AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024415.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024415.01.T01 MTR_5g010830 99.258 539 2 2 1 537 1 539 0.0 1075
MsG0580024415.01.T01 MTR_1g101500 67.351 536 155 5 1 521 1 531 0.0 705
MsG0580024415.01.T01 MTR_0039s0080 66.085 516 154 4 16 526 3 502 0.0 671
MsG0580024415.01.T01 MTR_5g067460 57.529 518 211 2 1 518 1 509 0.0 610
MsG0580024415.01.T01 MTR_3g111020 55.827 532 208 5 32 536 13 544 0.0 588
MsG0580024415.01.T01 MTR_6g088805 53.066 473 219 1 47 519 10 479 0.0 526
MsG0580024415.01.T01 MTR_8g102430 52.632 456 196 2 51 506 10 445 4.69e-173 496
MsG0580024415.01.T01 MTR_4g027920 52.371 464 218 2 64 524 156 619 5.10e-168 490
MsG0580024415.01.T01 MTR_6g043810 48.049 487 235 5 47 518 6 489 9.12e-161 467
MsG0580024415.01.T01 MTR_6g043810 48.049 487 235 5 47 518 16 499 1.74e-160 467
MsG0580024415.01.T01 MTR_2g435930 42.770 491 259 5 52 521 5 494 2.03e-133 397
MsG0580024415.01.T01 MTR_6g081410 34.483 464 302 1 52 513 13 476 6.98e-93 292
MsG0580024415.01.T01 MTR_5g011540 38.210 458 276 4 60 513 39 493 4.32e-91 288
MsG0580024415.01.T01 MTR_6g081810 33.263 475 285 3 42 513 5 450 1.31e-88 280
MsG0580024415.01.T01 MTR_8g106670 36.283 452 272 7 61 504 9 452 1.52e-87 278
MsG0580024415.01.T01 MTR_6g066380 33.753 477 308 3 42 513 5 478 5.60e-87 277
MsG0580024415.01.T01 MTR_6g081780 33.407 455 301 1 52 504 13 467 8.83e-87 276
MsG0580024415.01.T01 MTR_6g081400 33.626 455 293 3 64 513 15 465 2.52e-86 275
MsG0580024415.01.T01 MTR_6g081300 35.227 440 285 0 63 502 9 448 7.98e-86 273
MsG0580024415.01.T01 MTR_1g108840 32.895 456 300 2 52 504 29 481 1.04e-83 269
MsG0580024415.01.T01 MTR_1g108810 33.913 460 287 6 47 498 18 468 7.48e-82 263
MsG0580024415.01.T01 MTR_6g081240 34.325 437 285 2 63 498 9 444 2.94e-81 261
MsG0580024415.01.T01 MTR_6g081310 35.664 429 260 4 88 502 20 446 9.18e-80 258
MsG0580024415.01.T01 MTR_6g004220 32.700 474 298 8 39 504 4 464 7.59e-77 250
MsG0580024415.01.T01 MTR_2g078930 32.657 493 308 5 25 514 16 487 2.62e-76 249
MsG0580024415.01.T01 MTR_6g081390 31.674 442 301 1 64 504 12 453 7.25e-76 247
MsG0580024415.01.T01 MTR_7g082810 31.770 469 305 6 56 515 13 475 4.68e-74 243
MsG0580024415.01.T01 MTR_6g004220 32.135 473 300 8 34 498 6 465 1.20e-73 243
MsG0580024415.01.T01 MTR_2g079020 31.864 499 310 6 33 527 18 490 2.60e-73 242
MsG0580024415.01.T01 MTR_2g078970 32.484 471 300 5 61 527 28 484 8.70e-72 237
MsG0580024415.01.T01 MTR_2g088400 29.730 444 311 1 72 514 60 503 4.52e-71 236
MsG0580024415.01.T01 MTR_1g109060 33.406 458 295 4 63 515 23 475 9.07e-70 231
MsG0580024415.01.T01 MTR_2g078960 34.086 443 269 5 61 492 30 460 6.34e-68 227
MsG0580024415.01.T01 MTR_4g048000 28.222 450 322 1 65 513 65 514 3.05e-67 226
MsG0580024415.01.T01 MTR_8g069470 28.932 515 331 10 18 516 16 511 4.43e-67 226
MsG0580024415.01.T01 MTR_1g109060 33.030 439 288 2 63 498 23 458 4.81e-67 225
MsG0580024415.01.T01 MTR_1g108990 31.866 477 313 6 40 510 1 471 3.27e-66 222
MsG0580024415.01.T01 MTR_2g022980 29.099 433 307 0 72 504 56 488 2.56e-62 213
MsG0580024415.01.T01 MTR_2g088400 30.151 398 278 0 72 469 60 457 1.61e-61 209
MsG0580024415.01.T01 MTR_1g021740 30.112 445 298 5 65 503 36 473 1.65e-60 207
MsG0580024415.01.T01 MTR_7g082800 31.750 400 259 5 123 515 1 393 2.58e-59 202
MsG0580024415.01.T01 MTR_6g004220 33.077 390 240 8 34 415 6 382 4.18e-59 202
MsG0580024415.01.T01 MTR_5g032720 29.075 454 318 3 65 516 37 488 1.15e-57 200
MsG0580024415.01.T01 MTR_6g047610 27.984 486 337 4 40 517 1 481 1.48e-57 199
MsG0580024415.01.T01 MTR_8g063590 31.567 434 286 7 87 514 76 504 3.45e-57 199
MsG0580024415.01.T01 MTR_5g032750 30.108 465 314 6 47 503 14 475 4.29e-56 196
MsG0580024415.01.T01 MTR_1g100180 28.958 480 321 7 51 526 34 497 3.31e-55 194
MsG0580024415.01.T01 MTR_6g047640 28.747 487 314 6 40 517 1 463 5.27e-55 192
MsG0580024415.01.T01 MTR_7g087410 27.887 459 322 3 64 518 31 484 1.38e-54 192
MsG0580024415.01.T01 MTR_5g033100 26.719 509 347 7 18 519 21 510 3.74e-54 191
MsG0580024415.01.T01 MTR_5g090290 31.333 450 297 5 87 531 69 511 4.32e-54 192
MsG0580024415.01.T01 MTR_6g047640 29.158 487 331 5 40 517 1 482 5.15e-53 187
MsG0580024415.01.T01 MTR_6g047590 28.298 470 324 4 40 501 1 465 3.23e-52 185
MsG0580024415.01.T01 MTR_7g087400 26.696 457 325 4 64 516 30 480 2.89e-51 182
MsG0580024415.01.T01 MTR_3g099700 25.646 503 355 7 31 520 6 502 4.47e-49 177
MsG0580024415.01.T01 MTR_6g004220 33.538 325 201 6 34 354 6 319 4.68e-48 170
MsG0580024415.01.T01 MTR_7g087410 26.768 396 289 1 123 518 1 395 3.97e-47 169
MsG0580024415.01.T01 MTR_3g099720 24.795 488 359 3 34 514 4 490 8.33e-47 171
MsG0580024415.01.T01 MTR_6g027200 25.702 463 323 3 64 516 36 487 1.18e-45 167
MsG0580024415.01.T01 MTR_6g027190 26.569 478 322 6 53 516 13 475 1.06e-43 162
MsG0580024415.01.T01 MTR_7g087430 27.089 395 287 1 123 517 1 394 9.27e-43 157
MsG0580024415.01.T01 MTR_7g087370 24.528 477 321 5 44 516 10 451 1.64e-42 158
MsG0580024415.01.T01 MTR_2g078990 29.630 324 210 5 208 527 4 313 1.49e-37 141
MsG0580024415.01.T01 MTR_5g087930 28.715 397 260 4 81 476 43 417 3.26e-37 143
MsG0580024415.01.T01 MTR_5g090280 26.995 426 268 6 126 520 4 417 5.40e-26 110
MsG0580024415.01.T01 MTR_2g079030 32.159 227 140 5 303 527 319 533 1.33e-25 110
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024415.01.T01 AT4G23030 69.008 484 143 3 40 516 1 484 0.0 616
MsG0580024415.01.T01 AT1G58340 62.335 454 171 0 54 507 47 500 0.0 558
MsG0580024415.01.T01 AT4G29140 56.118 474 204 2 62 535 53 522 0.0 553
MsG0580024415.01.T01 AT5G52050 55.556 495 208 4 34 518 5 497 0.0 547
MsG0580024415.01.T01 AT5G19700 53.469 490 217 1 23 512 5 483 0.0 542
MsG0580024415.01.T01 AT2G38510 50.218 458 227 1 64 520 5 462 3.06e-169 488
MsG0580024415.01.T01 AT1G71870 46.735 490 243 3 47 518 6 495 1.93e-156 456
MsG0580024415.01.T01 AT5G49130 46.004 463 232 3 63 509 24 484 8.48e-134 398
MsG0580024415.01.T01 AT4G22790 40.851 470 264 10 56 518 21 483 1.33e-92 292
MsG0580024415.01.T01 AT1G71140 33.839 461 292 6 54 509 18 470 3.74e-82 265
MsG0580024415.01.T01 AT5G52450 33.874 493 299 7 39 522 11 485 8.26e-82 263
MsG0580024415.01.T01 AT2G04070 34.316 475 306 4 40 510 1 473 2.10e-81 262
MsG0580024415.01.T01 AT2G34360 34.016 488 290 10 37 514 5 470 6.41e-81 261
MsG0580024415.01.T01 AT1G73700 34.232 482 305 4 39 516 3 476 9.58e-81 260
MsG0580024415.01.T01 AT2G04080 33.262 469 306 3 40 504 1 466 3.61e-80 259
MsG0580024415.01.T01 AT2G04050 34.395 471 296 4 44 510 12 473 3.42e-78 254
MsG0580024415.01.T01 AT2G04080 32.903 465 305 3 40 500 1 462 9.58e-77 250
MsG0580024415.01.T01 AT1G15150 31.360 456 309 2 65 518 31 484 1.49e-75 247
MsG0580024415.01.T01 AT2G04040 32.506 443 293 2 65 504 27 466 1.52e-75 247
MsG0580024415.01.T01 AT1G15170 33.933 445 284 4 65 504 34 473 1.31e-74 244
MsG0580024415.01.T01 AT1G15160 31.590 478 319 3 47 518 9 484 2.75e-74 244
MsG0580024415.01.T01 AT2G04090 32.506 443 293 2 65 504 30 469 1.11e-72 239
MsG0580024415.01.T01 AT2G04100 32.506 443 293 2 65 504 30 469 1.82e-72 239
MsG0580024415.01.T01 AT1G66760 32.495 477 304 7 36 504 1 467 3.17e-72 238
MsG0580024415.01.T01 AT1G64820 31.590 478 306 6 61 523 25 496 5.56e-72 238
MsG0580024415.01.T01 AT1G61890 28.827 503 346 6 18 516 2 496 2.20e-71 237
MsG0580024415.01.T01 AT3G23550 33.260 454 292 3 47 497 18 463 7.72e-71 234
MsG0580024415.01.T01 AT2G04100 32.494 437 289 2 65 498 30 463 8.50e-70 231
MsG0580024415.01.T01 AT1G66760 32.272 471 301 7 36 498 1 461 8.50e-70 231
MsG0580024415.01.T01 AT1G66780 31.878 458 302 4 52 504 22 474 2.48e-68 228
MsG0580024415.01.T01 AT2G34360 31.134 485 266 8 37 514 5 428 3.89e-68 226
MsG0580024415.01.T01 AT1G11670 29.213 445 311 2 62 504 47 489 5.08e-68 228
MsG0580024415.01.T01 AT1G15180 31.799 478 316 4 31 504 3 474 5.23e-68 227
MsG0580024415.01.T01 AT1G61890 28.660 485 336 5 18 498 2 480 1.17e-67 227
MsG0580024415.01.T01 AT3G23560 34.845 419 263 5 63 476 37 450 3.16e-67 225
MsG0580024415.01.T01 AT3G21690 27.079 469 338 1 47 515 38 502 1.84e-64 218
MsG0580024415.01.T01 AT4G21910 27.701 509 342 5 24 513 2 503 7.38e-64 217
MsG0580024415.01.T01 AT4G21910 27.701 509 342 5 24 513 2 503 7.38e-64 217
MsG0580024415.01.T01 AT4G21910 29.111 450 317 2 65 513 57 505 2.16e-62 213
MsG0580024415.01.T01 AT4G21910 29.111 450 317 2 65 513 57 505 2.16e-62 213
MsG0580024415.01.T01 AT3G59030 29.630 459 315 2 61 515 47 501 4.70e-62 212
MsG0580024415.01.T01 AT3G26590 27.983 461 325 4 65 523 45 500 5.49e-62 212
MsG0580024415.01.T01 AT1G61890 30.402 398 277 0 65 462 47 444 1.80e-61 210
MsG0580024415.01.T01 AT1G15170 34.097 349 220 4 161 504 11 354 1.76e-59 201
MsG0580024415.01.T01 AT1G23300 28.235 510 339 9 18 518 5 496 5.08e-59 204
MsG0580024415.01.T01 AT5G38030 27.991 443 310 5 65 503 45 482 9.74e-59 203
MsG0580024415.01.T01 AT4G00350 26.233 507 362 4 13 518 44 539 3.71e-57 200
MsG0580024415.01.T01 AT4G21903 28.205 429 306 2 88 515 76 503 1.23e-56 197
MsG0580024415.01.T01 AT3G23560 34.682 346 219 3 63 405 37 378 4.91e-56 194
MsG0580024415.01.T01 AT1G12950 27.578 446 310 5 65 504 67 505 5.13e-56 196
MsG0580024415.01.T01 AT5G10420 31.140 456 302 5 65 512 38 489 1.22e-55 194
MsG0580024415.01.T01 AT3G26590 27.595 395 281 3 126 518 4 395 1.78e-55 192
MsG0580024415.01.T01 AT1G47530 26.316 475 337 6 37 503 3 472 2.84e-55 193
MsG0580024415.01.T01 AT1G15150 30.833 360 245 2 161 518 11 368 3.73e-55 190
MsG0580024415.01.T01 AT4G21903 28.571 392 279 1 126 516 4 395 3.94e-54 188
MsG0580024415.01.T01 AT5G44050 30.512 449 292 8 65 503 40 478 7.35e-54 190
MsG0580024415.01.T01 AT4G21903 27.703 444 308 4 88 531 76 506 1.44e-53 189
MsG0580024415.01.T01 AT1G66760 30.923 401 263 5 36 430 1 393 2.58e-52 183
MsG0580024415.01.T01 AT5G65380 30.258 466 306 9 47 502 18 474 3.35e-52 185
MsG0580024415.01.T01 AT1G33090 30.193 467 304 8 65 517 36 494 2.89e-51 183
MsG0580024415.01.T01 AT4G25640 29.011 455 317 2 64 514 33 485 2.99e-51 182
MsG0580024415.01.T01 AT4G25640 29.011 455 317 2 64 514 33 485 4.83e-51 182
MsG0580024415.01.T01 AT1G33100 28.726 463 316 6 65 517 33 491 1.93e-50 181
MsG0580024415.01.T01 AT1G33110 28.205 468 322 8 39 498 7 468 6.02e-49 176
MsG0580024415.01.T01 AT1G33080 26.767 467 334 4 37 498 5 468 1.32e-47 173
MsG0580024415.01.T01 AT1G33080 26.767 467 334 4 37 498 5 468 2.92e-47 172
MsG0580024415.01.T01 AT3G59030 30.749 374 251 2 61 430 47 416 1.36e-46 168
MsG0580024415.01.T01 AT1G47530 26.440 382 272 5 127 504 5 381 5.26e-46 166
MsG0580024415.01.T01 AT5G17700 26.105 475 333 9 39 504 7 472 5.58e-45 166
MsG0580024415.01.T01 AT5G17700 26.105 475 333 9 39 504 7 472 5.58e-45 166
MsG0580024415.01.T01 AT3G03620 26.596 470 330 7 64 526 36 497 1.25e-43 162
MsG0580024415.01.T01 AT3G03620 26.596 470 330 7 64 526 36 497 1.25e-43 162
MsG0580024415.01.T01 AT4G25640 28.784 403 285 1 64 466 33 433 4.51e-43 160
MsG0580024415.01.T01 AT1G66760 31.563 339 216 6 168 498 3 333 1.82e-41 152
MsG0580024415.01.T01 AT1G33110 27.569 399 279 6 39 431 7 401 1.67e-34 135
MsG0580024415.01.T01 AT1G15180 32.308 260 170 2 161 417 11 267 3.17e-34 134
MsG0580024415.01.T01 AT5G17700 25.978 358 251 7 39 388 7 358 1.19e-29 121
MsG0580024415.01.T01 AT2G04066 32.258 124 82 1 381 504 40 161 2.88e-14 71.2

Find 94 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATTTACATTTGGGTTTC+TGG 0.044651 5:+4593301 MsG0580024415.01.T01:CDS
AGGTTTGTGGTTTGGTCTTA+TGG 0.195458 5:+4593921 MsG0580024415.01.T01:CDS
TTTCCTTCTTCTTTGAGTTT+TGG 0.208924 5:+4593535 MsG0580024415.01.T01:CDS
TGTTTGAATGTTGGGGATTT+AGG 0.209468 5:-4592650 None:intergenic
TTAGGCGCAAATATAAATTT+AGG 0.214567 5:+4593832 MsG0580024415.01.T01:CDS
CCTATTTGTGGTCAAGCTTT+TGG 0.243633 5:+4592899 MsG0580024415.01.T01:CDS
TTGACAAGAAAATAGTTTAT+AGG 0.246031 5:-4593205 None:intergenic
GGTTACAATGTTGTTTGTTT+TGG 0.270469 5:+4593963 MsG0580024415.01.T01:CDS
TTTCGCTGATTATTTACATT+TGG 0.272638 5:+4593293 MsG0580024415.01.T01:CDS
ACATGCAACTGTTGCATCTA+TGG 0.280637 5:+4593477 MsG0580024415.01.T01:CDS
TTCGCTGATTATTTACATTT+GGG 0.298088 5:+4593294 MsG0580024415.01.T01:CDS
TTTATATTTGCGCCTAATTT+CGG 0.301593 5:-4593826 None:intergenic
GTTTGAATGTTGGGGATTTA+GGG 0.310488 5:-4592649 None:intergenic
TCTTGTCAATGTTCTTCAAT+TGG 0.313344 5:+4593219 MsG0580024415.01.T01:CDS
ATTGGATTTGCAAACATAAC+AGG 0.318839 5:+4592839 MsG0580024415.01.T01:CDS
GGAATAAGAGGAATCGCTTT+AGG 0.319468 5:+4593241 MsG0580024415.01.T01:CDS
AAAGTAGCACTATATGTTAA+AGG 0.335752 5:-4593163 None:intergenic
CTTGTCAATGTTCTTCAATT+GGG 0.346715 5:+4593220 MsG0580024415.01.T01:CDS
GTTTGGTTGAGTTTCTTTGC+TGG 0.349890 5:+4593886 MsG0580024415.01.T01:CDS
GATTTCAAAGGTTTGTGGTT+TGG 0.352902 5:+4593913 MsG0580024415.01.T01:CDS
TCAATGGTGTTACCGATTAT+AGG 0.360217 5:+4593739 MsG0580024415.01.T01:CDS
GTTCTTCAATTGGGAATAAG+AGG 0.367653 5:+4593229 MsG0580024415.01.T01:CDS
CCAAAAGCTTGACCACAAAT+AGG 0.369074 5:-4592899 None:intergenic
CGTTTGCTATATCTTCTTGT+TGG 0.374870 5:-4593042 None:intergenic
TGCTATGTTTGAAATATGTT+TGG 0.394695 5:-4592711 None:intergenic
ATCATTTCAATGTTGTTCCT+TGG 0.403969 5:+4592776 MsG0580024415.01.T01:CDS
CCTCTGCTTGTTTCAAAGGA+TGG 0.406928 5:+4593353 MsG0580024415.01.T01:CDS
AATGATTGTGCTACAAGATC+AGG 0.410062 5:-4593094 None:intergenic
TTGTGGATATATTATTTGTT+TGG 0.410448 5:-4592574 None:intergenic
AGCAATTCCAAGCTGCATTT+CGG 0.410449 5:+4593393 MsG0580024415.01.T01:CDS
CTCTGGTCTTGCCATGGGAA+TGG 0.412093 5:+4592874 MsG0580024415.01.T01:CDS
AATAATTGCTTTAACATCAA+TGG 0.417803 5:+4593723 MsG0580024415.01.T01:CDS
ATTTCCTCTGCTTGTTTCAA+AGG 0.418055 5:+4593349 MsG0580024415.01.T01:CDS
GAATCGCTTTAGGTTCCGTT+TGG 0.418185 5:+4593251 MsG0580024415.01.T01:CDS
GCTAAAAGATTCAAACTCCT+TGG 0.419870 5:+4592923 MsG0580024415.01.T01:CDS
GGAACGGCGAGGCCGAAATT+AGG 0.422373 5:+4593814 MsG0580024415.01.T01:CDS
ATATGTTAAAGGAAGTGTTA+TGG 0.428714 5:-4593152 None:intergenic
AAACTCAACCAAACTGCAAC+AGG 0.430260 5:-4593877 None:intergenic
AGCTCACATAGTCCTATAAT+CGG 0.440906 5:-4593751 None:intergenic
GCAGGTGGGTCACTTGCCAT+TGG 0.441197 5:+4592821 MsG0580024415.01.T01:CDS
CATTCTCTCTGGTCTTGCCA+TGG 0.443498 5:+4592868 MsG0580024415.01.T01:CDS
GCAAAACTTGCAGCAATAGT+TGG 0.450295 5:+4593607 MsG0580024415.01.T01:CDS
TTTGGTGTTTCAACAAGAGT+TGG 0.450548 5:+4593553 MsG0580024415.01.T01:CDS
GATGTTAAAGCAATTATTTG+TGG 0.453850 5:-4593718 None:intergenic
TGGGAATGCCTGTTGCAGTT+TGG 0.457141 5:+4593869 MsG0580024415.01.T01:CDS
GTTGGTGAGCTTGCTTTAGC+AGG 0.461842 5:+4592803 MsG0580024415.01.T01:CDS
ATCAACGGTGTTAACATGTC+AGG 0.462592 5:-4592626 None:intergenic
AAGGAACAACATTGAAATGA+TGG 0.463159 5:-4592774 None:intergenic
TTTGCTGGATTTGATTTCAA+AGG 0.464102 5:+4593901 MsG0580024415.01.T01:CDS
GATTTGATTTCAAAGGTTTG+TGG 0.465351 5:+4593908 MsG0580024415.01.T01:CDS
ATTCTCTCTGGTCTTGCCAT+GGG 0.465845 5:+4592869 MsG0580024415.01.T01:CDS
GGCTGCTCAAGGTTCATGTA+TGG 0.465974 5:+4593942 MsG0580024415.01.T01:CDS
TTAAAGGAAGTGTTATGGAT+TGG 0.467103 5:-4593147 None:intergenic
CGGTTTGTCTCGAATGGTGG+TGG 0.469476 5:+4593413 MsG0580024415.01.T01:CDS
CATGGCAAGACCAGAGAGAA+TGG 0.471209 5:-4592867 None:intergenic
GCATTTCGGTTTGTCTCGAA+TGG 0.473600 5:+4593407 MsG0580024415.01.T01:CDS
CCAAATGTTTCTAACAGAAA+AGG 0.479393 5:-4593674 None:intergenic
TTCAAACATAGCATTACCTA+TGG 0.482369 5:+4592721 MsG0580024415.01.T01:CDS
AATATGTTTGGCTTCATTGA+GGG 0.484804 5:-4592699 None:intergenic
TTCAAGTGAAGAAGAACAAG+AGG 0.495983 5:+4594041 MsG0580024415.01.T01:CDS
ACAGGTTACTCCATTCTCTC+TGG 0.501242 5:+4592857 MsG0580024415.01.T01:CDS
TTTGGTCTTATGGCTGCTCA+AGG 0.501334 5:+4593931 MsG0580024415.01.T01:CDS
ACACCACAAACAGTTGTTTG+TGG 0.502053 5:-4593784 None:intergenic
GTGAGCTTGCTTTAGCAGGT+GGG 0.506427 5:+4592807 MsG0580024415.01.T01:CDS
ACAAGAGTTGGAAATGAACT+TGG 0.513291 5:+4593565 MsG0580024415.01.T01:CDS
AAATATGTTTGGCTTCATTG+AGG 0.523182 5:-4592700 None:intergenic
ATTATAGGACTATGTGAGCT+TGG 0.533869 5:+4593754 MsG0580024415.01.T01:CDS
AATAAACCTGTTAAAACCAT+AGG 0.534613 5:-4592737 None:intergenic
CTTCGAAGATAGATTCTCAA+AGG 0.539810 5:-4593124 None:intergenic
CATGCAACTGTTGCATCTAT+GGG 0.545154 5:+4593478 MsG0580024415.01.T01:CDS
TGTCCACAAACAACTGTTTG+TGG 0.548402 5:+4593781 MsG0580024415.01.T01:CDS
ACACCAAAACTCAAAGAAGA+AGG 0.550180 5:-4593538 None:intergenic
AGACAAACCGAAATGCAGCT+TGG 0.556967 5:-4593400 None:intergenic
CTGGAACACACAAGAAAACA+TGG 0.559518 5:+4593320 MsG0580024415.01.T01:CDS
TTCTTTGCATTGTTAAACCA+AGG 0.562087 5:-4592940 None:intergenic
GGTGAGCTTGCTTTAGCAGG+TGG 0.562489 5:+4592806 MsG0580024415.01.T01:CDS
CCATCCTTTGAAACAAGCAG+AGG 0.563274 5:-4593353 None:intergenic
GTTATTGCTTTCATACAAAG+TGG 0.571039 5:-4592549 None:intergenic
ATGTTGTTCCTTGGTCGTGT+TGG 0.573577 5:+4592785 MsG0580024415.01.T01:CDS
TGTTATGTTTGCAAATCCAA+TGG 0.576457 5:-4592837 None:intergenic
TTCGAAGATAGATTCTCAAA+GGG 0.581553 5:-4593123 None:intergenic
GAGGTTGAAATTCGTCCAAA+CGG 0.590488 5:-4593266 None:intergenic
GTTGGGGATTTAGGGATCAA+CGG 0.596686 5:-4592641 None:intergenic
TTTCGGTTTGTCTCGAATGG+TGG 0.608886 5:+4593410 MsG0580024415.01.T01:CDS
ATGGGAATGGAACCTATTTG+TGG 0.610601 5:+4592887 MsG0580024415.01.T01:CDS
AAATCAAGCAGAGAGAGCTA+AGG 0.614779 5:+4594002 MsG0580024415.01.T01:CDS
ACAAATAGGTTCCATTCCCA+TGG 0.616628 5:-4592885 None:intergenic
ACACACAAGAAAACATGGAG+TGG 0.626414 5:+4593325 MsG0580024415.01.T01:CDS
ATTATCAACTCAAGTTCATG+TGG 0.629140 5:+4594075 MsG0580024415.01.T01:CDS
CAAGCTCACCAACACGACCA+AGG 0.635498 5:-4592793 None:intergenic
TTCATACAAAGTGGAGACAG+AGG 0.650487 5:-4592540 None:intergenic
ATAGATGCAACAGTTGCATG+TGG 0.665559 5:-4593475 None:intergenic
TGATGATGATAACTTGCACA+TGG 0.742527 5:-4592513 None:intergenic
AAATAATCAGCGAAACAACG+AGG 0.748054 5:-4593285 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATTGCTTTAACATCAA+TGG + Chr5:4593723-4593742 MsG0580024415.01.T01:CDS 20.0%
!! TTGACAAGAAAATAGTTTAT+AGG - Chr5:4593208-4593227 None:intergenic 20.0%
!! TTGTGGATATATTATTTGTT+TGG - Chr5:4592577-4592596 None:intergenic 20.0%
!!! AAGAGATTATTACTTTTATG+TGG + Chr5:4593019-4593038 MsG0580024415.01.T01:CDS 20.0%
!!! CAATTTTCATTTCATTCTTA+TGG + Chr5:4592987-4593006 MsG0580024415.01.T01:CDS 20.0%
! AAAAATGTATATCAATGCAG+TGG - Chr5:4593518-4593537 None:intergenic 25.0%
! AAAGTAGCACTATATGTTAA+AGG - Chr5:4593166-4593185 None:intergenic 25.0%
! AATAAACCTGTTAAAACCAT+AGG - Chr5:4592740-4592759 None:intergenic 25.0%
! GATGTTAAAGCAATTATTTG+TGG - Chr5:4593721-4593740 None:intergenic 25.0%
! TTAGGCGCAAATATAAATTT+AGG + Chr5:4593832-4593851 MsG0580024415.01.T01:CDS 25.0%
! TTTATATTTGCGCCTAATTT+CGG - Chr5:4593829-4593848 None:intergenic 25.0%
!! AAATCATGATTTTGCTTTGT+GGG + Chr5:4593440-4593459 MsG0580024415.01.T01:CDS 25.0%
!! ATTATTTACATTTGGGTTTC+TGG + Chr5:4593301-4593320 MsG0580024415.01.T01:CDS 25.0%
!! CTTTTCTGTTAGAAACATTT+GGG + Chr5:4593675-4593694 MsG0580024415.01.T01:CDS 25.0%
!! TGCTATGTTTGAAATATGTT+TGG - Chr5:4592714-4592733 None:intergenic 25.0%
!! TTCGCTGATTATTTACATTT+GGG + Chr5:4593294-4593313 MsG0580024415.01.T01:CDS 25.0%
!! TTTCGCTGATTATTTACATT+TGG + Chr5:4593293-4593312 MsG0580024415.01.T01:CDS 25.0%
!!! ATATGTTAAAGGAAGTGTTA+TGG - Chr5:4593155-4593174 None:intergenic 25.0%
!!! ATTATTCTGTGTTTTGATTG+TGG - Chr5:4592600-4592619 None:intergenic 25.0%
!!! TCTTTTGTTGTTTGAATGTT+GGG - Chr5:4592661-4592680 None:intergenic 25.0%
!!! TTAGGTTGTTTTTATCTTGT+GGG + Chr5:4593850-4593869 MsG0580024415.01.T01:CDS 25.0%
!!! TTCTTTTGTTGTTTGAATGT+TGG - Chr5:4592662-4592681 None:intergenic 25.0%
!!! TTTAGGTTGTTTTTATCTTG+TGG + Chr5:4593849-4593868 MsG0580024415.01.T01:CDS 25.0%
AAGGAACAACATTGAAATGA+TGG - Chr5:4592777-4592796 None:intergenic 30.0%
ATCATTTCAATGTTGTTCCT+TGG + Chr5:4592776-4592795 MsG0580024415.01.T01:CDS 30.0%
ATTATCAACTCAAGTTCATG+TGG + Chr5:4594075-4594094 MsG0580024415.01.T01:CDS 30.0%
ATTGGATTTGCAAACATAAC+AGG + Chr5:4592839-4592858 MsG0580024415.01.T01:CDS 30.0%
CTTGTCAATGTTCTTCAATT+GGG + Chr5:4593220-4593239 MsG0580024415.01.T01:CDS 30.0%
GTTATTGCTTTCATACAAAG+TGG - Chr5:4592552-4592571 None:intergenic 30.0%
TCTTGTCAATGTTCTTCAAT+TGG + Chr5:4593219-4593238 MsG0580024415.01.T01:CDS 30.0%
TGTTATGTTTGCAAATCCAA+TGG - Chr5:4592840-4592859 None:intergenic 30.0%
TTCAAACATAGCATTACCTA+TGG + Chr5:4592721-4592740 MsG0580024415.01.T01:CDS 30.0%
TTCGAAGATAGATTCTCAAA+GGG - Chr5:4593126-4593145 None:intergenic 30.0%
TTCTTTGCATTGTTAAACCA+AGG - Chr5:4592943-4592962 None:intergenic 30.0%
! AAATATGTTTGGCTTCATTG+AGG - Chr5:4592703-4592722 None:intergenic 30.0%
! AATATGTTTGGCTTCATTGA+GGG - Chr5:4592702-4592721 None:intergenic 30.0%
! CCAAATGTTTCTAACAGAAA+AGG - Chr5:4593677-4593696 None:intergenic 30.0%
! CCTTTTCTGTTAGAAACATT+TGG + Chr5:4593674-4593693 MsG0580024415.01.T01:CDS 30.0%
! GAAATCATGATTTTGCTTTG+TGG + Chr5:4593439-4593458 MsG0580024415.01.T01:CDS 30.0%
! GATTTGATTTCAAAGGTTTG+TGG + Chr5:4593908-4593927 MsG0580024415.01.T01:CDS 30.0%
! TTTGCTGGATTTGATTTCAA+AGG + Chr5:4593901-4593920 MsG0580024415.01.T01:CDS 30.0%
!! GGTTACAATGTTGTTTGTTT+TGG + Chr5:4593963-4593982 MsG0580024415.01.T01:CDS 30.0%
!! TTAAAGGAAGTGTTATGGAT+TGG - Chr5:4593150-4593169 None:intergenic 30.0%
!! TTTCCTTCTTCTTTGAGTTT+TGG + Chr5:4593535-4593554 MsG0580024415.01.T01:CDS 30.0%
!!! ACTCTGTTTCAGTTTTGTTT+TGG + Chr5:4593630-4593649 MsG0580024415.01.T01:CDS 30.0%
!!! CTCTGTTTCAGTTTTGTTTT+GGG + Chr5:4593631-4593650 MsG0580024415.01.T01:CDS 30.0%
!!! CTTTTGTTGTTTGAATGTTG+GGG - Chr5:4592660-4592679 None:intergenic 30.0%
!!! TGTTTTGGTTCGTACAAATT+GGG + Chr5:4593978-4593997 MsG0580024415.01.T01:CDS 30.0%
!!! TTGTTTTGGTTCGTACAAAT+TGG + Chr5:4593977-4593996 MsG0580024415.01.T01:CDS 30.0%
AAATAATCAGCGAAACAACG+AGG - Chr5:4593288-4593307 None:intergenic 35.0%
AATGATTGTGCTACAAGATC+AGG - Chr5:4593097-4593116 None:intergenic 35.0%
ACAAGAGTTGGAAATGAACT+TGG + Chr5:4593565-4593584 MsG0580024415.01.T01:CDS 35.0%
ACACCAAAACTCAAAGAAGA+AGG - Chr5:4593541-4593560 None:intergenic 35.0%
AGAAAAGGCGAAAAACAAAG+CGG - Chr5:4593662-4593681 None:intergenic 35.0%
AGCTCACATAGTCCTATAAT+CGG - Chr5:4593754-4593773 None:intergenic 35.0%
ATTATAGGACTATGTGAGCT+TGG + Chr5:4593754-4593773 MsG0580024415.01.T01:CDS 35.0%
ATTTCCTCTGCTTGTTTCAA+AGG + Chr5:4593349-4593368 MsG0580024415.01.T01:CDS 35.0%
CTTCGAAGATAGATTCTCAA+AGG - Chr5:4593127-4593146 None:intergenic 35.0%
GATTTCAAAGGTTTGTGGTT+TGG + Chr5:4593913-4593932 MsG0580024415.01.T01:CDS 35.0%
GCTAAAAGATTCAAACTCCT+TGG + Chr5:4592923-4592942 MsG0580024415.01.T01:CDS 35.0%
GTTCTTCAATTGGGAATAAG+AGG + Chr5:4593229-4593248 MsG0580024415.01.T01:CDS 35.0%
GTTTGAATGTTGGGGATTTA+GGG - Chr5:4592652-4592671 None:intergenic 35.0%
TGTTTGAATGTTGGGGATTT+AGG - Chr5:4592653-4592672 None:intergenic 35.0%
TTCAAGTGAAGAAGAACAAG+AGG + Chr5:4594041-4594060 MsG0580024415.01.T01:CDS 35.0%
!! AACTGTTTGTGGTGTTTTGA+GGG + Chr5:4593792-4593811 MsG0580024415.01.T01:CDS 35.0%
!! CGTTTGCTATATCTTCTTGT+TGG - Chr5:4593045-4593064 None:intergenic 35.0%
!! TCAATGGTGTTACCGATTAT+AGG + Chr5:4593739-4593758 MsG0580024415.01.T01:CDS 35.0%
!! TTTGGTGTTTCAACAAGAGT+TGG + Chr5:4593553-4593572 MsG0580024415.01.T01:CDS 35.0%
!!! CTGCAAGTTTTGCTTTTTGT+GGG - Chr5:4593600-4593619 None:intergenic 35.0%
!!! GCATTACCTATGGTTTTAAC+AGG + Chr5:4592731-4592750 MsG0580024415.01.T01:CDS 35.0%
AAACTCAACCAAACTGCAAC+AGG - Chr5:4593880-4593899 None:intergenic 40.0%
AAATCAAGCAGAGAGAGCTA+AGG + Chr5:4594002-4594021 MsG0580024415.01.T01:CDS 40.0%
ACAAATAGGTTCCATTCCCA+TGG - Chr5:4592888-4592907 None:intergenic 40.0%
ACACACAAGAAAACATGGAG+TGG + Chr5:4593325-4593344 MsG0580024415.01.T01:CDS 40.0%
ACACCACAAACAGTTGTTTG+TGG - Chr5:4593787-4593806 None:intergenic 40.0%
ACATGCAACTGTTGCATCTA+TGG + Chr5:4593477-4593496 MsG0580024415.01.T01:CDS 40.0%
AGCAATTCCAAGCTGCATTT+CGG + Chr5:4593393-4593412 MsG0580024415.01.T01:CDS 40.0%
ATAGATGCAACAGTTGCATG+TGG - Chr5:4593478-4593497 None:intergenic 40.0%
ATGGGAATGGAACCTATTTG+TGG + Chr5:4592887-4592906 MsG0580024415.01.T01:CDS 40.0%
ATTGAGGGCGAGAGAAAAAT+GGG - Chr5:4592687-4592706 None:intergenic 40.0%
CATGCAACTGTTGCATCTAT+GGG + Chr5:4593478-4593497 MsG0580024415.01.T01:CDS 40.0%
CCAAAAGCTTGACCACAAAT+AGG - Chr5:4592902-4592921 None:intergenic 40.0%
CTGGAACACACAAGAAAACA+TGG + Chr5:4593320-4593339 MsG0580024415.01.T01:CDS 40.0%
GAGGTTGAAATTCGTCCAAA+CGG - Chr5:4593269-4593288 None:intergenic 40.0%
GCAAAACTTGCAGCAATAGT+TGG + Chr5:4593607-4593626 MsG0580024415.01.T01:CDS 40.0%
GGAATAAGAGGAATCGCTTT+AGG + Chr5:4593241-4593260 MsG0580024415.01.T01:CDS 40.0%
GTTTGGTTGAGTTTCTTTGC+TGG + Chr5:4593886-4593905 MsG0580024415.01.T01:CDS 40.0%
TGTCCACAAACAACTGTTTG+TGG + Chr5:4593781-4593800 MsG0580024415.01.T01:CDS 40.0%
TTCATACAAAGTGGAGACAG+AGG - Chr5:4592543-4592562 None:intergenic 40.0%
! ATCAACGGTGTTAACATGTC+AGG - Chr5:4592629-4592648 None:intergenic 40.0%
! CAACTGTTTGTGGTGTTTTG+AGG + Chr5:4593791-4593810 MsG0580024415.01.T01:CDS 40.0%
! CCTATTTGTGGTCAAGCTTT+TGG + Chr5:4592899-4592918 MsG0580024415.01.T01:CDS 40.0%
!! ACTGTTTGTGGTGTTTTGAG+GGG + Chr5:4593793-4593812 MsG0580024415.01.T01:CDS 40.0%
!! AGGTTTGTGGTTTGGTCTTA+TGG + Chr5:4593921-4593940 MsG0580024415.01.T01:CDS 40.0%
!!! CTGTGTTTTGATTGTGGTTG+TGG - Chr5:4592594-4592613 None:intergenic 40.0%
!!! GCTGCAAGTTTTGCTTTTTG+TGG - Chr5:4593601-4593620 None:intergenic 40.0%
ACAGGTTACTCCATTCTCTC+TGG + Chr5:4592857-4592876 MsG0580024415.01.T01:CDS 45.0%
AGACAAACCGAAATGCAGCT+TGG - Chr5:4593403-4593422 None:intergenic 45.0%
ATGTTGTTCCTTGGTCGTGT+TGG + Chr5:4592785-4592804 MsG0580024415.01.T01:CDS 45.0%
CATTGAGGGCGAGAGAAAAA+TGG - Chr5:4592688-4592707 None:intergenic 45.0%
CCTCTGCTTGTTTCAAAGGA+TGG + Chr5:4593353-4593372 MsG0580024415.01.T01:CDS 45.0%
GTTGGGGATTTAGGGATCAA+CGG - Chr5:4592644-4592663 None:intergenic 45.0%
! GAATCGCTTTAGGTTCCGTT+TGG + Chr5:4593251-4593270 MsG0580024415.01.T01:CDS 45.0%
! GCATTTCGGTTTGTCTCGAA+TGG + Chr5:4593407-4593426 MsG0580024415.01.T01:CDS 45.0%
! TTTCGGTTTGTCTCGAATGG+TGG + Chr5:4593410-4593429 MsG0580024415.01.T01:CDS 45.0%
!! ATTCTCTCTGGTCTTGCCAT+GGG + Chr5:4592869-4592888 MsG0580024415.01.T01:CDS 45.0%
!! CCATCCTTTGAAACAAGCAG+AGG - Chr5:4593356-4593375 None:intergenic 45.0%
!! TTTGGTCTTATGGCTGCTCA+AGG + Chr5:4593931-4593950 MsG0580024415.01.T01:CDS 45.0%
!!! TTGTGGTGTTTTGAGGGGAA+CGG + Chr5:4593798-4593817 MsG0580024415.01.T01:CDS 45.0%
CATGGCAAGACCAGAGAGAA+TGG - Chr5:4592870-4592889 None:intergenic 50.0%
GGCTGCTCAAGGTTCATGTA+TGG + Chr5:4593942-4593961 MsG0580024415.01.T01:CDS 50.0%
TGGGAATGCCTGTTGCAGTT+TGG + Chr5:4593869-4593888 MsG0580024415.01.T01:CDS 50.0%
! GTGAGCTTGCTTTAGCAGGT+GGG + Chr5:4592807-4592826 MsG0580024415.01.T01:CDS 50.0%
! GTTGGTGAGCTTGCTTTAGC+AGG + Chr5:4592803-4592822 MsG0580024415.01.T01:CDS 50.0%
!! CATTCTCTCTGGTCTTGCCA+TGG + Chr5:4592868-4592887 MsG0580024415.01.T01:CDS 50.0%
CAAGCTCACCAACACGACCA+AGG - Chr5:4592796-4592815 None:intergenic 55.0%
CGGTTTGTCTCGAATGGTGG+TGG + Chr5:4593413-4593432 MsG0580024415.01.T01:CDS 55.0%
! GGTGAGCTTGCTTTAGCAGG+TGG + Chr5:4592806-4592825 MsG0580024415.01.T01:CDS 55.0%
!! CTCTGGTCTTGCCATGGGAA+TGG + Chr5:4592874-4592893 MsG0580024415.01.T01:CDS 55.0%
GCAGGTGGGTCACTTGCCAT+TGG + Chr5:4592821-4592840 MsG0580024415.01.T01:CDS 60.0%
GGAACGGCGAGGCCGAAATT+AGG + Chr5:4593814-4593833 MsG0580024415.01.T01:CDS 60.0%
!!! GTGTTTTGAGGGGAACGGCG+AGG + Chr5:4593803-4593822 MsG0580024415.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 4592515 4594128 4592515 ID=MsG0580024415.01;Name=MsG0580024415.01
Chr5 mRNA 4592515 4594128 4592515 ID=MsG0580024415.01.T01;Parent=MsG0580024415.01;Name=MsG0580024415.01.T01;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|537
Chr5 exon 4592515 4594128 4592515 ID=MsG0580024415.01.T01:exon:2296;Parent=MsG0580024415.01.T01
Chr5 CDS 4592515 4594128 4592515 ID=MsG0580024415.01.T01:cds;Parent=MsG0580024415.01.T01
Gene Sequence

>MsG0580024415.01.T01

ATGTGCAAGTTATCATCATCATCAACCTCTGTCTCCACTTTGTATGAAAGCAATAACAACCAAACAAATAATATATCCACAACCACAATCAAAACACAGAATAATATCAAACCTGACATGTTAACACCGTTGATCCCTAAATCCCCAACATTCAAACAACAAAAGAAAACCCATTTTTCTCTCGCCCTCAATGAAGCCAAACATATTTCAAACATAGCATTACCTATGGTTTTAACAGGTTTATTACTTTATTCTCGTTCCATCATTTCAATGTTGTTCCTTGGTCGTGTTGGTGAGCTTGCTTTAGCAGGTGGGTCACTTGCCATTGGATTTGCAAACATAACAGGTTACTCCATTCTCTCTGGTCTTGCCATGGGAATGGAACCTATTTGTGGTCAAGCTTTTGGTGCTAAAAGATTCAAACTCCTTGGTTTAACAATGCAAAGAACAGTGATTCTTCTTCTTGTAACTTCAATTTTCATTTCATTCTTATGGCTTAACATGAAGAGATTATTACTTTTATGTGGCCAACAAGAAGATATAGCAAACGTTGCACAATCTTACATTCTTTATTCTCTTCCTGATCTTGTAGCACAATCATTGCTACACCCTTTGAGAATCTATCTTCGAAGCCAATCCATAACACTTCCTTTAACATATAGTGCTACTTTGTCTATTCTTCTTCACATTCCTATAAACTATTTTCTTGTCAATGTTCTTCAATTGGGAATAAGAGGAATCGCTTTAGGTTCCGTTTGGACGAATTTCAACCTCGTTGTTTCGCTGATTATTTACATTTGGGTTTCTGGAACACACAAGAAAACATGGAGTGGAATTTCCTCTGCTTGTTTCAAAGGATGGAAATCGCTTTTGAATTTAGCAATTCCAAGCTGCATTTCGGTTTGTCTCGAATGGTGGTGGTACGAAATCATGATTTTGCTTTGTGGGTTATTGCTTAATCCACATGCAACTGTTGCATCTATGGGTGTGTTGATTCAAACCACTGCATTGATATACATTTTTCCTTCTTCTTTGAGTTTTGGTGTTTCAACAAGAGTTGGAAATGAACTTGGTGCTGAAAACCCACAAAAAGCAAAACTTGCAGCAATAGTTGGACTCTGTTTCAGTTTTGTTTTGGGTTTCTCCGCTTTGTTTTTCGCCTTTTCTGTTAGAAACATTTGGGCTACTATGTTCACAAGTGATCCACAAATAATTGCTTTAACATCAATGGTGTTACCGATTATAGGACTATGTGAGCTTGGAAATTGTCCACAAACAACTGTTTGTGGTGTTTTGAGGGGAACGGCGAGGCCGAAATTAGGCGCAAATATAAATTTAGGTTGTTTTTATCTTGTGGGAATGCCTGTTGCAGTTTGGTTGAGTTTCTTTGCTGGATTTGATTTCAAAGGTTTGTGGTTTGGTCTTATGGCTGCTCAAGGTTCATGTATGGTTACAATGTTGTTTGTTTTGGTTCGTACAAATTGGGAAAATCAAGCAGAGAGAGCTAAGGAGTTAACATCATCAGATTCAAGTGAAGAAGAACAAGAGGAAGAGAAAGTGATTATCAACTCAAGTTCATGTGGCACAAAAGAGTGCTCTGATTCATTAGTTTGA
Protein sequence

>MsG0580024415.01.T01

MCKLSSSSTSVSTLYESNNNQTNNISTTTIKTQNNIKPDMLTPLIPKSPTFKQQKKTHFSLALNEAKHISNIALPMVLTGLLLYSRSIISMLFLGRVGELALAGGSLAIGFANITGYSILSGLAMGMEPICGQAFGAKRFKLLGLTMQRTVILLLVTSIFISFLWLNMKRLLLLCGQQEDIANVAQSYILYSLPDLVAQSLLHPLRIYLRSQSITLPLTYSATLSILLHIPINYFLVNVLQLGIRGIALGSVWTNFNLVVSLIIYIWVSGTHKKTWSGISSACFKGWKSLLNLAIPSCISVCLEWWWYEIMILLCGLLLNPHATVASMGVLIQTTALIYIFPSSLSFGVSTRVGNELGAENPQKAKLAAIVGLCFSFVLGFSALFFAFSVRNIWATMFTSDPQIIALTSMVLPIIGLCELGNCPQTTVCGVLRGTARPKLGANINLGCFYLVGMPVAVWLSFFAGFDFKGLWFGLMAAQGSCMVTMLFVLVRTNWENQAERAKELTSSDSSEEEQEEEKVIINSSSCGTKECSDSLV*