAATAA+TGG - Chr2:23644014-23644033 None:intergenic 35.0%
ATACCATACAAAATCTCCCA+TGG + Chr2:23643137-23643156 MsG0280007953.01.T01:intron 35.0%
ATTAAGCCACAAGAGTGAAA+TGG - Chr2:23643844-23643863 None:intergenic 35.0%
ATTCCACAACTAGTTGTTTG+TGG - Chr2:23644683-23644702 None:intergenic 35.0%
CAACATTAGGGAGAGAAAAA+TGG + Chr2:23644570-23644589 MsG0280007953.01.T01:CDS 35.0%
CAAGCTATTCGCTATAAGAT+CGG - Chr2:23643946-23643965 None:intergenic 35.0%
CACTAGCAAGTTCAACATTA+GGG + Chr2:23644537-23644556 MsG0280007953.01.T01:CDS 35.0%
CATGATCTTAGTTGTTTGCA+TGG + Chr2:23643623-23643642 MsG0280007953.01.T01:CDS 35.0%
CTACGTAAATAGATGCGAAT+TGG - Chr2:23643975-23643994 None:intergenic 35.0%
CTTTCTCCAATAATAGAGTG+AGG - Chr2:23643451-23643470 None:intergenic 35.0%
GGGTTATTATGGACAACATT+AGG + Chr2:23644557-23644576 MsG0280007953.01.T01:CDS 35.0%
GGTGTTCACAAATGATAAAG+AGG + Chr2:23644598-23644617 MsG0280007953.01.T01:CDS 35.0%
GTTGACAAATTTGCCTTTGT+TGG - Chr2:23644497-23644516 None:intergenic 35.0%
TAGTGCTTCAGTTTCAACAA+GGG + Chr2:23644445-23644464 MsG0280007953.01.T01:CDS 35.0%
TCACTAGCAAGTTCAACATT+AGG + Chr2:23644536-23644555 MsG0280007953.01.T01:CDS 35.0%
TGTGGTATCAATTCTTGTTG+TGG + Chr2:23644856-23644875 MsG0280007953.01.T01:CDS 35.0%
TGTTATAGCCGACCTTTAAT+CGG + Chr2:23643293-23643312 MsG0280007953.01.T01:intron 35.0%
TTAACCTTCAAACTTCACCT+TGG + Chr2:23644068-23644087 MsG0280007953.01.T01:CDS 35.0%
TTAAGCCACAAGAGTGAAAT+GGG - Chr2:23643843-23643862 None:intergenic 35.0%
TTAGTGCTTCAGTTTCAACA+AGG + Chr2:23644444-23644463 MsG0280007953.01.T01:CDS 35.0%
TTGTCAACTATGGTAGCAAT+TGG + Chr2:23644509-23644528 MsG0280007953.01.T01:CDS 35.0%
! AAACCATGGGAGATTTTGTA+TGG - Chr2:23643143-23643162 None:intergenic 35.0%
! ATGGCGGTTTTACCTATAAT+TGG + Chr2:23644635-23644654 MsG0280007953.01.T01:CDS 35.0%
!! AGAGAAGTTTTGTGAACCAA+TGG - Chr2:23643766-23643785 None:intergenic 35.0%
!! TTACTCATTTTACTTGGTGG+GGG + Chr2:23644748-23644767 MsG0280007953.01.T01:CDS 35.0%
!!! GTTTTGAAATTAGGGTTGGT+GGG + Chr2:23644797-23644816 MsG0280007953.01.T01:CDS 35.0%
!!! TGTTTTGAAATTAGGGTTGG+TGG + Chr2:23644796-23644815 MsG0280007953.01.T01:CDS 35.0%
!!! TGTTTTGTTTTTTCAGGTGG+TGG + Chr2:23643530-23643549 MsG0280007953.01.T01:intron 35.0%
!!! TTTTGAAATTAGGGTTGGTG+GGG + Chr2:23644798-23644817 MsG0280007953.01.T01:CDS 35.0%
AAAACCCTAGGCAAAGAATG+GGG + Chr2:23644251-23644270 MsG0280007953.01.T01:CDS 40.0%
AACCCCAACACTATTCCTAT+TGG - Chr2:23644776-23644795 None:intergenic 40.0%
AATAGAGTGAGGCTGCATAA+AGG - Chr2:23643440-23643459 None:intergenic 40.0%
ACTAGTTGTGGAATGCTTAG+AGG + Chr2:23644689-23644708 MsG0280007953.01.T01:CDS 40.0%
AGTTGTTTGCATGGGAAAGT+TGG + Chr2:23643632-23643651 MsG0280007953.01.T01:CDS 40.0%
ATAGGCTTCACCAATGTAAC+CGG + Chr2:23643687-23643706 MsG0280007953.01.T01:CDS 40.0%
ATGATAACAAGGGAGGACTA+GGG - Chr2:23644205-23644224 None:intergenic 40.0%
ATGCAGCCTCACTCTATTAT+TGG + Chr2:23643442-23643461 MsG0280007953.01.T01:intron 40.0%
ATTGAAGGCAAAAAGCTTGG+TGG + Chr2:23644907-23644926 MsG0280007953.01.T01:CDS 40.0%
CAAAACCCTAGGCAAAGAAT+GGG + Chr2:23644250-23644269 MsG0280007953.01.T01:CDS 40.0%
CAAACAGTCAAATGTGAGCA+AGG + Chr2:23644971-23644990 MsG0280007953.01.T01:CDS 40.0%
CATTACCCATTTCACTCTTG+TGG + Chr2:23643835-23643854 MsG0280007953.01.T01:CDS 40.0%
CATTAGGGAGAGAAAAATGG+GGG + Chr2:23644573-23644592 MsG0280007953.01.T01:CDS 40.0%
CGCATCTATTTACGTAGCAA+AGG + Chr2:23643978-23643997 MsG0280007953.01.T01:CDS 40.0%
CTAGTTGTGGAATGCTTAGA+GGG + Chr2:23644690-23644709 MsG0280007953.01.T01:CDS 40.0%
CTTCAGTTTCAACAAGGGTA+GGG + Chr2:23644450-23644469 MsG0280007953.01.T01:CDS 40.0%
CTTGCTCACATTTGACTGTT+TGG - Chr2:23644973-23644992 None:intergenic 40.0%
GAACAACTTGGCCATTATTG+TGG + Chr2:23644000-23644019 MsG0280007953.01.T01:CDS 40.0%
GAGTTCATGACAATTCTAGC+TGG + Chr2:23644335-23644354 MsG0280007953.01.T01:CDS 40.0%
GGACGAGCTAAAGAAAATGA+TGG + Chr2:23643551-23643570 MsG0280007953.01.T01:CDS 40.0%
GTCATTGAAGGCAAAAAGCT+TGG + Chr2:23644904-23644923 MsG0280007953.01.T01:CDS 40.0%
GTTGTTTGCATGGGAAAGTT+GGG + Chr2:23643633-23643652 MsG0280007953.01.T01:CDS 40.0%
TACACCAAGGTGAAGTTTGA+AGG - Chr2:23644075-23644094 None:intergenic 40.0%
TACGTAGCAAAGGAACAACT+TGG + Chr2:23643988-23644007 MsG0280007953.01.T01:CDS 40.0%
TAGCAATACCTTGTACACCA+AGG - Chr2:23644088-23644107 None:intergenic 40.0%
TCAAAACCCTAGGCAAAGAA+TGG + Chr2:23644249-23644268 MsG0280007953.01.T01:CDS 40.0%
TGATGAAGGGATAGCATGAA+TGG - Chr2:23643873-23643892 None:intergenic 40.0%
TGTCCACAAACAACTAGTTG+TGG + Chr2:23644677-23644696 MsG0280007953.01.T01:CDS 40.0%
TGTTGTGATGATAACAAGGG+AGG - Chr2:23644212-23644231 None:intergenic 40.0%
TTGAAGGCAAAAAGCTTGGT+GGG + Chr2:23644908-23644927 MsG0280007953.01.T01:CDS 40.0%
TTGGGAAAGAGAGTCATTGA+AGG + Chr2:23644892-23644911 MsG0280007953.01.T01:CDS 40.0%
! AAACTTCACCTTGGTGTACA+AGG + Chr2:23644077-23644096 MsG0280007953.01.T01:CDS 40.0%
! AGTTGTCGGGTATGTCTTTT+GGG - Chr2:23643102-23643121 None:intergenic 40.0%
! GTCTTGGAGTTTGCTTAGAA+TGG + Chr2:23644303-23644322 MsG0280007953.01.T01:CDS 40.0%
! TTGGAGTTTGCTTAGAATGG+TGG + Chr2:23644306-23644325 MsG0280007953.01.T01:CDS 40.0%
!! AGAGGTGTTAGAGTTGACAA+TGG + Chr2:23644616-23644635 MsG0280007953.01.T01:CDS 40.0%
!! AGCAGTTGATGACATTTGAC+CGG - Chr2:23643255-23643274 None:intergenic 40.0%
!!! GGGGTTTTGTTATGGTTTGT+TGG + Chr2:23644817-23644836 MsG0280007953.01.T01:CDS 40.0%
AAGCTACACGAGGGTTGTAA+AGG - Chr2:23644364-23644383 None:intergenic 45.0%
ACCTGAAAGGACTGAGTAAC+CGG - Chr2:23643709-23643728 None:intergenic 45.0%
ACTATGCTCATGGCTGCTAT+TGG - Chr2:23643588-23643607 None:intergenic 45.0%
ATAGCAGCCATGAGCATAGT+AGG + Chr2:23643588-23643607 MsG0280007953.01.T01:CDS 45.0%
ATAGCCGACCTTTAATCGGA+GGG + Chr2:23643297-23643316 MsG0280007953.01.T01:intron 45.0%
CCAACAAGTGTCAAAACCCT+AGG + Chr2:23644239-23644258 MsG0280007953.01.T01:CDS 45.0%
CCAATGGCTTGAGTACATAG+AGG - Chr2:23643750-23643769 None:intergenic 45.0%
CCTCTATGTACTCAAGCCAT+TGG + Chr2:23643747-23643766 MsG0280007953.01.T01:CDS 45.0%
CGCCAATAGGAATAGTGTTG+GGG + Chr2:23644771-23644790 MsG0280007953.01.T01:CDS 45.0%
CTATAAGATCGGGAATCGAG+AGG - Chr2:23643935-23643954 None:intergenic 45.0%
CTATGCTCATGGCTGCTATT+GGG - Chr2:23643587-23643606 None:intergenic 45.0%
GACTGAGTAACCGGTTACAT+TGG - Chr2:23643700-23643719 None:intergenic 45.0%
GATGATAACAAGGGAGGACT+AGG - Chr2:23644206-23644225 None:intergenic 45.0%
GCGCCAATAGGAATAGTGTT+GGG + Chr2:23644770-23644789 MsG0280007953.01.T01:CDS 45.0%
GCTTCAGTTTCAACAAGGGT+AGG + Chr2:23644449-23644468 MsG0280007953.01.T01:CDS 45.0%
GGTGTTAGAGTTGACAATGG+CGG + Chr2:23644619-23644638 MsG0280007953.01.T01:CDS 45.0%
GGTTACATTGGTGAAGCCTA+TGG - Chr2:23643688-23643707 None:intergenic 45.0%
GTTATGCTCCCTCCGATTAA+AGG - Chr2:23643308-23643327 None:intergenic 45.0%
TATAGCCGACCTTTAATCGG+AGG + Chr2:23643296-23643315 MsG0280007953.01.T01:intron 45.0%
TATCGTACCTCAGCAGTTGT+CGG - Chr2:23643116-23643135 None:intergenic 45.0%
TGAGTTAGCAAGTGGTGCTT+TGG + Chr2:23643662-23643681 MsG0280007953.01.T01:CDS 45.0%
TTGTATTACTATTCCCGCCG+TGG - Chr2:23644390-23644409 None:intergenic 45.0%
! AAGCACCACTTGCTAACTCA+AGG - Chr2:23643662-23643681 None:intergenic 45.0%
! CAGTTGTCGGGTATGTCTTT+TGG - Chr2:23643103-23643122 None:intergenic 45.0%
! GAGGTATCCTACTATGCTCA+TGG - Chr2:23643598-23643617 None:intergenic 45.0%
! GAGTTTGCTTAGAATGGTGG+TGG + Chr2:23644309-23644328 MsG0280007953.01.T01:CDS 45.0%
!! CCTAGGGTTTTGACACTTGT+TGG - Chr2:23644242-23644261 None:intergenic 45.0%
AACAAGGGAGGACTAGGGAT+AGG - Chr2:23644200-23644219 None:intergenic 50.0%
ACAAGGGTAGGGAATGCACT+TGG + Chr2:23644461-23644480 MsG0280007953.01.T01:CDS 50.0%
ACATAGAGGCTCCATTCCCA+TGG - Chr2:23643736-23643755 None:intergenic 50.0%
ACATGCCCCATTCTTTGCCT+AGG - Chr2:23644259-23644278 None:intergenic 50.0%
ACCGGTTACTCAGTCCTTTC+AGG + Chr2:23643705-23643724 MsG0280007953.01.T01:CDS 50.0%
AGGGAGAGAAAAATGGGGGA+AGG + Chr2:23644577-23644596 MsG0280007953.01.T01:CDS 50.0%
ATCGTACCTCAGCAGTTGTC+GGG - Chr2:23643115-23643134 None:intergenic 50.0%
CATGCCCCATTCTTTGCCTA+GGG - Chr2:23644258-23644277 None:intergenic 50.0%
GGAAGCCTTGAGTTAGCAAG+TGG + Chr2:23643654-23643673 MsG0280007953.01.T01:CDS 50.0%
GGCAGCACAAATAGCATGTG+TGG + Chr2:23644838-23644857 MsG0280007953.01.T01:CDS 50.0%
GGCGCCAATAGGAATAGTGT+TGG + Chr2:23644769-23644788 MsG0280007953.01.T01:CDS 50.0%
TAAGATCGGGAATCGAGAGG+CGG - Chr2:23643932-23643951 None:intergenic 50.0%
TGACATTTGACCGGAGAGAG+AGG - Chr2:23643246-23643265 None:intergenic 50.0%
TGCTCCCTCCGATTAAAGGT+CGG - Chr2:23643304-23643323 None:intergenic 50.0%
TGGAGCTGGTCAACCAACAA+AGG + Chr2:23644481-23644500 MsG0280007953.01.T01:CDS 50.0%
TTCAGGTCTAGCCATGGGAA+TGG + Chr2:23643722-23643741 MsG0280007953.01.T01:CDS 50.0%
TTCCCATGGCTAGACCTGAA+AGG - Chr2:23643722-23643741 None:intergenic 50.0%
TTTATCCCTGCCCCAATACC+TGG - Chr2:23644725-23644744 None:intergenic 50.0%
! ACTGAAGCACTAAGTGCCGT+CGG - Chr2:23644437-23644456 None:intergenic 50.0%
! AGTCCTTTCAGGTCTAGCCA+TGG + Chr2:23643716-23643735 MsG0280007953.01.T01:CDS 50.0%
! GAATGCTTAGAGGGAGTGCA+AGG + Chr2:23644699-23644718 MsG0280007953.01.T01:CDS 50.0%
! GTCCTTTCAGGTCTAGCCAT+GGG + Chr2:23643717-23643736 MsG0280007953.01.T01:CDS 50.0%
!!! GGGTTGGTGGGGTTTTGTTA+TGG + Chr2:23644809-23644828 MsG0280007953.01.T01:CDS 50.0%
CATTTGACCGGAGAGAGAGG+CGG - Chr2:23643243-23643262 None:intergenic 55.0%
CCGTGGCTAAAGCTACACGA+GGG - Chr2:23644373-23644392 None:intergenic 55.0%
GAATCGAGAGGCGGCAATAG+AGG - Chr2:23643923-23643942 None:intergenic 55.0%
GACATACCCGACAACTGCTG+AGG + Chr2:23643106-23643125 MsG0280007953.01.T01:CDS 55.0%
GCAAGTGGTGCTTTGGCCAT+AGG + Chr2:23643669-23643688 MsG0280007953.01.T01:CDS 55.0%
GGAGTGCAAGGCCAGGTATT+GGG + Chr2:23644711-23644730 MsG0280007953.01.T01:CDS 55.0%
GGGAGGACTAGGGATAGGTA+TGG - Chr2:23644195-23644214 None:intergenic 55.0%
GGTCGGCTATAACAGCTCTC+TGG - Chr2:23643287-23643306 None:intergenic 55.0%
GTAGGGAATGCACTTGGAGC+TGG + Chr2:23644467-23644486 MsG0280007953.01.T01:CDS 55.0%
! CTTTTTGCCGCCTCTCTCTC+CGG + Chr2:23643233-23643252 MsG0280007953.01.T01:intron 55.0%
! GAGTGCAAGGCCAGGTATTG+GGG + Chr2:23644712-23644731 MsG0280007953.01.T01:CDS 55.0%
!! CCCTCGTGTAGCTTTAGCCA+CGG + Chr2:23644370-23644389 MsG0280007953.01.T01:CDS 55.0%
!! TCGTGTAGCTTTAGCCACGG+CGG + Chr2:23644373-23644392 MsG0280007953.01.T01:CDS 55.0%
CGGAGAGAGAGGCGGCAAAA+AGG - Chr2:23643235-23643254 None:intergenic 60.0%
GCCGTGGCTAAAGCTACACG+AGG - Chr2:23644374-23644393 None:intergenic 60.0%
! CTTAGAGGGAGTGCAAGGCC+AGG + Chr2:23644704-23644723 MsG0280007953.01.T01:CDS 60.0%
! GGGAGTGCAAGGCCAGGTAT+TGG + Chr2:23644710-23644729 MsG0280007953.01.T01:CDS 60.0%
!! CAAGGCCAGGTATTGGGGCA+GGG + Chr2:23644717-23644736 MsG0280007953.01.T01:CDS 60.0%
!! CGTGTAGCTTTAGCCACGGC+GGG + Chr2:23644374-23644393 MsG0280007953.01.T01:CDS 60.0%
!! TACTTGGTGGGGGCGCCAAT+AGG + Chr2:23644758-23644777 MsG0280007953.01.T01:CDS 60.0%
!! GCAAGGCCAGGTATTGGGGC+AGG + Chr2:23644716-23644735 MsG0280007953.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 23643080 23645021 23643080 ID=MsG0280007953.01;Name=MsG0280007953.01
Chr2 mRNA 23643080 23645021 23643080 ID=MsG0280007953.01.T01;Parent=MsG0280007953.01;Name=MsG0280007953.01.T01;_AED=0.36;_eAED=0.36;_QI=0|0|0|1|1|1|2|0|507
Chr2 exon 23643080 23643127 23643080 ID=MsG0280007953.01.T01:exon:18532;Parent=MsG0280007953.01.T01
Chr2 exon 23643546 23645021 23643546 ID=MsG0280007953.01.T01:exon:18533;Parent=MsG0280007953.01.T01
Chr2 CDS 23643080 23643127 23643080 ID=MsG0280007953.01.T01:cds;Parent=MsG0280007953.01.T01
Chr2 CDS 23643546 23645021 23643546 ID=MsG0280007953.01.T01:cds;Parent=MsG0280007953.01.T01
Gene Sequence

>MsG0280007953.01.T01

ATGGTTGCTCAAGAGAAATCCCAAAAGACATACCCGACAACTGCTGAGGTGGTGGACGAGCTAAAGAAAATGATGGACATAGGTTTCCCAATAGCAGCCATGAGCATAGTAGGATACCTCAAAAACATGATCTTAGTTGTTTGCATGGGAAAGTTGGGAAGCCTTGAGTTAGCAAGTGGTGCTTTGGCCATAGGCTTCACCAATGTAACCGGTTACTCAGTCCTTTCAGGTCTAGCCATGGGAATGGAGCCTCTATGTACTCAAGCCATTGGTTCACAAAACTTCTCTTTAGTCTCTCTCATATTAAGAAGAACAATTCTCATGTTATTAGTAGCTTCATTACCCATTTCACTCTTGTGGCTTAATCTTGAACCATTCATGCTATCCCTTCATCAAAATCAAGACATAACAAGAATAGCAAGCCTCTATTGCCGCCTCTCGATTCCCGATCTTATAGCGAATAGCTTGCTTCATCCAATTCGCATCTATTTACGTAGCAAAGGAACAACTTGGCCATTATTGTGGTGTACTTCACTTTCCGTTATCATTCATATCCCTATCATCATTTTCTTAACCTTCAAACTTCACCTTGGTGTACAAGGTATTGCTATTTCAGCTTTTGTTGCCAATTTCAATACCCTTTTCTTCCTTTTATCATACATGTTCTACATGCATGTCTCACATGTTTCAATCTCCATACCTATCCCTAGTCCTCCCTTGTTATCATCACAACAAGAAAAACCAACAAGTGTCAAAACCCTAGGCAAAGAATGGGGCATGTTAATAAGGTTTTCCATTCAAAGTTGTCTTGGAGTTTGCTTAGAATGGTGGTGGTATGAGTTCATGACAATTCTAGCTGGTTACCTTTACAACCCTCGTGTAGCTTTAGCCACGGCGGGAATAGTAATACAAACAACATCACTTATGTACACTTTACCGACGGCACTTAGTGCTTCAGTTTCAACAAGGGTAGGGAATGCACTTGGAGCTGGTCAACCAACAAAGGCAAATTTGTCAACTATGGTAGCAATTGGAATGTCACTAGCAAGTTCAACATTAGGGTTATTATGGACAACATTAGGGAGAGAAAAATGGGGGAAGGTGTTCACAAATGATAAAGAGGTGTTAGAGTTGACAATGGCGGTTTTACCTATAATTGGTGTTTGTGAGTTAGCAAATTGTCCACAAACAACTAGTTGTGGAATGCTTAGAGGGAGTGCAAGGCCAGGTATTGGGGCAGGGATAAATTTTTACTCATTTTACTTGGTGGGGGCGCCAATAGGAATAGTGTTGGGGTTTGTTTTGAAATTAGGGTTGGTGGGGTTTTGTTATGGTTTGTTGGCAGCACAAATAGCATGTGTGGTATCAATTCTTGTTGTGGTATATAACACTGATTGGGAAAGAGAGTCATTGAAGGCAAAAAGCTTGGTGGGTAATGATACATGTGACACATTATTTGCTCATGTTGAAGACCAAACAGTCAAATGTGAGCAAGGTATTGTCTTTCTCAATGAGAACAAGTGA
Protein sequence

>MsG0280007953.01.T01

MVAQEKSQKTYPTTAEVVDELKKMMDIGFPIAAMSIVGYLKNMILVVCMGKLGSLELASGALAIGFTNVTGYSVLSGLAMGMEPLCTQAIGSQNFSLVSLILRRTILMLLVASLPISLLWLNLEPFMLSLHQNQDITRIASLYCRLSIPDLIANSLLHPIRIYLRSKGTTWPLLWCTSLSVIIHIPIIIFLTFKLHLGVQGIAISAFVANFNTLFFLLSYMFYMHVSHVSISIPIPSPPLLSSQQEKPTSVKTLGKEWGMLIRFSIQSCLGVCLEWWWYEFMTILAGYLYNPRVALATAGIVIQTTSLMYTLPTALSASVSTRVGNALGAGQPTKANLSTMVAIGMSLASSTLGLLWTTLGREKWGKVFTNDKEVLELTMAVLPIIGVCELANCPQTTSCGMLRGSARPGIGAGINFYSFYLVGAPIGIVLGFVLKLGLVGFCYGLLAAQIACVVSILVVVYNTDWERESLKAKSLVGNDTCDTLFAHVEDQTVKCEQGIVFLNENK*