AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280009530.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009530.01.T01 MTR_7g024060 92.437 238 18 0 2 239 1 238 1.64e-167 465
MsG0280009530.01.T01 MTR_6g087820 92.437 238 18 0 2 239 1 238 1.64e-167 465
MsG0280009530.01.T01 MTR_5g081190 92.437 238 18 0 2 239 1 238 1.64e-167 465
MsG0280009530.01.T01 MTR_5g091480 92.437 238 18 0 2 239 1 238 1.64e-167 465
MsG0280009530.01.T01 MTR_5g056290 92.437 238 18 0 2 239 1 238 1.64e-167 465
MsG0280009530.01.T01 MTR_3g031190 92.437 238 18 0 2 239 1 238 1.64e-167 465
MsG0280009530.01.T01 MTR_2g005490 92.437 238 18 0 2 239 1 238 1.64e-167 465
MsG0280009530.01.T01 MTR_2g010570 92.437 238 18 0 2 239 1 238 1.64e-167 465
MsG0280009530.01.T01 MTR_1g023370 92.437 238 18 0 2 239 1 238 7.37e-167 462
MsG0280009530.01.T01 MTR_5g030110 92.017 238 19 0 2 239 1 238 9.29e-167 463
MsG0280009530.01.T01 MTR_2g007410 92.017 238 19 0 2 239 1 238 9.29e-167 463
MsG0280009530.01.T01 MTR_7g074180 92.017 238 19 0 2 239 1 238 1.18e-166 463
MsG0280009530.01.T01 MTR_7g451880 91.213 239 21 0 1 239 1 239 2.50e-165 459
MsG0280009530.01.T01 MTR_3g015660 91.597 238 20 0 2 239 1 238 3.37e-165 456
MsG0280009530.01.T01 MTR_4g058660 91.525 236 20 0 4 239 3 238 1.37e-163 455
MsG0280009530.01.T01 MTR_1g011490 88.235 238 19 1 2 239 1 229 7.09e-157 438
MsG0280009530.01.T01 MTR_1g082860 89.700 233 15 1 2 234 1 224 4.22e-155 432
MsG0280009530.01.T01 MTR_8g446640 92.694 219 16 0 21 239 1 219 2.88e-154 431
MsG0280009530.01.T01 MTR_2g010270 88.596 228 17 1 21 239 1 228 2.58e-152 426
MsG0280009530.01.T01 MTR_5g045850 80.672 238 24 3 2 239 1 216 1.33e-137 388
MsG0280009530.01.T01 MTR_8g005940 79.832 238 15 1 2 239 1 205 5.48e-137 387
MsG0280009530.01.T01 MTR_0105s0080 80.734 218 17 1 21 238 1 193 2.65e-124 353
MsG0280009530.01.T01 MTR_5g012180 69.328 238 24 4 2 239 1 189 1.26e-112 324
MsG0280009530.01.T01 MTR_3g036220 66.977 215 26 3 2 216 1 170 6.05e-98 286
MsG0280009530.01.T01 MTR_5g071020 59.244 238 19 4 2 239 1 160 3.49e-87 258
MsG0280009530.01.T01 MTR_7g045450 53.586 237 95 1 2 238 1 222 5.69e-85 253
MsG0280009530.01.T01 MTR_6g073570 53.586 237 95 1 2 238 1 222 5.69e-85 253
MsG0280009530.01.T01 MTR_1g105785 46.522 230 123 0 7 236 6 235 1.33e-75 239
MsG0280009530.01.T01 MTR_5g019960 51.562 192 91 2 48 238 53 243 6.23e-70 215
MsG0280009530.01.T01 MTR_3g452170 44.105 229 128 0 7 235 6 234 1.14e-69 221
MsG0280009530.01.T01 MTR_1g110330 72.028 143 17 1 1 143 1 120 2.11e-68 209
MsG0280009530.01.T01 MTR_7g110100 43.668 229 129 0 7 235 6 234 3.27e-68 219
MsG0280009530.01.T01 MTR_3g449160 70.629 143 12 1 70 212 1 113 3.71e-67 203
MsG0280009530.01.T01 MTR_2g435900 71.329 143 11 1 70 212 1 113 5.32e-67 202
MsG0280009530.01.T01 MTR_1g052715 40.909 198 116 1 43 239 1 198 3.17e-55 180
MsG0280009530.01.T01 MTR_7g051400 40.092 217 125 1 7 223 6 217 1.41e-54 183
MsG0280009530.01.T01 MTR_2g019040 90.698 86 8 0 153 238 4 89 1.84e-52 170
MsG0280009530.01.T01 MTR_3g007260 46.951 164 61 2 52 215 36 173 5.81e-49 159
MsG0280009530.01.T01 MTR_7g039300 48.045 179 62 1 2 149 1 179 1.06e-48 160
MsG0280009530.01.T01 MTR_1g101230 86.813 91 9 2 2 89 1 91 8.44e-48 156
MsG0280009530.01.T01 MTR_8g465040 43.976 166 93 0 7 172 6 171 2.59e-44 147
MsG0280009530.01.T01 MTR_6g465570 41.916 167 97 0 7 173 2 168 9.08e-44 145
MsG0280009530.01.T01 MTR_1g051235 43.114 167 94 1 7 172 6 172 2.35e-40 137
MsG0280009530.01.T01 MTR_7g070110 59.292 113 42 1 20 132 7 115 3.24e-40 135
MsG0280009530.01.T01 MTR_6g069460 70.652 92 27 0 17 108 50 141 1.13e-39 134
MsG0280009530.01.T01 MTR_6g024030 56.250 96 42 0 141 236 60 155 7.19e-34 122
MsG0280009530.01.T01 MTR_6g024030 71.698 53 15 0 6 58 5 57 7.67e-20 85.5
MsG0280009530.01.T01 MTR_0048s0080 56.122 98 43 0 140 237 5 102 1.42e-33 124
MsG0280009530.01.T01 MTR_7g096440 30.675 163 113 0 54 216 6 168 1.07e-30 114
MsG0280009530.01.T01 MTR_7g051840 39.062 128 75 1 45 172 1 125 4.38e-30 108
MsG0280009530.01.T01 MTR_8g024070 62.500 80 30 0 6 85 5 84 1.07e-29 106
MsG0280009530.01.T01 MTR_4g017450 42.308 130 74 1 7 136 6 134 1.09e-28 105
MsG0280009530.01.T01 MTR_8g463820 26.778 239 153 5 2 236 1 221 1.91e-27 106
MsG0280009530.01.T01 MTR_6g039240 26.778 239 153 5 2 236 1 221 2.56e-27 106
MsG0280009530.01.T01 MTR_1g094230 26.778 239 153 5 2 236 1 221 9.44e-27 105
MsG0280009530.01.T01 MTR_4g036240 26.872 227 163 3 6 231 5 229 3.56e-26 106
MsG0280009530.01.T01 MTR_2g450060 28.326 233 160 4 6 236 9 236 4.11e-24 100
MsG0280009530.01.T01 MTR_3g056270 29.630 189 131 2 6 193 5 192 5.13e-24 100
MsG0280009530.01.T01 MTR_3g056270 27.179 195 139 3 38 231 196 388 2.42e-20 89.7
MsG0280009530.01.T01 MTR_3g056240 27.273 220 155 4 6 223 5 221 7.71e-24 97.8
MsG0280009530.01.T01 MTR_4g014890 27.083 240 167 4 2 238 28 262 9.45e-24 99.4
MsG0280009530.01.T01 MTR_8g088810 26.971 241 163 6 2 236 1 234 1.49e-23 96.7
MsG0280009530.01.T01 MTR_3g055660 26.667 240 168 4 2 238 28 262 3.17e-23 98.2
MsG0280009530.01.T01 MTR_8g062030 26.991 226 162 3 15 239 14 237 6.93e-23 97.1
MsG0280009530.01.T01 MTR_2g048560 23.849 239 176 4 2 239 1 234 9.72e-23 96.7
MsG0280009530.01.T01 MTR_6g059860 27.083 240 167 4 2 238 22 256 1.68e-22 95.9
MsG0280009530.01.T01 MTR_2g008650 25.862 232 163 5 3 230 4 230 2.43e-22 95.5
MsG0280009530.01.T01 MTR_6g055250 30.964 197 126 4 45 234 1 194 2.96e-22 94.4
MsG0280009530.01.T01 MTR_8g479260 25.862 232 163 5 3 230 4 230 4.36e-22 94.7
MsG0280009530.01.T01 MTR_5g078250 25.000 224 165 3 9 231 8 229 7.45e-22 94.0
MsG0280009530.01.T01 MTR_2g054710 24.569 232 166 5 3 230 4 230 1.23e-21 90.9
MsG0280009530.01.T01 MTR_7g089660 26.442 208 132 4 2 205 1 191 2.59e-21 89.4
MsG0280009530.01.T01 MTR_1g043690 29.493 217 142 4 20 234 26 233 4.23e-21 92.0
MsG0280009530.01.T01 MTR_5g053590 31.674 221 139 7 20 237 19 230 4.80e-21 91.7
MsG0280009530.01.T01 MTR_3g116750 31.579 171 108 4 2 166 1 168 1.11e-20 85.9
MsG0280009530.01.T01 MTR_4g085760 27.111 225 149 6 21 238 26 242 1.51e-20 90.5
MsG0280009530.01.T01 MTR_4g471610 31.579 171 108 4 2 166 1 168 1.54e-20 85.5
MsG0280009530.01.T01 MTR_3g078440 31.579 171 108 4 2 166 1 168 1.54e-20 85.5
MsG0280009530.01.T01 MTR_8g036090 31.579 171 108 4 2 166 1 168 1.54e-20 85.5
MsG0280009530.01.T01 MTR_1g036740 31.579 171 108 4 2 166 1 168 1.74e-20 85.5
MsG0280009530.01.T01 MTR_8g096680 31.579 171 108 4 2 166 1 168 1.74e-20 85.5
MsG0280009530.01.T01 MTR_5g085220 30.357 168 114 2 2 166 1 168 2.54e-20 85.1
MsG0280009530.01.T01 MTR_5g004860 29.279 222 144 7 20 236 19 232 4.92e-20 89.0
MsG0280009530.01.T01 MTR_3g095950 30.994 171 109 4 2 166 1 168 5.52e-20 84.3
MsG0280009530.01.T01 MTR_6g052340 29.730 222 143 7 20 236 21 234 6.85e-20 88.6
MsG0280009530.01.T01 MTR_8g027910 28.632 234 155 5 9 236 18 245 3.19e-19 84.3
MsG0280009530.01.T01 MTR_1g075850 25.664 226 155 6 9 231 8 223 8.46e-19 85.1
MsG0280009530.01.T01 MTR_7g093810 30.409 171 110 4 2 166 1 168 9.58e-19 80.9
MsG0280009530.01.T01 MTR_5g055160 30.247 162 106 3 2 159 1 159 1.57e-18 82.4
MsG0280009530.01.T01 MTR_2g062200 25.000 172 126 3 45 215 1 170 5.87e-18 79.3
MsG0280009530.01.T01 MTR_6g464080 28.934 197 133 5 43 235 39 232 5.42e-17 80.1
MsG0280009530.01.T01 MTR_2g032570 28.324 173 113 4 2 166 1 170 3.83e-16 73.9
MsG0280009530.01.T01 MTR_4g028320 28.859 149 103 2 21 166 1 149 5.06e-16 73.2
MsG0280009530.01.T01 MTR_4g070090 27.126 247 164 6 6 238 20 264 1.03e-15 76.3
MsG0280009530.01.T01 MTR_7g025160 28.155 206 141 3 9 211 18 219 1.53e-15 73.9
MsG0280009530.01.T01 MTR_6g045290 29.268 164 114 2 49 211 302 464 1.75e-15 75.5
MsG0280009530.01.T01 MTR_6g013455 23.554 242 140 6 2 236 1 204 1.13e-14 72.0
MsG0280009530.01.T01 MTR_4g070160 26.136 176 127 3 63 236 1 175 1.14e-14 70.9
MsG0280009530.01.T01 MTR_3g084550 30.709 127 85 2 43 166 1 127 1.35e-14 68.9
MsG0280009530.01.T01 MTR_2g048550 25.431 232 160 6 6 231 5 229 3.13e-14 71.6
MsG0280009530.01.T01 MTR_1g072220 30.709 127 85 2 43 166 1 127 3.72e-14 67.4
MsG0280009530.01.T01 MTR_2g099880 32.558 129 84 2 41 166 5 133 1.88e-13 65.9
MsG0280009530.01.T01 MTR_2g055290 26.471 204 118 6 9 211 8 180 3.50e-13 66.2
MsG0280009530.01.T01 MTR_8g076570 26.484 219 145 7 20 231 19 228 4.00e-13 68.9
MsG0280009530.01.T01 MTR_0003s0290 29.814 161 110 3 81 239 44 203 4.21e-13 68.6
MsG0280009530.01.T01 MTR_7g104000 28.659 164 98 4 2 158 1 152 1.66e-12 65.5
MsG0280009530.01.T01 MTR_6g051890 30.769 130 89 1 15 143 18 147 4.46e-12 65.5
MsG0280009530.01.T01 MTR_4g016120 28.571 147 94 4 24 166 20 159 4.80e-12 62.8
MsG0280009530.01.T01 MTR_5g080500 40.426 94 55 1 7 99 6 99 4.92e-12 62.4
MsG0280009530.01.T01 MTR_5g056060 27.545 167 102 4 2 161 1 155 5.52e-12 63.9
MsG0280009530.01.T01 MTR_1788s0010 29.104 134 85 3 2 128 1 131 5.92e-12 62.0
MsG0280009530.01.T01 MTR_7g052740 25.888 197 123 4 43 236 109 285 6.34e-12 64.7
MsG0280009530.01.T01 MTR_3g044760 37.374 99 57 2 8 102 4 101 1.23e-11 63.9
MsG0280009530.01.T01 MTR_3g068135 84.848 33 5 0 140 172 6 38 3.89e-11 60.8
MsG0280009530.01.T01 MTR_8g467450 29.412 119 74 2 6 119 5 118 5.82e-11 59.3
MsG0280009530.01.T01 MTR_1g011320 27.879 165 110 6 52 212 62 221 8.18e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009530.01.T01 AT1G52950 26.609 233 163 5 6 236 7 233 8.20e-21 91.3
MsG0280009530.01.T01 AT2G05642 24.038 208 120 2 7 214 6 175 2.15e-20 89.7

Find 57 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAACTCGCACACACATTT+TGG 0.155023 2:-53832278 None:intergenic
GACAAAATTGTGACCTTTAT+TGG 0.169025 2:+53833099 MsG0280009530.01.T01:CDS
AGCTGAGATTGGAGTGTATT+TGG 0.198780 2:-53832233 None:intergenic
CCACTTGCATTGCTCTCTTT+GGG 0.202399 2:+53833065 MsG0280009530.01.T01:CDS
ACCACTTGCATTGCTCTCTT+TGG 0.221388 2:+53833064 MsG0280009530.01.T01:CDS
GGAGTTGCTAATGCCTTTAA+TGG 0.246567 2:+53833286 MsG0280009530.01.T01:CDS
TTGTGTGAAGTTACCTTATA+AGG 0.258341 2:-53832596 None:intergenic
TGCAAGACACGCATGTATTT+AGG 0.278275 2:+53833165 MsG0280009530.01.T01:CDS
ACTCCAATCTCAGCTGTTTC+TGG 0.285991 2:+53832241 MsG0280009530.01.T01:CDS
TATCAGCTTAGTCCCATTAA+AGG 0.310808 2:-53833299 None:intergenic
TAATTGAAAGAGGTGATATA+AGG 0.328150 2:+53832881 MsG0280009530.01.T01:CDS
GGGACTAAGCTGATACTTAA+TGG 0.330761 2:+53833307 MsG0280009530.01.T01:CDS
TACGTAATTTCAGATGTTAT+TGG 0.335367 2:+53832853 MsG0280009530.01.T01:intron
CGCCACAGCTAGAAAAGATT+TGG 0.338595 2:+53832496 MsG0280009530.01.T01:CDS
CAGCTTGAAAATGCAATTGT+AGG 0.343242 2:+53832560 MsG0280009530.01.T01:CDS
GTCATTTACGCAGCAAATTC+AGG 0.347176 2:-53833337 None:intergenic
GGTTATTGATCTCACTTTAG+AGG 0.347534 2:+53832933 MsG0280009530.01.T01:CDS
AAGATTTGGTGGCAAGGTTC+AGG 0.355625 2:+53832510 MsG0280009530.01.T01:CDS
TTATAATGAAGGTCCTTATA+AGG 0.381050 2:+53832583 MsG0280009530.01.T01:CDS
GTTGTTATGGTTACCAATAA+AGG 0.396551 2:-53833112 None:intergenic
AACTGTCGAGGAGGTGTCTA+CGG 0.418179 2:+53832724 MsG0280009530.01.T01:CDS
CATGTTACTGGTCGATGAAA+AGG 0.418554 2:+53832354 MsG0280009530.01.T01:CDS
TAGTAGGTGTGTGGTTGTTA+TGG 0.420451 2:-53833125 None:intergenic
GAAGAGAGAATTTCATTGAA+TGG 0.432380 2:-53832701 None:intergenic
GAGTTGCTAATGCCTTTAAT+GGG 0.439194 2:+53833287 MsG0280009530.01.T01:CDS
CCTCCAGAAACAGCTGAGAT+TGG 0.442150 2:-53832244 None:intergenic
GGTGGCAAGGTTCAGGTCCA+TGG 0.442231 2:+53832517 MsG0280009530.01.T01:CDS
CACTTGCATTGCTCTCTTTG+GGG 0.462664 2:+53833066 MsG0280009530.01.T01:CDS
TTGGTTGATTCGAGAGAAGA+AGG 0.473139 2:+53832306 MsG0280009530.01.T01:CDS
TTCAGGTCCATGGTTGAAGA+AGG 0.473259 2:+53832527 MsG0280009530.01.T01:CDS
AATTGAAAGAGGTGATATAA+GGG 0.489125 2:+53832882 MsG0280009530.01.T01:CDS
AAAAGGATGGAAGGAAAAGC+AGG 0.493666 2:+53832911 MsG0280009530.01.T01:CDS
GATAGCAGGCGAGTGTACTT+TGG 0.506539 2:-53832654 None:intergenic
GTGTATTTGGCAGATATCAT+TGG 0.512275 2:-53832220 None:intergenic
AACTGCAATATCAATATAGT+AGG 0.513997 2:-53833141 None:intergenic
TGATATAAGGGAAACTGAAA+AGG 0.524272 2:+53832894 MsG0280009530.01.T01:CDS
GCAATTGTAGGTTATAATGA+AGG 0.529492 2:+53832572 MsG0280009530.01.T01:CDS
ATAAGGGAAACTGAAAAGGA+TGG 0.532230 2:+53832898 MsG0280009530.01.T01:CDS
ATTGGTCATGTAATTGAAAG+AGG 0.535259 2:+53832871 MsG0280009530.01.T01:CDS
GTGTGCGAGTTGCTCACATT+TGG 0.538560 2:+53832287 MsG0280009530.01.T01:CDS
AAAACATTCATAGGGATAGC+AGG 0.542314 2:-53832668 None:intergenic
CAGGTCCATGGTTGAAGAAG+GGG 0.549020 2:+53832529 MsG0280009530.01.T01:CDS
TCAGGTCCATGGTTGAAGAA+GGG 0.552024 2:+53832528 MsG0280009530.01.T01:CDS
ACTGTCGAGGAGGTGTCTAC+GGG 0.565078 2:+53832725 MsG0280009530.01.T01:CDS
CACAGCTAGAAAAGATTTGG+TGG 0.574260 2:+53832499 MsG0280009530.01.T01:CDS
GGGAAACTGAAAAGGATGGA+AGG 0.581802 2:+53832902 MsG0280009530.01.T01:CDS
CTAGAAAAGATTTGGTGGCA+AGG 0.606708 2:+53832504 MsG0280009530.01.T01:CDS
TTCATGAAAATGATGCTAGT+GGG 0.608107 2:-53832331 None:intergenic
AATTCTCTCTTCAACTGTCG+AGG 0.611208 2:+53832712 MsG0280009530.01.T01:CDS
CCCAAAGAGAGCAATGCAAG+TGG 0.621311 2:-53833065 None:intergenic
CCAATCTCAGCTGTTTCTGG+AGG 0.626389 2:+53832244 MsG0280009530.01.T01:CDS
GTTCATGAAAATGATGCTAG+TGG 0.636086 2:-53832332 None:intergenic
TCTCTCTTCAACTGTCGAGG+AGG 0.649892 2:+53832715 MsG0280009530.01.T01:CDS
AGGTCCATGGTTGAAGAAGG+GGG 0.671399 2:+53832530 MsG0280009530.01.T01:CDS
AAAGGATGGAAGGAAAAGCA+GGG 0.682942 2:+53832912 MsG0280009530.01.T01:CDS
TCATGAAAATGATGCTAGTG+GGG 0.712129 2:-53832330 None:intergenic
ATATCAATATAGTAGGTGTG+TGG 0.756687 2:-53833134 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTGAATTTTTGAAAAATT+AGG + Chr2:53833256-53833275 MsG0280009530.01.T01:intron 15.0%
!! TATTACCAAAATATCAATTC+AGG - Chr2:53833028-53833047 None:intergenic 20.0%
! AACTGCAATATCAATATAGT+AGG - Chr2:53833144-53833163 None:intergenic 25.0%
! AATATCAATTCAGGAACATA+AGG - Chr2:53833019-53833038 None:intergenic 25.0%
! AATTGAAAGAGGTGATATAA+GGG + Chr2:53832882-53832901 MsG0280009530.01.T01:CDS 25.0%
! CAAATGTATGAAATTCCTAA+AGG - Chr2:53832812-53832831 None:intergenic 25.0%
! TAATTGAAAGAGGTGATATA+AGG + Chr2:53832881-53832900 MsG0280009530.01.T01:CDS 25.0%
! TACGTAATTTCAGATGTTAT+TGG + Chr2:53832853-53832872 MsG0280009530.01.T01:intron 25.0%
! TGAACTCAAAAACATTCATA+GGG - Chr2:53832679-53832698 None:intergenic 25.0%
! TTATAATGAAGGTCCTTATA+AGG + Chr2:53832583-53832602 MsG0280009530.01.T01:CDS 25.0%
! TTGAACTCAAAAACATTCAT+AGG - Chr2:53832680-53832699 None:intergenic 25.0%
!! ATGTTCCTGAATTGATATTT+TGG + Chr2:53833020-53833039 MsG0280009530.01.T01:intron 25.0%
!! TTTGAAAAATTAGGTGCTAT+GGG + Chr2:53833265-53833284 MsG0280009530.01.T01:intron 25.0%
!!! GTATGTCATGTTTTAAGTTT+GGG + Chr2:53833187-53833206 MsG0280009530.01.T01:intron 25.0%
!!! TTTTGAAAAATTAGGTGCTA+TGG + Chr2:53833264-53833283 MsG0280009530.01.T01:intron 25.0%
ATATCAATATAGTAGGTGTG+TGG - Chr2:53833137-53833156 None:intergenic 30.0%
ATTCCTAAAGGAATGCTTAT+AGG - Chr2:53832800-53832819 None:intergenic 30.0%
ATTGGTCATGTAATTGAAAG+AGG + Chr2:53832871-53832890 MsG0280009530.01.T01:CDS 30.0%
GAAGAGAGAATTTCATTGAA+TGG - Chr2:53832704-53832723 None:intergenic 30.0%
GACAAAATTGTGACCTTTAT+TGG + Chr2:53833099-53833118 MsG0280009530.01.T01:CDS 30.0%
GCAATTGTAGGTTATAATGA+AGG + Chr2:53832572-53832591 MsG0280009530.01.T01:CDS 30.0%
GTTGTTATGGTTACCAATAA+AGG - Chr2:53833115-53833134 None:intergenic 30.0%
TGATATAAGGGAAACTGAAA+AGG + Chr2:53832894-53832913 MsG0280009530.01.T01:CDS 30.0%
TTCATGAAAATGATGCTAGT+GGG - Chr2:53832334-53832353 None:intergenic 30.0%
TTGTGTGAAGTTACCTTATA+AGG - Chr2:53832599-53832618 None:intergenic 30.0%
! CATTTTCATGAACATGTTAC+TGG + Chr2:53832342-53832361 MsG0280009530.01.T01:CDS 30.0%
! TTTGTCTGTTTCTTTGTATC+TGG + Chr2:53832969-53832988 MsG0280009530.01.T01:intron 30.0%
!!! GGTATGTCATGTTTTAAGTT+TGG + Chr2:53833186-53833205 MsG0280009530.01.T01:intron 30.0%
AAAACATTCATAGGGATAGC+AGG - Chr2:53832671-53832690 None:intergenic 35.0%
ATAAGGGAAACTGAAAAGGA+TGG + Chr2:53832898-53832917 MsG0280009530.01.T01:CDS 35.0%
CAGCTTGAAAATGCAATTGT+AGG + Chr2:53832560-53832579 MsG0280009530.01.T01:CDS 35.0%
CTACCTATAAGCATTCCTTT+AGG + Chr2:53832794-53832813 MsG0280009530.01.T01:intron 35.0%
GAGTTGCTAATGCCTTTAAT+GGG + Chr2:53833287-53833306 MsG0280009530.01.T01:CDS 35.0%
GGTTATTGATCTCACTTTAG+AGG + Chr2:53832933-53832952 MsG0280009530.01.T01:CDS 35.0%
GTTCATGAAAATGATGCTAG+TGG - Chr2:53832335-53832354 None:intergenic 35.0%
TATCAGCTTAGTCCCATTAA+AGG - Chr2:53833302-53833321 None:intergenic 35.0%
TCATGAAAATGATGCTAGTG+GGG - Chr2:53832333-53832352 None:intergenic 35.0%
TGATGTTGTTGTCGTCAATA+GGG + Chr2:53832461-53832480 MsG0280009530.01.T01:intron 35.0%
AAAAGGATGGAAGGAAAAGC+AGG + Chr2:53832911-53832930 MsG0280009530.01.T01:CDS 40.0%
AAAGGATGGAAGGAAAAGCA+GGG + Chr2:53832912-53832931 MsG0280009530.01.T01:CDS 40.0%
AATTCTCTCTTCAACTGTCG+AGG + Chr2:53832712-53832731 MsG0280009530.01.T01:CDS 40.0%
AGCTGAGATTGGAGTGTATT+TGG - Chr2:53832236-53832255 None:intergenic 40.0%
CAACAACATCAGTAAACAGC+TGG - Chr2:53832452-53832471 None:intergenic 40.0%
CACAGCTAGAAAAGATTTGG+TGG + Chr2:53832499-53832518 MsG0280009530.01.T01:CDS 40.0%
CATGTTACTGGTCGATGAAA+AGG + Chr2:53832354-53832373 MsG0280009530.01.T01:CDS 40.0%
CTGATGTTGTTGTCGTCAAT+AGG + Chr2:53832460-53832479 MsG0280009530.01.T01:intron 40.0%
GGAGTTGCTAATGCCTTTAA+TGG + Chr2:53833286-53833305 MsG0280009530.01.T01:CDS 40.0%
GGGACTAAGCTGATACTTAA+TGG + Chr2:53833307-53833326 MsG0280009530.01.T01:CDS 40.0%
TAGTAGGTGTGTGGTTGTTA+TGG - Chr2:53833128-53833147 None:intergenic 40.0%
TGCAAGACACGCATGTATTT+AGG + Chr2:53833165-53833184 MsG0280009530.01.T01:CDS 40.0%
!! CTAGAAAAGATTTGGTGGCA+AGG + Chr2:53832504-53832523 MsG0280009530.01.T01:CDS 40.0%
!! TTGGTTGATTCGAGAGAAGA+AGG + Chr2:53832306-53832325 MsG0280009530.01.T01:CDS 40.0%
!!! CACCAAATCTTTTCTAGCTG+TGG - Chr2:53832501-53832520 None:intergenic 40.0%
ACCACTTGCATTGCTCTCTT+TGG + Chr2:53833064-53833083 MsG0280009530.01.T01:CDS 45.0%
CACTTGCATTGCTCTCTTTG+GGG + Chr2:53833066-53833085 MsG0280009530.01.T01:CDS 45.0%
CCACTTGCATTGCTCTCTTT+GGG + Chr2:53833065-53833084 MsG0280009530.01.T01:CDS 45.0%
CGCCACAGCTAGAAAAGATT+TGG + Chr2:53832496-53832515 MsG0280009530.01.T01:CDS 45.0%
GGGAAACTGAAAAGGATGGA+AGG + Chr2:53832902-53832921 MsG0280009530.01.T01:CDS 45.0%
TCAGGTCCATGGTTGAAGAA+GGG + Chr2:53832528-53832547 MsG0280009530.01.T01:CDS 45.0%
TGTTGTTGTCGTCAATAGGG+TGG + Chr2:53832464-53832483 MsG0280009530.01.T01:intron 45.0%
TTCAGGTCCATGGTTGAAGA+AGG + Chr2:53832527-53832546 MsG0280009530.01.T01:CDS 45.0%
! ACTCCAATCTCAGCTGTTTC+TGG + Chr2:53832241-53832260 MsG0280009530.01.T01:CDS 45.0%
! AGCAACTCGCACACACATTT+TGG - Chr2:53832281-53832300 None:intergenic 45.0%
! CAACTCGCACACACATTTTG+GGG - Chr2:53832279-53832298 None:intergenic 45.0%
! GCAACTCGCACACACATTTT+GGG - Chr2:53832280-53832299 None:intergenic 45.0%
!! AAGATTTGGTGGCAAGGTTC+AGG + Chr2:53832510-53832529 MsG0280009530.01.T01:CDS 45.0%
AGGTCCATGGTTGAAGAAGG+GGG + Chr2:53832530-53832549 MsG0280009530.01.T01:CDS 50.0%
CAGGTCCATGGTTGAAGAAG+GGG + Chr2:53832529-53832548 MsG0280009530.01.T01:CDS 50.0%
CCCAAAGAGAGCAATGCAAG+TGG - Chr2:53833068-53833087 None:intergenic 50.0%
CCTCCAGAAACAGCTGAGAT+TGG - Chr2:53832247-53832266 None:intergenic 50.0%
GATAGCAGGCGAGTGTACTT+TGG - Chr2:53832657-53832676 None:intergenic 50.0%
GTGTGCGAGTTGCTCACATT+TGG + Chr2:53832287-53832306 MsG0280009530.01.T01:CDS 50.0%
TCTCTCTTCAACTGTCGAGG+AGG + Chr2:53832715-53832734 MsG0280009530.01.T01:CDS 50.0%
TGTACCCCCTTCTTCAACCA+TGG - Chr2:53832537-53832556 None:intergenic 50.0%
!! AACTGTCGAGGAGGTGTCTA+CGG + Chr2:53832724-53832743 MsG0280009530.01.T01:CDS 50.0%
!! CCAATCTCAGCTGTTTCTGG+AGG + Chr2:53832244-53832263 MsG0280009530.01.T01:CDS 50.0%
!! ACTGTCGAGGAGGTGTCTAC+GGG + Chr2:53832725-53832744 MsG0280009530.01.T01:CDS 55.0%
GGTGGCAAGGTTCAGGTCCA+TGG + Chr2:53832517-53832536 MsG0280009530.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 53832223 53833354 53832223 ID=MsG0280009530.01;Name=MsG0280009530.01
Chr2 mRNA 53832223 53833354 53832223 ID=MsG0280009530.01.T01;Parent=MsG0280009530.01;Name=MsG0280009530.01.T01;_AED=0.35;_eAED=0.35;_QI=0|0|0|1|1|1|5|0|239
Chr2 exon 53832223 53832375 53832223 ID=MsG0280009530.01.T01:exon:38152;Parent=MsG0280009530.01.T01
Chr2 exon 53832482 53832746 53832482 ID=MsG0280009530.01.T01:exon:38151;Parent=MsG0280009530.01.T01
Chr2 exon 53832866 53832962 53832866 ID=MsG0280009530.01.T01:exon:38150;Parent=MsG0280009530.01.T01
Chr2 exon 53833059 53833186 53833059 ID=MsG0280009530.01.T01:exon:38149;Parent=MsG0280009530.01.T01
Chr2 exon 53833278 53833354 53833278 ID=MsG0280009530.01.T01:exon:38148;Parent=MsG0280009530.01.T01
Chr2 CDS 53832223 53832375 53832223 ID=MsG0280009530.01.T01:cds;Parent=MsG0280009530.01.T01
Chr2 CDS 53832482 53832746 53832482 ID=MsG0280009530.01.T01:cds;Parent=MsG0280009530.01.T01
Chr2 CDS 53832866 53832962 53832866 ID=MsG0280009530.01.T01:cds;Parent=MsG0280009530.01.T01
Chr2 CDS 53833059 53833186 53833059 ID=MsG0280009530.01.T01:cds;Parent=MsG0280009530.01.T01
Chr2 CDS 53833278 53833354 53833278 ID=MsG0280009530.01.T01:cds;Parent=MsG0280009530.01.T01
Gene Sequence

>MsG0280009530.01.T01

ATGATATCTGCCAAATACACTCCAATCTCAGCTGTTTCTGGAGGAAGAAAGAACCCCAAAATGTGTGTGCGAGTTGCTCACATTTGGTTGATTCGAGAGAAGAAGGTCCCCACTAGCATCATTTTCATGAACATGTTACTGGTCGATGAAAAGGGTGGACGTATTCACGCCACAGCTAGAAAAGATTTGGTGGCAAGGTTCAGGTCCATGGTTGAAGAAGGGGGTACATATCAGCTTGAAAATGCAATTGTAGGTTATAATGAAGGTCCTTATAAGGTAACTTCACACAAACATAAGCTTAGTATGATGCACAATTCAACTTTTACCAAAGTACACTCGCCTGCTATCCCTATGAATGTTTTTGAGTTCAAGCCATTCAATGAAATTCTCTCTTCAACTGTCGAGGAGGTGTCTACGGATGTTATTGGTCATGTAATTGAAAGAGGTGATATAAGGGAAACTGAAAAGGATGGAAGGAAAAGCAGGGTTATTGATCTCACTTTAGAGGATCTTGAAAACAACCACTTGCATTGCTCTCTTTGGGGTGAACATGCTGACAAAATTGTGACCTTTATTGGTAACCATAACAACCACACACCTACTATATTGATATTGCAGTTCTGCAAGACACGCATGTATTTAGGTGCTATGGGAGTTGCTAATGCCTTTAATGGGACTAAGCTGATACTTAATGGCGATTTGCCTGAATTTGCTGCGTAA
Protein sequence

>MsG0280009530.01.T01

MISAKYTPISAVSGGRKNPKMCVRVAHIWLIREKKVPTSIIFMNMLLVDEKGGRIHATARKDLVARFRSMVEEGGTYQLENAIVGYNEGPYKVTSHKHKLSMMHNSTFTKVHSPAIPMNVFEFKPFNEILSSTVEEVSTDVIGHVIERGDIRETEKDGRKSRVIDLTLEDLENNHLHCSLWGEHADKIVTFIGNHNNHTPTILILQFCKTRMYLGAMGVANAFNGTKLILNGDLPEFAA*