AlfalfaGEDB | MsG0880046237.01

M. sativa cultivar ZhongmuNo.1 / MsG0880046237.01

KEGG: K01940
GO: GO:0004055 GO:0005524 GO:0006526
PFAM: PF00764

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046237.01.T01	GAU44984.1	98.78	82	1	0	6	87	172	253	4.54E-51	177

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046237.01.T01	Q9SZX3	72.414	87	23	1	1	87	196	281	1.22E-37	134

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046237.01.T01	A0A2Z6NKP2	98.780	82	1	0	6	87	172	253	2.17e-51	177

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048687.01	MsG0880046237.01	0.812843	3.456816e-51	2.221343e-48
MsG0180000364.01	MsG0880046237.01	0.818089	2.351797e-52	1.743427e-49
MsG0180001102.01	MsG0880046237.01	0.815307	9.885321e-52	6.790980e-49
MsG0180001237.01	MsG0880046237.01	0.806453	8.166750e-50	4.434497e-47
MsG0880045294.01	MsG0880046237.01	0.817969	2.502787e-52	1.849293e-49
MsG0880046237.01	MsG0880046238.01	0.913472	5.912722e-84	1.400812e-79
MsG0880046237.01	MsG0880047388.01	0.868689	5.419207e-66	1.978256e-62
MsG0180004991.01	MsG0880046237.01	0.807886	4.059377e-50	2.287477e-47
MsG0180005337.01	MsG0880046237.01	0.821139	4.731286e-53	3.817442e-50
MsG0180005530.01	MsG0880046237.01	0.824427	8.121356e-54	7.190786e-51
MsG0180005746.01	MsG0880046237.01	0.854356	1.299997e-61	2.895313e-58
MsG0180006070.01	MsG0880046237.01	0.800874	1.174501e-48	5.529777e-46
MsG0280006305.01	MsG0880046237.01	0.817727	2.838292e-52	2.083523e-49
MsG0780041209.01	MsG0880046237.01	0.820862	5.481910e-53	4.389094e-50
MsG0780041423.01	MsG0880046237.01	0.837917	3.916269e-57	5.162657e-54
MsG0880042544.01	MsG0880046237.01	0.821902	3.154606e-53	2.600370e-50
MsG0880042760.01	MsG0880046237.01	0.804891	1.737690e-49	9.065938e-47
MsG0880042991.01	MsG0880046237.01	0.808557	2.921479e-50	1.675605e-47
MsG0880043748.01	MsG0880046237.01	0.801533	8.611112e-49	4.122228e-46
MsG0280011089.01	MsG0880046237.01	0.807685	4.480201e-50	2.511428e-47
MsG0380013641.01	MsG0880046237.01	0.833113	6.427187e-56	7.330741e-53
MsG0380014778.01	MsG0880046237.01	0.839692	1.360837e-57	1.893892e-54
MsG0680030721.01	MsG0880046237.01	0.803030	4.233682e-49	2.105352e-46
MsG0680030865.01	MsG0880046237.01	0.840362	9.100093e-58	1.293196e-54
MsG0680030968.01	MsG0880046237.01	0.862285	5.646256e-64	1.644664e-60
MsG0680034497.01	MsG0880046237.01	0.816727	4.765274e-52	3.403297e-49
MsG0680035577.01	MsG0880046237.01	0.800689	1.281295e-48	6.004020e-46
MsG0480020597.01	MsG0880046237.01	0.811162	8.038089e-51	4.939370e-48
MsG0480021823.01	MsG0880046237.01	0.816263	6.050974e-52	4.267416e-49
MsG0480023387.01	MsG0880046237.01	0.857485	1.580818e-62	3.907827e-59
MsG0580025397.01	MsG0880046237.01	0.812677	3.759038e-51	2.405136e-48
MsG0380016873.01	MsG0880046237.01	0.801226	9.948356e-49	4.725713e-46
MsG0380017783.01	MsG0880046237.01	0.819001	1.460824e-52	1.110720e-49
MsG0380017836.01	MsG0880046237.01	0.875216	3.669235e-68	1.699747e-64
MsG0480018335.01	MsG0880046237.01	0.814249	1.696160e-51	1.132289e-48
MsG0580028060.01	MsG0880046237.01	0.812331	4.475016e-51	2.837221e-48
MsG0580028897.01	MsG0880046237.01	0.816889	4.382365e-52	3.143775e-49
MsG0580029371.01	MsG0880046237.01	0.815373	9.559299e-52	6.578944e-49
MsG0280006881.01	MsG0880046237.01	0.810955	8.910864e-51	5.446507e-48
MsG0280007196.01	MsG0880046237.01	0.801453	8.939707e-49	4.270922e-46
MsG0280007226.01	MsG0880046237.01	0.801570	8.458861e-49	4.053108e-46
MsG0280007707.01	MsG0880046237.01	0.804262	2.350165e-49	1.206252e-46
MsG0280008118.01	MsG0880046237.01	0.822001	2.991140e-53	2.472523e-50
MsG0280008283.01	MsG0880046237.01	0.808216	3.453681e-50	1.963317e-47
MsG0280008614.01	MsG0880046237.01	0.814354	1.608293e-51	1.076687e-48
MsG0280009142.01	MsG0880046237.01	0.803603	3.221469e-49	1.625803e-46
MsG0280009451.01	MsG0880046237.01	0.806193	9.262613e-50	4.996393e-47
MsG0280010002.01	MsG0880046237.01	0.809292	2.033510e-50	1.189333e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046237.01.T01	MTR_4g103680	80.460	87	16	1	1	87	180	265	2.99e-43	147
MsG0880046237.01.T01	MTR_5g042880	80.460	87	16	1	1	87	182	267	3.08e-43	147
MsG0880046237.01.T01	MTR_3g088970	72.414	87	23	1	1	87	179	264	3.71e-39	135
MsG0880046237.01.T01	MTR_3g088950	89.474	38	4	0	49	86	512	549	8.44e-20	83.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046237.01.T01	AT4G24830	72.414	87	23	1	1	87	196	281	7.03e-39	134
MsG0880046237.01.T01	AT4G24830	72.414	87	23	1	1	87	196	281	1.24e-38	134

Find 32 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCACAACGTTCCCGTTC+CGG	0.350464	8:-70874443	MsG0880046237.01.T01:CDS
TGGAGGGAGTGGGATATTAC+AGG	0.373658	8:-70874493	MsG0880046237.01.T01:CDS
GGAGCCACAATGTTTAGCTT+GGG	0.388294	8:+70874517	None:intergenic
GTTCCTATCCCTGCTATAGA+TGG	0.391675	8:+70874408	None:intergenic
CTAGTTGTAGTGTTGAATAT+TGG	0.395811	8:-70874334	MsG0880046237.01.T01:CDS
TTCCTTTCCCTGTACACCCA+TGG	0.439674	8:+70874677	None:intergenic
TAGCCATGAGGTATGCTTTG+TGG	0.444924	8:-70874374	MsG0880046237.01.T01:CDS
GCTGTCTCCCATGGGTGTAC+AGG	0.452794	8:-70874685	MsG0880046237.01.T01:CDS
TGGAGCCACAATGTTTAGCT+TGG	0.467832	8:+70874516	None:intergenic
GGAGCTGATGCTGTCTCCCA+TGG	0.468989	8:-70874694	MsG0880046237.01.T01:CDS
TTGTGGCTCCATGGAGGGAG+TGG	0.488472	8:-70874504	MsG0880046237.01.T01:CDS
TACAGGGAAAGGAAATGATC+AGG	0.502638	8:-70874668	MsG0880046237.01.T01:CDS
TCCTTTCCCTGTACACCCAT+GGG	0.506646	8:+70874678	None:intergenic
AGCTAAACATTGTGGCTCCA+TGG	0.517704	8:-70874513	MsG0880046237.01.T01:CDS
GAGCTGATGCTGTCTCCCAT+GGG	0.524079	8:-70874693	MsG0880046237.01.T01:CDS
GTAATATCCCACTCCCTCCA+TGG	0.530801	8:+70874496	None:intergenic
CTGTCTCCCATGGGTGTACA+GGG	0.536193	8:-70874684	MsG0880046237.01.T01:CDS
TCCCATGGGTGTACAGGGAA+AGG	0.539361	8:-70874679	MsG0880046237.01.T01:CDS
AATCCATCTATAGCAGGGAT+AGG	0.548987	8:-70874411	MsG0880046237.01.T01:CDS
GGTGGATGTGCTAAAGAAGT+TGG	0.550891	8:-70874715	MsG0880046237.01.T01:CDS
GAGCCACAATGTTTAGCTTG+GGG	0.558250	8:+70874518	None:intergenic
ATAGCAGGGATAGGAACCTA+TGG	0.560895	8:-70874402	MsG0880046237.01.T01:CDS
AAACATTGTGGCTCCATGGA+GGG	0.593118	8:-70874509	MsG0880046237.01.T01:CDS
CTACAACTAGAGCTAGATTG+AGG	0.594049	8:+70874347	None:intergenic
CCTCATGGCTAAGATGCCAT+AGG	0.600016	8:+70874386	None:intergenic
TGTGGCTCCATGGAGGGAGT+GGG	0.609883	8:-70874503	MsG0880046237.01.T01:CDS
CTTCTTTAGCACATCCACCA+TGG	0.639801	8:+70874719	None:intergenic
TAACCACAAAGCATACCTCA+TGG	0.651316	8:+70874371	None:intergenic
GAACCCCAAGCTAAACATTG+TGG	0.664560	8:-70874521	MsG0880046237.01.T01:CDS
TAAACATTGTGGCTCCATGG+AGG	0.674929	8:-70874510	MsG0880046237.01.T01:CDS
GGAGGGAGTGGGATATTACA+GGG	0.688013	8:-70874492	MsG0880046237.01.T01:CDS
CCTATGGCATCTTAGCCATG+AGG	0.721010	8:-70874386	MsG0880046237.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAAAAATCCATCTATAGCA+GGG	-	Chr8:70874631-70874650	MsG0880046237.01.T01:intron	25.0%
	CAAAAAAATCCATCTATAGC+AGG	-	Chr8:70874630-70874649	MsG0880046237.01.T01:intron	30.0%
!	CTAGTTGTAGTGTTGAATAT+TGG	-	Chr8:70874713-70874732	MsG0880046237.01.T01:CDS	30.0%
!!!	TAGATGGATTTTTTTGTCAC+CGG	+	Chr8:70874626-70874645	None:intergenic	30.0%
!!	GTGCTTTTTAGCATACTCAA+TGG	+	Chr8:70874591-70874610	None:intergenic	35.0%
	AATCCATCTATAGCAGGGAT+AGG	-	Chr8:70874636-70874655	MsG0880046237.01.T01:intron	40.0%
	CTACAACTAGAGCTAGATTG+AGG	+	Chr8:70874703-70874722	None:intergenic	40.0%
	TAACCACAAAGCATACCTCA+TGG	+	Chr8:70874679-70874698	None:intergenic	40.0%
	TACAGGGAAAGGAAATGATC+AGG	-	Chr8:70874379-70874398	MsG0880046237.01.T01:CDS	40.0%
!!!	GATTTTTTTGTCACCGGAAC+GGG	+	Chr8:70874620-70874639	None:intergenic	40.0%
!!!	GGATTTTTTTGTCACCGGAA+CGG	+	Chr8:70874621-70874640	None:intergenic	40.0%
	AAACATTGTGGCTCCATGGA+GGG	-	Chr8:70874538-70874557	MsG0880046237.01.T01:CDS	45.0%
	AGCTAAACATTGTGGCTCCA+TGG	-	Chr8:70874534-70874553	MsG0880046237.01.T01:CDS	45.0%
	ATAGCAGGGATAGGAACCTA+TGG	-	Chr8:70874645-70874664	MsG0880046237.01.T01:intron	45.0%
	GAACCCCAAGCTAAACATTG+TGG	-	Chr8:70874526-70874545	MsG0880046237.01.T01:CDS	45.0%
	GGTGGATGTGCTAAAGAAGT+TGG	-	Chr8:70874332-70874351	MsG0880046237.01.T01:CDS	45.0%
	GTTCCTATCCCTGCTATAGA+TGG	+	Chr8:70874642-70874661	None:intergenic	45.0%
	TAAACATTGTGGCTCCATGG+AGG	-	Chr8:70874537-70874556	MsG0880046237.01.T01:CDS	45.0%
	TAGCCATGAGGTATGCTTTG+TGG	-	Chr8:70874673-70874692	MsG0880046237.01.T01:CDS	45.0%
	TGGAGCCACAATGTTTAGCT+TGG	+	Chr8:70874534-70874553	None:intergenic	45.0%
!	GGAGCCACAATGTTTAGCTT+GGG	+	Chr8:70874533-70874552	None:intergenic	45.0%
!!	GAGCCACAATGTTTAGCTTG+GGG	+	Chr8:70874532-70874551	None:intergenic	45.0%
!!!	TTATTTTTTTATAAACATTT+AGG	-	Chr8:70874475-70874494	MsG0880046237.01.T01:CDS	5.0%
	AAAGCACAACGTTCCCGTTC+CGG	-	Chr8:70874604-70874623	MsG0880046237.01.T01:intron	50.0%
	CCTATGGCATCTTAGCCATG+AGG	-	Chr8:70874661-70874680	MsG0880046237.01.T01:intron	50.0%
	CCTCATGGCTAAGATGCCAT+AGG	+	Chr8:70874664-70874683	None:intergenic	50.0%
	GGAGGGAGTGGGATATTACA+GGG	-	Chr8:70874555-70874574	MsG0880046237.01.T01:intron	50.0%
	GTAATATCCCACTCCCTCCA+TGG	+	Chr8:70874554-70874573	None:intergenic	50.0%
	TCCTTTCCCTGTACACCCAT+GGG	+	Chr8:70874372-70874391	None:intergenic	50.0%
	TGGAGGGAGTGGGATATTAC+AGG	-	Chr8:70874554-70874573	MsG0880046237.01.T01:intron	50.0%
	TTCCTTTCCCTGTACACCCA+TGG	+	Chr8:70874373-70874392	None:intergenic	50.0%
	CTGTCTCCCATGGGTGTACA+GGG	-	Chr8:70874363-70874382	MsG0880046237.01.T01:CDS	55.0%
	GAGCTGATGCTGTCTCCCAT+GGG	-	Chr8:70874354-70874373	MsG0880046237.01.T01:CDS	55.0%
	TCCCATGGGTGTACAGGGAA+AGG	-	Chr8:70874368-70874387	MsG0880046237.01.T01:CDS	55.0%
	GCTGTCTCCCATGGGTGTAC+AGG	-	Chr8:70874362-70874381	MsG0880046237.01.T01:CDS	60.0%
	GGAGCTGATGCTGTCTCCCA+TGG	-	Chr8:70874353-70874372	MsG0880046237.01.T01:CDS	60.0%
	TGTGGCTCCATGGAGGGAGT+GGG	-	Chr8:70874544-70874563	MsG0880046237.01.T01:CDS	60.0%
	TTGTGGCTCCATGGAGGGAG+TGG	-	Chr8:70874543-70874562	MsG0880046237.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	70874330	70874739	70874330	ID=MsG0880046237.01;Name=MsG0880046237.01
Chr8	mRNA	70874330	70874739	70874330	ID=MsG0880046237.01.T01;Parent=MsG0880046237.01;Name=MsG0880046237.01.T01;_AED=0.47;_eAED=0.48;_QI=0\|0\|0\|1\|1\|0.5\|2\|0\|105
Chr8	exon	70874665	70874739	70874665	ID=MsG0880046237.01.T01:exon:15339;Parent=MsG0880046237.01.T01
Chr8	exon	70874330	70874572	70874330	ID=MsG0880046237.01.T01:exon:15338;Parent=MsG0880046237.01.T01
Chr8	CDS	70874665	70874739	70874665	ID=MsG0880046237.01.T01:cds;Parent=MsG0880046237.01.T01
Chr8	CDS	70874330	70874572	70874330	ID=MsG0880046237.01.T01:cds;Parent=MsG0880046237.01.T01

Gene Sequence	>MsG0880046237.01.T01 ATGGTGGATGTGCTAAAGAAGTTGGAGCTGATGCTGTCTCCCATGGGTGTACAGGGAAAGGAAATGATCAGGCATGTTCGATTTGAGCTTACTTTCTTTGCGCTGAACCCCAAGCTAAACATTGTGGCTCCATGGAGGGAGTGGGATATTACAGGGAGAGAAGATGCCATTGAGTATGCTAAAAAGCACAACGTTCCCGTTCCGGTGACAAAAAAATCCATCTATAGCAGGGATAGGAACCTATGGCATCTTAGCCATGAGGTATGCTTTGTGGTTATCCTCAATCTAGCTCTAGTTGTAGTGTTGAATATTGGCTGA
Protein sequence	>MsG0880046237.01.T01 MVDVLKKLELMLSPMGVQGKEMIRHVRFELTFFALNPKLNIVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLWHLSHEVCFVVILNLALVVVLNIG*