AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280009451.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009451.01.T01 MTR_2g063510 78.723 282 7 3 1 230 110 390 3.08e-154 434
MsG0280009451.01.T01 MTR_8g447000 67.399 273 34 4 9 229 114 383 1.64e-123 355
MsG0280009451.01.T01 MTR_2g063510 70.297 202 8 2 1 150 110 311 1.15e-88 265
MsG0280009451.01.T01 MTR_8g447000 59.043 188 23 4 9 144 114 299 6.25e-62 196
MsG0280009451.01.T01 MTR_4g007070 38.421 190 63 3 90 226 136 324 2.38e-44 152
MsG0280009451.01.T01 MTR_3g111690 37.387 222 83 4 59 226 97 316 6.48e-44 150
MsG0280009451.01.T01 MTR_0009s0230 36.788 193 68 2 89 228 110 301 9.46e-43 147
MsG0280009451.01.T01 MTR_0009s0230 36.269 193 68 3 89 228 110 300 7.12e-40 139
MsG0280009451.01.T01 MTR_4g130880 45.865 133 70 2 96 228 207 337 1.33e-35 129
MsG0280009451.01.T01 MTR_8g066040 41.611 149 70 3 96 228 175 322 3.10e-34 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009451.01.T01 AT3G12710 56.835 278 52 5 5 228 46 309 1.20e-99 292
MsG0280009451.01.T01 AT5G44680 49.801 251 65 3 31 230 113 353 4.81e-82 249
MsG0280009451.01.T01 AT1G80850 41.711 187 55 3 91 224 134 319 1.42e-45 154
MsG0280009451.01.T01 AT1G75090 39.153 189 61 2 91 226 119 306 2.17e-45 154
MsG0280009451.01.T01 AT5G57970 40.758 211 65 5 73 224 130 339 8.44e-45 153
MsG0280009451.01.T01 AT5G57970 40.758 211 65 5 73 224 130 339 8.44e-45 153
MsG0280009451.01.T01 AT1G13635 37.073 205 75 2 77 228 101 304 4.18e-44 150
MsG0280009451.01.T01 AT1G13635 37.073 205 75 2 77 228 101 304 4.18e-44 150
MsG0280009451.01.T01 AT1G15970 38.743 191 63 3 91 228 145 334 3.03e-43 149
MsG0280009451.01.T01 AT1G15970 38.743 191 63 3 91 228 145 334 3.03e-43 149

Find 71 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCACTTGCAATGCACATTAT+TGG 0.261232 2:-52299893 MsG0280009451.01.T01:CDS
GTCATTTGACAAATACATTT+GGG 0.265730 2:-52300106 MsG0280009451.01.T01:CDS
ATCTCAAATCAATACAAATT+TGG 0.303182 2:-52300063 MsG0280009451.01.T01:CDS
GGCAATGGAGAAAGTGATTT+TGG 0.310365 2:+52302581 None:intergenic
TTTCCTCTCTTTATAGCTAA+TGG 0.311064 2:+52302483 None:intergenic
CTAGTAGGTGACTTTGGTTT+TGG 0.311738 2:+52302513 None:intergenic
AAAATGAAGATTGCTCATTA+TGG 0.312266 2:-52302243 MsG0280009451.01.T01:CDS
AGTTTCAAAGAAGGGTCTTT+TGG 0.315301 2:-52302369 MsG0280009451.01.T01:CDS
GAGTTTCTTCTTTCAAGATT+TGG 0.322751 2:+52302620 None:intergenic
CATAATGAAAACTCCAACTT+TGG 0.328226 2:-52302409 MsG0280009451.01.T01:exon
GGGTCAATTAATCTCTTATA+TGG 0.340630 2:+52299867 None:intergenic
GGTCATTTGACAAATACATT+TGG 0.343231 2:-52300107 MsG0280009451.01.T01:CDS
TGATTAAGTGGTCATTTGTT+AGG 0.343650 2:+52299926 None:intergenic
CTAACAGCAGCTATGCTTCC+TGG 0.345680 2:+52302300 None:intergenic
TAGAAAATATTAACCTGAAT+TGG 0.350713 2:+52302113 None:intergenic
GAAGCATAGCTGCTGTTAGA+AGG 0.354512 2:-52302296 MsG0280009451.01.T01:CDS
TACAGTTACTTCTTTAGCCT+TGG 0.358427 2:+52302720 None:intergenic
TGATGTCTTAGTTCAGAATA+TGG 0.368289 2:-52301144 MsG0280009451.01.T01:intron
AAGAGAGGAAATGACAATAA+TGG 0.373376 2:-52302471 MsG0280009451.01.T01:five_prime_UTR
GAAGAAGTAGTAGTCTTGTT+TGG 0.385879 2:+52302554 None:intergenic
TGCTTATCATGATGAAGAAT+GGG 0.396248 2:-52301802 MsG0280009451.01.T01:CDS
TTACAGGTTAAGAAAGGCTT+TGG 0.404283 2:-52300128 MsG0280009451.01.T01:intron
AGAGCACTTGAAGGATCAAT+AGG 0.410745 2:+52302186 None:intergenic
AATTTGTATTGATTTGAGAT+TGG 0.419291 2:+52300066 None:intergenic
TTGCATGTTGGTTGAAGTAC+TGG 0.429795 2:+52302650 None:intergenic
CTAGTACTAGTAGGTGACTT+TGG 0.431001 2:+52302507 None:intergenic
CATTCATTTATGCAGGCAGC+TGG 0.444232 2:-52299949 MsG0280009451.01.T01:CDS
GAGGAAATGTTGTGAGGGAT+TGG 0.451044 2:+52302845 None:intergenic
TTGCTTATCATGATGAAGAA+TGG 0.451345 2:-52301803 MsG0280009451.01.T01:CDS
TCATGATAAGCAATATAGAT+AGG 0.456509 2:+52301813 None:intergenic
TTTGAATCAAGAGCACTTGA+AGG 0.459539 2:+52302177 None:intergenic
CCACATGTTGCTAGAATCAA+TGG 0.460004 2:-52302676 MsG0280009451.01.T01:five_prime_UTR
AGCTTCATCACAGCCAATTC+AGG 0.467872 2:-52302126 MsG0280009451.01.T01:intron
GCTTACTTGTCATCATGAAC+TGG 0.468369 2:+52301774 None:intergenic
ATTGAAATAAGCAAAGTTAG+AGG 0.477981 2:-52301120 MsG0280009451.01.T01:CDS
AAGAGAGGGTTTAGATATGT+TGG 0.481696 2:-52299985 MsG0280009451.01.T01:CDS
GCTAATGGTCTAGTACTAGT+AGG 0.483572 2:+52302498 None:intergenic
GTCTTGTTTGGAAGTGGCAA+TGG 0.488397 2:+52302566 None:intergenic
TTCTTTCAAGATTTGGAACA+TGG 0.493504 2:+52302627 None:intergenic
GTAGTAGTCTTGTTTGGAAG+TGG 0.500686 2:+52302560 None:intergenic
AGTGGTTCATTCATTTATGC+AGG 0.501189 2:-52299956 MsG0280009451.01.T01:CDS
AGCAAAGACATGATTAAGAG+AGG 0.510022 2:-52300000 MsG0280009451.01.T01:CDS
GAAATGTTGTGAGGGATTGG+AGG 0.534495 2:+52302848 None:intergenic
AATGAAAAGAGAGGAAAATG+AGG 0.542743 2:+52302826 None:intergenic
AAGTCAAAGAGTTTCAAAGA+AGG 0.556152 2:-52302378 MsG0280009451.01.T01:CDS
AAGCATAGCTGCTGTTAGAA+GGG 0.560846 2:-52302295 MsG0280009451.01.T01:CDS
AAATGTTGTGAGGGATTGGA+GGG 0.563520 2:+52302849 None:intergenic
GCAAAGACATGATTAAGAGA+GGG 0.567917 2:-52299999 MsG0280009451.01.T01:CDS
AGTCAAAGAGTTTCAAAGAA+GGG 0.568686 2:-52302377 MsG0280009451.01.T01:CDS
TTCAAAGACTACTAATCAAG+AGG 0.580713 2:-52302160 MsG0280009451.01.T01:CDS
GCCTGTGGCAAGTGATTAAG+TGG 0.581468 2:+52299914 None:intergenic
AAAATGAGGAAATGTTGTGA+GGG 0.583527 2:+52302840 None:intergenic
TGCTGTTAGAAGGGAACAAG+TGG 0.590285 2:-52302286 MsG0280009451.01.T01:CDS
TCTAGCAACATGTGGTGTTG+TGG 0.592032 2:+52302684 None:intergenic
TAAGATTGCAATGAAAAGAG+AGG 0.595315 2:+52302817 None:intergenic
CATTTGACAAATACATTTGG+GGG 0.595980 2:-52300104 MsG0280009451.01.T01:CDS
TCATTTGACAAATACATTTG+GGG 0.597984 2:-52300105 MsG0280009451.01.T01:CDS
ATAAATGAATGAACCACTGT+TGG 0.599012 2:+52299961 None:intergenic
GAAAATGAGGAAATGTTGTG+AGG 0.603695 2:+52302839 None:intergenic
AAGGCTAAAGAAGTAACTGT+AGG 0.608755 2:-52302718 MsG0280009451.01.T01:five_prime_UTR
ACCACTTAATCACTTGCCAC+AGG 0.609569 2:-52299915 MsG0280009451.01.T01:CDS
TCTTTGATCACTGATTCACC+AGG 0.613842 2:-52302318 MsG0280009451.01.T01:CDS
ACAAAACATGTGTAGCTCCA+AGG 0.617528 2:-52302737 MsG0280009451.01.T01:five_prime_UTR
TAGATATGTTGGTCCAACAG+TGG 0.633858 2:-52299974 MsG0280009451.01.T01:CDS
CCATTGATTCTAGCAACATG+TGG 0.642807 2:+52302676 None:intergenic
TTTGGAACATGGTTGCATGT+TGG 0.648256 2:+52302638 None:intergenic
TGAAAACTCCAACTTTGGAG+AGG 0.649673 2:-52302404 MsG0280009451.01.T01:CDS
AGACCATTAGCTATAAAGAG+AGG 0.661843 2:-52302486 MsG0280009451.01.T01:five_prime_UTR
ATGTGCATTGCAAGTGCCTG+TGG 0.666497 2:+52299899 None:intergenic
GCTTATCATGATGAAGAATG+GGG 0.675468 2:-52301801 MsG0280009451.01.T01:CDS
TGGCACTTCAACAAGCACAA+AGG 0.702109 2:-52302266 MsG0280009451.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTAAAATAATAAGAAG+TGG + Chr2:52301793-52301812 None:intergenic 10.0%
!!! TAATTTTAAATTTATGTACA+GGG - Chr2:52301508-52301527 MsG0280009451.01.T01:intron 10.0%
!! ATGATTTATATACAAAGTAA+AGG + Chr2:52302260-52302279 None:intergenic 15.0%
!! TTAAATGTAATTACATGTAT+TGG - Chr2:52301103-52301122 MsG0280009451.01.T01:CDS 15.0%
!!! AAACATTTTTAATGACTAAA+TGG + Chr2:52300661-52300680 None:intergenic 15.0%
!!! CTAATTTTAAATTTATGTAC+AGG - Chr2:52301507-52301526 MsG0280009451.01.T01:intron 15.0%
!!! TCTTAAAAAATTGTTTTGAA+TGG - Chr2:52302539-52302558 MsG0280009451.01.T01:five_prime_UTR 15.0%
!!! TTTTTTTTACCTGTAAAATT+TGG + Chr2:52301653-52301672 None:intergenic 15.0%
!! ATCTCAAATCAATACAAATT+TGG - Chr2:52302666-52302685 MsG0280009451.01.T01:five_prime_UTR 20.0%
!! ATGTATTTACAATTGATCAA+AGG - Chr2:52302336-52302355 MsG0280009451.01.T01:CDS 20.0%
!! ATTTGAACTTCTAATTTAAG+TGG - Chr2:52301354-52301373 MsG0280009451.01.T01:intron 20.0%
!! TAGAAAATATTAACCTGAAT+TGG + Chr2:52300619-52300638 None:intergenic 20.0%
!!! AATTTGTATTGATTTGAGAT+TGG + Chr2:52302666-52302685 None:intergenic 20.0%
!!! CTATTTTCTCAAAATATTAC+TGG + Chr2:52301078-52301097 None:intergenic 20.0%
! AAAATGAAGATTGCTCATTA+TGG - Chr2:52300486-52300505 MsG0280009451.01.T01:intron 25.0%
! AACAGAAACTTATAAGCTAT+TGG + Chr2:52301857-52301876 None:intergenic 25.0%
! AACTTATCTACATAAGTTCT+AGG + Chr2:52302143-52302162 None:intergenic 25.0%
! ATTGAAATAAGCAAAGTTAG+AGG - Chr2:52301609-52301628 MsG0280009451.01.T01:intron 25.0%
! ATTGCACTTAGTATCAAAAA+AGG + Chr2:52300841-52300860 None:intergenic 25.0%
! GCTTATTTACAATTGATCAA+AGG - Chr2:52302358-52302377 MsG0280009451.01.T01:CDS 25.0%
! GTCATTTGACAAATACATTT+GGG - Chr2:52302623-52302642 MsG0280009451.01.T01:five_prime_UTR 25.0%
! TAATGACTAAATGGTTACAA+TGG + Chr2:52300652-52300671 None:intergenic 25.0%
! TACAAAGTAAAGGATAAACA+AGG + Chr2:52302250-52302269 None:intergenic 25.0%
! TCATGATAAGCAATATAGAT+AGG + Chr2:52300919-52300938 None:intergenic 25.0%
! TCATTTGACAAATACATTTG+GGG - Chr2:52302624-52302643 MsG0280009451.01.T01:five_prime_UTR 25.0%
!! GGAGTTTTCATTATGTTTTT+TGG + Chr2:52300315-52300334 None:intergenic 25.0%
!! TAATGCTAACCAAATTTTAC+AGG - Chr2:52301641-52301660 MsG0280009451.01.T01:intron 25.0%
!! TAGTTTATATGTGTTTCTGA+AGG + Chr2:52301248-52301267 None:intergenic 25.0%
!!! AAAGTGTATGAGATTTTTTG+AGG + Chr2:52299949-52299968 None:intergenic 25.0%
!!! ACATTAATTTTTTCGCTCTA+GGG - Chr2:52301479-52301498 MsG0280009451.01.T01:intron 25.0%
!!! TACATTAATTTTTTCGCTCT+AGG - Chr2:52301478-52301497 MsG0280009451.01.T01:intron 25.0%
!!! TTGCAGTTAATTTTAACACA+AGG - Chr2:52301976-52301995 MsG0280009451.01.T01:intron 25.0%
AAAATGAGGAAATGTTGTGA+GGG + Chr2:52299892-52299911 None:intergenic 30.0%
AAGAGAGGAAATGACAATAA+TGG - Chr2:52300258-52300277 MsG0280009451.01.T01:intron 30.0%
AAGTCAAAGAGTTTCAAAGA+AGG - Chr2:52300351-52300370 MsG0280009451.01.T01:intron 30.0%
AATGAAAAGAGAGGAAAATG+AGG + Chr2:52299906-52299925 None:intergenic 30.0%
AGAATTTGATGCAGAAATTG+TGG - Chr2:52301541-52301560 MsG0280009451.01.T01:intron 30.0%
AGGCTTATATACAAAGACAA+AGG - Chr2:52302378-52302397 MsG0280009451.01.T01:CDS 30.0%
AGTCAAAGAGTTTCAAAGAA+GGG - Chr2:52300352-52300371 MsG0280009451.01.T01:intron 30.0%
AGTTCAAATAACATCCTGAA+TGG + Chr2:52301343-52301362 None:intergenic 30.0%
ATAAATGAATGAACCACTGT+TGG + Chr2:52302771-52302790 None:intergenic 30.0%
ATGCCTTCAATCTCAAATAT+TGG - Chr2:52301169-52301188 MsG0280009451.01.T01:intron 30.0%
ATTCAGCTGTTTATCAAGAT+AGG + Chr2:52302004-52302023 None:intergenic 30.0%
ATTGATCAATCAACTCATGT+GGG + Chr2:52301886-52301905 None:intergenic 30.0%
CACATGTGTGACTTTATATT+AGG + Chr2:52300700-52300719 None:intergenic 30.0%
CACTAATCAACGTATTTAGT+CGG - Chr2:52302182-52302201 MsG0280009451.01.T01:CDS 30.0%
CATAATGAAAACTCCAACTT+TGG - Chr2:52300320-52300339 MsG0280009451.01.T01:intron 30.0%
CATTTGACAAATACATTTGG+GGG - Chr2:52302625-52302644 MsG0280009451.01.T01:five_prime_UTR 30.0%
CATTTGCTTATCAGTCAAAT+TGG + Chr2:52301567-52301586 None:intergenic 30.0%
GAGTTTCTTCTTTCAAGATT+TGG + Chr2:52300112-52300131 None:intergenic 30.0%
GGTCATTTGACAAATACATT+TGG - Chr2:52302622-52302641 MsG0280009451.01.T01:five_prime_UTR 30.0%
TAAGATTGCAATGAAAAGAG+AGG + Chr2:52299915-52299934 None:intergenic 30.0%
TATCTTGATAAACAGCTGAA+TGG - Chr2:52302003-52302022 MsG0280009451.01.T01:intron 30.0%
TATGTTACACACAACTATGA+AGG - Chr2:52300992-52301011 MsG0280009451.01.T01:intron 30.0%
TATTGAGATGTGTCAAACAT+CGG - Chr2:52301144-52301163 MsG0280009451.01.T01:intron 30.0%
TGATGTCTTAGTTCAGAATA+TGG - Chr2:52301585-52301604 MsG0280009451.01.T01:intron 30.0%
TGATTAAGTGGTCATTTGTT+AGG + Chr2:52302806-52302825 None:intergenic 30.0%
TGCTTATCATGATGAAGAAT+GGG - Chr2:52300927-52300946 MsG0280009451.01.T01:intron 30.0%
TTCAAAGACTACTAATCAAG+AGG - Chr2:52300569-52300588 MsG0280009451.01.T01:intron 30.0%
TTGCTTATCATGATGAAGAA+TGG - Chr2:52300926-52300945 MsG0280009451.01.T01:intron 30.0%
TTTCCTCTCTTTATAGCTAA+TGG + Chr2:52300249-52300268 None:intergenic 30.0%
! ACACCAATATTTGAGATTGA+AGG + Chr2:52301175-52301194 None:intergenic 30.0%
! TTCTTTCAAGATTTGGAACA+TGG + Chr2:52300105-52300124 None:intergenic 30.0%
!! AATATGTGACAGCATTGATA+AGG + Chr2:52301922-52301941 None:intergenic 30.0%
!! CTTTGAAATTATGCCATTAC+AGG - Chr2:52302585-52302604 MsG0280009451.01.T01:five_prime_UTR 30.0%
AAGAGAGGGTTTAGATATGT+TGG - Chr2:52302744-52302763 MsG0280009451.01.T01:five_prime_UTR 35.0%
AAGCCTTTCTTAACCTGTAA+TGG + Chr2:52302601-52302620 None:intergenic 35.0%
AAGGCTAAAGAAGTAACTGT+AGG - Chr2:52300011-52300030 MsG0280009451.01.T01:CDS 35.0%
AATTCACTCGTTGCCAAAAA+AGG - Chr2:52301814-52301833 MsG0280009451.01.T01:CDS 35.0%
AATTTAAGTGGTGCTCAAGT+TGG - Chr2:52301366-52301385 MsG0280009451.01.T01:intron 35.0%
AGACCATTAGCTATAAAGAG+AGG - Chr2:52300243-52300262 MsG0280009451.01.T01:intron 35.0%
AGCAAAGACATGATTAAGAG+AGG - Chr2:52302729-52302748 MsG0280009451.01.T01:five_prime_UTR 35.0%
AGTGGTTCATTCATTTATGC+AGG - Chr2:52302773-52302792 MsG0280009451.01.T01:five_prime_UTR 35.0%
ATGCCATTACAGGTTAAGAA+AGG - Chr2:52302595-52302614 MsG0280009451.01.T01:five_prime_UTR 35.0%
CATTGATCAATCAACTCATG+TGG + Chr2:52301887-52301906 None:intergenic 35.0%
GAAAATGAGGAAATGTTGTG+AGG + Chr2:52299893-52299912 None:intergenic 35.0%
GCAAAGACATGATTAAGAGA+GGG - Chr2:52302730-52302749 MsG0280009451.01.T01:five_prime_UTR 35.0%
GCTTATCATGATGAAGAATG+GGG - Chr2:52300928-52300947 MsG0280009451.01.T01:intron 35.0%
GTAACATAGAGAGAGAAGTA+GGG + Chr2:52300980-52300999 None:intergenic 35.0%
TACAGTTACTTCTTTAGCCT+TGG + Chr2:52300012-52300031 None:intergenic 35.0%
TATATGATGTGTGTCCATTC+AGG - Chr2:52301326-52301345 MsG0280009451.01.T01:intron 35.0%
TGTAACATAGAGAGAGAAGT+AGG + Chr2:52300981-52301000 None:intergenic 35.0%
TTACAGGTTAAGAAAGGCTT+TGG - Chr2:52302601-52302620 MsG0280009451.01.T01:five_prime_UTR 35.0%
TTGATCAATCAACTCATGTG+GGG + Chr2:52301885-52301904 None:intergenic 35.0%
! AGTTTCAAAGAAGGGTCTTT+TGG - Chr2:52300360-52300379 MsG0280009451.01.T01:intron 35.0%
!! AATTAGAGCATTGAGAAGTC+AGG + Chr2:52302434-52302453 None:intergenic 35.0%
!! GAAGAAGTAGTAGTCTTGTT+TGG + Chr2:52300178-52300197 None:intergenic 35.0%
!! TAGTGAGTGTTGATAGCTTA+TGG + Chr2:52302167-52302186 None:intergenic 35.0%
!! TATTGGTGTCATCTTTGTGT+TGG - Chr2:52301120-52301139 MsG0280009451.01.T01:CDS 35.0%
!! TCTGATTTTGATGTCTTCAC+TGG + Chr2:52302702-52302721 None:intergenic 35.0%
!! TTTGAATCAAGAGCACTTGA+AGG + Chr2:52300555-52300574 None:intergenic 35.0%
!!! TGACTTTGGTTTTGGAGAAT+TGG + Chr2:52300211-52300230 None:intergenic 35.0%
AAACACCTGCGTTATTCGTA+AGG + Chr2:52302494-52302513 None:intergenic 40.0%
AAGCATAGCTGCTGTTAGAA+GGG - Chr2:52300434-52300453 MsG0280009451.01.T01:intron 40.0%
ACAAAACATGTGTAGCTCCA+AGG - Chr2:52299992-52300011 MsG0280009451.01.T01:CDS 40.0%
CAGAATCAAACCTGAAGTCT+AGG + Chr2:52301422-52301441 None:intergenic 40.0%
CCACATGTTGCTAGAATCAA+TGG - Chr2:52300053-52300072 MsG0280009451.01.T01:CDS 40.0%
CTAGTACTAGTAGGTGACTT+TGG + Chr2:52300225-52300244 None:intergenic 40.0%
GCACTTGCAATGCACATTAT+TGG - Chr2:52302836-52302855 MsG0280009451.01.T01:five_prime_UTR 40.0%
GCATTGAGAAGTCAGGATAA+TGG + Chr2:52302427-52302446 None:intergenic 40.0%
GCTAATGGTCTAGTACTAGT+AGG + Chr2:52300234-52300253 None:intergenic 40.0%
GCTTACTTGTCATCATGAAC+TGG + Chr2:52300958-52300977 None:intergenic 40.0%
TAGATATGTTGGTCCAACAG+TGG - Chr2:52302755-52302774 MsG0280009451.01.T01:five_prime_UTR 40.0%
! AGAGCACTTGAAGGATCAAT+AGG + Chr2:52300546-52300565 None:intergenic 40.0%
! GGCAATGGAGAAAGTGATTT+TGG + Chr2:52300151-52300170 None:intergenic 40.0%
! TGAAAACTCCAACTTTGGAG+AGG - Chr2:52300325-52300344 MsG0280009451.01.T01:intron 40.0%
! TTGCATGTTGGTTGAAGTAC+TGG + Chr2:52300082-52300101 None:intergenic 40.0%
! TTTGGAACATGGTTGCATGT+TGG + Chr2:52300094-52300113 None:intergenic 40.0%
!! ATTGGTGTCGAATACTGACA+CGG - Chr2:52301187-52301206 MsG0280009451.01.T01:intron 40.0%
!! CCATTGATTCTAGCAACATG+TGG + Chr2:52300056-52300075 None:intergenic 40.0%
!! CTAGTAGGTGACTTTGGTTT+TGG + Chr2:52300219-52300238 None:intergenic 40.0%
!! GACTTTTTCCTCTCCAAAGT+TGG + Chr2:52300336-52300355 None:intergenic 40.0%
!! GTAGTAGTCTTGTTTGGAAG+TGG + Chr2:52300172-52300191 None:intergenic 40.0%
!! TCTTTGATCACTGATTCACC+AGG - Chr2:52300411-52300430 MsG0280009451.01.T01:intron 40.0%
!!! AACTTGTCTGCAGCCTTTTT+TGG + Chr2:52301830-52301849 None:intergenic 40.0%
!!! CTTTGGTTTTGGAGAATTGG+TGG + Chr2:52300208-52300227 None:intergenic 40.0%
ACCACTTAATCACTTGCCAC+AGG - Chr2:52302814-52302833 MsG0280009451.01.T01:five_prime_UTR 45.0%
AGCTTCATCACAGCCAATTC+AGG - Chr2:52300603-52300622 MsG0280009451.01.T01:intron 45.0%
AGGACACAACTATGAAGCAC+CGG - Chr2:52301012-52301031 MsG0280009451.01.T01:intron 45.0%
CATTCATTTATGCAGGCAGC+TGG - Chr2:52302780-52302799 MsG0280009451.01.T01:five_prime_UTR 45.0%
GAAGCATAGCTGCTGTTAGA+AGG - Chr2:52300433-52300452 MsG0280009451.01.T01:intron 45.0%
GAAGTCTAGGCGTTTCTTCA+AGG + Chr2:52301409-52301428 None:intergenic 45.0%
GAGGAAATGTTGTGAGGGAT+TGG + Chr2:52299887-52299906 None:intergenic 45.0%
GCTATCCTTACGAATAACGC+AGG - Chr2:52302486-52302505 MsG0280009451.01.T01:five_prime_UTR 45.0%
TAGCCTGTATCAACACTCAC+TGG + Chr2:52302470-52302489 None:intergenic 45.0%
TCTGATGTATGTGCATGTGC+CGG + Chr2:52301034-52301053 None:intergenic 45.0%
TGAAGAAACGCCTAGACTTC+AGG - Chr2:52301409-52301428 MsG0280009451.01.T01:intron 45.0%
TGCTGTTAGAAGGGAACAAG+TGG - Chr2:52300443-52300462 MsG0280009451.01.T01:intron 45.0%
TGGCACTTCAACAAGCACAA+AGG - Chr2:52300463-52300482 MsG0280009451.01.T01:intron 45.0%
! TCTAGCAACATGTGGTGTTG+TGG + Chr2:52300048-52300067 None:intergenic 45.0%
!! CGGTTAAAGCATCGATGAGT+CGG - Chr2:52302202-52302221 MsG0280009451.01.T01:CDS 45.0%
!! GTCTTGTTTGGAAGTGGCAA+TGG + Chr2:52300166-52300185 None:intergenic 45.0%
!! GTGCTCAAGTTGGATCTGAT+TGG - Chr2:52301376-52301395 MsG0280009451.01.T01:intron 45.0%
!! TAGCCAGTGAGTGTTGATAC+AGG - Chr2:52302464-52302483 MsG0280009451.01.T01:five_prime_UTR 45.0%
ATGTGCATTGCAAGTGCCTG+TGG + Chr2:52302833-52302852 None:intergenic 50.0%
CTAACAGCAGCTATGCTTCC+TGG + Chr2:52300432-52300451 None:intergenic 50.0%
GCCTGTGGCAAGTGATTAAG+TGG + Chr2:52302818-52302837 None:intergenic 50.0%
GTCTAGGCGTTTCTTCAAGG+TGG + Chr2:52301406-52301425 None:intergenic 50.0%
TAGGCGTTTCTTCAAGGTGG+AGG + Chr2:52301403-52301422 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr2 gene 52299882 52302869 52299882 ID=MsG0280009451.01;Name=MsG0280009451.01
Chr2 mRNA 52299882 52302869 52299882 ID=MsG0280009451.01.T01;Parent=MsG0280009451.01;Name=MsG0280009451.01.T01;_AED=0.37;_eAED=0.37;_QI=442|0.33|0.5|1|0.66|0.5|4|0|230
Chr2 exon 52299882 52300138 52299882 ID=MsG0280009451.01.T01:exon:6792;Parent=MsG0280009451.01.T01
Chr2 exon 52301079 52301155 52301079 ID=MsG0280009451.01.T01:exon:6791;Parent=MsG0280009451.01.T01
Chr2 exon 52301780 52301837 52301780 ID=MsG0280009451.01.T01:exon:6790;Parent=MsG0280009451.01.T01
Chr2 exon 52302127 52302869 52302127 ID=MsG0280009451.01.T01:exon:6789;Parent=MsG0280009451.01.T01
Chr2 five_prime_UTR 52302428 52302869 52302428 ID=MsG0280009451.01.T01:five_prime_utr;Parent=MsG0280009451.01.T01
Chr2 CDS 52302127 52302427 52302127 ID=MsG0280009451.01.T01:cds;Parent=MsG0280009451.01.T01
Chr2 CDS 52301780 52301837 52301780 ID=MsG0280009451.01.T01:cds;Parent=MsG0280009451.01.T01
Chr2 CDS 52301079 52301155 52301079 ID=MsG0280009451.01.T01:cds;Parent=MsG0280009451.01.T01
Chr2 CDS 52299882 52300138 52299882 ID=MsG0280009451.01.T01:cds;Parent=MsG0280009451.01.T01
Gene Sequence

>MsG0280009451.01.T01

ATGAAAACTCCAACTTTGGAGAGGAAAAAGTCAAAGAGTTTCAAAGAAGGGTCTTTTGGTATTGAAGCTTCATTGAGTTATTCTTCTTCTTTGATCACTGATTCACCAGGAAGCATAGCTGCTGTTAGAAGGGAACAAGTGGCACTTCAACAAGCACAAAGGAAAATGAAGATTGCTCATTATGGAAGATCAAAGTCTGCTAAATTTGAAAGAGTTTTTCCTATTGATCCTTCAAGTGCTCTTGATTCAAAGACTACTAATCAAGAGGAGAAGAGATGCAGCTTCATCACAGCCAATTCAGATCCTATCTATATTGCTTATCATGATGAAGAATGGGGAGTTCCAGTTCATGATGACAATTCAGAATATGGCATTGAAATAAGCAAAGTTAGAGGAGTTGTTGATAATGCTAACCAAATTTTACAGGTAAAAAAAAAAAGGCTTTGGTCATTTGACAAATACATTTGGGGGTTTGTGAATCATAAACCAATCTCAAATCAATACAAATTTGGTCACAAGATTCCAGTGAAGACATCAAAATCAGAGAGCATAAGCAAAGACATGATTAAGAGAGGGTTTAGATATGTTGGTCCAACAGTGGTTCATTCATTTATGCAGGCAGCTGGCCTAACAAATGACCACTTAATCACTTGCCACAGGCACTTGCAATGCACATTATTGGCAGCCATATAA
Protein sequence

>MsG0280009451.01.T01

MKTPTLERKKSKSFKEGSFGIEASLSYSSSLITDSPGSIAAVRREQVALQQAQRKMKIAHYGRSKSAKFERVFPIDPSSALDSKTTNQEEKRCSFITANSDPIYIAYHDEEWGVPVHDDNSEYGIEISKVRGVVDNANQILQVKKKRLWSFDKYIWGFVNHKPISNQYKFGHKIPVKTSKSESISKDMIKRGFRYVGPTVVHSFMQAAGLTNDHLITCHRHLQCTLLAAI*