AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280008228.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008228.01.T01 MTR_2g036740 95.703 256 8 1 1 256 1 253 0.0 506
MsG0280008228.01.T01 MTR_4g127680 39.604 202 78 5 57 256 4 163 6.52e-31 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008228.01.T01 AT5G57340 34.420 276 117 10 1 255 1 233 3.14e-21 89.7
MsG0280008228.01.T01 AT5G57340 34.420 276 117 10 1 255 19 251 7.92e-21 89.0

Find 60 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAATAGTATTAAACTTTA+TGG 0.093335 2:+27484165 MsG0280008228.01.T01:five_prime_UTR
ATAATTCCTCTTTCAAAATT+TGG 0.105021 2:-27486449 None:intergenic
CTTGGCAGTTCATATTCTTT+TGG 0.175183 2:-27484002 None:intergenic
TACATTTGATCAAGATAAAA+AGG 0.235098 2:+27486749 MsG0280008228.01.T01:CDS
TTTACTTTATTCCAATAAAA+TGG 0.251455 2:+27486929 MsG0280008228.01.T01:three_prime_UTR
GGACCACTCAATTTATTATA+TGG 0.252972 2:+27486872 MsG0280008228.01.T01:three_prime_UTR
TGTTGTTGAGGTAATAATTT+AGG 0.265185 2:-27483917 None:intergenic
GAAAGAAGTGGAAGTTGTTC+AGG 0.278248 2:+27486480 MsG0280008228.01.T01:CDS
TATCTGCATTGTCTTTCTAA+TGG 0.285749 2:-27486014 None:intergenic
AGTAACAAACATTTCAATAT+TGG 0.294801 2:+27485935 MsG0280008228.01.T01:CDS
TGAGTATGCTAGAGGATTTA+TGG 0.299297 2:-27485840 None:intergenic
GTGGATGATTAATATCTATA+TGG 0.332345 2:-27484122 None:intergenic
ATACCTATGCTACCATTCAT+AGG 0.347754 2:-27486369 None:intergenic
GAAAGAGGAATTATACATAA+AGG 0.351691 2:+27486458 MsG0280008228.01.T01:CDS
TCATCACAATCACTTGTTGC+TGG 0.354721 2:-27486546 None:intergenic
GGTCCATGTTTAGTGTAAAT+AGG 0.365817 2:-27486854 None:intergenic
CACTTGTGTTATATTAGTAT+TGG 0.386558 2:+27484142 MsG0280008228.01.T01:five_prime_UTR
ATATGAATTTGAAGTATGTA+AGG 0.393550 2:+27487052 MsG0280008228.01.T01:three_prime_UTR
TCTTCAAATTCTTCAGTGCT+TGG 0.405096 2:+27484049 MsG0280008228.01.T01:five_prime_UTR
GTGGATTTGACTTTATGTAT+AGG 0.411271 2:+27486579 MsG0280008228.01.T01:CDS
GAGCTGCTCATTGGTGTAGT+GGG 0.415795 2:-27486720 None:intergenic
GTGTGGTTGGTGTTGATGCC+TGG 0.418366 2:+27485882 MsG0280008228.01.T01:CDS
GGTAATAATTTAGGTTTGAC+AGG 0.427224 2:-27483908 None:intergenic
CTGTCAAGTATCCTATCCAT+TGG 0.443227 2:-27485977 None:intergenic
GAAGGAGGCAAGGCAAAAGC+TGG 0.453555 2:-27483877 None:intergenic
GTTGTTGAACAAAATAAGGT+TGG 0.453849 2:-27486395 None:intergenic
TACCTATGCTACCATTCATA+GGG 0.460339 2:-27486368 None:intergenic
AAGTGATTGTGATGAAGATA+TGG 0.468753 2:+27486554 MsG0280008228.01.T01:CDS
AAGTCTTATATGCTTCCAAA+TGG 0.472702 2:+27486630 MsG0280008228.01.T01:CDS
GCACTGAAGAATTTGAAGAT+AGG 0.487276 2:-27484045 None:intergenic
AGAGCTGCTCATTGGTGTAG+TGG 0.503631 2:-27486721 None:intergenic
TCCAAATGGAATGAAAACTA+AGG 0.522393 2:+27486644 MsG0280008228.01.T01:CDS
GTAACACTAGAGCTGCTCAT+TGG 0.525161 2:-27486729 None:intergenic
ATTCCTATTTACACTAAACA+TGG 0.531539 2:+27486851 MsG0280008228.01.T01:three_prime_UTR
TAATAACTTGCAAAATCCAA+TGG 0.546440 2:+27485961 MsG0280008228.01.T01:CDS
TGACTAAATGAAATTTGTGT+TGG 0.550065 2:-27486423 None:intergenic
ACTTGCAAAATCCAATGGAT+AGG 0.552356 2:+27485966 MsG0280008228.01.T01:CDS
GGGCCACATTGGCTTTCTCA+AGG 0.553022 2:+27486771 MsG0280008228.01.T01:CDS
CAATACTAATATAACACAAG+TGG 0.553056 2:-27484141 None:intergenic
GGCTGTGAAGGAAGGAGGCA+AGG 0.558019 2:-27483887 None:intergenic
GTTTGACAGGCTGTGAAGGA+AGG 0.558750 2:-27483895 None:intergenic
TTGTGATGAAGATATGGAAG+TGG 0.570304 2:+27486560 MsG0280008228.01.T01:CDS
TTTGTTCTGATAATGCTGCC+AGG 0.573507 2:-27485900 None:intergenic
CTGGAATAATGATGTTGTTG+AGG 0.583711 2:-27483929 None:intergenic
AAACATCAACAAAGCAACCT+AGG 0.585277 2:-27483975 None:intergenic
TGCAACAACTTCAGTGTGGT+TGG 0.585657 2:+27485869 MsG0280008228.01.T01:CDS
TTAGGTTTGACAGGCTGTGA+AGG 0.588272 2:-27483899 None:intergenic
AGCTGCTCATTGGTGTAGTG+GGG 0.600539 2:-27486719 None:intergenic
AAAGACAATGCAGATACAAG+AGG 0.602336 2:+27486021 MsG0280008228.01.T01:CDS
TTATACATAAAGGAAAGAAG+TGG 0.603251 2:+27486468 MsG0280008228.01.T01:CDS
CAATCTACTGAGTATGCTAG+AGG 0.610316 2:-27485848 None:intergenic
TGACAGGCTGTGAAGGAAGG+AGG 0.624890 2:-27483892 None:intergenic
GAACCATATAATAAATTGAG+TGG 0.626635 2:-27486875 None:intergenic
ATAGACAGCATGAAGACACT+TGG 0.628877 2:-27484020 None:intergenic
ACCCCTATGAATGGTAGCAT+AGG 0.633561 2:+27486366 MsG0280008228.01.T01:CDS
TGGCAGCATTATCAGAACAA+AGG 0.642212 2:+27485902 MsG0280008228.01.T01:CDS
AAACCTTGAGAAAGCCAATG+TGG 0.666219 2:-27486774 None:intergenic
ACCTATGCTACCATTCATAG+GGG 0.677238 2:-27486367 None:intergenic
GATACTTGACAGAAACAACA+TGG 0.687441 2:+27485988 MsG0280008228.01.T01:CDS
AGATTGCAACAACTTCAGTG+TGG 0.741905 2:+27485865 MsG0280008228.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATAAAAATAAAAGGA+CGG - Chr2:27484081-27484100 None:intergenic 10.0%
!! AATTGAAAAAAATAAAATGA+TGG - Chr2:27485412-27485431 None:intergenic 10.0%
!! TAAAAAAAAAATTTCTTACA+TGG + Chr2:27484429-27484448 MsG0280008228.01.T01:intron 10.0%
!! TTGAAGAATAATAATAAAAT+AGG - Chr2:27484990-27485009 None:intergenic 10.0%
!! TTGGAAAAAATAAAAATAAA+AGG - Chr2:27484085-27484104 None:intergenic 10.0%
!! TTTAATAGTATTAAACTTTA+TGG + Chr2:27484165-27484184 MsG0280008228.01.T01:five_prime_UTR 10.0%
!!! CAATTTTTTTATATAAAAAC+TGG - Chr2:27483951-27483970 None:intergenic 10.0%
!!! TTGTAAGTTTTTTATTTTTT+CGG + Chr2:27485745-27485764 MsG0280008228.01.T01:intron 10.0%
!! ATTAGTATTTGAAAATTTGT+AGG + Chr2:27486260-27486279 MsG0280008228.01.T01:intron 15.0%
!! TTCTATTAATATTTAGTGTT+TGG + Chr2:27485148-27485167 MsG0280008228.01.T01:intron 15.0%
!! TTTACTTTATTCCAATAAAA+TGG + Chr2:27486929-27486948 MsG0280008228.01.T01:three_prime_UTR 15.0%
!!! ATAATTTTGTGAAGAAATAA+TGG - Chr2:27485193-27485212 None:intergenic 15.0%
!!! ATATAAGTTATCCATTTTAT+TGG - Chr2:27486943-27486962 None:intergenic 15.0%
!! ACATTTGATCAAGATAAAAA+GGG + Chr2:27486750-27486769 MsG0280008228.01.T01:CDS 20.0%
!! AGTAACAAACATTTCAATAT+TGG + Chr2:27485935-27485954 MsG0280008228.01.T01:CDS 20.0%
!! ATAAAGTAAAAAACTCATCT+AGG - Chr2:27486919-27486938 None:intergenic 20.0%
!! ATAATTCCTCTTTCAAAATT+TGG - Chr2:27486452-27486471 None:intergenic 20.0%
!! ATTTGAAAATTTGTAGGTAA+AGG + Chr2:27486266-27486285 MsG0280008228.01.T01:intron 20.0%
!! GTCAAAATATTCTTAGAATA+AGG + Chr2:27485124-27485143 MsG0280008228.01.T01:intron 20.0%
!! TAAGTGTATATATATGTGAA+GGG + Chr2:27484572-27484591 MsG0280008228.01.T01:intron 20.0%
!! TACATTTGATCAAGATAAAA+AGG + Chr2:27486749-27486768 MsG0280008228.01.T01:CDS 20.0%
!! TATAAAAAAATTGTTCACCT+AGG + Chr2:27483958-27483977 MsG0280008228.01.T01:five_prime_UTR 20.0%
!! TATGTTATTAAGTTAGTTAG+AGG - Chr2:27485498-27485517 None:intergenic 20.0%
!! TTAAGTGTATATATATGTGA+AGG + Chr2:27484571-27484590 MsG0280008228.01.T01:intron 20.0%
!!! AAGATAATATTTTCAGATCA+TGG + Chr2:27485081-27485100 MsG0280008228.01.T01:intron 20.0%
!!! ATATGAATTTGAAGTATGTA+AGG + Chr2:27487052-27487071 MsG0280008228.01.T01:three_prime_UTR 20.0%
!!! TTTTGTTGTTGAACAAAATA+AGG - Chr2:27486402-27486421 None:intergenic 20.0%
! ATGAAGTACATATACATACA+CGG - Chr2:27484546-27484565 None:intergenic 25.0%
! ATTCCTATTTACACTAAACA+TGG + Chr2:27486851-27486870 MsG0280008228.01.T01:three_prime_UTR 25.0%
! CAATACTAATATAACACAAG+TGG - Chr2:27484144-27484163 None:intergenic 25.0%
! CACTTGTGTTATATTAGTAT+TGG + Chr2:27484142-27484161 MsG0280008228.01.T01:five_prime_UTR 25.0%
! CATTTGATCAAGATAAAAAG+GGG + Chr2:27486751-27486770 MsG0280008228.01.T01:CDS 25.0%
! GAAAGAGGAATTATACATAA+AGG + Chr2:27486458-27486477 MsG0280008228.01.T01:CDS 25.0%
! GAACCATATAATAAATTGAG+TGG - Chr2:27486878-27486897 None:intergenic 25.0%
! GCATCAAATAAAAACATAGT+TGG - Chr2:27486194-27486213 None:intergenic 25.0%
! GTGGATGATTAATATCTATA+TGG - Chr2:27484125-27484144 None:intergenic 25.0%
! TACTAAACCAAAAATAGAAG+AGG - Chr2:27484917-27484936 None:intergenic 25.0%
! TGACTAAATGAAATTTGTGT+TGG - Chr2:27486426-27486445 None:intergenic 25.0%
! TGTTGTTGAGGTAATAATTT+AGG - Chr2:27483920-27483939 None:intergenic 25.0%
! TTATACATAAAGGAAAGAAG+TGG + Chr2:27486468-27486487 MsG0280008228.01.T01:CDS 25.0%
! TTGAATCTATGCATACAAAA+AGG + Chr2:27485041-27485060 MsG0280008228.01.T01:intron 25.0%
!! AAGGCAAGCAAAATAATTTT+TGG + Chr2:27486343-27486362 MsG0280008228.01.T01:CDS 25.0%
!! TAATAACTTGCAAAATCCAA+TGG + Chr2:27485961-27485980 MsG0280008228.01.T01:CDS 25.0%
!! TCTTTTCAATCAGATAGAAT+TGG + Chr2:27486684-27486703 MsG0280008228.01.T01:CDS 25.0%
!!! TAAGTATTGATTTGCATTTC+AGG + Chr2:27485794-27485813 MsG0280008228.01.T01:intron 25.0%
AAGTGATTGTGATGAAGATA+TGG + Chr2:27486554-27486573 MsG0280008228.01.T01:CDS 30.0%
ACCTTATGGTACAAATTGAA+TGG - Chr2:27486109-27486128 None:intergenic 30.0%
ATGGATGAACACAAAAATGA+AGG + Chr2:27486324-27486343 MsG0280008228.01.T01:CDS 30.0%
ATGGTTTGAATTTCTGTTGT+TGG + Chr2:27485100-27485119 MsG0280008228.01.T01:intron 30.0%
CAATGTGCAAAAAATGCTAA+TGG + Chr2:27486305-27486324 MsG0280008228.01.T01:CDS 30.0%
GAGTAAATTTGTCAAAACAG+AGG - Chr2:27484946-27484965 None:intergenic 30.0%
GGACCACTCAATTTATTATA+TGG + Chr2:27486872-27486891 MsG0280008228.01.T01:three_prime_UTR 30.0%
GTTGTTGAACAAAATAAGGT+TGG - Chr2:27486398-27486417 None:intergenic 30.0%
TAAACTAGACATTGACACTT+TGG - Chr2:27485686-27485705 None:intergenic 30.0%
TAAGCTTAAAGTATGAAGCA+CGG + Chr2:27484467-27484486 MsG0280008228.01.T01:intron 30.0%
TAGAGACTAACAAGTCTAAT+AGG - Chr2:27485275-27485294 None:intergenic 30.0%
TATCTGCATTGTCTTTCTAA+TGG - Chr2:27486017-27486036 None:intergenic 30.0%
TCATTCATCATCAAAGTTTC+TGG + Chr2:27484835-27484854 MsG0280008228.01.T01:intron 30.0%
TCCAAATGGAATGAAAACTA+AGG + Chr2:27486644-27486663 MsG0280008228.01.T01:CDS 30.0%
TCCATTCAATTTGTACCATA+AGG + Chr2:27486105-27486124 MsG0280008228.01.T01:intron 30.0%
TCTTAAGCACAAACAGAAAA+GGG + Chr2:27485226-27485245 MsG0280008228.01.T01:intron 30.0%
TCTTCACTCAAATAACATGA+AGG + Chr2:27484324-27484343 MsG0280008228.01.T01:intron 30.0%
TGGAATATAACTACGTATGT+TGG - Chr2:27485392-27485411 None:intergenic 30.0%
! AAGTCTTATATGCTTCCAAA+TGG + Chr2:27486630-27486649 MsG0280008228.01.T01:CDS 30.0%
! ACTAGTTCCTCTTCTATTTT+TGG + Chr2:27484907-27484926 MsG0280008228.01.T01:intron 30.0%
! AGTTGGTACTAAATGACAAT+AGG - Chr2:27486177-27486196 None:intergenic 30.0%
! ATATGGTTTTCCTTGAATCA+TGG - Chr2:27484108-27484127 None:intergenic 30.0%
! GGTAATAATTTAGGTTTGAC+AGG - Chr2:27483911-27483930 None:intergenic 30.0%
! GTGGATTTGACTTTATGTAT+AGG + Chr2:27486579-27486598 MsG0280008228.01.T01:CDS 30.0%
! TTGGCAGTTCATATTCTTTT+GGG - Chr2:27484004-27484023 None:intergenic 30.0%
!! ATTGATTTGCATTTCAGGAA+TGG + Chr2:27485799-27485818 MsG0280008228.01.T01:intron 30.0%
!! ATTTTGCTACTTCTTACAAC+AGG + Chr2:27484247-27484266 MsG0280008228.01.T01:five_prime_UTR 30.0%
!! TCATGTCCAAATTTTGAAAG+AGG + Chr2:27486443-27486462 MsG0280008228.01.T01:CDS 30.0%
!! TCCTTAGTTTTCATTCCATT+TGG - Chr2:27486648-27486667 None:intergenic 30.0%
!! TGCATAGTTTCTAGTAATAG+TGG + Chr2:27484749-27484768 MsG0280008228.01.T01:intron 30.0%
!! TTGATTTGCATTTCAGGAAT+GGG + Chr2:27485800-27485819 MsG0280008228.01.T01:intron 30.0%
!!! TTTTTTCCAACCATGATTCA+AGG + Chr2:27484095-27484114 MsG0280008228.01.T01:five_prime_UTR 30.0%
AAACATCAACAAAGCAACCT+AGG - Chr2:27483978-27483997 None:intergenic 35.0%
AAAGACAATGCAGATACAAG+AGG + Chr2:27486021-27486040 MsG0280008228.01.T01:CDS 35.0%
AACATATGTCGATGTCATGT+CGG - Chr2:27485649-27485668 None:intergenic 35.0%
ACTTGCAAAATCCAATGGAT+AGG + Chr2:27485966-27485985 MsG0280008228.01.T01:CDS 35.0%
AGAGGATATCTTCAAAAACC+AGG + Chr2:27486039-27486058 MsG0280008228.01.T01:CDS 35.0%
ATACCTATGCTACCATTCAT+AGG - Chr2:27486372-27486391 None:intergenic 35.0%
CTCTTAAGCACAAACAGAAA+AGG + Chr2:27485225-27485244 MsG0280008228.01.T01:intron 35.0%
CTGGAATAATGATGTTGTTG+AGG - Chr2:27483932-27483951 None:intergenic 35.0%
CTTCAATCAGATTGAAGTGT+AGG - Chr2:27485607-27485626 None:intergenic 35.0%
GATACTTGACAGAAACAACA+TGG + Chr2:27485988-27486007 MsG0280008228.01.T01:CDS 35.0%
GATCACAAGACTTTAAGTGT+AGG - Chr2:27485583-27485602 None:intergenic 35.0%
GATGAATGAACATTATGTGC+AGG - Chr2:27484824-27484843 None:intergenic 35.0%
GCACTGAAGAATTTGAAGAT+AGG - Chr2:27484048-27484067 None:intergenic 35.0%
GGTCCATGTTTAGTGTAAAT+AGG - Chr2:27486857-27486876 None:intergenic 35.0%
TACCTATGCTACCATTCATA+GGG - Chr2:27486371-27486390 None:intergenic 35.0%
TACTACATTGACATGATACC+TGG - Chr2:27486060-27486079 None:intergenic 35.0%
TATCATGCTATGCAACCTTA+TGG - Chr2:27486123-27486142 None:intergenic 35.0%
TCTTCAAATTCTTCAGTGCT+TGG + Chr2:27484049-27484068 MsG0280008228.01.T01:five_prime_UTR 35.0%
TGAGTATGCTAGAGGATTTA+TGG - Chr2:27485843-27485862 None:intergenic 35.0%
TTGTGATGAAGATATGGAAG+TGG + Chr2:27486560-27486579 MsG0280008228.01.T01:CDS 35.0%
! CTTGGCAGTTCATATTCTTT+TGG - Chr2:27484005-27484024 None:intergenic 35.0%
!! AATTTTTGGACCCCTATGAA+TGG + Chr2:27486357-27486376 MsG0280008228.01.T01:CDS 35.0%
AAACCTTGAGAAAGCCAATG+TGG - Chr2:27486777-27486796 None:intergenic 40.0%
AAGATAAAAAGGGGCCACAT+TGG + Chr2:27486760-27486779 MsG0280008228.01.T01:CDS 40.0%
AATCTGATTGAAGTCGTGTC+TGG + Chr2:27485614-27485633 MsG0280008228.01.T01:intron 40.0%
ACCTATGCTACCATTCATAG+GGG - Chr2:27486370-27486389 None:intergenic 40.0%
AGATTGCAACAACTTCAGTG+TGG + Chr2:27485865-27485884 MsG0280008228.01.T01:CDS 40.0%
AGTGTAGGAGATTGAAGTGT+AGG - Chr2:27485568-27485587 None:intergenic 40.0%
ATAGACAGCATGAAGACACT+TGG - Chr2:27484023-27484042 None:intergenic 40.0%
CAATCTACTGAGTATGCTAG+AGG - Chr2:27485851-27485870 None:intergenic 40.0%
CTGTCAAGTATCCTATCCAT+TGG - Chr2:27485980-27485999 None:intergenic 40.0%
CTTCAGATAGAAGACGTGTA+TGG + Chr2:27485340-27485359 MsG0280008228.01.T01:intron 40.0%
GAAAGAAGTGGAAGTTGTTC+AGG + Chr2:27486480-27486499 MsG0280008228.01.T01:CDS 40.0%
GTATGGTGACTAACACGTAT+TGG + Chr2:27485357-27485376 MsG0280008228.01.T01:intron 40.0%
GTGGATGAAGACTACTGTAA+TGG + Chr2:27484768-27484787 MsG0280008228.01.T01:intron 40.0%
TGGCAGCATTATCAGAACAA+AGG + Chr2:27485902-27485921 MsG0280008228.01.T01:CDS 40.0%
TTGAGTAAGAGAGAGAGAGA+GGG - Chr2:27483815-27483834 None:intergenic 40.0%
TTTGAGTAAGAGAGAGAGAG+AGG - Chr2:27483816-27483835 None:intergenic 40.0%
! AAGTTAGTTAGAGGAAGCAC+CGG - Chr2:27485489-27485508 None:intergenic 40.0%
! ACGTCTTCTATCTGAAGTGT+CGG - Chr2:27485337-27485356 None:intergenic 40.0%
! GGTTTTCCTTGAATCATGGT+TGG - Chr2:27484104-27484123 None:intergenic 40.0%
! TCATCACAATCACTTGTTGC+TGG - Chr2:27486549-27486568 None:intergenic 40.0%
! TGACTAACACGTATTGGTGT+TGG + Chr2:27485363-27485382 MsG0280008228.01.T01:intron 40.0%
! TTTGTTCTGATAATGCTGCC+AGG - Chr2:27485903-27485922 None:intergenic 40.0%
ACCCCTATGAATGGTAGCAT+AGG + Chr2:27486366-27486385 MsG0280008228.01.T01:CDS 45.0%
GTAACACTAGAGCTGCTCAT+TGG - Chr2:27486732-27486751 None:intergenic 45.0%
TCCAATATCCGTGTCTTTGC+CGG + Chr2:27485467-27485486 MsG0280008228.01.T01:intron 45.0%
TGCAACAACTTCAGTGTGGT+TGG + Chr2:27485869-27485888 MsG0280008228.01.T01:CDS 45.0%
TTAGGTTTGACAGGCTGTGA+AGG - Chr2:27483902-27483921 None:intergenic 45.0%
! CGTGTTTCTGTCACAAGACT+AGG - Chr2:27484795-27484814 None:intergenic 45.0%
!!! TTATTTTTTTCAATTATTAT+TGG + Chr2:27485417-27485436 MsG0280008228.01.T01:intron 5.0%
ACCGGCAAAGACACGGATAT+TGG - Chr2:27485471-27485490 None:intergenic 50.0%
ACTGAGACACGCCCAATCTA+AGG - Chr2:27484498-27484517 None:intergenic 50.0%
AGAGCTGCTCATTGGTGTAG+TGG - Chr2:27486724-27486743 None:intergenic 50.0%
AGCACGGACACTCCTTAGAT+TGG + Chr2:27484483-27484502 MsG0280008228.01.T01:intron 50.0%
AGCTGCTCATTGGTGTAGTG+GGG - Chr2:27486722-27486741 None:intergenic 50.0%
GAGCTGCTCATTGGTGTAGT+GGG - Chr2:27486723-27486742 None:intergenic 50.0%
GCACGGACACTCCTTAGATT+GGG + Chr2:27484484-27484503 MsG0280008228.01.T01:intron 50.0%
GTTTGACAGGCTGTGAAGGA+AGG - Chr2:27483898-27483917 None:intergenic 50.0%
GAAGGAGGCAAGGCAAAAGC+TGG - Chr2:27483880-27483899 None:intergenic 55.0%
GATTGGGCGTGTCTCAGTGT+CGG + Chr2:27484500-27484519 MsG0280008228.01.T01:intron 55.0%
TGACAGGCTGTGAAGGAAGG+AGG - Chr2:27483895-27483914 None:intergenic 55.0%
TGTCGGATACGTGTCGTGTC+TGG + Chr2:27484517-27484536 MsG0280008228.01.T01:intron 55.0%
!! AGGAAGCACCGGCAAAGACA+CGG - Chr2:27485478-27485497 None:intergenic 55.0%
!! GGGCCACATTGGCTTTCTCA+AGG + Chr2:27486771-27486790 MsG0280008228.01.T01:CDS 55.0%
!! GTGTGGTTGGTGTTGATGCC+TGG + Chr2:27485882-27485901 MsG0280008228.01.T01:CDS 55.0%
GGCTGTGAAGGAAGGAGGCA+AGG - Chr2:27483890-27483909 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 27483761 27487084 27483761 ID=MsG0280008228.01;Name=MsG0280008228.01
Chr2 mRNA 27483761 27487084 27483761 ID=MsG0280008228.01.T01;Parent=MsG0280008228.01;Name=MsG0280008228.01.T01;_AED=0.28;_eAED=0.28;_QI=510|1|1|1|0.5|0.33|3|275|256
Chr2 exon 27483761 27484268 27483761 ID=MsG0280008228.01.T01:exon:18804;Parent=MsG0280008228.01.T01
Chr2 exon 27485816 27486060 27485816 ID=MsG0280008228.01.T01:exon:18805;Parent=MsG0280008228.01.T01
Chr2 exon 27486282 27487084 27486282 ID=MsG0280008228.01.T01:exon:18806;Parent=MsG0280008228.01.T01
Chr2 five_prime_UTR 27483761 27484268 27483761 ID=MsG0280008228.01.T01:five_prime_utr;Parent=MsG0280008228.01.T01
Chr2 five_prime_UTR 27485816 27485817 27485816 ID=MsG0280008228.01.T01:five_prime_utr;Parent=MsG0280008228.01.T01
Chr2 CDS 27485818 27486060 27485818 ID=MsG0280008228.01.T01:cds;Parent=MsG0280008228.01.T01
Chr2 CDS 27486282 27486809 27486282 ID=MsG0280008228.01.T01:cds;Parent=MsG0280008228.01.T01
Chr2 three_prime_UTR 27486810 27487084 27486810 ID=MsG0280008228.01.T01:three_prime_utr;Parent=MsG0280008228.01.T01
Gene Sequence

>MsG0280008228.01.T01

ATGGGAACAAAAATTGAATATTCCATAAATCCTCTAGCATACTCAGTAGATTGCAACAACTTCAGTGTGGTTGGTGTTGATGCCTGGCAGCATTATCAGAACAAAGGATTGAAAGTGAGTAACAAACATTTCAATATTGGAGTTAATAACTTGCAAAATCCAATGGATAGGATACTTGACAGAAACAACATGGAATCCATTAGAAAGACAATGCAGATACAAGAGGATATCTTCAAAAACCAGGTAAAGGAATTACATAGAGTATACAATGTGCAAAAAATGCTAATGGATGAACACAAAAATGAAGGCAAGCAAAATAATTTTTGGACCCCTATGAATGGTAGCATAGGTATAAACCAACCTTATTTTGTTCAACAACAAAAACCAACACAAATTTCATTTAGTCATGTCCAAATTTTGAAAGAGGAATTATACATAAAGGAAAGAAGTGGAAGTTGTTCAGGAGAAATAATCAAGAAAAGACAAAAAGATTTTGATCTTGAAAAGCCAGCAACAAGTGATTGTGATGAAGATATGGAAGTGGATTTGACTTTATGTATAGGAAGCAATGAAAGTAAGAATAAGAAGAAAAAGTCTTATATGCTTCCAAATGGAATGAAAACTAAGGAGTTAAATTCTTATCTCTCTTTTCAATCAGATAGAATTGGAGATTATAGTGACCCCACTACACCAATGAGCAGCTCTAGTGTTACATTTGATCAAGATAAAAAGGGGCCACATTGGCTTTCTCAAGGTTTAAAGCTTAAATAG
Protein sequence

>MsG0280008228.01.T01

MGTKIEYSINPLAYSVDCNNFSVVGVDAWQHYQNKGLKVSNKHFNIGVNNLQNPMDRILDRNNMESIRKTMQIQEDIFKNQVKELHRVYNVQKMLMDEHKNEGKQNNFWTPMNGSIGINQPYFVQQQKPTQISFSHVQILKEELYIKERSGSCSGEIIKKRQKDFDLEKPATSDCDEDMEVDLTLCIGSNESKNKKKKSYMLPNGMKTKELNSYLSFQSDRIGDYSDPTTPMSSSSVTFDQDKKGPHWLSQGLKLK*