AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280009613.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009613.01.T01 MTR_8g075320 68.528 197 22 5 1 158 1 196 1.04e-79 240
MsG0280009613.01.T01 MTR_2g016050 62.944 197 33 5 1 158 1 196 4.10e-73 224
MsG0280009613.01.T01 MTR_7g070070 30.052 193 96 3 3 156 4 196 1.04e-26 104
MsG0280009613.01.T01 MTR_5g016590 30.052 193 96 3 3 156 4 196 3.27e-26 103
MsG0280009613.01.T01 MTR_2g029960 59.722 72 29 0 153 224 462 533 2.97e-23 98.6
MsG0280009613.01.T01 MTR_1g112250 32.836 201 90 7 3 159 7 206 3.80e-23 94.7
MsG0280009613.01.T01 MTR_2g029960 60.563 71 28 0 153 223 452 522 4.58e-23 98.6
MsG0280009613.01.T01 MTR_2g029960 60.563 71 28 0 153 223 286 356 4.70e-23 98.2
MsG0280009613.01.T01 MTR_2g029960 60.563 71 28 0 153 223 322 392 4.71e-23 98.2
MsG0280009613.01.T01 MTR_2g094550 88.060 67 8 0 157 223 468 534 1.85e-19 87.8
MsG0280009613.01.T01 MTR_2g094550 88.060 67 8 0 157 223 468 534 1.91e-19 87.8
MsG0280009613.01.T01 MTR_1g022410 31.122 196 92 6 3 158 5 197 1.98e-19 85.5
MsG0280009613.01.T01 MTR_8g464460 29.954 217 106 7 4 175 9 224 1.74e-18 82.4
MsG0280009613.01.T01 MTR_2g060830 29.767 215 103 7 4 172 9 221 4.06e-18 81.3
MsG0280009613.01.T01 MTR_0167s0060 28.977 176 87 4 2 140 4 178 2.16e-16 76.3
MsG0280009613.01.T01 MTR_6g024135 44.578 83 45 1 4 86 8 89 9.32e-15 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009613.01.T01 AT5G66140 64.467 197 30 6 1 158 1 196 4.09e-74 226
MsG0280009613.01.T01 AT3G51260 63.452 197 32 6 1 158 1 196 5.64e-73 223
MsG0280009613.01.T01 AT3G51260 63.452 197 32 6 1 158 1 196 6.12e-73 223
MsG0280009613.01.T01 AT4G15165 38.065 155 91 2 3 156 4 154 8.53e-32 116
MsG0280009613.01.T01 AT1G07990 71.831 71 17 1 153 223 463 530 1.46e-28 114
MsG0280009613.01.T01 AT1G07990 71.831 71 17 1 153 223 463 530 1.54e-28 114
MsG0280009613.01.T01 AT3G45190 70.769 65 19 0 153 217 425 489 4.47e-27 110
MsG0280009613.01.T01 AT3G45190 70.769 65 19 0 153 217 463 527 5.25e-27 110
MsG0280009613.01.T01 AT3G22110 30.052 193 96 3 3 156 4 196 1.53e-26 103
MsG0280009613.01.T01 AT2G28360 73.846 65 14 1 153 217 463 524 1.32e-25 105
MsG0280009613.01.T01 AT1G53850 33.333 195 88 5 3 156 7 200 3.14e-24 97.8
MsG0280009613.01.T01 AT1G53850 33.333 195 88 5 3 156 7 200 3.14e-24 97.8
MsG0280009613.01.T01 AT3G14290 32.821 195 89 5 3 156 7 200 6.87e-24 96.7
MsG0280009613.01.T01 AT1G47250 32.143 196 90 6 3 158 5 197 3.54e-22 93.2
MsG0280009613.01.T01 AT5G35590 31.963 219 103 7 2 175 7 224 2.91e-21 89.7
MsG0280009613.01.T01 AT5G42790 31.633 196 91 6 3 158 5 197 1.15e-20 89.0
MsG0280009613.01.T01 AT2G05840 30.093 216 105 7 2 172 7 221 1.40e-18 82.8
MsG0280009613.01.T01 AT2G05840 30.622 209 99 7 2 165 7 214 4.65e-18 80.9
MsG0280009613.01.T01 AT1G16470 29.213 178 88 4 2 142 4 180 1.40e-17 79.7
MsG0280009613.01.T01 AT1G16470 29.213 178 88 4 2 142 4 180 1.40e-17 79.7
MsG0280009613.01.T01 AT1G79210 29.213 178 88 4 2 142 4 180 2.79e-17 79.0
MsG0280009613.01.T01 AT1G79210 29.213 178 88 4 2 142 4 180 2.79e-17 79.0
MsG0280009613.01.T01 AT1G79210 29.213 178 88 4 2 142 4 180 2.79e-17 79.0
MsG0280009613.01.T01 AT2G05840 30.000 180 86 5 2 142 7 185 3.38e-16 75.5
MsG0280009613.01.T01 AT2G27020 40.000 95 56 1 4 98 8 101 1.61e-14 71.6
MsG0280009613.01.T01 AT2G27020 40.000 95 56 1 4 98 110 203 2.32e-14 72.4
MsG0280009613.01.T01 AT1G30470 63.889 72 26 0 153 224 458 529 1.37e-11 64.7
MsG0280009613.01.T01 AT1G30470 63.889 72 26 0 153 224 464 535 1.42e-11 64.7
MsG0280009613.01.T01 AT1G30470 62.162 74 28 0 151 224 419 492 1.50e-11 64.7

Find 46 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGCAATTTGATCAAATTAT+TGG 0.148890 2:+55335509 None:intergenic
CCGCTAAGCTTCAAGATTCT+AGG 0.214824 2:-55337665 MsG0280009613.01.T01:intron
TCAATGCGTGCCCGTTTATC+AGG 0.273810 2:+55336910 None:intergenic
TGTTGTGCAGGTCGGTTGTT+AGG 0.352625 2:-55336998 MsG0280009613.01.T01:intron
ATGGCAAGCTACCGTGCTTC+AGG 0.362493 2:-55336173 MsG0280009613.01.T01:CDS
CAACGGTGAGTCTGTGACTC+TGG 0.413137 2:+55336884 None:intergenic
AGTACGCTCTAGAGGCTGTT+AGG 0.421329 2:-55337755 MsG0280009613.01.T01:CDS
CTGTACCAGACAGATCCTTC+TGG 0.439894 2:-55336725 MsG0280009613.01.T01:intron
GATGCTCGAGTCCTGATAAA+CGG 0.444046 2:-55336921 MsG0280009613.01.T01:CDS
TAACATTCTCAACCACATTA+CGG 0.444643 2:+55336149 None:intergenic
TCCTATCTTGTAAAGTAGTC+GGG 0.455210 2:+55335589 None:intergenic
TGCACTGTGGAGTATATTAC+CGG 0.455222 2:-55336853 MsG0280009613.01.T01:intron
AATTACAAAGAAAATGTTTG+AGG 0.457163 2:-55335393 None:intergenic
CGCTCTAGAGGCTGTTAGGA+AGG 0.457326 2:-55337751 MsG0280009613.01.T01:CDS
ATCCTATCTTGTAAAGTAGT+CGG 0.478725 2:+55335588 None:intergenic
ATGCTCGAGTCCTGATAAAC+GGG 0.481030 2:-55336920 MsG0280009613.01.T01:CDS
GTTATCGATAACATCGTTCT+CGG 0.481914 2:-55337705 MsG0280009613.01.T01:CDS
CACATTGCTCTTGCATGTGC+GGG 0.485966 2:-55336954 MsG0280009613.01.T01:CDS
GCCCGACTACTTTACAAGAT+AGG 0.487381 2:-55335590 MsG0280009613.01.T01:CDS
TGGACAGGAAACTGTGAAGT+TGG 0.502301 2:-55336615 MsG0280009613.01.T01:CDS
GCGTGGAAGGCAAATGCCAC+TGG 0.502652 2:-55336692 MsG0280009613.01.T01:CDS
CCTAGAATCTTGAAGCTTAG+CGG 0.516530 2:+55337665 None:intergenic
ATCGATAACGCGAACACCGA+CGG 0.519568 2:+55337719 None:intergenic
AACTCACTTCAAGCAAAGCT+CGG 0.527308 2:+55336588 None:intergenic
CTACTTTACAAGATAGGATG+AGG 0.536068 2:-55335584 MsG0280009613.01.T01:CDS
AAAATAGTGAGTGGAGTGAA+TGG 0.536200 2:-55336192 MsG0280009613.01.T01:CDS
ATGTTAACCGTTGGGCGTGT+GGG 0.540885 2:-55336132 MsG0280009613.01.T01:intron
GGTTGAGAATGTTAACCGTT+GGG 0.559190 2:-55336140 MsG0280009613.01.T01:CDS
TCAAGTCGAGTACGCTCTAG+AGG 0.560150 2:-55337763 MsG0280009613.01.T01:CDS
AATGTTAACCGTTGGGCGTG+TGG 0.566434 2:-55336133 MsG0280009613.01.T01:CDS
TTGATTGTGTTCAATAGCAT+AGG 0.569998 2:+55335465 None:intergenic
GTAATATACTCCACAGTGCA+TGG 0.582124 2:+55336856 None:intergenic
CACATTACGGTCCTGAAGCA+CGG 0.590677 2:+55336162 None:intergenic
TCACATTGCTCTTGCATGTG+CGG 0.602392 2:-55336955 MsG0280009613.01.T01:CDS
GCTCTAGAGGCTGTTAGGAA+GGG 0.603610 2:-55337750 MsG0280009613.01.T01:CDS
GAGCTTACCCACACGCCCAA+CGG 0.603966 2:+55336125 None:intergenic
CACAGTGCATGGATCTTCAA+CGG 0.606227 2:+55336867 None:intergenic
CGTGCTTCAGGACCGTAATG+TGG 0.613132 2:-55336161 MsG0280009613.01.T01:CDS
CACGCTGAAAATGTACCAGA+AGG 0.622171 2:+55336710 None:intergenic
AGAATCTTGAAGCTTAGCGG+TGG 0.631552 2:+55337668 None:intergenic
ACATGCAGGAAAATAGTGAG+TGG 0.642610 2:-55336201 MsG0280009613.01.T01:intron
TGGTTGAGAATGTTAACCGT+TGG 0.646731 2:-55336141 MsG0280009613.01.T01:CDS
TACTTTACAAGATAGGATGA+GGG 0.647749 2:-55335583 MsG0280009613.01.T01:CDS
AGGAAGGGTAACGCCACCGT+CGG 0.660774 2:-55337735 MsG0280009613.01.T01:CDS
CGTTGAAGATCCATGCACTG+TGG 0.702839 2:-55336866 MsG0280009613.01.T01:CDS
GATAACGCGAACACCGACGG+TGG 0.711586 2:+55337722 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTATATTCTGTTATTTAT+AGG - Chr2:55335959-55335978 MsG0280009613.01.T01:intron 10.0%
!! ATTCTATCATTCATTTATAT+AGG - Chr2:55336683-55336702 MsG0280009613.01.T01:CDS 15.0%
!! AAAGCTTATAACAAAATCAT+AGG + Chr2:55335687-55335706 None:intergenic 20.0%
!! AAGATGTAATACGTATAATA+TGG - Chr2:55337456-55337475 MsG0280009613.01.T01:intron 20.0%
!! AAGCTTATAACAAAATCATA+GGG + Chr2:55335686-55335705 None:intergenic 20.0%
!! AGAAGCTATTTGTTTAAATA+CGG - Chr2:55336030-55336049 MsG0280009613.01.T01:intron 20.0%
!! TAGCAAAGTAATAGAAATAA+AGG - Chr2:55335803-55335822 MsG0280009613.01.T01:intron 20.0%
!!! AACTGGCTTATAAAATAATA+TGG - Chr2:55336162-55336181 MsG0280009613.01.T01:CDS 20.0%
!!! ATTATTTTATAAGCCAGTTT+GGG + Chr2:55336161-55336180 None:intergenic 20.0%
!!! TATTATTTTATAAGCCAGTT+TGG + Chr2:55336162-55336181 None:intergenic 20.0%
!!! TGATTTTCATTTCTCAAATT+CGG - Chr2:55335604-55335623 MsG0280009613.01.T01:intron 20.0%
!!! TTTAGAATTTAGATCTAGTT+TGG - Chr2:55335849-55335868 MsG0280009613.01.T01:intron 20.0%
!!! TTTTTCTTATATATTCAACG+TGG - Chr2:55336979-55336998 MsG0280009613.01.T01:CDS 20.0%
! AACACTTCATAAGATATGTT+AGG - Chr2:55335773-55335792 MsG0280009613.01.T01:intron 25.0%
! AATGATTCTGTTAACTGTTA+AGG + Chr2:55336868-55336887 None:intergenic 25.0%
! CACATCATTATCATAACTAA+TGG + Chr2:55337398-55337417 None:intergenic 25.0%
! CTTGAGTTACTAAAATGTAA+AGG + Chr2:55337490-55337509 None:intergenic 25.0%
! GAAGCTATTTGTTTAAATAC+GGG - Chr2:55336031-55336050 MsG0280009613.01.T01:intron 25.0%
! GAGCAATTTGATCAAATTAT+TGG + Chr2:55337705-55337724 None:intergenic 25.0%
! TAAAGAAACAACATAAAGCA+CGG + Chr2:55335663-55335682 None:intergenic 25.0%
! TAACATATCTTATGAAGTGT+TGG + Chr2:55335774-55335793 None:intergenic 25.0%
! TATGATGTCAGTAAATAACT+CGG - Chr2:55335743-55335762 MsG0280009613.01.T01:intron 25.0%
! TCACAAAACTAAAACGTAAA+AGG + Chr2:55337536-55337555 None:intergenic 25.0%
!! ATCATTAGCAACATTTTACA+AGG - Chr2:55337134-55337153 MsG0280009613.01.T01:intron 25.0%
!! TTTTTCTTGCTAGAAGATAT+AGG - Chr2:55336730-55336749 MsG0280009613.01.T01:intron 25.0%
!!! AAGAATTTTAAAGAGACATC+TGG - Chr2:55336576-55336595 MsG0280009613.01.T01:intron 25.0%
!!! AATTCTTTTCTAGAAACTCA+AGG + Chr2:55336563-55336582 None:intergenic 25.0%
ACTACAACAATTAACACATC+AGG + Chr2:55335714-55335733 None:intergenic 30.0%
ATCCTATCTTGTAAAGTAGT+CGG + Chr2:55337626-55337645 None:intergenic 30.0%
GAATCATTTGACTGCAAATT+AGG - Chr2:55336880-55336899 MsG0280009613.01.T01:CDS 30.0%
GAGTTACTAAAATGTAAAGG+TGG + Chr2:55337487-55337506 None:intergenic 30.0%
TAACATTCTCAACCACATTA+CGG + Chr2:55337065-55337084 None:intergenic 30.0%
TACTTTACAAGATAGGATGA+GGG - Chr2:55337628-55337647 MsG0280009613.01.T01:intron 30.0%
TTGATTGTGTTCAATAGCAT+AGG + Chr2:55337749-55337768 None:intergenic 30.0%
TTTAAGAGTTTATCCCAAAC+TGG - Chr2:55336145-55336164 MsG0280009613.01.T01:CDS 30.0%
TTTCTATTACTTTGCTAGAC+AGG + Chr2:55335800-55335819 None:intergenic 30.0%
! AAGCAATCTAGAATGGATTT+AGG + Chr2:55336936-55336955 None:intergenic 30.0%
! AAGGTTTTGCAATTTTTGTG+CGG - Chr2:55335822-55335841 MsG0280009613.01.T01:intron 30.0%
! ATAAATACCACAAGCTTGTT+CGG + Chr2:55335945-55335964 None:intergenic 30.0%
! ATTAGCAACATTTTACAAGG+AGG - Chr2:55337137-55337156 MsG0280009613.01.T01:intron 30.0%
AAAATAGTGAGTGGAGTGAA+TGG - Chr2:55337019-55337038 MsG0280009613.01.T01:intron 35.0%
AAACGTAAAAGGATCAACCA+AGG + Chr2:55337525-55337544 None:intergenic 35.0%
AAGCTTCAAGATTCTAGGTT+TGG - Chr2:55335551-55335570 MsG0280009613.01.T01:CDS 35.0%
ACTTGAAAAAGATGACCATC+GGG + Chr2:55335434-55335453 None:intergenic 35.0%
AGTACTATAATTGTCAGCGT+AGG - Chr2:55337220-55337239 MsG0280009613.01.T01:intron 35.0%
ATCTTGACCAATAAGGGTTT+GGG - Chr2:55337278-55337297 MsG0280009613.01.T01:intron 35.0%
CTACTTTACAAGATAGGATG+AGG - Chr2:55337627-55337646 MsG0280009613.01.T01:intron 35.0%
CTCATGCATAAAGTCTTTGT+TGG - Chr2:55337179-55337198 MsG0280009613.01.T01:intron 35.0%
GACTTGAAAAAGATGACCAT+CGG + Chr2:55335435-55335454 None:intergenic 35.0%
GTTATCGATAACATCGTTCT+CGG - Chr2:55335506-55335525 MsG0280009613.01.T01:CDS 35.0%
TATCTTGACCAATAAGGGTT+TGG - Chr2:55337277-55337296 MsG0280009613.01.T01:intron 35.0%
TCAAGTGAAATCACAATCCT+TGG - Chr2:55337505-55337524 MsG0280009613.01.T01:intron 35.0%
TCATTTGACTGCAAATTAGG+AGG - Chr2:55336883-55336902 MsG0280009613.01.T01:CDS 35.0%
TCCTATCTTGTAAAGTAGTC+GGG + Chr2:55337625-55337644 None:intergenic 35.0%
TGCAAATTAGGAGGAAGTTT+AGG - Chr2:55336892-55336911 MsG0280009613.01.T01:CDS 35.0%
TTGTGAGAAGCAATCTAGAA+TGG + Chr2:55336943-55336962 None:intergenic 35.0%
! GTGTTAGGTTTAGGATCTTA+GGG - Chr2:55335626-55335645 MsG0280009613.01.T01:intron 35.0%
!! CATTTCTCAAATTCGGTGTT+AGG - Chr2:55335611-55335630 MsG0280009613.01.T01:intron 35.0%
!! CTTTGTTGGAAGTGTTGAAT+TGG - Chr2:55337193-55337212 MsG0280009613.01.T01:intron 35.0%
!! TCAAATTCGGTGTTAGGTTT+AGG - Chr2:55335617-55335636 MsG0280009613.01.T01:intron 35.0%
!! TTTTAAAGAGACATCTGGAC+AGG - Chr2:55336581-55336600 MsG0280009613.01.T01:intron 35.0%
!! TTTTATGACGTTGACTACCT+AGG - Chr2:55336766-55336785 MsG0280009613.01.T01:intron 35.0%
AACTCACTTCAAGCAAAGCT+CGG + Chr2:55336626-55336645 None:intergenic 40.0%
ACATGCAGGAAAATAGTGAG+TGG - Chr2:55337010-55337029 MsG0280009613.01.T01:intron 40.0%
ATCAAGTCCCAAACCCTTAT+TGG + Chr2:55337288-55337307 None:intergenic 40.0%
CCCTTATTGGTCAAGATAAC+CGG + Chr2:55337275-55337294 None:intergenic 40.0%
CCGGTTATCTTGACCAATAA+GGG - Chr2:55337272-55337291 MsG0280009613.01.T01:intron 40.0%
CCTAGAATCTTGAAGCTTAG+CGG + Chr2:55335549-55335568 None:intergenic 40.0%
CCTTATTGGTCAAGATAACC+GGG + Chr2:55337274-55337293 None:intergenic 40.0%
CTTGAAAAAGATGACCATCG+GGG + Chr2:55335433-55335452 None:intergenic 40.0%
GGTTGAGAATGTTAACCGTT+GGG - Chr2:55337071-55337090 MsG0280009613.01.T01:intron 40.0%
GTAATATACTCCACAGTGCA+TGG + Chr2:55336358-55336377 None:intergenic 40.0%
GTATATTACCGGTACATAGC+TGG - Chr2:55336369-55336388 MsG0280009613.01.T01:intron 40.0%
TGCACTGTGGAGTATATTAC+CGG - Chr2:55336358-55336377 MsG0280009613.01.T01:intron 40.0%
TGGTTGAGAATGTTAACCGT+TGG - Chr2:55337070-55337089 MsG0280009613.01.T01:intron 40.0%
TTGAAAAAGATGACCATCGG+GGG + Chr2:55335432-55335451 None:intergenic 40.0%
! GCAACATTTTACAAGGAGGT+TGG - Chr2:55337141-55337160 MsG0280009613.01.T01:intron 40.0%
! GGTGTTAGGTTTAGGATCTT+AGG - Chr2:55335625-55335644 MsG0280009613.01.T01:intron 40.0%
!! AATTTGCTGCAACTTGTGCA+AGG - Chr2:55336706-55336725 MsG0280009613.01.T01:CDS 40.0%
!! TCGTTTTGCTTTTGTTGTGC+AGG - Chr2:55336201-55336220 MsG0280009613.01.T01:intron 40.0%
!! TGAATTCGAGTTTTTGCCAG+TGG + Chr2:55336538-55336557 None:intergenic 40.0%
!! TTGGCTTTGATCCTTACACT+GGG - Chr2:55336454-55336473 MsG0280009613.01.T01:intron 40.0%
!! TTTGCTTTTGTTGTGCAGGT+CGG - Chr2:55336205-55336224 MsG0280009613.01.T01:intron 40.0%
ACAAAGACCGAACAAGCTTG+TGG - Chr2:55335935-55335954 MsG0280009613.01.T01:intron 45.0%
ACACCAACCATGTAAGGATG+TGG + Chr2:55337246-55337265 None:intergenic 45.0%
ACGTGGTGTTCTTAACATGC+AGG - Chr2:55336996-55337015 MsG0280009613.01.T01:intron 45.0%
ACTACCTAGGAGTGGTACTT+TGG - Chr2:55336779-55336798 MsG0280009613.01.T01:intron 45.0%
AGAATCTTGAAGCTTAGCGG+TGG + Chr2:55335546-55335565 None:intergenic 45.0%
AGGATCAAAGCCAACGATCA+AGG + Chr2:55336448-55336467 None:intergenic 45.0%
ATCAAAGCCAACGATCAAGG+TGG + Chr2:55336445-55336464 None:intergenic 45.0%
ATCAAGGTGGAAAGCCCAAA+TGG + Chr2:55336432-55336451 None:intergenic 45.0%
ATGCTCGAGTCCTGATAAAC+GGG - Chr2:55336291-55336310 MsG0280009613.01.T01:intron 45.0%
ATGTACCAGAAGGATCTGTC+TGG + Chr2:55336494-55336513 None:intergenic 45.0%
CACAGTGCATGGATCTTCAA+CGG + Chr2:55336347-55336366 None:intergenic 45.0%
CACGCTGAAAATGTACCAGA+AGG + Chr2:55336504-55336523 None:intergenic 45.0%
CCCGGTTATCTTGACCAATA+AGG - Chr2:55337271-55337290 MsG0280009613.01.T01:intron 45.0%
CCGCTAAGCTTCAAGATTCT+AGG - Chr2:55335546-55335565 MsG0280009613.01.T01:CDS 45.0%
GATGCTCGAGTCCTGATAAA+CGG - Chr2:55336290-55336309 MsG0280009613.01.T01:intron 45.0%
GCCCGACTACTTTACAAGAT+AGG - Chr2:55337621-55337640 MsG0280009613.01.T01:intron 45.0%
GTCACAACACCAACCATGTA+AGG + Chr2:55337252-55337271 None:intergenic 45.0%
TCACATTGCTCTTGCATGTG+CGG - Chr2:55336256-55336275 MsG0280009613.01.T01:intron 45.0%
TGGACAGGAAACTGTGAAGT+TGG - Chr2:55336596-55336615 MsG0280009613.01.T01:CDS 45.0%
! CAGAAGTACACACAGAGTGT+TGG - Chr2:55336399-55336418 MsG0280009613.01.T01:intron 45.0%
! CTTCTGGTACATTTTCAGCG+TGG - Chr2:55336502-55336521 MsG0280009613.01.T01:intron 45.0%
! TCTTCCAAAGTACCACTCCT+AGG + Chr2:55336786-55336805 None:intergenic 45.0%
! TGGTACATTTTCAGCGTGGA+AGG - Chr2:55336506-55336525 MsG0280009613.01.T01:intron 45.0%
!! GTTGGCTTTGATCCTTACAC+TGG - Chr2:55336453-55336472 MsG0280009613.01.T01:intron 45.0%
AATGTTAACCGTTGGGCGTG+TGG - Chr2:55337078-55337097 MsG0280009613.01.T01:intron 50.0%
AGGATCTGTCTGGTACAGAG+AGG + Chr2:55336484-55336503 None:intergenic 50.0%
AGGCCACATCCTTACATGGT+TGG - Chr2:55337240-55337259 MsG0280009613.01.T01:intron 50.0%
ATCGATAACGCGAACACCGA+CGG + Chr2:55335495-55335514 None:intergenic 50.0%
ATGTTAACCGTTGGGCGTGT+GGG - Chr2:55337079-55337098 MsG0280009613.01.T01:intron 50.0%
CACATTACGGTCCTGAAGCA+CGG + Chr2:55337052-55337071 None:intergenic 50.0%
CACATTGCTCTTGCATGTGC+GGG - Chr2:55336257-55336276 MsG0280009613.01.T01:intron 50.0%
CACTCTGTGTGTACTTCTGC+TGG + Chr2:55336398-55336417 None:intergenic 50.0%
CGTTGAAGATCCATGCACTG+TGG - Chr2:55336345-55336364 MsG0280009613.01.T01:intron 50.0%
CTGTACCAGACAGATCCTTC+TGG - Chr2:55336486-55336505 MsG0280009613.01.T01:intron 50.0%
GGATCTGTCTGGTACAGAGA+GGG + Chr2:55336483-55336502 None:intergenic 50.0%
TCAAGTCGAGTACGCTCTAG+AGG - Chr2:55335448-55335467 MsG0280009613.01.T01:CDS 50.0%
TCAATGCGTGCCCGTTTATC+AGG + Chr2:55336304-55336323 None:intergenic 50.0%
TGTTGTGCAGGTCGGTTGTT+AGG - Chr2:55336213-55336232 MsG0280009613.01.T01:intron 50.0%
! TGACGTTGACTACCTAGGAG+TGG - Chr2:55336771-55336790 MsG0280009613.01.T01:intron 50.0%
!! ACACACAGAGTGTTGGTGTG+CGG - Chr2:55336406-55336425 MsG0280009613.01.T01:intron 50.0%
!! ACATGGTTGGTGTTGTGACC+CGG - Chr2:55337253-55337272 MsG0280009613.01.T01:intron 50.0%
!! AGTACGCTCTAGAGGCTGTT+AGG - Chr2:55335456-55335475 MsG0280009613.01.T01:CDS 50.0%
!! GCTCTAGAGGCTGTTAGGAA+GGG - Chr2:55335461-55335480 MsG0280009613.01.T01:CDS 50.0%
AGAGAGGGAGACCCAGTGTA+AGG + Chr2:55336468-55336487 None:intergenic 55.0%
ATCACAGTCTTCACCCCCGA+TGG - Chr2:55335416-55335435 MsG0280009613.01.T01:CDS 55.0%
ATGGCAAGCTACCGTGCTTC+AGG - Chr2:55337038-55337057 MsG0280009613.01.T01:intron 55.0%
CAACGGTGAGTCTGTGACTC+TGG + Chr2:55336330-55336349 None:intergenic 55.0%
CGTGCTTCAGGACCGTAATG+TGG - Chr2:55337050-55337069 MsG0280009613.01.T01:intron 55.0%
GCGTAGGCCACATCCTTACA+TGG - Chr2:55337236-55337255 MsG0280009613.01.T01:intron 55.0%
GCTGGAGACCAGCTATGTAC+CGG + Chr2:55336380-55336399 None:intergenic 55.0%
GGGCTTTCCACCTTGATCGT+TGG - Chr2:55336435-55336454 MsG0280009613.01.T01:intron 55.0%
! GTGTTGGTGTGCGGCCATTT+GGG - Chr2:55336415-55336434 MsG0280009613.01.T01:intron 55.0%
!! AGTGTTGGTGTGCGGCCATT+TGG - Chr2:55336414-55336433 MsG0280009613.01.T01:intron 55.0%
!! CGCTCTAGAGGCTGTTAGGA+AGG - Chr2:55335460-55335479 MsG0280009613.01.T01:CDS 55.0%
AAGTAGTCGGGCGTCTGCAC+AGG + Chr2:55337613-55337632 None:intergenic 60.0%
AGGAAGGGTAACGCCACCGT+CGG - Chr2:55335476-55335495 MsG0280009613.01.T01:CDS 60.0%
GAGCTTACCCACACGCCCAA+CGG + Chr2:55337089-55337108 None:intergenic 60.0%
GATAACGCGAACACCGACGG+TGG + Chr2:55335492-55335511 None:intergenic 60.0%
GCGTGGAAGGCAAATGCCAC+TGG - Chr2:55336519-55336538 MsG0280009613.01.T01:intron 60.0%
TCGGGCGTCTGCACAGGAAA+AGG + Chr2:55337607-55337626 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 55335395 55337838 55335395 ID=MsG0280009613.01;Name=MsG0280009613.01
Chr2 mRNA 55335395 55337838 55335395 ID=MsG0280009613.01.T01;Parent=MsG0280009613.01;Name=MsG0280009613.01.T01;_AED=0.64;_eAED=0.64;_QI=0|0|0|0.6|1|1|5|0|258
Chr2 exon 55337666 55337838 55337666 ID=MsG0280009613.01.T01:exon:5959;Parent=MsG0280009613.01.T01
Chr2 exon 55336854 55337010 55336854 ID=MsG0280009613.01.T01:exon:5960;Parent=MsG0280009613.01.T01
Chr2 exon 55336595 55336738 55336595 ID=MsG0280009613.01.T01:exon:5961;Parent=MsG0280009613.01.T01
Chr2 exon 55336133 55336215 55336133 ID=MsG0280009613.01.T01:exon:5962;Parent=MsG0280009613.01.T01
Chr2 exon 55335395 55335614 55335395 ID=MsG0280009613.01.T01:exon:5963;Parent=MsG0280009613.01.T01
Chr2 CDS 55337666 55337838 55337666 ID=MsG0280009613.01.T01:cds;Parent=MsG0280009613.01.T01
Chr2 CDS 55336854 55337010 55336854 ID=MsG0280009613.01.T01:cds;Parent=MsG0280009613.01.T01
Chr2 CDS 55336595 55336738 55336595 ID=MsG0280009613.01.T01:cds;Parent=MsG0280009613.01.T01
Chr2 CDS 55336133 55336215 55336133 ID=MsG0280009613.01.T01:cds;Parent=MsG0280009613.01.T01
Chr2 CDS 55335395 55335614 55335395 ID=MsG0280009613.01.T01:cds;Parent=MsG0280009613.01.T01
Gene Sequence

>MsG0280009613.01.T01

ATGGCAAGATACGACAGAGCGATCACAGTCTTCACCCCCGATGGTCATCTTTTTCAAGTCGAGTACGCTCTAGAGGCTGTTAGGAAGGGTAACGCCACCGTCGGTGTTCGCGTTATCGATAACATCGTTCTCGGTGTTGAGAAGAAATCCACCGCTAAGCTTCAAGATTCTAGGTCGGTTGTTAGGAAGATTGTTAATCTAGATGATCACATTGCTCTTGCATGTGCGGGATTAAAAGCTGATGCTCGAGTCCTGATAAACGGGCACGCATTGAATGCCAGAGTCACAGACTCACCGTTGAAGATCCATGCACTGTGGAGTATATTACCGACAGATCCTTCTGGTACATTTTCAGCGTGGAAGGCAAATGCCACTGGCAAAAACTCGAATTCAATCCTTGAGTTTCTAGAAAAGAATTTTAAAGAGACATCTGGACAGGAAACTGTGAAGTTGGCTATCCGAGCTTTGCTTGAAGAAAATAGTGAGTGGAGTGAATGGCAAGCTACCGTGCTTCAGGACCGTAATGTGGTTGAGAATGTTAACCGTTGGGCGTGTGGACGCCCGACTACTTTACAAGATAGGATGAGGGATAGTGATGATGACGACATTCATGACAGAGACTATGATGTTGCAGCTTTAGCCAATAATTTGATCAAATTGCTCAAGCACAACTGCAAGAGACTTCCTATGCTATTGAACACAATCAAGAGACTTCTATTGCTCAAGCACAACTGCAAGAGACTTCTAATCAAACAAAATTACAAAGAAAATGTTTGA
Protein sequence

>MsG0280009613.01.T01

MARYDRAITVFTPDGHLFQVEYALEAVRKGNATVGVRVIDNIVLGVEKKSTAKLQDSRSVVRKIVNLDDHIALACAGLKADARVLINGHALNARVTDSPLKIHALWSILPTDPSGTFSAWKANATGKNSNSILEFLEKNFKETSGQETVKLAIRALLEENSEWSEWQATVLQDRNVVENVNRWACGRPTTLQDRMRDSDDDDIHDRDYDVAALANNLIKLLKHNCKRLPMLLNTIKRLLLLKHNCKRLLIKQNYKENV*